Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047815.01 | MsG0180002217.01 | 0.804065 | 2.583039e-49 | 1.319199e-46 |
| MsG0080048797.01 | MsG0180002217.01 | 0.813400 | 2.609386e-51 | 1.702330e-48 |
| MsG0180001293.01 | MsG0180002217.01 | 0.817162 | 3.804539e-52 | 2.749807e-49 |
| MsG0180001719.01 | MsG0180002217.01 | 0.807439 | 5.052701e-50 | 2.814307e-47 |
| MsG0180002134.01 | MsG0180002217.01 | 0.810731 | 9.963107e-51 | 6.052997e-48 |
| MsG0180002217.01 | MsG0180002218.01 | 0.866345 | 3.051889e-65 | 1.025286e-61 |
| MsG0180002217.01 | MsG0180002397.01 | 0.800623 | 1.321490e-48 | 6.182092e-46 |
| MsG0180002217.01 | MsG0180002428.01 | 0.842701 | 2.201363e-58 | 3.365202e-55 |
| MsG0180002217.01 | MsG0180004120.01 | 0.831759 | 1.391523e-55 | 1.524681e-52 |
| MsG0180002217.01 | MsG0180004538.01 | 0.802641 | 5.093636e-49 | 2.508019e-46 |
| MsG0180002217.01 | MsG0180004583.01 | 0.831449 | 1.659016e-55 | 1.800996e-52 |
| MsG0180002217.01 | MsG0180005655.01 | 0.807762 | 4.313420e-50 | 2.422823e-47 |
| MsG0180002217.01 | MsG0280007919.01 | 0.840015 | 1.121069e-57 | 1.576314e-54 |
| MsG0180002217.01 | MsG0380012956.01 | 0.802889 | 4.528482e-49 | 2.243929e-46 |
| MsG0180002217.01 | MsG0380014666.01 | 0.842818 | 2.049425e-58 | 3.144322e-55 |
| MsG0180002217.01 | MsG0380015340.01 | 0.858628 | 7.228350e-63 | 1.856509e-59 |
| MsG0180002217.01 | MsG0380015394.01 | 0.812012 | 5.252328e-51 | 3.301178e-48 |
| MsG0180002217.01 | MsG0380015954.01 | 0.823085 | 1.674606e-53 | 1.427689e-50 |
| MsG0180002217.01 | MsG0380017128.01 | 0.821800 | 3.329315e-53 | 2.736455e-50 |
| MsG0180002217.01 | MsG0380017332.01 | 0.814959 | 1.180859e-51 | 8.035674e-49 |
| MsG0180002217.01 | MsG0380017605.01 | 0.816817 | 4.547179e-52 | 3.255659e-49 |
| MsG0180002217.01 | MsG0380017632.01 | 0.804329 | 2.276346e-49 | 1.170392e-46 |
| MsG0180002217.01 | MsG0380017696.01 | 0.812936 | 3.298997e-51 | 2.125173e-48 |
| MsG0180002217.01 | MsG0480018228.01 | 0.841599 | 4.309435e-58 | 6.364328e-55 |
| MsG0180002217.01 | MsG0480020398.01 | 0.840890 | 6.618568e-58 | 9.561430e-55 |
| MsG0180002217.01 | MsG0480020856.01 | 0.826070 | 3.318651e-54 | 3.079787e-51 |
| MsG0180002217.01 | MsG0480020921.01 | 0.818893 | 1.545354e-52 | 1.171465e-49 |
| MsG0180002217.01 | MsG0480020926.01 | 0.815616 | 8.439235e-52 | 5.847496e-49 |
| MsG0180002217.01 | MsG0480020970.01 | 0.803052 | 4.190402e-49 | 2.084927e-46 |
| MsG0180002217.01 | MsG0480021100.01 | 0.831345 | 1.759161e-55 | 1.903759e-52 |
| MsG0180002217.01 | MsG0480021301.01 | 0.811597 | 6.465242e-51 | 4.018646e-48 |
| MsG0180002217.01 | MsG0480021704.01 | 0.801626 | 8.240699e-49 | 3.953992e-46 |
