AlfalfaGEDB | MsG0880045155.01

M. sativa cultivar ZhongmuNo.1 / MsG0880045155.01

KEGG: K09658
GO: GO:0019348 GO:0030176 GO:0030234
PFAM: PF07297

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880045155.01.T01	XP_003628775.1	100	66	0	0	35	100	15	80	4.96E-39	135

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880045155.01.T01	Q9CA79	80.328	61	12	0	40	100	20	80	1.21E-31	109

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880045155.01.T01	G7LH02	100.000	66	0	0	35	100	15	80	2.37e-39	135

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180000470.01	MsG0880045155.01	-0.804234	2.381698e-49	1.221541e-46
MsG0180000650.01	MsG0880045155.01	-0.800348	1.503217e-48	6.983579e-46
MsG0180001088.01	MsG0880045155.01	-0.805109	1.564328e-49	8.206464e-47
MsG0880043937.01	MsG0880045155.01	-0.813560	2.406185e-51	1.576534e-48
MsG0880045155.01	MsG0880045371.01	-0.800744	1.248446e-48	5.858260e-46
MsG0880045155.01	MsG0880045992.01	-0.810508	1.113454e-50	6.724133e-48
MsG0180004765.01	MsG0880045155.01	-0.800703	1.272673e-48	5.965594e-46
MsG0180006054.01	MsG0880045155.01	-0.840331	9.273200e-58	1.316425e-54
MsG0280006309.01	MsG0880045155.01	-0.815459	9.144074e-52	6.308318e-49
MsG0780041468.01	MsG0880045155.01	-0.811777	5.909128e-51	3.690630e-48
MsG0780041747.01	MsG0880045155.01	-0.805491	1.301055e-49	6.892686e-47
MsG0880042161.01	MsG0880045155.01	-0.809217	2.109897e-50	1.231552e-47
MsG0880043157.01	MsG0880045155.01	-0.822637	2.129485e-53	1.792627e-50
MsG0280010807.01	MsG0880045155.01	-0.813183	2.911782e-51	1.888236e-48
MsG0280010916.01	MsG0880045155.01	-0.810263	1.257482e-50	7.544939e-48
MsG0280011272.01	MsG0880045155.01	0.805510	1.289507e-49	6.834410e-47
MsG0280011404.01	MsG0880045155.01	-0.818080	2.361618e-52	1.750321e-49
MsG0380012005.01	MsG0880045155.01	-0.826827	2.191061e-54	2.077744e-51
MsG0380012085.01	MsG0880045155.01	-0.828579	8.311713e-55	8.295478e-52
MsG0380012365.01	MsG0880045155.01	-0.805909	1.063021e-49	5.692530e-47
MsG0380012567.01	MsG0880045155.01	-0.800690	1.280618e-48	6.000987e-46
MsG0380014727.01	MsG0880045155.01	-0.814322	1.634291e-51	1.093177e-48
MsG0380014866.01	MsG0880045155.01	-0.807003	6.248377e-50	3.441100e-47
MsG0680030880.01	MsG0880045155.01	-0.805699	1.176732e-49	6.267824e-47
MsG0680031998.01	MsG0880045155.01	-0.806796	6.912799e-50	3.786645e-47
MsG0680032460.01	MsG0880045155.01	0.832952	7.047110e-56	7.998362e-53
MsG0680033465.01	MsG0880045155.01	-0.858745	6.672875e-63	1.720707e-59
