AlfalfaGEDB | MsG0580024412.01

M. sativa cultivar ZhongmuNo.1 / MsG0580024412.01

KEGG: -
GO: GO:0005506 GO:0016705 GO:0020037 GO:0055114
PFAM: PF00067

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024412.01.T01	KEH27443.1	97.959	147	3	0	1	147	1	147	8.31E-101	314
MsG0580024412.01.T01	KEH27443.1	61.864	118	33	3	169	286	337	442	1.03E-30	131

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024412.01.T01	Q7XU38	63.38	142	52	0	17	158	57	198	7.17E-65	216

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024412.01.T01	A0A072UED4	97.959	147	3	0	1	147	1	147	3.97e-101	314

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180000147.01	MsG0580024412.01	0.816063	6.708051e-52	4.704914e-49
MsG0180000148.01	MsG0580024412.01	0.837284	5.690456e-57	7.356435e-54
MsG0180000459.01	MsG0580024412.01	-0.810404	1.172297e-50	7.059820e-48
MsG0180000466.01	MsG0580024412.01	0.806519	7.909121e-50	4.301766e-47
MsG0180001029.01	MsG0580024412.01	0.819720	1.001590e-52	7.768520e-50
MsG0180001513.01	MsG0580024412.01	0.800121	1.672166e-48	7.723607e-46
MsG0180002238.01	MsG0580024412.01	0.807142	5.840399e-50	3.227980e-47
MsG0180004626.01	MsG0580024412.01	0.805733	1.157567e-49	6.171248e-47
MsG0180005648.01	MsG0580024412.01	0.809762	1.612093e-50	9.547434e-48
MsG0180005681.01	MsG0580024412.01	0.801241	9.882438e-49	4.696198e-46
MsG0280011238.01	MsG0580024412.01	0.809297	2.028310e-50	1.186449e-47
MsG0280011267.01	MsG0580024412.01	0.809305	2.020875e-50	1.182313e-47
MsG0280011292.01	MsG0580024412.01	0.802985	4.325576e-49	2.148527e-46
MsG0380011702.01	MsG0580024412.01	0.802849	4.615549e-49	2.284812e-46
MsG0480019171.01	MsG0580024412.01	0.801392	9.199578e-49	4.388245e-46
MsG0480019217.01	MsG0580024412.01	0.851825	6.894144e-61	1.412751e-57
MsG0480020810.01	MsG0580024412.01	0.802371	5.790337e-49	2.831742e-46
MsG0480020904.01	MsG0580024412.01	0.813430	2.569762e-51	1.677807e-48
MsG0480022055.01	MsG0580024412.01	0.803956	2.721755e-49	1.386137e-46
MsG0480022308.01	MsG0580024412.01	0.823784	1.149616e-53	9.996929e-51
MsG0580024087.01	MsG0580024412.01	0.812428	4.261637e-51	2.709020e-48
MsG0580024220.01	MsG0580024412.01	0.817265	3.606254e-52	2.613732e-49
MsG0580024412.01	MsG0580026019.01	0.811894	5.572866e-51	3.491306e-48
MsG0580024412.01	MsG0580028041.01	0.800860	1.181876e-48	5.562626e-46
MsG0580024412.01	MsG0580028490.01	0.813069	3.085165e-51	1.994520e-48
MsG0580024412.01	MsG0680030296.01	0.824677	7.091080e-54	6.325198e-51
MsG0580024412.01	MsG0680030613.01	0.854764	9.906322e-62	2.236298e-58
MsG0580024412.01	MsG0680032932.01	0.826644	2.423133e-54	2.285795e-51
MsG0580024412.01	MsG0780036661.01	0.803009	4.276756e-49	2.125522e-46
MsG0580024412.01	MsG0780039237.01	0.803327	3.675501e-49	1.841715e-46
MsG0580024412.01	MsG0780040716.01	0.807626	4.611661e-50	2.581152e-47
MsG0580024412.01	MsG0780040783.01	0.808736	2.674772e-50	1.541219e-47
MsG0580024412.01	MsG0880042591.01	0.836925	7.032593e-57	8.993104e-54
MsG0580024412.01	MsG0880043669.01	0.808443	3.089372e-50	1.766754e-47
MsG0580024412.01	MsG0880043998.01	0.813019	3.162820e-51	2.042061e-48
MsG0380016300.01	MsG0580024412.01	0.804148	2.482103e-49	1.270308e-46
MsG0380016718.01	MsG0580024412.01	-0.800048	1.730154e-48	7.976637e-46
MsG0380017168.01	MsG0580024412.01	0.819610	1.061237e-52	8.205340e-50
MsG0380017901.01	MsG0580024412.01	0.822441	2.365558e-53	1.980209e-50
MsG0380018006.01	MsG0580024412.01	0.803589	3.243020e-49	1.636128e-46
MsG0480018433.01	MsG0580024412.01	0.820095	8.218347e-53	6.442121e-50
MsG0280007505.01	MsG0580024412.01	0.814071	1.856992e-51	1.233696e-48
MsG0280007906.01	MsG0580024412.01	0.812743	3.636592e-51	2.330852e-48
MsG0280007911.01	MsG0580024412.01	0.827595	1.434511e-54	1.391057e-51
MsG0280009526.01	MsG0580024412.01	0.811988	5.314159e-51	3.337911e-48

PPI

Gene1	Gene2	Type
MsG0280006772.01	MsG0580024412.01	PPI
MsG0580024412.01	MsG0380015456.01	PPI
MsG0580025493.01	MsG0580024412.01	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024412.01.T01	MTR_5g010750	97.959	147	3	0	1	147	1	147	1.01e-104	314
MsG0580024412.01.T01	MTR_5g010750	61.864	118	33	3	169	286	337	442	1.25e-34	131
MsG0580024412.01.T01	MTR_5g010750	97.959	147	3	0	1	147	1	147	6.71e-104	313
MsG0580024412.01.T01	MTR_5g010750	71.698	159	33	3	169	327	337	483	6.69e-68	220
MsG0580024412.01.T01	MTR_5g020020	33.333	144	96	0	6	149	11	154	1.33e-23	101
MsG0580024412.01.T01	MTR_5g020020	30.556	144	86	4	170	311	346	477	5.69e-12	66.6
