AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene92818


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene92818.t1 MTR_7g010150 100.000 120 0 0 1 120 1 120 4.69e-83 238
MS.gene92818.t1 MTR_8g467460 99.160 119 1 0 1 119 1 119 2.73e-81 234
MS.gene92818.t1 MTR_5g040570 96.667 120 4 0 1 120 1 120 2.85e-80 231
MS.gene92818.t1 MTR_7g011050 63.717 113 25 1 1 113 1 97 5.56e-44 139
MS.gene92818.t1 MTR_8g019410 47.863 117 23 2 4 119 47 126 1.49e-20 80.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene92818.t1 AT1G26880 90.833 120 11 0 1 120 1 120 2.57e-75 219
MS.gene92818.t1 AT1G69620 91.597 119 10 0 1 119 1 119 9.90e-75 217
MS.gene92818.t1 AT3G28900 88.333 120 14 0 1 120 1 120 4.98e-73 213
MS.gene92818.t1 AT1G26880 91.304 92 8 0 1 92 1 92 6.96e-58 174

Find 31 sgRNAs with CRISPR-Local

Find 85 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAACCGCCGCACTGTTAAC+AGG 0.305052 7.1:+85301195 MS.gene92818:CDS
TGGATCCTCTTTCCAGTAAC+AGG 0.314412 7.1:-85299935 None:intergenic
GGACCTAAGTGTCCTGTTAC+TGG 0.331250 7.1:+85299923 MS.gene92818:CDS
CGCACTGTTAACAGGGCTTA+TGG 0.356563 7.1:+85301203 MS.gene92818:CDS
AACACGATGCTGGTTGGATT+TGG 0.389836 7.1:-85299701 None:intergenic
TTAACAGTGCGGCGGTTCCT+AGG 0.468395 7.1:-85301191 None:intergenic
CCTTTCCCTAACAGCTCCTC+CGG 0.470814 7.1:-85301237 None:intergenic
TTTATCCGGAGGAGCTGTTA+GGG 0.501294 7.1:+85301232 MS.gene92818:CDS
TTTCCAGTAACAGGACACTT+AGG 0.509665 7.1:-85299926 None:intergenic
ACGATGCTGGTTGGATTTGG+TGG 0.532546 7.1:-85299698 None:intergenic
CATCGTGTTGTTAAGACACC+TGG 0.536949 7.1:+85299715 MS.gene92818:CDS
AGTGTCCTGTTACTGGAAAG+AGG 0.543803 7.1:+85299930 MS.gene92818:CDS
CTTAACAACACGATGCTGGT+TGG 0.545517 7.1:-85299707 None:intergenic
GTTACTGGAAAGAGGATCCA+AGG 0.571456 7.1:+85299938 MS.gene92818:CDS
ACAACTAAGAAGAGAGCTAG+TGG 0.577057 7.1:+85299902 MS.gene92818:CDS
GTGTCTTAACAACACGATGC+TGG 0.578379 7.1:-85299711 None:intergenic
TGGCATAGCTGTGTCTCCTA+CGG 0.580308 7.1:-85299678 None:intergenic
GCTCTTCAACAAGGAAAGCA+CGG 0.587996 7.1:-85301351 None:intergenic
CCGGAGGAGCTGTTAGGGAA+AGG 0.595594 7.1:+85301237 MS.gene92818:CDS
GAACCGCCGCACTGTTAACA+GGG 0.611783 7.1:+85301196 MS.gene92818:CDS
CTAGACCTCTTGTATTCAGT+AGG 0.617053 7.1:-85301164 None:intergenic
TTACTGGAAAGAGGATCCAA+GGG 0.629653 7.1:+85299939 MS.gene92818:CDS
TGAGACCTACTGAATACAAG+AGG 0.631121 7.1:+85301159 MS.gene92818:CDS
ACAAGAGGTCTAGATTGCCT+AGG 0.636078 7.1:+85301174 MS.gene92818:CDS
TATTCAGTAGGTCTCAAGTG+TGG 0.667813 7.1:-85301152 None:intergenic
AAGCCCTGTTAACAGTGCGG+CGG 0.691394 7.1:-85301199 None:intergenic
ACTGTTAACAGGGCTTATGG+AGG 0.693206 7.1:+85301206 MS.gene92818:CDS
TTCAGAGACTCACATACCGT+AGG 0.721913 7.1:+85299662 MS.gene92818:CDS
CATAAGCCCTGTTAACAGTG+CGG 0.730145 7.1:-85301202 None:intergenic
TACTGGAAAGAGGATCCAAG+GGG 0.738216 7.1:+85299940 MS.gene92818:CDS
GTATGTGAGTCTCTGAACCA+TGG 0.739024 7.1:-85299656 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTATAAAATCAATTATTTG+TGG + chr7.1:85300226-85300245 MS.gene92818:intron 10.0%
!! ATTTGAAAATTGAAAATATT+AGG - chr7.1:85300310-85300329 None:intergenic 10.0%
!!! TTTTTTAGAAATTAATATGT+TGG + chr7.1:85299984-85300003 MS.gene92818:intron 10.0%
