AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene83495


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene83495.t1 MTR_5g025520 97.744 133 3 0 1 133 1 133 3.30e-93 265
MS.gene83495.t1 MTR_5g025500 37.662 77 48 0 26 102 54 130 1.42e-11 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 46 sgRNAs with CRISPR-Local

Find 102 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCATCAAACTCTACAGTTTC+CGG 0.200149 5.3:-15076787 MS.gene83495:CDS
ATGAATCCGGCGGGTTTAAC+AGG 0.280966 5.3:-15076959 MS.gene83495:CDS
GGAAATGATGGAAGCAATTT+CGG 0.295914 5.3:+15076814 None:intergenic
CATGATTTCGGCGACGGCTT+TGG 0.360532 5.3:+15076913 None:intergenic
CAGTGAACTCTGCACTTTCA+TGG 0.368977 5.3:-15076858 MS.gene83495:CDS
CATCAAACTCTACAGTTTCC+GGG 0.376717 5.3:-15076786 MS.gene83495:intron
ATCGCACCCTGGCTGAATTC+CGG 0.377231 5.3:+15075618 None:intergenic
TTTGCCGACACCCATGATTT+CGG 0.381159 5.3:+15076901 None:intergenic
TTTGATGAACTTGGAAATGA+TGG 0.402949 5.3:+15076802 None:intergenic
ACTGGTAGCTTTGCGTGTCT+TGG 0.404946 5.3:+15076877 None:intergenic
CAAAGCCGTCGCCGAAATCA+TGG 0.409452 5.3:-15076912 MS.gene83495:CDS
GTATCAAGAAAGACAAGCTT+TGG 0.428352 5.3:-15075713 MS.gene83495:CDS
ACTGTAGAGTTTGATGAACT+TGG 0.446589 5.3:+15076793 None:intergenic
GGGAAAGGCCAACAGAAGAA+AGG 0.464992 5.3:-15075549 MS.gene83495:CDS
GACAGAATCTTAGCAATCTC+AGG 0.466374 5.3:+15075642 None:intergenic
TAATATCAAGGATACTAATA+CGG 0.503603 5.3:-15075595 MS.gene83495:CDS
TCTGTCTCCGGAATTCAGCC+AGG 0.504471 5.3:-15075625 MS.gene83495:CDS
GACACCCATGATTTCGGCGA+CGG 0.507275 5.3:+15076907 None:intergenic
GGAAAGGCCAACAGAAGAAA+GGG 0.508550 5.3:-15075548 MS.gene83495:CDS
GATTGCTAAGATTCTGTCTC+CGG 0.511219 5.3:-15075637 MS.gene83495:CDS
AGTGAACTCTGCACTTTCAT+GGG 0.512210 5.3:-15076857 MS.gene83495:CDS
AGCTTGTCTTTCTTGATACC+AGG 0.515565 5.3:+15075717 None:intergenic
TATGGTGTTGTGCAGAGTCC+TGG 0.516143 5.3:-15075735 MS.gene83495:intron
CCAGGGTGCGATTAATATCA+AGG 0.519238 5.3:-15075607 MS.gene83495:CDS
CATGAAAGTGCAGAGTTCAC+TGG 0.524418 5.3:+15076859 None:intergenic
ATCCAGCAGAAGGATGAATC+CGG 0.526821 5.3:-15076972 None:intergenic
CTTCGCCCTGTTAAACCCGC+CGG 0.548257 5.3:+15076953 None:intergenic
TATCAAGAAAGACAAGCTTT+GGG 0.550356 5.3:-15075712 MS.gene83495:CDS
TCTTCCGCGGATAACACAAA+GGG 0.561908 5.3:-15075570 MS.gene83495:CDS
AAAGCCGTCGCCGAAATCAT+GGG 0.576114 5.3:-15076911 MS.gene83495:CDS
GCGGATAACACAAAGGGGAA+AGG 0.582569 5.3:-15075564 MS.gene83495:CDS
TTCTTCCGCGGATAACACAA+AGG 0.602214 5.3:-15075571 MS.gene83495:CDS
CGGCGGGTTTAACAGGGCGA+AGG 0.602549 5.3:-15076952 MS.gene83495:CDS
TGAATCCGGCGGGTTTAACA+GGG 0.604481 5.3:-15076958 MS.gene83495:CDS
GTCGCCGAAATCATGGGTGT+CGG 0.610089 5.3:-15076905 MS.gene83495:CDS
