AlfalfaGEDB | MsG0180001829.01

M. sativa cultivar ZhongmuNo.1 / MsG0180001829.01

KEGG: K02985
GO: GO:0003723 GO:0003735 GO:0005840 GO:0006412 GO:0015935
PFAM: PF00189 PF07650

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180001829.01.T01	XP_003590123.1	100	239	0	0	1	239	1	239	3.13E-173	486
MsG0180001829.01.T02	XP_003590123.1	100	239	0	0	1	239	1	239	3.13E-173	486

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180001829.01.T01	Q9FJA6	88.841	233	26	0	1	233	1	233	2.07E-152	426
MsG0180001829.01.T02	Q9FJA6	88.841	233	26	0	1	233	1	233	2.07E-152	426

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180001829.01.T01	B7FH64	100.000	239	0	0	1	239	1	239	1.49e-173	486
MsG0180001829.01.T02	B7FH64	100.000	239	0	0	1	239	1	239	1.49e-173	486

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048933.01	MsG0180001829.01	0.826638	2.430306e-54	2.292224e-51
MsG0180000096.01	MsG0180001829.01	0.889274	2.805938e-73	2.248440e-69
MsG0180000118.01	MsG0180001829.01	0.923679	1.930828e-89	7.832390e-85
MsG0180001169.01	MsG0180001829.01	0.863589	2.234155e-64	6.808469e-61
MsG0180001829.01	MsG0180001837.01	0.845953	2.943288e-59	4.987594e-56
MsG0180001829.01	MsG0180002738.01	0.820263	7.523398e-53	5.924017e-50
MsG0180001829.01	MsG0180003585.01	0.823690	1.209338e-53	1.048871e-50
MsG0180001829.01	MsG0180003897.01	0.918848	9.381893e-87	2.919559e-82
MsG0180001829.01	MsG0180004064.01	0.850937	1.229146e-60	2.447625e-57
MsG0180001829.01	MsG0180004503.01	0.873571	1.326666e-67	5.784959e-64

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PPI

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Gene1	Gene2	Type
MsG0180000118.01	MsG0180001829.01	PPI
MsG0180000118.01	MsG0180001829.01	PPI
MsG0180001829.01	MsG0780037928.01	PPI
MsG0180001829.01	MsG0780037928.01	PPI
MsG0180001829.01	MsG0180001829.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180001829.01.T01	MTR_1g044660	100.000	239	0	0	1	239	1	239	3.79e-177	486
MsG0180001829.01.T01	MTR_1g023000	99.163	239	2	0	1	239	1	239	2.47e-176	484
MsG0180001829.01.T01	MTR_6g052140	94.538	238	8	1	1	238	1	233	6.77e-166	457
MsG0180001829.01.T01	MTR_6g052220	94.538	238	8	1	1	238	1	233	6.77e-166	457
MsG0180001829.01.T01	MTR_1g111450	86.383	235	31	1	1	234	1	235	5.29e-150	417
MsG0180001829.01.T02	MTR_1g044660	100.000	239	0	0	1	239	1	239	3.79e-177	486
MsG0180001829.01.T02	MTR_1g023000	99.163	239	2	0	1	239	1	239	2.47e-176	484
MsG0180001829.01.T02	MTR_6g052140	94.538	238	8	1	1	238	1	233	6.77e-166	457
MsG0180001829.01.T02	MTR_6g052220	94.538	238	8	1	1	238	1	233	6.77e-166	457
MsG0180001829.01.T02	MTR_1g111450	86.383	235	31	1	1	234	1	235	5.29e-150	417

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Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180001829.01.T01	AT5G35530	88.841	233	26	1	233	1	233	2.11e-153	426
MsG0180001829.01.T01	AT2G31610	88.987	227	25	1	227	1	227	4.58e-149	416
MsG0180001829.01.T01	AT3G53870	92.166	217	17	1	217	1	217	1.53e-148	414
MsG0180001829.01.T02	AT5G35530	88.841	233	26	1	233	1	233	2.11e-153	426
MsG0180001829.01.T02	AT2G31610	88.987	227	25	1	227	1	227	4.58e-149	416
MsG0180001829.01.T02	AT3G53870	92.166	217	17	1	217	1	217	1.53e-148	414

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Find 43 sgRNAs with CRISPR-Local

