AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280006629.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280006629.01.T01 MTR_2g012450 100.000 146 0 0 1 146 1 146 1.09e-103 293
MsG0280006629.01.T01 MTR_5g015570 95.205 146 7 0 1 146 1 146 2.12e-90 259
MsG0280006629.01.T01 MTR_5g096540 91.096 146 13 0 1 146 1 146 4.44e-87 251
MsG0280006629.01.T01 MTR_4g126220 88.235 68 8 0 13 80 103 170 6.77e-38 127
MsG0280006629.01.T01 MTR_8g073140 56.977 86 10 1 30 115 1 59 2.75e-23 87.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280006629.01.T01 AT5G67510 88.356 146 17 0 1 146 1 146 1.04e-92 265
MsG0280006629.01.T01 AT3G49910 85.616 146 21 0 1 146 1 146 1.23e-91 262

Find 51 sgRNAs with CRISPR-Local

Find 49 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATTATGCAGACCGTTGATT+AGG 0.363868 2:-4290238 None:intergenic
CATTGAACGGACGTTGTACT+TGG 0.377101 2:+4290539 None:intergenic
TCGCAAAGACGACGAAGTTC+AGG 0.379749 2:-4290511 MsG0280006629.01.T01:CDS
TTAGTGATAACAACCTTTGA+AGG 0.395167 2:+4290360 None:intergenic
ACTTCGTCGTCTTTGCGAAC+AGG 0.416424 2:+4290516 None:intergenic
GGGTCGTGCTGCTGCTGATA+AGG 0.420559 2:-4290289 MsG0280006629.01.T01:CDS
ACGGCTCTTCCTACGGCTGC+TGG 0.422834 2:+4290635 None:intergenic
ATGGATCCACCGTTAATGTT+GGG 0.429545 2:-4290389 MsG0280006629.01.T01:CDS
AAGACACGGCGGACGCTTGA+TGG 0.437229 2:+4290594 None:intergenic
AGGATTAACCCCAACATTAA+CGG 0.438217 2:+4290380 None:intergenic
CACTAAGCTCAGATTGGATA+AGG 0.438891 2:-4290343 MsG0280006629.01.T01:CDS
CCTGAGTAACCTTACCTTCA+CGG 0.446679 2:+4290466 None:intergenic
TGTTATCACTAAGCTCAGAT+TGG 0.466490 2:-4290349 MsG0280006629.01.T01:CDS
GACGAAGTTCAGGTCGTTCG+TGG 0.488796 2:-4290501 MsG0280006629.01.T01:CDS
ATTCAAAGGCCGTGAAGGTA+AGG 0.498862 2:-4290475 MsG0280006629.01.T01:CDS
CTCAGGTTTACCGTCGCAAG+TGG 0.512762 2:-4290449 MsG0280006629.01.T01:CDS
TGTTGGGGTTAATCCTTCAA+AGG 0.524932 2:-4290373 MsG0280006629.01.T01:CDS
GCTACTTGATCGTAAGGCTA+AGG 0.528270 2:-4290310 MsG0280006629.01.T01:CDS
CTACGGCTGCTGGAAACTCG+TGG 0.530535 2:+4290645 None:intergenic
AATGGATCCACCGTTAATGT+TGG 0.535095 2:-4290390 MsG0280006629.01.T01:CDS
TGCTGCTGCTGATAAGGAGA+AGG 0.537077 2:-4290283 MsG0280006629.01.T01:CDS
GAGCCGTAAGGCTCATTTCA+CGG 0.545137 2:-4290619 MsG0280006629.01.T01:CDS
ATCACAAGGGAGAAGGTGAA+TGG 0.545267 2:-4290408 MsG0280006629.01.T01:CDS
GAGCCTTACGGCTCTTCCTA+CGG 0.547173 2:+4290628 None:intergenic
CTACTTGATCGTAAGGCTAA+GGG 0.553374 2:-4290309 MsG0280006629.01.T01:CDS
GTCGTTCGTGGAACATTCAA+AGG 0.554383 2:-4290489 MsG0280006629.01.T01:CDS
TAAATCGCTACTTGATCGTA+AGG 0.555125 2:-4290316 MsG0280006629.01.T01:CDS
GGGTACTAAGTTTGCTCCTG+AGG 0.563912 2:-4290262 MsG0280006629.01.T01:CDS
AACGGACGTTGTACTTGGAT+CGG 0.564652 2:+4290544 None:intergenic
CCGTGAAGGTAAGGTTACTC+AGG 0.566699 2:-4290466 MsG0280006629.01.T01:CDS