!! TAACAAATCTTCAATAAAAA+TGG - chr7.1:85300638-85300657 None:intergenic 15.0%
!! TTTGCAATTATTAATGTTAA+TGG + chr7.1:85300191-85300210 MS.gene92818:intron 15.0%
!!! TTTGATTAATTTGTGAAAAT+GGG + chr7.1:85299844-85299863 MS.gene92818:intron 15.0%
!!! TTTTGATTAATTTGTGAAAA+TGG + chr7.1:85299843-85299862 MS.gene92818:intron 15.0%
!! GAATGAAAATTAAAAGTAGT+AGG - chr7.1:85301011-85301030 None:intergenic 20.0%
!!! TTAATACTACAATTTTGTGA+GGG - chr7.1:85300797-85300816 None:intergenic 20.0%
!!! TTAATATGTTGGTTATACAT+AGG + chr7.1:85299995-85300014 MS.gene92818:intron 20.0%
!!! TTTAATACTACAATTTTGTG+AGG - chr7.1:85300798-85300817 None:intergenic 20.0%
! AAACTATCTCTTCTGTAATA+GGG - chr7.1:85300497-85300516 None:intergenic 25.0%
! AATGTTCTTACTTCAATGTT+GGG + chr7.1:85300260-85300279 MS.gene92818:intron 25.0%
! AGATACCCTTTCTAAATTAT+TGG + chr7.1:85300817-85300836 MS.gene92818:intron 25.0%
! AGTCACCAATAATTTAGAAA+GGG - chr7.1:85300825-85300844 None:intergenic 25.0%
! ATCTATCATGTTTCAAACAT+AGG - chr7.1:85300662-85300681 None:intergenic 25.0%
! ATTGAATTCATCTATGTTAG+TGG - chr7.1:85300341-85300360 None:intergenic 25.0%
! GAAAATTAAAAGTAGTAGGT+AGG - chr7.1:85301007-85301026 None:intergenic 25.0%
! GACTTGTATTATTCATCAAT+TGG + chr7.1:85300111-85300130 MS.gene92818:intron 25.0%
! GATATATGTGAGTTTATGAT+AGG + chr7.1:85300089-85300108 MS.gene92818:intron 25.0%
! GCATCATTGAAATTATACAA+TGG - chr7.1:85300910-85300929 None:intergenic 25.0%
! TGTAAAATAGGTGGAATATT+AGG + chr7.1:85300156-85300175 MS.gene92818:intron 25.0%
! TTATCATATTTGTTCTGTTC+TGG + chr7.1:85301113-85301132 MS.gene92818:intron 25.0%
! TTGAATTCATCTATGTTAGT+GGG - chr7.1:85300340-85300359 None:intergenic 25.0%
! TTGTCTGTGTTATGTAAAAT+AGG + chr7.1:85300144-85300163 MS.gene92818:intron 25.0%
AAGTGTCAAGATTATATGCA+GGG - chr7.1:85300983-85301002 None:intergenic 30.0%
CAAACTATCTCTTCTGTAAT+AGG - chr7.1:85300498-85300517 None:intergenic 30.0%
CAACACAATATTCAATCAGA+TGG - chr7.1:85300467-85300486 None:intergenic 30.0%
CAGTCACCAATAATTTAGAA+AGG - chr7.1:85300826-85300845 None:intergenic 30.0%
GAATGTTCTTACTTCAATGT+TGG + chr7.1:85300259-85300278 MS.gene92818:intron 30.0%
TCTGTGTTATGTAAAATAGG+TGG + chr7.1:85300147-85300166 MS.gene92818:intron 30.0%
! AAAATTGATGATAGCGTCAA+AGG - chr7.1:85300708-85300727 None:intergenic 30.0%
! AAAGTGTCAAGATTATATGC+AGG - chr7.1:85300984-85301003 None:intergenic 30.0%
!!! AAATGGGTTTGTGTTGTTTT+AGG + chr7.1:85299860-85299879 MS.gene92818:intron 30.0%
!!! CTATTTTTTGCTCTTCAACA+AGG - chr7.1:85301363-85301382 None:intergenic 30.0%
!!! CTTGCTTTTCTTTTGTTTTC+TGG - chr7.1:85301405-85301424 None:intergenic 30.0%
CAATTCTACAACATTGTGGA+AGG + chr7.1:85300929-85300948 MS.gene92818:intron 35.0%
GATGCAATTCTACAACATTG+TGG + chr7.1:85300925-85300944 MS.gene92818:intron 35.0%
TCATATAGATTCATACCCCT+TGG - chr7.1:85299958-85299977 None:intergenic 35.0%
TGACAAAGAAGAACATTACC+AGG - chr7.1:85299736-85299755 None:intergenic 35.0%
TTTGTTTGTACATGATGCTG+TGG + chr7.1:85300053-85300072 MS.gene92818:intron 35.0%
! CAATTTTGTGAGGGACAATA+AGG - chr7.1:85300788-85300807 None:intergenic 35.0%
!!! TGTTGTTTTAGGTGGAAAGT+TGG + chr7.1:85299871-85299890 MS.gene92818:intron 35.0%