MsG0580024412.01.T01	MTR_6g069000	36.029	136	87	0	29	164	48	183	1.18e-22	98.2
MsG0580024412.01.T01	MTR_6g069000	36.029	136	87	0	29	164	38	173	1.22e-22	98.2
MsG0580024412.01.T01	MTR_8g077810	37.500	128	80	0	31	158	33	160	1.43e-22	98.6
MsG0580024412.01.T01	MTR_8g077810	31.724	145	86	4	170	311	343	477	2.31e-14	73.9
MsG0580024412.01.T01	MTR_5g082520	34.711	121	79	0	30	150	27	147	2.88e-22	97.4
MsG0580024412.01.T01	MTR_5g082520	30.872	149	92	2	170	318	332	469	2.68e-14	73.9
MsG0580024412.01.T01	MTR_3g070380	34.711	121	79	0	30	150	28	148	1.03e-21	95.9
MsG0580024412.01.T01	MTR_3g070380	28.571	147	96	3	170	316	333	470	1.89e-11	65.1
MsG0580024412.01.T01	MTR_3g007320	32.394	142	89	3	5	145	7	142	4.24e-21	94.0
MsG0580024412.01.T01	MTR_3g007320	27.442	215	112	9	113	312	277	462	1.65e-14	74.3
MsG0580024412.01.T01	MTR_6g015910	32.192	146	92	3	6	150	8	147	5.13e-21	93.6
MsG0580024412.01.T01	MTR_6g015910	28.372	215	110	9	113	312	277	462	1.19e-15	77.8
MsG0580024412.01.T01	MTR_7g016040	29.670	182	119	5	1	180	3	177	1.01e-20	92.0
MsG0580024412.01.T01	MTR_7g033040	34.848	132	83	1	3	134	8	136	1.91e-20	92.0
MsG0580024412.01.T01	MTR_7g033040	30.723	166	92	5	143	307	320	463	1.35e-12	68.6
MsG0580024412.01.T01	MTR_7g016040	29.670	182	119	5	1	180	3	177	2.27e-20	91.7
MsG0580024412.01.T01	MTR_7g016040	31.250	144	84	5	170	312	332	461	2.47e-13	70.9
MsG0580024412.01.T01	MTR_8g076510	35.593	118	76	0	32	149	40	157	5.88e-19	87.8
MsG0580024412.01.T01	MTR_8g076510	27.442	215	116	9	111	312	289	476	6.82e-14	72.4
MsG0580024412.01.T01	MTR_8g016180	29.870	154	101	3	2	150	5	156	1.55e-18	86.3
MsG0580024412.01.T01	MTR_8g016180	28.947	152	105	1	2	150	5	156	2.25e-18	85.9
MsG0580024412.01.T01	MTR_8g016180	29.348	184	102	8	111	287	284	446	1.15e-12	68.9
MsG0580024412.01.T01	MTR_5g021220	29.605	152	103	2	2	149	3	154	2.84e-17	82.8
MsG0580024412.01.T01	MTR_5g021220	34.014	147	84	6	165	311	353	486	1.38e-14	74.7
MsG0580024412.01.T01	MTR_5g091760	29.323	133	93	1	20	151	27	159	1.39e-16	80.5
MsG0580024412.01.T01	MTR_4g086130	31.008	129	84	2	30	158	34	157	1.36e-15	77.8
MsG0580024412.01.T01	MTR_3g467140	26.163	172	117	2	10	177	8	173	1.53e-15	77.4
MsG0580024412.01.T01	MTR_8g100135	34.483	116	75	1	31	145	34	149	6.06e-15	75.5
MsG0580024412.01.T01	MTR_8g100135	34.483	116	75	1	31	145	34	149	9.37e-15	75.1
MsG0580024412.01.T01	MTR_3g467130	31.624	117	80	0	31	147	33	149	3.76e-14	73.2
MsG0580024412.01.T01	MTR_8g072260	31.405	121	75	3	28	145	31	146	7.70e-14	72.4
MsG0580024412.01.T01	MTR_8g072260	31.405	121	75	3	28	145	31	146	8.27e-14	72.4
MsG0580024412.01.T01	MTR_8g072260	31.148	122	76	3	27	145	30	146	9.67e-14	72.0
MsG0580024412.01.T01	MTR_1g037580	30.833	120	79	1	28	147	41	156	1.29e-13	71.6
MsG0580024412.01.T01	MTR_4g086040	33.051	118	71	2	30	145	37	148	1.52e-13	71.6
MsG0580024412.01.T01	MTR_5g092150	28.000	125	90	0	23	147	21	145	1.51e-12	68.6
MsG0580024412.01.T01	MTR_5g025610	27.273	132	89	3	31	160	35	161	2.23e-12	67.8
MsG0580024412.01.T01	MTR_5g025610	27.273	132	89	3	31	160	35	161	2.26e-12	67.8
MsG0580024412.01.T01	MTR_5g025610	27.273	132	89	3	31	160	35	161	3.32e-12	67.4
MsG0580024412.01.T01	MTR_5g020020	30.612	147	82	5	170	311	272	403	4.29e-12	67.0
MsG0580024412.01.T01	MTR_3g112120	30.328	122	82	2	23	141	31	152	6.86e-12	66.6
MsG0580024412.01.T01	MTR_1g019410	25.170	147	101	2	6	147	5	147	9.36e-12	66.2
MsG0580024412.01.T01	MTR_6g034940	30.719	153	76	5	162	304	343	475	2.88e-11	64.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024412.01.T01	AT1G12740	70.748	147	43	0	1	147	1	147	2.49e-76	238
MsG0580024412.01.T01	AT1G12740	70.748	147	43	0	1	147	1	147	1.60e-74	237
MsG0580024412.01.T01	AT1G12740	53.425	146	54	4	170	314	338	470	9.65e-40	145
MsG0580024412.01.T01	AT1G12740	70.748	147	43	0	1	147	1	147	1.69e-74	237
MsG0580024412.01.T01	AT1G12740	54.110	146	59	4	170	314	338	476	3.90e-40	147
MsG0580024412.01.T01	AT1G12740	68.627	153	42	1	1	147	1	153	1.95e-72	232
MsG0580024412.01.T01	AT1G12740	53.425	146	54	4	170	314	344	476	1.03e-39	145
MsG0580024412.01.T01	AT1G78490	47.518	141	74	0	3	143	7	147	1.22e-41	149
MsG0580024412.01.T01	AT5G48000	41.420	169	99	0	16	184	20	188	1.78e-41	150
MsG0580024412.01.T01	AT5G48000	32.192	146	87	4	168	313	342	475	1.29e-15	77.8
MsG0580024412.01.T01	AT5G48000	41.420	169	99	0	16	184	20	188	1.78e-41	150
MsG0580024412.01.T01	AT5G48000	32.192	146	87	4	168	313	342	475	1.29e-15	77.8
MsG0580024412.01.T01	AT5G48000	41.420	169	99	0	16	184	20	188	1.78e-41	150