AGCAGAAGGATGAATCCGGC+GGG 0.612519 5.3:-15076968 None:intergenic
AATTTGCAAACACTGTTGCG+TGG 0.632781 5.3:-15075684 MS.gene83495:CDS
TTGCGTGGCAGAAACAGCGT+TGG 0.639499 5.3:-15075669 MS.gene83495:CDS
ATTTCGGAACGAGATTGATG+AGG 0.642540 5.3:+15076830 None:intergenic
CTGTCTCCGGAATTCAGCCA+GGG 0.642587 5.3:-15075624 MS.gene83495:CDS
AGGGCGAAGGAGCATGTCGA+CGG 0.655015 5.3:-15076939 MS.gene83495:CDS
CAGCAGAAGGATGAATCCGG+CGG 0.656372 5.3:-15076969 None:intergenic
TACTAATACGGATTCTTCCG+CGG 0.657291 5.3:-15075583 MS.gene83495:CDS
CCTTGATATTAATCGCACCC+TGG 0.662781 5.3:+15075607 None:intergenic
TTTCCCCTTTGTGTTATCCG+CGG 0.666626 5.3:+15075566 None:intergenic
CTTCCGCGGATAACACAAAG+GGG 0.722706 5.3:-15075569 MS.gene83495:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTATAATAAAAGATAAAATA+AGG + chr5.3:15076580-15076599 None:intergenic 10.0%
!! AATGAAACAAAAAGAAAAAT+GGG + chr5.3:15075746-15075765 None:intergenic 15.0%
!! TAATGAAACAAAAAGAAAAA+TGG + chr5.3:15075747-15075766 None:intergenic 15.0%
!!! TTTATTTACTTTTGCTTATT+TGG - chr5.3:15076729-15076748 MS.gene83495:intron 15.0%
!! ATTAAGCTGTTTATAAAAAC+AGG - chr5.3:15076523-15076542 MS.gene83495:intron 20.0%
!! CTTTCCATAAAATAAGATAT+AGG + chr5.3:15076550-15076569 None:intergenic 20.0%
!! TAATATCAAGGATACTAATA+CGG - chr5.3:15076900-15076919 MS.gene83495:CDS 20.0%
!! TCAAATAAACCAATTCAAAT+AGG + chr5.3:15076114-15076133 None:intergenic 20.0%
!! TTAAGCTGTTTATAAAAACA+GGG - chr5.3:15076524-15076543 MS.gene83495:intron 20.0%
!! TTTCAATACATGGATAAAAA+GGG + chr5.3:15076228-15076247 None:intergenic 20.0%
!!! TAAGTTTGTTTCTATAACAA+AGG + chr5.3:15076489-15076508 None:intergenic 20.0%
! AAGATTAAAGACTAATCAGT+AGG + chr5.3:15075987-15076006 None:intergenic 25.0%
! AGATTAAAGACTAATCAGTA+GGG + chr5.3:15075986-15076005 None:intergenic 25.0%
! GTTTCAATACATGGATAAAA+AGG + chr5.3:15076229-15076248 None:intergenic 25.0%
! TTGAAAATAATCACTAGTCA+GGG + chr5.3:15076073-15076092 None:intergenic 25.0%
! TTTGAAAATAATCACTAGTC+AGG + chr5.3:15076074-15076093 None:intergenic 25.0%
!! TAAACTATCTCTGAAAAACA+TGG + chr5.3:15075823-15075842 None:intergenic 25.0%
!! TACTTTTGCTTATTTGGAAA+TGG - chr5.3:15076735-15076754 MS.gene83495:intron 25.0%
ATTAAAGACTAATCAGTAGG+GGG + chr5.3:15075984-15076003 None:intergenic 30.0%
GATTAAAGACTAATCAGTAG+GGG + chr5.3:15075985-15076004 None:intergenic 30.0%
GCTTATTTGGAAATGGTATA+TGG - chr5.3:15076742-15076761 MS.gene83495:intron 30.0%
TATCAAGAAAGACAAGCTTT+GGG - chr5.3:15076783-15076802 MS.gene83495:intron 30.0%
TCTGAAAAACATGGAAATGA+TGG + chr5.3:15075814-15075833 None:intergenic 30.0%
TTGAGAGAACTTATGAAAAC+AGG - chr5.3:15076354-15076373 MS.gene83495:intron 30.0%
TTTGATGAACTTGGAAATGA+TGG + chr5.3:15075696-15075715 None:intergenic 30.0%