AAACTTAGACGCTGTGTGAT+AGG - chr7.1:85300884-85300903 None:intergenic 40.0%
ACAACTAAGAAGAGAGCTAG+TGG + chr7.1:85299902-85299921 MS.gene92818:CDS 40.0%
ATAGCGTCAAAGGTCATAGT+TGG - chr7.1:85300698-85300717 None:intergenic 40.0%
CTAGACCTCTTGTATTCAGT+AGG - chr7.1:85301167-85301186 None:intergenic 40.0%
CTTCACAAACACAGCTGAAT+TGG - chr7.1:85300580-85300599 None:intergenic 40.0%
TATTCAGTAGGTCTCAAGTG+TGG - chr7.1:85301155-85301174 None:intergenic 40.0%
TGAGACCTACTGAATACAAG+AGG + chr7.1:85301159-85301178 MS.gene92818:CDS 40.0%
TTACTGGAAAGAGGATCCAA+GGG + chr7.1:85299939-85299958 MS.gene92818:CDS 40.0%
TTTCCAGTAACAGGACACTT+AGG - chr7.1:85299929-85299948 None:intergenic 40.0%
TTTGTGAGGGACAATAAGGA+CGG - chr7.1:85300784-85300803 None:intergenic 40.0%
!!! GCTTATGGAGGTGTTTTATC+CGG + chr7.1:85301218-85301237 MS.gene92818:CDS 40.0%
!!! TCATTGCTTTTTCTCCCTGA+TGG + chr7.1:85301038-85301057 MS.gene92818:intron 40.0%
!!! TGGGTTTGTGTTGTTTTAGG+TGG + chr7.1:85299863-85299882 MS.gene92818:intron 40.0%
ACAAGAGGTCTAGATTGCCT+AGG + chr7.1:85301174-85301193 MS.gene92818:CDS 45.0%
ACTGTTAACAGGGCTTATGG+AGG + chr7.1:85301206-85301225 MS.gene92818:CDS 45.0%
AGTGCATGTCATGACCATCA+GGG - chr7.1:85301055-85301074 None:intergenic 45.0%
AGTGTCCTGTTACTGGAAAG+AGG + chr7.1:85299930-85299949 MS.gene92818:CDS 45.0%
CATAAGCCCTGTTAACAGTG+CGG - chr7.1:85301205-85301224 None:intergenic 45.0%
CTTAACAACACGATGCTGGT+TGG - chr7.1:85299710-85299729 None:intergenic 45.0%
GCTCTTCAACAAGGAAAGCA+CGG - chr7.1:85301354-85301373 None:intergenic 45.0%
GTGTCTTAACAACACGATGC+TGG - chr7.1:85299714-85299733 None:intergenic 45.0%
GTTACTGGAAAGAGGATCCA+AGG + chr7.1:85299938-85299957 MS.gene92818:CDS 45.0%
TACTGGAAAGAGGATCCAAG+GGG + chr7.1:85299940-85299959 MS.gene92818:CDS 45.0%
TGGATCCTCTTTCCAGTAAC+AGG - chr7.1:85299938-85299957 None:intergenic 45.0%
TTCAGAGACTCACATACCGT+AGG + chr7.1:85299662-85299681 MS.gene92818:CDS 45.0%
TTTATCCGGAGGAGCTGTTA+GGG + chr7.1:85301232-85301251 MS.gene92818:CDS 45.0%
! AACACGATGCTGGTTGGATT+TGG - chr7.1:85299704-85299723 None:intergenic 45.0%
! CATCGTGTTGTTAAGACACC+TGG + chr7.1:85299715-85299734 MS.gene92818:CDS 45.0%
! TTTTATCCGGAGGAGCTGTT+AGG + chr7.1:85301231-85301250 MS.gene92818:CDS 45.0%
!!! TATGGAGGTGTTTTATCCGG+AGG + chr7.1:85301221-85301240 MS.gene92818:CDS 45.0%
CGCACTGTTAACAGGGCTTA+TGG + chr7.1:85301203-85301222 MS.gene92818:CDS 50.0%
GAGTGCATGTCATGACCATC+AGG - chr7.1:85301056-85301075 None:intergenic 50.0%
GGACCTAAGTGTCCTGTTAC+TGG + chr7.1:85299923-85299942 MS.gene92818:CDS 50.0%
GTCTCAAGTGTGGAATCTGC+AGG - chr7.1:85301145-85301164 None:intergenic 50.0%
TGGCATAGCTGTGTCTCCTA+CGG - chr7.1:85299681-85299700 None:intergenic 50.0%
! ACGATGCTGGTTGGATTTGG+TGG - chr7.1:85299701-85299720 None:intergenic 50.0%
AAGCCCTGTTAACAGTGCGG+CGG - chr7.1:85301202-85301221 None:intergenic 55.0%
CCTTTCCCTAACAGCTCCTC+CGG - chr7.1:85301240-85301259 None:intergenic 55.0%
GAACCGCCGCACTGTTAACA+GGG + chr7.1:85301196-85301215 MS.gene92818:CDS 55.0%
TTAACAGTGCGGCGGTTCCT+AGG - chr7.1:85301194-85301213 None:intergenic 55.0%
CCGGAGGAGCTGTTAGGGAA+AGG + chr7.1:85301237-85301256 MS.gene92818:CDS 60.0%
GGAACCGCCGCACTGTTAAC+AGG + chr7.1:85301195-85301214 MS.gene92818:CDS 60.0%


