Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79178.t1 | XP_013448012.1 | 98.1 | 378 | 7 | 0 | 1 | 378 | 28 | 405 | 4.60E-220 | 773.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79178.t1 | Q9LI89 | 48.7 | 388 | 183 | 5 | 4 | 375 | 34 | 421 | 1.4e-95 | 351.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79178.t1 | A0A072TX07 | 98.1 | 378 | 7 | 0 | 1 | 378 | 28 | 405 | 3.3e-220 | 773.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049171 | MS.gene79178 | -0.808098 | 3.66E-50 | -1.69E-46 |
| MS.gene049281 | MS.gene79178 | 0.867607 | 1.21E-65 | -1.69E-46 |
| MS.gene050170 | MS.gene79178 | -0.807829 | 4.18E-50 | -1.69E-46 |
| MS.gene050496 | MS.gene79178 | -0.832262 | 1.05E-55 | -1.69E-46 |
| MS.gene050504 | MS.gene79178 | -0.820664 | 6.09E-53 | -1.69E-46 |
| MS.gene050612 | MS.gene79178 | -0.807168 | 5.77E-50 | -1.69E-46 |
| MS.gene050699 | MS.gene79178 | -0.800707 | 1.27E-48 | -1.69E-46 |
| MS.gene050883 | MS.gene79178 | 0.834326 | 3.20E-56 | -1.69E-46 |
| MS.gene051044 | MS.gene79178 | 0.825147 | 5.49E-54 | -1.69E-46 |
| MS.gene051127 | MS.gene79178 | 0.834325 | 3.20E-56 | -1.69E-46 |
| MS.gene051364 | MS.gene79178 | 0.842482 | 2.52E-58 | -1.69E-46 |
| MS.gene051366 | MS.gene79178 | 0.838892 | 2.19E-57 | -1.69E-46 |
| MS.gene051367 | MS.gene79178 | 0.809166 | 2.16E-50 | -1.69E-46 |
| MS.gene051422 | MS.gene79178 | 0.817736 | 2.83E-52 | -1.69E-46 |
| MS.gene052482 | MS.gene79178 | -0.800073 | 1.71E-48 | -1.69E-46 |
| MS.gene053420 | MS.gene79178 | 0.844222 | 8.64E-59 | -1.69E-46 |
| MS.gene053826 | MS.gene79178 | 0.807305 | 5.39E-50 | -1.69E-46 |
| MS.gene054708 | MS.gene79178 | 0.804264 | 2.35E-49 | -1.69E-46 |
| MS.gene05470 | MS.gene79178 | -0.817098 | 3.93E-52 | -1.69E-46 |
| MS.gene054954 | MS.gene79178 | -0.814952 | 1.19E-51 | -1.69E-46 |
| MS.gene054959 | MS.gene79178 | 0.842798 | 2.07E-58 | -1.69E-46 |
| MS.gene055154 | MS.gene79178 | 0.822839 | 1.91E-53 | -1.69E-46 |
| MS.gene055362 | MS.gene79178 | -0.802937 | 4.43E-49 | -1.69E-46 |
| MS.gene055502 | MS.gene79178 | -0.813139 | 2.98E-51 | -1.69E-46 |
| MS.gene05589 | MS.gene79178 | -0.802686 | 4.99E-49 | -1.69E-46 |
| MS.gene055942 | MS.gene79178 | 0.836822 | 7.47E-57 | -1.69E-46 |
| MS.gene056198 | MS.gene79178 | 0.813068 | 3.09E-51 | -1.69E-46 |
| MS.gene056200 | MS.gene79178 | 0.810985 | 8.78E-51 | -1.69E-46 |
| MS.gene056504 | MS.gene79178 | -0.825417 | 4.74E-54 | -1.69E-46 |
| MS.gene056748 | MS.gene79178 | 0.810431 | 1.16E-50 | -1.69E-46 |
| MS.gene056761 | MS.gene79178 | 0.808389 | 3.17E-50 | -1.69E-46 |
| MS.gene056856 | MS.gene79178 | 0.805861 | 1.09E-49 | -1.69E-46 |
| MS.gene057499 | MS.gene79178 | 0.82826 | 9.93E-55 | -1.69E-46 |
| MS.gene057548 | MS.gene79178 | 0.818689 | 1.72E-52 | -1.69E-46 |
| MS.gene057768 | MS.gene79178 | 0.80618 | 9.32E-50 | -1.69E-46 |
| MS.gene058099 | MS.gene79178 | 0.813397 | 2.61E-51 | -1.69E-46 |
