AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280010383.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280010383.01.T01 MTR_6g013210 100.000 65 0 0 28 92 1 65 2.43e-41 130
MsG0280010383.01.T01 MTR_4g081180 72.826 92 16 3 7 92 126 214 1.53e-35 120
MsG0280010383.01.T01 MTR_6g013410 81.538 65 11 1 28 92 38 101 1.46e-30 104
MsG0280010383.01.T01 MTR_8g089765 71.667 60 17 0 28 87 1 60 2.16e-24 89.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280010383.01.T01 AT5G03850 90.769 65 5 1 28 92 1 64 4.11e-35 114
MsG0280010383.01.T01 AT3G10090 90.769 65 5 1 28 92 1 64 4.11e-35 114
MsG0280010383.01.T01 AT5G64140 89.231 65 6 1 28 92 1 64 3.23e-34 112

Find 29 sgRNAs with CRISPR-Local

Find 114 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACCTGGACAATCCTTGATC+AGG 0.417303 2:-68840030 MsG0280010383.01.T01:CDS
GCTGTTGTTGTGAAAGTTAT+GGG 0.420809 2:-68838094 MsG0280010383.01.T01:CDS
AGGATGTCTCCCTCCCTAAC+TGG 0.425936 2:+68837983 None:intergenic
TGCTGTTGTTGTGAAAGTTA+TGG 0.441002 2:-68838095 MsG0280010383.01.T01:CDS
TTATGGGTCGTACTGGATCC+AGG 0.451262 2:-68838078 MsG0280010383.01.T01:CDS
AACCTGAGTGACTTGTCCCC+TGG 0.461687 2:+68838060 None:intergenic
GAATGTGAAGGGACCAGTTA+GGG 0.464736 2:-68837996 MsG0280010383.01.T01:CDS
GGAATGTGAAGGGACCAGTT+AGG 0.473428 2:-68837997 MsG0280010383.01.T01:CDS
GGGGAGACATATTCCGACTT+TGG 0.485899 2:-68840009 MsG0280010383.01.T01:intron
TCTCAGACTCGAGGAGGGTA+AGG 0.488938 2:+68837963 None:intergenic
GAATATGTCTCCCCTGATCA+AGG 0.522079 2:+68840018 MsG0280010382.01.T01:intron
GTGAAAGTTATGGGTCGTAC+TGG 0.530087 2:-68838085 MsG0280010383.01.T01:CDS
ACCTGGACAATCCTTGATCA+GGG 0.533449 2:-68840029 MsG0280010383.01.T01:CDS
TGAACTCAACATACCAAAGT+CGG 0.549702 2:+68839996 MsG0280010382.01.T01:intron
GAATTCCTCGCAATAGTACC+TGG 0.552253 2:-68840046 MsG0280010383.01.T01:CDS
CCCCTGATCAAGGATTGTCC+AGG 0.575343 2:+68840028 MsG0280010382.01.T01:intron
TGTGAAGGGACCAGTTAGGG+AGG 0.582336 2:-68837993 MsG0280010383.01.T01:CDS
ATCAGAATCGTCACATCATG+AGG 0.584464 2:-68838018 MsG0280010383.01.T01:CDS
TTGCTTCTCTCTCAGACTCG+AGG 0.586554 2:+68837954 None:intergenic
CTTCTCTCTCAGACTCGAGG+AGG 0.606831 2:+68837957 None:intergenic
TATGGGTCGTACTGGATCCA+GGG 0.613108 2:-68838077 MsG0280010383.01.T01:CDS
TCACATCATGAGGAATGTGA+AGG 0.626309 2:-68838008 MsG0280010383.01.T01:CDS
CACATCATGAGGAATGTGAA+GGG 0.632733 2:-68838007 MsG0280010383.01.T01:CDS
ATCCAGGGGACAAGTCACTC+AGG 0.638739 2:-68838062 MsG0280010383.01.T01:CDS
TTCTCTCTCAGACTCGAGGA+GGG 0.644799 2:+68837958 None:intergenic
GTGAAGGGACCAGTTAGGGA+GGG 0.647685 2:-68837992 MsG0280010383.01.T01:CDS
