AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280011241.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280011241.01.T01 MTR_2g100500 93.452 336 16 1 1 330 1 336 0.0 643
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280011241.01.T01 AT3G21300 66.667 279 87 2 56 330 54 330 3.68e-139 405

Find 105 sgRNAs with CRISPR-Local

Find 208 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTGTTAGGGTTTGGGGTTT+TGG 0.151935 2:+81377938 None:intergenic
AATTGGAGGTGTTAGGGTTT+GGG 0.184627 2:+81377931 None:intergenic
GTTGGTTCTGGTTGTGGTTC+GGG 0.205534 2:+81377978 None:intergenic
TCTTTAGGCAACCATTTATA+TGG 0.207383 2:+81376626 None:intergenic
TCTCATTTGGTCCATATAAA+TGG 0.212001 2:-81376637 MsG0280011241.01.T01:CDS
TCGAGGGTTTGGCCGCGTTT+AGG 0.242935 2:+81377900 None:intergenic
ACAAACTGATCCTGCTAATA+AGG 0.268322 2:-81376498 MsG0280011241.01.T01:intron
GAAGTTGTGGTGGTTGGTTC+TGG 0.271287 2:+81377966 None:intergenic
CTTCCCGGTGAGCGTTTCAT+TGG 0.287130 2:-81377792 MsG0280011241.01.T01:CDS
GACGCAGAATTTGGCTTATC+AGG 0.288607 2:-81377652 MsG0280011241.01.T01:CDS
GCACTTGGATTGCATGTTCC+TGG 0.301094 2:-81376560 MsG0280011241.01.T01:CDS
GGTTGGTTCTGGTTGTGGTT+CGG 0.306125 2:+81377977 None:intergenic
CGAGGGTTTGGCCGCGTTTA+GGG 0.308019 2:+81377901 None:intergenic
CCTTGTTTCTATAATGAAAC+TGG 0.314926 2:+81376772 None:intergenic
TTGGCCGCGTTTAGGGAAAT+AGG 0.315601 2:+81377908 None:intergenic
GAATTGGAGGTGTTAGGGTT+TGG 0.315723 2:+81377930 None:intergenic
CTTTCGTGCAGCGACTCTTT+AGG 0.323603 2:+81376611 None:intergenic
CGCAGAGTAGTTCGAGGGTT+TGG 0.329817 2:+81377889 None:intergenic
AATAGGAATTGGAGGTGTTA+GGG 0.340379 2:+81377925 None:intergenic
AAATAGGAATTGGAGGTGTT+AGG 0.347221 2:+81377924 None:intergenic
GTGTAGATGGAATTCTCATT+TGG 0.359119 2:-81376650 MsG0280011241.01.T01:intron
GGAGTGGGAGTGTGTTTGTT+AGG 0.369822 2:+81378072 None:intergenic
TGGTTGTAAGACGCAGAATT+TGG 0.377540 2:-81377661 MsG0280011241.01.T01:CDS
ATTGGAGGTGTTAGGGTTTG+GGG 0.387688 2:+81377932 None:intergenic
TACTAGGACCTTCAGCAAAT+CGG 0.389943 2:+81375446 None:intergenic
TTGAAGAAGCAAGAAGCATT+TGG 0.396766 2:-81378132 MsG0280011241.01.T01:five_prime_UTR
ACTTAAGTACCAACCACTTC+GGG 0.397718 2:+81375405 None:intergenic
CTTCAGCAAATCGGGCTTGT+AGG 0.401351 2:+81375455 None:intergenic
GCGTTTAGGGAAATAGGAAT+TGG 0.426518 2:+81377914 None:intergenic
ATGTGTGACCGTGCACTTCC+CGG 0.431326 2:-81377807 MsG0280011241.01.T01:CDS
CAGAAAGACTTGGAATTGAA+TGG 0.432456 2:-81376825 MsG0280011241.01.T01:CDS
AGCAAAACGGTGAGAGGGAG+AGG 0.432701 2:+81378025 None:intergenic
GATTAAAGCTAAAGAGGAAC+AGG 0.438005 2:-81377625 MsG0280011241.01.T01:intron
TTCTGGTTGTGGTTCGGGGA+GGG 0.444485 2:+81377983 None:intergenic
AGAAGCTGCACTGTAGAGTA+AGG 0.448355 2:+81378157 None:intergenic
TTCCCGGTGAGCGTTTCATT+GGG 0.451248 2:-81377791 MsG0280011241.01.T01:CDS
ACTAGGACCTTCAGCAAATC+GGG 0.455837 2:+81375447 None:intergenic
GTCACACATGACGACAAAAC+CGG 0.469567 2:+81377821 None:intergenic
CTATGCAAAGTTACTGATAC+CGG 0.471685 2:-81377840 MsG0280011241.01.T01:CDS
TTTGTGTGAATGAACATTGT+AGG 0.475932 2:+81376287 None:intergenic
TGTTCTAGGGATGTAATGAC+TGG 0.481380 2:-81375513 MsG0280011241.01.T01:intron
GTGGTGGTTGGTTCTGGTTG+TGG 0.481772 2:+81377972 None:intergenic
GGCGCATCAACAACATCAAA+AGG 0.484191 2:+81377708 None:intergenic
TGGGAGGATTACTCGGAAGA+AGG 0.484281 2:-81377772 MsG0280011241.01.T01:CDS
