AlfalfaGEDB | MsG0480020964.01

M. sativa cultivar ZhongmuNo.1 / MsG0480020964.01

KEGG: -
GO: GO:0004672 GO:0005524 GO:0006468
PFAM: PF07714

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480020964.01.T01	XP_024637586.1	95.355	409	4	1	1	394	45	453	0	802

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480020964.01.T01	Q9SF86	62.136	309	101	2	29	322	65	372	3.09E-135	396

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480020964.01.T01	A0A396I8B4	95.355	409	4	1	1	394	45	453	0.0	802

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080047797.01	MsG0480020964.01	0.801480	8.827061e-49	4.219997e-46
MsG0080048178.01	MsG0480020964.01	0.825931	3.581865e-54	3.311106e-51
MsG0080048726.01	MsG0480020964.01	0.812365	4.397637e-51	2.790708e-48
MsG0180000116.01	MsG0480020964.01	0.816990	4.159595e-52	2.992477e-49
MsG0180000267.01	MsG0480020964.01	0.801080	1.065626e-48	5.043587e-46
MsG0180000307.01	MsG0480020964.01	0.800073	1.710396e-48	7.890511e-46
MsG0180000470.01	MsG0480020964.01	0.822281	2.576813e-53	2.147223e-50
MsG0180000555.01	MsG0480020964.01	0.800832	1.197772e-48	5.633188e-46
MsG0180000585.01	MsG0480020964.01	0.803986	2.683151e-49	1.367515e-46
MsG0180001122.01	MsG0480020964.01	0.806225	9.119514e-50	4.923317e-47

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PPI

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Gene1	Gene2	Type
MsG0280007018.01	MsG0480020964.01	PPI
MsG0480018334.01	MsG0480020964.01	PPI
MsG0780040233.01	MsG0480020964.01	PPI
MsG0880047337.01	MsG0480020964.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480020964.01.T01	MTR_4g063950	95.545	404	8	1	1	394	45	448	0.0	795
MsG0480020964.01.T01	MTR_1g063910	55.585	376	141	6	2	353	38	411	6.98e-141	408
MsG0480020964.01.T01	MTR_1g063910	55.585	376	141	6	2	353	47	420	1.03e-140	408
MsG0480020964.01.T01	MTR_7g101800	56.395	344	126	4	1	322	33	374	1.86e-137	399
MsG0480020964.01.T01	MTR_7g101800	56.395	344	126	4	1	322	41	382	3.01e-137	399
MsG0480020964.01.T01	MTR_7g101800	56.395	344	126	4	1	322	53	394	4.02e-137	399
MsG0480020964.01.T01	MTR_8g008820	55.689	334	125	3	25	343	53	378	6.33e-121	357
MsG0480020964.01.T01	MTR_4g099130	55.556	306	120	2	25	315	46	350	1.33e-115	342
MsG0480020964.01.T01	MTR_5g013610	54.072	307	122	4	25	315	41	344	2.35e-110	327
MsG0480020964.01.T01	MTR_8g469230	51.803	305	122	4	30	315	114	412	6.34e-99	303

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480020964.01.T01	AT2G28940	65.947	417	112	8	3	394	51	462	0.0	524
MsG0480020964.01.T01	AT2G28940	71.835	316	82	5	81	394	33	343	2.94e-160	454
MsG0480020964.01.T01	AT3G09830	62.136	309	101	2	29	322	65	372	3.15e-136	396
MsG0480020964.01.T01	AT3G09830	62.136	309	101	2	29	322	65	372	3.15e-136	396
MsG0480020964.01.T01	AT3G09830	62.136	309	101	2	29	322	65	372	3.15e-136	396
MsG0480020964.01.T01	AT5G03320	57.849	344	123	4	2	324	29	371	2.76e-133	389
MsG0480020964.01.T01	AT5G03320	57.849	344	123	4	2	324	29	371	2.76e-133	389
MsG0480020964.01.T01	AT2G39110	61.022	313	107	1	25	322	67	379	2.42e-129	379
MsG0480020964.01.T01	AT2G39110	61.022	313	107	1	25	322	52	364	3.35e-129	378
MsG0480020964.01.T01	AT5G47070	50.278	360	152	7	3	342	38	390	1.67e-106	320

