AlfalfaGEDB | MsG0180004793.01

M. sativa cultivar ZhongmuNo.1 / MsG0180004793.01

KEGG: -
GO: GO:0003700 GO:0006355
PFAM: PF00170

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180004793.01.T01	XP_003591402.1	96.667	150	5	0	1	150	1	150	1.26E-101	298

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180004793.01.T01	Q9LZP8	45.614	114	59	1	25	138	23	133	2.62E-26	99.8

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180004793.01.T01	G7IDQ2	96.667	150	5	0	1	150	1	150	6.02e-102	298

TFs/TRs:

Gene ID	Type	Classification
MsG0180004793.01.T01	TF	bZIP

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048178.01	MsG0180004793.01	0.811048	8.504678e-51	5.210812e-48
MsG0180000197.01	MsG0180004793.01	0.832290	1.028643e-55	1.144543e-52
MsG0180002075.01	MsG0180004793.01	0.805201	1.496266e-49	7.868159e-47
MsG0180004388.01	MsG0180004793.01	0.801851	7.409544e-49	3.575734e-46
MsG0180004689.01	MsG0180004793.01	0.813502	2.477373e-51	1.620734e-48
MsG0180004793.01	MsG0180005125.01	0.801148	1.032222e-48	4.893724e-46
MsG0180004793.01	MsG0180005944.01	0.806527	7.876836e-50	4.285022e-47
MsG0180004793.01	MsG0180006106.01	0.819583	1.076346e-52	8.316505e-50
MsG0180004793.01	MsG0280007481.01	0.820526	6.546943e-53	5.192699e-50
MsG0180004793.01	MsG0280007609.01	0.807772	4.293941e-50	2.412470e-47
MsG0180004793.01	MsG0280007764.01	0.809974	1.451368e-50	8.643016e-48
MsG0180004793.01	MsG0280008220.01	0.816673	4.899299e-52	3.493907e-49
MsG0180004793.01	MsG0280008270.01	0.805020	1.633182e-49	8.547915e-47
MsG0180004793.01	MsG0280008295.01	0.809193	2.135254e-50	1.245541e-47
MsG0180004793.01	MsG0280009235.01	0.822976	1.775762e-53	1.509189e-50
MsG0180004793.01	MsG0280009610.01	0.835783	1.372552e-56	1.695833e-53
MsG0180004793.01	MsG0280009996.01	0.804865	1.759042e-49	9.171027e-47
MsG0180004793.01	MsG0280010614.01	0.803835	2.884374e-49	1.464335e-46
MsG0180004793.01	MsG0280011105.01	0.882425	1.053442e-70	6.412943e-67
MsG0180004793.01	MsG0280011235.01	0.805137	1.543581e-49	8.103522e-47
MsG0180004793.01	MsG0280011410.01	0.809070	2.268990e-50	1.319166e-47
MsG0180004793.01	MsG0380011754.01	0.809962	1.460039e-50	8.692156e-48
MsG0180004793.01	MsG0380014163.01	0.813237	2.833099e-51	1.839904e-48
MsG0180004793.01	MsG0380014680.01	0.816393	5.660932e-52	4.006268e-49
MsG0180004793.01	MsG0380014913.01	0.842339	2.747471e-58	4.152924e-55
MsG0180004793.01	MsG0380016227.01	0.840665	7.584192e-58	1.087905e-54
MsG0180004793.01	MsG0380017604.01	0.835491	1.627022e-56	1.992773e-53
MsG0180004793.01	MsG0380017631.01	0.831706	1.433565e-55	1.568261e-52
MsG0180004793.01	MsG0480018787.01	0.830187	3.381942e-55	3.537564e-52
MsG0180004793.01	MsG0480018970.01	0.817758	2.792764e-52	2.051822e-49
MsG0180004793.01	MsG0480020242.01	0.819900	9.110194e-53	7.101574e-50
MsG0180004793.01	MsG0480020964.01	0.803743	3.014004e-49	1.526524e-46
MsG0180004793.01	MsG0480022406.01	0.814540	1.462677e-51	9.841574e-49
MsG0180004793.01	MsG0480022451.01	0.801828	7.488470e-49	3.611764e-46
MsG0180004793.01	MsG0480022480.01	0.821807	3.317657e-53	2.727423e-50
MsG0180004793.01	MsG0480022533.01	0.800565	1.358125e-48	6.344040e-46
MsG0180004793.01	MsG0480022597.01	0.845106	4.995074e-59	8.235549e-56
MsG0180004793.01	MsG0480022598.01	0.819027	1.440419e-52	1.095991e-49
MsG0180004793.01	MsG0480022661.01	0.800491	1.406022e-48	6.555552e-46
MsG0180004793.01	MsG0480023248.01	0.804622	1.977767e-49	1.024547e-46
MsG0180004793.01	MsG0580024733.01	0.802288	6.023916e-49	2.939659e-46
MsG0180004793.01	MsG0580026595.01	0.805128	1.550226e-49	8.136606e-47
MsG0180004793.01	MsG0580027129.01	0.805710	1.170708e-49	6.237458e-47
MsG0180004793.01	MsG0580027532.01	0.805765	1.139620e-49	6.080393e-47
MsG0180004793.01	MsG0580028453.01	0.832415	9.575567e-56	1.069591e-52
MsG0180004793.01	MsG0580028969.01	0.806834	6.784777e-50	3.720275e-47
MsG0180004793.01	MsG0580028980.01	0.835183	1.946749e-56	2.362390e-53
MsG0180004793.01	MsG0580030235.01	0.821184	4.621801e-53	3.733722e-50
MsG0180004793.01	MsG0680030685.01	0.816076	6.661346e-52	4.673853e-49
MsG0180004793.01	MsG0680031538.01	0.802226	6.202716e-49	3.022006e-46
MsG0180004793.01	MsG0680031719.01	0.808240	3.413075e-50	1.941468e-47
MsG0180004793.01	MsG0680034036.01	0.802926	4.448928e-49	2.206569e-46
MsG0180004793.01	MsG0680035656.01	0.822546	2.235432e-53	1.876959e-50
MsG0180004793.01	MsG0680035663.01	0.806329	8.671375e-50	4.693596e-47
MsG0180004793.01	MsG0780036630.01	0.835434	1.681956e-56	2.056524e-53
MsG0180004793.01	MsG0780036635.01	0.833713	4.552472e-56	5.285731e-53
MsG0180004793.01	MsG0780037975.01	0.806588	7.648823e-50	4.167356e-47
MsG0180004793.01	MsG0780038117.01	0.815983	6.990093e-52	4.891786e-49
MsG0180004793.01	MsG0780038829.01	0.806260	8.969393e-50	4.846490e-47
MsG0180004793.01	MsG0780040505.01	0.824782	6.698473e-54	5.992709e-51
MsG0180004793.01	MsG0780040516.01	0.856976	2.234518e-62	5.429653e-59
MsG0180004793.01	MsG0780040679.01	0.825114	5.591087e-54	5.049592e-51
MsG0180004793.01	MsG0780040726.01	0.805065	1.597756e-49	8.372578e-47
MsG0180004793.01	MsG0780041485.01	0.804428	2.170818e-49	1.118936e-46
MsG0180004793.01	MsG0780041777.01	0.819535	1.103754e-52	8.517021e-50
MsG0180004793.01	MsG0880042811.01	0.814274	1.674572e-51	1.118647e-48
MsG0180004793.01	MsG0880043289.01	0.802724	4.898377e-49	2.417093e-46
MsG0180004793.01	MsG0880043489.01	0.833705	4.573992e-56	5.309402e-53
MsG0180004793.01	MsG0880045518.01	0.853219	2.761220e-61	5.922853e-58
MsG0180004793.01	MsG0880045897.01	-0.803330	3.671160e-49	1.839639e-46
MsG0180004793.01	MsG0880045914.01	0.816457	5.476602e-52	3.882364e-49
MsG0180004793.01	MsG0880046244.01	0.811512	6.745865e-51	4.184081e-48
MsG0180004793.01	MsG0880046561.01	0.805530	1.277018e-49	6.771833e-47
MsG0180004793.01	MsG0880047497.01	0.801175	1.019020e-48	4.834399e-46