MsG0680034721.01	MsG0880045155.01	-0.803274	3.769149e-49	1.886112e-46
MsG0480018653.01	MsG0880045155.01	-0.827239	1.746031e-54	1.675945e-51
MsG0480019711.01	MsG0880045155.01	-0.815617	8.434660e-52	5.844511e-49
MsG0480020024.01	MsG0880045155.01	-0.825683	4.102075e-54	3.765190e-51
MsG0480020935.01	MsG0880045155.01	0.822398	2.420442e-53	2.023721e-50
MsG0480021561.01	MsG0880045155.01	-0.806220	9.144120e-50	4.935837e-47
MsG0480021869.01	MsG0880045155.01	-0.806083	9.773089e-50	5.256698e-47
MsG0480022259.01	MsG0880045155.01	-0.800628	1.318278e-48	6.167945e-46
MsG0480022359.01	MsG0880045155.01	-0.814426	1.550006e-51	1.039727e-48
MsG0480022802.01	MsG0880045155.01	-0.825784	3.880093e-54	3.571924e-51
MsG0480023251.01	MsG0880045155.01	-0.829708	4.426441e-55	4.566566e-52
MsG0580024363.01	MsG0880045155.01	0.820209	7.741471e-53	6.086693e-50
MsG0580024790.01	MsG0880045155.01	0.855719	5.223248e-62	1.217248e-58
MsG0580025059.01	MsG0880045155.01	-0.813893	2.032887e-51	1.344043e-48
MsG0580025207.01	MsG0880045155.01	-0.803953	2.726029e-49	1.388205e-46
MsG0580025407.01	MsG0880045155.01	0.822509	2.281099e-53	1.913170e-50
MsG0580025832.01	MsG0880045155.01	-0.807883	4.065877e-50	2.290952e-47
MsG0580025987.01	MsG0880045155.01	-0.800710	1.268176e-48	5.945667e-46
MsG0380015891.01	MsG0880045155.01	-0.823696	1.205409e-53	1.045678e-50
MsG0380017202.01	MsG0880045155.01	-0.853715	1.989001e-61	4.337392e-58
MsG0380017293.01	MsG0880045155.01	-0.800578	1.349831e-48	6.307452e-46
MsG0380017437.01	MsG0880045155.01	0.832334	1.003321e-55	1.117820e-52
MsG0380017931.01	MsG0880045155.01	-0.817475	3.234873e-52	2.358324e-49
MsG0580026122.01	MsG0880045155.01	-0.826461	2.679212e-54	2.514213e-51
MsG0580028428.01	MsG0880045155.01	-0.810705	1.009366e-50	6.127908e-48
MsG0580029472.01	MsG0880045155.01	-0.807156	5.801099e-50	3.207464e-47
MsG0580029880.01	MsG0880045155.01	-0.824095	9.715503e-54	8.521715e-51
MsG0280006601.01	MsG0880045155.01	-0.816478	5.415769e-52	3.841671e-49
MsG0280006753.01	MsG0880045155.01	0.808896	2.471925e-50	1.430396e-47
MsG0280008894.01	MsG0880045155.01	-0.800621	1.322589e-48	6.186985e-46
MsG0680035707.01	MsG0880045155.01	-0.810855	9.367187e-51	5.709826e-48
MsG0780036593.01	MsG0880045155.01	-0.803541	3.318293e-49	1.672026e-46
MsG0780036596.01	MsG0880045155.01	-0.803889	2.809746e-49	1.428522e-46
MsG0780039438.01	MsG0880045155.01	-0.804157	2.472104e-49	1.265454e-46
MsG0780039867.01	MsG0880045155.01	0.821943	3.085624e-53	2.546383e-50

