AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene88062


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene88062.t1 MTR_7g028160 95.946 222 5 2 1 221 66 284 5.05e-152 423
MS.gene88062.t1 MTR_5g039600 57.292 96 24 3 1 79 83 178 3.67e-25 102
MS.gene88062.t1 MTR_2g006150 75.000 64 10 1 1 58 146 209 1.69e-24 100
MS.gene88062.t1 MTR_2g006150 75.000 64 10 1 1 58 146 209 1.83e-24 100
MS.gene88062.t1 MTR_8g033070 70.968 62 18 0 1 62 96 157 2.37e-24 98.6
MS.gene88062.t1 MTR_4g108370 89.362 47 5 0 1 47 113 159 8.89e-24 98.6
MS.gene88062.t1 MTR_1g101810 70.968 62 18 0 1 62 80 141 1.11e-23 97.1
MS.gene88062.t1 MTR_1g063870 85.106 47 7 0 1 47 65 111 1.60e-23 94.0
MS.gene88062.t1 MTR_1g101810 70.968 62 18 0 1 62 110 171 1.71e-23 97.1
MS.gene88062.t1 MTR_1g038650 62.162 74 28 0 1 74 48 121 4.25e-23 93.6
MS.gene88062.t1 MTR_1g114380 83.333 48 8 0 1 48 49 96 2.59e-22 92.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene88062.t1 AT3G27010 56.061 198 64 7 1 192 94 274 2.95e-49 163
MS.gene88062.t1 AT3G27010 56.061 198 64 7 1 192 94 274 2.95e-49 163
MS.gene88062.t1 AT1G58100 75.000 64 10 1 1 58 76 139 2.56e-25 102
MS.gene88062.t1 AT5G41030 35.437 206 69 11 1 204 84 227 9.82e-25 98.2
MS.gene88062.t1 AT2G45680 64.706 68 24 0 1 68 91 158 4.58e-24 98.2
MS.gene88062.t1 AT1G69690 85.417 48 7 0 1 48 68 115 6.06e-24 97.4
MS.gene88062.t1 AT3G47620 91.489 47 4 0 1 47 133 179 6.42e-24 99.4
MS.gene88062.t1 AT1G35560 73.438 64 11 1 1 58 71 134 7.68e-24 97.4
MS.gene88062.t1 AT1G72010 87.234 47 6 0 1 47 79 125 1.96e-23 97.1
MS.gene88062.t1 AT5G51910 65.625 64 22 0 1 64 73 136 2.55e-23 95.5
MS.gene88062.t1 AT5G51910 65.625 64 22 0 1 64 73 136 2.55e-23 95.5
MS.gene88062.t1 AT5G08330 72.881 59 16 0 1 59 47 105 2.89e-23 94.0
MS.gene88062.t1 AT5G23280 83.333 48 8 0 1 48 55 102 1.40e-22 92.4
MS.gene88062.t1 AT2G37000 84.091 44 7 0 1 44 59 102 4.53e-19 81.6
MS.gene88062.t1 AT3G45150 58.140 43 18 0 1 43 33 75 1.10e-11 61.6

Find 55 sgRNAs with CRISPR-Local

Find 63 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGATGCCTGGTTTGGAATT+AGG 0.117190 7.1:+69470722 MS.gene88062:CDS
ATGTTAGTTCTACAAGTTTA+TGG 0.160920 7.1:+69470504 MS.gene88062:CDS
CAAGGTTCATCTTTGTCTTC+TGG 0.222188 7.1:+69470404 MS.gene88062:CDS
AGTTGAATGATAGGAATAAT+TGG 0.236296 7.1:+69470432 MS.gene88062:CDS
CATCATCAAGTTGGTGGATA+TGG 0.242402 7.1:+69470530 MS.gene88062:CDS
