AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene89434


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene89434.t1 MTR_3g043750 98.980 98 1 0 1 98 1 98 6.61e-68 198
MS.gene89434.t1 MTR_5g088770 63.000 100 34 1 1 97 1 100 7.14e-40 128
MS.gene89434.t1 MTR_8g041410 63.265 98 35 1 1 97 38 135 8.24e-39 129
MS.gene89434.t1 MTR_3g451210 48.936 94 48 0 4 97 31 124 1.97e-30 105
MS.gene89434.t1 MTR_3g452280 48.936 94 48 0 4 97 31 124 1.97e-30 105
MS.gene89434.t1 MTR_4g133540 42.308 78 45 0 15 92 38 115 4.73e-16 68.2
MS.gene89434.t1 MTR_3g451850 46.250 80 40 2 20 97 78 156 3.16e-14 66.6
MS.gene89434.t1 MTR_0526s0020 37.179 78 49 0 18 95 27 104 5.00e-13 62.8
MS.gene89434.t1 MTR_0526s0020 44.186 86 42 3 17 97 100 184 6.15e-12 59.7
MS.gene89434.t1 MTR_2g026040 36.264 91 57 1 2 92 27 116 7.63e-13 59.7
MS.gene89434.t1 MTR_8g098380 36.709 79 50 0 8 86 62 140 4.02e-11 57.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene89434.t1 AT3G12490 68.041 97 31 0 1 97 1 97 1.33e-44 142
MS.gene89434.t1 AT3G12490 68.041 97 31 0 1 97 34 130 1.68e-43 141
MS.gene89434.t1 AT2G40880 63.218 87 32 0 4 90 35 121 5.61e-38 124
MS.gene89434.t1 AT5G12140 56.044 91 40 0 4 94 10 100 3.63e-34 113
MS.gene89434.t1 AT5G05110 55.682 88 38 1 4 90 45 132 1.36e-27 100
MS.gene89434.t1 AT5G47550 34.444 90 57 2 2 90 26 114 4.89e-12 57.8
MS.gene89434.t1 AT4G16500 42.254 71 40 1 21 90 45 115 3.18e-11 55.8
MS.gene89434.t1 AT4G16500 42.254 71 40 1 21 90 45 115 3.18e-11 55.8

Find 18 sgRNAs with CRISPR-Local

Find 96 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTGGAGTTCGCGATGTTTC+AGG 0.215517 3.3:+31825646 MS.gene89434:CDS
TGTTATGCAGAATGCACTTC+TGG 0.324506 3.3:+31827075 MS.gene89434:intron
AAAAGATGGAGTGAATAAAA+AGG 0.356439 3.3:+31827168 MS.gene89434:CDS
CATCGATCGCAAGACTGTTC+TGG 0.359811 3.3:-31825672 None:intergenic
CATACACCACATCACTTTAG+AGG 0.393728 3.3:+31827144 MS.gene89434:CDS
GGTTTATGAAGCCAAGGTTT+GGG 0.416419 3.3:+31827189 MS.gene89434:CDS
AGGTTTATGAAGCCAAGGTT+TGG 0.435602 3.3:+31827188 MS.gene89434:CDS
GCTAAAGAGCAAGTGGTTGC+TGG 0.452937 3.3:+31827118 MS.gene89434:CDS
CCAAGGTTTGGGAAAAGTCT+TGG 0.461362 3.3:+31827200 MS.gene89434:CDS
CTCTAAAGTGATGTGGTGTA+TGG 0.517466 3.3:-31827143 None:intergenic
ATCACTTTAGAGGTAAAAGA+TGG 0.523463 3.3:+31827154 MS.gene89434:CDS
AAAGTCTTGGATGAACTTCA+AGG 0.534205 3.3:+31827213 MS.gene89434:CDS
AGTTAAGTGCTTTACTGCGC+AGG 0.534481 3.3:-31827268 None:intergenic
AGAAGAATGGCAACGCTTGG+TGG 0.543963 3.3:+31825628 None:intergenic
AGAAGAAGAATGGCAACGCT+TGG 0.566845 3.3:+31825625 None:intergenic
