Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180002428.01.T01 | XP_013467371.1 | 100 | 97 | 0 | 0 | 1 | 97 | 19 | 115 | 9.57E-63 | 203 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180002428.01.T01 | P49730 | 89.691 | 97 | 10 | 0 | 1 | 97 | 19 | 115 | 2.85E-59 | 187 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180002428.01.T01 | A0A072VJ25 | 100.000 | 97 | 0 | 0 | 1 | 97 | 19 | 115 | 4.57e-63 | 203 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048797.01 | MsG0180002428.01 | 0.820137 | 8.039913e-53 | 6.309360e-50 |
| MsG0180001293.01 | MsG0180002428.01 | 0.809948 | 1.470119e-50 | 8.748702e-48 |
| MsG0180001719.01 | MsG0180002428.01 | 0.855048 | 8.189824e-62 | 1.866391e-58 |
| MsG0180002134.01 | MsG0180002428.01 | 0.811441 | 6.990584e-51 | 4.327748e-48 |
| MsG0180002135.01 | MsG0180002428.01 | 0.830143 | 3.466917e-55 | 3.621746e-52 |
| MsG0180002217.01 | MsG0180002428.01 | 0.842701 | 2.201363e-58 | 3.365202e-55 |
| MsG0180002218.01 | MsG0180002428.01 | 0.825061 | 5.754854e-54 | 5.189605e-51 |
| MsG0180002397.01 | MsG0180002428.01 | 0.814018 | 1.907377e-51 | 1.265369e-48 |
| MsG0180002428.01 | MsG0180003857.01 | 0.818119 | 2.314865e-52 | 1.717575e-49 |
| MsG0180002428.01 | MsG0180003905.01 | 0.805023 | 1.630724e-49 | 8.535680e-47 |
| MsG0180002428.01 | MsG0180004120.01 | 0.845235 | 4.609894e-59 | 7.632235e-56 |
| MsG0180002428.01 | MsG0180004185.01 | 0.813341 | 2.687781e-51 | 1.750640e-48 |
| MsG0180002428.01 | MsG0180004299.01 | 0.805951 | 1.041658e-49 | 5.584121e-47 |
| MsG0180002428.01 | MsG0180004538.01 | 0.819635 | 1.047388e-52 | 8.104197e-50 |
| MsG0180002428.01 | MsG0180004583.01 | 0.849447 | 3.215225e-60 | 6.096624e-57 |
| MsG0180002428.01 | MsG0180005655.01 | 0.843582 | 1.282697e-58 | 2.016144e-55 |
| MsG0180002428.01 | MsG0280006398.01 | 0.806955 | 6.398247e-50 | 3.519210e-47 |
| MsG0180002428.01 | MsG0280007695.01 | 0.807294 | 5.422677e-50 | 3.009113e-47 |
| MsG0180002428.01 | MsG0280007919.01 | 0.826052 | 3.351641e-54 | 3.108917e-51 |
| MsG0180002428.01 | MsG0280009216.01 | 0.802040 | 6.775233e-49 | 3.285283e-46 |
| MsG0180002428.01 | MsG0380014542.01 | 0.836188 | 1.083347e-56 | 1.355222e-53 |
| MsG0180002428.01 | MsG0380014544.01 | 0.834567 | 2.781586e-56 | 3.313966e-53 |
| MsG0180002428.01 | MsG0380014666.01 | 0.852307 | 5.030943e-61 | 1.046943e-57 |
| MsG0180002428.01 | MsG0380014807.01 | 0.810031 | 1.411017e-50 | 8.415552e-48 |
| MsG0180002428.01 | MsG0380015219.01 | 0.807371 | 5.222390e-50 | 2.903685e-47 |
| MsG0180002428.01 | MsG0380015340.01 | 0.845942 | 2.964846e-59 | 5.022160e-56 |
| MsG0180002428.01 | MsG0380015954.01 | 0.809184 | 2.145097e-50 | 1.250980e-47 |