ATGGGTCGTACTGGATCCAG+GGG 0.674814 2:-68838076 MsG0280010383.01.T01:CDS
ATTGTCCAGGTACTATTGCG+AGG 0.675328 2:+68840041 MsG0280010382.01.T01:intron
CCTGGACAATCCTTGATCAG+GGG 0.690809 2:-68840028 MsG0280010383.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTATTTTGATTTTTTCA+TGG - Chr2:68839093-68839112 MsG0280010383.01.T01:intron 10.0%
!! AAATTATGATTTAAATAGTC+TGG - Chr2:68839734-68839753 MsG0280010383.01.T01:intron 15.0%
!! ATTTGAACAAATTTAAGAAT+AGG + Chr2:68838128-68838147 None:intergenic 15.0%
!!! ATAAATAAAGTTTTGTTGAA+GGG + Chr2:68838376-68838395 None:intergenic 15.0%
!!! TATAAATAAAGTTTTGTTGA+AGG + Chr2:68838377-68838396 None:intergenic 15.0%
!! AGTTAAAAAAGTCATTTCTA+AGG + Chr2:68839426-68839445 None:intergenic 20.0%
!! ATAGAGGAATAACATTTATT+TGG - Chr2:68838810-68838829 MsG0280010383.01.T01:intron 20.0%
!! GCTAAAAATATAGATCAATA+AGG + Chr2:68839028-68839047 None:intergenic 20.0%
!! TAGAGGAATAACATTTATTT+GGG - Chr2:68838811-68838830 MsG0280010383.01.T01:intron 20.0%
!!! ATCAACTTCACTTTATTTTT+TGG + Chr2:68839701-68839720 MsG0280010382.01.T01:CDS 20.0%
!!! TAAATAAAGTTTTGTTGAAG+GGG + Chr2:68838375-68838394 None:intergenic 20.0%
!!! TTTTAAACAAATTGCTTATG+TGG + Chr2:68838510-68838529 None:intergenic 20.0%
! AAATCTGAATAAGGAAAGTT+AGG - Chr2:68838891-68838910 MsG0280010383.01.T01:intron 25.0%
! AACTTTCCTTATTCAGATTT+GGG + Chr2:68838891-68838910 None:intergenic 25.0%
! AATCTGAATAAGGAAAGTTA+GGG - Chr2:68838892-68838911 MsG0280010383.01.T01:intron 25.0%
! ACACATTCACAATTGTTAAT+AGG + Chr2:68839859-68839878 MsG0280010382.01.T01:intron 25.0%
! CCAATATTAATTACTCTACA+TGG + Chr2:68838218-68838237 None:intergenic 25.0%
! CCATGTAGAGTAATTAATAT+TGG - Chr2:68838215-68838234 MsG0280010383.01.T01:intron 25.0%
! TAACTTTCCTTATTCAGATT+TGG + Chr2:68838892-68838911 None:intergenic 25.0%
! TATATACCCAAATCTGAATA+AGG - Chr2:68838882-68838901 MsG0280010383.01.T01:intron 25.0%
! TCAACATGATAGAAAAAAGA+AGG - Chr2:68838148-68838167 MsG0280010383.01.T01:intron 25.0%
! TTATTCCTCTATTCAGAAAA+TGG + Chr2:68838802-68838821 None:intergenic 25.0%
! TTGAGTTCAAAATACAAAGT+CGG - Chr2:68838025-68838044 MsG0280010383.01.T01:CDS 25.0%
! TTGATTGCTTATATTGTGTT+TGG - Chr2:68839650-68839669 MsG0280010383.01.T01:intron 25.0%
! TTGGGTATATATAGTAATGA+TGG + Chr2:68838873-68838892 None:intergenic 25.0%
!! ACCAAAAAAGTACAAATTCA+AGG - Chr2:68838741-68838760 MsG0280010383.01.T01:intron 25.0%
!! AGTAATGATGGTTAGATTTT+TGG + Chr2:68838861-68838880 None:intergenic 25.0%
!! CCAAAAAAGTACAAATTCAA+GGG - Chr2:68838742-68838761 MsG0280010383.01.T01:intron 25.0%
!! GTAATGATGGTTAGATTTTT+GGG + Chr2:68838860-68838879 None:intergenic 25.0%
