Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006465.01.T01 | XP_003593210.1 | 91.489 | 141 | 8 | 1 | 2 | 142 | 76 | 212 | 7.24E-74 | 235 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006465.01.T01 | G7INX2 | 91.489 | 141 | 8 | 1 | 2 | 142 | 76 | 212 | 3.46e-74 | 235 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048016.01 | MsG0280006465.01 | 0.800968 | 1.123529e-48 | 5.302582e-46 |
| MsG0080048845.01 | MsG0280006465.01 | 0.801634 | 8.207037e-49 | 3.938828e-46 |
| MsG0080048912.01 | MsG0280006465.01 | 0.812913 | 3.337205e-51 | 2.148500e-48 |
| MsG0180000854.01 | MsG0280006465.01 | 0.823507 | 1.334663e-53 | 1.151452e-50 |
| MsG0180001100.01 | MsG0280006465.01 | 0.803777 | 2.964217e-49 | 1.502627e-46 |
| MsG0180001130.01 | MsG0280006465.01 | 0.814430 | 1.547191e-51 | 1.037938e-48 |
| MsG0180001523.01 | MsG0280006465.01 | 0.800562 | 1.359428e-48 | 6.349854e-46 |
| MsG0180001880.01 | MsG0280006465.01 | 0.801885 | 7.290233e-49 | 3.521346e-46 |
| MsG0180003253.01 | MsG0280006465.01 | 0.818272 | 2.136957e-52 | 1.592204e-49 |
| MsG0280006465.01 | MsG0280008932.01 | 0.851071 | 1.126619e-60 | 2.253109e-57 |
| MsG0280006465.01 | MsG0280009447.01 | 0.807363 | 5.244271e-50 | 2.915205e-47 |
| MsG0280006465.01 | MsG0280010198.01 | 0.820652 | 6.126687e-53 | 4.876087e-50 |
| MsG0280006465.01 | MsG0280010254.01 | 0.800235 | 1.585079e-48 | 7.342545e-46 |
| MsG0280006465.01 | MsG0280010904.01 | 0.824794 | 6.655982e-54 | 5.956930e-51 |
| MsG0280006465.01 | MsG0280010975.01 | 0.811014 | 8.652791e-51 | 5.297097e-48 |
| MsG0280006465.01 | MsG0280011401.01 | 0.808752 | 2.653452e-50 | 1.529669e-47 |
| MsG0280006465.01 | MsG0380013050.01 | -0.815248 | 1.018738e-51 | 6.986758e-49 |
| MsG0280006465.01 | MsG0380014624.01 | 0.808137 | 3.589905e-50 | 2.036467e-47 |
| MsG0280006465.01 | MsG0380015545.01 | 0.804469 | 2.128129e-49 | 1.098125e-46 |
| MsG0280006465.01 | MsG0380015745.01 | 0.801660 | 8.109972e-49 | 3.894674e-46 |
| MsG0280006465.01 | MsG0380016762.01 | 0.811606 | 6.437032e-51 | 4.002059e-48 |
| MsG0280006465.01 | MsG0480021488.01 | 0.827382 | 1.613227e-54 | 1.555099e-51 |
| MsG0280006465.01 | MsG0480021737.01 | 0.851716 | 7.404475e-61 | 1.512155e-57 |
| MsG0280006465.01 | MsG0480021739.01 | 0.876412 | 1.425937e-68 | 6.906708e-65 |
| MsG0280006465.01 | MsG0480023565.01 | 0.823305 | 1.487560e-53 | 1.276017e-50 |
