Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77972.t1 | XP_003590374.1 | 93.8 | 112 | 7 | 0 | 1 | 112 | 1 | 112 | 2.90E-53 | 217.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77972.t1 | G7I999 | 93.8 | 112 | 7 | 0 | 1 | 112 | 1 | 112 | 2.1e-53 | 217.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049230 | MS.gene77972 | -0.831314 | 1.79E-55 | -1.69E-46 |
| MS.gene049378 | MS.gene77972 | -0.875995 | 1.98E-68 | -1.69E-46 |
| MS.gene049669 | MS.gene77972 | -0.807565 | 4.75E-50 | -1.69E-46 |
| MS.gene04991 | MS.gene77972 | 0.868435 | 6.55E-66 | -1.69E-46 |
| MS.gene050105 | MS.gene77972 | -0.824729 | 6.89E-54 | -1.69E-46 |
| MS.gene050227 | MS.gene77972 | -0.814007 | 1.92E-51 | -1.69E-46 |
| MS.gene050228 | MS.gene77972 | -0.816479 | 5.41E-52 | -1.69E-46 |
| MS.gene050623 | MS.gene77972 | 0.84845 | 6.08E-60 | -1.69E-46 |
| MS.gene051064 | MS.gene77972 | -0.83074 | 2.48E-55 | -1.69E-46 |
| MS.gene051122 | MS.gene77972 | -0.843035 | 1.79E-58 | -1.69E-46 |
| MS.gene051368 | MS.gene77972 | -0.806192 | 9.27E-50 | -1.69E-46 |
| MS.gene051983 | MS.gene77972 | -0.820439 | 6.86E-53 | -1.69E-46 |
| MS.gene05216 | MS.gene77972 | -0.805182 | 1.51E-49 | -1.69E-46 |
| MS.gene052176 | MS.gene77972 | -0.80444 | 2.16E-49 | -1.69E-46 |
| MS.gene052333 | MS.gene77972 | -0.80436 | 2.24E-49 | -1.69E-46 |
| MS.gene052389 | MS.gene77972 | -0.842817 | 2.05E-58 | -1.69E-46 |
| MS.gene052420 | MS.gene77972 | -0.826283 | 2.95E-54 | -1.69E-46 |
| MS.gene052464 | MS.gene77972 | -0.807379 | 5.20E-50 | -1.69E-46 |
| MS.gene052586 | MS.gene77972 | -0.82619 | 3.11E-54 | -1.69E-46 |
| MS.gene052674 | MS.gene77972 | -0.800865 | 1.18E-48 | -1.69E-46 |
| MS.gene052677 | MS.gene77972 | -0.803632 | 3.18E-49 | -1.69E-46 |
| MS.gene054468 | MS.gene77972 | -0.802485 | 5.49E-49 | -1.69E-46 |
| MS.gene054479 | MS.gene77972 | -0.836423 | 9.44E-57 | -1.69E-46 |
| MS.gene054930 | MS.gene77972 | -0.805467 | 1.32E-49 | -1.69E-46 |
| MS.gene055225 | MS.gene77972 | -0.807509 | 4.88E-50 | -1.69E-46 |
| MS.gene055701 | MS.gene77972 | -0.865799 | 4.54E-65 | -1.69E-46 |
| MS.gene05595 | MS.gene77972 | -0.822298 | 2.55E-53 | -1.69E-46 |
| MS.gene056979 | MS.gene77972 | -0.812707 | 3.70E-51 | -1.69E-46 |
| MS.gene057156 | MS.gene77972 | 0.830345 | 3.09E-55 | -1.69E-46 |
| MS.gene05761 | MS.gene77972 | -0.83247 | 9.28E-56 | -1.69E-46 |
| MS.gene058223 | MS.gene77972 | 0.856089 | 4.07E-62 | -1.69E-46 |
| MS.gene05843 | MS.gene77972 | 0.815927 | 7.19E-52 | -1.69E-46 |
| MS.gene060850 | MS.gene77972 | -0.815566 | 8.66E-52 | -1.69E-46 |
| MS.gene060851 | MS.gene77972 | -0.846294 | 2.38E-59 | -1.69E-46 |
| MS.gene060852 | MS.gene77972 | -0.845501 | 3.90E-59 | -1.69E-46 |