TATGCCAACAGGAACAAATT+TGG 0.247795 7.1:+69470652 MS.gene88062:CDS
GAGTATTATTCTGCTGATTC+TGG 0.260804 7.1:-69470838 None:intergenic
AGATGCCTGGTTTGGAATTA+GGG 0.264298 7.1:+69470723 MS.gene88062:CDS
GATGGGCCTGATGGGCTTGA+TGG 0.267687 7.1:-69470463 None:intergenic
GCTTCAGCTTTAGCTTCTTC+TGG 0.304919 7.1:+69470365 MS.gene88062:CDS
CCTGTTGCAGCTATGATTGA+TGG 0.306906 7.1:-69470326 None:intergenic
GAACTGTGAGAATCATCTTT+AGG 0.314551 7.1:-69470860 None:intergenic
TTTCATCAATCATCATCTTC+TGG 0.335762 7.1:+69470554 MS.gene88062:CDS
TTTCAACTAACAAGAGAGTT+AGG 0.353942 7.1:+69470254 MS.gene88062:CDS
CTGGTTTACAGTTGAATGAT+AGG 0.368100 7.1:+69470423 MS.gene88062:CDS
ATGGGCCTGATGGGCCTGAT+GGG 0.381397 7.1:-69470471 None:intergenic
GATGGGCCTGATGGGCCTGA+TGG 0.383776 7.1:-69470472 None:intergenic
ATGGGCCTGATGGGCTTGAT+GGG 0.393154 7.1:-69470462 None:intergenic
ATGCCAACAGGAACAAATTT+GGG 0.405289 7.1:+69470653 MS.gene88062:CDS
TCAAGCCCATCAGGCCCATC+AGG 0.407369 7.1:+69470466 MS.gene88062:CDS
TCAAACCCATCAAGCCCATC+AGG 0.425492 7.1:+69470457 MS.gene88062:CDS
ACTATTGGTGAAAATAATAG+TGG 0.437127 7.1:+69470593 MS.gene88062:CDS
ATCATAGCTGCAACAGGAAC+AGG 0.443120 7.1:+69470332 MS.gene88062:CDS
GGTTTAGTAGCTACTACTAT+TGG 0.444798 7.1:+69470578 MS.gene88062:CDS
ACTCAGATTTATCAGCAGAT+TGG 0.447988 7.1:+69470791 MS.gene88062:CDS
TCTGGATTTGATATGCCAAC+AGG 0.449552 7.1:+69470641 MS.gene88062:CDS
ACTCCCAAATTTGTTCCTGT+TGG 0.452759 7.1:-69470656 None:intergenic
GTTGAATGATAGGAATAATT+GGG 0.466051 7.1:+69470433 MS.gene88062:CDS
AAATCAGCAGATGCCTGGTT+TGG 0.470684 7.1:+69470715 MS.gene88062:CDS
AACTAACATGATGACCTTGA+TGG 0.471715 7.1:-69470490 None:intergenic
ATGACCTTGATGGGCCTGAT+GGG 0.473600 7.1:-69470480 None:intergenic
CCATCAATCATAGCTGCAAC+AGG 0.479395 7.1:+69470326 MS.gene88062:CDS
TTGTCACAAGATGGACATAT+TGG 0.480930 7.1:+69470746 MS.gene88062:CDS
GAATTAGGGTTGTCACAAGA+TGG 0.488895 7.1:+69470737 MS.gene88062:CDS
TGGCCTCATCATCAAGTTGG+TGG 0.511204 7.1:+69470524 MS.gene88062:CDS
GGTGCAAATCAGCAGATGCC+TGG 0.519917 7.1:+69470710 MS.gene88062:CDS
CAGATGGTGAAACAATTCAA+TGG 0.529264 7.1:+69470285 MS.gene88062:CDS
AGATTGGTCAAAATCAAACT+AGG 0.530078 7.1:+69470807 MS.gene88062:CDS
ATTCTTGCTGCACATAAAGC+TGG 0.536084 7.1:-69470230 None:intergenic
AACAGGAACAAATTTGGGAG+TGG 0.545963 7.1:+69470658 MS.gene88062:CDS
CAGGCCCATCAGGCCCATCA+AGG 0.557193 7.1:+69470476 MS.gene88062:CDS