| MsG0180002217.01 | MsG0480021773.01 | 0.816922 | 4.308256e-52 | 3.093468e-49 |
| MsG0180002217.01 | MsG0480021902.01 | 0.850142 | 2.055414e-60 | 3.987247e-57 |
| MsG0180002217.01 | MsG0480022307.01 | 0.855677 | 5.373182e-62 | 1.250466e-58 |
| MsG0180002217.01 | MsG0480022378.01 | 0.809899 | 1.506471e-50 | 8.953284e-48 |
| MsG0180002217.01 | MsG0480022991.01 | 0.810667 | 1.028719e-50 | 6.239122e-48 |
| MsG0180002217.01 | MsG0480023003.01 | 0.824866 | 6.398306e-54 | 5.738124e-51 |
| MsG0180002217.01 | MsG0480023426.01 | 0.833494 | 5.164940e-56 | 5.957977e-53 |
| MsG0180002217.01 | MsG0480023706.01 | 0.824885 | 6.334091e-54 | 5.683668e-51 |
| MsG0180002217.01 | MsG0480023842.01 | 0.808617 | 2.836771e-50 | 1.629468e-47 |
| MsG0180002217.01 | MsG0580024276.01 | 0.803027 | 4.240598e-49 | 2.108564e-46 |
| MsG0180002217.01 | MsG0580025177.01 | 0.807849 | 4.135070e-50 | 2.327861e-47 |
| MsG0180002217.01 | MsG0580025504.01 | 0.832674 | 8.262684e-56 | 9.300616e-53 |
| MsG0180002217.01 | MsG0580028955.01 | 0.845252 | 4.559312e-59 | 7.553107e-56 |
| MsG0180002217.01 | MsG0780036534.01 | 0.826022 | 3.407414e-54 | 3.157962e-51 |
| MsG0180002217.01 | MsG0780038736.01 | 0.841606 | 4.291463e-58 | 6.339398e-55 |
| MsG0180002217.01 | MsG0780040641.01 | 0.807110 | 5.931349e-50 | 3.275465e-47 |
| MsG0180002217.01 | MsG0780040899.01 | 0.822488 | 2.306110e-53 | 1.933013e-50 |
| MsG0180002217.01 | MsG0780041273.01 | 0.830125 | 3.502968e-55 | 3.657321e-52 |
| MsG0180002217.01 | MsG0780041359.01 | 0.806888 | 6.609698e-50 | 3.629244e-47 |
| MsG0180002217.01 | MsG0880044103.01 | 0.824396 | 8.257216e-54 | 7.304590e-51 |
| MsG0180002217.01 | MsG0880044437.01 | 0.831152 | 1.962710e-55 | 2.111846e-52 |
| MsG0180002217.01 | MsG0880045612.01 | 0.807579 | 4.716999e-50 | 2.636825e-47 |
| MsG0180002217.01 | MsG0880045647.01 | 0.829696 | 4.456283e-55 | 4.595700e-52 |
| MsG0180002217.01 | MsG0880047649.01 | 0.815951 | 7.105886e-52 | 4.968422e-49 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 26 sgRNAs with CRISPR-Local
Find 33 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCAGAATCGGTCTGGGTTT+GGG | 0.237047 | 1:-35028611 | MsG0180002217.01.T01:CDS |
| GGGGCAAGAAGGGGTTCTTC+TGG | 0.330658 | 1:-35028591 | MsG0180002217.01.T01:CDS |
| ATGCAGAATCGGTCTGGGTT+TGG | 0.338926 | 1:-35028612 | MsG0180002217.01.T01:CDS |
| TCTGGAAAAGCAGGCATGTT+TGG | 0.352490 | 1:-35028573 | MsG0180002217.01.T01:CDS |
| AAAAGCAGGCATGTTTGGTT+TGG | 0.377636 | 1:-35028568 | MsG0180002217.01.T01:CDS |
| GTCTGGGTTTGGGGCAAGAA+GGG | 0.415976 | 1:-35028601 | MsG0180002217.01.T01:CDS |
| GGGAACATGCAGAATCGGTC+TGG | 0.418807 | 1:-35028618 | MsG0180002217.01.T01:CDS |