CTTTGCGAACAGGCATTGAA+CGG 0.570326 2:+4290526 None:intergenic
TGGATCCACCGTTAATGTTG+GGG 0.571824 2:-4290388 MsG0280006629.01.T01:CDS
GTTGTACTTGGATCGGAGAT+CGG 0.572814 2:+4290551 None:intergenic
ACGGTCTGCATAATATCCTC+AGG 0.575139 2:+4290246 None:intergenic
GCGCCGTGAAATGAGCCTTA+CGG 0.575259 2:+4290616 None:intergenic
CGAACGCATCACAAGGGAGA+AGG 0.580140 2:-4290415 MsG0280006629.01.T01:CDS
GATCGGAGATCGGCGGAGAG+TGG 0.584970 2:+4290561 None:intergenic
TACGGCTGCTGGAAACTCGT+GGG 0.586325 2:+4290646 None:intergenic
TTCACATCGAACGCATCACA+AGG 0.588472 2:-4290422 MsG0280006629.01.T01:CDS
CAGCCGTAGGAAGAGCCGTA+AGG 0.591177 2:-4290631 MsG0280006629.01.T01:CDS
GGAACATTCAAAGGCCGTGA+AGG 0.606062 2:-4290480 MsG0280006629.01.T01:CDS
GATTCAAGAATCCTAATCAA+CGG 0.625900 2:+4290227 None:intergenic
GCTGCTGCTGATAAGGAGAA+GGG 0.634378 2:-4290282 MsG0280006629.01.T01:CDS
TGTGAATCACCCACTTGCGA+CGG 0.658219 2:+4290439 None:intergenic
TCAGGTTTACCGTCGCAAGT+GGG 0.670860 2:-4290448 MsG0280006629.01.T01:CDS
ATTAACCCCAACATTAACGG+TGG 0.685240 2:+4290383 None:intergenic
CACGAGTTTCCAGCAGCCGT+AGG 0.686122 2:-4290644 MsG0280006629.01.T01:CDS
GTGGCGCGCTCATTAAGACA+CGG 0.705562 2:+4290580 None:intergenic
GTACTTGGATCGGAGATCGG+CGG 0.709980 2:+4290554 None:intergenic
TCACATCGAACGCATCACAA+GGG 0.710980 2:-4290421 MsG0280006629.01.T01:CDS
GCGCGCTCATTAAGACACGG+CGG 0.823768 2:+4290583 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
TTAGTGATAACAACCTTTGA+AGG + Chr2:4290539-4290558 None:intergenic 30.0%
AGGATTAACCCCAACATTAA+CGG + Chr2:4290519-4290538 None:intergenic 35.0%
TAAATCGCTACTTGATCGTA+AGG - Chr2:4290580-4290599 MsG0280006629.01.T01:CDS 35.0%
TGTTATCACTAAGCTCAGAT+TGG - Chr2:4290547-4290566 MsG0280006629.01.T01:CDS 35.0%
AATGGATCCACCGTTAATGT+TGG - Chr2:4290506-4290525 MsG0280006629.01.T01:CDS 40.0%
ATGGATCCACCGTTAATGTT+GGG - Chr2:4290507-4290526 MsG0280006629.01.T01:CDS 40.0%
ATTAACCCCAACATTAACGG+TGG + Chr2:4290516-4290535 None:intergenic 40.0%
CACTAAGCTCAGATTGGATA+AGG - Chr2:4290553-4290572 MsG0280006629.01.T01:CDS 40.0%
CTACTTGATCGTAAGGCTAA+GGG - Chr2:4290587-4290606 MsG0280006629.01.T01:CDS 40.0%
TGTTGGGGTTAATCCTTCAA+AGG - Chr2:4290523-4290542 MsG0280006629.01.T01:CDS 40.0%
AACGGACGTTGTACTTGGAT+CGG + Chr2:4290355-4290374 None:intergenic 45.0%
ACGGTCTGCATAATATCCTC+AGG + Chr2:4290653-4290672 None:intergenic 45.0%
ATCACAAGGGAGAAGGTGAA+TGG - Chr2:4290488-4290507 MsG0280006629.01.T01:CDS 45.0%
ATTCAAAGGCCGTGAAGGTA+AGG - Chr2:4290421-4290440 MsG0280006629.01.T01:CDS 45.0%
CATTGAACGGACGTTGTACT+TGG + Chr2:4290360-4290379 None:intergenic 45.0%
CCTGAGTAACCTTACCTTCA+CGG + Chr2:4290433-4290452 None:intergenic 45.0%
GCTACTTGATCGTAAGGCTA+AGG - Chr2:4290586-4290605 MsG0280006629.01.T01:CDS 45.0%
GTCGTTCGTGGAACATTCAA+AGG - Chr2:4290407-4290426 MsG0280006629.01.T01:CDS 45.0%