!! GTTTGATTTCAACAAATTCA+TGG - Chr2:68838071-68838090 MsG0280010383.01.T01:CDS 25.0%
!! TGATTGCTATTAATTGTGTT+TGG - Chr2:68839457-68839476 MsG0280010383.01.T01:intron 25.0%
!! TTTAAAATGTGTGAAAGATG+AGG - Chr2:68839277-68839296 MsG0280010383.01.T01:intron 25.0%
!! TTTTCGAATAACAATCATGA+AGG + Chr2:68838352-68838371 None:intergenic 25.0%
!!! GATAAAAAAGTGTTTTTCAG+TGG - Chr2:68839770-68839789 MsG0280010383.01.T01:intron 25.0%
!!! GATTTTTTCATGGTTGATTT+AGG - Chr2:68839103-68839122 MsG0280010383.01.T01:intron 25.0%
!!! TTTTTGGTATTAACCTTCAT+GGG + Chr2:68838729-68838748 None:intergenic 25.0%
!!! TTTTTTGGTATTAACCTTCA+TGG + Chr2:68838730-68838749 None:intergenic 25.0%
AAACAACTTAATCAGAAGAC+TGG + Chr2:68838055-68838074 None:intergenic 30.0%
AGAATTTCTACAATGGTGAT+TGG + Chr2:68838185-68838204 None:intergenic 30.0%
CCAACCCATAAATAAGAATA+AGG - Chr2:68838834-68838853 MsG0280010383.01.T01:intron 30.0%
GTCGAGATGTAAGTAAAATA+TGG + Chr2:68838680-68838699 None:intergenic 30.0%
TATTAACCTTCATGGGAAAA+GGG + Chr2:68838722-68838741 None:intergenic 30.0%
! AATTACTCTACATGGATTGA+AGG + Chr2:68838210-68838229 None:intergenic 30.0%
! CCTTATTCTTATTTATGGGT+TGG + Chr2:68838837-68838856 None:intergenic 30.0%
! GAGGAATTCAATGTTTTGTA+TGG + Chr2:68837950-68837969 None:intergenic 30.0%
! TAAAATGGAAAGTGCAGAAT+CGG + Chr2:68838965-68838984 None:intergenic 30.0%
!! CAAATTCATGGTTTTGCAAT+TGG - Chr2:68838083-68838102 MsG0280010383.01.T01:CDS 30.0%
!! TGGGCCTTATTCTTATTTAT+GGG + Chr2:68838841-68838860 None:intergenic 30.0%
!! TTGGGCCTTATTCTTATTTA+TGG + Chr2:68838842-68838861 None:intergenic 30.0%
!! TTTTCATGGTTGATTTAGGT+TGG - Chr2:68839107-68839126 MsG0280010383.01.T01:intron 30.0%
!!! CCCTTGAATTTGTACTTTTT+TGG + Chr2:68838745-68838764 None:intergenic 30.0%
!!! TATTTTTTACCTTCCTAGCT+AGG - Chr2:68838414-68838433 MsG0280010383.01.T01:intron 30.0%
CAATTGTTAATAGGAGAGCA+AGG + Chr2:68839850-68839869 MsG0280010382.01.T01:intron 35.0%
CATGAAGGAGATAAAGTTGT+TGG + Chr2:68838337-68838356 None:intergenic 35.0%
CTTTACTAGACAATTGTGTG+AGG + Chr2:68838611-68838630 None:intergenic 35.0%
GCTGTTGTTGTGAAAGTTAT+GGG - Chr2:68839913-68839932 MsG0280010383.01.T01:intron 35.0%
GTATTAACCTTCATGGGAAA+AGG + Chr2:68838723-68838742 None:intergenic 35.0%
GTGCATTTGTAGAAAGTCAA+TGG + Chr2:68838468-68838487 None:intergenic 35.0%
TCAACAAATTAAGCTGAAGC+TGG + Chr2:68839362-68839381 None:intergenic 35.0%
TGAACTCAACATACCAAAGT+CGG + Chr2:68838014-68838033 None:intergenic 35.0%
TGCTGTTGTTGTGAAAGTTA+TGG - Chr2:68839912-68839931 MsG0280010383.01.T01:intron 35.0%
! CTTGAAGTTGGTTGCTAAAA+TGG + Chr2:68838980-68838999 None:intergenic 35.0%