CAGAACAGTCTTGCGATCGA+TGG 0.591248 3.3:+31825673 MS.gene89434:CDS
GATAAGTGCTAAAGAGCAAG+TGG 0.632798 3.3:+31827111 MS.gene89434:CDS
GTCTTGGATGAACTTCAAGG+AGG 0.758137 3.3:+31827216 MS.gene89434:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAATAAGTTGTTAAATATA+TGG - chr3.3:31826372-31826391 None:intergenic 10.0%
!! GAACAAAATAAATAAATAAA+CGG - chr3.3:31826964-31826983 None:intergenic 10.0%
!!! ATTTTAAAGAATATGATATA+AGG - chr3.3:31826469-31826488 None:intergenic 10.0%
!!! TTTTATCATTTTTATTTAGT+TGG + chr3.3:31827048-31827067 MS.gene89434:intron 10.0%
!! ACTTTATAAATGTTTCAAAA+AGG - chr3.3:31826524-31826543 None:intergenic 15.0%
!! CTACATGATAAATAAAAATT+GGG - chr3.3:31825936-31825955 None:intergenic 15.0%
!! TAAAAAAAATTACAAGATGA+AGG - chr3.3:31825874-31825893 None:intergenic 15.0%
!!! ATTTTGAGAAATACTAAAAT+TGG + chr3.3:31826768-31826787 MS.gene89434:intron 15.0%
!!! TCTCAAATTTTTAATTAGAA+AGG + chr3.3:31826664-31826683 MS.gene89434:intron 15.0%
!!! TTTTTAGTAAATATTCGATT+TGG + chr3.3:31825888-31825907 MS.gene89434:intron 15.0%
!! GCTACATGATAAATAAAAAT+TGG - chr3.3:31825937-31825956 None:intergenic 20.0%
!! TTAACAACTTACTCTTAATT+AGG + chr3.3:31826410-31826429 MS.gene89434:intron 20.0%
!! TTGAAACATTTATAAAGTCA+AGG + chr3.3:31826526-31826545 MS.gene89434:intron 20.0%
!!! GCAATTTTCTTTAGTTTTTT+TGG + chr3.3:31827009-31827028 MS.gene89434:intron 20.0%
!!! TCGTAATTTTGTCAAAAAAA+GGG - chr3.3:31826068-31826087 None:intergenic 20.0%
! AAAAGATGGAGTGAATAAAA+AGG + chr3.3:31827168-31827187 MS.gene89434:CDS 25.0%
! AAAATAAGTAAAGACCCAAT+TGG - chr3.3:31826095-31826114 None:intergenic 25.0%
! ATAGAAAGTGGATAAACTTA+AGG - chr3.3:31826437-31826456 None:intergenic 25.0%
! TAATCAAGTTATGCTATGAA+AGG - chr3.3:31825758-31825777 None:intergenic 25.0%
! TATCCACTTTCTATCAATTT+AGG + chr3.3:31826443-31826462 MS.gene89434:intron 25.0%
! TTATGAGTAGTATGTCAAAA+CGG + chr3.3:31826317-31826336 MS.gene89434:intron 25.0%
!! CGTAATTTTGTCAAAAAAAG+GGG - chr3.3:31826067-31826086 None:intergenic 25.0%
!! CTCGTAATTTTGTCAAAAAA+AGG - chr3.3:31826069-31826088 None:intergenic 25.0%
AAAATTACGAGAGACCAATT+GGG + chr3.3:31826078-31826097 MS.gene89434:intron 30.0%
AAAGAGTGAAGTTACATTCA+AGG - chr3.3:31826899-31826918 None:intergenic 30.0%
ACTTGACACTTTAATAGAGT+CGG - chr3.3:31826345-31826364 None:intergenic 30.0%
CAATTGTTGAATCTATCGAT+TGG + chr3.3:31826618-31826637 MS.gene89434:intron 30.0%
CTTCTCTTATTCACTTTAAG+AGG - chr3.3:31825978-31825997 None:intergenic 30.0%
TAAAAAGGTTTATGAAGCCA+AGG + chr3.3:31827183-31827202 MS.gene89434:CDS 30.0%