MsG0580024412.01.T01	AT5G48000	32.192	146	87	4	168	313	342	475	1.29e-15	77.8
MsG0580024412.01.T01	AT5G48000	41.420	169	99	0	16	184	20	188	1.78e-41	150
MsG0580024412.01.T01	AT5G48000	32.192	146	87	4	168	313	342	475	1.29e-15	77.8
MsG0580024412.01.T01	AT5G48000	41.420	169	99	0	16	184	20	188	1.78e-41	150
MsG0580024412.01.T01	AT5G48000	32.192	146	87	4	168	313	342	475	1.29e-15	77.8
MsG0580024412.01.T01	AT5G48000	41.420	169	99	0	16	184	20	188	1.78e-41	150
MsG0580024412.01.T01	AT5G48000	32.192	146	87	4	168	313	342	475	1.29e-15	77.8
MsG0580024412.01.T01	AT1G78490	47.518	141	74	0	3	143	7	147	4.09e-41	149
MsG0580024412.01.T01	AT1G78490	31.677	161	95	6	158	317	333	479	2.51e-15	77.0
MsG0580024412.01.T01	AT5G48000	41.420	169	99	0	16	184	61	229	4.14e-41	150
MsG0580024412.01.T01	AT5G48000	32.192	146	87	4	168	313	383	516	1.55e-15	77.8
MsG0580024412.01.T01	AT1G55940	38.144	194	108	6	1	182	13	206	2.10e-38	142
MsG0580024412.01.T01	AT1G55940	36.207	116	62	3	170	285	360	463	4.51e-13	70.1
MsG0580024412.01.T01	AT1G55940	38.144	194	108	6	1	182	13	206	2.94e-38	142
MsG0580024412.01.T01	AT1G55940	35.417	144	81	3	170	313	360	491	5.23e-18	85.1
MsG0580024412.01.T01	AT3G44970	42.857	147	81	1	7	150	8	154	1.84e-35	132
MsG0580024412.01.T01	AT3G44970	42.857	147	81	1	7	150	8	154	1.34e-34	132
MsG0580024412.01.T01	AT3G44970	32.653	147	85	5	168	313	342	475	7.18e-17	81.6
MsG0580024412.01.T01	AT4G15310	37.013	154	90	4	1	150	4	154	1.33e-30	120
MsG0580024412.01.T01	AT4G15300	37.226	137	83	1	15	151	19	152	4.68e-27	111
MsG0580024412.01.T01	AT4G15300	50.746	67	32	1	250	316	407	472	9.00e-13	69.3
MsG0580024412.01.T01	AT4G15300	37.226	137	83	1	15	151	9	142	4.99e-27	110
MsG0580024412.01.T01	AT4G15300	50.746	67	32	1	250	316	397	462	8.92e-13	69.3
MsG0580024412.01.T01	AT1G65670	33.784	148	93	2	6	151	8	152	3.26e-25	105
MsG0580024412.01.T01	AT4G15396	29.609	179	118	4	19	197	23	193	6.95e-24	102
MsG0580024412.01.T01	AT4G15396	29.609	179	118	4	19	197	23	193	8.05e-24	101
MsG0580024412.01.T01	AT4G15393	27.411	197	131	6	2	197	8	193	9.32e-24	100
MsG0580024412.01.T01	AT4G15396	29.609	179	118	4	19	197	23	193	9.56e-24	101
MsG0580024412.01.T01	AT4G15396	28.497	193	127	5	8	197	9	193	1.17e-23	101
MsG0580024412.01.T01	AT4G15393	27.411	197	131	6	2	197	8	193	1.69e-23	100
MsG0580024412.01.T01	AT1G73340	35.374	147	94	1	30	175	42	188	2.28e-22	97.8
MsG0580024412.01.T01	AT1G73340	29.605	152	88	5	170	315	343	481	2.41e-12	67.8
MsG0580024412.01.T01	AT3G50660	34.375	128	84	0	23	150	31	158	3.02e-22	97.1
MsG0580024412.01.T01	AT3G50660	33.784	148	97	1	23	169	31	178	5.89e-22	96.7
MsG0580024412.01.T01	AT3G50660	32.168	143	95	2	170	312	370	510	5.09e-15	76.3
MsG0580024412.01.T01	AT4G15300	34.028	144	84	2	15	150	9	149	7.57e-22	96.3
MsG0580024412.01.T01	AT4G15300	50.746	67	32	1	250	316	419	484	7.44e-13	69.3
MsG0580024412.01.T01	AT5G05690	33.333	120	80	0	31	150	31	150	1.12e-21	95.1
MsG0580024412.01.T01	AT5G05690	33.333	120	80	0	31	150	31	150	2.13e-21	94.7
MsG0580024412.01.T01	AT5G05690	28.249	177	111	5	139	315	309	469	9.30e-12	66.2
MsG0580024412.01.T01	AT4G15393	41.304	92	50	2	2	92	8	96	9.18e-21	92.0
MsG0580024412.01.T01	AT3G30180	32.026	153	97	3	3	154	5	151	9.83e-21	92.8
MsG0580024412.01.T01	AT3G30180	28.641	206	117	7	113	310	278	461	1.94e-13	71.2
MsG0580024412.01.T01	AT3G19270	30.061	163	109	2	1	163	4	161	1.26e-20	92.4
MsG0580024412.01.T01	AT3G19270	30.061	163	109	2	1	163	4	161	1.36e-20	92.4
MsG0580024412.01.T01	AT3G19270	29.348	184	110	6	113	294	279	444	9.58e-12	66.2
MsG0580024412.01.T01	AT5G14400	41.414	99	58	0	36	134	1	99	2.31e-20	90.5
MsG0580024412.01.T01	AT5G14400	41.414	99	58	0	36	134	1	99	9.59e-20	89.4
MsG0580024412.01.T01	AT5G14400	41.414	99	58	0	36	134	1	99	1.58e-19	89.0
MsG0580024412.01.T01	AT4G36380	32.812	128	86	0	31	158	69	196	2.21e-19	89.4
MsG0580024412.01.T01	AT4G36380	27.542	236	120	13	97	316	314	514	8.86e-14	72.4
MsG0580024412.01.T01	AT4G36380	32.812	128	86	0	31	158	69	196	2.46e-19	88.6
MsG0580024412.01.T01	AT5G38970	27.586	203	135	6	3	200	5	200	1.76e-17	83.2
MsG0580024412.01.T01	AT5G38970	26.786	224	147	7	3	221	5	216	2.22e-17	83.2
MsG0580024412.01.T01	AT5G38970	27.273	242	135	9	87	312	247	463	2.03e-14	74.3
MsG0580024412.01.T01	AT4G19230	31.624	117	76	1	31	147	36	148	8.30e-16	78.6
MsG0580024412.01.T01	AT5G45340	26.207	145	103	1	1	145	6	146	1.04e-15	77.8
MsG0580024412.01.T01	AT4G19230	31.624	117	76	1	31	147	36	148	1.16e-15	77.8
MsG0580024412.01.T01	AT5G45340	27.338	139	97	1	7	145	12	146	1.39e-15	77.8