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180004793.01.T01	MTR_1g087040	96.667	150	5	0	1	150	1	150	1.53e-105	298
MsG0180004793.01.T01	MTR_7g109510	48.993	149	63	2	1	149	1	136	6.47e-32	111
MsG0180004793.01.T01	MTR_4g070860	36.090	133	70	2	13	130	16	148	1.99e-23	90.1
MsG0180004793.01.T01	MTR_1g022495	38.816	152	83	5	7	149	9	159	2.75e-23	89.7
MsG0180004793.01.T01	MTR_3g117120	44.211	95	53	0	13	107	16	110	5.11e-23	89.0
MsG0180004793.01.T01	MTR_8g086020	36.986	146	82	4	1	138	1	144	5.19e-22	86.7
MsG0180004793.01.T01	MTR_3g112220	41.772	79	46	0	24	102	1	79	1.80e-15	67.8
MsG0180004793.01.T01	MTR_7g029400	41.935	93	50	2	7	95	60	152	4.85e-14	66.6
MsG0180004793.01.T01	MTR_8g015250	37.374	99	58	2	1	95	44	142	2.76e-13	64.3
MsG0180004793.01.T01	MTR_3g103360	35.000	100	65	0	7	106	36	135	3.61e-13	63.2
MsG0180004793.01.T01	MTR_5g015090	36.667	90	51	1	8	91	36	125	5.31e-13	63.2
MsG0180004793.01.T01	MTR_4g097440	32.292	96	62	1	24	119	72	164	5.56e-12	60.5
MsG0180004793.01.T01	MTR_7g017880	36.697	109	62	2	24	131	84	186	6.90e-12	60.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180004793.01.T01	AT3G62420	45.614	114	59	1	25	138	23	133	2.68e-27	99.8
MsG0180004793.01.T01	AT2G18160	45.545	101	54	1	22	122	26	125	6.27e-25	94.4
MsG0180004793.01.T01	AT1G75390	40.000	120	68	1	24	139	38	157	3.10e-23	90.1
MsG0180004793.01.T01	AT4G34590	48.810	84	43	0	24	107	24	107	3.93e-22	86.7
MsG0180004793.01.T01	AT1G75390	49.425	87	39	2	9	91	20	105	2.21e-19	78.6
MsG0180004793.01.T01	AT3G30530	42.466	73	42	0	24	96	78	150	1.98e-12	62.0
MsG0180004793.01.T01	AT5G38800	45.205	73	40	0	24	96	69	141	2.03e-12	61.6
MsG0180004793.01.T01	AT2G22850	43.038	79	44	1	22	99	123	201	2.15e-11	60.1
MsG0180004793.01.T01	AT2G22850	43.038	79	44	1	22	99	123	201	2.15e-11	60.1
MsG0180004793.01.T01	AT1G13600	37.273	110	64	2	8	117	67	171	4.08e-11	58.9