TGGATATCACAAGGAACAAT+CGG 0.487695 2:+81376792 None:intergenic
GAATTATGCGGAAGCTACTA+AGG 0.488030 2:-81377748 MsG0280011241.01.T01:CDS
CACGAAAGAAATGTTGATGC+TGG 0.489316 2:-81376596 MsG0280011241.01.T01:CDS
GGAATTGAAGTTGTGGTGGT+TGG 0.489461 2:+81377960 None:intergenic
GGCTCAGATTAAAGCTAAAG+AGG 0.496629 2:-81377631 MsG0280011241.01.T01:CDS
ATGCAGAAACTTTATCTACT+AGG 0.496736 2:+81375430 None:intergenic
CTCCCAATGAAACGCTCACC+GGG 0.501880 2:+81377789 None:intergenic
GGGAGGATTACTCGGAAGAA+GGG 0.502279 2:-81377771 MsG0280011241.01.T01:CDS
AAACGGTGAGAGGGAGAGGA+TGG 0.504252 2:+81378029 None:intergenic
ACATTGCATACCTTATTAGC+AGG 0.505849 2:+81376488 None:intergenic
ACCTTTGCCTTTAAAAGCGA+GGG 0.514290 2:+81377863 None:intergenic
TTTGGGAATTGAAGTTGTGG+TGG 0.514781 2:+81377956 None:intergenic
CCGGTGAGCGTTTCATTGGG+AGG 0.523671 2:-81377788 MsG0280011241.01.T01:CDS
TTGCGGCTATACAAGAATGT+TGG 0.526698 2:-81376335 MsG0280011241.01.T01:CDS
GACCTTTGCCTTTAAAAGCG+AGG 0.526847 2:+81377862 None:intergenic
GTTTCATTGGGAGGATTACT+CGG 0.528359 2:-81377779 MsG0280011241.01.T01:CDS
CCAGTTTCATTATAGAAACA+AGG 0.528382 2:-81376772 MsG0280011241.01.T01:intron
TGTTGGAGAGACCCACAACT+TGG 0.532486 2:-81376318 MsG0280011241.01.T01:CDS
AGTTTCTGCATTTCCCGAAG+TGG 0.533202 2:-81375418 MsG0280011241.01.T01:CDS
AACTTAAGTACCAACCACTT+CGG 0.533340 2:+81375404 None:intergenic
GGGAGTGAAAATTATGCACT+TGG 0.538441 2:-81376575 MsG0280011241.01.T01:CDS
TTGGTTCTGGTTGTGGTTCG+GGG 0.541358 2:+81377979 None:intergenic
ATATAAACAGGTCCGTGACT+TGG 0.545107 2:-81376871 MsG0280011241.01.T01:intron
ACAAAGTTGACCATTACTTC+AGG 0.548753 2:+81375486 None:intergenic
GTTCTGGTTGTGGTTCGGGG+AGG 0.555312 2:+81377982 None:intergenic
CTACAAGCCCGATTTGCTGA+AGG 0.558205 2:-81375454 MsG0280011241.01.T01:CDS
GACTGGTCTGCCTGAAGTAA+TGG 0.558686 2:-81375496 MsG0280011241.01.T01:CDS
AGGGTGAGAAACCAAGTTGT+GGG 0.558865 2:+81376307 None:intergenic
AGAGAGAGCAAAACGGTGAG+AGG 0.560069 2:+81378019 None:intergenic
GAGAGAGCAAAACGGTGAGA+GGG 0.560392 2:+81378020 None:intergenic
TTGTGTGAATGAACATTGTA+GGG 0.564703 2:+81376288 None:intergenic
CCTCCCAATGAAACGCTCAC+CGG 0.567152 2:+81377788 None:intergenic
TTTAGGGAAATAGGAATTGG+AGG 0.569455 2:+81377917 None:intergenic
AAGCATTTGATGCTGAGAAG+TGG 0.572814 2:-81376255 MsG0280011241.01.T01:CDS
CAAATCGGGCTTGTAGGAAG+AGG 0.574944 2:+81375461 None:intergenic
ACTTGGTCATACATGTTGGA+AGG 0.576915 2:-81376854 MsG0280011241.01.T01:CDS
TCTGCATTTCCCGAAGTGGT+TGG 0.579784 2:-81375414 MsG0280011241.01.T01:CDS
AAGCTGCACTGTAGAGTAAG+GGG 0.580198 2:+81378159 None:intergenic
AACGGTGAGAGGGAGAGGAT+GGG 0.581626 2:+81378030 None:intergenic
GAAGCTGCACTGTAGAGTAA+GGG 0.586153 2:+81378158 None:intergenic
CGTGACTTGGTCATACATGT+TGG 0.590483 2:-81376858 MsG0280011241.01.T01:CDS
TAGGGTGAGAAACCAAGTTG+TGG 0.599583 2:+81376306 None:intergenic
GGGATTCGCAGAGTAGTTCG+AGG 0.603852 2:+81377883 None:intergenic
AGAGAGTAGAGAGAGCAAAA+CGG 0.604713 2:+81378012 None:intergenic
AACGCTCACCGGGAAGTGCA+CGG 0.608647 2:+81377799 None:intergenic
GGAGGATTACTCGGAAGAAG+GGG 0.610224 2:-81377770 MsG0280011241.01.T01:CDS
AACATCTGAACAGGTTCTTG+CGG 0.610504 2:-81376352 MsG0280011241.01.T01:intron
CAATGCGGAACATAAACACA+AGG 0.621712 2:+81377687 None:intergenic
TACAATGTTCATTCACACAA+AGG 0.623279 2:-81376285 MsG0280011241.01.T01:CDS
GGATTCGCAGAGTAGTTCGA+GGG 0.629285 2:+81377884 None:intergenic