| MS.gene058111 | MS.gene79178 | 0.823489 | 1.35E-53 | -1.69E-46 |
| MS.gene058268 | MS.gene79178 | 0.828032 | 1.13E-54 | -1.69E-46 |
| MS.gene058269 | MS.gene79178 | 0.802595 | 5.21E-49 | -1.69E-46 |
| MS.gene058419 | MS.gene79178 | -0.804069 | 2.58E-49 | -1.69E-46 |
| MS.gene058493 | MS.gene79178 | 0.809231 | 2.10E-50 | -1.69E-46 |
| MS.gene058494 | MS.gene79178 | 0.800846 | 1.19E-48 | -1.69E-46 |
| MS.gene058603 | MS.gene79178 | 0.800913 | 1.15E-48 | -1.69E-46 |
| MS.gene058630 | MS.gene79178 | 0.801414 | 9.11E-49 | -1.69E-46 |
| MS.gene059022 | MS.gene79178 | -0.825926 | 3.59E-54 | -1.69E-46 |
| MS.gene059677 | MS.gene79178 | -0.809851 | 1.54E-50 | -1.69E-46 |
| MS.gene059770 | MS.gene79178 | 0.806473 | 8.09E-50 | -1.69E-46 |
| MS.gene059865 | MS.gene79178 | -0.805393 | 1.36E-49 | -1.69E-46 |
| MS.gene060595 | MS.gene79178 | 0.832968 | 6.98E-56 | -1.69E-46 |
| MS.gene06077 | MS.gene79178 | -0.832811 | 7.64E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79178.t1 | MTR_7g029290 | 98.148 | 378 | 7 | 0 | 1 | 378 | 28 | 405 | 0.0 | 765 |
| MS.gene79178.t1 | MTR_6g023805 | 63.918 | 388 | 118 | 6 | 1 | 376 | 28 | 405 | 6.22e-167 | 473 |
| MS.gene79178.t1 | MTR_2g094093 | 38.841 | 345 | 203 | 6 | 32 | 374 | 24 | 362 | 4.13e-76 | 239 |
| MS.gene79178.t1 | MTR_4g108280 | 36.119 | 371 | 214 | 10 | 18 | 374 | 17 | 378 | 1.35e-70 | 226 |
| MS.gene79178.t1 | MTR_0345s0010 | 37.466 | 371 | 213 | 11 | 13 | 374 | 73 | 433 | 1.38e-69 | 225 |
| MS.gene79178.t1 | MTR_0105s0020 | 37.466 | 371 | 213 | 11 | 13 | 374 | 73 | 433 | 2.79e-69 | 224 |
| MS.gene79178.t1 | MTR_0105s0130 | 37.466 | 371 | 213 | 11 | 13 | 374 | 73 | 433 | 6.03e-69 | 223 |
| MS.gene79178.t1 | MTR_0105s0170 | 37.197 | 371 | 214 | 11 | 13 | 374 | 86 | 446 | 3.66e-68 | 222 |
| MS.gene79178.t1 | MTR_0496s0010 | 37.566 | 378 | 197 | 13 | 13 | 374 | 55 | 409 | 4.23e-68 | 220 |
| MS.gene79178.t1 | MTR_0105s0040 | 37.197 | 371 | 214 | 11 | 13 | 374 | 171 | 531 | 7.74e-68 | 223 |
| MS.gene79178.t1 | MTR_4g022260 | 36.508 | 378 | 207 | 13 | 13 | 374 | 55 | 415 | 3.77e-67 | 218 |
| MS.gene79178.t1 | MTR_5g039390 | 37.173 | 382 | 219 | 11 | 5 | 374 | 102 | 474 | 4.30e-67 | 219 |
| MS.gene79178.t1 | MTR_8g468030 | 38.663 | 344 | 194 | 9 | 39 | 374 | 161 | 495 | 5.72e-67 | 220 |
| MS.gene79178.t1 | MTR_1330s0010 | 37.571 | 354 | 204 | 10 | 13 | 357 | 86 | 431 | 1.67e-66 | 217 |
| MS.gene79178.t1 | MTR_4g022400 | 35.342 | 365 | 219 | 9 | 18 | 374 | 57 | 412 | 2.19e-66 | 216 |
| MS.gene79178.t1 | MTR_8g085600 | 27.303 | 304 | 200 | 10 | 5 | 299 | 55 | 346 | 4.32e-24 | 103 |
| MS.gene79178.t1 | MTR_4g114110 | 25.168 | 298 | 198 | 10 | 9 | 298 | 69 | 349 | 1.35e-18 | 87.4 |
| MS.gene79178.t1 | MTR_3g073640 | 25.652 | 230 | 156 | 6 | 31 | 254 | 46 | 266 | 3.48e-16 | 80.1 |
| MS.gene79178.t1 | MTR_7g095220 | 25.957 | 235 | 145 | 8 | 32 | 248 | 8 | 231 | 8.08e-15 | 75.1 |