Find 260 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AATGAACATAAATATGTTAG+TGG	0.458859	1:-27839799	None:intergenic
AATGGTGACAATATCAGGGA+GGG	0.600476	1:-27839430	None:intergenic
ACCCTAGTTTCTTATGTGTG+CGG	0.602423	1:-27836956	None:intergenic
AGGAAGAGGAGGAGTACATC+AGG	0.468833	1:+27839463	MsG0180001829.01.T01:CDS
AGGTTAAGATCATGCTTGAC+TGG	0.451430	1:+27839376	MsG0180001829.01.T01:CDS
ATGAACATAAATATGTTAGT+GGG	0.544012	1:-27839798	None:intergenic
ATGCTTGACTGGGATCCTAA+AGG	0.556783	1:+27839387	MsG0180001829.01.T01:CDS
ATGGTGACAATATCAGGGAG+GGG	0.658335	1:-27839429	None:intergenic
ATGTACTCCTCCTCTTCCTT+TGG	0.368386	1:-27839459	None:intergenic
ATGTTTGCAGATTTAGCTAG+GGG	0.598120	1:-27837016	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAGAAATGGTAAAGTCACA+TGG	-	Chr1:27838492-27838511	None:intergenic	30.0%
AAATCCATGAAGTTTAAGGA+CGG	+	Chr1:27839141-27839160	MsG0180001829.01.T01:CDS	30.0%
AATATATCGGGTACAAAAAG+AGG	-	Chr1:27838822-27838841	None:intergenic	30.0%
AATATTGACGATTAAGAGAG+TGG	+	Chr1:27840719-27840738	MsG0180001829.01.T01:intron	30.0%
ACATAGCAAACATAAAACAC+AGG	-	Chr1:27838616-27838635	None:intergenic	30.0%
ATATATCGGGTACAAAAAGA+GGG	-	Chr1:27838821-27838840	None:intergenic	30.0%
CAAAACATTCTCAAACAACA+GGG	-	Chr1:27839533-27839552	None:intergenic	30.0%
CAACACATACTCATGTTAAA+TGG	+	Chr1:27838719-27838738	MsG0180001829.01.T01:intron	30.0%
CTGAAAGGAATATACAAACA+GGG	+	Chr1:27840272-27840291	MsG0180001829.01.T01:intron	30.0%
GAAACCCTAATTTCTTAGAA+AGG	+	Chr1:27840853-27840872	MsG0180001829.01.T01:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	27836941	27841025	27836941	ID=MsG0180001829.01;Name=MsG0180001829.01
Chr1	mRNA	27836941	27841025	27836941	ID=MsG0180001829.01.T01;Parent=MsG0180001829.01;Name=MsG0180001829.01.T01;_AED=0.50;_eAED=0.50;_QI=96\|1\|1\|1\|0.83\|0.71\|7\|219\|239
Chr1	exon	27836941	27837066	27836941	ID=MsG0180001829.01.T01:exon:1102;Parent=MsG0180001829.01.T01
Chr1	exon	27839360	27839722	27839360	ID=MsG0180001829.01.T01:exon:1107;Parent=MsG0180001829.01.T01
Chr1	exon	27840987	27841025	27840987	ID=MsG0180001829.01.T01:exon:1108;Parent=MsG0180001829.01.T01
Chr1	five_prime_UTR	27836941	27837036	27836941	ID=MsG0180001829.01.T01:five_prime_utr;Parent=MsG0180001829.01.T01
Chr1	CDS	27837037	27837066	27837037	ID=MsG0180001829.01.T01:cds;Parent=MsG0180001829.01.T01
Chr1	CDS	27837153	27837300	27837153	ID=MsG0180001829.01.T01:cds;Parent=MsG0180001829.01.T01
Chr1	CDS	27837795	27837966	27837795	ID=MsG0180001829.01.T01:cds;Parent=MsG0180001829.01.T01
Chr1	CDS	27838962	27839016	27838962	ID=MsG0180001829.01.T01:cds;Parent=MsG0180001829.01.T01
Chr1	CDS	27839105	27839236	27839105	ID=MsG0180001829.01.T01:cds;Parent=MsG0180001829.01.T01
Chr1	CDS	27839360	27839542	27839360	ID=MsG0180001829.01.T01:cds;Parent=MsG0180001829.01.T01
Chr1	three_prime_UTR	27839543	27839722	27839543	ID=MsG0180001829.01.T01:three_prime_utr;Parent=MsG0180001829.01.T01
Chr1	three_prime_UTR	27840987	27841025	27840987	ID=MsG0180001829.01.T01:three_prime_utr;Parent=MsG0180001829.01.T01
Chr1	mRNA	27836942	27839809	27836942	ID=MsG0180001829.01.T02;Parent=MsG0180001829.01;Name=MsG0180001829.01.T02;_AED=0.50;_eAED=0.50;_QI=95\|1\|1\|1\|1\|1\|6\|267\|239
Chr1	exon	27836942	27837066	27836942	ID=MsG0180001829.01.T02:exon:1109;Parent=MsG0180001829.01.T02
Chr1	exon	27839360	27839809	27839360	ID=MsG0180001829.01.T02:exon:1110;Parent=MsG0180001829.01.T02
Chr1	five_prime_UTR	27836942	27837036	27836942	ID=MsG0180001829.01.T02:five_prime_utr;Parent=MsG0180001829.01.T02
Chr1	CDS	27837037	27837066	27837037	ID=MsG0180001829.01.T02:cds;Parent=MsG0180001829.01.T02
Chr1	CDS	27837153	27837300	27837153	ID=MsG0180001829.01.T02:cds;Parent=MsG0180001829.01.T02
Chr1	CDS	27837795	27837966	27837795	ID=MsG0180001829.01.T02:cds;Parent=MsG0180001829.01.T02
Chr1	CDS	27838962	27839016	27838962	ID=MsG0180001829.01.T02:cds;Parent=MsG0180001829.01.T02
Chr1	CDS	27839105	27839236	27839105	ID=MsG0180001829.01.T02:cds;Parent=MsG0180001829.01.T02
Chr1	CDS	27839360	27839542	27839360	ID=MsG0180001829.01.T02:cds;Parent=MsG0180001829.01.T02
Chr1	three_prime_UTR	27839543	27839809	27839543	ID=MsG0180001829.01.T02:three_prime_utr;Parent=MsG0180001829.01.T02