| MsG0180002428.01 | MsG0380016165.01 | 0.802495 | 5.459757e-49 | 2.678287e-46 |
| MsG0180002428.01 | MsG0380017128.01 | 0.807044 | 6.126188e-50 | 3.377401e-47 |
| MsG0180002428.01 | MsG0380017332.01 | 0.836469 | 9.186975e-57 | 1.158881e-53 |
| MsG0180002428.01 | MsG0380017605.01 | 0.838967 | 2.099618e-57 | 2.857774e-54 |
| MsG0180002428.01 | MsG0380017696.01 | 0.830749 | 2.464741e-55 | 2.620649e-52 |
| MsG0180002428.01 | MsG0380017932.01 | 0.810714 | 1.004961e-50 | 6.102641e-48 |
| MsG0180002428.01 | MsG0480018228.01 | 0.879600 | 1.091944e-69 | 5.956392e-66 |
| MsG0180002428.01 | MsG0480020398.01 | 0.885753 | 6.195380e-72 | 4.305301e-68 |
| MsG0180002428.01 | MsG0480020937.01 | 0.827201 | 1.782672e-54 | 1.709269e-51 |
| MsG0180002428.01 | MsG0480020970.01 | 0.810755 | 9.846658e-51 | 5.986063e-48 |
| MsG0180002428.01 | MsG0480021100.01 | 0.849881 | 2.431875e-60 | 4.677640e-57 |
| MsG0180002428.01 | MsG0480021301.01 | 0.824331 | 8.553286e-54 | 7.552551e-51 |
| MsG0180002428.01 | MsG0480021773.01 | 0.852709 | 3.864700e-61 | 8.145950e-58 |
| MsG0180002428.01 | MsG0480021902.01 | 0.861327 | 1.109357e-63 | 3.127501e-60 |
| MsG0180002428.01 | MsG0480022307.01 | 0.871778 | 5.273585e-67 | 2.153722e-63 |
| MsG0180002428.01 | MsG0480022378.01 | 0.811962 | 5.383611e-51 | 3.379128e-48 |
| MsG0180002428.01 | MsG0480022784.01 | 0.827231 | 1.753778e-54 | 1.682918e-51 |
| MsG0180002428.01 | MsG0480023003.01 | 0.823763 | 1.162324e-53 | 1.010172e-50 |
| MsG0180002428.01 | MsG0480023414.01 | 0.807268 | 5.491431e-50 | 3.045232e-47 |
| MsG0180002428.01 | MsG0480023424.01 | 0.805298 | 1.428090e-49 | 7.528253e-47 |
| MsG0180002428.01 | MsG0480023426.01 | 0.859234 | 4.761662e-63 | 1.248750e-59 |
| MsG0180002428.01 | MsG0480023706.01 | 0.853013 | 3.164064e-61 | 6.738887e-58 |
| MsG0180002428.01 | MsG0480023842.01 | 0.857893 | 1.196745e-62 | 2.999130e-59 |
| MsG0180002428.01 | MsG0480023913.01 | 0.817112 | 3.904770e-52 | 2.818338e-49 |
| MsG0180002428.01 | MsG0480024019.01 | 0.826045 | 3.364777e-54 | 3.120399e-51 |
| MsG0180002428.01 | MsG0580024276.01 | 0.834195 | 3.448169e-56 | 4.061937e-53 |
| MsG0180002428.01 | MsG0580024787.01 | 0.808163 | 3.545203e-50 | 2.012534e-47 |
| MsG0180002428.01 | MsG0580025504.01 | 0.844841 | 5.887243e-59 | 9.626167e-56 |
| MsG0180002428.01 | MsG0580026343.01 | 0.811081 | 8.368693e-51 | 5.131745e-48 |
| MsG0180002428.01 | MsG0580028927.01 | 0.812435 | 4.246424e-51 | 2.699910e-48 |
| MsG0180002428.01 | MsG0580028955.01 | 0.851696 | 7.501747e-61 | 1.531017e-57 |
| MsG0180002428.01 | MsG0680035350.01 | 0.800109 | 1.681812e-48 | 7.765655e-46 |
| MsG0180002428.01 | MsG0680035776.01 | 0.829987 | 3.784097e-55 | 3.935324e-52 |
| MsG0180002428.01 | MsG0780036534.01 | 0.804094 | 2.547211e-49 | 1.301836e-46 |