| MS.gene79178.t1 | MTR_3g085570 | 24.272 | 309 | 201 | 11 | 32 | 325 | 39 | 329 | 3.18e-13 | 70.9 |
| MS.gene79178.t1 | MTR_2g019230 | 23.427 | 286 | 198 | 9 | 32 | 304 | 56 | 333 | 1.62e-11 | 65.9 |
| MS.gene79178.t1 | MTR_2g008440 | 25.272 | 368 | 200 | 14 | 31 | 326 | 46 | 410 | 1.91e-11 | 65.9 |
| MS.gene79178.t1 | MTR_2g012790 | 28.485 | 165 | 102 | 3 | 138 | 292 | 109 | 267 | 2.51e-11 | 65.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79178.t1 | AT3G27150 | 51.928 | 389 | 171 | 5 | 4 | 376 | 34 | 422 | 4.43e-133 | 387 |
| MS.gene79178.t1 | AT3G27150 | 51.928 | 389 | 171 | 5 | 4 | 376 | 34 | 422 | 4.43e-133 | 387 |
| MS.gene79178.t1 | AT5G40680 | 47.757 | 379 | 192 | 4 | 4 | 376 | 37 | 415 | 1.83e-119 | 352 |
| MS.gene79178.t1 | AT5G60570 | 39.884 | 346 | 198 | 7 | 32 | 374 | 53 | 391 | 3.48e-72 | 231 |
| MS.gene79178.t1 | AT5G60570 | 39.884 | 346 | 198 | 7 | 32 | 374 | 53 | 391 | 3.48e-72 | 231 |
| MS.gene79178.t1 | AT5G60570 | 39.884 | 346 | 198 | 7 | 32 | 374 | 53 | 391 | 3.48e-72 | 231 |
| MS.gene79178.t1 | AT1G14330 | 39.650 | 343 | 192 | 9 | 39 | 374 | 105 | 439 | 7.83e-70 | 226 |
| MS.gene79178.t1 | AT2G02870 | 37.662 | 385 | 210 | 12 | 12 | 374 | 89 | 465 | 3.55e-67 | 219 |
| MS.gene79178.t1 | AT2G02870 | 37.662 | 385 | 210 | 12 | 12 | 374 | 89 | 465 | 3.55e-67 | 219 |
| MS.gene79178.t1 | AT2G02870 | 37.662 | 385 | 210 | 12 | 12 | 374 | 89 | 465 | 3.55e-67 | 219 |
| MS.gene79178.t1 | AT1G74510 | 37.288 | 354 | 190 | 9 | 41 | 374 | 108 | 449 | 4.76e-64 | 211 |
| MS.gene79178.t1 | AT1G74510 | 37.288 | 354 | 190 | 9 | 41 | 374 | 108 | 449 | 4.76e-64 | 211 |
| MS.gene79178.t1 | AT1G74510 | 37.288 | 354 | 190 | 9 | 41 | 374 | 108 | 449 | 4.76e-64 | 211 |
| MS.gene79178.t1 | AT1G26930 | 36.074 | 377 | 207 | 10 | 13 | 374 | 62 | 419 | 2.19e-63 | 208 |
| MS.gene79178.t1 | AT1G26930 | 30.769 | 377 | 175 | 12 | 13 | 374 | 62 | 367 | 2.13e-41 | 149 |
| MS.gene79178.t1 | AT1G55270 | 26.812 | 276 | 184 | 8 | 32 | 299 | 82 | 347 | 3.82e-20 | 92.0 |
| MS.gene79178.t1 | AT4G35120 | 28.241 | 216 | 139 | 6 | 31 | 234 | 29 | 240 | 7.22e-13 | 69.7 |
| MS.gene79178.t1 | AT3G63220 | 24.756 | 307 | 184 | 14 | 41 | 328 | 17 | 295 | 1.04e-12 | 68.9 |
| MS.gene79178.t1 | AT3G63220 | 23.810 | 315 | 195 | 13 | 32 | 328 | 15 | 302 | 1.09e-12 | 68.9 |
| MS.gene79178.t1 | AT3G63220 | 23.810 | 315 | 195 | 13 | 32 | 328 | 15 | 302 | 1.09e-12 | 68.9 |
| MS.gene79178.t1 | AT1G60570 | 25.574 | 305 | 197 | 10 | 10 | 299 | 2 | 291 | 4.97e-12 | 67.0 |
| MS.gene79178.t1 | AT1G22040 | 26.180 | 233 | 150 | 7 | 31 | 254 | 46 | 265 | 2.30e-11 | 65.5 |
| MS.gene79178.t1 | AT4G29370 | 29.000 | 200 | 119 | 8 | 17 | 204 | 14 | 202 | 5.00e-11 | 63.9 |
| MS.gene79178.t1 | AT1G55270 | 25.500 | 200 | 134 | 7 | 105 | 299 | 32 | 221 | 5.31e-11 | 63.5 |
| MS.gene79178.t1 | AT1G16250 | 22.074 | 376 | 258 | 10 | 32 | 378 | 12 | 381 | 5.78e-11 | 63.9 |