Chromosome Type Strat End Strand Name
chr7.1 gene 85299658 85301438 85299658 ID=MS.gene92818
chr7.1 mRNA 85299658 85301438 85299658 ID=MS.gene92818.t1;Parent=MS.gene92818
chr7.1 exon 85299658 85299736 85299658 ID=MS.gene92818.t1.exon1;Parent=MS.gene92818.t1
chr7.1 CDS 85299658 85299736 85299658 ID=cds.MS.gene92818.t1;Parent=MS.gene92818.t1
chr7.1 exon 85299882 85299961 85299882 ID=MS.gene92818.t1.exon2;Parent=MS.gene92818.t1
chr7.1 CDS 85299882 85299961 85299882 ID=cds.MS.gene92818.t1;Parent=MS.gene92818.t1
chr7.1 exon 85301149 85301258 85301149 ID=MS.gene92818.t1.exon3;Parent=MS.gene92818.t1
chr7.1 CDS 85301149 85301258 85301149 ID=cds.MS.gene92818.t1;Parent=MS.gene92818.t1
chr7.1 exon 85301345 85301438 85301345 ID=MS.gene92818.t1.exon4;Parent=MS.gene92818.t1
chr7.1 CDS 85301345 85301438 85301345 ID=cds.MS.gene92818.t1;Parent=MS.gene92818.t1
Gene Sequence

