Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051628.t1 | AES59281.1 | 86.5 | 371 | 28 | 1 | 1 | 371 | 17 | 365 | 8.70E-187 | 662.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051628.t1 | Q3EBZ2 | 35.7 | 398 | 209 | 13 | 5 | 365 | 3 | 390 | 2.7e-48 | 194.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051628.t1 | G7IB97 | 86.5 | 371 | 28 | 1 | 1 | 371 | 17 | 365 | 6.3e-187 | 662.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051628 | MS.gene052239 | -0.801982 | 6.96E-49 | -1.69E-46 |
| MS.gene051628 | MS.gene053079 | 0.807051 | 6.10E-50 | -1.69E-46 |
| MS.gene051628 | MS.gene054651 | 0.803917 | 2.77E-49 | -1.69E-46 |
| MS.gene051628 | MS.gene057102 | 0.800276 | 1.56E-48 | -1.69E-46 |
| MS.gene051628 | MS.gene060587 | 0.801658 | 8.12E-49 | -1.69E-46 |
| MS.gene051628 | MS.gene061564 | 0.805716 | 1.17E-49 | -1.69E-46 |
| MS.gene051628 | MS.gene065559 | 0.809645 | 1.71E-50 | -1.69E-46 |
| MS.gene051628 | MS.gene067410 | 0.816865 | 4.44E-52 | -1.69E-46 |
| MS.gene051628 | MS.gene068006 | 0.800333 | 1.51E-48 | -1.69E-46 |
| MS.gene051628 | MS.gene070301 | 0.816996 | 4.15E-52 | -1.69E-46 |
| MS.gene051628 | MS.gene071247 | 0.803141 | 4.02E-49 | -1.69E-46 |
| MS.gene051628 | MS.gene071905 | 0.808801 | 2.59E-50 | -1.69E-46 |
| MS.gene051628 | MS.gene073727 | 0.80872 | 2.70E-50 | -1.69E-46 |
| MS.gene051628 | MS.gene074607 | 0.803204 | 3.90E-49 | -1.69E-46 |
| MS.gene051628 | MS.gene07876 | 0.808812 | 2.58E-50 | -1.69E-46 |
| MS.gene051628 | MS.gene21927 | 0.80274 | 4.86E-49 | -1.69E-46 |
| MS.gene051628 | MS.gene21929 | 0.804057 | 2.59E-49 | -1.69E-46 |
| MS.gene051628 | MS.gene24330 | 0.835178 | 1.95E-56 | -1.69E-46 |
| MS.gene051628 | MS.gene24375 | 0.800582 | 1.35E-48 | -1.69E-46 |
| MS.gene051628 | MS.gene26916 | 0.813526 | 2.45E-51 | -1.69E-46 |
| MS.gene051628 | MS.gene28611 | 0.813362 | 2.66E-51 | -1.69E-46 |
| MS.gene051628 | MS.gene29233 | 0.806058 | 9.89E-50 | -1.69E-46 |
| MS.gene051628 | MS.gene29631 | 0.800281 | 1.55E-48 | -1.69E-46 |
| MS.gene051628 | MS.gene32688 | 0.819573 | 1.08E-52 | -1.69E-46 |
| MS.gene051628 | MS.gene32976 | 0.81344 | 2.56E-51 | -1.69E-46 |
| MS.gene051628 | MS.gene34232 | 0.825957 | 3.53E-54 | -1.69E-46 |
| MS.gene051628 | MS.gene39074 | 0.801454 | 8.94E-49 | -1.69E-46 |
| MS.gene051628 | MS.gene40093 | 0.805501 | 1.30E-49 | -1.69E-46 |
| MS.gene051628 | MS.gene42653 | 0.811039 | 8.55E-51 | -1.69E-46 |
| MS.gene051628 | MS.gene43447 | 0.802599 | 5.20E-49 | -1.69E-46 |
| MS.gene051628 | MS.gene43449 | 0.810323 | 1.22E-50 | -1.69E-46 |
| MS.gene051628 | MS.gene44256 | 0.819964 | 8.81E-53 | -1.69E-46 |
| MS.gene051628 | MS.gene44356 | 0.800696 | 1.28E-48 | -1.69E-46 |
| MS.gene051628 | MS.gene48297 | 0.801084 | 1.06E-48 | -1.69E-46 |
| MS.gene051628 | MS.gene56742 | 0.809883 | 1.52E-50 | -1.69E-46 |
| MS.gene051628 | MS.gene58919 | 0.807147 | 5.82E-50 | -1.69E-46 |
| MS.gene051628 | MS.gene59241 | 0.814632 | 1.40E-51 | -1.69E-46 |
| MS.gene051628 | MS.gene59242 | 0.819419 | 1.17E-52 | -1.69E-46 |
| MS.gene051628 | MS.gene60827 | 0.8047 | 1.90E-49 | -1.69E-46 |
| MS.gene051628 | MS.gene62423 | 0.819054 | 1.42E-52 | -1.69E-46 |
| MS.gene051628 | MS.gene66973 | 0.806334 | 8.65E-50 | -1.69E-46 |
| MS.gene051628 | MS.gene67099 | 0.816258 | 6.07E-52 | -1.69E-46 |
| MS.gene051628 | MS.gene68653 | 0.80271 | 4.93E-49 | -1.69E-46 |
| MS.gene051628 | MS.gene68654 | 0.805148 | 1.54E-49 | -1.69E-46 |
