AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380012956.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380012956.01.T01 MTR_3g063110 98.000 150 3 0 1 150 1 150 2.00e-103 292
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380012956.01.T01 AT3G46940 87.234 141 18 0 10 150 26 166 9.52e-87 251
MsG0380012956.01.T01 AT3G46940 87.234 141 18 0 10 150 67 207 9.82e-87 252

Find 46 sgRNAs with CRISPR-Local

Find 62 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCTGAAATTTCTGAGGTTT+TGG 0.210645 3:+27647432 MsG0380012956.01.T01:CDS
GTTAGAGGTGAAGGTGGTTT+TGG 0.293840 3:+27647469 MsG0380012956.01.T01:CDS
GATTTGGATTCTACTGTTAG+AGG 0.343621 3:+27647454 MsG0380012956.01.T01:CDS
TTTCTTCACTCGGAGAAAAC+TGG 0.351836 3:-27646998 None:intergenic
ACACGAGCATAGGTTCCTTC+AGG 0.369361 3:-27647153 None:intergenic
TCCAAAACCTCAGAAATTTC+AGG 0.370463 3:-27647433 None:intergenic
AACCTTTGAATCGGCGGCGC+TGG 0.374420 3:-27647082 None:intergenic
TCAACAATGTCCAACTTAAA+CGG 0.388632 3:+27646964 None:intergenic
CTGATTACAGAGGACCTGTT+GGG 0.415838 3:+27647323 MsG0380012956.01.T01:CDS
CATTCGATTGACGTTGGTGC+TGG 0.432456 3:+27647289 MsG0380012956.01.T01:CDS
ATGAGAGCTTTGCCGCGAGC+AGG 0.461473 3:-27647105 None:intergenic
TGAAACGACGCCGTTTAAGT+TGG 0.465891 3:-27646974 None:intergenic
CGCCGAGATCGGGGTTGACA+TGG 0.473074 3:+27647263 MsG0380012956.01.T01:CDS
TTCCATGTCAACCCCGATCT+CGG 0.481839 3:-27647265 None:intergenic
TTAAACAGTATAACCCCAAC+AGG 0.497604 3:-27647337 None:intergenic
CGCGGATCTCCTCTCTCCGC+CGG 0.497937 3:+27647048 MsG0380012956.01.T01:CDS
GGAAGGTATAACGGCTTTGT+CGG 0.505146 3:-27647025 None:intergenic
GATTCTACTGTTAGAGGTGA+AGG 0.505946 3:+27647460 MsG0380012956.01.T01:CDS
TGTCGGAGAGTTTCTTCACT+CGG 0.507441 3:-27647008 None:intergenic
CTCCAGCGCCGCCGATTCAA+AGG 0.509909 3:+27647080 MsG0380012956.01.T01:CDS
CTCTCCATCGCCGTCCCTGA+AGG 0.513836 3:+27647138 MsG0380012956.01.T01:CDS
GGCGCTGGAGAGATCGTATC+CGG 0.519854 3:-27647067 None:intergenic
CACGAGCATAGGTTCCTTCA+GGG 0.535140 3:-27647152 None:intergenic
AGATCCGCGGGAAGGTATAA+CGG 0.535522 3:-27647034 None:intergenic
GGCGGAGAGAGGAGATCCGC+GGG 0.537040 3:-27647046 None:intergenic
GCGAGCAGGAACCTTTGAAT+CGG 0.540643 3:-27647091 None:intergenic
ACGGCGATGGAGAGATCTGT+GGG 0.554559 3:-27647129 None:intergenic
GGTTCCTTCAGGGACGGCGA+TGG 0.559917 3:-27647142 None:intergenic
TATTACTCCTGAAATTTCTG+AGG 0.568921 3:+27647426 MsG0380012956.01.T01:CDS
TCTACTGTTAGAGGTGAAGG+TGG 0.578300 3:+27647463 MsG0380012956.01.T01:CDS
GACGGCGATGGAGAGATCTG+TGG 0.578622 3:-27647130 None:intergenic
AGCAGGAACCTTTGAATCGG+CGG 0.581168 3:-27647088 None:intergenic
GTAATTACCAACACGAGCAT+AGG 0.588762 3:-27647163 None:intergenic
AGATCGTATCCGGCGGAGAG+AGG 0.592423 3:-27647057 None:intergenic
GAAGGAACCTATGCTCGTGT+TGG 0.606547 3:+27647156 MsG0380012956.01.T01:CDS
GCTGATTACAGAGGACCTGT+TGG 0.614644 3:+27647322 MsG0380012956.01.T01:CDS
TGGAAGCATTCGATTGACGT+TGG 0.614719 3:+27647283 MsG0380012956.01.T01:CDS
GAGAGAGGAGATCCGCGGGA+AGG 0.626530 3:-27647042 None:intergenic
AGCATAGGTTCCTTCAGGGA+CGG 0.629807 3:-27647148 None:intergenic