| MS.gene79178.t1 | AT1G16250 | 22.074 | 376 | 258 | 10 | 32 | 378 | 12 | 381 | 5.78e-11 | 63.9 |
Find 76 sgRNAs with CRISPR-Local
Find 89 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAACACCTATAGCGAAATTT+TGG | 0.201938 | 7.2:+68824684 | MS.gene79178:CDS |
| TCAGATTACAGCTTTGAATT+TGG | 0.229608 | 7.2:+68824472 | MS.gene79178:CDS |
| GGGTCAGAGTTATTTGTTTC+GGG | 0.243484 | 7.2:+68824517 | MS.gene79178:CDS |
| GACAAGAGATTCTATGTTAT+TGG | 0.250445 | 7.2:+68824799 | MS.gene79178:CDS |
| GGTGATGAGTTACTTGTTAT+TGG | 0.263196 | 7.2:+68825102 | MS.gene79178:CDS |
| ATTTGAAAGGGATCAATAAT+TGG | 0.292774 | 7.2:+68825010 | MS.gene79178:CDS |
| CTATTTGCTTCAGCTAGTTC+TGG | 0.294486 | 7.2:+68824628 | MS.gene79178:CDS |
| GCGGCGAGATCTACAAAATA+AGG | 0.302206 | 7.2:+68824338 | MS.gene79178:CDS |
| AGTTTCCAATGTTGTGATCT+TGG | 0.305480 | 7.2:-68824277 | None:intergenic |
| AATTTGACGGACCTGTTCTT+TGG | 0.310795 | 7.2:+68824545 | MS.gene79178:CDS |
| AGCTGATGCACAAGGTGGTT+GGG | 0.325783 | 7.2:+68825059 | MS.gene79178:CDS |
| TTCGCATAAAGAACGGGCTT+TGG | 0.361752 | 7.2:+68825137 | MS.gene79178:CDS |
| CAGAACTAGCTGAAGCAAAT+AGG | 0.366562 | 7.2:-68824627 | None:intergenic |
| GTTGAAAGACATTCCACTTT+CGG | 0.373291 | 7.2:+68824906 | MS.gene79178:CDS |
| TTCAGCTTAATCATTTCATT+AGG | 0.374102 | 7.2:+68825229 | MS.gene79178:CDS |
| CATTTCAATTTCTCACCCTC+AGG | 0.390524 | 7.2:-68825183 | None:intergenic |
| AGGGTCAGAGTTATTTGTTT+CGG | 0.390691 | 7.2:+68824516 | MS.gene79178:CDS |
| TTCTGTTTCGCATAAAGAAC+GGG | 0.392515 | 7.2:+68825131 | MS.gene79178:CDS |
| CTGTAATCTGATTGAATCCT+TGG | 0.399975 | 7.2:-68824460 | None:intergenic |
| TTTCTCTTTGAATTCATCTT+CGG | 0.405173 | 7.2:-68824754 | None:intergenic |
| TGTTCTTTGGCGATATGATT+CGG | 0.417794 | 7.2:+68824558 | MS.gene79178:CDS |
| AATTTCTCACCCTCAGGATC+AGG | 0.424534 | 7.2:-68825177 | None:intergenic |
| TACAAAATAAGGAAAGAGTT+AGG | 0.428564 | 7.2:+68824349 | MS.gene79178:CDS |
| TTTGAATTCATCTTCGGTAA+AGG | 0.447083 | 7.2:-68824748 | None:intergenic |
| TGAAAGCAATTGGTGGGGTA+TGG | 0.450232 | 7.2:+68824408 | MS.gene79178:CDS |
| GGAATGTCTTTCAACATGTC+AGG | 0.452600 | 7.2:-68824898 | None:intergenic |
| CTGAGGGTGAGAAATTGAAA+TGG | 0.473233 | 7.2:+68825184 | MS.gene79178:CDS |
| AGCTTCCTGCTTGATTTGAA+AGG | 0.476416 | 7.2:-68824436 | None:intergenic |
| CGAGCTAGAGACATCGATCT+TGG | 0.476661 | 7.2:+68824246 | MS.gene79178:CDS |
| ATTCTATGTTATTGGTGGAC+AGG | 0.479496 | 7.2:+68824807 | MS.gene79178:CDS |
| GAATCATATCGCCAAAGAAC+AGG | 0.481427 | 7.2:-68824556 | None:intergenic |
| AGCTGAAAGTGTATTTGAAA+GGG | 0.490575 | 7.2:+68824998 | MS.gene79178:CDS |
| AATCAAGCAGGAAGCTTCCA+AGG | 0.494763 | 7.2:+68824443 | MS.gene79178:CDS |
| TAGCTAGCGGTGAAAGCAAT+TGG | 0.495059 | 7.2:+68824398 | MS.gene79178:CDS |
| GAGCTGAAAGTGTATTTGAA+AGG | 0.497856 | 7.2:+68824997 | MS.gene79178:CDS |