GTTTATGTTCCGCATTGTGG+TGG 0.642868 2:-81377681 MsG0280011241.01.T01:CDS
TGTGTTTATGTTCCGCATTG+TGG 0.644470 2:-81377684 MsG0280011241.01.T01:CDS
ACGAAAGAAATGTTGATGCT+GGG 0.651196 2:-81376595 MsG0280011241.01.T01:CDS
ATTTGATGCTGAGAAGTGGA+AGG 0.658726 2:-81376251 MsG0280011241.01.T01:intron
TAATGAAACTGGATATCACA+AGG 0.661138 2:+81376783 None:intergenic
AATTCCTATTTCCCTAAACG+CGG 0.665097 2:-81377912 MsG0280011241.01.T01:CDS
TCGGAAGAAGGGGAATTATG+CGG 0.683201 2:-81377760 MsG0280011241.01.T01:CDS
GCATCAACAACATCAAAAGG+AGG 0.683556 2:+81377711 None:intergenic
AGCTGCACTGTAGAGTAAGG+GGG 0.692085 2:+81378160 None:intergenic
CAACATGTATGACCAAGTCA+CGG 0.695459 2:+81376859 None:intergenic
GTCTTACAACCACCACAATG+CGG 0.732370 2:+81377672 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATATTTAATTTTTGTTCTA+GGG - Chr2:81378060-81378079 MsG0280011241.01.T01:CDS 10.0%
!!! TAATATTTAATTTTTGTTCT+AGG - Chr2:81378059-81378078 MsG0280011241.01.T01:CDS 10.0%
!! ATAACAATATTTCATTTGAA+GGG - Chr2:81378015-81378034 MsG0280011241.01.T01:CDS 15.0%
!! ATTAAACTGTTTATATAAAC+AGG - Chr2:81376703-81376722 MsG0280011241.01.T01:intron 15.0%
!! TTAAATAATTTATCCAAACA+AGG - Chr2:81376544-81376563 MsG0280011241.01.T01:CDS 15.0%
!!! ATAATTTTATCAAAAAAACC+AGG + Chr2:81377047-81377066 None:intergenic 15.0%
!! AAATACTGCTTATAAGTAAT+CGG + Chr2:81377504-81377523 None:intergenic 20.0%
!! ATATGAAATCAAATAAGTGT+AGG + Chr2:81377170-81377189 None:intergenic 20.0%
!! ATTAATACTGATAGAAAACA+AGG + Chr2:81378043-81378062 None:intergenic 20.0%
!! CATAACAATATTTCATTTGA+AGG - Chr2:81378014-81378033 MsG0280011241.01.T01:CDS 20.0%
!! CATGGAAAACTTATATAAAT+AGG + Chr2:81376570-81376589 None:intergenic 20.0%
!! GTTTATTTGAGCTTATTTAT+TGG - Chr2:81376015-81376034 MsG0280011241.01.T01:intron 20.0%
!!! ACATTGATTTTAAATTGGTA+TGG - Chr2:81377800-81377819 MsG0280011241.01.T01:CDS 20.0%
!!! CTCAAACATTGATTTTAAAT+TGG - Chr2:81377795-81377814 MsG0280011241.01.T01:CDS 20.0%
! AGAGTCTATCATATATATGT+TGG + Chr2:81377630-81377649 None:intergenic 25.0%
! CAACTTATGATTCATACATA+AGG + Chr2:81376161-81376180 None:intergenic 25.0%
! GAGTCTATCATATATATGTT+GGG + Chr2:81377629-81377648 None:intergenic 25.0%
! GAGTTTATAAAATCAGCTTA+TGG - Chr2:81376287-81376306 MsG0280011241.01.T01:CDS 25.0%
! TTGATTTCATATTCACAACT+GGG - Chr2:81377178-81377197 MsG0280011241.01.T01:intron 25.0%
!! CATATTTTATGAAGTTTCGT+TGG + Chr2:81377947-81377966 None:intergenic 25.0%
!! TTTGATTTCATATTCACAAC+TGG - Chr2:81377177-81377196 MsG0280011241.01.T01:intron 25.0%
!!! AAAAAGCATCAAATTCAAAG+GGG - Chr2:81377988-81378007 MsG0280011241.01.T01:CDS 25.0%
!!! GAAAAAGCATCAAATTCAAA+GGG - Chr2:81377987-81378006 MsG0280011241.01.T01:CDS 25.0%
!!! GTATGGAGTTTATTTTAACT+CGG - Chr2:81377817-81377836 MsG0280011241.01.T01:CDS 25.0%
!!! GTGGTTTTGTAAGTTTATTT+AGG + Chr2:81377463-81377482 None:intergenic 25.0%
!!! TATGGAGTTTATTTTAACTC+GGG - Chr2:81377818-81377837 MsG0280011241.01.T01:CDS 25.0%
!!! TGAAAAAGCATCAAATTCAA+AGG - Chr2:81377986-81378005 MsG0280011241.01.T01:CDS 25.0%
!!! TTATATAAATAGGCCTTGTT+TGG + Chr2:81376560-81376579 None:intergenic 25.0%
!!! TTTGAATTTGATGCTTTTTC+AGG + Chr2:81377987-81378006 None:intergenic 25.0%
ACTTACAAAACCACCTTATA+AGG - Chr2:81377469-81377488 MsG0280011241.01.T01:intron 30.0%
ATGCAGAAACTTTATCTACT+AGG + Chr2:81378159-81378178 None:intergenic 30.0%
CCAGTTTCATTATAGAAACA+AGG - Chr2:81376814-81376833 MsG0280011241.01.T01:CDS 30.0%