| MsG0280006465.01 | MsG0480023641.01 | 0.834887 | 2.311717e-56 | 2.780435e-53 |
| MsG0280006465.01 | MsG0580024192.01 | 0.805563 | 1.256674e-49 | 6.669655e-47 |
| MsG0280006465.01 | MsG0580026786.01 | -0.846261 | 2.427027e-59 | 4.153349e-56 |
| MsG0280006465.01 | MsG0580028353.01 | 0.844620 | 6.753916e-59 | 1.096634e-55 |
| MsG0280006465.01 | MsG0580028925.01 | 0.808330 | 3.266004e-50 | 1.862243e-47 |
| MsG0280006465.01 | MsG0580029414.01 | 0.802884 | 4.538575e-49 | 2.248636e-46 |
| MsG0280006465.01 | MsG0680030549.01 | 0.862026 | 6.782301e-64 | 1.957692e-60 |
| MsG0280006465.01 | MsG0680030964.01 | 0.863631 | 2.169459e-64 | 6.621431e-61 |
| MsG0280006465.01 | MsG0680032814.01 | 0.833255 | 5.922066e-56 | 6.782716e-53 |
| MsG0280006465.01 | MsG0680034235.01 | 0.803296 | 3.730952e-49 | 1.867979e-46 |
| MsG0280006465.01 | MsG0680035307.01 | 0.800403 | 1.464898e-48 | 6.815069e-46 |
| MsG0280006465.01 | MsG0680035308.01 | 0.820131 | 8.066777e-53 | 6.329374e-50 |
| MsG0280006465.01 | MsG0780036263.01 | 0.809165 | 2.165311e-50 | 1.262109e-47 |
| MsG0280006465.01 | MsG0780037399.01 | 0.805896 | 1.070010e-49 | 5.727936e-47 |
| MsG0280006465.01 | MsG0780037946.01 | 0.849655 | 2.812072e-60 | 5.368700e-57 |
| MsG0280006465.01 | MsG0780039975.01 | 0.804292 | 2.317173e-49 | 1.190274e-46 |
| MsG0280006465.01 | MsG0780039994.01 | 0.819293 | 1.253491e-52 | 9.606395e-50 |
| MsG0280006465.01 | MsG0880042931.01 | 0.816824 | 4.531358e-52 | 3.244913e-49 |
| MsG0280006465.01 | MsG0880044105.01 | 0.805706 | 1.172590e-49 | 6.247049e-47 |
| MsG0280006465.01 | MsG0880044884.01 | 0.814898 | 1.218301e-51 | 8.276458e-49 |
| MsG0280006465.01 | MsG0880047152.01 | 0.810960 | 8.889704e-51 | 5.434307e-48 |
| MsG0280006465.01 | MsG0880047519.01 | 0.811296 | 7.515956e-51 | 4.634799e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006465.01.T01 | MTR_2g008960 | 91.489 | 141 | 8 | 1 | 2 | 142 | 76 | 212 | 8.76e-78 | 235 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006465.01.T01 | AT5G64230 | 43.182 | 88 | 50 | 0 | 3 | 90 | 77 | 164 | 6.24e-15 | 70.9 |
Find 50 sgRNAs with CRISPR-Local
Find 53 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAGGAGGGTGTGGAGGGTGG+TGG | 0.218739 | 2:+2318905 | MsG0280006465.01.T01:CDS |
| GGGTGTGGAGGGTGGTGGGG+TGG | 0.285106 | 2:+2318910 | MsG0280006465.01.T01:CDS |
