AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480021704.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 38 sgRNAs with CRISPR-Local

Find 84 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GACAGACTGGTTCCATTATT+TGG 0.093724 4:-63232365 None:intergenic
TGAATGTCGTTAACAGTTTC+AGG 0.178272 4:-63231792 None:intergenic
TTTGCTGTATCTGCAGAAAA+TGG 0.256970 4:+63231732 MsG0480021704.01.T01:CDS
GTGATTTAATAGTTGACTTC+TGG 0.304333 4:+63232566 MsG0480021704.01.T01:three_prime_UTR
TCAAATGAAGTCCCAAATAA+TGG 0.319473 4:+63232353 MsG0480021704.01.T01:CDS
ACAGACTGGTTCCATTATTT+GGG 0.352932 4:-63232364 None:intergenic
AGATACAGCAAAATCAATAT+AGG 0.363029 4:-63231721 None:intergenic
TGCTGTTATCTCAGCACTGC+TGG 0.389855 4:-63232387 None:intergenic
TTGCTGTATCTGCAGAAAAT+GGG 0.395630 4:+63231733 MsG0480021704.01.T01:CDS
AGATTACCTTGTTCACCATC+TGG 0.397013 4:-63231181 None:intergenic
TACATTGCAATCTTGAAGAT+TGG 0.397369 4:+63232619 MsG0480021704.01.T01:three_prime_UTR
GATACAGCAAAATCAATATA+GGG 0.403559 4:-63231720 None:intergenic
ATAGTTGACTTCTGGTGGTC+TGG 0.407653 4:+63232574 MsG0480021704.01.T01:three_prime_UTR
AAGACTGTGGTATGCTGGCT+CGG 0.435771 4:-63232466 None:intergenic
TCAACCTATTTGCTTCCATA+CGG 0.452181 4:-63232421 None:intergenic
GAGCCCGTATGGAAGCAAAT+AGG 0.454008 4:+63232417 MsG0480021704.01.T01:CDS
CTCAGCACTGCTGGACAGAC+TGG 0.455206 4:-63232378 None:intergenic
CTAGGAAGACTGTGGTATGC+TGG 0.471259 4:-63232471 None:intergenic
CAACCTATTTGCTTCCATAC+GGG 0.491149 4:-63232420 None:intergenic
AGCAGAGCAGAGAGCCCGTA+TGG 0.494494 4:+63232406 MsG0480021704.01.T01:CDS
GGGATGCTGTAGTCCACAAC+TGG 0.510182 4:-63231160 None:intergenic
GATTACCTTGTTCACCATCT+GGG 0.514063 4:-63231180 None:intergenic
ATTTAATAGTTGACTTCTGG+TGG 0.518733 4:+63232569 MsG0480021704.01.T01:three_prime_UTR
AAAGAAAGAGTTGCAATGGT+GGG 0.531989 4:+63231113 None:intergenic
TATGGAAGCAAATAGGTTGA+AGG 0.541904 4:+63232424 MsG0480021704.01.T01:CDS
ATAGGTTGAAGGCACTAGAA+AGG 0.545276 4:+63232435 MsG0480021704.01.T01:CDS
TTACAATAAATATCAAAGCT+AGG 0.548001 4:-63232489 None:intergenic
GAAGTCAACTATTAAATCAC+GGG 0.559043 4:-63232563 None:intergenic
TGCTGTATCTGCAGAAAATG+GGG 0.567337 4:+63231734 MsG0480021704.01.T01:CDS
GGTGGTTCACGCAACTTCGA+TGG 0.570994 4:-63231139 None:intergenic
AGACTGTGGTATGCTGGCTC+GGG 0.572569 4:-63232465 None:intergenic
AGCATCCCAGATGGTGAACA+AGG 0.583542 4:+63231175 MsG0480021704.01.T01:CDS
AGAAGTCAACTATTAAATCA+CGG 0.584982 4:-63232564 None:intergenic
GTTGCGTGAACCACCAGTTG+TGG 0.628493 4:+63231147 MsG0480021704.01.T01:CDS
AAGAAAGAGTTGCAATGGTG+GGG 0.638912 4:+63231114 None:intergenic
ATGCTGTAGTCCACAACTGG+TGG 0.647441 4:-63231157 None:intergenic
GTGGACTACAGCATCCCAGA+TGG 0.666651 4:+63231166 MsG0480021704.01.T01:CDS