GATGTTCAAGACAAAACATA+AGG + Chr2:81377220-81377239 None:intergenic 30.0%
GTTTGAATAGTGATAGTAAG+TGG + Chr2:81377734-81377753 None:intergenic 30.0%
TAATGAAACTGGATATCACA+AGG + Chr2:81376806-81376825 None:intergenic 30.0%
TACAATGTTCATTCACACAA+AGG - Chr2:81377301-81377320 MsG0280011241.01.T01:intron 30.0%
TATGAAGTTTCGTTGGAATT+TGG + Chr2:81377940-81377959 None:intergenic 30.0%
TCTCATTTGGTCCATATAAA+TGG - Chr2:81376949-81376968 MsG0280011241.01.T01:intron 30.0%
TCTTTAGGCAACCATTTATA+TGG + Chr2:81376963-81376982 None:intergenic 30.0%
TGTTTACTTTCTTGTGTAGA+TGG - Chr2:81376923-81376942 MsG0280011241.01.T01:intron 30.0%
TTGTGTGAATGAACATTGTA+GGG + Chr2:81377301-81377320 None:intergenic 30.0%
TTTGTGTGAATGAACATTGT+AGG + Chr2:81377302-81377321 None:intergenic 30.0%
! AGGATGGGAATTTGAATTTT+CGG + Chr2:81375544-81375563 None:intergenic 30.0%
! GGAATTTGAATTTTCGGAAA+TGG + Chr2:81375538-81375557 None:intergenic 30.0%
!! CCTTGTTTCTATAATGAAAC+TGG + Chr2:81376817-81376836 None:intergenic 30.0%
!! TTCCTTGTCTTACTTGTTTT+GGG - Chr2:81377378-81377397 MsG0280011241.01.T01:intron 30.0%
!!! AATTGTTCTTGTCATTTTCG+CGG + Chr2:81375489-81375508 None:intergenic 30.0%
!!! GTTTATTTTAACTCGGGTAA+AGG - Chr2:81377824-81377843 MsG0280011241.01.T01:CDS 30.0%
AACCATATCACTATGCAATG+TGG - Chr2:81377751-81377770 MsG0280011241.01.T01:CDS 35.0%
AACTACAACTTGTGCTATCA+TGG + Chr2:81376588-81376607 None:intergenic 35.0%
AAGCCATCTTGGAAAACTTA+AGG + Chr2:81376413-81376432 None:intergenic 35.0%
AATTCCTATTTCCCTAAACG+CGG - Chr2:81375674-81375693 MsG0280011241.01.T01:intron 35.0%
ACAAAACCACCTTATAAGGT+GGG - Chr2:81377473-81377492 MsG0280011241.01.T01:intron 35.0%
ACAAACTGATCCTGCTAATA+AGG - Chr2:81377088-81377107 MsG0280011241.01.T01:intron 35.0%
ACAAAGTTGACCATTACTTC+AGG + Chr2:81378103-81378122 None:intergenic 35.0%
ACATTGCATACCTTATTAGC+AGG + Chr2:81377101-81377120 None:intergenic 35.0%
ACCATATCACTATGCAATGT+GGG - Chr2:81377752-81377771 MsG0280011241.01.T01:CDS 35.0%
ACGAAAGAAATGTTGATGCT+GGG - Chr2:81376991-81377010 MsG0280011241.01.T01:intron 35.0%
AGTTCTTGCATCTATATTCG+TGG - Chr2:81377417-81377436 MsG0280011241.01.T01:intron 35.0%
ATTCGTGGACCATAGAATTT+GGG - Chr2:81377432-81377451 MsG0280011241.01.T01:intron 35.0%
ATTTCATATTCACAACTGGG+AGG - Chr2:81377181-81377200 MsG0280011241.01.T01:intron 35.0%
CACTTTATAGTTCGTGACAT+AGG - Chr2:81376200-81376219 MsG0280011241.01.T01:intron 35.0%
CAGAAAGACTTGGAATTGAA+TGG - Chr2:81376761-81376780 MsG0280011241.01.T01:intron 35.0%
CTATGCAAAGTTACTGATAC+CGG - Chr2:81375746-81375765 MsG0280011241.01.T01:intron 35.0%
GAATAGTGATAGTAAGTGGT+GGG + Chr2:81377730-81377749 None:intergenic 35.0%
GATTAAAGCTAAAGAGGAAC+AGG - Chr2:81375961-81375980 MsG0280011241.01.T01:intron 35.0%
GTGTAGATGGAATTCTCATT+TGG - Chr2:81376936-81376955 MsG0280011241.01.T01:intron 35.0%
TAAGTGTTTGTGACACTGTT+TGG - Chr2:81376348-81376367 MsG0280011241.01.T01:intron 35.0%
TACAAAACCACCTTATAAGG+TGG - Chr2:81377472-81377491 MsG0280011241.01.T01:intron 35.0%
TACCCAAAACAAGTAAGACA+AGG + Chr2:81377383-81377402 None:intergenic 35.0%
TATCTGCATAAGCACTTCTA+AGG - Chr2:81376254-81376273 MsG0280011241.01.T01:CDS 35.0%
TATTCGTGGACCATAGAATT+TGG - Chr2:81377431-81377450 MsG0280011241.01.T01:intron 35.0%
TGAATAGTGATAGTAAGTGG+TGG + Chr2:81377731-81377750 None:intergenic 35.0%
TGGATATCACAAGGAACAAT+CGG + Chr2:81376797-81376816 None:intergenic 35.0%