GATGACCTTGATGGGCCTGA+TGG 0.557322 7.1:-69470481 None:intergenic
ACTAACATGATGACCTTGAT+GGG 0.557914 7.1:-69470489 None:intergenic
CATCAATCATCATCTTCTGG+TGG 0.564429 7.1:+69470557 MS.gene88062:CDS
GGAACAAATTTGGGAGTGGG+AGG 0.568972 7.1:+69470662 MS.gene88062:CDS
GAAGAAGCTAAAGCTGAAGC+TGG 0.576280 7.1:-69470362 None:intergenic
GGTAATACTTTGACACCACA+AGG 0.588387 7.1:+69470386 MS.gene88062:CDS
ACAGGAACAAATTTGGGAGT+GGG 0.594534 7.1:+69470659 MS.gene88062:CDS
GATTGGTCAAAATCAAACTA+GGG 0.595101 7.1:+69470808 MS.gene88062:CDS
GAACAAATTTGGGAGTGGGA+GGG 0.598189 7.1:+69470663 MS.gene88062:CDS
TTATGGCCTCATCATCAAGT+TGG 0.605767 7.1:+69470521 MS.gene88062:CDS
GAGTTAGGTCATAAATCAGA+TGG 0.610512 7.1:+69470269 MS.gene88062:CDS
TATCCACCAACTTGATGATG+AGG 0.610804 7.1:-69470527 None:intergenic
GACAACCCTAATTCCAAACC+AGG 0.628289 7.1:-69470728 None:intergenic
GAAGACAAAGATGAACCTTG+TGG 0.735014 7.1:-69470401 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GAAATTATTTTCAGAAAATT+GGG + chr7.1:69470615-69470634 MS.gene88062:CDS 15.0%
!!! ATGAGTTTTACATCAATTTT+GGG + chr7.1:69470686-69470705 MS.gene88062:CDS 20.0%
!!! GGAAATTATTTTCAGAAAAT+TGG + chr7.1:69470614-69470633 MS.gene88062:CDS 20.0%
! ACTATTGGTGAAAATAATAG+TGG + chr7.1:69470593-69470612 MS.gene88062:CDS 25.0%
! AGTTGAATGATAGGAATAAT+TGG + chr7.1:69470432-69470451 MS.gene88062:CDS 25.0%
! GTTGAATGATAGGAATAATT+GGG + chr7.1:69470433-69470452 MS.gene88062:CDS 25.0%
!! GATGAGTTTTACATCAATTT+TGG + chr7.1:69470685-69470704 MS.gene88062:CDS 25.0%
!! TGAGTTTTACATCAATTTTG+GGG + chr7.1:69470687-69470706 MS.gene88062:CDS 25.0%
!!! ATGTTAGTTCTACAAGTTTA+TGG + chr7.1:69470504-69470523 MS.gene88062:CDS 25.0%
TTTCAACTAACAAGAGAGTT+AGG + chr7.1:69470254-69470273 MS.gene88062:CDS 30.0%
TTTCATCAATCATCATCTTC+TGG + chr7.1:69470554-69470573 MS.gene88062:CDS 30.0%
! AGATTGGTCAAAATCAAACT+AGG + chr7.1:69470807-69470826 MS.gene88062:CDS 30.0%
! AGTTTTACATCAATTTTGGG+GGG + chr7.1:69470689-69470708 MS.gene88062:CDS 30.0%
! GAGTTTTACATCAATTTTGG+GGG + chr7.1:69470688-69470707 MS.gene88062:CDS 30.0%
! GATTGGTCAAAATCAAACTA+GGG + chr7.1:69470808-69470827 MS.gene88062:CDS 30.0%
! TTTCAGAAAATTGGGTTTTC+TGG + chr7.1:69470623-69470642 MS.gene88062:CDS 30.0%
AACTAACATGATGACCTTGA+TGG - chr7.1:69470493-69470512 None:intergenic 35.0%
ACTAACATGATGACCTTGAT+GGG - chr7.1:69470492-69470511 None:intergenic 35.0%