!!! AGGGCCTATATCTTATTTTA+TGG - chr5.3:15076543-15076562 MS.gene83495:intron 30.0%
AAATGGGAAGAATTGAAACC+CGG + chr5.3:15075730-15075749 None:intergenic 35.0%
AACCAATTCAAATAGGCTCA+TGG + chr5.3:15076107-15076126 None:intergenic 35.0%
AACCATGAGCCTATTTGAAT+TGG - chr5.3:15076102-15076121 MS.gene83495:intron 35.0%
ACTGTAGAGTTTGATGAACT+TGG + chr5.3:15075705-15075724 None:intergenic 35.0%
CAAGTGCTTGTTTCAATACA+TGG + chr5.3:15076238-15076257 None:intergenic 35.0%
GAGAACATTGATCAAACAGT+TGG + chr5.3:15075777-15075796 None:intergenic 35.0%
GGATAAACAACTTCATTGAG+TGG + chr5.3:15076654-15076673 None:intergenic 35.0%
GTATCAAGAAAGACAAGCTT+TGG - chr5.3:15076782-15076801 MS.gene83495:intron 35.0%
TACAATACACCAACAATGGT+GGG - chr5.3:15076154-15076173 MS.gene83495:intron 35.0%
TCATCAAACTCTACAGTTTC+CGG - chr5.3:15075708-15075727 MS.gene83495:CDS 35.0%
TTATTTGAGCTCATATACCC+TGG - chr5.3:15076126-15076145 MS.gene83495:intron 35.0%
! AGCGATAAGCTCTTTTCATA+AGG + chr5.3:15076437-15076456 None:intergenic 35.0%
! GGAAATGATGGAAGCAATTT+CGG + chr5.3:15075684-15075703 None:intergenic 35.0%
! TCTTTTTCCCTTTCTTCTGT+TGG + chr5.3:15076957-15076976 None:intergenic 35.0%
AATTTGCAAACACTGTTGCG+TGG - chr5.3:15076811-15076830 MS.gene83495:CDS 40.0%
AGCTTGTCTTTCTTGATACC+AGG + chr5.3:15076781-15076800 None:intergenic 40.0%
AGTCCCTATCAGTGATTAAG+TGG + chr5.3:15076029-15076048 None:intergenic 40.0%
AGTGAACTCTGCACTTTCAT+GGG - chr5.3:15075638-15075657 MS.gene83495:CDS 40.0%
ATTTCGGAACGAGATTGATG+AGG + chr5.3:15075668-15075687 None:intergenic 40.0%
CAATGGCAACACTCTTACTT+AGG + chr5.3:15076684-15076703 None:intergenic 40.0%
CACCCACTTAATCACTGATA+GGG - chr5.3:15076023-15076042 MS.gene83495:intron 40.0%
CACTCTTACTTAGGCTCATT+TGG + chr5.3:15076675-15076694 None:intergenic 40.0%
CATCAAACTCTACAGTTTCC+GGG - chr5.3:15075709-15075728 MS.gene83495:CDS 40.0%
CTTTCACTTTAGTCGCTCAA+TGG + chr5.3:15076701-15076720 None:intergenic 40.0%
GACAGAATCTTAGCAATCTC+AGG + chr5.3:15076856-15076875 None:intergenic 40.0%
GATTGCTAAGATTCTGTCTC+CGG - chr5.3:15076858-15076877 MS.gene83495:CDS 40.0%
GTACAATACACCAACAATGG+TGG - chr5.3:15076153-15076172 MS.gene83495:intron 40.0%
GTCCCTATCAGTGATTAAGT+GGG + chr5.3:15076028-15076047 None:intergenic 40.0%
TACTAATACGGATTCTTCCG+CGG - chr5.3:15076912-15076931 MS.gene83495:CDS 40.0%
TCACCCACTTAATCACTGAT+AGG - chr5.3:15076022-15076041 MS.gene83495:intron 40.0%
TGCGTACAATACACCAACAA+TGG - chr5.3:15076150-15076169 MS.gene83495:intron 40.0%
TTGAGTGGTTATAGCACAAG+CGG + chr5.3:15076639-15076658 None:intergenic 40.0%
CAGTGAACTCTGCACTTTCA+TGG - chr5.3:15075637-15075656 MS.gene83495:CDS 45.0%
CATGGATAAAAAGGGCAACC+CGG + chr5.3:15076220-15076239 None:intergenic 45.0%
