AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380013892.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380013892.01.T01 MTR_4g451295 100.000 82 0 0 1 82 221 302 5.31e-55 172
MsG0380013892.01.T01 MTR_5g080940 97.531 81 2 0 1 81 291 371 1.77e-50 164
MsG0380013892.01.T01 MTR_4g006070 100.000 64 0 0 18 81 235 298 9.19e-40 134
MsG0380013892.01.T01 MTR_1g039020 94.545 55 3 0 28 82 8 62 4.38e-33 108
MsG0380013892.01.T01 MTR_4g076920 100.000 46 0 0 20 65 132 177 1.11e-26 97.4
MsG0380013892.01.T01 MTR_4g122520 60.417 48 17 1 36 81 5 52 3.72e-13 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 41 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCACGAAAGGCGTAACGATC+TGG 0.311741 3:+47530003 None:intergenic
CACCAACTTCCTTAACCTTC+CGG 0.362818 3:-47529878 MsG0380013892.01.T01:CDS
TTCACCGGGGCTTCGGTCGC+CGG 0.371598 3:-47529918 MsG0380013892.01.T01:CDS
CAAAGTCGTAAGACCATGTA+TGG 0.380055 3:+47529835 None:intergenic
GCCGCCGTTCACCGGGGCTT+CGG 0.397671 3:-47529925 MsG0380013892.01.T01:CDS
TGCCCAGTGCCGGAAGGTTA+AGG 0.409134 3:+47529869 None:intergenic
CTTCCTTAACCTTCCGGCAC+TGG 0.422146 3:-47529872 MsG0380013892.01.T01:CDS
TAGTTACGGCCGCCGTTCAC+CGG 0.432611 3:-47529933 MsG0380013892.01.T01:CDS
GGCTGACGCCTGCCCAGTGC+CGG 0.435469 3:+47529859 None:intergenic
TTAACCTTCCGGCACTGGGC+AGG 0.436659 3:-47529867 MsG0380013892.01.T01:CDS
AGTTACGGCCGCCGTTCACC+GGG 0.437053 3:-47529932 MsG0380013892.01.T01:CDS
TCGCCGGCTTCCCTGTCATC+AGG 0.453580 3:-47529902 MsG0380013892.01.T01:CDS
GAAGCCGGCGACCGAAGCCC+CGG 0.461073 3:+47529914 None:intergenic
TACATGGTCTTACGACTTTG+CGG 0.476001 3:-47529832 MsG0380013892.01.T01:CDS
TGCCGGAAGGTTAAGGAAGT+TGG 0.485164 3:+47529876 None:intergenic
ACAAGGAATTTCGCTACCTT+AGG 0.488888 3:-47529963 MsG0380013892.01.T01:CDS
CACGAAAGGCGTAACGATCT+GGG 0.491297 3:+47530004 None:intergenic
GAAGTTGGTGACCTGATGAC+AGG 0.495416 3:+47529891 None:intergenic
AGGTAGCGAAATTCCTTGTC+GGG 0.495901 3:+47529967 None:intergenic
TTCCTTAACCTTCCGGCACT+GGG 0.504342 3:-47529871 MsG0380013892.01.T01:CDS
GCGACCGAAGCCCCGGTGAA+CGG 0.505900 3:+47529921 None:intergenic
CACTGTCTCGGAGAGAGGCT+CGG 0.506062 3:+47530027 None:intergenic
AAGGTAGCGAAATTCCTTGT+CGG 0.511527 3:+47529966 None:intergenic
TAACGATCTGGGCACTGTCT+CGG 0.513539 3:+47530015 None:intergenic
GATCGTTACGCCTTTCGTGC+GGG 0.516913 3:-47530000 MsG0380013892.01.T01:CDS
TGACCTGATGACAGGGAAGC+CGG 0.518242 3:+47529899 None:intergenic
CCTTAGGACCGTTATAGTTA+CGG 0.519214 3:-47529947 MsG0380013892.01.T01:CDS
CTGGGCACTGTCTCGGAGAG+AGG 0.542198 3:+47530022 None:intergenic