MsG0580024412.01.T01	AT3G13730	31.538	130	81	2	30	151	50	179	5.94e-15	75.9
MsG0580024412.01.T01	AT3G13730	31.538	130	81	2	30	151	50	179	7.14e-15	75.5
MsG0580024412.01.T01	AT3G13730	29.302	215	109	9	111	311	303	488	3.40e-12	67.4
MsG0580024412.01.T01	AT2G29090	29.221	154	101	4	17	170	37	182	1.61e-14	73.9
MsG0580024412.01.T01	AT5G38970	27.572	243	133	10	87	312	166	382	1.62e-14	73.9
MsG0580024412.01.T01	AT5G38970	27.572	243	133	10	87	312	166	382	1.62e-14	73.9
MsG0580024412.01.T01	AT2G29090	29.221	154	101	4	17	170	37	182	2.94e-14	73.6
MsG0580024412.01.T01	AT2G29090	29.221	154	101	4	17	170	37	182	2.94e-14	73.6
MsG0580024412.01.T01	AT5G36110	35.294	119	72	2	31	145	34	151	3.12e-14	73.2
MsG0580024412.01.T01	AT5G36110	35.294	119	72	2	31	145	34	151	1.13e-13	72.0
MsG0580024412.01.T01	AT5G36140	33.621	116	75	2	31	145	33	147	4.00e-13	69.3
MsG0580024412.01.T01	AT1G19630	27.950	161	111	4	3	160	9	167	1.01e-12	68.9
MsG0580024412.01.T01	AT1G19630	25.957	235	142	7	3	234	9	214	1.14e-12	68.9
MsG0580024412.01.T01	AT1G19630	27.950	161	111	4	3	160	9	167	1.69e-12	68.2
MsG0580024412.01.T01	AT5G05690	26.316	209	134	6	111	315	158	350	7.45e-12	65.9

Find 70 sgRNAs with CRISPR-Local

Find 205 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GATACATTCACAGAGATATT+TGG	0.163237	5:-4521686	MsG0580024412.01.T01:CDS
ACCAAACATTTCCTGGCTTT+TGG	0.233382	5:-4519949	MsG0580024412.01.T01:CDS
ACTGAAGGCTCTCACCTAAA+AGG	0.242119	5:+4522130	None:intergenic
TTTGATCTTTCTCTGTGATC+TGG	0.305430	5:+4519661	None:intergenic
AACAATATTCCCTCCTTTGA+TGG	0.311079	5:+4519722	None:intergenic
AATATCTCTGTGAATGTATC+TGG	0.320683	5:+4521689	None:intergenic
AGTGCCTTCCGGAAGATTCC+TGG	0.327774	5:+4520453	None:intergenic
TTCATTCTCTTCTTGATGAA+TGG	0.341050	5:+4522075	None:intergenic
ATACATTCACAGAGATATTT+GGG	0.355848	5:-4521685	MsG0580024412.01.T01:CDS
TGGTGCTACCAAACATTTCC+TGG	0.362057	5:-4519956	MsG0580024412.01.T01:CDS
AACATGATGCTCAATTTGTT+TGG	0.363635	5:-4521614	MsG0580024412.01.T01:CDS
ACCAAGTTTGTCTTGAAGAT+TGG	0.370600	5:+4521797	None:intergenic
ATTTGCAAGTCTCGCAGTTT+CGG	0.376972	5:+4520482	None:intergenic
TTACACGTTGGGTTTATAGA+TGG	0.377880	5:-4522203	MsG0580024412.01.T01:CDS
AATTGAGCATCATGTTCTTA+AGG	0.390570	5:+4521621	None:intergenic
CTGAAGGCTCTCACCTAAAA+GGG	0.394233	5:+4522131	None:intergenic
GGGAATATTGTTCGAACTCC+TGG	0.399698	5:-4519711	MsG0580024412.01.T01:CDS
TAATTTCCCTTAGTGCCTTC+CGG	0.411050	5:+4520442	None:intergenic
CAAAATGTAGGTTCCTTACA+TGG	0.428064	5:-4521659	MsG0580024412.01.T01:CDS
AGACTTGCAAATATAGTTCC+AGG	0.431096	5:-4520471	MsG0580024412.01.T01:CDS
ATATAGTTCCAGGAATCTTC+CGG	0.432579	5:-4520461	MsG0580024412.01.T01:CDS
ATTGCTTGGTAACAAAGTAT+AGG	0.436931	5:-4519864	MsG0580024412.01.T01:intron
ACCAAAAGCCAGGAAATGTT+TGG	0.440923	5:+4519948	None:intergenic
TAACTACTTCATATTCCAAC+AGG	0.445863	5:-4521738	MsG0580024412.01.T01:CDS
TAGGATTCTCACGTCGTTCA+AGG	0.447412	5:+4520657	None:intergenic
CTTATACATAAAACCATGTA+AGG	0.447459	5:+4521646	None:intergenic
ATCTGGACATGAAAGCCATT+TGG	0.457418	5:+4519678	None:intergenic
TATGTGCATTATTACACGTT+GGG	0.458676	5:-4522214	MsG0580024412.01.T01:CDS
GAATCTTCCGGAAGGCACTA+AGG	0.466533	5:-4520449	MsG0580024412.01.T01:CDS
AATATGAAGTAGTTAAGATC+AGG	0.468099	5:+4521746	None:intergenic
CCTGGTTTACAATTTCCAAA+TGG	0.471815	5:-4519693	MsG0580024412.01.T01:CDS
AGGAATATCACAAGAAGTGT+TGG	0.485257	5:+4522098	None:intergenic
AAGAAATGGTCTACAGGGAA+TGG	0.496910	5:-4519986	MsG0580024412.01.T01:CDS
AAACTACCACCAGGTACAAT+GGG	0.497164	5:-4522159	MsG0580024412.01.T01:CDS
ACAATATTCCCTCCTTTGAT+GGG	0.500084	5:+4519723	None:intergenic
GGGTCTGTGGACACTACAAC+TGG	0.501330	5:+4521767	None:intergenic
CCATTTGGAAATTGTAAACC+AGG	0.516872	5:+4519693	None:intergenic
GGAATATCACAAGAAGTGTT+GGG	0.519625	5:+4522099	None:intergenic
TCATGCCTCCACCAAAAGCC+AGG	0.525654	5:+4519938	None:intergenic
ACCAATCTTCAAGACAAACT+TGG	0.526479	5:-4521798	MsG0580024412.01.T01:CDS
AGAATTTGCCAAGGTTCAGA+TGG	0.528615	5:-4519899	MsG0580024412.01.T01:CDS
GGTAGTTTACCATTGCAACT+AGG	0.543466	5:+4522174	None:intergenic
ATTCTCTTCTTGATGAATGG+AGG	0.545192	5:+4522078	None:intergenic
ATCTTCAAGACAAACTTGGT+TGG	0.546380	5:-4521794	MsG0580024412.01.T01:CDS
ATGTATCTGGATACCAACTC+TGG	0.549088	5:+4521702	None:intergenic
ATATTTGGGCAACAAAATGT+AGG	0.549555	5:-4521671	MsG0580024412.01.T01:CDS
AAGGGAAAATATTCCAGAGT+TGG	0.550551	5:-4521715	MsG0580024412.01.T01:CDS
TAAACTACCACCAGGTACAA+TGG	0.556385	5:-4522160	MsG0580024412.01.T01:CDS