| GGAGGGTGTGGAGGGTGGTG+GGG | 0.287513 | 2:+2318907 | MsG0280006465.01.T01:CDS |
| GAGGAGGAGGGTGTGGAGGG+TGG | 0.291263 | 2:+2318902 | MsG0280006465.01.T01:CDS |
| TGGTTTAAGTGGTGGTGTTC+CGG | 0.299500 | 2:+2318953 | MsG0280006465.01.T01:CDS |
| GTGGAGGGTGGTGGGGTGGA+AGG | 0.327039 | 2:+2318914 | MsG0280006465.01.T01:CDS |
| AGGAGGGTGTGGAGGGTGGT+GGG | 0.327315 | 2:+2318906 | MsG0280006465.01.T01:CDS |
| GTGGAAGGTGAAGGGAGGTG+AGG | 0.381406 | 2:+2318929 | MsG0280006465.01.T01:CDS |
| GAATGGTGAGGAGGAGGGTG+TGG | 0.384248 | 2:+2318895 | MsG0280006465.01.T01:CDS |
| GTTGCTTTGTCTTCAGCGTT+TGG | 0.402044 | 2:+2318789 | MsG0280006465.01.T01:CDS |
| TGGTGTTCCGGCGGAGAAAT+TGG | 0.402368 | 2:+2318965 | MsG0280006465.01.T01:CDS |
| CGAAGTAGTTCGATCGTTAA+CGG | 0.422370 | 2:+2318707 | MsG0280006465.01.T01:CDS |
| GATCAAGGAGAGGGTGAAGA+AGG | 0.422863 | 2:+2318852 | MsG0280006465.01.T01:CDS |
| GGTGGTGGGGTGGAAGGTGA+AGG | 0.423207 | 2:+2318920 | MsG0280006465.01.T01:CDS |
| TTGCTTTGTCTTCAGCGTTT+GGG | 0.424552 | 2:+2318790 | MsG0280006465.01.T01:CDS |
| AAATTGGCAGCGGAGTTGTT+TGG | 0.427288 | 2:+2318981 | MsG0280006465.01.T01:CDS |
| GTGGGGTGGAAGGTGAAGGG+AGG | 0.443740 | 2:+2318924 | MsG0280006465.01.T01:CDS |
| TGGAAGGTGAAGGGAGGTGA+GGG | 0.448134 | 2:+2318930 | MsG0280006465.01.T01:CDS |
| TGGTGAGGAGGAGGGTGTGG+AGG | 0.454094 | 2:+2318898 | MsG0280006465.01.T01:CDS |
| GGTATAGAATGGTGAGGAGG+AGG | 0.456134 | 2:+2318889 | MsG0280006465.01.T01:CDS |
| GGTGAGGAGGAGGGTGTGGA+GGG | 0.497691 | 2:+2318899 | MsG0280006465.01.T01:CDS |
| AAGGTGGCGGAGAGAGATCA+AGG | 0.501719 | 2:+2318837 | MsG0280006465.01.T01:CDS |
| GGAGGTGAGGGTGGTTTAAG+TGG | 0.502354 | 2:+2318942 | MsG0280006465.01.T01:CDS |
| GAGGGTGAAGAAGGTGATGG+AGG | 0.506425 | 2:+2318861 | MsG0280006465.01.T01:CDS |
| CTTTGTCTTCAGCGTTTGGG+AGG | 0.507256 | 2:+2318793 | MsG0280006465.01.T01:CDS |
| GGAGAGGGTGAAGAAGGTGA+TGG | 0.514958 | 2:+2318858 | MsG0280006465.01.T01:CDS |
| GTGGTGGGGTGGAAGGTGAA+GGG | 0.517185 | 2:+2318921 | MsG0280006465.01.T01:CDS |
| GTATAGAATGGTGAGGAGGA+GGG | 0.528110 | 2:+2318890 | MsG0280006465.01.T01:CDS |
| TGGGAGGGCGTTGAGGCAGT+TGG | 0.535862 | 2:+2318809 | MsG0280006465.01.T01:CDS |
| TCCGCTGCCAATTTCTCCGC+CGG | 0.537453 | 2:-2318972 | None:intergenic |
| AAGGTGAAGGGAGGTGAGGG+TGG | 0.541781 | 2:+2318933 | MsG0280006465.01.T01:CDS |