! GTGAACCATTTTCTGAATAG+AGG - Chr2:68838794-68838813 MsG0280010383.01.T01:intron 35.0%
! GTTGCTTCTTGTTATGTTGT+CGG + Chr2:68838289-68838308 None:intergenic 35.0%
! TCAGGTTAGAGTGAAGTTTT+TGG - Chr2:68839963-68839982 MsG0280010383.01.T01:intron 35.0%
!! ATTCATGGTTTTGCAATTGG+AGG - Chr2:68838086-68838105 MsG0280010383.01.T01:CDS 35.0%
ATCAGAATCGTCACATCATG+AGG - Chr2:68839989-68840008 MsG0280010383.01.T01:intron 40.0%
CACATCATGAGGAATGTGAA+GGG - Chr2:68840000-68840019 MsG0280010383.01.T01:intron 40.0%
GAAGGCGAGAATTTCTACAA+TGG + Chr2:68838192-68838211 None:intergenic 40.0%
GAGAGAAACGAACTTGAAGT+TGG + Chr2:68838992-68839011 None:intergenic 40.0%
TCACATCATGAGGAATGTGA+AGG - Chr2:68839999-68840018 MsG0280010383.01.T01:intron 40.0%
ACCTGGACAATCCTTGATCA+GGG - Chr2:68837978-68837997 MsG0280010383.01.T01:CDS 45.0%
ATGTGTGTGTTTGAGCAGGA+TGG - Chr2:68839873-68839892 MsG0280010383.01.T01:intron 45.0%
ATTGTCCAGGTACTATTGCG+AGG + Chr2:68837969-68837988 None:intergenic 45.0%
GAATATGTCTCCCCTGATCA+AGG + Chr2:68837992-68838011 None:intergenic 45.0%
GAATGTGAAGGGACCAGTTA+GGG - Chr2:68840011-68840030 MsG0280010383.01.T01:CDS 45.0%
GAATTCCTCGCAATAGTACC+TGG - Chr2:68837961-68837980 MsG0280010383.01.T01:CDS 45.0%
GTGAAAGTTATGGGTCGTAC+TGG - Chr2:68839922-68839941 MsG0280010383.01.T01:intron 45.0%
GTGAATGTGTGTGTTTGAGC+AGG - Chr2:68839869-68839888 MsG0280010383.01.T01:intron 45.0%
GTTGTACTTCCTAGCTAGGA+AGG + Chr2:68838426-68838445 None:intergenic 45.0%
TACCTGGACAATCCTTGATC+AGG - Chr2:68837977-68837996 MsG0280010383.01.T01:CDS 45.0%
TGGATGGTAGGAGACAAGAA+TGG + Chr2:68838249-68838268 None:intergenic 45.0%
TGGTAGGAGACAAGAATGGT+TGG + Chr2:68838245-68838264 None:intergenic 45.0%
! CCTAGCTAGGAAGTACAACA+AGG - Chr2:68838427-68838446 MsG0280010383.01.T01:intron 45.0%
! CCTTGTTGTACTTCCTAGCT+AGG + Chr2:68838430-68838449 None:intergenic 45.0%
! GCTTCTTGTTATGTTGTCGG+TGG + Chr2:68838286-68838305 None:intergenic 45.0%
! TCAAGACCCTTTTCCCATGA+AGG - Chr2:68838713-68838732 MsG0280010383.01.T01:intron 45.0%
!! AAATAATGATTTATTAAATA+TGG + Chr2:68838587-68838606 None:intergenic 5.0%
!! AATAATGATTTATTAAATAT+GGG + Chr2:68838586-68838605 None:intergenic 5.0%
!! TCAAAATAAAAAAATTAATA+TGG + Chr2:68839085-68839104 None:intergenic 5.0%
CCTGGACAATCCTTGATCAG+GGG - Chr2:68837979-68837998 MsG0280010383.01.T01:CDS 50.0%
GGAATGTGAAGGGACCAGTT+AGG - Chr2:68840010-68840029 MsG0280010383.01.T01:CDS 50.0%
GGGGAGACATATTCCGACTT+TGG - Chr2:68837998-68838017 MsG0280010383.01.T01:CDS 50.0%
TACCATCCACCGTTTAGCAG+AGG - Chr2:68838260-68838279 MsG0280010383.01.T01:intron 50.0%
TATGGGTCGTACTGGATCCA+GGG - Chr2:68839930-68839949 MsG0280010383.01.T01:intron 50.0%