ATTCTCACGTCGTTCAAGGA+TGG	0.565772	5:+4520661	None:intergenic
TTCAGGTGGCGACCCATCAA+AGG	0.566577	5:-4519735	MsG0580024412.01.T01:intron
AATCTTCCGGAAGGCACTAA+GGG	0.567870	5:-4520448	MsG0580024412.01.T01:CDS
TGGAGAAATCCTAGTTGCAA+TGG	0.573060	5:-4522183	MsG0580024412.01.T01:CDS
TCATCAAGAAGAGAATGAAA+AGG	0.575267	5:-4522071	MsG0580024412.01.T01:intron
AGGGGTAATCCCATTGTACC+TGG	0.583857	5:+4522150	None:intergenic
GTAGTTAAGATCAGGGTCTG+TGG	0.595135	5:+4521754	None:intergenic
AAATGTTTGGTAGCACCACT+CGG	0.596149	5:+4519961	None:intergenic
CATGTTCTTCCTAAAAGACA+AGG	0.597829	5:+4520687	None:intergenic
AGGTGGCGACCCATCAAAGG+AGG	0.605955	5:-4519732	MsG0580024412.01.T01:CDS
TGTTGGGACAGAATTTGCCA+AGG	0.619174	5:-4519908	MsG0580024412.01.T01:CDS
TACTTCATATTCCAACAGGA+AGG	0.624124	5:-4521734	MsG0580024412.01.T01:CDS
ACTTCATATTCCAACAGGAA+GGG	0.625650	5:-4521733	MsG0580024412.01.T01:CDS
ATATGAAGTAGTTAAGATCA+GGG	0.631648	5:+4521747	None:intergenic
CTATGTGCATTATTACACGT+TGG	0.643076	5:-4522215	MsG0580024412.01.T01:CDS
TGCAATGGTAAACTACCACC+AGG	0.650281	5:-4522168	MsG0580024412.01.T01:CDS
TCCTAAAAGACAAGGTACCG+AGG	0.652979	5:+4520695	None:intergenic
TGAAGGCTCTCACCTAAAAG+GGG	0.655464	5:+4522132	None:intergenic
GGTGGCGACCCATCAAAGGA+GGG	0.671094	5:-4519731	MsG0580024412.01.T01:CDS
AGTTCCAGGAATCTTCCGGA+AGG	0.672126	5:-4520457	MsG0580024412.01.T01:CDS
GGTAATCCCATTGTACCTGG+TGG	0.706713	5:+4522153	None:intergenic
CTACAGGGAATGGAACCGAG+TGG	0.755309	5:-4519976	MsG0580024412.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	GTTACAAATATATTATTATA+TGG	-	Chr5:4521102-4521121	MsG0580024412.01.T01:intron	10.0%
!!	AAAAATAGTTCAAAAGATTA+AGG	+	Chr5:4521128-4521147	None:intergenic	15.0%
!!	AATTATAAAAATTAGTACAC+TGG	-	Chr5:4520695-4520714	MsG0580024412.01.T01:CDS	15.0%
!!!	AACTATTTTTTTTATGCTTT+AGG	-	Chr5:4521138-4521157	MsG0580024412.01.T01:intron	15.0%
!!!	CTTCATTTTTATTTTTATGT+GGG	-	Chr5:4521304-4521323	MsG0580024412.01.T01:intron	15.0%
!!!	TCTTCATTTTTATTTTTATG+TGG	-	Chr5:4521303-4521322	MsG0580024412.01.T01:intron	15.0%
!!	ACAAAAACAAAGGAATAATA+TGG	-	Chr5:4520023-4520042	MsG0580024412.01.T01:CDS	20.0%
!!	ATCACAAAATTGTAAAAAGA+AGG	+	Chr5:4521861-4521880	None:intergenic	20.0%
!!	TCACAAAATTGTAAAAAGAA+GGG	+	Chr5:4521860-4521879	None:intergenic	20.0%
!!	TCATCTGAATGATTATATAT+AGG	+	Chr5:4520484-4520503	None:intergenic	20.0%
!!	TGATTATATATAGGAAAGAA+AGG	+	Chr5:4520475-4520494	None:intergenic	20.0%
!!!	ACTAAGGGAAATTAATTTTA+AGG	-	Chr5:4521439-4521458	MsG0580024412.01.T01:intron	20.0%
!!!	AGGTAAGCATTTTATTAATA+AGG	-	Chr5:4520647-4520666	MsG0580024412.01.T01:intron	20.0%
!!!	CTAAGGGAAATTAATTTTAA+GGG	-	Chr5:4521440-4521459	MsG0580024412.01.T01:intron	20.0%
!!!	GGTAAGCATTTTATTAATAA+GGG	-	Chr5:4520648-4520667	MsG0580024412.01.T01:intron	20.0%
!!!	TAATAATATCACAGTTTGTT+CGG	-	Chr5:4519889-4519908	MsG0580024412.01.T01:CDS	20.0%
!	AACTTTGTTGCATTTATACA+AGG	-	Chr5:4520571-4520590	MsG0580024412.01.T01:intron	25.0%
!	AATATGAAGTAGTTAAGATC+AGG	+	Chr5:4520129-4520148	None:intergenic	25.0%
!	ACTTGATGTTTAAGTAATAG+TGG	-	Chr5:4521524-4521543	MsG0580024412.01.T01:intron	25.0%
!	ATAAAAATTAGTACACTGGT+TGG	-	Chr5:4520699-4520718	MsG0580024412.01.T01:CDS	25.0%
!	ATACATTCACAGAGATATTT+GGG	-	Chr5:4520187-4520206	MsG0580024412.01.T01:intron	25.0%
!	ATATGAAGTAGTTAAGATCA+GGG	+	Chr5:4520128-4520147	None:intergenic	25.0%
!	ATCAATGACATTTACATTTC+AGG	-	Chr5:4521266-4521285	MsG0580024412.01.T01:intron	25.0%
!	ATTGAAGAACTTAGAAAAGA+GGG	-	Chr5:4520939-4520958	MsG0580024412.01.T01:intron	25.0%
!	CTTATACATAAAACCATGTA+AGG	+	Chr5:4520229-4520248	None:intergenic	25.0%
!	CTTTAACGTTTGAATATTTC+AGG	-	Chr5:4522120-4522139	MsG0580024412.01.T01:CDS	25.0%
!	GATAACTTGAGTTCAAATTA+TGG	-	Chr5:4519912-4519931	MsG0580024412.01.T01:CDS	25.0%
!	TATTGAAGAACTTAGAAAAG+AGG	-	Chr5:4520938-4520957	MsG0580024412.01.T01:intron	25.0%
!	TCATTATTGTAGACAAAATG+AGG	+	Chr5:4521577-4521596	None:intergenic	25.0%
!!	AATAATATCACAGTTTGTTC+GGG	-	Chr5:4519890-4519909	MsG0580024412.01.T01:CDS	25.0%
!!	TAATTTGATGGCATAAGTAA+GGG	+	Chr5:4521042-4521061	None:intergenic	25.0%
!!	TTTTGTGATCCAAAAAGAAA+TGG	-	Chr5:4521872-4521891	MsG0580024412.01.T01:intron	25.0%
!!!	ACAGCAAAATTAACTTGTTT+TGG	-	Chr5:4520816-4520835	MsG0580024412.01.T01:intron	25.0%
!!!	ATCATCTTTTTAAGACTTTC+AGG	+	Chr5:4520285-4520304	None:intergenic	25.0%
	AAAGGTTTAGACTTTAGACA+AGG	+	Chr5:4520457-4520476	None:intergenic	30.0%
	AAAGTTCTCCCTTAAATTCT+CGG	+	Chr5:4520557-4520576	None:intergenic	30.0%