| MS.gene051628 | MS.gene74972 | 0.806058 | 9.89E-50 | -1.69E-46 |
| MS.gene051628 | MS.gene75003 | 0.802775 | 4.78E-49 | -1.69E-46 |
| MS.gene051628 | MS.gene75990 | 0.805093 | 1.58E-49 | -1.69E-46 |
| MS.gene051628 | MS.gene79285 | 0.805877 | 1.08E-49 | -1.69E-46 |
| MS.gene051628 | MS.gene80201 | 0.818451 | 1.95E-52 | -1.69E-46 |
| MS.gene051628 | MS.gene80712 | 0.804078 | 2.57E-49 | -1.69E-46 |
| MS.gene051628 | MS.gene81149 | 0.80736 | 5.25E-50 | -1.69E-46 |
| MS.gene051628 | MS.gene81900 | 0.830638 | 2.62E-55 | -1.69E-46 |
| MS.gene051628 | MS.gene83529 | 0.804926 | 1.71E-49 | -1.69E-46 |
| MS.gene051628 | MS.gene83538 | 0.809226 | 2.10E-50 | -1.69E-46 |
| MS.gene051628 | MS.gene88147 | 0.803988 | 2.68E-49 | -1.69E-46 |
| MS.gene051628 | MS.gene89985 | 0.814035 | 1.89E-51 | -1.69E-46 |
| MS.gene051628 | MS.gene90260 | 0.805923 | 1.06E-49 | -1.69E-46 |
| MS.gene051628 | MS.gene90263 | 0.802549 | 5.32E-49 | -1.69E-46 |
| MS.gene051628 | MS.gene95728 | 0.816859 | 4.45E-52 | -1.69E-46 |
| MS.gene051628 | MS.gene96992 | 0.816908 | 4.34E-52 | -1.69E-46 |
| MS.gene051628 | MS.gene97784 | 0.815442 | 9.23E-52 | -1.69E-46 |
| MS.gene051628 | MS.gene99915 | 0.831028 | 2.11E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051628.t1 | MTR_1g016570 | 86.523 | 371 | 28 | 1 | 1 | 371 | 17 | 365 | 0.0 | 647 |
| MS.gene051628.t1 | MTR_3g030080 | 56.250 | 384 | 136 | 8 | 2 | 367 | 4 | 373 | 1.49e-132 | 384 |
| MS.gene051628.t1 | MTR_2g047180 | 56.771 | 384 | 131 | 13 | 5 | 370 | 4 | 370 | 3.30e-129 | 375 |
| MS.gene051628.t1 | MTR_3g029650 | 50.000 | 388 | 147 | 8 | 1 | 367 | 1 | 362 | 7.17e-108 | 333 |
| MS.gene051628.t1 | MTR_3g029650 | 44.697 | 396 | 178 | 12 | 1 | 367 | 368 | 751 | 4.35e-85 | 273 |
| MS.gene051628.t1 | MTR_3g030480 | 49.737 | 380 | 149 | 11 | 1 | 365 | 1 | 353 | 2.98e-104 | 311 |
| MS.gene051628.t1 | MTR_3g030380 | 49.737 | 380 | 156 | 9 | 1 | 367 | 1 | 358 | 4.13e-104 | 311 |
| MS.gene051628.t1 | MTR_6g018360 | 48.329 | 389 | 168 | 9 | 1 | 365 | 1 | 380 | 6.70e-104 | 313 |
| MS.gene051628.t1 | MTR_3g030440 | 49.077 | 379 | 151 | 11 | 2 | 365 | 3 | 354 | 8.38e-103 | 308 |
| MS.gene051628.t1 | MTR_3g030360 | 48.462 | 390 | 140 | 10 | 1 | 367 | 1 | 352 | 3.05e-100 | 301 |
| MS.gene051628.t1 | MTR_3g029670 | 47.132 | 401 | 164 | 11 | 1 | 367 | 1 | 387 | 3.56e-100 | 302 |
| MS.gene051628.t1 | MTR_6g018390 | 49.610 | 385 | 154 | 13 | 1 | 365 | 1 | 365 | 5.81e-100 | 301 |
| MS.gene051628.t1 | MTR_6g018315 | 48.813 | 379 | 151 | 13 | 16 | 365 | 1 | 365 | 6.99e-100 | 301 |
| MS.gene051628.t1 | MTR_3g029750 | 44.389 | 401 | 175 | 11 | 1 | 367 | 1 | 387 | 1.28e-93 | 285 |
| MS.gene051628.t1 | MTR_3g092000 | 44.656 | 421 | 166 | 13 | 2 | 373 | 4 | 406 | 8.70e-93 | 284 |
| MS.gene051628.t1 | MTR_1g049170 | 45.000 | 400 | 161 | 12 | 4 | 365 | 5 | 383 | 9.83e-93 | 283 |
| MS.gene051628.t1 | MTR_1g007260 | 45.932 | 381 | 171 | 13 | 1 | 361 | 18 | 383 | 7.78e-92 | 281 |
| MS.gene051628.t1 | MTR_6g018400 | 47.927 | 386 | 162 | 15 | 1 | 365 | 1 | 368 | 2.46e-91 | 279 |
| MS.gene051628.t1 | MTR_6g027100 | 45.823 | 395 | 164 | 12 | 1 | 365 | 1 | 375 | 1.43e-90 | 279 |
| MS.gene051628.t1 | MTR_3g091990 | 44.527 | 402 | 179 | 11 | 1 | 371 | 3 | 391 | 1.51e-89 | 276 |
| MS.gene051628.t1 | MTR_6g027040 | 45.316 | 395 | 166 | 12 | 1 | 365 | 1 | 375 | 2.84e-89 | 274 |
| MS.gene051628.t1 | MTR_3g029920 | 44.975 | 398 | 162 | 14 | 3 | 367 | 7 | 380 | 3.01e-88 | 271 |
| MS.gene051628.t1 | MTR_1g026850 | 45.213 | 376 | 177 | 12 | 3 | 365 | 4 | 363 | 5.96e-86 | 265 |