| AGTGTCCAAAATTTCGCTAT+AGG | 0.502571 | 7.2:-68824689 | None:intergenic |
| GCTGATGCACAAGGTGGTTG+GGG | 0.505520 | 7.2:+68825060 | MS.gene79178:CDS |
| TAGGAATTGTGCTGTGATGA+TGG | 0.506085 | 7.2:+68825248 | MS.gene79178:CDS |
| GGCGTCGCGTTTAAATCACT+TGG | 0.517536 | 7.2:+68825081 | MS.gene79178:CDS |
| AAATGGAAGCAAATTGATTG+TGG | 0.518481 | 7.2:+68825201 | MS.gene79178:CDS |
| GAAAGAAGAATCTTTACCTC+AGG | 0.525784 | 7.2:+68824195 | MS.gene79178:CDS |
| GTTTCGGGTAAAGAATTTGA+CGG | 0.527787 | 7.2:+68824532 | MS.gene79178:CDS |
| TACTTGTTGTCCTGATCCTG+AGG | 0.533715 | 7.2:+68825167 | MS.gene79178:CDS |
| AAGAGATTCTATGTTATTGG+TGG | 0.534243 | 7.2:+68824802 | MS.gene79178:CDS |
| CCTTGTGCATCAGCTCTCAC+AGG | 0.544876 | 7.2:-68825051 | None:intergenic |
| GATTTCCAAGATCACAACAT+TGG | 0.554912 | 7.2:+68824272 | MS.gene79178:CDS |
| GAGCTGATGCACAAGGTGGT+TGG | 0.555202 | 7.2:+68825058 | MS.gene79178:CDS |
| CTTCTGTTTCGCATAAAGAA+CGG | 0.555565 | 7.2:+68825130 | MS.gene79178:CDS |
| TAGCGGTGAAAGCAATTGGT+GGG | 0.556055 | 7.2:+68824402 | MS.gene79178:CDS |
| ATGATTCGGATACGAATGAA+TGG | 0.557903 | 7.2:+68824572 | MS.gene79178:CDS |
| TGAAGAACCGTTGCCATCAG+AGG | 0.562265 | 7.2:-68824170 | None:intergenic |
| GATACGAATGAATGGTTCAA+AGG | 0.564065 | 7.2:+68824580 | MS.gene79178:CDS |
| GTCCGTTCATGATTAATCCT+AGG | 0.572154 | 7.2:+68824602 | MS.gene79178:CDS |
| GCAATTGGTGGGGTATGGAA+TGG | 0.578887 | 7.2:+68824413 | MS.gene79178:CDS |
| TTACCACCCTCTGATGGCAA+CGG | 0.580464 | 7.2:+68824163 | MS.gene79178:CDS |
| CGTTCTTTATGCGAAACAGA+AGG | 0.590947 | 7.2:-68825129 | None:intergenic |
| GAAGAACCGTTGCCATCAGA+GGG | 0.591725 | 7.2:-68824169 | None:intergenic |
| TATTAACAACAGCTATCAGA+GGG | 0.594157 | 7.2:-68824942 | None:intergenic |
| GGAGAGTGCGAATCCGAAAG+TGG | 0.596088 | 7.2:-68824919 | None:intergenic |
| ACTTGTTGTCCTGATCCTGA+GGG | 0.606724 | 7.2:+68825168 | MS.gene79178:CDS |
| GACTTGTTACCACCCTCTGA+TGG | 0.609485 | 7.2:+68824157 | MS.gene79178:CDS |
| CACCTAGGATTAATCATGAA+CGG | 0.612535 | 7.2:-68824604 | None:intergenic |
| AATGGCCTTTCAAATCAAGC+AGG | 0.615937 | 7.2:+68824431 | MS.gene79178:CDS |
| AGGAATTGTGCTGTGATGAT+GGG | 0.616810 | 7.2:+68825249 | MS.gene79178:CDS |
| CTAGCGGTGAAAGCAATTGG+TGG | 0.633430 | 7.2:+68824401 | MS.gene79178:CDS |
| TTATTAACAACAGCTATCAG+AGG | 0.633695 | 7.2:-68824943 | None:intergenic |
| TCGACGAAGAAACAAACACA+TGG | 0.634887 | 7.2:+68824866 | MS.gene79178:CDS |
| GCTGAAGCAAATAGGCACCT+AGG | 0.638194 | 7.2:-68824619 | None:intergenic |
| AGCGGTGAAAGCAATTGGTG+GGG | 0.655236 | 7.2:+68824403 | MS.gene79178:CDS |
| CCTGTGAGAGCTGATGCACA+AGG | 0.663164 | 7.2:+68825051 | MS.gene79178:CDS |
| AGATTATAATCTGCATCCTG+AGG | 0.665618 | 7.2:-68824211 | None:intergenic |
| GAACCGTTGCCATCAGAGGG+TGG | 0.673262 | 7.2:-68824166 | None:intergenic |
| ATTAACAACAGCTATCAGAG+GGG | 0.676676 | 7.2:-68824941 | None:intergenic |
| TGGACAGGACGAGAATCGAA+AGG | 0.677851 | 7.2:+68824822 | MS.gene79178:CDS |