AAAGTCGTAAGACCATGTAT+GGG 0.556989 3:+47529836 None:intergenic
ACCGAAGCCCCGGTGAACGG+CGG 0.564471 3:+47529924 None:intergenic
AGATCGTTACGCCTTTCGTG+CGG 0.565799 3:-47530001 MsG0380013892.01.T01:CDS
GACGCCTGCCCAGTGCCGGA+AGG 0.568365 3:+47529863 None:intergenic
CCGTAACTATAACGGTCCTA+AGG 0.574485 3:+47529947 None:intergenic
TAAGTTCCGACCCGCACGAA+AGG 0.581321 3:+47529990 None:intergenic
AACGGCGGCCGTAACTATAA+CGG 0.599287 3:+47529939 None:intergenic
GTTACGCCTTTCGTGCGGGT+CGG 0.605304 3:-47529996 MsG0380013892.01.T01:CDS
AAGTCGTAAGACCATGTATG+GGG 0.621859 3:+47529837 None:intergenic
AAGTTGGTGACCTGATGACA+GGG 0.627282 3:+47529892 None:intergenic
GGGTCGGAACTTACCCGACA+AGG 0.655880 3:-47529980 MsG0380013892.01.T01:CDS
AGTCGTAAGACCATGTATGG+GGG 0.686647 3:+47529838 None:intergenic
GTTACGGCCGCCGTTCACCG+GGG 0.732311 3:-47529931 MsG0380013892.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AAAGTCGTAAGACCATGTAT+GGG + Chr3:47530019-47530038 None:intergenic 35.0%
AAGGTAGCGAAATTCCTTGT+CGG + Chr3:47529889-47529908 None:intergenic 40.0%
AAGTCGTAAGACCATGTATG+GGG + Chr3:47530018-47530037 None:intergenic 40.0%
ACAAGGAATTTCGCTACCTT+AGG - Chr3:47529889-47529908 MsG0380013892.01.T01:CDS 40.0%
CAAAGTCGTAAGACCATGTA+TGG + Chr3:47530020-47530039 None:intergenic 40.0%
CCTTAGGACCGTTATAGTTA+CGG - Chr3:47529905-47529924 MsG0380013892.01.T01:CDS 40.0%
! TACATGGTCTTACGACTTTG+CGG - Chr3:47530020-47530039 MsG0380013892.01.T01:CDS 40.0%
AGGTAGCGAAATTCCTTGTC+GGG + Chr3:47529888-47529907 None:intergenic 45.0%
AGTCGTAAGACCATGTATGG+GGG + Chr3:47530017-47530036 None:intergenic 45.0%
CACCAACTTCCTTAACCTTC+CGG - Chr3:47529974-47529993 MsG0380013892.01.T01:CDS 45.0%
CCGTAACTATAACGGTCCTA+AGG + Chr3:47529908-47529927 None:intergenic 45.0%
! TTTGCGGAGACCTGTGTTTT+TGG - Chr3:47530036-47530055 MsG0380013892.01.T01:CDS 45.0%
!! AAGTTGGTGACCTGATGACA+GGG + Chr3:47529963-47529982 None:intergenic 45.0%
AACGGCGGCCGTAACTATAA+CGG + Chr3:47529916-47529935 None:intergenic 50.0%
AGATCGTTACGCCTTTCGTG+CGG - Chr3:47529851-47529870 MsG0380013892.01.T01:CDS 50.0%
TGCCGGAAGGTTAAGGAAGT+TGG + Chr3:47529979-47529998 None:intergenic 50.0%
TTCCTTAACCTTCCGGCACT+GGG - Chr3:47529981-47530000 MsG0380013892.01.T01:CDS 50.0%
! CACGAAAGGCGTAACGATCT+GGG + Chr3:47529851-47529870 None:intergenic 50.0%
! TAACGATCTGGGCACTGTCT+CGG + Chr3:47529840-47529859 None:intergenic 50.0%
!! GAAGTTGGTGACCTGATGAC+AGG + Chr3:47529964-47529983 None:intergenic 50.0%
CTTCCTTAACCTTCCGGCAC+TGG - Chr3:47529980-47529999 MsG0380013892.01.T01:CDS 55.0%
GATCGTTACGCCTTTCGTGC+GGG - Chr3:47529852-47529871 MsG0380013892.01.T01:CDS 55.0%