| MS.gene051628.t1 | MTR_3g029840 | 46.547 | 391 | 150 | 16 | 1 | 367 | 1 | 356 | 1.05e-84 | 261 |
| MS.gene051628.t1 | MTR_1g016540 | 69.519 | 187 | 51 | 4 | 188 | 371 | 1 | 184 | 1.77e-83 | 252 |
| MS.gene051628.t1 | MTR_3g030355 | 42.336 | 411 | 174 | 15 | 2 | 367 | 3 | 395 | 5.83e-82 | 256 |
| MS.gene051628.t1 | MTR_3g029950 | 45.226 | 398 | 157 | 17 | 1 | 367 | 1 | 368 | 8.93e-80 | 249 |
| MS.gene051628.t1 | MTR_3g030050 | 38.095 | 420 | 188 | 11 | 4 | 365 | 15 | 420 | 2.79e-78 | 247 |
| MS.gene051628.t1 | MTR_3g029620 | 43.257 | 393 | 178 | 16 | 4 | 369 | 2 | 376 | 7.48e-77 | 242 |
| MS.gene051628.t1 | MTR_3g029640 | 43.511 | 393 | 159 | 17 | 4 | 365 | 3 | 363 | 5.07e-76 | 239 |
| MS.gene051628.t1 | MTR_3g030560 | 44.246 | 391 | 170 | 16 | 3 | 365 | 2 | 372 | 1.10e-75 | 239 |
| MS.gene051628.t1 | MTR_3g058460 | 39.687 | 383 | 195 | 12 | 1 | 365 | 11 | 375 | 8.23e-70 | 224 |
| MS.gene051628.t1 | MTR_3g029870 | 41.582 | 392 | 147 | 15 | 1 | 367 | 1 | 335 | 4.40e-69 | 221 |
| MS.gene051628.t1 | MTR_3g029800 | 40.360 | 389 | 169 | 15 | 4 | 365 | 3 | 355 | 6.32e-66 | 213 |
| MS.gene051628.t1 | MTR_3g029900 | 46.057 | 317 | 130 | 12 | 61 | 365 | 170 | 457 | 8.70e-65 | 214 |
| MS.gene051628.t1 | MTR_3g047980 | 42.216 | 379 | 152 | 16 | 5 | 365 | 30 | 359 | 1.01e-58 | 195 |
| MS.gene051628.t1 | MTR_7g005990 | 38.579 | 394 | 176 | 16 | 3 | 367 | 2 | 358 | 2.59e-57 | 191 |
| MS.gene051628.t1 | MTR_6g018370 | 41.009 | 317 | 113 | 8 | 3 | 304 | 2 | 259 | 1.03e-56 | 186 |
| MS.gene051628.t1 | MTR_3g029820 | 39.594 | 394 | 133 | 16 | 5 | 367 | 9 | 328 | 4.89e-55 | 184 |
| MS.gene051628.t1 | MTR_3g037710 | 38.408 | 289 | 141 | 9 | 103 | 373 | 1 | 270 | 1.98e-50 | 170 |
| MS.gene051628.t1 | MTR_5g042230 | 51.913 | 183 | 81 | 3 | 173 | 354 | 12 | 188 | 4.40e-48 | 162 |
| MS.gene051628.t1 | MTR_1g007250 | 52.047 | 171 | 71 | 5 | 188 | 358 | 1 | 160 | 1.21e-41 | 144 |
| MS.gene051628.t1 | MTR_1g007220 | 43.627 | 204 | 76 | 3 | 156 | 358 | 2 | 167 | 4.52e-41 | 142 |
| MS.gene051628.t1 | MTR_8g074610 | 28.426 | 394 | 226 | 11 | 5 | 365 | 25 | 395 | 2.62e-38 | 142 |
| MS.gene051628.t1 | MTR_5g078450 | 52.817 | 142 | 45 | 1 | 231 | 372 | 5 | 124 | 3.40e-38 | 133 |
| MS.gene051628.t1 | MTR_2g041510 | 30.833 | 360 | 176 | 12 | 5 | 320 | 6 | 336 | 3.01e-31 | 122 |
| MS.gene051628.t1 | MTR_2g041510 | 30.833 | 360 | 176 | 12 | 5 | 320 | 26 | 356 | 4.77e-31 | 122 |
| MS.gene051628.t1 | MTR_8g074640 | 30.189 | 371 | 208 | 12 | 5 | 341 | 6 | 359 | 3.92e-30 | 119 |
| MS.gene051628.t1 | MTR_4g022880 | 43.478 | 138 | 70 | 2 | 237 | 368 | 39 | 174 | 3.71e-26 | 103 |
| MS.gene051628.t1 | MTR_6g092570 | 28.011 | 357 | 210 | 13 | 5 | 338 | 8 | 340 | 9.88e-24 | 101 |
| MS.gene051628.t1 | MTR_1g007200 | 50.000 | 104 | 39 | 3 | 249 | 350 | 1 | 93 | 6.93e-20 | 85.1 |
| MS.gene051628.t1 | MTR_5g079830 | 25.595 | 336 | 206 | 10 | 5 | 318 | 6 | 319 | 1.05e-19 | 90.1 |
| MS.gene051628.t1 | MTR_8g032080 | 48.485 | 99 | 44 | 3 | 116 | 208 | 3 | 100 | 4.31e-19 | 87.4 |
| MS.gene051628.t1 | MTR_8g035995 | 47.475 | 99 | 45 | 2 | 197 | 295 | 1 | 92 | 7.74e-17 | 77.4 |
| MS.gene051628.t1 | MTR_6g009100 | 41.912 | 136 | 49 | 5 | 4 | 129 | 3 | 118 | 2.22e-16 | 75.1 |
| MS.gene051628.t1 | MTR_1g007230 | 48.235 | 85 | 35 | 3 | 2 | 78 | 5 | 88 | 2.42e-14 | 68.6 |
| MS.gene051628.t1 | MTR_6g009620 | 43.617 | 94 | 39 | 5 | 83 | 167 | 38 | 126 | 3.36e-14 | 69.3 |