| TTAACAACAGCTATCAGAGG+GGG | 0.692839 | 7.2:-68824940 | None:intergenic |
| GTGAGAGCTGATGCACAAGG+TGG | 0.739174 | 7.2:+68825054 | MS.gene79178:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATCAATAATTGGAAAAAGTT+AGG | + | chr7.2:68825021-68825040 | MS.gene79178:CDS | 20.0% |
| ! | ATGATTCGAAAACAAAAACT+TGG | + | chr7.2:68824722-68824741 | MS.gene79178:CDS | 25.0% |
| ! | ATTTGAAAGGGATCAATAAT+TGG | + | chr7.2:68825010-68825029 | MS.gene79178:CDS | 25.0% |
| ! | TACAAAATAAGGAAAGAGTT+AGG | + | chr7.2:68824349-68824368 | MS.gene79178:CDS | 25.0% |
| ! | TTCAGCTTAATCATTTCATT+AGG | + | chr7.2:68825229-68825248 | MS.gene79178:CDS | 25.0% |
| ! | TTTCTCTTTGAATTCATCTT+CGG | - | chr7.2:68824757-68824776 | None:intergenic | 25.0% |
| !! | ATAAAGAATCATTTTCAGCA+GGG | + | chr7.2:68824497-68824516 | MS.gene79178:CDS | 25.0% |
| !! | TCAAAGAGAAAATTTTGTTC+AGG | + | chr7.2:68824766-68824785 | MS.gene79178:CDS | 25.0% |
| !!! | TTTTTAACTCTGATGAAAAG+CGG | + | chr7.2:68824319-68824338 | MS.gene79178:CDS | 25.0% |
| AAATGGAAGCAAATTGATTG+TGG | + | chr7.2:68825201-68825220 | MS.gene79178:CDS | 30.0% | |
| AAGAGATTCTATGTTATTGG+TGG | + | chr7.2:68824802-68824821 | MS.gene79178:CDS | 30.0% | |
| GACAAGAGATTCTATGTTAT+TGG | + | chr7.2:68824799-68824818 | MS.gene79178:CDS | 30.0% | |
| TATTAACAACAGCTATCAGA+GGG | - | chr7.2:68824945-68824964 | None:intergenic | 30.0% | |
| TTATTAACAACAGCTATCAG+AGG | - | chr7.2:68824946-68824965 | None:intergenic | 30.0% | |
| TTTGAATTCATCTTCGGTAA+AGG | - | chr7.2:68824751-68824770 | None:intergenic | 30.0% | |
| ! | AAACACCTATAGCGAAATTT+TGG | + | chr7.2:68824684-68824703 | MS.gene79178:CDS | 30.0% |
| ! | AGCTGAAAGTGTATTTGAAA+GGG | + | chr7.2:68824998-68825017 | MS.gene79178:CDS | 30.0% |
| ! | ATTTTGTTCAGGATGTTACA+TGG | + | chr7.2:68824777-68824796 | MS.gene79178:CDS | 30.0% |
| ! | GATAAAGAATCATTTTCAGC+AGG | + | chr7.2:68824496-68824515 | MS.gene79178:CDS | 30.0% |
| !! | TCAGATTACAGCTTTGAATT+TGG | + | chr7.2:68824472-68824491 | MS.gene79178:CDS | 30.0% |
| ACCGCTAGCTAACATAAAAA+CGG | - | chr7.2:68824389-68824408 | None:intergenic | 35.0% | |
| AGATTATAATCTGCATCCTG+AGG | - | chr7.2:68824214-68824233 | None:intergenic | 35.0% | |
| AGGGTCAGAGTTATTTGTTT+CGG | + | chr7.2:68824516-68824535 | MS.gene79178:CDS | 35.0% | |
| AGTGTCCAAAATTTCGCTAT+AGG | - | chr7.2:68824692-68824711 | None:intergenic | 35.0% | |
| AGTTTCCAATGTTGTGATCT+TGG | - | chr7.2:68824280-68824299 | None:intergenic | 35.0% | |
| ATTAACAACAGCTATCAGAG+GGG | - | chr7.2:68824944-68824963 | None:intergenic | 35.0% | |
| CACCTAGGATTAATCATGAA+CGG | - | chr7.2:68824607-68824626 | None:intergenic | 35.0% | |
| CTAGCTAACATAAAAACGGA+CGG | - | chr7.2:68824385-68824404 | None:intergenic | 35.0% | |
| CTGTAATCTGATTGAATCCT+TGG | - | chr7.2:68824463-68824482 | None:intergenic | 35.0% | |
| CTTCTGTTTCGCATAAAGAA+CGG | + | chr7.2:68825130-68825149 | MS.gene79178:CDS | 35.0% | |