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880045155.01.T01	MTR_8g066600	100.000	66	0	0	35	100	15	80	6.00e-43	135
MsG0880045155.01.T01	MTR_2g014320	100.000	64	0	0	35	98	15	78	1.44e-41	131

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0880045155.01.T01	AT1G74340	80.328	61	12	0	40	100	20	80	1.23e-32	109
MsG0880045155.01.T01	AT1G74340	80.328	61	12	0	40	100	20	80	1.23e-32	109

Find 17 sgRNAs with CRISPR-Local

Find 24 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TATTCACTTATTATACTTTC+TGG	0.197235	8:+55009281	MsG0880045155.01.T01:CDS
TGCCTCTTATGTATATTTAT+TGG	0.200273	8:+55009494	MsG0880045155.01.T01:CDS
ACATTATGCCTTCTTCTTCT+TGG	0.225894	8:-55009534	None:intergenic
GCCTCTTATGTATATTTATT+GGG	0.258483	8:+55009495	MsG0880045155.01.T01:CDS
TATTAATATGGCATATTCTT+GGG	0.311096	8:-55009444	None:intergenic
GTATTAATATGGCATATTCT+TGG	0.347420	8:-55009445	None:intergenic
AGCAGAAACAGGTATTAATA+TGG	0.371224	8:-55009456	None:intergenic
TACTTTCTGGGTTATCATCC+TGG	0.412601	8:+55009294	MsG0880045155.01.T01:CDS
ATTCACTTATTATACTTTCT+GGG	0.420687	8:+55009282	MsG0880045155.01.T01:CDS
ACCCAATAAATATACATAAG+AGG	0.435006	8:-55009496	None:intergenic
AGAGCAACACCAGCAGAAAC+AGG	0.472824	8:-55009467	None:intergenic
ATATTAATACCTGTTTCTGC+TGG	0.492249	8:+55009458	MsG0880045155.01.T01:CDS
GAACTAGCAGACAGAGCAGT+TGG	0.505593	8:+55009230	MsG0880045155.01.T01:CDS
AAATGATCATCATCTACAAA+GGG	0.512690	8:-55009401	None:intergenic
ATTAATATGGCATATTCTTG+GGG	0.556687	8:-55009443	None:intergenic
GCTCAAATCCAAGAAGAAGA+AGG	0.579376	8:+55009526	MsG0880045155.01.T01:CDS
GAAATGATCATCATCTACAA+AGG	0.583886	8:-55009402	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	CAAGTATTTTAAATGTATTA+TGG	+	Chr8:55009329-55009348	MsG0880045155.01.T01:intron	15.0%
!!!	CATAATACATTTAAAATACT+TGG	-	Chr8:55009331-55009350	None:intergenic	15.0%
!!	ACAATATCGAAAAACTTATT+AGG	-	Chr8:55009371-55009390	None:intergenic	20.0%
!!	ACTTATTAGGAAGTTAAATA+AGG	-	Chr8:55009358-55009377	None:intergenic	20.0%
!!	ATTCACTTATTATACTTTCT+GGG	+	Chr8:55009282-55009301	MsG0880045155.01.T01:CDS	20.0%
!!	TATTAATATGGCATATTCTT+GGG	-	Chr8:55009447-55009466	None:intergenic	20.0%
!!	TATTCACTTATTATACTTTC+TGG	+	Chr8:55009281-55009300	MsG0880045155.01.T01:CDS	20.0%
!	AAAAACGATAGGAATCAAAA+TGG	+	Chr8:55009208-55009227	MsG0880045155.01.T01:CDS	25.0%
!	AAATGATCATCATCTACAAA+GGG	-	Chr8:55009404-55009423	None:intergenic	25.0%
!	ACCCAATAAATATACATAAG+AGG	-	Chr8:55009499-55009518	None:intergenic	25.0%
!	ATTAATATGGCATATTCTTG+GGG	-	Chr8:55009446-55009465	None:intergenic	25.0%
!	GCCTCTTATGTATATTTATT+GGG	+	Chr8:55009495-55009514	MsG0880045155.01.T01:CDS	25.0%