| MS.gene051628.t1 | MTR_3g114990 | 23.873 | 377 | 240 | 14 | 5 | 348 | 23 | 385 | 1.20e-13 | 72.0 |
| MS.gene051628.t1 | MTR_6g018420 | 72.973 | 37 | 10 | 0 | 1 | 37 | 1 | 37 | 6.83e-13 | 64.3 |
| MS.gene051628.t1 | MTR_6g075925 | 69.767 | 43 | 13 | 0 | 1 | 43 | 1 | 43 | 1.14e-12 | 63.5 |
| MS.gene051628.t1 | MTR_4g088925 | 24.581 | 358 | 197 | 13 | 16 | 332 | 37 | 362 | 4.58e-12 | 67.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051628.t1 | AT2G17030 | 35.516 | 397 | 211 | 13 | 5 | 365 | 3 | 390 | 2.03e-55 | 187 |
| MS.gene051628.t1 | AT2G17036 | 30.941 | 404 | 214 | 14 | 5 | 365 | 3 | 384 | 2.74e-42 | 152 |
| MS.gene051628.t1 | AT2G17036 | 30.941 | 404 | 214 | 14 | 5 | 365 | 2 | 383 | 2.75e-42 | 152 |
| MS.gene051628.t1 | AT5G24040 | 27.105 | 380 | 243 | 18 | 6 | 365 | 4 | 369 | 3.28e-24 | 102 |
| MS.gene051628.t1 | AT5G60060 | 27.733 | 375 | 237 | 16 | 4 | 355 | 9 | 372 | 3.88e-22 | 97.1 |
| MS.gene051628.t1 | AT3G25750 | 27.297 | 381 | 220 | 16 | 1 | 361 | 1 | 344 | 1.21e-21 | 95.1 |
| MS.gene051628.t1 | AT3G25750 | 25.974 | 385 | 234 | 15 | 1 | 361 | 1 | 358 | 9.90e-21 | 92.8 |
| MS.gene051628.t1 | AT1G65770 | 27.299 | 348 | 196 | 12 | 4 | 320 | 2 | 323 | 2.26e-18 | 85.9 |
| MS.gene051628.t1 | AT2G26160 | 24.128 | 344 | 213 | 12 | 1 | 320 | 1 | 320 | 1.61e-14 | 74.3 |
| MS.gene051628.t1 | AT1G65740 | 24.930 | 357 | 203 | 13 | 5 | 320 | 3 | 335 | 1.22e-11 | 65.9 |
Find 82 sgRNAs with CRISPR-Local
Find 102 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAACTTTATGTACTTGAATT+AGG | 0.191945 | 1.3:+5720045 | MS.gene051628:CDS |
| TTGATGAGAAGGAGAGTAAA+TGG | 0.206024 | 1.3:+5720508 | MS.gene051628:CDS |
| AAACCAACGGAGATGAATTT+TGG | 0.212262 | 1.3:+5720208 | MS.gene051628:CDS |
| ATGGGTTGATTTGATGAGTT+TGG | 0.251492 | 1.3:+5720527 | MS.gene051628:CDS |
| TTAACCACAAGTTCATATTT+AGG | 0.261634 | 1.3:-5720366 | None:intergenic |
| ATTTGATGAGTTTGGGAGAT+AGG | 0.289011 | 1.3:+5720535 | MS.gene051628:CDS |
| AAATATGAACTTGTGGTTAA+TGG | 0.323243 | 1.3:+5720369 | MS.gene051628:CDS |
| GAGGAGTGGGTGTAAGAGTT+TGG | 0.328183 | 1.3:-5719971 | None:intergenic |
| GTGGACATTTATGAGTGTTT+AGG | 0.334870 | 1.3:+5720438 | MS.gene051628:CDS |
| GATCGCATGACTTCGATAAC+TGG | 0.340536 | 1.3:+5720334 | MS.gene051628:CDS |
| CCAATTCCATCAATCATATA+AGG | 0.345857 | 1.3:-5720288 | None:intergenic |
| CGGACAATTATTGTTGGACT+TGG | 0.356140 | 1.3:+5720312 | MS.gene051628:CDS |
| TTCACCTTTGTCTGATTGTC+CGG | 0.356602 | 1.3:+5720719 | MS.gene051628:CDS |
| GTTGTTGATGGGAGATTGAT+AGG | 0.367917 | 1.3:-5719845 | None:intergenic |
| TGGAAACTTGAGGGTTAGAT+TGG | 0.369886 | 1.3:-5719818 | None:intergenic |
| ATCAGATGTAGAAGCTCCTT+TGG | 0.370393 | 1.3:-5719712 | None:intergenic |
| TTTCAACGAGACTGATTTCT+TGG | 0.370571 | 1.3:-5720187 | None:intergenic |
| GCAAGATAACATACTCTGTT+AGG | 0.372490 | 1.3:-5720795 | None:intergenic |
| CAGATTTGTGTGTTTCTAAA+GGG | 0.375553 | 1.3:+5720601 | MS.gene051628:CDS |
| CAGAACAAGTTAAAATATTC+CGG | 0.384086 | 1.3:-5720738 | None:intergenic |
| TTGAGGGTTAGATTGGGAAT+TGG | 0.401176 | 1.3:-5719811 | None:intergenic |
| CCTTATATGATTGATGGAAT+TGG | 0.407615 | 1.3:+5720288 | MS.gene051628:CDS |
| TCAGATTTGTGTGTTTCTAA+AGG | 0.427259 | 1.3:+5720600 | MS.gene051628:CDS |
| TACTGATATGTCTATGTACT+TGG | 0.428125 | 1.3:+5720239 | MS.gene051628:CDS |
| TCAGGGTAAACATAGTCATA+TGG | 0.438455 | 1.3:-5720102 | None:intergenic |
| TTGTTGATGGGAGATTGATA+GGG | 0.442668 | 1.3:-5719844 | None:intergenic |
| TGATGAGAAGGAGAGTAAAT+GGG | 0.446912 | 1.3:+5720509 | MS.gene051628:CDS |