GATTCAAAGGTTCCTGCTCG+CGG 0.632457 3:+27647093 MsG0380012956.01.T01:CDS
CGGCGGAGAGAGGAGATCCG+CGG 0.639931 3:-27647047 None:intergenic
TGATTACAGAGGACCTGTTG+GGG 0.654205 3:+27647324 MsG0380012956.01.T01:CDS
AAAGCCGTTATACCTTCCCG+CGG 0.736814 3:+27647030 MsG0380012956.01.T01:CDS
GTGATTGATGCTGATTACAG+AGG 0.740502 3:+27647313 MsG0380012956.01.T01:CDS
GCTGGAGAGATCGTATCCGG+CGG 0.743244 3:-27647064 None:intergenic
CGGCGATGGAGAGATCTGTG+GGG 0.756952 3:-27647128 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAACCCTAAAATTCAAAAT+TGG - Chr3:27647203-27647222 None:intergenic 20.0%
!! AAACCCTAAAATTCAAAATT+GGG - Chr3:27647202-27647221 None:intergenic 20.0%
!! TAAAATTCAAAATTGGGAAA+GGG - Chr3:27647196-27647215 None:intergenic 20.0%
!!! ATTTTAGGGTTTTGAAATTT+AGG + Chr3:27647210-27647229 MsG0380012956.01.T01:intron 20.0%
!!! TTTCCCAATTTTGAATTTTA+GGG + Chr3:27647196-27647215 MsG0380012956.01.T01:intron 20.0%
!!! TTTTAGGGTTTTGAAATTTA+GGG + Chr3:27647211-27647230 MsG0380012956.01.T01:intron 20.0%
! AAAATTCAAAATTGGGAAAG+GGG - Chr3:27647195-27647214 None:intergenic 25.0%
! CTAAAATTCAAAATTGGGAA+AGG - Chr3:27647197-27647216 None:intergenic 25.0%
!! CTTTCCCAATTTTGAATTTT+AGG + Chr3:27647195-27647214 MsG0380012956.01.T01:intron 25.0%
TATTACTCCTGAAATTTCTG+AGG + Chr3:27647426-27647445 MsG0380012956.01.T01:CDS 30.0%
!!! AATTTCTGAGGTTTTGGATT+TGG + Chr3:27647438-27647457 MsG0380012956.01.T01:CDS 30.0%
!!! GTTGATTTTGAAGTGAAAGT+TGG + Chr3:27647370-27647389 MsG0380012956.01.T01:CDS 30.0%
GATTTGGATTCTACTGTTAG+AGG + Chr3:27647454-27647473 MsG0380012956.01.T01:CDS 35.0%
TCCAAAACCTCAGAAATTTC+AGG - Chr3:27647436-27647455 None:intergenic 35.0%
TTAAACAGTATAACCCCAAC+AGG - Chr3:27647340-27647359 None:intergenic 35.0%
!!! TCCTGAAATTTCTGAGGTTT+TGG + Chr3:27647432-27647451 MsG0380012956.01.T01:CDS 35.0%
GATTCTACTGTTAGAGGTGA+AGG + Chr3:27647460-27647479 MsG0380012956.01.T01:CDS 40.0%
GTAATTACCAACACGAGCAT+AGG - Chr3:27647166-27647185 None:intergenic 40.0%
TTTCTTCACTCGGAGAAAAC+TGG - Chr3:27647001-27647020 None:intergenic 40.0%
! GTGATTGATGCTGATTACAG+AGG + Chr3:27647313-27647332 MsG0380012956.01.T01:CDS 40.0%
!!! AAGGTGGTTTTGGATCTACT+GGG + Chr3:27647479-27647498 MsG0380012956.01.T01:CDS 40.0%
CTGATTACAGAGGACCTGTT+GGG + Chr3:27647323-27647342 MsG0380012956.01.T01:CDS 45.0%
TCTACTGTTAGAGGTGAAGG+TGG + Chr3:27647463-27647482 MsG0380012956.01.T01:CDS 45.0%
TGAAACGACGCCGTTTAAGT+TGG - Chr3:27646977-27646996 None:intergenic 45.0%
TGATTACAGAGGACCTGTTG+GGG + Chr3:27647324-27647343 MsG0380012956.01.T01:CDS 45.0%
TGTCGGAGAGTTTCTTCACT+CGG - Chr3:27647011-27647030 None:intergenic 45.0%
! GGAAGGTATAACGGCTTTGT+CGG - Chr3:27647028-27647047 None:intergenic 45.0%
! GTTAGAGGTGAAGGTGGTTT+TGG + Chr3:27647469-27647488 MsG0380012956.01.T01:CDS 45.0%
!! TGGAAGCATTCGATTGACGT+TGG + Chr3:27647283-27647302 MsG0380012956.01.T01:CDS 45.0%
!!! AGGTGGTTTTGGATCTACTG+GGG + Chr3:27647480-27647499 MsG0380012956.01.T01:CDS 45.0%
!!! GAAGGTGGTTTTGGATCTAC+TGG + Chr3:27647478-27647497 MsG0380012956.01.T01:CDS 45.0%