!	GTATTAATATGGCATATTCT+TGG	-	Chr8:55009448-55009467	None:intergenic	25.0%
!	TGCCTCTTATGTATATTTAT+TGG	+	Chr8:55009494-55009513	MsG0880045155.01.T01:CDS	25.0%
	AGCAGAAACAGGTATTAATA+TGG	-	Chr8:55009459-55009478	None:intergenic	30.0%
	GAAATGATCATCATCTACAA+AGG	-	Chr8:55009405-55009424	None:intergenic	30.0%
!!	ATATTAATACCTGTTTCTGC+TGG	+	Chr8:55009458-55009477	MsG0880045155.01.T01:CDS	30.0%
!!!	TGTGTATTTTTGTTGTTGAC+AGG	+	Chr8:55009181-55009200	MsG0880045155.01.T01:CDS	30.0%
	GCTCAAATCCAAGAAGAAGA+AGG	+	Chr8:55009526-55009545	MsG0880045155.01.T01:CDS	40.0%
	TACTTGGCATGTTACTAACC+AGG	-	Chr8:55009315-55009334	None:intergenic	40.0%
	TACTTTCTGGGTTATCATCC+TGG	+	Chr8:55009294-55009313	MsG0880045155.01.T01:CDS	40.0%
	TGACAGGAAGCAAAAACGAT+AGG	+	Chr8:55009197-55009216	MsG0880045155.01.T01:CDS	40.0%
	AGAGCAACACCAGCAGAAAC+AGG	-	Chr8:55009470-55009489	None:intergenic	50.0%
	GAACTAGCAGACAGAGCAGT+TGG	+	Chr8:55009230-55009249	MsG0880045155.01.T01:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
Chr8	gene	55009166	55009553	55009166	ID=MsG0880045155.01;Name=MsG0880045155.01
Chr8	mRNA	55009166	55009553	55009166	ID=MsG0880045155.01.T01;Parent=MsG0880045155.01;Name=MsG0880045155.01.T01;_AED=0.47;_eAED=0.62;_QI=0\|0\|0\|1\|0\|0\|2\|0\|100
Chr8	exon	55009166	55009315	55009166	ID=MsG0880045155.01.T01:exon:23784;Parent=MsG0880045155.01.T01
Chr8	exon	55009401	55009553	55009401	ID=MsG0880045155.01.T01:exon:23785;Parent=MsG0880045155.01.T01
Chr8	CDS	55009166	55009315	55009166	ID=MsG0880045155.01.T01:cds;Parent=MsG0880045155.01.T01
Chr8	CDS	55009401	55009553	55009401	ID=MsG0880045155.01.T01:cds;Parent=MsG0880045155.01.T01
Chr8	mRNA	55009411	55009464	55009411	ID=MsG0880045155.01.T02;Parent=MsG0880045155.01;Name=MsG0880045155.01.T02;_AED=0.49;_eAED=0.49;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|17
Chr8	exon	55009411	55009464	55009411	ID=MsG0880045155.01.T02:exon:23786;Parent=MsG0880045155.01.T02
Chr8	CDS	55009411	55009464	55009411	ID=MsG0880045155.01.T02:cds;Parent=MsG0880045155.01.T02

Gene Sequence

>MsG0880045155.01.T01

ATGCTAACAGTCATCTGTGTATTTTTGTTGTTGACAGGAAGCAAAAACGATAGGAATCAAAATGGAACTAGCAGACAGAGCAGTTGGATTTCTATTGTCTGTATAAGCTTATCGATATTCACTTATTATACTTTCTGGGTTATCATCCTGCCCTTTGTAGATGATGATCATTTCGTACACAAGTATTTCTTACCCCAAGAATATGCCATATTAATACCTGTTTCTGCTGGTGTTGCTCTTATTTGCCTCTTATGTATATTTATTGGGTTCGTGATGCTCAAATCCAAGAAGAAGAAGGCATAA

>MsG0880045155.01.T02

ATGATGATCATTTCGTACACAAGTATTTCTTACCCCAAGAATATGCCATATTAA

Protein sequence

>MsG0880045155.01.T01

MLTVICVFLLLTGSKNDRNQNGTSRQSSWISIVCISLSIFTYYTFWVIILPFVDDDHFVHKYFLPQEYAILIPVSAGVALICLLCIFIGFVMLKSKKKKA*

>MsG0880045155.01.T02

MMIISYTSISYPKNMPY*