| MsG0180002428.01 | MsG0780038647.01 | 0.819933 | 8.951978e-53 | 6.985001e-50 |
| MsG0180002428.01 | MsG0780038736.01 | 0.840221 | 9.907042e-58 | 1.401789e-54 |
| MsG0180002428.01 | MsG0780040459.01 | 0.809587 | 1.757802e-50 | 1.036209e-47 |
| MsG0180002428.01 | MsG0780040554.01 | 0.809757 | 1.615603e-50 | 9.567022e-48 |
| MsG0180002428.01 | MsG0780040641.01 | 0.827515 | 1.499360e-54 | 1.450691e-51 |
| MsG0180002428.01 | MsG0780040899.01 | 0.856443 | 3.206790e-62 | 7.655687e-59 |
| MsG0180002428.01 | MsG0780041219.01 | 0.808025 | 3.793670e-50 | 2.145628e-47 |
| MsG0180002428.01 | MsG0780041232.01 | 0.800632 | 1.316015e-48 | 6.157954e-46 |
| MsG0180002428.01 | MsG0780041273.01 | 0.818921 | 1.523167e-52 | 1.155573e-49 |
| MsG0180002428.01 | MsG0780041359.01 | 0.839006 | 2.050536e-57 | 2.794499e-54 |
| MsG0180002428.01 | MsG0880043684.01 | 0.806244 | 9.039550e-50 | 4.882396e-47 |
| MsG0180002428.01 | MsG0880043714.01 | 0.802063 | 6.702524e-49 | 3.251980e-46 |
| MsG0180002428.01 | MsG0880044103.01 | 0.800567 | 1.356731e-48 | 6.337920e-46 |
| MsG0180002428.01 | MsG0880044437.01 | 0.838975 | 2.088819e-57 | 2.843952e-54 |
| MsG0180002428.01 | MsG0880045356.01 | 0.834857 | 2.351618e-56 | 2.825932e-53 |
| MsG0180002428.01 | MsG0880045439.01 | 0.803497 | 3.389489e-49 | 1.705911e-46 |
| MsG0180002428.01 | MsG0880045612.01 | 0.810727 | 9.984289e-51 | 6.065106e-48 |
| MsG0180002428.01 | MsG0880045647.01 | 0.862014 | 6.840088e-64 | 1.973433e-60 |
| MsG0180002428.01 | MsG0880045879.01 | 0.818352 | 2.049544e-52 | 1.530658e-49 |
| MsG0180002428.01 | MsG0880046904.01 | 0.853690 | 2.023229e-61 | 4.408343e-58 |
| MsG0180002428.01 | MsG0880046905.01 | 0.804128 | 2.506874e-49 | 1.282281e-46 |
| MsG0180002428.01 | MsG0880047414.01 | 0.806287 | 8.851176e-50 | 4.785766e-47 |
| MsG0180002428.01 | MsG0880047649.01 | 0.825617 | 4.250594e-54 | 3.894262e-51 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180002428.01.T01 | MTR_1g050472 | 100.000 | 97 | 0 | 0 | 1 | 97 | 19 | 115 | 1.16e-66 | 203 |
| MsG0180002428.01.T01 | MTR_0268s0080 | 96.907 | 97 | 3 | 0 | 1 | 97 | 19 | 115 | 1.52e-65 | 201 |
| MsG0180002428.01.T01 | MTR_7g029110 | 96.907 | 97 | 3 | 0 | 1 | 97 | 19 | 115 | 2.32e-65 | 200 |
| MsG0180002428.01.T01 | MTR_8g469010 | 94.845 | 97 | 5 | 0 | 1 | 97 | 19 | 115 | 1.21e-64 | 198 |
| MsG0180002428.01.T01 | MTR_4g117370 | 78.351 | 97 | 21 | 0 | 1 | 97 | 31 | 127 | 1.78e-53 | 170 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180002428.01.T01 | AT3G27060 | 85.714 | 98 | 13 | 1 | 1 | 97 | 19 | 116 | 1.83e-55 | 175 |
| MsG0180002428.01.T01 | AT3G23580 | 80.412 | 97 | 19 | 0 | 1 | 97 | 31 | 127 | 4.50e-54 | 171 |
Find 23 sgRNAs with CRISPR-Local