TTTAGGGAAATAGGAATTGG+AGG + Chr2:81375672-81375691 None:intergenic 35.0%
TTTGTCTTGAACATCTGAAC+AGG - Chr2:81377225-81377244 MsG0280011241.01.T01:intron 35.0%
! AAGGTTTTCTCAGAAAGACT+TGG - Chr2:81376751-81376770 MsG0280011241.01.T01:intron 35.0%
! ATCAATGTTTGAGCCTTATC+CGG + Chr2:81377788-81377807 None:intergenic 35.0%
! GTTCCTTGTCTTACTTGTTT+TGG - Chr2:81377377-81377396 MsG0280011241.01.T01:intron 35.0%
! TCTCATGCAAATGCATCTTT+TGG - Chr2:81376875-81376894 MsG0280011241.01.T01:intron 35.0%
! TGAATTTTCGGAAATGGAGT+GGG + Chr2:81375532-81375551 None:intergenic 35.0%
! TTGAATTTTCGGAAATGGAG+TGG + Chr2:81375533-81375552 None:intergenic 35.0%
!! AAATAGGAATTGGAGGTGTT+AGG + Chr2:81375665-81375684 None:intergenic 35.0%
!! AATAGGAATTGGAGGTGTTA+GGG + Chr2:81375664-81375683 None:intergenic 35.0%
!! GCTGTTTTGAGCTTATCTAA+TGG - Chr2:81376324-81376343 MsG0280011241.01.T01:CDS 35.0%
!! GTTGTTTTCATAAGCCATCT+TGG + Chr2:81376424-81376443 None:intergenic 35.0%
!! TTGAAGAAGCAAGAAGCATT+TGG - Chr2:81375454-81375473 MsG0280011241.01.T01:CDS 35.0%
!!! TTACTTGTTTTGGGTACACA+TGG - Chr2:81377387-81377406 MsG0280011241.01.T01:intron 35.0%
AACATCTGAACAGGTTCTTG+CGG - Chr2:81377234-81377253 MsG0280011241.01.T01:intron 40.0%
AAGCATTTGATGCTGAGAAG+TGG - Chr2:81377331-81377350 MsG0280011241.01.T01:intron 40.0%
ACTTGGTCATACATGTTGGA+AGG - Chr2:81376732-81376751 MsG0280011241.01.T01:intron 40.0%
AGAGAGTAGAGAGAGCAAAA+CGG + Chr2:81375577-81375596 None:intergenic 40.0%
ATATAAACAGGTCCGTGACT+TGG - Chr2:81376715-81376734 MsG0280011241.01.T01:intron 40.0%
ATTTGATGCTGAGAAGTGGA+AGG - Chr2:81377335-81377354 MsG0280011241.01.T01:intron 40.0%
CAACATGTATGACCAAGTCA+CGG + Chr2:81376730-81376749 None:intergenic 40.0%
CAATGCGGAACATAAACACA+AGG + Chr2:81375902-81375921 None:intergenic 40.0%
CACGAAAGAAATGTTGATGC+TGG - Chr2:81376990-81377009 MsG0280011241.01.T01:intron 40.0%
CCATATCACTATGCAATGTG+GGG - Chr2:81377753-81377772 MsG0280011241.01.T01:CDS 40.0%
GAATTATGCGGAAGCTACTA+AGG - Chr2:81375838-81375857 MsG0280011241.01.T01:intron 40.0%
GCATCAACAACATCAAAAGG+AGG + Chr2:81375878-81375897 None:intergenic 40.0%
GCGTTTAGGGAAATAGGAAT+TGG + Chr2:81375675-81375694 None:intergenic 40.0%
GGCTCAGATTAAAGCTAAAG+AGG - Chr2:81375955-81375974 MsG0280011241.01.T01:intron 40.0%
GGGAGTGAAAATTATGCACT+TGG - Chr2:81377011-81377030 MsG0280011241.01.T01:intron 40.0%
GTTTCATTGGGAGGATTACT+CGG - Chr2:81375807-81375826 MsG0280011241.01.T01:intron 40.0%
TACTAGGACCTTCAGCAAAT+CGG + Chr2:81378143-81378162 None:intergenic 40.0%
TCGCACATTGGATAGTGATA+TGG + Chr2:81377530-81377549 None:intergenic 40.0%
TGACCTAGGTGACTCAATTA+CGG - Chr2:81377599-81377618 MsG0280011241.01.T01:intron 40.0%
TGGTTGTAAGACGCAGAATT+TGG - Chr2:81375925-81375944 MsG0280011241.01.T01:intron 40.0%
TGTGTTTATGTTCCGCATTG+TGG - Chr2:81375902-81375921 MsG0280011241.01.T01:intron 40.0%
TTGCGGCTATACAAGAATGT+TGG - Chr2:81377251-81377270 MsG0280011241.01.T01:intron 40.0%
TTTGGGAATTGAAGTTGTGG+TGG + Chr2:81375633-81375652 None:intergenic 40.0%
! AAAGCTGGCTTATATGGTGA+TGG - Chr2:81377701-81377720 MsG0280011241.01.T01:CDS 40.0%
! ACCTTTGCCTTTAAAAGCGA+GGG + Chr2:81375726-81375745 None:intergenic 40.0%
! GCACCTTAAGTTTTCCAAGA+TGG - Chr2:81376407-81376426 MsG0280011241.01.T01:intron 40.0%
! TATAGCATAAGAGCGCATCA+TGG + Chr2:81376515-81376534 None:intergenic 40.0%