TATCAAAGCTAGGAAGACTG+TGG 0.691472 4:-63232479 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAAAATCTAATTAGTTCTTT+TGG - Chr4:63231670-63231689 None:intergenic 15.0%
!! AATAATTACAAGAAATCTAG+AGG - Chr4:63232142-63232161 None:intergenic 20.0%
!! ATTCACATACAATTGAAAAA+AGG - Chr4:63231456-63231475 None:intergenic 20.0%
!! TTACAATAAATATCAAAGCT+AGG - Chr4:63232492-63232511 None:intergenic 20.0%
! AGAAGTCAACTATTAAATCA+CGG - Chr4:63232567-63232586 None:intergenic 25.0%
! AGATACAGCAAAATCAATAT+AGG - Chr4:63231724-63231743 None:intergenic 25.0%
! ATGATCAAATTGTCAATTGT+TGG - Chr4:63231510-63231529 None:intergenic 25.0%
! CAAAGAAATTAAACTCACTT+CGG - Chr4:63231853-63231872 None:intergenic 25.0%
! GATACAGCAAAATCAATATA+GGG - Chr4:63231723-63231742 None:intergenic 25.0%
! TATCATTAATAACACACTTC+TGG - Chr4:63232027-63232046 None:intergenic 25.0%
! TCACTAAATAGAAGTGATAA+TGG - Chr4:63232194-63232213 None:intergenic 25.0%
! TTGAAAAAAGGAAAATAACG+AGG - Chr4:63231444-63231463 None:intergenic 25.0%
!! AACTGATTCATATTTCAAAC+AGG + Chr4:63231594-63231613 MsG0480021704.01.T01:intron 25.0%
!! ACTGATTCATATTTCAAACA+GGG + Chr4:63231595-63231614 MsG0480021704.01.T01:intron 25.0%
!! AGTGTGTTATTAATGATACA+AGG + Chr4:63232029-63232048 MsG0480021704.01.T01:intron 25.0%
!! ATGAACTTTTCTTTTGTGAA+AGG + Chr4:63231923-63231942 MsG0480021704.01.T01:intron 25.0%
!! GAAATCTGTTAAAACACTTT+TGG + Chr4:63231945-63231964 MsG0480021704.01.T01:intron 25.0%
!! GTGTGTTATTAATGATACAA+GGG + Chr4:63232030-63232049 MsG0480021704.01.T01:intron 25.0%
!!! ATGAAGGAATACATTGATTT+TGG + Chr4:63231562-63231581 MsG0480021704.01.T01:intron 25.0%
!!! ATTTCTGAAACACTTATAAC+AGG - Chr4:63231403-63231422 None:intergenic 25.0%
AAAAAAGGAAAATAACGAGG+AGG - Chr4:63231441-63231460 None:intergenic 30.0%
AGACTGTTGTATTCTTTCAT+TGG + Chr4:63232070-63232089 MsG0480021704.01.T01:intron 30.0%
ATCTGATCCTAAGTTTGTAA+CGG + Chr4:63232235-63232254 MsG0480021704.01.T01:intron 30.0%
CAATATTCCAGTGAACTATA+TGG - Chr4:63231634-63231653 None:intergenic 30.0%
CAGGTGATTAATTCAAGAAA+TGG - Chr4:63231384-63231403 None:intergenic 30.0%
GAAGTCAACTATTAAATCAC+GGG - Chr4:63232566-63232585 None:intergenic 30.0%
GATAAACCAAATCGTAAACA+AGG + Chr4:63232212-63232231 MsG0480021704.01.T01:intron 30.0%
TACATTGCAATCTTGAAGAT+TGG + Chr4:63232619-63232638 MsG0480021704.01.T01:three_prime_UTR 30.0%
TCAAATGAAGTCCCAAATAA+TGG + Chr4:63232353-63232372 MsG0480021704.01.T01:CDS 30.0%
TGTGAGTAATCTGAATTGTT+TGG + Chr4:63231993-63232012 MsG0480021704.01.T01:intron 30.0%
TTACTTGCGTCTGAATTAAT+AGG + Chr4:63231253-63231272 MsG0480021704.01.T01:intron 30.0%
TTCTAGACGAACTTTACTAT+AGG - Chr4:63231322-63231341 None:intergenic 30.0%
! ATTACCTTCTCTTTTATTGC+AGG + Chr4:63232259-63232278 MsG0480021704.01.T01:intron 30.0%
! ATTTAATAGTTGACTTCTGG+TGG + Chr4:63232569-63232588 MsG0480021704.01.T01:three_prime_UTR 30.0%