Find 34 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGTGGCGTTCGCCGTCTGTT+AGG | 0.311051 | 1:-38330861 | None:intergenic |
| GAATCGCTTTATACGTTTCC+AGG | 0.331867 | 1:-38331045 | None:intergenic |
| TCCGAAGCACGTGCCTTCTA+TGG | 0.349395 | 1:+38330973 | MsG0180002428.01.T01:CDS |
| ACCATAGAAGGCACGTGCTT+CGG | 0.385949 | 1:-38330974 | None:intergenic |
| GATTTCTTTCATGAATCTAC+CGG | 0.435670 | 1:-38330944 | None:intergenic |
| AGGCACGTGCTTCGGAGACT+TGG | 0.444676 | 1:-38330966 | None:intergenic |
| GCCGTCGGAGGCGGCGAAGA+AGG | 0.452615 | 1:-38330902 | None:intergenic |
| TGCAATCTGGAAACCATAGA+AGG | 0.453514 | 1:-38330986 | None:intergenic |
| GAGGACGATGCCGTCGGAGG+CGG | 0.474359 | 1:-38330911 | None:intergenic |
| GGCGGTCCAGAATGAGGCTT+CGG | 0.476778 | 1:-38330797 | None:intergenic |
| GAGATGTATTCTCTCTCTCC+TGG | 0.479017 | 1:+38331027 | MsG0180002428.01.T01:CDS |
| GGAGGAAAGATCGACTTCTT+CGG | 0.499480 | 1:-38330818 | None:intergenic |
| GCCTTCTTCGCCGCCTCCGA+CGG | 0.505987 | 1:+38330901 | MsG0180002428.01.T01:CDS |
| TTCTTCGGCGGTCCAGAATG+AGG | 0.524296 | 1:-38330803 | None:intergenic |
| TTTCCTCCGATCTCAAGCAC+TGG | 0.566756 | 1:+38330833 | MsG0180002428.01.T01:CDS |
| TTCGAGGACGATGCCGTCGG+AGG | 0.583990 | 1:-38330914 | None:intergenic |
| CGAGGACGTGAGAGATGAAG+TGG | 0.584744 | 1:-38330879 | None:intergenic |
| CGGAGGCGGCGAAGAAGGCG+AGG | 0.590500 | 1:-38330897 | None:intergenic |
| CACTGGCAAAACCTAACAGA+CGG | 0.606745 | 1:+38330850 | MsG0180002428.01.T01:CDS |
| TTGCCAGTGCTTGAGATCGG+AGG | 0.610798 | 1:-38330836 | None:intergenic |
| ATCGTCCTCGAAAATCTCGC+CGG | 0.639774 | 1:+38330925 | MsG0180002428.01.T01:CDS |
| GGAAAGATCGACTTCTTCGG+CGG | 0.687608 | 1:-38330815 | None:intergenic |
| AGAGAGAATACATCTCAGAG+TGG | 0.694344 | 1:-38331020 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AATCCAATACCCAAAAATCT+GGG | + | Chr1:38330759-38330778 | MsG0180002428.01.T01:CDS | 30.0% | |
| GATTTCTTTCATGAATCTAC+CGG | - | Chr1:38330947-38330966 | None:intergenic | 30.0% | |
| ! | TGTACATTTCCCAGATTTTT+GGG | - | Chr1:38330771-38330790 | None:intergenic | 30.0% |
| ! | TTGTACATTTCCCAGATTTT+TGG | - | Chr1:38330772-38330791 | None:intergenic | 30.0% |
| !!! | AGATTTTTGGGTATTGGATT+GGG | - | Chr1:38330759-38330778 | None:intergenic | 30.0% |
| CAATCCAATACCCAAAAATC+TGG | + | Chr1:38330758-38330777 | MsG0180002428.01.T01:CDS | 35.0% | |
| ! | GGATGTTTTCAATTGCAATC+TGG | - | Chr1:38331002-38331021 | None:intergenic | 35.0% |
| !!! | CAGATTTTTGGGTATTGGAT+TGG | - | Chr1:38330760-38330779 | None:intergenic | 35.0% |
| !!! | TTTCCCAGATTTTTGGGTAT+TGG | - | Chr1:38330765-38330784 | None:intergenic | 35.0% |
| AGAGAGAATACATCTCAGAG+TGG | - | Chr1:38331023-38331042 | None:intergenic | 40.0% | |