| TGTATCTTTGACTTGGATCA+AGG | 0.447583 | 1.3:+5720690 | MS.gene051628:CDS |
| GGGCGGCCTTATATGATTGA+TGG | 0.452306 | 1.3:+5720282 | MS.gene051628:CDS |
| AACCACCACTTCAACGCCCT+TGG | 0.454393 | 1.3:+5719914 | MS.gene051628:CDS |
| TGGGTTGATTTGATGAGTTT+GGG | 0.459847 | 1.3:+5720528 | MS.gene051628:CDS |
| GAGTTCAACGTCGAGCCGTT+GGG | 0.460050 | 1.3:-5719737 | None:intergenic |
| GGTTAGATTGGGAATTGGTA+AGG | 0.460418 | 1.3:-5719806 | None:intergenic |
| TGTGTTTAGGTTTGATGAGA+AGG | 0.463451 | 1.3:+5720497 | MS.gene051628:CDS |
| AAATTGAGTGGTGTTGTTGA+TGG | 0.466840 | 1.3:-5719857 | None:intergenic |
| TTTGATGAGTTTGGGAGATA+GGG | 0.474756 | 1.3:+5720536 | MS.gene051628:CDS |
| ATAGGGGTGTGGAAACTTGA+GGG | 0.481501 | 1.3:-5719827 | None:intergenic |
| ATTGGTCGGACAATTATTGT+TGG | 0.482444 | 1.3:+5720306 | MS.gene051628:CDS |
| TCAGATGTAGAAGCTCCTTT+GGG | 0.486819 | 1.3:-5719711 | None:intergenic |
| CGAGTTCAACGTCGAGCCGT+TGG | 0.490351 | 1.3:-5719738 | None:intergenic |
| CATCAATGGCAGAGTCAGAC+TGG | 0.493551 | 1.3:+5719680 | None:intergenic |
| ACACCAAAATTCATCTCCGT+TGG | 0.512605 | 1.3:-5720211 | None:intergenic |
| TCCGATCAACTTGTTCCGCA+TGG | 0.522258 | 1.3:+5719773 | MS.gene051628:CDS |
| TGGGAACTGTATCTTTGACT+TGG | 0.526556 | 1.3:+5720683 | MS.gene051628:CDS |
| AACCAAGGGCGTTGAAGTGG+TGG | 0.538102 | 1.3:-5719916 | None:intergenic |
| ATGGGAGATTGATAGGGGTG+TGG | 0.538525 | 1.3:-5719838 | None:intergenic |
| TGAAGGCGCGTTGCTGCGAG+TGG | 0.540250 | 1.3:+5720419 | MS.gene051628:CDS |
| TATGAACTTGTGGTTAATGG+TGG | 0.543129 | 1.3:+5720372 | MS.gene051628:CDS |
| GATAGGGGTGTGGAAACTTG+AGG | 0.545526 | 1.3:-5719828 | None:intergenic |
| AATTGAGTGGTGTTGTTGAT+GGG | 0.546505 | 1.3:-5719856 | None:intergenic |
| GGAAACTTGAGGGTTAGATT+GGG | 0.553565 | 1.3:-5719817 | None:intergenic |
| ATCAACCAAGGGCGTTGAAG+TGG | 0.559025 | 1.3:-5719919 | None:intergenic |
| ATGATTGATATGAGAGGAGT+GGG | 0.567475 | 1.3:-5719984 | None:intergenic |
| TGAACTTGTGGTTAATGGTG+GGG | 0.569319 | 1.3:+5720374 | MS.gene051628:CDS |
| TGTTGATGGGAGATTGATAG+GGG | 0.579514 | 1.3:-5719843 | None:intergenic |
| GATGAGATGATTGATATGAG+AGG | 0.579863 | 1.3:-5719990 | None:intergenic |
| GAACTTGTGGTTAATGGTGG+GGG | 0.582058 | 1.3:+5720375 | MS.gene051628:CDS |
| ATGAACTTGTGGTTAATGGT+GGG | 0.584883 | 1.3:+5720373 | MS.gene051628:CDS |
| GAGTAATTCTGATCAACCAA+GGG | 0.589964 | 1.3:-5719930 | None:intergenic |
| TTGTCGATCCACTCTGTAGG+TGG | 0.596783 | 1.3:-5720762 | None:intergenic |
| ACCACCTAAATATGAACTTG+TGG | 0.597935 | 1.3:+5720362 | MS.gene051628:CDS |
| AGACTGGTCTTCACTCCCAA+AGG | 0.598232 | 1.3:+5719696 | MS.gene051628:CDS |
| TCACTAAAATCGAGAGCGTG+AGG | 0.598776 | 1.3:-5720024 | None:intergenic |
| AGAGCGTGAGGGAAAAGAAG+AGG | 0.600055 | 1.3:-5720012 | None:intergenic |
| ACTGATATGTCTATGTACTT+GGG | 0.602971 | 1.3:+5720240 | MS.gene051628:CDS |
| ACCACAAGTTCATATTTAGG+TGG | 0.605610 | 1.3:-5720363 | None:intergenic |
| GTTGAAGTGGTGGTTTGATG+AGG | 0.606138 | 1.3:-5719906 | None:intergenic |
| TGAGTAATTCTGATCAACCA+AGG | 0.612564 | 1.3:-5719931 | None:intergenic |
| CACTAAAATCGAGAGCGTGA+GGG | 0.616468 | 1.3:-5720023 | None:intergenic |
| CATTTGTCGATCCACTCTGT+AGG | 0.617177 | 1.3:-5720765 | None:intergenic |
| TCAGTCTCGTTGAAAACCAA+CGG | 0.621836 | 1.3:+5720195 | MS.gene051628:CDS |
| GCCATGCGGAACAAGTTGAT+CGG | 0.628441 | 1.3:-5719774 | None:intergenic |
| GGAGAGAGAGTGAAATTGAG+TGG | 0.633283 | 1.3:-5719869 | None:intergenic |