! GTGATTTAATAGTTGACTTC+TGG + Chr4:63232566-63232585 MsG0480021704.01.T01:three_prime_UTR 30.0%
!!! TTTTCAGTTGTGCTACTTTT+GGG - Chr4:63232329-63232348 None:intergenic 30.0%
ACCATAGGTAGACAAAATGA+AGG + Chr4:63231546-63231565 MsG0480021704.01.T01:intron 35.0%
ATTTGATCATTGAAGCTCAC+TGG + Chr4:63231520-63231539 MsG0480021704.01.T01:intron 35.0%
GTGAACTATATGGAATCAGT+TGG - Chr4:63231624-63231643 None:intergenic 35.0%
TCAACCTATTTGCTTCCATA+CGG - Chr4:63232424-63232443 None:intergenic 35.0%
TGAATGTCGTTAACAGTTTC+AGG - Chr4:63231795-63231814 None:intergenic 35.0%
TTGCTGTATCTGCAGAAAAT+GGG + Chr4:63231733-63231752 MsG0480021704.01.T01:CDS 35.0%
! ACAGACTGGTTCCATTATTT+GGG - Chr4:63232367-63232386 None:intergenic 35.0%
! ACTGATTCCATATAGTTCAC+TGG + Chr4:63231624-63231643 MsG0480021704.01.T01:intron 35.0%
! AGGTATTTTGCTTAGATCTG+TGG + Chr4:63231273-63231292 MsG0480021704.01.T01:intron 35.0%
! ATTGTTGGTTGTAATCTCTC+TGG - Chr4:63231495-63231514 None:intergenic 35.0%
! TATGGAAGCAAATAGGTTGA+AGG + Chr4:63232424-63232443 MsG0480021704.01.T01:CDS 35.0%
! TCAGATCCTTGTTTACGATT+TGG - Chr4:63232221-63232240 None:intergenic 35.0%
! TCATTTTGTCTACCTATGGT+TGG - Chr4:63231546-63231565 None:intergenic 35.0%
! TCCTTCATTTTGTCTACCTA+TGG - Chr4:63231550-63231569 None:intergenic 35.0%
! TTTGCTGTATCTGCAGAAAA+TGG + Chr4:63231732-63231751 MsG0480021704.01.T01:CDS 35.0%
!! ACATGCTGACTTCATAACAA+AGG - Chr4:63232165-63232184 None:intergenic 35.0%
!!! CTTTTCAGTTGTGCTACTTT+TGG - Chr4:63232330-63232349 None:intergenic 35.0%
AGATTACCTTGTTCACCATC+TGG - Chr4:63231184-63231203 None:intergenic 40.0%
CAACCTATTTGCTTCCATAC+GGG - Chr4:63232423-63232442 None:intergenic 40.0%
GATTACCTTGTTCACCATCT+GGG - Chr4:63231183-63231202 None:intergenic 40.0%
GGTAATGCCGTTACAAACTT+AGG - Chr4:63232245-63232264 None:intergenic 40.0%
GGTTCCTGCAATAAAAGAGA+AGG - Chr4:63232266-63232285 None:intergenic 40.0%
GTATTTGCACTTCGCTATCA+AGG + Chr4:63231212-63231231 MsG0480021704.01.T01:intron 40.0%
TATCAAAGCTAGGAAGACTG+TGG - Chr4:63232482-63232501 None:intergenic 40.0%
TGCTGTATCTGCAGAAAATG+GGG + Chr4:63231734-63231753 MsG0480021704.01.T01:CDS 40.0%
TTGGAGACTATTTGAACGTC+TGG + Chr4:63232089-63232108 MsG0480021704.01.T01:intron 40.0%
! CGCAGAAATCACATTTTGCA+CGG - Chr4:63232297-63232316 None:intergenic 40.0%
! GACAGACTGGTTCCATTATT+TGG - Chr4:63232368-63232387 None:intergenic 40.0%
! GCAGAAATCACATTTTGCAC+GGG - Chr4:63232296-63232315 None:intergenic 40.0%
!! ATAGGTTGAAGGCACTAGAA+AGG + Chr4:63232435-63232454 MsG0480021704.01.T01:CDS 40.0%
! ATAGTTGACTTCTGGTGGTC+TGG + Chr4:63232574-63232593 MsG0480021704.01.T01:three_prime_UTR 45.0%
!! AAAATAACGAGGAGGCGTTC+TGG - Chr4:63231433-63231452 None:intergenic 45.0%
!! AAATAACGAGGAGGCGTTCT+GGG - Chr4:63231432-63231451 None:intergenic 45.0%
AGCATCCCAGATGGTGAACA+AGG + Chr4:63231175-63231194 MsG0480021704.01.T01:CDS 50.0%
