AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene89812


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene89812.t1 MTR_7g063670 85.652 230 32 1 1 229 229 458 5.61e-136 390
MS.gene89812.t1 MTR_3g007740 60.684 234 72 3 2 223 218 443 3.51e-84 257
MS.gene89812.t1 MTR_7g063920 50.607 247 97 6 2 229 197 437 5.97e-61 197
MS.gene89812.t1 MTR_7g063920 27.941 204 113 6 24 216 11 191 7.14e-13 67.4
MS.gene89812.t1 MTR_7g062280 44.882 254 107 5 2 229 246 492 7.83e-61 198
MS.gene89812.t1 MTR_7g062300 46.721 244 99 6 2 221 236 472 1.73e-56 186
MS.gene89812.t1 MTR_7g063720 44.939 247 104 7 1 229 1 233 3.15e-55 177
MS.gene89812.t1 MTR_7g063840 45.455 253 105 6 2 229 230 474 2.36e-53 178
MS.gene89812.t1 MTR_7g063660 43.701 254 114 5 1 229 229 478 8.70e-53 177
MS.gene89812.t1 MTR_7g063880 42.460 252 101 8 2 228 236 468 7.24e-46 159
MS.gene89812.t1 MTR_7g063820 39.216 255 125 5 2 229 234 485 4.74e-44 154
MS.gene89812.t1 MTR_7g063900 43.130 262 111 8 2 229 232 489 1.01e-42 150
MS.gene89812.t1 MTR_7g063830 43.220 236 102 4 2 228 232 444 1.50e-42 149
MS.gene89812.t1 MTR_7g063830 26.126 222 128 6 25 216 11 226 5.48e-11 62.0
MS.gene89812.t1 MTR_7g063850 40.164 244 111 8 13 228 52 288 3.92e-41 142
MS.gene89812.t1 MTR_7g066570 37.339 233 119 4 20 226 253 484 6.80e-40 144
MS.gene89812.t1 MTR_7g063760 86.486 74 10 0 156 229 1 74 1.03e-39 131
MS.gene89812.t1 MTR_7g066410 39.914 233 106 6 25 225 285 515 6.60e-38 138
MS.gene89812.t1 MTR_7g012680 39.070 215 125 4 20 229 239 452 1.17e-37 136
MS.gene89812.t1 MTR_7g066260 40.887 203 107 3 24 222 269 462 5.72e-37 135
MS.gene89812.t1 MTR_7g058710 38.197 233 116 4 24 229 253 484 2.96e-35 130
MS.gene89812.t1 MTR_7g066480 41.206 199 77 4 25 223 264 422 7.33e-35 129
MS.gene89812.t1 MTR_7g063890 42.146 261 113 9 2 228 248 504 9.67e-34 126
MS.gene89812.t1 MTR_7g058700 40.976 205 92 3 25 229 753 928 1.19e-33 128
MS.gene89812.t1 MTR_7g066400 37.073 205 128 1 14 218 230 433 3.34e-33 124
MS.gene89812.t1 MTR_7g066430 39.076 238 105 8 20 222 253 485 6.81e-32 121
MS.gene89812.t1 MTR_7g066490 38.579 197 81 4 27 223 252 408 3.34e-30 115
MS.gene89812.t1 MTR_7g066290 39.698 199 88 4 24 222 267 433 3.85e-30 116
MS.gene89812.t1 MTR_7g066280 36.929 241 108 5 25 222 151 390 7.22e-30 115
MS.gene89812.t1 MTR_7g066580 33.333 234 122 5 25 225 216 448 1.71e-29 114
