AlfalfaGEDB | MsG0480022041.01

M. sativa cultivar ZhongmuNo.1 / MsG0480022041.01

KEGG: K02979
GO: GO:0003735 GO:0005622 GO:0005840 GO:0006412
PFAM: PF01200

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480022041.01.T01	XP_024637297.1	93.846	65	4	0	8	72	1	65	1.96E-35	124

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480022041.01.T01	Q9SR73	87.692	65	7	1	8	72	1	64	2.09E-33	112

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480022041.01.T01	G7JF15	93.939	66	4	0	7	72	149	214	7.33e-35	126

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180000118.01	MsG0480022041.01	0.860146	2.530982e-63	6.849864e-60
MsG0180001829.01	MsG0480022041.01	0.815745	7.897124e-52	5.491118e-49
MsG0180003897.01	MsG0480022041.01	0.850737	1.399556e-60	2.768365e-57
MsG0180004829.01	MsG0480022041.01	0.888008	8.638768e-73	6.575681e-69
MsG0180005384.01	MsG0480022041.01	0.870700	1.196608e-66	4.698870e-63
MsG0280011308.01	MsG0480022041.01	0.816103	6.571345e-52	4.614000e-49
MsG0480019165.01	MsG0480022041.01	0.844438	7.559166e-59	1.220303e-55
MsG0480020796.01	MsG0480022041.01	0.861660	8.776625e-64	2.501631e-60
MsG0480020911.01	MsG0480022041.01	0.861723	8.396074e-64	2.397918e-60
MsG0480021279.01	MsG0480022041.01	0.866573	2.582875e-65	8.747009e-62
MsG0480021417.01	MsG0480022041.01	0.825345	4.931716e-54	4.483230e-51
MsG0480022041.01	MsG0480022789.01	0.832798	7.694714e-56	8.693152e-53
MsG0480022041.01	MsG0480022955.01	0.803399	3.551844e-49	1.783115e-46
MsG0480022041.01	MsG0480023380.01	0.849133	3.930803e-60	7.380120e-57
MsG0480022041.01	MsG0480023774.01	0.816540	5.246837e-52	3.728079e-49
MsG0480022041.01	MsG0580024481.01	0.803675	3.113582e-49	1.574124e-46
MsG0480022041.01	MsG0580024540.01	0.825951	3.543005e-54	3.277095e-51
MsG0480022041.01	MsG0580024894.01	0.816147	6.424005e-52	4.515911e-49
MsG0480022041.01	MsG0680031368.01	0.821268	4.419325e-53	3.578175e-50
MsG0480022041.01	MsG0680035611.01	0.821480	3.949365e-53	3.216708e-50
MsG0480022041.01	MsG0780037290.01	0.812309	4.522690e-51	2.865705e-48
MsG0480022041.01	MsG0780038383.01	0.843494	1.354103e-58	2.122281e-55
MsG0480022041.01	MsG0780041058.01	0.808895	2.473459e-50	1.431233e-47
MsG0480022041.01	MsG0780041153.01	0.816500	5.357023e-52	3.802087e-49
MsG0480022041.01	MsG0780041522.01	0.803911	2.780289e-49	1.414347e-46
MsG0480022041.01	MsG0780041790.01	0.800511	1.392790e-48	6.497101e-46
MsG0480022041.01	MsG0880041941.01	0.827937	1.187275e-54	1.162979e-51
MsG0480022041.01	MsG0880042517.01	0.848865	4.666746e-60	8.684155e-57
MsG0480022041.01	MsG0880046220.01	0.839062	1.984038e-57	2.708354e-54
MsG0380015857.01	MsG0480022041.01	0.835449	1.666869e-56	2.038998e-53
MsG0380016678.01	MsG0480022041.01	0.828687	7.825521e-55	7.834700e-52
MsG0480018164.01	MsG0480022041.01	0.886066	4.725614e-72	3.324368e-68
MsG0280006629.01	MsG0480022041.01	0.856785	2.544335e-62	6.141472e-59
MsG0280006747.01	MsG0480022041.01	0.810496	1.119992e-50	6.761381e-48
MsG0280006841.01	MsG0480022041.01	0.814652	1.381456e-51	9.323167e-49
MsG0280007055.01	MsG0480022041.01	0.898696	4.117340e-77	4.922414e-73