TAAGTTCCGACCCGCACGAA+AGG + Chr3:47529865-47529884 None:intergenic 55.0%
TGACCTGATGACAGGGAAGC+CGG + Chr3:47529956-47529975 None:intergenic 55.0%
! GCACGAAAGGCGTAACGATC+TGG + Chr3:47529852-47529871 None:intergenic 55.0%
CACTGTCTCGGAGAGAGGCT+CGG + Chr3:47529828-47529847 None:intergenic 60.0%
GGGTCGGAACTTACCCGACA+AGG - Chr3:47529872-47529891 MsG0380013892.01.T01:CDS 60.0%
GTTACGCCTTTCGTGCGGGT+CGG - Chr3:47529856-47529875 MsG0380013892.01.T01:CDS 60.0%
TAGTTACGGCCGCCGTTCAC+CGG - Chr3:47529919-47529938 MsG0380013892.01.T01:CDS 60.0%
! TGCCCAGTGCCGGAAGGTTA+AGG + Chr3:47529986-47530005 None:intergenic 60.0%
! TTAACCTTCCGGCACTGGGC+AGG - Chr3:47529985-47530004 MsG0380013892.01.T01:CDS 60.0%
AGTTACGGCCGCCGTTCACC+GGG - Chr3:47529920-47529939 MsG0380013892.01.T01:CDS 65.0%
CAGGCGTCAGCCCCCATACA+TGG - Chr3:47530004-47530023 MsG0380013892.01.T01:CDS 65.0%
! CTGGGCACTGTCTCGGAGAG+AGG + Chr3:47529833-47529852 None:intergenic 65.0%
!! TCGCCGGCTTCCCTGTCATC+AGG - Chr3:47529950-47529969 MsG0380013892.01.T01:CDS 65.0%
ACCGAAGCCCCGGTGAACGG+CGG + Chr3:47529931-47529950 None:intergenic 70.0%
GCGACCGAAGCCCCGGTGAA+CGG + Chr3:47529934-47529953 None:intergenic 70.0%
GTTACGGCCGCCGTTCACCG+GGG - Chr3:47529921-47529940 MsG0380013892.01.T01:CDS 70.0%
TTCACCGGGGCTTCGGTCGC+CGG - Chr3:47529934-47529953 MsG0380013892.01.T01:CDS 70.0%
GAAGCCGGCGACCGAAGCCC+CGG + Chr3:47529941-47529960 None:intergenic 75.0%
GCCGCCGTTCACCGGGGCTT+CGG - Chr3:47529927-47529946 MsG0380013892.01.T01:CDS 75.0%
GGCTGACGCCTGCCCAGTGC+CGG + Chr3:47529996-47530015 None:intergenic 75.0%
! GACGCCTGCCCAGTGCCGGA+AGG + Chr3:47529992-47530011 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
Chr3 gene 47529813 47530061 47529813 ID=MsG0380013892.01;Name=MsG0380013892.01
Chr3 mRNA 47529813 47530061 47529813 ID=MsG0380013892.01.T01;Parent=MsG0380013892.01;Name=MsG0380013892.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|0|1|0|82
Chr3 exon 47529813 47530061 47529813 ID=MsG0380013892.01.T01:exon:26403;Parent=MsG0380013892.01.T01
Chr3 CDS 47529813 47530061 47529813 ID=MsG0380013892.01.T01:cds;Parent=MsG0380013892.01.T01
Gene Sequence

>MsG0380013892.01.T01

ATGTCTATTTCACCGAGCCTCTCTCCGAGACAGTGCCCAGATCGTTACGCCTTTCGTGCGGGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGACCGTTATAGTTACGGCCGCCGTTCACCGGGGCTTCGGTCGCCGGCTTCCCTGTCATCAGGTCACCAACTTCCTTAACCTTCCGGCACTGGGCAGGCGTCAGCCCCCATACATGGTCTTACGACTTTGCGGAGACCTGTGTTTTTGGTAA

Protein sequence

>MsG0380013892.01.T01

MSISPSLSPRQCPDRYAFRAGRNLPDKEFRYLRTVIVTAAVHRGFGRRLPCHQVTNFLNLPALGRRQPPYMVLRLCGDLCFW*