| GAAAGAAGAATCTTTACCTC+AGG | + | chr7.2:68824195-68824214 | MS.gene79178:CDS | 35.0% | |
| GATACGAATGAATGGTTCAA+AGG | + | chr7.2:68824580-68824599 | MS.gene79178:CDS | 35.0% | |
| GATTTCCAAGATCACAACAT+TGG | + | chr7.2:68824272-68824291 | MS.gene79178:CDS | 35.0% | |
| GTTGAAAGACATTCCACTTT+CGG | + | chr7.2:68824906-68824925 | MS.gene79178:CDS | 35.0% | |
| TGTTCTTTGGCGATATGATT+CGG | + | chr7.2:68824558-68824577 | MS.gene79178:CDS | 35.0% | |
| TTCTGTTTCGCATAAAGAAC+GGG | + | chr7.2:68825131-68825150 | MS.gene79178:CDS | 35.0% | |
| ! | ATGATTCGGATACGAATGAA+TGG | + | chr7.2:68824572-68824591 | MS.gene79178:CDS | 35.0% |
| ! | ATTCTATGTTATTGGTGGAC+AGG | + | chr7.2:68824807-68824826 | MS.gene79178:CDS | 35.0% |
| ! | GAGCTGAAAGTGTATTTGAA+AGG | + | chr7.2:68824997-68825016 | MS.gene79178:CDS | 35.0% |
| ! | GTTTCGGGTAAAGAATTTGA+CGG | + | chr7.2:68824532-68824551 | MS.gene79178:CDS | 35.0% |
| !! | AATTTTGCTTTTGTTGCAGG+TGG | + | chr7.2:68824652-68824671 | MS.gene79178:CDS | 35.0% |
| !! | GGTAATTTTGCTTTTGTTGC+AGG | + | chr7.2:68824649-68824668 | MS.gene79178:CDS | 35.0% |
| !! | GGTGATGAGTTACTTGTTAT+TGG | + | chr7.2:68825102-68825121 | MS.gene79178:CDS | 35.0% |
| !! | TCCGTTTTTATGTTAGCTAG+CGG | + | chr7.2:68824385-68824404 | MS.gene79178:CDS | 35.0% |
| AATGGCCTTTCAAATCAAGC+AGG | + | chr7.2:68824431-68824450 | MS.gene79178:CDS | 40.0% | |
| AGGAATTGTGCTGTGATGAT+GGG | + | chr7.2:68825249-68825268 | MS.gene79178:CDS | 40.0% | |
| CAGAACTAGCTGAAGCAAAT+AGG | - | chr7.2:68824630-68824649 | None:intergenic | 40.0% | |
| CATTTCAATTTCTCACCCTC+AGG | - | chr7.2:68825186-68825205 | None:intergenic | 40.0% | |
| CGTTCTTTATGCGAAACAGA+AGG | - | chr7.2:68825132-68825151 | None:intergenic | 40.0% | |
| CTATTTGCTTCAGCTAGTTC+TGG | + | chr7.2:68824628-68824647 | MS.gene79178:CDS | 40.0% | |
| CTGAGGGTGAGAAATTGAAA+TGG | + | chr7.2:68825184-68825203 | MS.gene79178:CDS | 40.0% | |
| GAATCATATCGCCAAAGAAC+AGG | - | chr7.2:68824559-68824578 | None:intergenic | 40.0% | |
| GGAATGTCTTTCAACATGTC+AGG | - | chr7.2:68824901-68824920 | None:intergenic | 40.0% | |
| GGGTCAGAGTTATTTGTTTC+GGG | + | chr7.2:68824517-68824536 | MS.gene79178:CDS | 40.0% | |
| GTCCGTTCATGATTAATCCT+AGG | + | chr7.2:68824602-68824621 | MS.gene79178:CDS | 40.0% | |
| TAGGAATTGTGCTGTGATGA+TGG | + | chr7.2:68825248-68825267 | MS.gene79178:CDS | 40.0% | |
| TCGACGAAGAAACAAACACA+TGG | + | chr7.2:68824866-68824885 | MS.gene79178:CDS | 40.0% | |
| TTAACAACAGCTATCAGAGG+GGG | - | chr7.2:68824943-68824962 | None:intergenic | 40.0% | |
| ! | AATTTGACGGACCTGTTCTT+TGG | + | chr7.2:68824545-68824564 | MS.gene79178:CDS | 40.0% |
| ! | AGCTTCCTGCTTGATTTGAA+AGG | - | chr7.2:68824439-68824458 | None:intergenic | 40.0% |
| AATCAAGCAGGAAGCTTCCA+AGG | + | chr7.2:68824443-68824462 | MS.gene79178:CDS | 45.0% | |
| AATTTCTCACCCTCAGGATC+AGG | - | chr7.2:68825180-68825199 | None:intergenic | 45.0% | |