| AAGAAGGTGATGGAGGAGAG+TGG | 0.541790 | 2:+2318868 | MsG0280006465.01.T01:CDS |
| CAGCGTTTGGGAGGGCGTTG+AGG | 0.545073 | 2:+2318802 | MsG0280006465.01.T01:CDS |
| TTTGTCTTCAGCGTTTGGGA+GGG | 0.563106 | 2:+2318794 | MsG0280006465.01.T01:CDS |
| AGTGGTATAGAATGGTGAGG+AGG | 0.571843 | 2:+2318886 | MsG0280006465.01.T01:CDS |
| GTAACGAATGAACACCTTCG+AGG | 0.580463 | 2:-2318752 | None:intergenic |
| ATCGATGATAATCACCTCGA+AGG | 0.591758 | 2:+2318738 | MsG0280006465.01.T01:CDS |
| GGTGAGGGTGGTTTAAGTGG+TGG | 0.593095 | 2:+2318945 | MsG0280006465.01.T01:CDS |
| GTTGAGGCAGTTGGAAGTGA+AGG | 0.595100 | 2:+2318818 | MsG0280006465.01.T01:CDS |
| GCGGAGAGAGATCAAGGAGA+GGG | 0.598505 | 2:+2318843 | MsG0280006465.01.T01:CDS |
| CTGAGAAAGAGCACGTCCGA+TGG | 0.614254 | 2:-2318640 | None:intergenic |
| GAGGCAGTTGGAAGTGAAGG+TGG | 0.615671 | 2:+2318821 | MsG0280006465.01.T01:CDS |
| TCCGGCGGAGAAATTGGCAG+CGG | 0.615724 | 2:+2318971 | MsG0280006465.01.T01:CDS |
| TGGAGGAGAGTGGTATAGAA+TGG | 0.621876 | 2:+2318878 | MsG0280006465.01.T01:CDS |
| GGCGGAGAGAGATCAAGGAG+AGG | 0.623247 | 2:+2318842 | MsG0280006465.01.T01:CDS |
| GCAGTTGGAAGTGAAGGTGG+CGG | 0.625405 | 2:+2318824 | MsG0280006465.01.T01:CDS |
| ACACGAAACGACACTGCCAT+CGG | 0.626709 | 2:+2318624 | MsG0280006465.01.T01:CDS |
| TGGCAGCGGAGTTGTTTGGA+TGG | 0.627820 | 2:+2318985 | MsG0280006465.01.T01:CDS |
| TTTAAGTGGTGGTGTTCCGG+CGG | 0.628276 | 2:+2318956 | MsG0280006465.01.T01:CDS |
| GAGAGTGGTATAGAATGGTG+AGG | 0.628530 | 2:+2318883 | MsG0280006465.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TCGTATTTTCTTGAACTAAC+TGG | - | Chr2:2318690-2318709 | None:intergenic | 30.0% |
| ! | GAACTTATTCTGCGTTTTGA+TGG | - | Chr2:2318595-2318614 | None:intergenic | 35.0% |
| ATCGATGATAATCACCTCGA+AGG | + | Chr2:2318738-2318757 | MsG0280006465.01.T01:CDS | 40.0% | |
| CGAAGTAGTTCGATCGTTAA+CGG | + | Chr2:2318707-2318726 | MsG0280006465.01.T01:CDS | 40.0% | |
| TTGCTTTGTCTTCAGCGTTT+GGG | + | Chr2:2318790-2318809 | MsG0280006465.01.T01:CDS | 40.0% | |
| !! | CTTATTCTGCGTTTTGATGG+TGG | - | Chr2:2318592-2318611 | None:intergenic | 40.0% |
| AAATTGGCAGCGGAGTTGTT+TGG | + | Chr2:2318981-2319000 | MsG0280006465.01.T01:CDS | 45.0% | |
| AGTGGTATAGAATGGTGAGG+AGG | + | Chr2:2318886-2318905 | MsG0280006465.01.T01:CDS | 45.0% | |
| GAGAGTGGTATAGAATGGTG+AGG | + | Chr2:2318883-2318902 | MsG0280006465.01.T01:CDS | 45.0% | |