TCTTTCAGTACTAAATGACT+TGG + chr3.3:31826914-31826933 MS.gene89434:intron 30.0%
TGAAGAACACAACAAAAAAC+AGG + chr3.3:31825714-31825733 MS.gene89434:CDS 30.0%
TGTCAAGTAACCTAATAAGT+GGG - chr3.3:31826259-31826278 None:intergenic 30.0%
TTGTCAAGTAACCTAATAAG+TGG - chr3.3:31826260-31826279 None:intergenic 30.0%
! ACTCTATTAAAGTGTCAAGT+TGG + chr3.3:31826345-31826364 MS.gene89434:intron 30.0%
! ATCACTTTAGAGGTAAAAGA+TGG + chr3.3:31827154-31827173 MS.gene89434:CDS 30.0%
! TGGTTTTCTCGGTAAAAATA+TGG + chr3.3:31826576-31826595 MS.gene89434:intron 30.0%
!!! AAATAAACGGCTTTAGCTTT+TGG - chr3.3:31826951-31826970 None:intergenic 30.0%
AAAGTCTTGGATGAACTTCA+AGG + chr3.3:31827213-31827232 MS.gene89434:CDS 35.0%
AATTTGAGAGATCTCACTGA+AGG - chr3.3:31826653-31826672 None:intergenic 35.0%
ACAGTGAATACTATATGCAG+GGG + chr3.3:31826211-31826230 MS.gene89434:intron 35.0%
ACATACTACTCATAACCTCA+AGG - chr3.3:31826312-31826331 None:intergenic 35.0%
AGGCCTAAATTGATAGAAAG+TGG - chr3.3:31826449-31826468 None:intergenic 35.0%
CAAAATTACGAGAGACCAAT+TGG + chr3.3:31826077-31826096 MS.gene89434:intron 35.0%
CAATATCGAGCTATACTCAA+GGG + chr3.3:31826028-31826047 MS.gene89434:intron 35.0%
CCAACACTACATTGTTTCAA+TGG - chr3.3:31826730-31826749 None:intergenic 35.0%
CCATTGAAACAATGTAGTGT+TGG + chr3.3:31826727-31826746 MS.gene89434:intron 35.0%
CTTGACACTTTAATAGAGTC+GGG - chr3.3:31826344-31826363 None:intergenic 35.0%
GACAGTGAATACTATATGCA+GGG + chr3.3:31826210-31826229 MS.gene89434:intron 35.0%
GTCAAGTAACCTAATAAGTG+GGG - chr3.3:31826258-31826277 None:intergenic 35.0%
TGTATAAAGTTTACGATGCG+AGG - chr3.3:31826836-31826855 None:intergenic 35.0%
TGTTCCTTAAACAAGATCAC+AGG - chr3.3:31826696-31826715 None:intergenic 35.0%
TTGAGTATAGCTCGATATTG+CGG - chr3.3:31826028-31826047 None:intergenic 35.0%
TTTGTGACAAAAGCGATCAT+CGG - chr3.3:31825828-31825847 None:intergenic 35.0%
!!! ATGCACTTCTGGAATTTTCA+AGG + chr3.3:31827086-31827105 MS.gene89434:CDS 35.0%
!!! CTTTAGCTTTTGGTAAGAGA+CGG - chr3.3:31826941-31826960 None:intergenic 35.0%
!!! CTTTTACCTCTAAAGTGATG+TGG - chr3.3:31827153-31827172 None:intergenic 35.0%
!!! TGCACTTCTGGAATTTTCAA+GGG + chr3.3:31827087-31827106 MS.gene89434:CDS 35.0%
ACTTAGACACCCCACTTATT+AGG + chr3.3:31826246-31826265 MS.gene89434:intron 40.0%
AGGTTTATGAAGCCAAGGTT+TGG + chr3.3:31827188-31827207 MS.gene89434:CDS 40.0%
ATAACCTCAAGGACCTACTT+CGG - chr3.3:31826301-31826320 None:intergenic 40.0%
ATACCAACTGAGCTAAACTC+AGG - chr3.3:31826181-31826200 None:intergenic 40.0%