MS.gene89812.t1 MTR_7g066360 38.265 196 105 4 24 219 267 446 3.06e-29 114
MS.gene89812.t1 MTR_7g066680 34.127 252 106 7 24 218 86 334 3.27e-29 112
MS.gene89812.t1 MTR_7g066310 36.869 198 117 3 25 222 194 383 1.35e-28 111
MS.gene89812.t1 MTR_7g100420 35.950 242 125 6 2 218 157 393 1.81e-28 111
MS.gene89812.t1 MTR_1g041315 38.624 189 105 5 20 206 272 451 9.93e-28 110
MS.gene89812.t1 MTR_7g066350 35.545 211 125 3 20 229 257 457 7.58e-27 107
MS.gene89812.t1 MTR_7g066340 35.354 198 120 2 25 222 264 453 9.11e-27 107
MS.gene89812.t1 MTR_7g063750 60.000 95 34 2 134 227 1 92 5.98e-25 98.2
MS.gene89812.t1 MTR_1g014060 35.519 183 110 2 54 229 210 391 1.21e-23 97.8
MS.gene89812.t1 MTR_4g019310 31.727 249 136 9 11 229 253 497 1.67e-23 98.2
MS.gene89812.t1 MTR_3g065200 30.275 218 111 6 18 209 206 408 7.51e-22 93.6
MS.gene89812.t1 MTR_7g066390 36.306 157 64 3 66 222 2 122 1.32e-17 77.0
MS.gene89812.t1 MTR_7g063690 64.706 51 18 0 1 51 172 222 1.23e-13 68.2
MS.gene89812.t1 MTR_2g029400 30.729 192 103 5 49 212 216 405 1.45e-12 66.6
MS.gene89812.t1 MTR_4g017610 28.293 205 133 5 27 218 272 475 6.76e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene89812.t1 AT5G01130 31.944 216 129 7 20 229 248 451 1.85e-18 84.0
MS.gene89812.t1 AT5G01140 28.063 253 131 8 9 229 225 458 2.78e-15 74.7
MS.gene89812.t1 AT5G37320 29.577 213 135 8 25 224 271 481 6.56e-13 67.8

Find 35 sgRNAs with CRISPR-Local

Find 48 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTGTAACCACGTACATTTC+TGG 0.179686 7.3:-41580556 None:intergenic
ATTAATATCATCATTAAATA+TGG 0.226870 7.3:-41580146 None:intergenic
CTTAGAAGGGAATAGTTCTT+TGG 0.229478 7.3:+41580291 MS.gene89812:CDS
CAAAACTTCTCTTAATGATC+TGG 0.348610 7.3:+41580639 MS.gene89812:CDS
AGTTCTTTGGGAAGTATTGA+TGG 0.349836 7.3:+41580304 MS.gene89812:CDS
AGTATATATATAGATGAAAC+TGG 0.371045 7.3:-41580607 None:intergenic
ATCCTTGATTTCTCGATATG+AGG 0.374371 7.3:-41580103 None:intergenic
GTGATGAAGATGGGAGGGAT+TGG 0.398301 7.3:+41580458 MS.gene89812:CDS
TTAGAAGGGAATAGTTCTTT+GGG 0.400686 7.3:+41580292 MS.gene89812:CDS
CGTTTCTTAACATTTCCATT+GGG 0.468800 7.3:+41580253 MS.gene89812:CDS
GCAGAGTCACAGCATATTCA+AGG 0.477264 7.3:+41580962 MS.gene89812:intron
ACGTTTCTTAACATTTCCAT+TGG 0.480881 7.3:+41580252 MS.gene89812:CDS
CTTGTTAACACAAATTAAGA+AGG 0.481190 7.3:+41581028 MS.gene89812:CDS