	AATATCTCTGTGAATGTATC+TGG	+	Chr5:4520186-4520205	None:intergenic	30.0%
	AATTGAGCATCATGTTCTTA+AGG	+	Chr5:4520254-4520273	None:intergenic	30.0%
	ACGCATTCTGAATTAAATTC+AGG	+	Chr5:4522095-4522114	None:intergenic	30.0%
	AGCTTATAACAAATGTTTGC+AGG	-	Chr5:4520627-4520646	MsG0580024412.01.T01:intron	30.0%
	ATATTTGGGCAACAAAATGT+AGG	-	Chr5:4520201-4520220	MsG0580024412.01.T01:intron	30.0%
	ATTGTCAGAGAGTAATTTGA+TGG	+	Chr5:4521054-4521073	None:intergenic	30.0%
	GAAAAGATGGAAAGAAAATG+AGG	+	Chr5:4521735-4521754	None:intergenic	30.0%
	GAAGAAGGTTAAAATAGTAG+AGG	+	Chr5:4520430-4520449	None:intergenic	30.0%
	GATACATTCACAGAGATATT+TGG	-	Chr5:4520186-4520205	MsG0580024412.01.T01:intron	30.0%
	GTAATAAGCAATGTAATGCA+TGG	-	Chr5:4522030-4522049	MsG0580024412.01.T01:intron	30.0%
	TAACGTTTGAATATTTCAGG+TGG	-	Chr5:4522123-4522142	MsG0580024412.01.T01:CDS	30.0%
	TAACTACTTCATATTCCAAC+AGG	-	Chr5:4520134-4520153	MsG0580024412.01.T01:intron	30.0%
	TATGTGCATTATTACACGTT+GGG	-	Chr5:4519658-4519677	MsG0580024412.01.T01:CDS	30.0%
	TCATCAAGAAGAGAATGAAA+AGG	-	Chr5:4519801-4519820	MsG0580024412.01.T01:intron	30.0%
	TCTTAAAAAGATGATCTCAG+AGG	-	Chr5:4520290-4520309	MsG0580024412.01.T01:intron	30.0%
	TTAAATTCAGGCGTTAAAGA+AGG	+	Chr5:4522083-4522102	None:intergenic	30.0%
	TTCATTCTCTTCTTGATGAA+TGG	+	Chr5:4519800-4519819	None:intergenic	30.0%
	TTTAGACTTTAGACAAGGAA+TGG	+	Chr5:4520452-4520471	None:intergenic	30.0%
!	AACATGATGCTCAATTTGTT+TGG	-	Chr5:4520258-4520277	MsG0580024412.01.T01:intron	30.0%
!	ATTGCTTGGTAACAAAGTAT+AGG	-	Chr5:4522008-4522027	MsG0580024412.01.T01:intron	30.0%
!	GTAATTTGATGGCATAAGTA+AGG	+	Chr5:4521043-4521062	None:intergenic	30.0%
!	TTGTTGTCTCTTTTCAGATA+TGG	-	Chr5:4520051-4520070	MsG0580024412.01.T01:CDS	30.0%
	AACAATATTCCCTCCTTTGA+TGG	+	Chr5:4522153-4522172	None:intergenic	35.0%
	AAGGGAAAATATTCCAGAGT+TGG	-	Chr5:4520157-4520176	MsG0580024412.01.T01:intron	35.0%
	ACAATATTCCCTCCTTTGAT+GGG	+	Chr5:4522152-4522171	None:intergenic	35.0%
	ACCAAGTTTGTCTTGAAGAT+TGG	+	Chr5:4520078-4520097	None:intergenic	35.0%
	ACCAATCTTCAAGACAAACT+TGG	-	Chr5:4520074-4520093	MsG0580024412.01.T01:CDS	35.0%
	ACTTCATATTCCAACAGGAA+GGG	-	Chr5:4520139-4520158	MsG0580024412.01.T01:intron	35.0%
	AGACTTGCAAATATAGTTCC+AGG	-	Chr5:4521401-4521420	MsG0580024412.01.T01:intron	35.0%
	ATATAGTTCCAGGAATCTTC+CGG	-	Chr5:4521411-4521430	MsG0580024412.01.T01:intron	35.0%
	ATCTTCAAGACAAACTTGGT+TGG	-	Chr5:4520078-4520097	MsG0580024412.01.T01:CDS	35.0%
	ATTCTCTTCTTGATGAATGG+AGG	+	Chr5:4519797-4519816	None:intergenic	35.0%
	CAAAATGTAGGTTCCTTACA+TGG	-	Chr5:4520213-4520232	MsG0580024412.01.T01:intron	35.0%
	CATGTTCTTCCTAAAAGACA+AGG	+	Chr5:4521188-4521207	None:intergenic	35.0%
	CCAAAAAGAAATGGTCTACA+GGG	-	Chr5:4521881-4521900	MsG0580024412.01.T01:intron	35.0%
	CCATTTGGAAATTGTAAACC+AGG	+	Chr5:4522182-4522201	None:intergenic	35.0%
	CCTGGTTTACAATTTCCAAA+TGG	-	Chr5:4522179-4522198	MsG0580024412.01.T01:CDS	35.0%
	CTATGTGCATTATTACACGT+TGG	-	Chr5:4519657-4519676	MsG0580024412.01.T01:CDS	35.0%
	GTTTCAATGTTCAAGTGCAT+TGG	-	Chr5:4521499-4521518	MsG0580024412.01.T01:intron	35.0%
	TACTTCATATTCCAACAGGA+AGG	-	Chr5:4520138-4520157	MsG0580024412.01.T01:intron	35.0%
	TCCAAAAAGAAATGGTCTAC+AGG	-	Chr5:4521880-4521899	MsG0580024412.01.T01:intron	35.0%
	TGTAATTGCTTGAACACCAA+GGG	+	Chr5:4521077-4521096	None:intergenic	35.0%
	TGTTGGGAGAAAAAAACTGA+AGG	+	Chr5:4519760-4519779	None:intergenic	35.0%
	TTACACGTTGGGTTTATAGA+TGG	-	Chr5:4519669-4519688	MsG0580024412.01.T01:CDS	35.0%
	TTGTAATTGCTTGAACACCA+AGG	+	Chr5:4521078-4521097	None:intergenic	35.0%
	TTTAGACAAGGAATGGAAGA+AGG	+	Chr5:4520445-4520464	None:intergenic	35.0%
	TTTGATCTTTCTCTGTGATC+TGG	+	Chr5:4522214-4522233	None:intergenic	35.0%
!	AGGAATATCACAAGAAGTGT+TGG	+	Chr5:4519777-4519796	None:intergenic	35.0%
!!	AGCAACTAGCTTTGAAAAGA+TGG	+	Chr5:4521748-4521767	None:intergenic	35.0%
!!	CATTTGTTATAAGCTGTTCC+TGG	+	Chr5:4520622-4520641	None:intergenic	35.0%
!!	GGAATATCACAAGAAGTGTT+GGG	+	Chr5:4519776-4519795	None:intergenic	35.0%
!!!	GATGTCAATTTTGACTTCCT+CGG	-	Chr5:4521160-4521179	MsG0580024412.01.T01:intron	35.0%
!!!	TAACTTGTTTTGGCTTCTCA+GGG	-	Chr5:4520826-4520845	MsG0580024412.01.T01:intron	35.0%
!!!	TTAACTTGTTTTGGCTTCTC+AGG	-	Chr5:4520825-4520844	MsG0580024412.01.T01:intron	35.0%
	AAAAACTGCCATCTGAACCT+TGG	+	Chr5:4521984-4522003	None:intergenic	40.0%
	AAACTACCACCAGGTACAAT+GGG	-	Chr5:4519713-4519732	MsG0580024412.01.T01:CDS	40.0%
	AAATGCCTAGGAAACACAGA+AGG	-	Chr5:4520899-4520918	MsG0580024412.01.T01:intron	40.0%