Find 32 sgRNAs with CRISPR-Local

Find 33 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGTGATGCCATAGAATAATT+TGG	0.207768	1:+82786005	None:intergenic
ACCTTATCCAAATTATTCTA+TGG	0.288233	1:-82786012	MsG0180004793.01.T01:CDS
CTGTTGTTGCTTCCTCAGTC+TGG	0.324562	1:+82786292	None:intergenic
GAACCTGAACCTGAACTTAC+AGG	0.327424	1:+82786398	None:intergenic
CCGTCTCGCGCGGAGTATTA+AGG	0.331337	1:-82786222	MsG0180004793.01.T01:CDS
GTAAGTTCAGGTTCAGGTTC+AGG	0.347729	1:-82786395	MsG0180004793.01.T01:CDS
ATGATCCTCTTCTCAAACCT+TGG	0.395871	1:-82786040	MsG0180004793.01.T01:CDS
CAGAGCGCAGACAAAAGAAT+TGG	0.420236	1:-82786153	MsG0180004793.01.T01:CDS
ATTCAACGTCCTGTAAGTTC+AGG	0.426014	1:-82786407	MsG0180004793.01.T01:CDS
CGTCCTGTAAGTTCAGGTTC+AGG	0.436352	1:-82786401	MsG0180004793.01.T01:CDS
AAGAATTGGAGGCTCAGTTT+CGG	0.458820	1:-82786139	MsG0180004793.01.T01:CDS
TTTCAATCTACCGGCTTCTC+CGG	0.462286	1:+82786256	None:intergenic
GCCATAGAATAATTTGGATA+AGG	0.470081	1:+82786011	None:intergenic
TTCAGGAGGTTCAGACGGTA+TGG	0.508548	1:-82786378	MsG0180004793.01.T01:CDS
GGAGGAAGCGTATGTGAAGA+TGG	0.510835	1:-82786195	MsG0180004793.01.T01:CDS
TGGATCGTCTCGCGGATTCA+CGG	0.514018	1:+82786312	None:intergenic
CAGACTGAGGAAGCAACAAC+AGG	0.520449	1:-82786291	MsG0180004793.01.T01:CDS
GAAGATCTGACCGGAGAAGC+CGG	0.539519	1:-82786266	MsG0180004793.01.T01:CDS
AGATGCTGCTGCTGCTGAAG+AGG	0.541104	1:-82786099	MsG0180004793.01.T01:CDS
CGCGCGGAGTATTAAGGCGA+CGG	0.554524	1:-82786216	MsG0180004793.01.T01:CDS
AGCGCAGACAAAAGAATTGG+AGG	0.555181	1:-82786150	MsG0180004793.01.T01:CDS
CCTTAATACTCCGCGCGAGA+CGG	0.575439	1:+82786222	None:intergenic
AGAAGAGGATCATCAATCAA+AGG	0.595685	1:+82786050	None:intergenic
ATGAACCAAGGTTTGAGAAG+AGG	0.601064	1:+82786035	None:intergenic
TTTGGATAAGGTATGAACCA+AGG	0.606535	1:+82786023	None:intergenic
AGTTCAGGTTCAGGTTCAGG+AGG	0.612664	1:-82786392	MsG0180004793.01.T01:CDS
TCAGGTTCAGGAGGTTCAGA+CGG	0.613881	1:-82786383	MsG0180004793.01.T01:CDS
TAGAGAATGACCGTCTCGCG+CGG	0.619664	1:-82786232	MsG0180004793.01.T01:CDS
CCTCAGTCTGGATCGTCTCG+CGG	0.620361	1:+82786304	None:intergenic
CCGCGAGACGATCCAGACTG+AGG	0.625229	1:-82786304	MsG0180004793.01.T01:CDS
CAACAGGTTGAAGATCTGAC+CGG	0.629630	1:-82786275	MsG0180004793.01.T01:CDS
GCGGAGTATTAAGGCGACGG+AGG	0.691194	1:-82786213	MsG0180004793.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	ACCTTATCCAAATTATTCTA+TGG	-	Chr1:82786390-82786409	MsG0180004793.01.T01:CDS	25.0%
!!	ATTCTCTATTTTCAATCTAC+CGG	+	Chr1:82786158-82786177	None:intergenic	25.0%
	GCCATAGAATAATTTGGATA+AGG	+	Chr1:82786394-82786413	None:intergenic	30.0%
	TGTGATGCCATAGAATAATT+TGG	+	Chr1:82786400-82786419	None:intergenic	30.0%
	AGAAGAGGATCATCAATCAA+AGG	+	Chr1:82786355-82786374	None:intergenic	35.0%