ACTCAGATTTATCAGCAGAT+TGG + chr7.1:69470791-69470810 MS.gene88062:CDS 35.0%
ATGCCAACAGGAACAAATTT+GGG + chr7.1:69470653-69470672 MS.gene88062:CDS 35.0%
CAGATGGTGAAACAATTCAA+TGG + chr7.1:69470285-69470304 MS.gene88062:CDS 35.0%
CTGGTTTACAGTTGAATGAT+AGG + chr7.1:69470423-69470442 MS.gene88062:CDS 35.0%
GAACTGTGAGAATCATCTTT+AGG - chr7.1:69470863-69470882 None:intergenic 35.0%
GAGTTAGGTCATAAATCAGA+TGG + chr7.1:69470269-69470288 MS.gene88062:CDS 35.0%
GGTTTAGTAGCTACTACTAT+TGG + chr7.1:69470578-69470597 MS.gene88062:CDS 35.0%
TATGCCAACAGGAACAAATT+TGG + chr7.1:69470652-69470671 MS.gene88062:CDS 35.0%
TTGTCACAAGATGGACATAT+TGG + chr7.1:69470746-69470765 MS.gene88062:CDS 35.0%
!! GAGTATTATTCTGCTGATTC+TGG - chr7.1:69470841-69470860 None:intergenic 35.0%
AACAGGAACAAATTTGGGAG+TGG + chr7.1:69470658-69470677 MS.gene88062:CDS 40.0%
ACAGGAACAAATTTGGGAGT+GGG + chr7.1:69470659-69470678 MS.gene88062:CDS 40.0%
ATTCTTGCTGCACATAAAGC+TGG - chr7.1:69470233-69470252 None:intergenic 40.0%
CAAGGTTCATCTTTGTCTTC+TGG + chr7.1:69470404-69470423 MS.gene88062:CDS 40.0%
CATCAATCATCATCTTCTGG+TGG + chr7.1:69470557-69470576 MS.gene88062:CDS 40.0%
GAAGACAAAGATGAACCTTG+TGG - chr7.1:69470404-69470423 None:intergenic 40.0%
GAATTAGGGTTGTCACAAGA+TGG + chr7.1:69470737-69470756 MS.gene88062:CDS 40.0%
GGTAATACTTTGACACCACA+AGG + chr7.1:69470386-69470405 MS.gene88062:CDS 40.0%
TCTGGATTTGATATGCCAAC+AGG + chr7.1:69470641-69470660 MS.gene88062:CDS 40.0%
TTATGGCCTCATCATCAAGT+TGG + chr7.1:69470521-69470540 MS.gene88062:CDS 40.0%
! ACTCCCAAATTTGTTCCTGT+TGG - chr7.1:69470659-69470678 None:intergenic 40.0%
!! AGATGCCTGGTTTGGAATTA+GGG + chr7.1:69470723-69470742 MS.gene88062:CDS 40.0%
!! CATCATCAAGTTGGTGGATA+TGG + chr7.1:69470530-69470549 MS.gene88062:CDS 40.0%
!! TATCCACCAACTTGATGATG+AGG - chr7.1:69470530-69470549 None:intergenic 40.0%
AAATCAGCAGATGCCTGGTT+TGG + chr7.1:69470715-69470734 MS.gene88062:CDS 45.0%
ATCATAGCTGCAACAGGAAC+AGG + chr7.1:69470332-69470351 MS.gene88062:CDS 45.0%
CCATCAATCATAGCTGCAAC+AGG + chr7.1:69470326-69470345 MS.gene88062:CDS 45.0%
CCTGTTGCAGCTATGATTGA+TGG - chr7.1:69470329-69470348 None:intergenic 45.0%
GAACAAATTTGGGAGTGGGA+GGG + chr7.1:69470663-69470682 MS.gene88062:CDS 45.0%
GAAGAAGCTAAAGCTGAAGC+TGG - chr7.1:69470365-69470384 None:intergenic 45.0%
GACAACCCTAATTCCAAACC+AGG - chr7.1:69470731-69470750 None:intergenic 45.0%