TTCTTAACATTTCCATTGGG+TGG 0.500846 7.3:+41580256 MS.gene89812:CDS
GACATTATATTATATGCTCA+AGG 0.503201 7.3:+41580202 MS.gene89812:CDS
TATCGAAGGATGCAAAGAAC+AGG 0.512053 7.3:+41580371 MS.gene89812:CDS
TCAACGTCTGCTCTAACAAA+TGG 0.520066 7.3:+41580996 MS.gene89812:CDS
GACTTGCCGCATGGTGGACC+TGG 0.523472 7.3:+41580517 MS.gene89812:CDS
AATTACCTCTTTGATGCCAA+TGG 0.525143 7.3:-41580677 None:intergenic
AACCTCATATCGAGAAATCA+AGG 0.529213 7.3:+41580101 MS.gene89812:CDS
TCCACCATGCGGCAAGTCAA+TGG 0.533548 7.3:-41580512 None:intergenic
TAATTTGTGTTAACAAGTGA+CGG 0.537543 7.3:-41581022 None:intergenic
GGTTTCTTGACATAAACTCC+AGG 0.555287 7.3:-41580535 None:intergenic
CATTATATTATATGCTCAAG+GGG 0.573209 7.3:+41580204 MS.gene89812:CDS
ACATTATATTATATGCTCAA+GGG 0.581121 7.3:+41580203 MS.gene89812:CDS
AGAAAAGTATTTCATATCGA+AGG 0.586970 7.3:+41580357 MS.gene89812:CDS
AACTTCTCTTAATGATCTGG+AGG 0.587581 7.3:+41580642 MS.gene89812:CDS
TTGCGCAAAACTCCACCCAA+TGG 0.594851 7.3:-41580268 None:intergenic
AATACCATTGACTTGCCGCA+TGG 0.596786 7.3:+41580508 MS.gene89812:CDS
ACCATTGACTTGCCGCATGG+TGG 0.629935 7.3:+41580511 MS.gene89812:CDS
TGTCACCATTGGCATCAAAG+AGG 0.672938 7.3:+41580672 MS.gene89812:CDS
CAAGAAACCAGAAATGTACG+TGG 0.689087 7.3:+41580549 MS.gene89812:CDS
GATGAAACTGGAGACAACAA+CGG 0.697583 7.3:-41580595 None:intergenic
TAAACTCCAGGTCCACCATG+CGG 0.736990 7.3:-41580523 None:intergenic
ATTATATTATATGCTCAAGG+GGG 0.741500 7.3:+41580205 MS.gene89812:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAATATCATCATTAAATA+TGG - chr7.3:41580149-41580168 None:intergenic 10.0%
!! ATCAAAGTGATAATTATTTA+AGG + chr7.3:41580715-41580734 MS.gene89812:intron 15.0%
!! ACATTATATTATATGCTCAA+GGG + chr7.3:41580203-41580222 MS.gene89812:CDS 20.0%
!! AGTATATATATAGATGAAAC+TGG - chr7.3:41580610-41580629 None:intergenic 20.0%
!!! AGTGTTTTGAAATATTATTG+TGG + chr7.3:41580406-41580425 MS.gene89812:CDS 20.0%
!!! TTTTAAAAAGTGATGAAGAT+GGG + chr7.3:41580449-41580468 MS.gene89812:CDS 20.0%
! ATTATATTATATGCTCAAGG+GGG + chr7.3:41580205-41580224 MS.gene89812:CDS 25.0%
! CATTATATTATATGCTCAAG+GGG + chr7.3:41580204-41580223 MS.gene89812:CDS 25.0%
! CTTGTTAACACAAATTAAGA+AGG + chr7.3:41581028-41581047 MS.gene89812:CDS 25.0%
! GACATTATATTATATGCTCA+AGG + chr7.3:41580202-41580221 MS.gene89812:CDS 25.0%
!! TAATTTGTGTTAACAAGTGA+CGG - chr7.3:41581025-41581044 None:intergenic 25.0%