	TTTGGATAAGGTATGAACCA+AGG	+	Chr1:82786382-82786401	None:intergenic	35.0%
	AAGAATTGGAGGCTCAGTTT+CGG	-	Chr1:82786263-82786282	MsG0180004793.01.T01:CDS	40.0%
	ATGAACCAAGGTTTGAGAAG+AGG	+	Chr1:82786370-82786389	None:intergenic	40.0%
	ATGATCCTCTTCTCAAACCT+TGG	-	Chr1:82786362-82786381	MsG0180004793.01.T01:CDS	40.0%
	ATTCAACGTCCTGTAAGTTC+AGG	-	Chr1:82785995-82786014	MsG0180004793.01.T01:CDS	40.0%
	AGCGCAGACAAAAGAATTGG+AGG	-	Chr1:82786252-82786271	MsG0180004793.01.T01:CDS	45.0%
	CAGAGCGCAGACAAAAGAAT+TGG	-	Chr1:82786249-82786268	MsG0180004793.01.T01:CDS	45.0%
	GAACCTGAACCTGAACTTAC+AGG	+	Chr1:82786007-82786026	None:intergenic	45.0%
!	CAACAGGTTGAAGATCTGAC+CGG	-	Chr1:82786127-82786146	MsG0180004793.01.T01:CDS	45.0%
!	GTAAGTTCAGGTTCAGGTTC+AGG	-	Chr1:82786007-82786026	MsG0180004793.01.T01:CDS	45.0%
!!	TTTCAATCTACCGGCTTCTC+CGG	+	Chr1:82786149-82786168	None:intergenic	45.0%
	CAGACTGAGGAAGCAACAAC+AGG	-	Chr1:82786111-82786130	MsG0180004793.01.T01:CDS	50.0%
	TCAGGTTCAGGAGGTTCAGA+CGG	-	Chr1:82786019-82786038	MsG0180004793.01.T01:CDS	50.0%
	TTCAGGAGGTTCAGACGGTA+TGG	-	Chr1:82786024-82786043	MsG0180004793.01.T01:CDS	50.0%
!	AGTTCAGGTTCAGGTTCAGG+AGG	-	Chr1:82786010-82786029	MsG0180004793.01.T01:CDS	50.0%
!	CGTCCTGTAAGTTCAGGTTC+AGG	-	Chr1:82786001-82786020	MsG0180004793.01.T01:CDS	50.0%
!	CTGTTGTTGCTTCCTCAGTC+TGG	+	Chr1:82786113-82786132	None:intergenic	50.0%
!	GGAGGAAGCGTATGTGAAGA+TGG	-	Chr1:82786207-82786226	MsG0180004793.01.T01:CDS	50.0%
	AGATGCTGCTGCTGCTGAAG+AGG	-	Chr1:82786303-82786322	MsG0180004793.01.T01:CDS	55.0%
	CCTTAATACTCCGCGCGAGA+CGG	+	Chr1:82786183-82786202	None:intergenic	55.0%
	GAAGATCTGACCGGAGAAGC+CGG	-	Chr1:82786136-82786155	MsG0180004793.01.T01:CDS	55.0%
	TAGAGAATGACCGTCTCGCG+CGG	-	Chr1:82786170-82786189	MsG0180004793.01.T01:CDS	55.0%
	TGGATCGTCTCGCGGATTCA+CGG	+	Chr1:82786093-82786112	None:intergenic	55.0%
	CCTCAGTCTGGATCGTCTCG+CGG	+	Chr1:82786101-82786120	None:intergenic	60.0%
!	CCGTCTCGCGCGGAGTATTA+AGG	-	Chr1:82786180-82786199	MsG0180004793.01.T01:CDS	60.0%
!	CGCGCGGAGTATTAAGGCGA+CGG	-	Chr1:82786186-82786205	MsG0180004793.01.T01:CDS	60.0%
!	GCGGAGTATTAAGGCGACGG+AGG	-	Chr1:82786189-82786208	MsG0180004793.01.T01:CDS	60.0%
	CCGCGAGACGATCCAGACTG+AGG	-	Chr1:82786098-82786117	MsG0180004793.01.T01:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	82785986	82786438	82785986	ID=MsG0180004793.01;Name=MsG0180004793.01
Chr1	mRNA	82785986	82786438	82785986	ID=MsG0180004793.01.T01;Parent=MsG0180004793.01;Name=MsG0180004793.01.T01;_AED=0.37;_eAED=0.37;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|150
Chr1	exon	82785986	82786438	82785986	ID=MsG0180004793.01.T01:exon:8659;Parent=MsG0180004793.01.T01
Chr1	CDS	82785986	82786438	82785986	ID=MsG0180004793.01.T01:cds;Parent=MsG0180004793.01.T01