| GTAACGAATGAACACCTTCG+AGG | - | Chr2:2318755-2318774 | None:intergenic | 45.0% | |
| GTATAGAATGGTGAGGAGGA+GGG | + | Chr2:2318890-2318909 | MsG0280006465.01.T01:CDS | 45.0% | |
| TGGAGGAGAGTGGTATAGAA+TGG | + | Chr2:2318878-2318897 | MsG0280006465.01.T01:CDS | 45.0% | |
| TTTGTCTTCAGCGTTTGGGA+GGG | + | Chr2:2318794-2318813 | MsG0280006465.01.T01:CDS | 45.0% | |
| ! | GTTGCTTTGTCTTCAGCGTT+TGG | + | Chr2:2318789-2318808 | MsG0280006465.01.T01:CDS | 45.0% |
| ! | TGGTTTAAGTGGTGGTGTTC+CGG | + | Chr2:2318953-2318972 | MsG0280006465.01.T01:CDS | 45.0% |
| ACACGAAACGACACTGCCAT+CGG | + | Chr2:2318624-2318643 | MsG0280006465.01.T01:CDS | 50.0% | |
| CTTTGTCTTCAGCGTTTGGG+AGG | + | Chr2:2318793-2318812 | MsG0280006465.01.T01:CDS | 50.0% | |
| GATCAAGGAGAGGGTGAAGA+AGG | + | Chr2:2318852-2318871 | MsG0280006465.01.T01:CDS | 50.0% | |
| GGTATAGAATGGTGAGGAGG+AGG | + | Chr2:2318889-2318908 | MsG0280006465.01.T01:CDS | 50.0% | |
| GTTGAGGCAGTTGGAAGTGA+AGG | + | Chr2:2318818-2318837 | MsG0280006465.01.T01:CDS | 50.0% | |
| ! | TTTAAGTGGTGGTGTTCCGG+CGG | + | Chr2:2318956-2318975 | MsG0280006465.01.T01:CDS | 50.0% |
| !! | AAGAAGGTGATGGAGGAGAG+TGG | + | Chr2:2318868-2318887 | MsG0280006465.01.T01:CDS | 50.0% |
| GAGGCAGTTGGAAGTGAAGG+TGG | + | Chr2:2318821-2318840 | MsG0280006465.01.T01:CDS | 55.0% | |
| GCAGTTGGAAGTGAAGGTGG+CGG | + | Chr2:2318824-2318843 | MsG0280006465.01.T01:CDS | 55.0% | |
| GCGGAGAGAGATCAAGGAGA+GGG | + | Chr2:2318843-2318862 | MsG0280006465.01.T01:CDS | 55.0% | |
| GGAGAGGGTGAAGAAGGTGA+TGG | + | Chr2:2318858-2318877 | MsG0280006465.01.T01:CDS | 55.0% | |
| GGAGGTGAGGGTGGTTTAAG+TGG | + | Chr2:2318942-2318961 | MsG0280006465.01.T01:CDS | 55.0% | |
| GGTGAGGGTGGTTTAAGTGG+TGG | + | Chr2:2318945-2318964 | MsG0280006465.01.T01:CDS | 55.0% | |
| TGGAAGGTGAAGGGAGGTGA+GGG | + | Chr2:2318930-2318949 | MsG0280006465.01.T01:CDS | 55.0% | |
| TGGCAGCGGAGTTGTTTGGA+TGG | + | Chr2:2318985-2319004 | MsG0280006465.01.T01:CDS | 55.0% | |
| TGGTGTTCCGGCGGAGAAAT+TGG | + | Chr2:2318965-2318984 | MsG0280006465.01.T01:CDS | 55.0% | |
| ! | CTGAGAAAGAGCACGTCCGA+TGG | - | Chr2:2318643-2318662 | None:intergenic | 55.0% |
| ! | GAGGGTGAAGAAGGTGATGG+AGG | + | Chr2:2318861-2318880 | MsG0280006465.01.T01:CDS | 55.0% |
| !! | AAGGTGGCGGAGAGAGATCA+AGG | + | Chr2:2318837-2318856 | MsG0280006465.01.T01:CDS | 55.0% |