| TTCTACATCTGATATCCCAA+CGG | 0.636046 | 1.3:+5719722 | MS.gene051628:CDS |
| ATATGATTGATGGAATTGGT+CGG | 0.636318 | 1.3:+5720292 | MS.gene051628:CDS |
| ATAAACGTGCCAATAACAAG+AGG | 0.645995 | 1.3:-5720159 | None:intergenic |
| GATGATTGATATGAGAGGAG+TGG | 0.652451 | 1.3:-5719985 | None:intergenic |
| AAATTCTTAGTGGAGAGTGA+AGG | 0.653477 | 1.3:+5720402 | MS.gene051628:CDS |
| AGAATTACTCAAAACCCTAA+CGG | 0.674771 | 1.3:+5719943 | MS.gene051628:CDS |
| TATTCCGGACAATCAGACAA+AGG | 0.675553 | 1.3:-5720723 | None:intergenic |
| TGTTCTGGCCACCTACAGAG+TGG | 0.683477 | 1.3:+5720754 | MS.gene051628:CDS |
| TAAGGAAGAAGAGCGCCATG+CGG | 0.759001 | 1.3:-5719788 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTGTAAAAACATGCAATA+TGG | + | chr1.3:5720663-5720682 | MS.gene051628:CDS | 20.0% |
| !! | ATTGTAAAAACATGCAATAT+GGG | + | chr1.3:5720664-5720683 | MS.gene051628:CDS | 20.0% |
| !!! | TTTTAAGGATTAATGTGTTT+AGG | + | chr1.3:5720484-5720503 | MS.gene051628:CDS | 20.0% |
| ! | AAATATGAACTTGTGGTTAA+TGG | + | chr1.3:5720369-5720388 | MS.gene051628:CDS | 25.0% |
| ! | AAGATTATCTAATCTGACAA+CGG | - | chr1.3:5720838-5720857 | None:intergenic | 25.0% |
| ! | CAGAACAAGTTAAAATATTC+CGG | - | chr1.3:5720741-5720760 | None:intergenic | 25.0% |
| ! | GAACTTTATGTACTTGAATT+AGG | + | chr1.3:5720045-5720064 | MS.gene051628:CDS | 25.0% |
| ! | TTAACCACAAGTTCATATTT+AGG | - | chr1.3:5720369-5720388 | None:intergenic | 25.0% |
| !!! | TGTTTAGGTTTTGATTACTT+CGG | + | chr1.3:5720453-5720472 | MS.gene051628:CDS | 25.0% |
| ACTGATATGTCTATGTACTT+GGG | + | chr1.3:5720240-5720259 | MS.gene051628:CDS | 30.0% | |
| AGAATTACTCAAAACCCTAA+CGG | + | chr1.3:5719943-5719962 | MS.gene051628:CDS | 30.0% | |
| ATATGATTGATGGAATTGGT+CGG | + | chr1.3:5720292-5720311 | MS.gene051628:CDS | 30.0% | |
| CCAATTCCATCAATCATATA+AGG | - | chr1.3:5720291-5720310 | None:intergenic | 30.0% | |
| CCTTATATGATTGATGGAAT+TGG | + | chr1.3:5720288-5720307 | MS.gene051628:CDS | 30.0% | |
| TACTGATATGTCTATGTACT+TGG | + | chr1.3:5720239-5720258 | MS.gene051628:CDS | 30.0% | |
| ! | ACTTCGGTAAGAATGTTTTA+AGG | + | chr1.3:5720469-5720488 | MS.gene051628:CDS | 30.0% |
| ! | CAGATTTGTGTGTTTCTAAA+GGG | + | chr1.3:5720601-5720620 | MS.gene051628:CDS | 30.0% |
| ! | GAAAAAAAGCCTCTTGTTAT+TGG | + | chr1.3:5720150-5720169 | MS.gene051628:CDS | 30.0% |
| ! | TCAGATTTGTGTGTTTCTAA+AGG | + | chr1.3:5720600-5720619 | MS.gene051628:CDS | 30.0% |
| !!! | CGGAATATTTTAACTTGTTC+TGG | + | chr1.3:5720739-5720758 | MS.gene051628:CDS | 30.0% |
| AAATTCTTAGTGGAGAGTGA+AGG | + | chr1.3:5720402-5720421 | MS.gene051628:CDS | 35.0% | |
| ACCACAAGTTCATATTTAGG+TGG | - | chr1.3:5720366-5720385 | None:intergenic | 35.0% | |
| ACCACCTAAATATGAACTTG+TGG | + | chr1.3:5720362-5720381 | MS.gene051628:CDS | 35.0% | |
| ATAAACGTGCCAATAACAAG+AGG | - | chr1.3:5720162-5720181 | None:intergenic | 35.0% | |
| ATGAACTTGTGGTTAATGGT+GGG | + | chr1.3:5720373-5720392 | MS.gene051628:CDS | 35.0% | |
| ATGATTGATATGAGAGGAGT+GGG | - | chr1.3:5719987-5720006 | None:intergenic | 35.0% | |
| ATTGGTCGGACAATTATTGT+TGG | + | chr1.3:5720306-5720325 | MS.gene051628:CDS | 35.0% | |
| ATTTGATGAGTTTGGGAGAT+AGG | + | chr1.3:5720535-5720554 | MS.gene051628:CDS | 35.0% | |
| GAGTAATTCTGATCAACCAA+GGG | - | chr1.3:5719933-5719952 | None:intergenic | 35.0% | |
| GATGAGATGATTGATATGAG+AGG | - | chr1.3:5719993-5720012 | None:intergenic | 35.0% | |
| GCAAGATAACATACTCTGTT+AGG | - | chr1.3:5720798-5720817 | None:intergenic | 35.0% | |