CCAGGGTGCGATTAATATCA+AGG - chr5.3:15076888-15076907 MS.gene83495:CDS 45.0%
CCTTGATATTAATCGCACCC+TGG + chr5.3:15076891-15076910 None:intergenic 45.0%
GGAAAGGCCAACAGAAGAAA+GGG - chr5.3:15076947-15076966 MS.gene83495:CDS 45.0%
TCTTCCGCGGATAACACAAA+GGG - chr5.3:15076925-15076944 MS.gene83495:CDS 45.0%
TTCTTCCGCGGATAACACAA+AGG - chr5.3:15076924-15076943 MS.gene83495:CDS 45.0%
TTTCCCCTTTGTGTTATCCG+CGG + chr5.3:15076932-15076951 None:intergenic 45.0%
TTTGCCGACACCCATGATTT+CGG + chr5.3:15075597-15075616 None:intergenic 45.0%
! CATGAAAGTGCAGAGTTCAC+TGG + chr5.3:15075639-15075658 None:intergenic 45.0%
AAAGCCGTCGCCGAAATCAT+GGG - chr5.3:15075584-15075603 MS.gene83495:CDS 50.0%
ACTAAAGCTCCCGCATATGC+AGG + chr5.3:15076194-15076213 None:intergenic 50.0%
CTAAAGCTCCCGCATATGCA+GGG + chr5.3:15076193-15076212 None:intergenic 50.0%
CTTCCGCGGATAACACAAAG+GGG - chr5.3:15076926-15076945 MS.gene83495:CDS 50.0%
GCGGATAACACAAAGGGGAA+AGG - chr5.3:15076931-15076950 MS.gene83495:CDS 50.0%
GGGAAAGGCCAACAGAAGAA+AGG - chr5.3:15076946-15076965 MS.gene83495:CDS 50.0%
TGGTGTATTGTACGCAGCCA+GGG + chr5.3:15076146-15076165 None:intergenic 50.0%
TTGGTGTATTGTACGCAGCC+AGG + chr5.3:15076147-15076166 None:intergenic 50.0%
! ACTGGTAGCTTTGCGTGTCT+TGG + chr5.3:15075621-15075640 None:intergenic 50.0%
! ATGAATCCGGCGGGTTTAAC+AGG - chr5.3:15075536-15075555 MS.gene83495:CDS 50.0%
! TGAATCCGGCGGGTTTAACA+GGG - chr5.3:15075537-15075556 MS.gene83495:CDS 50.0%
!! TATGGTGTTGTGCAGAGTCC+TGG - chr5.3:15076760-15076779 MS.gene83495:intron 50.0%
ATCGCACCCTGGCTGAATTC+CGG + chr5.3:15076880-15076899 None:intergenic 55.0%
CAAAGCCGTCGCCGAAATCA+TGG - chr5.3:15075583-15075602 MS.gene83495:CDS 55.0%
CATGATTTCGGCGACGGCTT+TGG + chr5.3:15075585-15075604 None:intergenic 55.0%
CTGTCTCCGGAATTCAGCCA+GGG - chr5.3:15076871-15076890 MS.gene83495:CDS 55.0%
GACACCCATGATTTCGGCGA+CGG + chr5.3:15075591-15075610 None:intergenic 55.0%
GTCGCCGAAATCATGGGTGT+CGG - chr5.3:15075590-15075609 MS.gene83495:CDS 55.0%
TCTGTCTCCGGAATTCAGCC+AGG - chr5.3:15076870-15076889 MS.gene83495:CDS 55.0%
TTGCGTGGCAGAAACAGCGT+TGG - chr5.3:15076826-15076845 MS.gene83495:CDS 55.0%
! ATGCGGGAGCTTTAGTGCAC+CGG - chr5.3:15076198-15076217 MS.gene83495:intron 55.0%
CATATGCAGGGTCCGGGAAG+GGG + chr5.3:15076181-15076200 None:intergenic 60.0%
CTTCCCGGACCCTGCATATG+CGG - chr5.3:15076181-15076200 MS.gene83495:intron 60.0%
CTTCGCCCTGTTAAACCCGC+CGG + chr5.3:15075545-15075564 None:intergenic 60.0%
GCATATGCAGGGTCCGGGAA+GGG + chr5.3:15076182-15076201 None:intergenic 60.0%
GGAAGGGGTCCCACCATTGT+TGG + chr5.3:15076166-15076185 None:intergenic 60.0%
TTCCCGGACCCTGCATATGC+GGG - chr5.3:15076182-15076201 MS.gene83495:intron 60.0%