AAAGCCGTTATACCTTCCCG+CGG + Chr3:27647030-27647049 MsG0380012956.01.T01:CDS 50.0%
ACACGAGCATAGGTTCCTTC+AGG - Chr3:27647156-27647175 None:intergenic 50.0%
AGCAGGAACCTTTGAATCGG+CGG - Chr3:27647091-27647110 None:intergenic 50.0%
AGCATAGGTTCCTTCAGGGA+CGG - Chr3:27647151-27647170 None:intergenic 50.0%
CACGAGCATAGGTTCCTTCA+GGG - Chr3:27647155-27647174 None:intergenic 50.0%
CATTCGATTGACGTTGGTGC+TGG + Chr3:27647289-27647308 MsG0380012956.01.T01:CDS 50.0%
GAAGGAACCTATGCTCGTGT+TGG + Chr3:27647156-27647175 MsG0380012956.01.T01:CDS 50.0%
GATTCAAAGGTTCCTGCTCG+CGG + Chr3:27647093-27647112 MsG0380012956.01.T01:CDS 50.0%
GCGAGCAGGAACCTTTGAAT+CGG - Chr3:27647094-27647113 None:intergenic 50.0%
GCTGATTACAGAGGACCTGT+TGG + Chr3:27647322-27647341 MsG0380012956.01.T01:CDS 50.0%
TTCCATGTCAACCCCGATCT+CGG - Chr3:27647268-27647287 None:intergenic 50.0%
! AGATCCGCGGGAAGGTATAA+CGG - Chr3:27647037-27647056 None:intergenic 50.0%
!! ATTTTTGCAGCGCCGAGATC+GGG + Chr3:27647253-27647272 MsG0380012956.01.T01:intron 50.0%
!! GATTTTTGCAGCGCCGAGAT+CGG + Chr3:27647252-27647271 MsG0380012956.01.T01:intron 50.0%
ACGGCGATGGAGAGATCTGT+GGG - Chr3:27647132-27647151 None:intergenic 55.0%
!! TTTTTGCAGCGCCGAGATCG+GGG + Chr3:27647254-27647273 MsG0380012956.01.T01:intron 55.0%
AACCTTTGAATCGGCGGCGC+TGG - Chr3:27647085-27647104 None:intergenic 60.0%
AGATCGTATCCGGCGGAGAG+AGG - Chr3:27647060-27647079 None:intergenic 60.0%
ATGAGAGCTTTGCCGCGAGC+AGG - Chr3:27647108-27647127 None:intergenic 60.0%
CGGCGATGGAGAGATCTGTG+GGG - Chr3:27647131-27647150 None:intergenic 60.0%
GACGGCGATGGAGAGATCTG+TGG - Chr3:27647133-27647152 None:intergenic 60.0%
GCTGGAGAGATCGTATCCGG+CGG - Chr3:27647067-27647086 None:intergenic 60.0%
! GGCGCTGGAGAGATCGTATC+CGG - Chr3:27647070-27647089 None:intergenic 60.0%
CGCCGAGATCGGGGTTGACA+TGG + Chr3:27647263-27647282 MsG0380012956.01.T01:CDS 65.0%
CTCTCCATCGCCGTCCCTGA+AGG + Chr3:27647138-27647157 MsG0380012956.01.T01:CDS 65.0%
GAGAGAGGAGATCCGCGGGA+AGG - Chr3:27647045-27647064 None:intergenic 65.0%
GGTTCCTTCAGGGACGGCGA+TGG - Chr3:27647145-27647164 None:intergenic 65.0%
!! CTCCAGCGCCGCCGATTCAA+AGG + Chr3:27647080-27647099 MsG0380012956.01.T01:CDS 65.0%
CGCGGATCTCCTCTCTCCGC+CGG + Chr3:27647048-27647067 MsG0380012956.01.T01:CDS 70.0%
CGGCGGAGAGAGGAGATCCG+CGG - Chr3:27647050-27647069 None:intergenic 70.0%
GGCGGAGAGAGGAGATCCGC+GGG - Chr3:27647049-27647068 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
Chr3 gene 27646970 27647507 27646970 ID=MsG0380012956.01;Name=MsG0380012956.01
Chr3 mRNA 27646970 27647507 27646970 ID=MsG0380012956.01.T01;Parent=MsG0380012956.01;Name=MsG0380012956.01.T01;_AED=0.50;_eAED=0.52;_QI=0|0|0|1|1|1|2|0|150
Chr3 exon 27646970 27647177 27646970 ID=MsG0380012956.01.T01:exon:24062;Parent=MsG0380012956.01.T01
Chr3 exon 27647263 27647507 27647263 ID=MsG0380012956.01.T01:exon:24063;Parent=MsG0380012956.01.T01
Chr3 CDS 27646970 27647177 27646970 ID=MsG0380012956.01.T01:cds;Parent=MsG0380012956.01.T01
Chr3 CDS 27647263 27647507 27647263 ID=MsG0380012956.01.T01:cds;Parent=MsG0380012956.01.T01
Gene Sequence