| GTGGACATTTATGAGTGTTT+AGG | + | chr1.3:5720438-5720457 | MS.gene051628:CDS | 35.0% | |
| TATGAACTTGTGGTTAATGG+TGG | + | chr1.3:5720372-5720391 | MS.gene051628:CDS | 35.0% | |
| TCAGGGTAAACATAGTCATA+TGG | - | chr1.3:5720105-5720124 | None:intergenic | 35.0% | |
| TGAGTAATTCTGATCAACCA+AGG | - | chr1.3:5719934-5719953 | None:intergenic | 35.0% | |
| TGATGAGAAGGAGAGTAAAT+GGG | + | chr1.3:5720509-5720528 | MS.gene051628:CDS | 35.0% | |
| TTCTACATCTGATATCCCAA+CGG | + | chr1.3:5719722-5719741 | MS.gene051628:CDS | 35.0% | |
| TTGATGAGAAGGAGAGTAAA+TGG | + | chr1.3:5720508-5720527 | MS.gene051628:CDS | 35.0% | |
| TTTGATGAGTTTGGGAGATA+GGG | + | chr1.3:5720536-5720555 | MS.gene051628:CDS | 35.0% | |
| ! | AAACCAACGGAGATGAATTT+TGG | + | chr1.3:5720208-5720227 | MS.gene051628:CDS | 35.0% |
| ! | TGTGTTTAGGTTTGATGAGA+AGG | + | chr1.3:5720497-5720516 | MS.gene051628:CDS | 35.0% |
| ! | TTGTTGATGGGAGATTGATA+GGG | - | chr1.3:5719847-5719866 | None:intergenic | 35.0% |
| ! | TTTCAACGAGACTGATTTCT+TGG | - | chr1.3:5720190-5720209 | None:intergenic | 35.0% |
| !! | AAATTGAGTGGTGTTGTTGA+TGG | - | chr1.3:5719860-5719879 | None:intergenic | 35.0% |
| !! | AATTGAGTGGTGTTGTTGAT+GGG | - | chr1.3:5719859-5719878 | None:intergenic | 35.0% |
| !! | ATGGGTTGATTTGATGAGTT+TGG | + | chr1.3:5720527-5720546 | MS.gene051628:CDS | 35.0% |
| !! | TGGGTTGATTTGATGAGTTT+GGG | + | chr1.3:5720528-5720547 | MS.gene051628:CDS | 35.0% |
| !! | TGTATCTTTGACTTGGATCA+AGG | + | chr1.3:5720690-5720709 | MS.gene051628:CDS | 35.0% |
| !!! | AAGAGTTTGGTTTTACCGTT+AGG | - | chr1.3:5719961-5719980 | None:intergenic | 35.0% |
| !!! | AGAGTTTGGTTTTACCGTTA+GGG | - | chr1.3:5719960-5719979 | None:intergenic | 35.0% |
| !!! | GAGATAGGGTTTTGTTTTTG+GGG | + | chr1.3:5720550-5720569 | MS.gene051628:CDS | 35.0% |
| !!! | GGAGATAGGGTTTTGTTTTT+GGG | + | chr1.3:5720549-5720568 | MS.gene051628:CDS | 35.0% |
| ACACCAAAATTCATCTCCGT+TGG | - | chr1.3:5720214-5720233 | None:intergenic | 40.0% | |
| ATCAGATGTAGAAGCTCCTT+TGG | - | chr1.3:5719715-5719734 | None:intergenic | 40.0% | |
| CGGACAATTATTGTTGGACT+TGG | + | chr1.3:5720312-5720331 | MS.gene051628:CDS | 40.0% | |
| GATGATTGATATGAGAGGAG+TGG | - | chr1.3:5719988-5720007 | None:intergenic | 40.0% | |
| GGAAACTTGAGGGTTAGATT+GGG | - | chr1.3:5719820-5719839 | None:intergenic | 40.0% | |
| GGTTAGATTGGGAATTGGTA+AGG | - | chr1.3:5719809-5719828 | None:intergenic | 40.0% | |
| TATTCCGGACAATCAGACAA+AGG | - | chr1.3:5720726-5720745 | None:intergenic | 40.0% | |
| TCAGATGTAGAAGCTCCTTT+GGG | - | chr1.3:5719714-5719733 | None:intergenic | 40.0% | |
| TCAGTCTCGTTGAAAACCAA+CGG | + | chr1.3:5720195-5720214 | MS.gene051628:CDS | 40.0% | |
| TGAACTTGTGGTTAATGGTG+GGG | + | chr1.3:5720374-5720393 | MS.gene051628:CDS | 40.0% | |
| TGGAAACTTGAGGGTTAGAT+TGG | - | chr1.3:5719821-5719840 | None:intergenic | 40.0% | |
| TGGGAACTGTATCTTTGACT+TGG | + | chr1.3:5720683-5720702 | MS.gene051628:CDS | 40.0% | |
| TGGGGGCAAAAAATTCTTAG+TGG | + | chr1.3:5720392-5720411 | MS.gene051628:CDS | 40.0% | |
| TTCACCTTTGTCTGATTGTC+CGG | + | chr1.3:5720719-5720738 | MS.gene051628:CDS | 40.0% | |
| TTGAGGGTTAGATTGGGAAT+TGG | - | chr1.3:5719814-5719833 | None:intergenic | 40.0% | |
| ! | GAGACATCTGCGTTTTCAAA+GGG | + | chr1.3:5720262-5720281 | MS.gene051628:CDS | 40.0% |
| ! | GTTGTTGATGGGAGATTGAT+AGG | - | chr1.3:5719848-5719867 | None:intergenic | 40.0% |
| ! | TGACAGCAACGATCTTTTCA+GGG | - | chr1.3:5720122-5720141 | None:intergenic | 40.0% |