! GCTTCAGCTTTAGCTTCTTC+TGG + chr7.1:69470365-69470384 MS.gene88062:CDS 45.0%
!! CAGATGCCTGGTTTGGAATT+AGG + chr7.1:69470722-69470741 MS.gene88062:CDS 45.0%
GGAACAAATTTGGGAGTGGG+AGG + chr7.1:69470662-69470681 MS.gene88062:CDS 50.0%
TCAAACCCATCAAGCCCATC+AGG + chr7.1:69470457-69470476 MS.gene88062:CDS 50.0%
! ATGACCTTGATGGGCCTGAT+GGG - chr7.1:69470483-69470502 None:intergenic 50.0%
!! TGGCCTCATCATCAAGTTGG+TGG + chr7.1:69470524-69470543 MS.gene88062:CDS 50.0%
GGTGCAAATCAGCAGATGCC+TGG + chr7.1:69470710-69470729 MS.gene88062:CDS 55.0%
! GATGACCTTGATGGGCCTGA+TGG - chr7.1:69470484-69470503 None:intergenic 55.0%
!! ATGGGCCTGATGGGCTTGAT+GGG - chr7.1:69470465-69470484 None:intergenic 55.0%
TCAAGCCCATCAGGCCCATC+AGG + chr7.1:69470466-69470485 MS.gene88062:CDS 60.0%
! ATGGGCCTGATGGGCCTGAT+GGG - chr7.1:69470474-69470493 None:intergenic 60.0%
!! GATGGGCCTGATGGGCTTGA+TGG - chr7.1:69470466-69470485 None:intergenic 60.0%
CAGGCCCATCAGGCCCATCA+AGG + chr7.1:69470476-69470495 MS.gene88062:CDS 65.0%
! GATGGGCCTGATGGGCCTGA+TGG - chr7.1:69470475-69470494 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr7.1 gene 69470227 69470892 69470227 ID=MS.gene88062
chr7.1 mRNA 69470227 69470892 69470227 ID=MS.gene88062.t1;Parent=MS.gene88062
chr7.1 exon 69470227 69470892 69470227 ID=MS.gene88062.t1.exon1;Parent=MS.gene88062.t1
chr7.1 CDS 69470227 69470892 69470227 ID=cds.MS.gene88062.t1;Parent=MS.gene88062.t1
Gene Sequence

>MS.gene88062

ATGCCAGCTTTATGTGCAGCAAGAATCTTTCAACTAACAAGAGAGTTAGGTCATAAATCAGATGGTGAAACAATTCAATGGCTTTTACAACAATCTGAACCATCAATCATAGCTGCAACAGGAACAGGAACAATACCAGCTTCAGCTTTAGCTTCTTCTGGTAATACTTTGACACCACAAGGTTCATCTTTGTCTTCTGGTTTACAGTTGAATGATAGGAATAATTGGGCTCAAACCCATCAAGCCCATCAGGCCCATCAGGCCCATCAAGGTCATCATGTTAGTTCTACAAGTTTATGGCCTCATCATCAAGTTGGTGGATATGGATTTCATCAATCATCATCTTCTGGTGGTTTAGTAGCTACTACTATTGGTGAAAATAATAGTGGAAATTATTTTCAGAAAATTGGGTTTTCTGGATTTGATATGCCAACAGGAACAAATTTGGGAGTGGGAGGGATGAGTTTTACATCAATTTTGGGGGGTGCAAATCAGCAGATGCCTGGTTTGGAATTAGGGTTGTCACAAGATGGACATATTGGTGTGTTGAATCAACAAGCTTTGACTCAGATTTATCAGCAGATTGGTCAAAATCAAACTAGGGTTCAGCACCAGAATCAGCAGAATAATACTCCTAAAGATGATTCTCACAGTTCAGAACAGTAG

Protein sequence

>MS.gene88062.t1

MPALCAARIFQLTRELGHKSDGETIQWLLQQSEPSIIAATGTGTIPASALASSGNTLTPQGSSLSSGLQLNDRNNWAQTHQAHQAHQAHQGHHVSSTSLWPHHQVGGYGFHQSSSSGGLVATTIGENNSGNYFQKIGFSGFDMPTGTNLGVGGMSFTSILGGANQQMPGLELGLSQDGHIGVLNQQALTQIYQQIGQNQTRVQHQNQQNNTPKDDSHSSEQ