| TCTGGGTTTGGGGCAAGAAG+GGG | 0.444554 | 1:-35028600 | MsG0180002217.01.T01:CDS |
| GCCGTCGGAGGCGGCGAAGA+AGG | 0.452615 | 1:-35028784 | MsG0180002217.01.T01:CDS |
| TGCGGTCCAGAATGAGGCTT+CGG | 0.467026 | 1:-35028679 | MsG0180002217.01.T01:CDS |
| GAGGACGATGCCGTCGGAGG+CGG | 0.474359 | 1:-35028793 | None:intergenic |
| GGAACATGCAGAATCGGTCT+GGG | 0.489835 | 1:-35028617 | MsG0180002217.01.T01:CDS |
| AGGGGTTCTTCTGGAAAAGC+AGG | 0.505225 | 1:-35028582 | MsG0180002217.01.T01:CDS |
| GCCTTCTTCGCCGCCTCCGA+CGG | 0.505987 | 1:+35028783 | MsG0180002218.01.T01:CDS |
| GGTCTGGGTTTGGGGCAAGA+AGG | 0.509820 | 1:-35028602 | MsG0180002217.01.T01:CDS |
| TTCTTCTGCGGTCCAGAATG+AGG | 0.510696 | 1:-35028685 | MsG0180002217.01.T01:CDS |
| GGATTGGGAACATGCAGAAT+CGG | 0.521690 | 1:-35028623 | MsG0180002217.01.T01:CDS |
| GCAGAATCGGTCTGGGTTTG+GGG | 0.524980 | 1:-35028610 | MsG0180002217.01.T01:CDS |
| CGAGGACATGTGAGATGAAG+TGG | 0.543318 | 1:-35028761 | MsG0180002217.01.T01:CDS |
| TTTCCTCCGATCTCAAGCAC+TGG | 0.566756 | 1:+35028715 | MsG0180002218.01.T01:CDS |
| TTCGAGGACGATGCCGTCGG+AGG | 0.583990 | 1:-35028796 | None:intergenic |
| CGGAGGCGGCGAAGAAGGCG+AGG | 0.590500 | 1:-35028779 | MsG0180002217.01.T01:CDS |
| TTGCCAGTGCTTGAGATCGG+AGG | 0.610798 | 1:-35028718 | MsG0180002217.01.T01:CDS |
| CACTGGCAAAACCTCACTGA+CGG | 0.643079 | 1:+35028732 | MsG0180002218.01.T01:CDS |
| AGTGGCGTTCGCCGTCAGTG+AGG | 0.654051 | 1:-35028743 | MsG0180002217.01.T01:CDS |
| GGAAAGATCGACTTCTTCTG+CGG | 0.673797 | 1:-35028697 | MsG0180002217.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AATCCAATACCCAAAAATCT+GGG | + | Chr1:35028708-35028727 | MsG0180002218.01.T01:CDS | 30.0% | |
| ! | TGTACATTTCCCAGATTTTT+GGG | - | Chr1:35028696-35028715 | MsG0180002217.01.T01:CDS | 30.0% |
| ! | TTGTACATTTCCCAGATTTT+TGG | - | Chr1:35028695-35028714 | MsG0180002217.01.T01:CDS | 30.0% |
| !!! | AGATTTTTGGGTATTGGATT+GGG | - | Chr1:35028708-35028727 | MsG0180002217.01.T01:CDS | 30.0% |
| CAATCCAATACCCAAAAATC+TGG | + | Chr1:35028709-35028728 | MsG0180002218.01.T01:CDS | 35.0% | |
| !!! | CAGATTTTTGGGTATTGGAT+TGG | - | Chr1:35028707-35028726 | MsG0180002217.01.T01:CDS | 35.0% |
| !!! | TTTCCCAGATTTTTGGGTAT+TGG | - | Chr1:35028702-35028721 | MsG0180002217.01.T01:CDS | 35.0% |
| AAAAGCAGGCATGTTTGGTT+TGG | - | Chr1:35028778-35028797 | MsG0180002217.01.T01:CDS | 40.0% | |
| AAAAAGCCGAAGCCTCATTC+TGG | + | Chr1:35028676-35028695 | MsG0180002218.01.T01:CDS | 45.0% | |
| GGATTGGGAACATGCAGAAT+CGG | - | Chr1:35028723-35028742 | MsG0180002217.01.T01:CDS | 45.0% | |
| TCTGGAAAAGCAGGCATGTT+TGG | - | Chr1:35028773-35028792 | MsG0180002217.01.T01:CDS | 45.0% | |