!!! AGAAAAGTATTTCATATCGA+AGG + chr7.3:41580357-41580376 MS.gene89812:CDS 25.0%
!!! CTTTTAAAAAGTGATGAAGA+TGG + chr7.3:41580448-41580467 MS.gene89812:CDS 25.0%
ACATCTTAGAAAAATGCAAC+TGG + chr7.3:41580483-41580502 MS.gene89812:CDS 30.0%
ACGTTTCTTAACATTTCCAT+TGG + chr7.3:41580252-41580271 MS.gene89812:CDS 30.0%
CAAAACTTCTCTTAATGATC+TGG + chr7.3:41580639-41580658 MS.gene89812:CDS 30.0%
CGTTTCTTAACATTTCCATT+GGG + chr7.3:41580253-41580272 MS.gene89812:CDS 30.0%
TTAGAAGGGAATAGTTCTTT+GGG + chr7.3:41580292-41580311 MS.gene89812:CDS 30.0%
! TTATCACCTTCACTATTTTG+CGG + chr7.3:41580756-41580775 MS.gene89812:intron 30.0%
!! TTTTCTGATAACTAGCTTCA+CGG - chr7.3:41580176-41580195 None:intergenic 30.0%
AAAAAGTGATGAAGATGGGA+GGG + chr7.3:41580453-41580472 MS.gene89812:CDS 35.0%
AACCTCATATCGAGAAATCA+AGG + chr7.3:41580101-41580120 MS.gene89812:CDS 35.0%
AACTTCTCTTAATGATCTGG+AGG + chr7.3:41580642-41580661 MS.gene89812:CDS 35.0%
AATTACCTCTTTGATGCCAA+TGG - chr7.3:41580680-41580699 None:intergenic 35.0%
ATCCTTGATTTCTCGATATG+AGG - chr7.3:41580106-41580125 None:intergenic 35.0%
CTTAGAAGGGAATAGTTCTT+TGG + chr7.3:41580291-41580310 MS.gene89812:CDS 35.0%
TAAAAAGTGATGAAGATGGG+AGG + chr7.3:41580452-41580471 MS.gene89812:CDS 35.0%
TTCTTAACATTTCCATTGGG+TGG + chr7.3:41580256-41580275 MS.gene89812:CDS 35.0%
!! AGTTCTTTGGGAAGTATTGA+TGG + chr7.3:41580304-41580323 MS.gene89812:CDS 35.0%
ACACAACCGCAAAATAGTGA+AGG - chr7.3:41580765-41580784 None:intergenic 40.0%
CAAGAAACCAGAAATGTACG+TGG + chr7.3:41580549-41580568 MS.gene89812:CDS 40.0%
GAGGAAAAAGTTGTCACCAT+TGG + chr7.3:41580661-41580680 MS.gene89812:CDS 40.0%
GATGAAACTGGAGACAACAA+CGG - chr7.3:41580598-41580617 None:intergenic 40.0%
GGTTTCTTGACATAAACTCC+AGG - chr7.3:41580538-41580557 None:intergenic 40.0%
TATCGAAGGATGCAAAGAAC+AGG + chr7.3:41580371-41580390 MS.gene89812:CDS 40.0%
TCAACGTCTGCTCTAACAAA+TGG + chr7.3:41580996-41581015 MS.gene89812:CDS 40.0%
TCTGTAACCACGTACATTTC+TGG - chr7.3:41580559-41580578 None:intergenic 40.0%
! GAGTTTTGCGCAACTTAGAA+GGG + chr7.3:41580278-41580297 MS.gene89812:CDS 40.0%
GCAGAGTCACAGCATATTCA+AGG + chr7.3:41580962-41580981 MS.gene89812:intron 45.0%
TGTCACCATTGGCATCAAAG+AGG + chr7.3:41580672-41580691 MS.gene89812:CDS 45.0%
! GGAGTTTTGCGCAACTTAGA+AGG + chr7.3:41580277-41580296 MS.gene89812:CDS 45.0%