PPI

Gene1	Gene2	Type
MsG0280010383.01	MsG0480022041.01	PPI
MsG0480021288.01	MsG0480022041.01	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480022041.01.T01	MTR_4g081180	93.939	66	4	0	7	72	149	214	1.86e-38	126
MsG0480022041.01.T01	MTR_6g013210	90.769	65	6	0	8	72	1	65	1.43e-37	119
MsG0480022041.01.T01	MTR_6g013410	74.242	66	16	1	7	72	37	101	7.32e-28	96.7
MsG0480022041.01.T01	MTR_8g089765	70.000	60	18	0	8	67	1	60	4.33e-25	89.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480022041.01.T01	AT5G03850	87.692	65	7	1	8	72	1	64	2.13e-34	112
MsG0480022041.01.T01	AT3G10090	87.692	65	7	1	8	72	1	64	2.13e-34	112
MsG0480022041.01.T01	AT5G64140	87.692	65	7	1	8	72	1	64	5.49e-34	110

Find 25 sgRNAs with CRISPR-Local

Find 23 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGGGTGTCTCCTTCTCTAAC+TGG	0.224700	4:+67283054	None:intergenic
TGCTGTTGTTGTCAAGGTTA+TGG	0.305748	4:-67283166	MsG0480022041.01.T01:CDS
GCTGTTGTTGTCAAGGTTAT+GGG	0.344081	4:-67283165	MsG0480022041.01.T01:CDS
GAGGACAGGTGACTCAAGTT+AGG	0.354183	4:-67283128	MsG0480022041.01.T01:CDS
TTCCTCATGATGTGACGGTT+CGG	0.361521	4:+67283087	None:intergenic
TCCTCATGATGTGACGGTTC+GGG	0.372907	4:+67283088	None:intergenic
CTTCTCTTTCAGACTCAAGC+AGG	0.393026	4:+67283028	None:intergenic
GTCAAGGTTATGGGTCGTAC+TGG	0.486004	4:-67283156	MsG0480022041.01.T01:CDS
TTTCAGACTCAAGCAGGGTG+AGG	0.496062	4:+67283034	None:intergenic
GTGCTTGTGTTTGAGCAGAA+TGG	0.506628	4:-67283205	MsG0480022041.01.T01:CDS
AGAAGCAAGAAGGTTGCGCT+AGG	0.520652	4:-67283010	None:intergenic
GGGTGTCTCCTTCTCTAACT+GGG	0.522501	4:+67283055	None:intergenic
AGGACAGGTGACTCAAGTTA+GGG	0.535175	4:-67283127	MsG0480022041.01.T01:CDS
CAAGTATGCTGTTGTTGTCA+AGG	0.536125	4:-67283172	MsG0480022041.01.T01:CDS
TCGTACTGGTTCTAGAGGAC+AGG	0.538891	4:-67283142	MsG0480022041.01.T01:CDS
TTCAGACTCAAGCAGGGTGA+GGG	0.547860	4:+67283035	None:intergenic
ACCCGAACCGTCACATCATG+AGG	0.555528	4:-67283089	MsG0480022041.01.T01:CDS
TTCTCTTTCAGACTCAAGCA+GGG	0.585301	4:+67283029	None:intergenic
TCACATCATGAGGAATGTGA+AGG	0.587194	4:-67283079	MsG0480022041.01.T01:CDS
TCACATTCCTCATGATGTGA+CGG	0.589714	4:+67283082	None:intergenic
CACATCATGAGGAATGTGAA+GGG	0.593653	4:-67283078	MsG0480022041.01.T01:CDS
GAAGCAAGAAGGTTGCGCTA+GGG	0.598738	4:-67283009	None:intergenic
GTGAAGGGCCCAGTTAGAGA+AGG	0.598823	4:-67283063	MsG0480022041.01.T01:CDS
AGTCTGAAAGAGAAGCAAGA+AGG	0.599710	4:-67283020	MsG0480022041.01.T01:CDS
ATGGGTCGTACTGGTTCTAG+AGG	0.630812	4:-67283147	MsG0480022041.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AGTCTGAAAGAGAAGCAAGA+AGG	-	Chr4:67283198-67283217	MsG0480022041.01.T01:CDS	40.0%