CATACACCACATCACTTTAG+AGG + chr3.3:31827144-31827163 MS.gene89434:CDS 40.0%
CGACAGTGAATACTATATGC+AGG + chr3.3:31826209-31826228 MS.gene89434:intron 40.0%
GACTGCTAGATTCATACAAG+AGG + chr3.3:31826873-31826892 MS.gene89434:intron 40.0%
GATAAGTGCTAAAGAGCAAG+TGG + chr3.3:31827111-31827130 MS.gene89434:CDS 40.0%
GCAATATCGAGCTATACTCA+AGG + chr3.3:31826027-31826046 MS.gene89434:intron 40.0%
GGTTTATGAAGCCAAGGTTT+GGG + chr3.3:31827189-31827208 MS.gene89434:CDS 40.0%
TACCAACTGAGCTAAACTCA+GGG - chr3.3:31826180-31826199 None:intergenic 40.0%
TGTTATGCAGAATGCACTTC+TGG + chr3.3:31827075-31827094 MS.gene89434:intron 40.0%
! ATTGCAGGGCACTCTTAAAT+AGG - chr3.3:31826553-31826572 None:intergenic 40.0%
! CGAACCTGTGATCTTGTTTA+AGG + chr3.3:31826689-31826708 MS.gene89434:intron 40.0%
! GTTGAATCTATCGATTGGTC+TGG + chr3.3:31826623-31826642 MS.gene89434:intron 40.0%
! TGAGTTTAGCTCAGTTGGTA+TGG + chr3.3:31826180-31826199 MS.gene89434:intron 40.0%
!! ATTTAAGAGTGCCCTGCAAT+TGG + chr3.3:31826553-31826572 MS.gene89434:intron 40.0%
!! CTCTAAAGTGATGTGGTGTA+TGG - chr3.3:31827146-31827165 None:intergenic 40.0%
AACTGAGCTAAACTCAGGGA+CGG - chr3.3:31826176-31826195 None:intergenic 45.0%
AATACTATATGCAGGGGCTG+AGG + chr3.3:31826217-31826236 MS.gene89434:intron 45.0%
CCAAGGTTTGGGAAAAGTCT+TGG + chr3.3:31827200-31827219 MS.gene89434:CDS 45.0%
CGAGAAAACCACCAATTGCA+GGG - chr3.3:31826567-31826586 None:intergenic 45.0%
GTCTTGGATGAACTTCAAGG+AGG + chr3.3:31827216-31827235 MS.gene89434:CDS 45.0%
! CCAAGACTTTTCCCAAACCT+TGG - chr3.3:31827203-31827222 None:intergenic 45.0%
!! ATTGAGTTGACCTCCGAAGT+AGG + chr3.3:31826285-31826304 MS.gene89434:intron 45.0%
!! CCTGCAATTGGTGGTTTTCT+CGG + chr3.3:31826565-31826584 MS.gene89434:intron 45.0%
!! GAATCTATCGATTGGTCTGG+AGG + chr3.3:31826626-31826645 MS.gene89434:intron 45.0%
!!! TTTTATTTTTGATTTTTTTA+GGG + chr3.3:31826117-31826136 MS.gene89434:intron 5.0%
!!! TTTTTATTTTTGATTTTTTT+AGG + chr3.3:31826116-31826135 MS.gene89434:intron 5.0%
AGCTCGATATTGCGGTATGC+AGG - chr3.3:31826020-31826039 None:intergenic 50.0%
CAGAACAGTCTTGCGATCGA+TGG + chr3.3:31825673-31825692 MS.gene89434:CDS 50.0%
CATCGATCGCAAGACTGTTC+TGG - chr3.3:31825675-31825694 None:intergenic 50.0%
CCGAGAAAACCACCAATTGC+AGG - chr3.3:31826568-31826587 None:intergenic 50.0%
GCTAAAGAGCAAGTGGTTGC+TGG + chr3.3:31827118-31827137 MS.gene89434:CDS 50.0%
! GTCCCTGAGTTTAGCTCAGT+TGG + chr3.3:31826175-31826194 MS.gene89434:intron 50.0%
!! TAAGAGTGCCCTGCAATTGG+TGG + chr3.3:31826556-31826575 MS.gene89434:intron 50.0%
ACCTCAAGGACCTACTTCGG+AGG - chr3.3:31826298-31826317 None:intergenic 55.0%