>MS.gene92818

ATGGTTCAGAGACTCACATACCGTAGGAGACACAGCTATGCCACCAAATCCAACCAGCATCGTGTTGTTAAGACACCTGGTAATGTTCTTCTTTGTCAAATTGTTCTGTTTTTTCTTGTTCTAATCTATGTTATGATGTTTTTGATTTTTTCTTAAAAATTGCATTTTTTTGTTGTTGAAGTGTTTTTTGATTAATTTGTGAAAATGGGTTTGTGTTGTTTTAGGTGGAAAGTTGGTTTATCAGACAACTAAGAAGAGAGCTAGTGGACCTAAGTGTCCTGTTACTGGAAAGAGGATCCAAGGGGTATGAATCTATATGATATCATTTTTTTAGAAATTAATATGTTGGTTATACATAGGATCTGTGATTCATTGTGTTGATTCTATTTTTTTTGTTTGTTTGTACATGATGCTGTGGAAAAATGTAGATTGATATATGTGAGTTTATGATAGGACTTGTATTATTCATCAATTGGTTGTGTGAATTTGTCTGTGTTATGTAAAATAGGTGGAATATTAGGATTGTTTTGTGTTTTGCAATTATTAATGTTAATGGAATGCTGAGCTTATTATAAAATCAATTATTTGTGGTTTATTGAGCGAATGTTCTTACTTCAATGTTGGGTTTACTGTTTGATTGTTTGAAAATCCTAATATTTTCAATTTTCAAATCTATATTCCCACTAACATAGATGAATTCAATCAAATCATTTGTCTGTATTTTGATTAATGTTAATCGCTGAGCATATTATGAAATCAATTACTGTGTTTTTTTTAGTTAGCTTTTACTGTCTTTTATGCATGCTCCATCTGATTGAATATTGTGTTGTTTACGTCCCTATTACAGAAGAGATAGTTTGTTTTCTGCTTTAATTATGTGATGATTCAGTTTTTTTGAAGACGTAAATAACTTAATTCACCAATTCAGCTGTGTTTGTGAAGTGAATTAGTATAGTCTGTCTGTTTTTTTTGTAAGTCCATTTTTATTGAAGATTTGTTAGCCTATGTTTGAAACATGATAGATATAATAATCATTTCCAACTATGACCTTTGACGCTATCATCAATTTTATAATGACAAAATTAGATAGTTAGACTTAGATGCAATTCTTTTGAGATTGACACCGTCCTTATTGTCCCTCACAAAATTGTAGTATTAAAGATACCCTTTCTAAATTATTGGTGACTGTTTTTCTTATTTAGTTACAAACAACTAGAGCAGTGCCTATCACACAGCGTCTAAGTTTTGTCCATTGTATAATTTCAATGATGCAATTCTACAACATTGTGGAAGGAACATACAATGAATGTATTACTGTGTTACCCTGCATATAATCTTGACACTTTCCTACCTACTACTTTTAATTTTCATTCAAAGATTTCATTGCTTTTTCTCCCTGATGGTCATGACATGCACTCTTTATCTTTAGTTGCAAGTTGATCAATGTGTGTTCTCTTATCATATTTGTTCTGTTCTGGACTTGACCTGCAGATTCCACACTTGAGACCTACTGAATACAAGAGGTCTAGATTGCCTAGGAACCGCCGCACTGTTAACAGGGCTTATGGAGGTGTTTTATCCGGAGGAGCTGTTAGGGAAAGGTATGTTGCTCATAACATAATTACAGATTTATATTCAAAATTTTGAATATTGTAGTAAATTTTAATGCTCTTCAATCTTTGTGCAGAATTATCCGTGCTTTCCTTGTTGAAGAGCAAAAAATAGTTAAGAAAGTTTTGAAGATCCAGAAAACAAAAGAAAAGCAAGCAGCTAAGAGTTAA

Protein sequence

>MS.gene92818.t1

MVQRLTYRRRHSYATKSNQHRVVKTPGGKLVYQTTKKRASGPKCPVTGKRIQGIPHLRPTEYKRSRLPRNRRTVNRAYGGVLSGGAVRERIIRAFLVEEQKIVKKVLKIQKTKEKQAAKS