	AAGAAATGGTCTACAGGGAA+TGG	-	Chr5:4521886-4521905	MsG0580024412.01.T01:intron	40.0%
	ACCAAAAGCCAGGAAATGTT+TGG	+	Chr5:4521927-4521946	None:intergenic	40.0%
	AGAATTTGCCAAGGTTCAGA+TGG	-	Chr5:4521973-4521992	MsG0580024412.01.T01:intron	40.0%
	ATATACCATACCAGCAGGAT+GGG	-	Chr5:4521608-4521627	MsG0580024412.01.T01:CDS	40.0%
	ATCTGGACATGAAAGCCATT+TGG	+	Chr5:4522197-4522216	None:intergenic	40.0%
	ATGTATCTGGATACCAACTC+TGG	+	Chr5:4520173-4520192	None:intergenic	40.0%
	ATTACTCTCTGACAATCCCT+TGG	-	Chr5:4521058-4521077	MsG0580024412.01.T01:intron	40.0%
	ATTATGGCTGAAGCAATCCT+TGG	-	Chr5:4519928-4519947	MsG0580024412.01.T01:CDS	40.0%
	ATTTGCAAGTCTCGCAGTTT+CGG	+	Chr5:4521393-4521412	None:intergenic	40.0%
	CTACAATAATGAACACGTGC+AGG	-	Chr5:4521585-4521604	MsG0580024412.01.T01:CDS	40.0%
	CTTAACAGAAGCAATAGCTC+TGG	-	Chr5:4520968-4520987	MsG0580024412.01.T01:intron	40.0%
	GAAATCCTTCTGTGTTTCCT+AGG	+	Chr5:4520907-4520926	None:intergenic	40.0%
	GCCAGACTTTACTTACCTTT+CGG	-	Chr5:4519950-4519969	MsG0580024412.01.T01:CDS	40.0%
	GGTAGTTTACCATTGCAACT+AGG	+	Chr5:4519701-4519720	None:intergenic	40.0%
	TAAACTACCACCAGGTACAA+TGG	-	Chr5:4519712-4519731	MsG0580024412.01.T01:CDS	40.0%
	TAATTTCCCTTAGTGCCTTC+CGG	+	Chr5:4521433-4521452	None:intergenic	40.0%
	TGGAGAAATCCTAGTTGCAA+TGG	-	Chr5:4519689-4519708	MsG0580024412.01.T01:CDS	40.0%
	TTTGAACCCTGCGAAATATC+AGG	-	Chr5:4521657-4521676	MsG0580024412.01.T01:CDS	40.0%
!	AAATGTTTGGTAGCACCACT+CGG	+	Chr5:4521914-4521933	None:intergenic	40.0%
!	ACAATGGGATTACCCCTTTT+AGG	-	Chr5:4519728-4519747	MsG0580024412.01.T01:CDS	40.0%
!	CAAAGAGAAGCACAAACATG+AGG	+	Chr5:4520995-4521014	None:intergenic	40.0%
!	CCCTGTAGACCATTTCTTTT+TGG	+	Chr5:4521884-4521903	None:intergenic	40.0%
!	GAGGCATGAGATTTTGTGTT+GGG	-	Chr5:4521948-4521967	MsG0580024412.01.T01:intron	40.0%
!	GGAATATTTTCCCTTCCTGT+TGG	+	Chr5:4520152-4520171	None:intergenic	40.0%
!	GGCAGTTTTTCTTCATTGCT+TGG	-	Chr5:4521994-4522013	MsG0580024412.01.T01:intron	40.0%
!	GTGGGCTAAGACAAAAACAA+AGG	-	Chr5:4520013-4520032	MsG0580024412.01.T01:CDS	40.0%
!!	AAACATTTCCTGGCTTTTGG+TGG	-	Chr5:4521926-4521945	MsG0580024412.01.T01:intron	40.0%
!!	ACCAAACATTTCCTGGCTTT+TGG	-	Chr5:4521923-4521942	MsG0580024412.01.T01:intron	40.0%
!!!	GTTGGTTTTCTCAAGAAGTG+TGG	-	Chr5:4520717-4520736	MsG0580024412.01.T01:intron	40.0%
!!!	TTGTTTTTGTCTTAGCCCAC+CGG	+	Chr5:4520013-4520032	None:intergenic	40.0%
	AAGCTTCGAAACCACTTCTC+AGG	-	Chr5:4521016-4521035	MsG0580024412.01.T01:intron	45.0%
	ACTGAAGGCTCTCACCTAAA+AGG	+	Chr5:4519745-4519764	None:intergenic	45.0%
	AGAGGATCCTGATATTTCGC+AGG	+	Chr5:4521667-4521686	None:intergenic	45.0%
	AGCCTGCAGAACATTACAAC+AGG	-	Chr5:4520323-4520342	MsG0580024412.01.T01:intron	45.0%
	AGGAAAGAAGGGAAATGCCT+AGG	-	Chr5:4520887-4520906	MsG0580024412.01.T01:intron	45.0%
	AGGGTTCAAATGTACAGCTG+GGG	+	Chr5:4521647-4521666	None:intergenic	45.0%
	AGGTAAGTAAAGTCTGGCCA+AGG	+	Chr5:4519948-4519967	None:intergenic	45.0%
	ATAAGTAAGGGCCTGAGAAG+TGG	+	Chr5:4521030-4521049	None:intergenic	45.0%
	ATTCTCACGTCGTTCAAGGA+TGG	+	Chr5:4521214-4521233	None:intergenic	45.0%
	CAGGGTTCAAATGTACAGCT+GGG	+	Chr5:4521648-4521667	None:intergenic	45.0%
	CCTTAAATTCTCGGAGGACT+CGG	+	Chr5:4520548-4520567	None:intergenic	45.0%
	CGAGTCCTCCGAGAATTTAA+GGG	-	Chr5:4520546-4520565	MsG0580024412.01.T01:intron	45.0%
	CTGAAGGCTCTCACCTAAAA+GGG	+	Chr5:4519744-4519763	None:intergenic	45.0%
	CTGTTCCTGGAATGTTGAGA+GGG	+	Chr5:4520609-4520628	None:intergenic	45.0%
	GAGGATCCTGATATTTCGCA+GGG	+	Chr5:4521666-4521685	None:intergenic	45.0%
	GATATACCATACCAGCAGGA+TGG	-	Chr5:4521607-4521626	MsG0580024412.01.T01:CDS	45.0%
	GCCGAAAGGTAAGTAAAGTC+TGG	+	Chr5:4519954-4519973	None:intergenic	45.0%
	GCTGAAGGAAGCAACAGAAA+CGG	-	Chr5:4520367-4520386	MsG0580024412.01.T01:intron	45.0%
	GGGAATATTGTTCGAACTCC+TGG	-	Chr5:4522161-4522180	MsG0580024412.01.T01:CDS	45.0%
	GTAGTTAAGATCAGGGTCTG+TGG	+	Chr5:4520121-4520140	None:intergenic	45.0%
	GTTCTCCCTTAAATTCTCGG+AGG	+	Chr5:4520554-4520573	None:intergenic	45.0%
	TAATCCAAAGTTCGGCCGAA+AGG	+	Chr5:4519968-4519987	None:intergenic	45.0%
	TAGGATTCTCACGTCGTTCA+AGG	+	Chr5:4521218-4521237	None:intergenic	45.0%
	TCATTCCCTCTCAACATTCC+AGG	-	Chr5:4520601-4520620	MsG0580024412.01.T01:intron	45.0%
	TGAAGGCTCTCACCTAAAAG+GGG	+	Chr5:4519743-4519762	None:intergenic	45.0%
	TGCAATGGTAAACTACCACC+AGG	-	Chr5:4519704-4519723	MsG0580024412.01.T01:CDS	45.0%
	TGTCTTCAACCCATCCAGAT+GGG	-	Chr5:4521687-4521706	MsG0580024412.01.T01:CDS	45.0%