GTTGTACTTGGATCGGAGAT+CGG + Chr2:4290348-4290367 None:intergenic 45.0%
TCACATCGAACGCATCACAA+GGG - Chr2:4290475-4290494 MsG0280006629.01.T01:CDS 45.0%
TGGATCCACCGTTAATGTTG+GGG - Chr2:4290508-4290527 MsG0280006629.01.T01:CDS 45.0%
TTCACATCGAACGCATCACA+AGG - Chr2:4290474-4290493 MsG0280006629.01.T01:CDS 45.0%
!! CTTTGCGAACAGGCATTGAA+CGG + Chr2:4290373-4290392 None:intergenic 45.0%
ACTTCGTCGTCTTTGCGAAC+AGG + Chr2:4290383-4290402 None:intergenic 50.0%
CCGTGAAGGTAAGGTTACTC+AGG - Chr2:4290430-4290449 MsG0280006629.01.T01:CDS 50.0%
GAGCCGTAAGGCTCATTTCA+CGG - Chr2:4290277-4290296 MsG0280006629.01.T01:CDS 50.0%
GCTGCTGCTGATAAGGAGAA+GGG - Chr2:4290614-4290633 MsG0280006629.01.T01:CDS 50.0%
GGAACATTCAAAGGCCGTGA+AGG - Chr2:4290416-4290435 MsG0280006629.01.T01:CDS 50.0%
TCAGGTTTACCGTCGCAAGT+GGG - Chr2:4290448-4290467 MsG0280006629.01.T01:CDS 50.0%
TCGCAAAGACGACGAAGTTC+AGG - Chr2:4290385-4290404 MsG0280006629.01.T01:CDS 50.0%
TGCTGCTGCTGATAAGGAGA+AGG - Chr2:4290613-4290632 MsG0280006629.01.T01:CDS 50.0%
TGTGAATCACCCACTTGCGA+CGG + Chr2:4290460-4290479 None:intergenic 50.0%
! GGGTACTAAGTTTGCTCCTG+AGG - Chr2:4290634-4290653 MsG0280006629.01.T01:CDS 50.0%
CGAACGCATCACAAGGGAGA+AGG - Chr2:4290481-4290500 MsG0280006629.01.T01:CDS 55.0%
CTCAGGTTTACCGTCGCAAG+TGG - Chr2:4290447-4290466 MsG0280006629.01.T01:CDS 55.0%
GACGAAGTTCAGGTCGTTCG+TGG - Chr2:4290395-4290414 MsG0280006629.01.T01:CDS 55.0%
GAGCCTTACGGCTCTTCCTA+CGG + Chr2:4290271-4290290 None:intergenic 55.0%
GCGCCGTGAAATGAGCCTTA+CGG + Chr2:4290283-4290302 None:intergenic 55.0%
GTACTTGGATCGGAGATCGG+CGG + Chr2:4290345-4290364 None:intergenic 55.0%
GTGGCGCGCTCATTAAGACA+CGG + Chr2:4290319-4290338 None:intergenic 55.0%
TACGGCTGCTGGAAACTCGT+GGG + Chr2:4290253-4290272 None:intergenic 55.0%
AAGACACGGCGGACGCTTGA+TGG + Chr2:4290305-4290324 None:intergenic 60.0%
CACGAGTTTCCAGCAGCCGT+AGG - Chr2:4290252-4290271 MsG0280006629.01.T01:CDS 60.0%
CAGCCGTAGGAAGAGCCGTA+AGG - Chr2:4290265-4290284 MsG0280006629.01.T01:CDS 60.0%
CTACGGCTGCTGGAAACTCG+TGG + Chr2:4290254-4290273 None:intergenic 60.0%
GCGCGCTCATTAAGACACGG+CGG + Chr2:4290316-4290335 None:intergenic 60.0%
! GGGTCGTGCTGCTGCTGATA+AGG - Chr2:4290607-4290626 MsG0280006629.01.T01:CDS 60.0%
ACGGCTCTTCCTACGGCTGC+TGG + Chr2:4290264-4290283 None:intergenic 65.0%
GATCGGAGATCGGCGGAGAG+TGG + Chr2:4290338-4290357 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr2 gene 4290239 4290679 4290239 ID=MsG0280006629.01;Name=MsG0280006629.01
Chr2 mRNA 4290239 4290679 4290239 ID=MsG0280006629.01.T01;Parent=MsG0280006629.01;Name=MsG0280006629.01.T01;_AED=0.47;_eAED=0.47;_QI=0|-1|0|1|-1|1|1|0|146
Chr2 exon 4290239 4290679 4290239 ID=MsG0280006629.01.T01:exon:1034;Parent=MsG0280006629.01.T01
Chr2 CDS 4290239 4290679 4290239 ID=MsG0280006629.01.T01:cds;Parent=MsG0280006629.01.T01
Gene Sequence