| MS.gene060879 | MS.gene77972 | -0.828703 | 7.76E-55 | -1.69E-46 |
| MS.gene060940 | MS.gene77972 | -0.81184 | 5.72E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77972.t1 | MTR_1g059290 | 93.750 | 112 | 7 | 0 | 1 | 112 | 1 | 112 | 4.90e-66 | 195 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77972.t1 | AT2G42310 | 68.932 | 103 | 29 | 1 | 2 | 104 | 14 | 113 | 7.69e-50 | 154 |
| MS.gene77972.t1 | AT3G57785 | 71.000 | 100 | 28 | 1 | 6 | 104 | 14 | 113 | 2.51e-49 | 152 |
Find 44 sgRNAs with CRISPR-Local
Find 42 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCCGTCTCCGCGGCGGTTC+AGG | 0.224776 | 1.4:-41824701 | MS.gene77972:CDS |
| TCAAATCAGGCTTAGCATTA+AGG | 0.309891 | 1.4:+41824516 | None:intergenic |
| AGCCCTTGAACGTCTCAAAT+TGG | 0.351342 | 1.4:-41824468 | MS.gene77972:CDS |
| CTGATTTGACTATTGAAACT+TGG | 0.354374 | 1.4:-41824502 | MS.gene77972:CDS |
| TAGGTCTTTCTTCGTGGCCT+GGG | 0.366840 | 1.4:+41824654 | None:intergenic |
| TTAGGTCTTTCTTCGTGGCC+TGG | 0.383907 | 1.4:+41824653 | None:intergenic |
| TGGTTGAAGAGGTAGCCTTT+AGG | 0.396876 | 1.4:+41824635 | None:intergenic |
| CAAGTTTCAATAGTCAAATC+AGG | 0.400055 | 1.4:+41824503 | None:intergenic |
| CTCCGCGGCGGTTCAGGAAC+CGG | 0.401455 | 1.4:-41824695 | MS.gene77972:CDS |
| CACCTGGAGCGAGTGGTGTT+TGG | 0.403944 | 1.4:+41824615 | None:intergenic |
| AATCACGCAAATGGGAAGAT+TGG | 0.415297 | 1.4:-41824592 | MS.gene77972:CDS |
| CTCCAGGTGAATCACGCAAA+TGG | 0.420212 | 1.4:-41824601 | MS.gene77972:CDS |
| CTTCCAATTTGAGACGTTCA+AGG | 0.438923 | 1.4:+41824465 | None:intergenic |
| TCCCATTTGCGTGATTCACC+TGG | 0.448242 | 1.4:+41824599 | None:intergenic |
| ATCACGCAAATGGGAAGATT+GGG | 0.466913 | 1.4:-41824591 | MS.gene77972:CDS |
| CGAGTGGTGTTTGGTTGAAG+AGG | 0.470232 | 1.4:+41824624 | None:intergenic |
| CGTGGCCTGGGCTTGTCCAT+CGG | 0.482648 | 1.4:+41824666 | None:intergenic |
| GCTTGTCCATCGGCTTGCAC+CGG | 0.486213 | 1.4:+41824676 | None:intergenic |
| TCCAGGTGAATCACGCAAAT+GGG | 0.489078 | 1.4:-41824600 | MS.gene77972:CDS |
| TGATTTGACTATTGAAACTT+GGG | 0.489933 | 1.4:-41824501 | MS.gene77972:CDS |
| AACCAAACACCACTCGCTCC+AGG | 0.505049 | 1.4:-41824617 | MS.gene77972:CDS |
| AGCCTTTAGGTCTTTCTTCG+TGG | 0.508189 | 1.4:+41824648 | None:intergenic |
| GCAAGCCGATGGACAAGCCC+AGG | 0.525769 | 1.4:-41824671 | MS.gene77972:CDS |
| GAGGAGACGTTGGCGGAGCA+TGG | 0.529777 | 1.4:+41824730 | None:intergenic |
| TTCCTGAACCGCCGCGGAGA+CGG | 0.531358 | 1.4:+41824699 | None:intergenic |
| TTCCTCACCATCGTTATCCT+CGG | 0.531600 | 1.4:-41824545 | MS.gene77972:CDS |
| TCAAAATCATCTCTATTCAT+CGG | 0.537178 | 1.4:+41824400 | None:intergenic |
| ACCATCGTTATCCTCGGTGT+TGG | 0.550397 | 1.4:-41824539 | MS.gene77972:CDS |