! TTTAACTCGGGTAAAGGTGT+GGG - Chr2:81377830-81377849 MsG0280011241.01.T01:CDS 40.0%
!! AATTGGAGGTGTTAGGGTTT+GGG + Chr2:81375658-81375677 None:intergenic 40.0%
!! TGTTCTAGGGATGTAATGAC+TGG - Chr2:81378073-81378092 MsG0280011241.01.T01:CDS 40.0%
!! TTTTAACTCGGGTAAAGGTG+TGG - Chr2:81377829-81377848 MsG0280011241.01.T01:CDS 40.0%
!!! GGTTTTGGGAATTGAAGTTG+TGG + Chr2:81375636-81375655 None:intergenic 40.0%
AAGCTGCACTGTAGAGTAAG+GGG + Chr2:81375430-81375449 None:intergenic 45.0%
ACTAGGACCTTCAGCAAATC+GGG + Chr2:81378142-81378161 None:intergenic 45.0%
AGAAGCTGCACTGTAGAGTA+AGG + Chr2:81375432-81375451 None:intergenic 45.0%
AGGGTGAGAAACCAAGTTGT+GGG + Chr2:81377282-81377301 None:intergenic 45.0%
AGTTTCTGCATTTCCCGAAG+TGG - Chr2:81378168-81378187 MsG0280011241.01.T01:five_prime_UTR 45.0%
CCCCACATTGCATAGTGATA+TGG + Chr2:81377756-81377775 None:intergenic 45.0%
GAAGCTGCACTGTAGAGTAA+GGG + Chr2:81375431-81375450 None:intergenic 45.0%
GACCTAGGTGACTCAATTAC+GGG - Chr2:81377600-81377619 MsG0280011241.01.T01:intron 45.0%
GCATTTCCCACCTTATAAGG+TGG + Chr2:81377482-81377501 None:intergenic 45.0%
GGAATTGAAGTTGTGGTGGT+TGG + Chr2:81375629-81375648 None:intergenic 45.0%
GGCGCATCAACAACATCAAA+AGG + Chr2:81375881-81375900 None:intergenic 45.0%
GTCACACATGACGACAAAAC+CGG + Chr2:81375768-81375787 None:intergenic 45.0%
GTCTTACAACCACCACAATG+CGG + Chr2:81375917-81375936 None:intergenic 45.0%
GTGTTTGTGACACTGTTTGG+AGG - Chr2:81376351-81376370 MsG0280011241.01.T01:intron 45.0%
GTTTATGTTCCGCATTGTGG+TGG - Chr2:81375905-81375924 MsG0280011241.01.T01:intron 45.0%
TAGGCTCGACCCAAATTCTA+TGG + Chr2:81377444-81377463 None:intergenic 45.0%
TAGGGTGAGAAACCAAGTTG+TGG + Chr2:81377283-81377302 None:intergenic 45.0%
TCGGAAGAAGGGGAATTATG+CGG - Chr2:81375826-81375845 MsG0280011241.01.T01:intron 45.0%
TCGGCATTTCCCACCTTATA+AGG + Chr2:81377485-81377504 None:intergenic 45.0%
TTCGTGACATAGGACATGCT+TGG - Chr2:81376210-81376229 MsG0280011241.01.T01:intron 45.0%
! AAGGGGGTTTTTGTGAAGGA+AGG + Chr2:81375413-81375432 None:intergenic 45.0%
! CCATATAAGCCAGCTTTGTG+CGG + Chr2:81377698-81377717 None:intergenic 45.0%
! GACCTTTGCCTTTAAAAGCG+AGG + Chr2:81375727-81375746 None:intergenic 45.0%
! GACGCAGAATTTGGCTTATC+AGG - Chr2:81375934-81375953 MsG0280011241.01.T01:intron 45.0%
! GAGTAAGGGGGTTTTTGTGA+AGG + Chr2:81375417-81375436 None:intergenic 45.0%
! TTTGAGCCTTATCCGGTGTA+TGG + Chr2:81377781-81377800 None:intergenic 45.0%
!! ATTGGAGGTGTTAGGGTTTG+GGG + Chr2:81375657-81375676 None:intergenic 45.0%
!! CGTGACTTGGTCATACATGT+TGG - Chr2:81376728-81376747 MsG0280011241.01.T01:intron 45.0%
!! GAATTGGAGGTGTTAGGGTT+TGG + Chr2:81375659-81375678 None:intergenic 45.0%
!! GTGTTAGGGTTTGGGGTTTT+GGG + Chr2:81375650-81375669 None:intergenic 45.0%
!! TCCCTCGCTTTTAAAGGCAA+AGG - Chr2:81375722-81375741 MsG0280011241.01.T01:intron 45.0%
!! TGCGAATCCCTCGCTTTTAA+AGG - Chr2:81375716-81375735 MsG0280011241.01.T01:intron 45.0%
AGAGAGAGCAAAACGGTGAG+AGG + Chr2:81375570-81375589 None:intergenic 50.0%
AGCTGCACTGTAGAGTAAGG+GGG + Chr2:81375429-81375448 None:intergenic 50.0%
CAAATCGGGCTTGTAGGAAG+AGG + Chr2:81378128-81378147 None:intergenic 50.0%
CAATGTGGGGTTCCATACAC+CGG - Chr2:81377766-81377785 MsG0280011241.01.T01:CDS 50.0%
CACCCGTAATTGAGTCACCT+AGG + Chr2:81377605-81377624 None:intergenic 50.0%
CTAACTCAACCGCACAAAGC+TGG - Chr2:81377686-81377705 MsG0280011241.01.T01:CDS 50.0%