| GCGGCGAGATCTACAAAATA+AGG | + | chr7.2:68824338-68824357 | MS.gene79178:CDS | 45.0% | |
| TAGCGGTGAAAGCAATTGGT+GGG | + | chr7.2:68824402-68824421 | MS.gene79178:CDS | 45.0% | |
| TAGCTAGCGGTGAAAGCAAT+TGG | + | chr7.2:68824398-68824417 | MS.gene79178:CDS | 45.0% | |
| TGAAAGCAATTGGTGGGGTA+TGG | + | chr7.2:68824408-68824427 | MS.gene79178:CDS | 45.0% | |
| ! | TGCTTTTGTTGCAGGTGGTT+TGG | + | chr7.2:68824657-68824676 | MS.gene79178:CDS | 45.0% |
| ! | TTCGCATAAAGAACGGGCTT+TGG | + | chr7.2:68825137-68825156 | MS.gene79178:CDS | 45.0% |
| !! | ACTTGTTGTCCTGATCCTGA+GGG | + | chr7.2:68825168-68825187 | MS.gene79178:CDS | 45.0% |
| !! | TACTTGTTGTCCTGATCCTG+AGG | + | chr7.2:68825167-68825186 | MS.gene79178:CDS | 45.0% |
| AGCGGTGAAAGCAATTGGTG+GGG | + | chr7.2:68824403-68824422 | MS.gene79178:CDS | 50.0% | |
| AGCTGATGCACAAGGTGGTT+GGG | + | chr7.2:68825059-68825078 | MS.gene79178:CDS | 50.0% | |
| CGAGCTAGAGACATCGATCT+TGG | + | chr7.2:68824246-68824265 | MS.gene79178:CDS | 50.0% | |
| CTAGCGGTGAAAGCAATTGG+TGG | + | chr7.2:68824401-68824420 | MS.gene79178:CDS | 50.0% | |
| GAAGAACCGTTGCCATCAGA+GGG | - | chr7.2:68824172-68824191 | None:intergenic | 50.0% | |
| GCAATTGGTGGGGTATGGAA+TGG | + | chr7.2:68824413-68824432 | MS.gene79178:CDS | 50.0% | |
| GCTGAAGCAAATAGGCACCT+AGG | - | chr7.2:68824622-68824641 | None:intergenic | 50.0% | |
| GGCGTCGCGTTTAAATCACT+TGG | + | chr7.2:68825081-68825100 | MS.gene79178:CDS | 50.0% | |
| TGAAGAACCGTTGCCATCAG+AGG | - | chr7.2:68824173-68824192 | None:intergenic | 50.0% | |
| TGGACAGGACGAGAATCGAA+AGG | + | chr7.2:68824822-68824841 | MS.gene79178:CDS | 50.0% | |
| TTACCACCCTCTGATGGCAA+CGG | + | chr7.2:68824163-68824182 | MS.gene79178:CDS | 50.0% | |
| !! | GACTTGTTACCACCCTCTGA+TGG | + | chr7.2:68824157-68824176 | MS.gene79178:CDS | 50.0% |
| CCTGTGAGAGCTGATGCACA+AGG | + | chr7.2:68825051-68825070 | MS.gene79178:CDS | 55.0% | |
| CCTTGTGCATCAGCTCTCAC+AGG | - | chr7.2:68825054-68825073 | None:intergenic | 55.0% | |
| GAGCTGATGCACAAGGTGGT+TGG | + | chr7.2:68825058-68825077 | MS.gene79178:CDS | 55.0% | |
| GCTGATGCACAAGGTGGTTG+GGG | + | chr7.2:68825060-68825079 | MS.gene79178:CDS | 55.0% | |
| GTGAGAGCTGATGCACAAGG+TGG | + | chr7.2:68825054-68825073 | MS.gene79178:CDS | 55.0% | |
| ! | GGAGAGTGCGAATCCGAAAG+TGG | - | chr7.2:68824922-68824941 | None:intergenic | 55.0% |
| GAACCGTTGCCATCAGAGGG+TGG | - | chr7.2:68824169-68824188 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 68824145 | 68825281 | 68824145 | ID=MS.gene79178 |
| chr7.2 | mRNA | 68824145 | 68825281 | 68824145 | ID=MS.gene79178.t1;Parent=MS.gene79178 |
| chr7.2 | exon | 68824145 | 68825281 | 68824145 | ID=MS.gene79178.t1.exon1;Parent=MS.gene79178.t1 |
| chr7.2 | CDS | 68824145 | 68825281 | 68824145 | ID=cds.MS.gene79178.t1;Parent=MS.gene79178.t1 |
| Gene Sequence |
| Protein sequence |