TCTGCTAAACGGTGGATGGT+AGG + Chr2:68838261-68838280 None:intergenic 50.0%
TTATGGGTCGTACTGGATCC+AGG - Chr2:68839929-68839948 MsG0280010383.01.T01:intron 50.0%
TTCTCTCTCAGACTCGAGGA+GGG + Chr2:68840052-68840071 MsG0280010382.01.T01:intron 50.0%
TTGCTTCTCTCTCAGACTCG+AGG + Chr2:68840056-68840075 MsG0280010382.01.T01:intron 50.0%
AGGATGTCTCCCTCCCTAAC+TGG + Chr2:68840027-68840046 MsG0280010382.01.T01:intron 55.0%
ATCCAGGGGACAAGTCACTC+AGG - Chr2:68839945-68839964 MsG0280010383.01.T01:intron 55.0%
ATGGGTCGTACTGGATCCAG+GGG - Chr2:68839931-68839950 MsG0280010383.01.T01:intron 55.0%
CCCCTGATCAAGGATTGTCC+AGG + Chr2:68837982-68838001 None:intergenic 55.0%
CTTCTCTCTCAGACTCGAGG+AGG + Chr2:68840053-68840072 MsG0280010382.01.T01:intron 55.0%
GCGAAGAAGCTAGCTGCGTA+TGG + Chr2:68838927-68838946 None:intergenic 55.0%
GTGAAGGGACCAGTTAGGGA+GGG - Chr2:68840015-68840034 MsG0280010383.01.T01:CDS 55.0%
TCTCAGACTCGAGGAGGGTA+AGG + Chr2:68840047-68840066 MsG0280010382.01.T01:intron 55.0%
TGTCGGTGGCCTCTGCTAAA+CGG + Chr2:68838272-68838291 None:intergenic 55.0%
TGTGAAGGGACCAGTTAGGG+AGG - Chr2:68840014-68840033 MsG0280010383.01.T01:CDS 55.0%
! AACCTGAGTGACTTGTCCCC+TGG + Chr2:68839950-68839969 MsG0280010382.01.T01:intron 55.0%
! GGAAAGTGCAGAATCGGCGT+GGG + Chr2:68838959-68838978 None:intergenic 55.0%
! TGGAAAGTGCAGAATCGGCG+TGG + Chr2:68838960-68838979 None:intergenic 55.0%
GGCCTCTGCTAAACGGTGGA+TGG + Chr2:68838265-68838284 None:intergenic 60.0%
CGGTGGCCTCTGCTAAACGG+TGG + Chr2:68838269-68838288 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr2 gene 68837940 68840089 68837940 ID=MsG0280010383.01;Name=MsG0280010383.01
Chr2 mRNA 68837940 68840089 68837940 ID=MsG0280010383.01.T01;Parent=MsG0280010383.01;Name=MsG0280010383.01.T01;_AED=0.50;_eAED=0.66;_QI=0|0|0|1|0|0|2|0|92
Chr2 exon 68840010 68840089 68840010 ID=MsG0280010383.01.T01:exon:14757;Parent=MsG0280010383.01.T01
Chr2 exon 68837940 68838138 68837940 ID=MsG0280010383.01.T01:exon:14756;Parent=MsG0280010383.01.T01
Chr2 CDS 68840010 68840089 68840010 ID=MsG0280010383.01.T01:cds;Parent=MsG0280010383.01.T01
Chr2 CDS 68837940 68838138 68837940 ID=MsG0280010383.01.T01:cds;Parent=MsG0280010383.01.T01
Gene Sequence

>MsG0280010383.01.T01

ATGAATACCATACAAAACATTGAATTCCTCGCAATAGTACCTGGACAATCCTTGATCAGGGGAGACATATTCCGACTTTGGATGGAATCACAGATTAAGCATGCTGTTGTTGTGAAAGTTATGGGTCGTACTGGATCCAGGGGACAAGTCACTCAGGTTAGAGTGAAGTTTTTGGATGATCAGAATCGTCACATCATGAGGAATGTGAAGGGACCAGTTAGGGAGGGAGACATCCTTACCCTCCTCGAGTCTGAGAGAGAAGCAAGAAGACTCCGATAG

Protein sequence

>MsG0280010383.01.T01

MNTIQNIEFLAIVPGQSLIRGDIFRLWMESQIKHAVVVKVMGRTGSRGQVTQVRVKFLDDQNRHIMRNVKGPVREGDILTLLESEREARRLR*