Gene Sequence

>MsG0180001829.01.T01

ATGGCTACCCAAATGAGCAAAAAGCGAAAGTTCGTCGCTGACGGAGTTTTCTTCGCTGAATTGAATGAGGTTTTAACCAGAGAGCTTGCTGAAGATGGTTATTCTGGTGTTGAAGTTAGGGTTACCCCTATGCGCACTGAGATCATCATCAGGGCTACTCGTACCCAAGCTGTTCTCGGTGAAAAGGGAAGGAGAATAAGAGAGCTGACCTCAGTTGTCCAGAAGAGGTTCAAGTTTCCTGAGAACAGTGTTGAGCTTTACGCTGAGAAGGTCAACAACAGGGGACTTTGCGCCATTGCTCAGGCCGAGTCTCTTCGCTACAAGCTGCTCGGAGGTCTTGCTGTTCGCAGGGCCTGCTATGGTGTGTTGAGGTTTGTCATGGAAAGTGGTGCCAAGGGATGCGAGGTTATTGTCAGTGGAAAATTGAGGGCCCAGAGAGCCAAATCCATGAAGTTTAAGGACGGGTATATGATCTCGTCCGGACAACCTGTTAAGGATTACATTGACTCTGCTGTCAGACATGTTCTCCTTAGACAGGGTGTTCTCGGTATCAAGGTTAAGATCATGCTTGACTGGGATCCTAAAGGAAAGCAGGGCCCTAAAACTCCCCTCCCTGATATTGTCACCATTCACACTCCAAAGGAAGAGGAGGAGTACATCAGGCCTGCTGCTGTTGTGGCCAATGATATTGAGGTTCCTGTCCCAGTCCCTGTTGTTTGA

>MsG0180001829.01.T02

Protein sequence

>MsG0180001829.01.T01

MATQMSKKRKFVADGVFFAELNEVLTRELAEDGYSGVEVRVTPMRTEIIIRATRTQAVLGEKGRRIRELTSVVQKRFKFPENSVELYAEKVNNRGLCAIAQAESLRYKLLGGLAVRRACYGVLRFVMESGAKGCEVIVSGKLRAQRAKSMKFKDGYMISSGQPVKDYIDSAVRHVLLRQGVLGIKVKIMLDWDPKGKQGPKTPLPDIVTIHTPKEEEEYIRPAAVVANDIEVPVPVPVV*

>MsG0180001829.01.T02