ACCTCCGAAGTAGGTCCTTG+AGG + chr3.3:31826294-31826313 MS.gene89434:intron 55.0%
GGTGGAGTTCGCGATGTTTC+AGG + chr3.3:31825646-31825665 MS.gene89434:CDS 55.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 31825634 31827279 31825634 ID=MS.gene89434
chr3.3 mRNA 31825634 31827279 31825634 ID=MS.gene89434.t1;Parent=MS.gene89434
chr3.3 exon 31825634 31825735 31825634 ID=MS.gene89434.t1.exon1;Parent=MS.gene89434.t1
chr3.3 CDS 31825634 31825735 31825634 ID=cds.MS.gene89434.t1;Parent=MS.gene89434.t1
chr3.3 exon 31827085 31827279 31827085 ID=MS.gene89434.t1.exon2;Parent=MS.gene89434.t1
chr3.3 CDS 31827085 31827279 31827085 ID=cds.MS.gene89434.t1;Parent=MS.gene89434.t1
Gene Sequence

>MS.gene89434

ATGGCAACGCTTGGTGGAGTTCGCGATGTTTCAGGAAACCAGAACAGTCTTGCGATCGATGGTCTCGCTCGCTTTGCTGTTGAAGAACACAACAAAAAACAGGTTTCTTTTCTTTTTCTTTCCTTTCATAGCATAACTTGATTATTCTACGTTTCTTTGTTTGTTTTATGTTAATTTGTTATTGCGTCATGCCGATGATCGCTTTTGTCACAAATACGCATGATTTTTTTATTAGATCCTTCATCTTGTAATTTTTTTTAGTAAATATTCGATTTGGTTCTTCACAATTTTCTCAAAAACCCAATTTTTATTTATCATGTAGCTTAGTCGCTAGATATTCACCTCTTAAAGTGAATAAGAGAAGAATTCGTGTTAGAACGACGCCTGCATACCGCAATATCGAGCTATACTCAAGGGAACACTAAAAAAGCCCCTTTTTTTGACAAAATTACGAGAGACCAATTGGGTCTTTACTTATTTTTTTTTTATTTTTGATTTTTTTAGGGAGTAGTTTTTTCTCTTGTGAAAAATTGAGTTAACCGTCCCTGAGTTTAGCTCAGTTGGTATGGACAATGCGACAGTGAATACTATATGCAGGGGCTGAGGTTTGAAACTTAGACACCCCACTTATTAGGTTACTTGACAAAAAAAATTGAGTTGACCTCCGAAGTAGGTCCTTGAGGTTATGAGTAGTATGTCAAAACGGTCCCGACTCTATTAAAGTGTCAAGTTGGTCCATATATTTAACAACTTATTATCAATTAAGTCTCTATATTTTAACAACTTACTCTTAATTAGGTCCTTAAGTTTATCCACTTTCTATCAATTTAGGCCTTATATCATATTCTTTAAAATGATTATTACGAGTGAAATTTGAGAGTTAGAGACCTTTTTGAAACATTTATAAAGTCAAGGACCTATTTAAGAGTGCCCTGCAATTGGTGGTTTTCTCGGTAAAAATATGGTCGCTCTGCAAGTAATAAGCAATTGTTGAATCTATCGATTGGTCTGGAGGACCTTCAGTGAGATCTCTCAAATTTTTAATTAGAAAGGTTCGAACCTGTGATCTTGTTTAAGGAACATGAGTCTAGTTCCATTGAAACAATGTAGTGTTGGTTTGTCTAGTTGTATTTTATTTTGAGAAATACTAAAATTGGTTTTTGAAGTTCTTAAATATTCATTTTAGCTTTCAAATTAGTCCTCGCATCGTAAACTTTATACACAAAATACAACAAATTTGACTGCTAGATTCATACAAGAGGCCTTGAATGTAACTTCACTCTTTCAGTACTAAATGACTTGGACCGTCTCTTACCAAAAGCTAAAGCCGTTTATTTATTTATTTTGTTCATTTTGTAGTGTATAGATTTCAATTGCAATTTTCTTTAGTTTTTTTGGTGTGTGTTTTTTTTTTTTTTATCATTTTTATTTAGTTGGTGATTGTTATGCAGAATGCACTTCTGGAATTTTCAAGGGTGATAAGTGCTAAAGAGCAAGTGGTTGCTGGTACCATACACCACATCACTTTAGAGGTAAAAGATGGAGTGAATAAAAAGGTTTATGAAGCCAAGGTTTGGGAAAAGTCTTGGATGAACTTCAAGGAGGTGCAAGAGTTCAAGCTCGTCGAAGATGCACCTGCGCAGTAA

Protein sequence

>MS.gene89434.t1

MATLGGVRDVSGNQNSLAIDGLARFAVEEHNKKQNALLEFSRVISAKEQVVAGTIHHITLEVKDGVNKKVYEAKVWEKSWMNFKEVQEFKLVEDAPAQ