>MsG0380012956.01.T01

ATGTCCAACTTAAACGGCGTCGTTTCATCCAGTTTTCTCCGAGTGAAGAAACTCTCCGACAAAGCCGTTATACCTTCCCGCGGATCTCCTCTCTCCGCCGGATACGATCTCTCCAGCGCCGCCGATTCAAAGGTTCCTGCTCGCGGCAAAGCTCTCATCCCCACAGATCTCTCCATCGCCGTCCCTGAAGGAACCTATGCTCGTGTTGCGCCGAGATCGGGGTTGACATGGAAGCATTCGATTGACGTTGGTGCTGGAGTGATTGATGCTGATTACAGAGGACCTGTTGGGGTTATACTGTTTAATCATTCAGATGTTGATTTTGAAGTGAAAGTTGGTGATAGAGTTGCTCAGTTGATTATTGAGAAGATTATTACTCCTGAAATTTCTGAGGTTTTGGATTTGGATTCTACTGTTAGAGGTGAAGGTGGTTTTGGATCTACTGGGGTTTGA

Protein sequence

>MsG0380012956.01.T01

MSNLNGVVSSSFLRVKKLSDKAVIPSRGSPLSAGYDLSSAADSKVPARGKALIPTDLSIAVPEGTYARVAPRSGLTWKHSIDVGAGVIDADYRGPVGVILFNHSDVDFEVKVGDRVAQLIIEKIITPEISEVLDLDSTVRGEGGFGSTGV*