Gene Sequence

>MsG0180004793.01.T01

ATGGCTTCGATTCAACGTCCTGTAAGTTCAGGTTCAGGTTCAGGAGGTTCAGACGGTATGGATCTACAAATCGACGAACGAAAACGCAAGAGAATGCTGTCAAACCGTGAATCCGCGAGACGATCCAGACTGAGGAAGCAACAACAGGTTGAAGATCTGACCGGAGAAGCCGGTAGATTGAAAATAGAGAATGACCGTCTCGCGCGGAGTATTAAGGCGACGGAGGAAGCGTATGTGAAGATGGAAGCTGCTAATGATGTTATCAGAGCGCAGACAAAAGAATTGGAGGCTCAGTTTCGGTTTTTGAATTCTGTTATAGATGCTGCTGCTGCTGAAGAGGCTAATAGTTTCTCTGTCGATGATGTGCCTTTGATTGATGATCCTCTTCTCAAACCTTGGTTCATACCTTATCCAAATTATTCTATGGCATCACATGAAATGATGCTGCGTTGA

Protein sequence

>MsG0180004793.01.T01

MASIQRPVSSGSGSGGSDGMDLQIDERKRKRMLSNRESARRSRLRKQQQVEDLTGEAGRLKIENDRLARSIKATEEAYVKMEAANDVIRAQTKELEAQFRFLNSVIDAAAAEEANSFSVDDVPLIDDPLLKPWFIPYPNYSMASHEMMLR*