! ACAATGGTGGGACCCCTTCC+CGG - chr5.3:15076166-15076185 MS.gene83495:intron 60.0%
! AGGGCGAAGGAGCATGTCGA+CGG - chr5.3:15075556-15075575 MS.gene83495:CDS 60.0%
! TGCGGGAGCTTTAGTGCACC+GGG - chr5.3:15076199-15076218 MS.gene83495:intron 60.0%
CGCATATGCAGGGTCCGGGA+AGG + chr5.3:15076183-15076202 None:intergenic 65.0%
CTCCCGCATATGCAGGGTCC+GGG + chr5.3:15076187-15076206 None:intergenic 65.0%
GCTCCCGCATATGCAGGGTC+CGG + chr5.3:15076188-15076207 None:intergenic 65.0%
! CGGCGGGTTTAACAGGGCGA+AGG - chr5.3:15075543-15075562 MS.gene83495:CDS 65.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 15075536 15076981 15075536 ID=MS.gene83495
chr5.3 mRNA 15075536 15076981 15075536 ID=MS.gene83495.t1;Parent=MS.gene83495
chr5.3 exon 15076787 15076981 15076787 ID=MS.gene83495.t1.exon1;Parent=MS.gene83495.t1
chr5.3 CDS 15076787 15076981 15076787 ID=cds.MS.gene83495.t1;Parent=MS.gene83495.t1
chr5.3 exon 15075536 15075742 15075536 ID=MS.gene83495.t1.exon2;Parent=MS.gene83495.t1
chr5.3 CDS 15075536 15075742 15075536 ID=cds.MS.gene83495.t1;Parent=MS.gene83495.t1
Gene Sequence

>MS.gene83495

ATGAATCCGGCGGGTTTAACAGGGCGAAGGAGCATGTCGACGGTATCCAAAGCCGTCGCCGAAATCATGGGTGTCGGCAAAACCAAGACACGCAAAGCTACCAGTGAACTCTGCACTTTCATGGGCATTCCTCATCAATCTCGTTCCGAAATTGCTTCCATCATTTCCAAGTTCATCAAACTCTACAGTTTCCGGGTTTCAATTCTTCCCATTTTTCTTTTTGTTTCATTATTGCATACCAACTGTTTGATCAATGTTCTCACTCACAAAACATGCCATCATTTCCATGTTTTTCAGAGATAGTTTATGAAAATAGCTTAAACTTAACACGAAAACAATTTAACTTTATTTTATTTTTGTTATAGAAATAGCTTAGCAGTTAGCAGCTATAAGCTGCAAATTATTATTATACTATGACACTCAATTTATTTGTTCAAGTTTAACTCCCCCTACTGATTAGTCTTTAATCTTTACATATATGATAACTCACCCACTTAATCACTGATAGGGACTAACGAGATGAATTTTCATATACCCTGACTAGTGATTATTTTCAAATATTTTAGAACCATGAGCCTATTTGAATTGGTTTATTTGAGCTCATATACCCTGGCTGCGTACAATACACCAACAATGGTGGGACCCCTTCCCGGACCCTGCATATGCGGGAGCTTTAGTGCACCGGGTTGCCCTTTTTATCCATGTATTGAAACAAGCACTTGTGAGACTGTTTGAGAGAACATATAGAAGCAACACATGACATAAGAAATTATGACTTATTTGAGCTTATCTCTTGACATAAGAACTTGTGAGACTGTTTGAGAGAACTTATGAAAACAGGTTATAGCACATTATTCATATGCTGTTTTCAGCTTGTTTGCGTAAGCTCTTAAAAAAACCTTATGAAAAGAGCTTATCGCTTATACTAAAACAATTCAACTTTATTTTATCCTTTGTTATAGAAACAAACTTATACATAAGCTGCAAATTAAGCTGTTTATAAAAACAGGGCCTATATCTTATTTTATGGAAAGAATTTGACCTTATTTTATCTTTTATTATAGAAATAGCTTATATATACATAAATACTTATGTGAGAACCGCTTGTGCTATAACCACTCAATGAAGTTGTTTATCCAAATGAGCCTAAGTAAGAGTGTTGCCATTGAGCGACTAAAGTGAAAGTTTACTGTTTTATTTACTTTTGCTTATTTGGAAATGGTATATGGTGTTGTGCAGAGTCCTGGTATCAAGAAAGACAAGCTTTGGGAGCAGAATTTGCAAACACTGTTGCGTGGCAGAAACAGCGTTGGTTTTCCTGAGATTGCTAAGATTCTGTCTCCGGAATTCAGCCAGGGTGCGATTAATATCAAGGATACTAATACGGATTCTTCCGCGGATAACACAAAGGGGAAAGGCCAACAGAAGAAAGGGAAAAAGAAATAG

Protein sequence

>MS.gene83495.t1

MNPAGLTGRRSMSTVSKAVAEIMGVGKTKTRKATSELCTFMGIPHQSRSEIASIISKFIKLYSFRSPGIKKDKLWEQNLQTLLRGRNSVGFPEIAKILSPEFSQGAINIKDTNTDSSADNTKGKGQQKKGKKK