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Find 93 sgRNAs with CRISPR-Local

Find 194 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACATAGCCAAAGCCTTCTG+CGG	0.598757	4:+51272199	None:intergenic
AACCTGTACCAGCTAAACAG+GGG	0.726480	4:-51271449	MsG0480020964.01.T01:CDS
AAGAGGGATTCAGAGGCTTT+TGG	0.356513	4:-51273514	MsG0480020964.01.T01:CDS
AAGAGTGATGTGTGGAGCTT+TGG	0.430091	4:-51271825	MsG0480020964.01.T01:CDS
AAGATAAGCCAACCCACGAG+CGG	0.671638	4:+51273389	None:intergenic
AAGATTTGATTGGAAGAGTT+GGG	0.443023	4:-51271361	MsG0480020964.01.T01:CDS
AATCCATTGGAAGATTTGAT+TGG	0.351673	4:-51271371	MsG0480020964.01.T01:CDS
AATCGGAATCGTATTCGGAG+CGG	0.728176	4:+51273976	None:intergenic
AATGCAAAGCTTTCTGATTT+TGG	0.064179	4:-51272239	MsG0480020964.01.T01:CDS
AATGCATTCATCCATAGGAA+CGG	0.648614	4:+51271533	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAAAAACCAAACCAATTGC+TGG	+	Chr4:51272030-51272049	None:intergenic	30.0%
AAAAAACGAACAAAAGCTTC+TGG	-	Chr4:51273574-51273593	MsG0480020964.01.T01:CDS	30.0%
AAGATTTGATTGGAAGAGTT+GGG	-	Chr4:51273961-51273980	MsG0480020964.01.T01:CDS	30.0%
AATCCATTGGAAGATTTGAT+TGG	-	Chr4:51273951-51273970	MsG0480020964.01.T01:CDS	30.0%
GTGATGATTATGATGAACTA+GGG	+	Chr4:51271534-51271553	None:intergenic	30.0%
GTTTATGTATGGTTTATGCA+TGG	-	Chr4:51272959-51272978	MsG0480020964.01.T01:intron	30.0%
TACTAGATTAAGAATTGCTC+AGG	-	Chr4:51271907-51271926	MsG0480020964.01.T01:intron	30.0%
TCTTTCATGAAAACTTCGAA+AGG	+	Chr4:51272485-51272504	None:intergenic	30.0%
TTCTTTGGATCAGATACATA+CGG	+	Chr4:51273611-51273630	None:intergenic	30.0%
AAGAAGAGTAGATCAAATGG+AGG	+	Chr4:51272360-51272379	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	51271335	51274009	51271335	ID=MsG0480020964.01;Name=MsG0480020964.01
Chr4	mRNA	51271335	51274009	51271335	ID=MsG0480020964.01.T01;Parent=MsG0480020964.01;Name=MsG0480020964.01.T01;_AED=0.49;_eAED=0.50;_QI=0\|0\|0\|1\|1\|1\|4\|0\|394
Chr4	exon	51273748	51274009	51273748	ID=MsG0480020964.01.T01:exon:439;Parent=MsG0480020964.01.T01
Chr4	exon	51273368	51273594	51273368	ID=MsG0480020964.01.T01:exon:438;Parent=MsG0480020964.01.T01
Chr4	exon	51272190	51272312	51272190	ID=MsG0480020964.01.T01:exon:437;Parent=MsG0480020964.01.T01
Chr4	exon	51271335	51271907	51271335	ID=MsG0480020964.01.T01:exon:436;Parent=MsG0480020964.01.T01
Chr4	CDS	51273748	51274009	51273748	ID=MsG0480020964.01.T01:cds;Parent=MsG0480020964.01.T01
Chr4	CDS	51273368	51273594	51273368	ID=MsG0480020964.01.T01:cds;Parent=MsG0480020964.01.T01
Chr4	CDS	51272190	51272312	51272190	ID=MsG0480020964.01.T01:cds;Parent=MsG0480020964.01.T01
Chr4	CDS	51271335	51271907	51271335	ID=MsG0480020964.01.T01:cds;Parent=MsG0480020964.01.T01