>MsG0280006629.01.T01

ATGAAGTTCAACCCACGAGTTTCCAGCAGCCGTAGGAAGAGCCGTAAGGCTCATTTCACGGCGCCATCAAGCGTCCGCCGTGTCTTAATGAGCGCGCCACTCTCCGCCGATCTCCGATCCAAGTACAACGTCCGTTCAATGCCTGTTCGCAAAGACGACGAAGTTCAGGTCGTTCGTGGAACATTCAAAGGCCGTGAAGGTAAGGTTACTCAGGTTTACCGTCGCAAGTGGGTGATTCACATCGAACGCATCACAAGGGAGAAGGTGAATGGATCCACCGTTAATGTTGGGGTTAATCCTTCAAAGGTTGTTATCACTAAGCTCAGATTGGATAAGGATCGTAAATCGCTACTTGATCGTAAGGCTAAGGGTCGTGCTGCTGCTGATAAGGAGAAGGGTACTAAGTTTGCTCCTGAGGATATTATGCAGACCGTTGATTAG

Protein sequence

>MsG0280006629.01.T01

MKFNPRVSSSRRKSRKAHFTAPSSVRRVLMSAPLSADLRSKYNVRSMPVRKDDEVQVVRGTFKGREGKVTQVYRRKWVIHIERITREKVNGSTVNVGVNPSKVVITKLRLDKDRKSLLDRKAKGRAAADKEKGTKFAPEDIMQTVD*