CTTCAGCAAATCGGGCTTGT+AGG + Chr2:81378134-81378153 None:intergenic 50.0%
CTTTCGTGCAGCGACTCTTT+AGG + Chr2:81376978-81376997 None:intergenic 50.0%
GAGAGAGCAAAACGGTGAGA+GGG + Chr2:81375569-81375588 None:intergenic 50.0%
GCACTTGGATTGCATGTTCC+TGG - Chr2:81377026-81377045 MsG0280011241.01.T01:intron 50.0%
GGAGGATTACTCGGAAGAAG+GGG - Chr2:81375816-81375835 MsG0280011241.01.T01:intron 50.0%
GGATTCGCAGAGTAGTTCGA+GGG + Chr2:81375705-81375724 None:intergenic 50.0%
GGGAGGATTACTCGGAAGAA+GGG - Chr2:81375815-81375834 MsG0280011241.01.T01:intron 50.0%
GTGCGGTTGAGTTAGGCATT+AGG + Chr2:81377681-81377700 None:intergenic 50.0%
TCTGCATTTCCCGAAGTGGT+TGG - Chr2:81378172-81378191 MsG0280011241.01.T01:five_prime_UTR 50.0%
TGGGAGGATTACTCGGAAGA+AGG - Chr2:81375814-81375833 MsG0280011241.01.T01:intron 50.0%
TGTTGGAGAGACCCACAACT+TGG - Chr2:81377268-81377287 MsG0280011241.01.T01:intron 50.0%
TTCCCGGTGAGCGTTTCATT+GGG - Chr2:81375795-81375814 MsG0280011241.01.T01:intron 50.0%
TTGGCCGCGTTTAGGGAAAT+AGG + Chr2:81375681-81375700 None:intergenic 50.0%
! CAGCTTTGTGCGGTTGAGTT+AGG + Chr2:81377688-81377707 None:intergenic 50.0%
! CCGCACAAAGCTGGCTTATA+TGG - Chr2:81377695-81377714 MsG0280011241.01.T01:CDS 50.0%
! GAAGTTGTGGTGGTTGGTTC+TGG + Chr2:81375623-81375642 None:intergenic 50.0%
! GGAGTGGGAGTGTGTTTGTT+AGG + Chr2:81375517-81375536 None:intergenic 50.0%
! GTGTGTTGAGTGTCGCACAT+TGG + Chr2:81377542-81377561 None:intergenic 50.0%
!! CTACAAGCCCGATTTGCTGA+AGG - Chr2:81378132-81378151 MsG0280011241.01.T01:five_prime_UTR 50.0%
!! GACTGGTCTGCCTGAAGTAA+TGG - Chr2:81378090-81378109 MsG0280011241.01.T01:CDS 50.0%
!! GGTGTTAGGGTTTGGGGTTT+TGG + Chr2:81375651-81375670 None:intergenic 50.0%
!! GGTTGGTTCTGGTTGTGGTT+CGG + Chr2:81375612-81375631 None:intergenic 50.0%
!! GTTGGTTCTGGTTGTGGTTC+GGG + Chr2:81375611-81375630 None:intergenic 50.0%
!! TTGGTTCTGGTTGTGGTTCG+GGG + Chr2:81375610-81375629 None:intergenic 50.0%
AAACGGTGAGAGGGAGAGGA+TGG + Chr2:81375560-81375579 None:intergenic 55.0%
AACGGTGAGAGGGAGAGGAT+GGG + Chr2:81375559-81375578 None:intergenic 55.0%
AGCAAAACGGTGAGAGGGAG+AGG + Chr2:81375564-81375583 None:intergenic 55.0%
ATGTGTGACCGTGCACTTCC+CGG - Chr2:81375779-81375798 MsG0280011241.01.T01:intron 55.0%
CACCTAGGTCACACTCAAGC+CGG + Chr2:81377590-81377609 None:intergenic 55.0%
CCTCCCAATGAAACGCTCAC+CGG + Chr2:81375801-81375820 None:intergenic 55.0%
CGCAGAGTAGTTCGAGGGTT+TGG + Chr2:81375700-81375719 None:intergenic 55.0%
CTCCCAATGAAACGCTCACC+GGG + Chr2:81375800-81375819 None:intergenic 55.0%
CTTCCCGGTGAGCGTTTCAT+TGG - Chr2:81375794-81375813 MsG0280011241.01.T01:intron 55.0%
GGGATTCGCAGAGTAGTTCG+AGG + Chr2:81375706-81375725 None:intergenic 55.0%
GGGGTTCCATACACCGGATA+AGG - Chr2:81377772-81377791 MsG0280011241.01.T01:CDS 55.0%
! TTCTGGTTGTGGTTCGGGGA+GGG + Chr2:81375606-81375625 None:intergenic 55.0%
!! GTGGTGGTTGGTTCTGGTTG+TGG + Chr2:81375617-81375636 None:intergenic 55.0%
AACACACACCTCAGACGCCC+AGG - Chr2:81377555-81377574 MsG0280011241.01.T01:intron 60.0%
ACACACACCTCAGACGCCCA+GGG - Chr2:81377556-81377575 MsG0280011241.01.T01:intron 60.0%
CAAGCCGGCTATTCCTCCCT+GGG + Chr2:81377575-81377594 None:intergenic 60.0%
CCGGTGAGCGTTTCATTGGG+AGG - Chr2:81375798-81375817 MsG0280011241.01.T01:intron 60.0%
CGAGGGTTTGGCCGCGTTTA+GGG + Chr2:81375688-81375707 None:intergenic 60.0%
TATTCCTCCCTGGGCGTCTG+AGG + Chr2:81377566-81377585 None:intergenic 60.0%