| CAGGAACCGGTGCAAGCCGA+TGG | 0.563436 | 1.4:-41824682 | MS.gene77972:CDS |
| TTCCAATTTGAGACGTTCAA+GGG | 0.567951 | 1.4:+41824466 | None:intergenic |
| GGAGAGAGGTGAGGAGACGT+TGG | 0.578698 | 1.4:+41824720 | None:intergenic |
| GAGAGGTGAGGAGACGTTGG+CGG | 0.579804 | 1.4:+41824723 | None:intergenic |
| GGCCACGAAGAAAGACCTAA+AGG | 0.583150 | 1.4:-41824650 | MS.gene77972:CDS |
| CGCGGAGACGGAGAGAGGTG+AGG | 0.590890 | 1.4:+41824711 | None:intergenic |
| ACCTCTCTCCGTCTCCGCGG+CGG | 0.593678 | 1.4:-41824707 | MS.gene77972:CDS |
| ACCGCCGCGGAGACGGAGAG+AGG | 0.601010 | 1.4:+41824706 | None:intergenic |
| CGTGATTCACCTGGAGCGAG+TGG | 0.616656 | 1.4:+41824608 | None:intergenic |
| AGGAAGCTAGTGATGTAGCA+AGG | 0.627360 | 1.4:+41824563 | None:intergenic |
| CACCGAGGATAACGATGGTG+AGG | 0.629292 | 1.4:+41824543 | None:intergenic |
| CACCGGTTCCTGAACCGCCG+CGG | 0.646325 | 1.4:+41824693 | None:intergenic |
| GGAATCATCAACGACGACAG+CGG | 0.672278 | 1.4:+41824421 | None:intergenic |
| GCCAACACCGAGGATAACGA+TGG | 0.718051 | 1.4:+41824538 | None:intergenic |
| TAGCATTAAGGCCAACACCG+AGG | 0.728768 | 1.4:+41824528 | None:intergenic |
| CTCACCTCTCTCCGTCTCCG+CGG | 0.734494 | 1.4:-41824710 | MS.gene77972:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TGATTTGACTATTGAAACTT+GGG | - | chr1.4:41824639-41824658 | MS.gene77972:CDS | 25.0% |
| CAAGTTTCAATAGTCAAATC+AGG | + | chr1.4:41824640-41824659 | None:intergenic | 30.0% | |
| ! | CTGATTTGACTATTGAAACT+TGG | - | chr1.4:41824638-41824657 | MS.gene77972:CDS | 30.0% |
| TCAAATCAGGCTTAGCATTA+AGG | + | chr1.4:41824627-41824646 | None:intergenic | 35.0% | |
| ! | TTCCAATTTGAGACGTTCAA+GGG | + | chr1.4:41824677-41824696 | None:intergenic | 35.0% |
| AATCACGCAAATGGGAAGAT+TGG | - | chr1.4:41824548-41824567 | MS.gene77972:CDS | 40.0% | |
| ATCACGCAAATGGGAAGATT+GGG | - | chr1.4:41824549-41824568 | MS.gene77972:CDS | 40.0% | |
| ! | CTTCCAATTTGAGACGTTCA+AGG | + | chr1.4:41824678-41824697 | None:intergenic | 40.0% |
| AGCCCTTGAACGTCTCAAAT+TGG | - | chr1.4:41824672-41824691 | MS.gene77972:CDS | 45.0% | |
| AGCCTTTAGGTCTTTCTTCG+TGG | + | chr1.4:41824495-41824514 | None:intergenic | 45.0% | |
| AGGAAGCTAGTGATGTAGCA+AGG | + | chr1.4:41824580-41824599 | None:intergenic | 45.0% | |
| TCCAGGTGAATCACGCAAAT+GGG | - | chr1.4:41824540-41824559 | MS.gene77972:CDS | 45.0% | |
| TGGTTGAAGAGGTAGCCTTT+AGG | + | chr1.4:41824508-41824527 | None:intergenic | 45.0% | |
| TTCCTCACCATCGTTATCCT+CGG | - | chr1.4:41824595-41824614 | MS.gene77972:CDS | 45.0% | |
| CGAGTGGTGTTTGGTTGAAG+AGG | + | chr1.4:41824519-41824538 | None:intergenic | 50.0% | |
| CTCCAGGTGAATCACGCAAA+TGG | - | chr1.4:41824539-41824558 | MS.gene77972:CDS | 50.0% | |
| GGAATCATCAACGACGACAG+CGG | + | chr1.4:41824722-41824741 | None:intergenic | 50.0% | |