	CAAGTATGCTGTTGTTGTCA+AGG	-	Chr4:67283046-67283065	MsG0480022041.01.T01:CDS	40.0%
	CACATCATGAGGAATGTGAA+GGG	-	Chr4:67283140-67283159	MsG0480022041.01.T01:CDS	40.0%
	GCTGTTGTTGTCAAGGTTAT+GGG	-	Chr4:67283053-67283072	MsG0480022041.01.T01:CDS	40.0%
	TCACATCATGAGGAATGTGA+AGG	-	Chr4:67283139-67283158	MsG0480022041.01.T01:CDS	40.0%
	TCACATTCCTCATGATGTGA+CGG	+	Chr4:67283139-67283158	None:intergenic	40.0%
	TGCTGTTGTTGTCAAGGTTA+TGG	-	Chr4:67283052-67283071	MsG0480022041.01.T01:CDS	40.0%
	TTCTCTTTCAGACTCAAGCA+GGG	+	Chr4:67283192-67283211	None:intergenic	40.0%
	AGGACAGGTGACTCAAGTTA+GGG	-	Chr4:67283091-67283110	MsG0480022041.01.T01:CDS	45.0%
	CTTCTCTTTCAGACTCAAGC+AGG	+	Chr4:67283193-67283212	None:intergenic	45.0%
	GTGCTTGTGTTTGAGCAGAA+TGG	-	Chr4:67283013-67283032	MsG0480022041.01.T01:CDS	45.0%
	TTCCTCATGATGTGACGGTT+CGG	+	Chr4:67283134-67283153	None:intergenic	45.0%
	GAGGACAGGTGACTCAAGTT+AGG	-	Chr4:67283090-67283109	MsG0480022041.01.T01:CDS	50.0%
	GGGTGTCTCCTTCTCTAACT+GGG	+	Chr4:67283166-67283185	None:intergenic	50.0%
	GTCAAGGTTATGGGTCGTAC+TGG	-	Chr4:67283062-67283081	MsG0480022041.01.T01:CDS	50.0%
	TCCTCATGATGTGACGGTTC+GGG	+	Chr4:67283133-67283152	None:intergenic	50.0%
	TTCAGACTCAAGCAGGGTGA+GGG	+	Chr4:67283186-67283205	None:intergenic	50.0%
	TTTCAGACTCAAGCAGGGTG+AGG	+	Chr4:67283187-67283206	None:intergenic	50.0%
!	AGGGTGTCTCCTTCTCTAAC+TGG	+	Chr4:67283167-67283186	None:intergenic	50.0%
!!	ATGGGTCGTACTGGTTCTAG+AGG	-	Chr4:67283071-67283090	MsG0480022041.01.T01:CDS	50.0%
!!	TCGTACTGGTTCTAGAGGAC+AGG	-	Chr4:67283076-67283095	MsG0480022041.01.T01:CDS	50.0%
	ACCCGAACCGTCACATCATG+AGG	-	Chr4:67283129-67283148	MsG0480022041.01.T01:CDS	55.0%
	GTGAAGGGCCCAGTTAGAGA+AGG	-	Chr4:67283155-67283174	MsG0480022041.01.T01:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	67283011	67283229	67283011	ID=MsG0480022041.01;Name=MsG0480022041.01
Chr4	mRNA	67283011	67283229	67283011	ID=MsG0480022041.01.T01;Parent=MsG0480022041.01;Name=MsG0480022041.01.T01;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|72
Chr4	exon	67283011	67283229	67283011	ID=MsG0480022041.01.T01:exon:22468;Parent=MsG0480022041.01.T01
Chr4	CDS	67283011	67283229	67283011	ID=MsG0480022041.01.T01:cds;Parent=MsG0480022041.01.T01

Gene Sequence	>MsG0480022041.01.T01 ATGTGCTTGTGTTTGAGCAGAATGGATTCTCAAGTCAAGTATGCTGTTGTTGTCAAGGTTATGGGTCGTACTGGTTCTAGAGGACAGGTGACTCAAGTTAGGGTCAAGTTTAATGACGACCCGAACCGTCACATCATGAGGAATGTGAAGGGCCCAGTTAGAGAAGGAGACACCCTCACCCTGCTTGAGTCTGAAAGAGAAGCAAGAAGGTTGCGCTAG
Protein sequence	>MsG0480022041.01.T01 MCLCLSRMDSQVKYAVVVKVMGRTGSRGQVTQVRVKFNDDPNRHIMRNVKGPVREGDTLTLLESEREARRLR*