| ! | GGAAAGATCGACTTCTTCTG+CGG | - | Chr1:35028649-35028668 | MsG0180002217.01.T01:CDS | 45.0% |
| ! | GTTTTGCCAGTGCTTGAGAT+CGG | - | Chr1:35028625-35028644 | MsG0180002217.01.T01:CDS | 45.0% |
| ATGCAGAATCGGTCTGGGTT+TGG | - | Chr1:35028734-35028753 | MsG0180002217.01.T01:CDS | 50.0% | |
| CACTGGCAAAACCTCACTGA+CGG | + | Chr1:35028617-35028636 | None:intergenic | 50.0% | |
| CGAGGACATGTGAGATGAAG+TGG | - | Chr1:35028585-35028604 | MsG0180002217.01.T01:CDS | 50.0% | |
| GGAACATGCAGAATCGGTCT+GGG | - | Chr1:35028729-35028748 | MsG0180002217.01.T01:CDS | 50.0% | |
| TGCAGAATCGGTCTGGGTTT+GGG | - | Chr1:35028735-35028754 | MsG0180002217.01.T01:CDS | 50.0% | |
| TTCTTCTGCGGTCCAGAATG+AGG | - | Chr1:35028661-35028680 | MsG0180002217.01.T01:CDS | 50.0% | |
| TTTCCTCCGATCTCAAGCAC+TGG | + | Chr1:35028634-35028653 | MsG0180002218.01.T01:CDS | 50.0% | |
| ! | AGGGGTTCTTCTGGAAAAGC+AGG | - | Chr1:35028764-35028783 | MsG0180002217.01.T01:CDS | 50.0% |
| GCAGAATCGGTCTGGGTTTG+GGG | - | Chr1:35028736-35028755 | MsG0180002217.01.T01:CDS | 55.0% | |
| GGGAACATGCAGAATCGGTC+TGG | - | Chr1:35028728-35028747 | MsG0180002217.01.T01:CDS | 55.0% | |
| TGCGGTCCAGAATGAGGCTT+CGG | - | Chr1:35028667-35028686 | MsG0180002217.01.T01:CDS | 55.0% | |
| TTGCCAGTGCTTGAGATCGG+AGG | - | Chr1:35028628-35028647 | MsG0180002217.01.T01:CDS | 55.0% | |
| ! | GTCTGGGTTTGGGGCAAGAA+GGG | - | Chr1:35028745-35028764 | MsG0180002217.01.T01:CDS | 55.0% |
| ! | TCTGGGTTTGGGGCAAGAAG+GGG | - | Chr1:35028746-35028765 | MsG0180002217.01.T01:CDS | 55.0% |
| GGGGCAAGAAGGGGTTCTTC+TGG | - | Chr1:35028755-35028774 | MsG0180002217.01.T01:CDS | 60.0% | |
| ! | GGTCTGGGTTTGGGGCAAGA+AGG | - | Chr1:35028744-35028763 | MsG0180002217.01.T01:CDS | 60.0% |
| AGTGGCGTTCGCCGTCAGTG+AGG | - | Chr1:35028603-35028622 | MsG0180002217.01.T01:CDS | 65.0% | |
| GCCTTCTTCGCCGCCTCCGA+CGG | + | Chr1:35028566-35028585 | None:intergenic | 70.0% | |
| CGGAGGCGGCGAAGAAGGCG+AGG | - | Chr1:35028567-35028586 | MsG0180002217.01.T01:CDS | 75.0% | |
| GCCGTCGGAGGCGGCGAAGA+AGG | - | Chr1:35028562-35028581 | MsG0180002217.01.T01:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 35028560 | 35028808 | 35028560 | ID=MsG0180002217.01;Name=MsG0180002217.01 |
| Chr1 | mRNA | 35028560 | 35028808 | 35028560 | ID=MsG0180002217.01.T01;Parent=MsG0180002217.01;Name=MsG0180002217.01.T01;_AED=0.40;_eAED=0.40;_QI=0|-1|0|1|-1|1|1|0|82 |
| Chr1 | exon | 35028560 | 35028808 | 35028560 | ID=MsG0180002217.01.T01:exon:34130;Parent=MsG0180002217.01.T01 |
| Chr1 | CDS | 35028560 | 35028808 | 35028560 | ID=MsG0180002217.01.T01:cds;Parent=MsG0180002217.01.T01 |
| Gene Sequence |
| Protein sequence |