| GGCCACGAAGAAAGACCTAA+AGG | - | chr1.4:41824490-41824509 | MS.gene77972:CDS | 50.0% | |
| TAGCATTAAGGCCAACACCG+AGG | + | chr1.4:41824615-41824634 | None:intergenic | 50.0% | |
| TAGGTCTTTCTTCGTGGCCT+GGG | + | chr1.4:41824489-41824508 | None:intergenic | 50.0% | |
| TCCCATTTGCGTGATTCACC+TGG | + | chr1.4:41824544-41824563 | None:intergenic | 50.0% | |
| TTAGGTCTTTCTTCGTGGCC+TGG | + | chr1.4:41824490-41824509 | None:intergenic | 50.0% | |
| ! | ACCATCGTTATCCTCGGTGT+TGG | - | chr1.4:41824601-41824620 | MS.gene77972:CDS | 50.0% |
| AACCAAACACCACTCGCTCC+AGG | - | chr1.4:41824523-41824542 | MS.gene77972:CDS | 55.0% | |
| CACCGAGGATAACGATGGTG+AGG | + | chr1.4:41824600-41824619 | None:intergenic | 55.0% | |
| GCCAACACCGAGGATAACGA+TGG | + | chr1.4:41824605-41824624 | None:intergenic | 55.0% | |
| CACCTGGAGCGAGTGGTGTT+TGG | + | chr1.4:41824528-41824547 | None:intergenic | 60.0% | |
| CGTGATTCACCTGGAGCGAG+TGG | + | chr1.4:41824535-41824554 | None:intergenic | 60.0% | |
| GAGAGGTGAGGAGACGTTGG+CGG | + | chr1.4:41824420-41824439 | None:intergenic | 60.0% | |
| GGAGAGAGGTGAGGAGACGT+TGG | + | chr1.4:41824423-41824442 | None:intergenic | 60.0% | |
| !! | GCTTGTCCATCGGCTTGCAC+CGG | + | chr1.4:41824467-41824486 | None:intergenic | 60.0% |
| CAGGAACCGGTGCAAGCCGA+TGG | - | chr1.4:41824458-41824477 | MS.gene77972:CDS | 65.0% | |
| CGTGGCCTGGGCTTGTCCAT+CGG | + | chr1.4:41824477-41824496 | None:intergenic | 65.0% | |
| CTCACCTCTCTCCGTCTCCG+CGG | - | chr1.4:41824430-41824449 | MS.gene77972:CDS | 65.0% | |
| GCAAGCCGATGGACAAGCCC+AGG | - | chr1.4:41824469-41824488 | MS.gene77972:CDS | 65.0% | |
| TTCCTGAACCGCCGCGGAGA+CGG | + | chr1.4:41824444-41824463 | None:intergenic | 65.0% | |
| ACCTCTCTCCGTCTCCGCGG+CGG | - | chr1.4:41824433-41824452 | MS.gene77972:CDS | 70.0% | |
| CGCGGAGACGGAGAGAGGTG+AGG | + | chr1.4:41824432-41824451 | None:intergenic | 70.0% | |
| CTCCGCGGCGGTTCAGGAAC+CGG | - | chr1.4:41824445-41824464 | MS.gene77972:CDS | 70.0% | |
| !! | CACCGGTTCCTGAACCGCCG+CGG | + | chr1.4:41824450-41824469 | None:intergenic | 70.0% |
| ACCGCCGCGGAGACGGAGAG+AGG | + | chr1.4:41824437-41824456 | None:intergenic | 75.0% | |
| CTCCGTCTCCGCGGCGGTTC+AGG | - | chr1.4:41824439-41824458 | MS.gene77972:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 41824412 | 41824750 | 41824412 | ID=MS.gene77972 |
| chr1.4 | mRNA | 41824412 | 41824750 | 41824412 | ID=MS.gene77972.t1;Parent=MS.gene77972 |
| chr1.4 | exon | 41824412 | 41824750 | 41824412 | ID=MS.gene77972.t1.exon1;Parent=MS.gene77972.t1 |
| chr1.4 | CDS | 41824412 | 41824750 | 41824412 | ID=cds.MS.gene77972.t1;Parent=MS.gene77972.t1 |
| Gene Sequence |
| Protein sequence |