ATGCTGTAGTCCACAACTGG+TGG - Chr4:63231160-63231179 None:intergenic 50.0%
CTAGGAAGACTGTGGTATGC+TGG - Chr4:63232474-63232493 None:intergenic 50.0%
GAGCCCGTATGGAAGCAAAT+AGG + Chr4:63232417-63232436 MsG0480021704.01.T01:CDS 50.0%
TGCTGTTATCTCAGCACTGC+TGG - Chr4:63232390-63232409 None:intergenic 50.0%
! AAGACTGTGGTATGCTGGCT+CGG - Chr4:63232469-63232488 None:intergenic 50.0%
!! ACATTTTGCACGGGCTGAGA+AGG - Chr4:63232287-63232306 None:intergenic 50.0%
GAAGCTCACTGGCCAACCAT+AGG + Chr4:63231531-63231550 MsG0480021704.01.T01:intron 55.0%
GGGATGCTGTAGTCCACAAC+TGG - Chr4:63231163-63231182 None:intergenic 55.0%
GGTGGTTCACGCAACTTCGA+TGG - Chr4:63231142-63231161 None:intergenic 55.0%
GTTGCGTGAACCACCAGTTG+TGG + Chr4:63231147-63231166 MsG0480021704.01.T01:CDS 55.0%
! GTGGACTACAGCATCCCAGA+TGG + Chr4:63231166-63231185 MsG0480021704.01.T01:CDS 55.0%
!! AGACTGTGGTATGCTGGCTC+GGG - Chr4:63232468-63232487 None:intergenic 55.0%
AGCAGAGCAGAGAGCCCGTA+TGG + Chr4:63232406-63232425 MsG0480021704.01.T01:CDS 60.0%
CTCAGCACTGCTGGACAGAC+TGG - Chr4:63232381-63232400 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr4 gene 63231128 63232648 63231128 ID=MsG0480021704.01;Name=MsG0480021704.01
Chr4 mRNA 63231128 63232648 63231128 ID=MsG0480021704.01.T01;Parent=MsG0480021704.01;Name=MsG0480021704.01.T01;_AED=0.42;_eAED=0.44;_QI=0|0.75|0.8|1|0.25|0.2|5|155|150
Chr4 exon 63232281 63232648 63232281 ID=MsG0480021704.01.T01:exon:20874;Parent=MsG0480021704.01.T01
Chr4 exon 63231684 63231854 63231684 ID=MsG0480021704.01.T01:exon:20873;Parent=MsG0480021704.01.T01
Chr4 exon 63231128 63231196 63231128 ID=MsG0480021704.01.T01:exon:20872;Parent=MsG0480021704.01.T01
Chr4 CDS 63231128 63231196 63231128 ID=MsG0480021704.01.T01:cds;Parent=MsG0480021704.01.T01
Chr4 CDS 63231684 63231854 63231684 ID=MsG0480021704.01.T01:cds;Parent=MsG0480021704.01.T01
Chr4 CDS 63232281 63232493 63232281 ID=MsG0480021704.01.T01:cds;Parent=MsG0480021704.01.T01
Chr4 three_prime_UTR 63232494 63232648 63232494 ID=MsG0480021704.01.T01:three_prime_utr;Parent=MsG0480021704.01.T01
Gene Sequence

>MsG0480021704.01.T01

ATGGTGGGGATCCATCGAAGTTGCGTGAACCACCAGTTGTGGACTACAGCATCCCAGATGGTGAACAAGATTTTATTTATTTTATTAAGTATCTTTGCTAAGTATCCCTATATTGATTTTGCTGTATCTGCAGAAAATGGGGAAGCAAGTCATCAAGAGAATGAGATGTTTGCAGAACCTGAAACTGTTAACGACATTCAAGAAGATTTGTTCAATGATATATATGATAAAGCTACCGAAGAACCTTCTCAGCCCGTGCAAAATGTGATTTCTGCGAGTACTGATCCCAAAAGTAGCACAACTGAAAAGACATCAAATGAAGTCCCAAATAATGGAACCAGTCTGTCCAGCAGTGCTGAGATAACAGCAGAGCAGAGAGCCCGTATGGAAGCAAATAGGTTGAAGGCACTAGAAAGGCGTGCAGCCCGAGCCAGCATACCACAGTCTTCCTAG

Protein sequence

>MsG0480021704.01.T01

MVGIHRSCVNHQLWTTASQMVNKILFILLSIFAKYPYIDFAVSAENGEASHQENEMFAEPETVNDIQEDLFNDIYDKATEEPSQPVQNVISASTDPKSSTTEKTSNEVPNNGTSLSSSAEITAEQRARMEANRLKALERRAARASIPQSS*