!! AATACCATTGACTTGCCGCA+TGG + chr7.3:41580508-41580527 MS.gene89812:CDS 45.0%
GTGATGAAGATGGGAGGGAT+TGG + chr7.3:41580458-41580477 MS.gene89812:CDS 50.0%
TAAACTCCAGGTCCACCATG+CGG - chr7.3:41580526-41580545 None:intergenic 50.0%
TTGCGCAAAACTCCACCCAA+TGG - chr7.3:41580271-41580290 None:intergenic 50.0%
TCCACCATGCGGCAAGTCAA+TGG - chr7.3:41580515-41580534 None:intergenic 55.0%
!! ACCATTGACTTGCCGCATGG+TGG + chr7.3:41580511-41580530 MS.gene89812:CDS 55.0%
!! GACTTGCCGCATGGTGGACC+TGG + chr7.3:41580517-41580536 MS.gene89812:CDS 65.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 41580097 41581058 41580097 ID=MS.gene89812
chr7.3 mRNA 41580097 41581058 41580097 ID=MS.gene89812.t1;Parent=MS.gene89812
chr7.3 exon 41580097 41580693 41580097 ID=MS.gene89812.t1.exon1;Parent=MS.gene89812.t1
chr7.3 CDS 41580097 41580693 41580097 ID=cds.MS.gene89812.t1;Parent=MS.gene89812.t1
chr7.3 exon 41580966 41581058 41580966 ID=MS.gene89812.t1.exon2;Parent=MS.gene89812.t1
chr7.3 CDS 41580966 41581058 41580966 ID=cds.MS.gene89812.t1;Parent=MS.gene89812.t1
Gene Sequence

>MS.gene89812

ATGAAACCTCATATCGAGAAATCAAGGATTTTCTCTGATGATGTTAAAACCATATTTAATGATGATATTAATATCACCGTGAAGCTAGTTATCAGAAAATCTGATGACATTATATTATATGCTCAAGGGGGACATGATTTTGCAGAGTTAATTATACGTTTCTTAACATTTCCATTGGGTGGAGTTTTGCGCAACTTAGAAGGGAATAGTTCTTTGGGAAGTATTGATGGATTGTGCAAGAGCATAGCTGATTTAAATGAAGAAAAGTATTTCATATCGAAGGATGCAAAGAACAGGCTTCTCAAGACAAGTGTTTTGAAATATTATTGTGGAAAGTTAACTGATCTCACACTTTTAAAAAGTGATGAAGATGGGAGGGATTGGAGACATCTTAGAAAAATGCAACTGGTTAATACCATTGACTTGCCGCATGGTGGACCTGGAGTTTATGTCAAGAAACCAGAAATGTACGTGGTTACAGATGATCTGATCGTAGAACCGTTGTTGTCTCCAGTTTCATCTATATATATACTTCAACGTTTCAAAACTTCTCTTAATGATCTGGAGGAAAAAGTTGTCACCATTGGCATCAAAGAGGTAATTATTTTTTGACAAGAAATCAAAGTGATAATTATTTAAGGAGTGAAAACAATATAGATTTATCACCTTCACTATTTTGCGGTTGTGTTGAAGTTTTCATACTAGACTGATTTACTATGAAACTTATTGAAGATATAGTTAGTAGTCATTTACTTGTTAGAAATTAGTTGTTTGCTATTCGTTTCTAACTAATGAATGACTACTAACTATATCATCAACAAGATTAATTATGTAGCAATAGCTCATCTCACATTGTTGTGTTTGTGCAGAGTCACAGCATATTCAAGGCAGCTTTAAGCTCAACGTCTGCTCTAACAAATGGTCTCCGTCACTTGTTAACACAAATTAAGAAGGAGAAATGA

Protein sequence

>MS.gene89812.t1

MKPHIEKSRIFSDDVKTIFNDDINITVKLVIRKSDDIILYAQGGHDFAELIIRFLTFPLGGVLRNLEGNSSLGSIDGLCKSIADLNEEKYFISKDAKNRLLKTSVLKYYCGKLTDLTLLKSDEDGRDWRHLRKMQLVNTIDLPHGGPGVYVKKPEMYVVTDDLIVEPLLSPVSSIYILQRFKTSLNDLEEKVVTIGIKESHSIFKAALSSTSALTNGLRHLLTQIKKEK