Gene Sequence

>MsG0480020964.01.T01

ATGGACACGCGCCGCTCCGAATACGATTCCGATTCTAGAGACTTCTCCGACACGCTTGGCTTCCATGAGTTTCTCTCTCAACGCCGTGCTAATCATCTTCATCTGTTTTCATTCTCTGACCTCAAAATCGCTACTCGTGGATTCAATAGAGCGTTGTTAATTGGTGAAGGTGGATTTGGTTCGGTTTATAGAGGAACCCTAGTTCATCATAATCATCACCGTTCTCAAATCGATGTTGCCATCAAGCAGTTGAATCGTAATGGGGTGATTAAGCATCCAAATTTGGTGAGGTTAGTTGGTTATTGTGCTGAAGATGATGAAAGAGGGATTCAGAGGCTTTTGGTGTATGAGTTTATGTCTAATAAGAGTTTGGAAGATCATCTTTTGGTTCGTGTTCCTTCGTCGGTTCTTCCTTGGATTACTAGATTAAGAATTGCTCAGGATGCCGCTCGTGGGTTGGCTTATCTTCACGAGGAAATGGATTTTCAGCTAATATTTCGAGATTTTAAAACATCCAACATCTTGCTGGATGAGGACTTCAATGCAAAGCTTTCTGATTTTGGACTCGCTAGGCAAGGACCCGCAGAAGGCTTTGGCTATGTTTCTACAGCGGTTGTTGGCACCATAGGATATGCTGCACCAGAGTATGTTCAAACCGGAAAGCTGACTGCTAAGAGTGATGTGTGGAGCTTTGGTGTAGTTCTGTATGAGCTTATCACTGGGAGGAGAGCTGTAGAAAGAAACCTACCAAAAAACGAACAAAAGCTTCTGGATTGGGTTAGACCGTATGTATCTGATCCAAAGAAGTTCCATCATATTGTCGATCCTCGACTTGAGGCACAGGATTGCACCAAATCAGCTCATAAACTTGCAGTCCTGGCAAACAAGTGTCTCATGAAGCAGCCAAAGTCTCGCCCAAAAATGAGCGAGGTGGTTGAGATTCTTGGGAACATTATTGACGAGTCCGTTCCTATGGATGAATGCATTTCCGAAGCTGTTGCAGAAACAGGAGAAGCAAATGAAGTAAATTTATCTGTAGAGAATGCTGAACCTGTACCAGCTAAACAGGGGAATAATTATCTGAAGAAGGTTTTTGAATTCAGAGACAAGGTGAGTTTAAGAAATAAATCCATTGGAAGATTTGATTGGAAGAGTTGGGCTCCTGGGCTTGTAAGAACATGGTGA

Protein sequence

>MsG0480020964.01.T01

MDTRRSEYDSDSRDFSDTLGFHEFLSQRRANHLHLFSFSDLKIATRGFNRALLIGEGGFGSVYRGTLVHHNHHRSQIDVAIKQLNRNGVIKHPNLVRLVGYCAEDDERGIQRLLVYEFMSNKSLEDHLLVRVPSSVLPWITRLRIAQDAARGLAYLHEEMDFQLIFRDFKTSNILLDEDFNAKLSDFGLARQGPAEGFGYVSTAVVGTIGYAAPEYVQTGKLTAKSDVWSFGVVLYELITGRRAVERNLPKNEQKLLDWVRPYVSDPKKFHHIVDPRLEAQDCTKSAHKLAVLANKCLMKQPKSRPKMSEVVEILGNIIDESVPMDECISEAVAETGEANEVNLSVENAEPVPAKQGNNYLKKVFEFRDKVSLRNKSIGRFDWKSWAPGLVRTW*