| ! | TGTTGATGGGAGATTGATAG+GGG | - | chr1.3:5719846-5719865 | None:intergenic | 40.0% |
| !! | GATGAGGAAAAAAGAGCGTT+TGG | - | chr1.3:5719893-5719912 | None:intergenic | 40.0% |
| !!! | AGGGTTTTGTTTTTGGGGAA+TGG | + | chr1.3:5720555-5720574 | MS.gene051628:CDS | 40.0% |
| !!! | GGGAGATAGGGTTTTGTTTT+TGG | + | chr1.3:5720548-5720567 | MS.gene051628:CDS | 40.0% |
| CATTTGTCGATCCACTCTGT+AGG | - | chr1.3:5720768-5720787 | None:intergenic | 45.0% | |
| GAACTTGTGGTTAATGGTGG+GGG | + | chr1.3:5720375-5720394 | MS.gene051628:CDS | 45.0% | |
| GATCGCATGACTTCGATAAC+TGG | + | chr1.3:5720334-5720353 | MS.gene051628:CDS | 45.0% | |
| GGAGAGAGAGTGAAATTGAG+TGG | - | chr1.3:5719872-5719891 | None:intergenic | 45.0% | |
| GTTGAAGTGGTGGTTTGATG+AGG | - | chr1.3:5719909-5719928 | None:intergenic | 45.0% | |
| ! | ACATCTGCGTTTTCAAAGGG+CGG | + | chr1.3:5720265-5720284 | MS.gene051628:CDS | 45.0% |
| ! | ATAGGGGTGTGGAAACTTGA+GGG | - | chr1.3:5719830-5719849 | None:intergenic | 45.0% |
| ! | CACTAAAATCGAGAGCGTGA+GGG | - | chr1.3:5720026-5720045 | None:intergenic | 45.0% |
| ! | GGAGACATCTGCGTTTTCAA+AGG | + | chr1.3:5720261-5720280 | MS.gene051628:CDS | 45.0% |
| ! | GTGACAGCAACGATCTTTTC+AGG | - | chr1.3:5720123-5720142 | None:intergenic | 45.0% |
| ! | TCACTAAAATCGAGAGCGTG+AGG | - | chr1.3:5720027-5720046 | None:intergenic | 45.0% |
| AGAGCGTGAGGGAAAAGAAG+AGG | - | chr1.3:5720015-5720034 | None:intergenic | 50.0% | |
| ATGGGAGATTGATAGGGGTG+TGG | - | chr1.3:5719841-5719860 | None:intergenic | 50.0% | |
| GAGGAGTGGGTGTAAGAGTT+TGG | - | chr1.3:5719974-5719993 | None:intergenic | 50.0% | |
| TTGTCGATCCACTCTGTAGG+TGG | - | chr1.3:5720765-5720784 | None:intergenic | 50.0% | |
| ! | GATAGGGGTGTGGAAACTTG+AGG | - | chr1.3:5719831-5719850 | None:intergenic | 50.0% |
| ! | GGGCGGCCTTATATGATTGA+TGG | + | chr1.3:5720282-5720301 | MS.gene051628:CDS | 50.0% |
| ! | TCCGATCAACTTGTTCCGCA+TGG | + | chr1.3:5719773-5719792 | MS.gene051628:CDS | 50.0% |
| !! | AGACTGGTCTTCACTCCCAA+AGG | + | chr1.3:5719696-5719715 | MS.gene051628:CDS | 50.0% |
| !! | ATCAACCAAGGGCGTTGAAG+TGG | - | chr1.3:5719922-5719941 | None:intergenic | 50.0% |
| !! | GCCATGCGGAACAAGTTGAT+CGG | - | chr1.3:5719777-5719796 | None:intergenic | 50.0% |
| !! | TAAGGAAGAAGAGCGCCATG+CGG | - | chr1.3:5719791-5719810 | None:intergenic | 50.0% |
| AACCACCACTTCAACGCCCT+TGG | + | chr1.3:5719914-5719933 | MS.gene051628:CDS | 55.0% | |
| GAGTTCAACGTCGAGCCGTT+GGG | - | chr1.3:5719740-5719759 | None:intergenic | 55.0% | |
| TGTTCTGGCCACCTACAGAG+TGG | + | chr1.3:5720754-5720773 | MS.gene051628:CDS | 55.0% | |
| !! | AACCAAGGGCGTTGAAGTGG+TGG | - | chr1.3:5719919-5719938 | None:intergenic | 55.0% |
| CGAGTTCAACGTCGAGCCGT+TGG | - | chr1.3:5719741-5719760 | None:intergenic | 60.0% | |
| ! | TGAAGGCGCGTTGCTGCGAG+TGG | + | chr1.3:5720419-5720438 | MS.gene051628:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 5719685 | 5720880 | 5719685 | ID=MS.gene051628 |
| chr1.3 | mRNA | 5719685 | 5720880 | 5719685 | ID=MS.gene051628.t1;Parent=MS.gene051628 |
| chr1.3 | exon | 5719685 | 5720803 | 5719685 | ID=MS.gene051628.t1.exon1;Parent=MS.gene051628.t1 |
| chr1.3 | CDS | 5719685 | 5720803 | 5719685 | ID=cds.MS.gene051628.t1;Parent=MS.gene051628.t1 |
| chr1.3 | exon | 5720875 | 5720880 | 5720875 | ID=MS.gene051628.t1.exon2;Parent=MS.gene051628.t1 |
| chr1.3 | CDS | 5720875 | 5720880 | 5720875 | ID=cds.MS.gene051628.t1;Parent=MS.gene051628.t1 |
| Gene Sequence |
| Protein sequence |