| AAGGTGAAGGGAGGTGAGGG+TGG | + | Chr2:2318933-2318952 | MsG0280006465.01.T01:CDS | 60.0% | |
| GAATGGTGAGGAGGAGGGTG+TGG | + | Chr2:2318895-2318914 | MsG0280006465.01.T01:CDS | 60.0% | |
| GGCGGAGAGAGATCAAGGAG+AGG | + | Chr2:2318842-2318861 | MsG0280006465.01.T01:CDS | 60.0% | |
| GTGGAAGGTGAAGGGAGGTG+AGG | + | Chr2:2318929-2318948 | MsG0280006465.01.T01:CDS | 60.0% | |
| TCCGCTGCCAATTTCTCCGC+CGG | - | Chr2:2318975-2318994 | None:intergenic | 60.0% | |
| TCCGGCGGAGAAATTGGCAG+CGG | + | Chr2:2318971-2318990 | MsG0280006465.01.T01:CDS | 60.0% | |
| ! | GTGGTGGGGTGGAAGGTGAA+GGG | + | Chr2:2318921-2318940 | MsG0280006465.01.T01:CDS | 60.0% |
| GTGGGGTGGAAGGTGAAGGG+AGG | + | Chr2:2318924-2318943 | MsG0280006465.01.T01:CDS | 65.0% | |
| ! | AGGAGGGTGTGGAGGGTGGT+GGG | + | Chr2:2318906-2318925 | MsG0280006465.01.T01:CDS | 65.0% |
| ! | GGTGAGGAGGAGGGTGTGGA+GGG | + | Chr2:2318899-2318918 | MsG0280006465.01.T01:CDS | 65.0% |
| ! | TGGTGAGGAGGAGGGTGTGG+AGG | + | Chr2:2318898-2318917 | MsG0280006465.01.T01:CDS | 65.0% |
| !! | CAGCGTTTGGGAGGGCGTTG+AGG | + | Chr2:2318802-2318821 | MsG0280006465.01.T01:CDS | 65.0% |
| !! | GGTGGTGGGGTGGAAGGTGA+AGG | + | Chr2:2318920-2318939 | MsG0280006465.01.T01:CDS | 65.0% |
| !! | TGGGAGGGCGTTGAGGCAGT+TGG | + | Chr2:2318809-2318828 | MsG0280006465.01.T01:CDS | 65.0% |
| ! | GAGGAGGAGGGTGTGGAGGG+TGG | + | Chr2:2318902-2318921 | MsG0280006465.01.T01:CDS | 70.0% |
| ! | GAGGAGGGTGTGGAGGGTGG+TGG | + | Chr2:2318905-2318924 | MsG0280006465.01.T01:CDS | 70.0% |
| ! | GGAGGGTGTGGAGGGTGGTG+GGG | + | Chr2:2318907-2318926 | MsG0280006465.01.T01:CDS | 70.0% |
| !! | GTGGAGGGTGGTGGGGTGGA+AGG | + | Chr2:2318914-2318933 | MsG0280006465.01.T01:CDS | 70.0% |
| !! | GGGTGTGGAGGGTGGTGGGG+TGG | + | Chr2:2318910-2318929 | MsG0280006465.01.T01:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 2318573 | 2319010 | 2318573 | ID=MsG0280006465.01;Name=MsG0280006465.01 |
| Chr2 | mRNA | 2318573 | 2319010 | 2318573 | ID=MsG0280006465.01.T01;Parent=MsG0280006465.01;Name=MsG0280006465.01.T01;_AED=0.46;_eAED=0.46;_QI=0|-1|0|1|-1|1|1|0|145 |
| Chr2 | exon | 2318573 | 2319010 | 2318573 | ID=MsG0280006465.01.T01:exon:1417;Parent=MsG0280006465.01.T01 |
| Chr2 | CDS | 2318573 | 2319010 | 2318573 | ID=MsG0280006465.01.T01:cds;Parent=MsG0280006465.01.T01 |
| Gene Sequence |
| Protein sequence |