| TGCAATCTGGAAACCATAGA+AGG | - | Chr1:38330989-38331008 | None:intergenic | 40.0% | |
| AAAAAGCCGAAGCCTCATTC+TGG | + | Chr1:38330791-38330810 | MsG0180002428.01.T01:CDS | 45.0% | |
| CACTGGCAAAACCTAACAGA+CGG | + | Chr1:38330850-38330869 | MsG0180002428.01.T01:CDS | 45.0% | |
| GAGATGTATTCTCTCTCTCC+TGG | + | Chr1:38331027-38331046 | MsG0180002428.01.T01:CDS | 45.0% | |
| ! | GGAGGAAAGATCGACTTCTT+CGG | - | Chr1:38330821-38330840 | None:intergenic | 45.0% |
| ! | GTTTTGCCAGTGCTTGAGAT+CGG | - | Chr1:38330842-38330861 | None:intergenic | 45.0% |
| ATCGTCCTCGAAAATCTCGC+CGG | + | Chr1:38330925-38330944 | MsG0180002428.01.T01:CDS | 50.0% | |
| TTTCCTCCGATCTCAAGCAC+TGG | + | Chr1:38330833-38330852 | MsG0180002428.01.T01:CDS | 50.0% | |
| ! | ACCATAGAAGGCACGTGCTT+CGG | - | Chr1:38330977-38330996 | None:intergenic | 50.0% |
| ! | ATCTACCGGCGAGATTTTCG+AGG | - | Chr1:38330933-38330952 | None:intergenic | 50.0% |
| ! | GGAAAGATCGACTTCTTCGG+CGG | - | Chr1:38330818-38330837 | None:intergenic | 50.0% |
| !! | ATTTTCGAGGACGATGCCGT+CGG | - | Chr1:38330920-38330939 | None:intergenic | 50.0% |
| CGAGGACGTGAGAGATGAAG+TGG | - | Chr1:38330882-38330901 | None:intergenic | 55.0% | |
| TCCGAAGCACGTGCCTTCTA+TGG | + | Chr1:38330973-38330992 | MsG0180002428.01.T01:CDS | 55.0% | |
| TTCTTCGGCGGTCCAGAATG+AGG | - | Chr1:38330806-38330825 | None:intergenic | 55.0% | |
| TTGCCAGTGCTTGAGATCGG+AGG | - | Chr1:38330839-38330858 | None:intergenic | 55.0% | |
| AGGCACGTGCTTCGGAGACT+TGG | - | Chr1:38330969-38330988 | None:intergenic | 60.0% | |
| AGTGGCGTTCGCCGTCTGTT+AGG | - | Chr1:38330864-38330883 | None:intergenic | 60.0% | |
| GGCGGTCCAGAATGAGGCTT+CGG | - | Chr1:38330800-38330819 | None:intergenic | 60.0% | |
| TTCGAGGACGATGCCGTCGG+AGG | - | Chr1:38330917-38330936 | None:intergenic | 65.0% | |
| GAGGACGATGCCGTCGGAGG+CGG | - | Chr1:38330914-38330933 | None:intergenic | 70.0% | |
| GCCTTCTTCGCCGCCTCCGA+CGG | + | Chr1:38330901-38330920 | MsG0180002428.01.T01:CDS | 70.0% | |
| CGGAGGCGGCGAAGAAGGCG+AGG | - | Chr1:38330900-38330919 | None:intergenic | 75.0% | |
| GCCGTCGGAGGCGGCGAAGA+AGG | - | Chr1:38330905-38330924 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 38330751 | 38331059 | 38330751 | ID=MsG0180002428.01;Name=MsG0180002428.01 |
| Chr1 | mRNA | 38330751 | 38331059 | 38330751 | ID=MsG0180002428.01.T01;Parent=MsG0180002428.01;Name=MsG0180002428.01.T01;_AED=0.43;_eAED=0.43;_QI=0|-1|0|1|-1|1|1|0|102 |
| Chr1 | exon | 38330751 | 38331059 | 38330751 | ID=MsG0180002428.01.T01:exon:12916;Parent=MsG0180002428.01.T01 |
| Chr1 | CDS | 38330751 | 38331059 | 38330751 | ID=MsG0180002428.01.T01:cds;Parent=MsG0180002428.01.T01 |
| Gene Sequence |
| Protein sequence |