TCAAGCCGGCTATTCCTCCC+TGG + Chr2:81377576-81377595 None:intergenic 60.0%
TCGAGGGTTTGGCCGCGTTT+AGG + Chr2:81375689-81375708 None:intergenic 60.0%
! AACGCTCACCGGGAAGTGCA+CGG + Chr2:81375790-81375809 None:intergenic 60.0%
! GTTCTGGTTGTGGTTCGGGG+AGG + Chr2:81375607-81375626 None:intergenic 60.0%
!! AGCCGGCTTGAGTGTGACCT+AGG - Chr2:81377585-81377604 MsG0280011241.01.T01:intron 60.0%
GACGCCCAGGGAGGAATAGC+CGG - Chr2:81377568-81377587 MsG0280011241.01.T01:intron 65.0%
CACACCTCAGACGCCCAGGG+AGG - Chr2:81377559-81377578 MsG0280011241.01.T01:intron 70.0%
Chromosome Type Strat End Strand Name
Chr2 gene 81375407 81378201 81375407 ID=MsG0280011241.01;Name=MsG0280011241.01
Chr2 mRNA 81375407 81378201 81375407 ID=MsG0280011241.01.T01;Parent=MsG0280011241.01;Name=MsG0280011241.01.T01;_AED=0.42;_eAED=0.42;_QI=87|1|0.8|1|1|1|5|0|332
Chr2 exon 81375407 81375527 81375407 ID=MsG0280011241.01.T01:exon:2748;Parent=MsG0280011241.01.T01
Chr2 exon 81376252 81376361 81376252 ID=MsG0280011241.01.T01:exon:2747;Parent=MsG0280011241.01.T01
Chr2 exon 81376499 81376666 81376499 ID=MsG0280011241.01.T01:exon:2746;Parent=MsG0280011241.01.T01
Chr2 exon 81376773 81376883 81376773 ID=MsG0280011241.01.T01:exon:2745;Parent=MsG0280011241.01.T01
Chr2 exon 81377626 81378201 81377626 ID=MsG0280011241.01.T01:exon:2744;Parent=MsG0280011241.01.T01
Chr2 five_prime_UTR 81378115 81378201 81378115 ID=MsG0280011241.01.T01:five_prime_utr;Parent=MsG0280011241.01.T01
Chr2 CDS 81377626 81378114 81377626 ID=MsG0280011241.01.T01:cds;Parent=MsG0280011241.01.T01
Chr2 CDS 81376773 81376883 81376773 ID=MsG0280011241.01.T01:cds;Parent=MsG0280011241.01.T01
Chr2 CDS 81376499 81376666 81376499 ID=MsG0280011241.01.T01:cds;Parent=MsG0280011241.01.T01
Chr2 CDS 81376252 81376361 81376252 ID=MsG0280011241.01.T01:cds;Parent=MsG0280011241.01.T01
Chr2 CDS 81375407 81375527 81375407 ID=MsG0280011241.01.T01:cds;Parent=MsG0280011241.01.T01
Gene Sequence

>MsG0280011241.01.T01

ATGACAAGAACAATTTCAAACCTAACAAACACACTCCCACTCCATTTCCGAAAATTCAAATTCCCATCCTCTCCCTCTCACCGTTTTGCTCTCTCTACTCTCTCATGTTCCCTCCCCGAACCACAACCAGAACCAACCACCACAACTTCAATTCCCAAAACCCCAAACCCTAACACCTCCAATTCCTATTTCCCTAAACGCGGCCAAACCCTCGAACTACTCTGCGAATCCCTCGCTTTTAAAGGCAAAGGTCTATGCAAAGTTACTGATACCGGTTTTGTCGTCATGTGTGACCGTGCACTTCCCGGTGAGCGTTTCATTGGGAGGATTACTCGGAAGAAGGGGAATTATGCGGAAGCTACTAAGGTTGAGACTTTAACTCCTCCTTTTGATGTTGTTGATGCGCCTTGTGTTTATGTTCCGCATTGTGGTGGTTGTAAGACGCAGAATTTGGCTTATCAGGCTCAGATTAAAGCTAAAGAGGAACAGGTCCGTGACTTGGTCATACATGTTGGAAGGTTTTCTCAGAAAGACTTGGAATTGAATGGTATTATGAAACCGATTGTTCCTTGTGATATCCAGTTTCATTATAGAAACAAGATGGAATTCTCATTTGGTCCATATAAATGGTTGCCTAAAGAGTCGCTGCACGAAAGAAATGTTGATGCTGGGAGTGAAAATTATGCACTTGGATTGCATGTTCCTGGTTTTTTTGATAAAATTATAAATGTTGAAAAGTGCTTACTACAAACTGATCCTGCTAATAAGGTTCTTGCGGCTATACAAGAATGTTGGAGAGACCCACAACTTGGTTTCTCACCCTACAATGTTCATTCACACAAAGGATTTCTTAAGCATTTGATGCTGAGAAGTGGAAGGGATGTAATGACTGGTCTGCCTGAAGTAATGGTCAACTTTGTGACCTCTTCCTACAAGCCCGATTTGCTGAAGGTCCTAGTAGATAAAGTTTCTGCATTTCCCGAAGTGGTTGGTACTTAA
Protein sequence

>MsG0280011241.01.T01

MTRTISNLTNTLPLHFRKFKFPSSPSHRFALSTLSCSLPEPQPEPTTTTSIPKTPNPNTSNSYFPKRGQTLELLCESLAFKGKGLCKVTDTGFVVMCDRALPGERFIGRITRKKGNYAEATKVETLTPPFDVVDAPCVYVPHCGGCKTQNLAYQAQIKAKEEQVRDLVIHVGRFSQKDLELNGIMKPIVPCDIQFHYRNKMEFSFGPYKWLPKESLHERNVDAGSENYALGLHVPGFFDKIINVEKCLLQTDPANKVLAAIQECWRDPQLGFSPYNVHSHKGFLKHLMLRSGRDVMTGLPEVMVNFVTSSYKPDLLKVLVDKVSAFPEVVGT*