	TGTTGGGACAGAATTTGCCA+AGG	-	Chr5:4521964-4521983	MsG0580024412.01.T01:intron	45.0%
	TTGTCTTCAACCCATCCAGA+TGG	-	Chr5:4521686-4521705	MsG0580024412.01.T01:CDS	45.0%
!	TCCTCGGTACCTTGTCTTTT+AGG	-	Chr5:4521176-4521195	MsG0580024412.01.T01:intron	45.0%
!!	AATCTTCCGGAAGGCACTAA+GGG	-	Chr5:4521424-4521443	MsG0580024412.01.T01:intron	45.0%
!!	ATCCTAACTCTGGAGTCACA+TGG	-	Chr5:4521232-4521251	MsG0580024412.01.T01:intron	45.0%
!!	GGAGGCATGAGATTTTGTGT+TGG	-	Chr5:4521947-4521966	MsG0580024412.01.T01:intron	45.0%
!!	GGTTTTGTGTCTAGGAGCAT+TGG	-	Chr5:4519631-4519650	MsG0580024412.01.T01:CDS	45.0%
!!	TCCTAAAAGACAAGGTACCG+AGG	+	Chr5:4521180-4521199	None:intergenic	45.0%
!!	TGGTGCTACCAAACATTTCC+TGG	-	Chr5:4521916-4521935	MsG0580024412.01.T01:intron	45.0%
	AGAGCATGCTGCAGGAAAGA+AGG	-	Chr5:4520875-4520894	MsG0580024412.01.T01:intron	50.0%
	AGGGGTAATCCCATTGTACC+TGG	+	Chr5:4519725-4519744	None:intergenic	50.0%
	AGTTCCAGGAATCTTCCGGA+AGG	-	Chr5:4521415-4521434	MsG0580024412.01.T01:intron	50.0%
	CCGAGTCCTCCGAGAATTTA+AGG	-	Chr5:4520545-4520564	MsG0580024412.01.T01:intron	50.0%
	CGACGTGAGAATCCTAACTC+TGG	-	Chr5:4521222-4521241	MsG0580024412.01.T01:intron	50.0%
	CGCCTGTTGTAATGTTCTGC+AGG	+	Chr5:4520328-4520347	None:intergenic	50.0%
	CTTACCTTTCGGCCGAACTT+TGG	-	Chr5:4519961-4519980	MsG0580024412.01.T01:CDS	50.0%
	GAGCATGCTGCAGGAAAGAA+GGG	-	Chr5:4520876-4520895	MsG0580024412.01.T01:intron	50.0%
	GCAGGATATACCATACCAGC+AGG	-	Chr5:4521603-4521622	MsG0580024412.01.T01:CDS	50.0%
	GCAGGGTTCAAATGTACAGC+TGG	+	Chr5:4521649-4521668	None:intergenic	50.0%
	GCTGTTCCTGGAATGTTGAG+AGG	+	Chr5:4520610-4520629	None:intergenic	50.0%
	GGCCCTGGTAATCCAAAGTT+CGG	+	Chr5:4519976-4519995	None:intergenic	50.0%
	GGCCGAACTTTGGATTACCA+GGG	-	Chr5:4519971-4519990	MsG0580024412.01.T01:CDS	50.0%
	GGGTTCAAATGTACAGCTGG+GGG	+	Chr5:4521646-4521665	None:intergenic	50.0%
	GGTAATCCCATTGTACCTGG+TGG	+	Chr5:4519722-4519741	None:intergenic	50.0%
	TGCCATGTGACTCCAGAGTT+AGG	+	Chr5:4521237-4521256	None:intergenic	50.0%
!	AAATTCTCGGAGGACTCGGT+TGG	+	Chr5:4520544-4520563	None:intergenic	50.0%
!	ATTACAACAGGCGTCGTGTC+AGG	-	Chr5:4520335-4520354	MsG0580024412.01.T01:intron	50.0%
!	GATGCTTAAGAGCATGCTGC+AGG	-	Chr5:4520867-4520886	MsG0580024412.01.T01:intron	50.0%
!!	CATTTCCTGGCTTTTGGTGG+AGG	-	Chr5:4521929-4521948	MsG0580024412.01.T01:intron	50.0%
!!	CTGGATGGGTTGAAGACAAG+AGG	+	Chr5:4521685-4521704	None:intergenic	50.0%
!!	GAATCTTCCGGAAGGCACTA+AGG	-	Chr5:4521423-4521442	MsG0580024412.01.T01:intron	50.0%
	AAACGCACCTCCCATCTGGA+TGG	+	Chr5:4521700-4521719	None:intergenic	55.0%
	AACGCACCTCCCATCTGGAT+GGG	+	Chr5:4521699-4521718	None:intergenic	55.0%
	ACCAGCAGGATGGGCAATCA+TGG	-	Chr5:4521617-4521636	MsG0580024412.01.T01:CDS	55.0%
	ACCATGATTGCCCATCCTGC+TGG	+	Chr5:4521621-4521640	None:intergenic	55.0%
	AGTGCCTTCCGGAAGATTCC+TGG	+	Chr5:4521422-4521441	None:intergenic	55.0%
	CGGCCGAACTTTGGATTACC+AGG	-	Chr5:4519970-4519989	MsG0580024412.01.T01:CDS	55.0%
	CTACAGGGAATGGAACCGAG+TGG	-	Chr5:4521896-4521915	MsG0580024412.01.T01:intron	55.0%
	CTCATTCTCCTGGAACCGGT+GGG	-	Chr5:4519995-4520014	MsG0580024412.01.T01:CDS	55.0%
	CTTCAACCCATCCAGATGGG+AGG	-	Chr5:4521690-4521709	MsG0580024412.01.T01:CDS	55.0%
	GATTGCCCATCCTGCTGGTA+TGG	+	Chr5:4521616-4521635	None:intergenic	55.0%
	GGGTCTGTGGACACTACAAC+TGG	+	Chr5:4520108-4520127	None:intergenic	55.0%
	TCATGCCTCCACCAAAAGCC+AGG	+	Chr5:4521937-4521956	None:intergenic	55.0%
	TGCTAAACGCACCTCCCATC+TGG	+	Chr5:4521704-4521723	None:intergenic	55.0%
	TTACCAGGGCCTCATTCTCC+TGG	-	Chr5:4519985-4520004	MsG0580024412.01.T01:CDS	55.0%
	TTCAGGTGGCGACCCATCAA+AGG	-	Chr5:4522137-4522156	MsG0580024412.01.T01:CDS	55.0%
	AGGTGGCGACCCATCAAAGG+AGG	-	Chr5:4522140-4522159	MsG0580024412.01.T01:CDS	60.0%
	CCTCATTCTCCTGGAACCGG+TGG	-	Chr5:4519994-4520013	MsG0580024412.01.T01:CDS	60.0%
	GGGCCTCATTCTCCTGGAAC+CGG	-	Chr5:4519991-4520010	MsG0580024412.01.T01:CDS	60.0%
	GGTGGCGACCCATCAAAGGA+GGG	-	Chr5:4522141-4522160	MsG0580024412.01.T01:CDS	60.0%
	GTCAGGACAGGTCGAGCTGA+AGG	-	Chr5:4520352-4520371	MsG0580024412.01.T01:intron	60.0%
	GTTCCAGGAGAATGAGGCCC+TGG	+	Chr5:4519991-4520010	None:intergenic	60.0%
!	AACAGGCGTCGTGTCAGGAC+AGG	-	Chr5:4520340-4520359	MsG0580024412.01.T01:intron	60.0%
!!	CCACCGGTTCCAGGAGAATG+AGG	+	Chr5:4519997-4520016	None:intergenic	60.0%
!!	TGTCTTAGCCCACCGGTTCC+AGG	+	Chr5:4520006-4520025	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	4519626	4522268	4519626	ID=MsG0580024412.01;Name=MsG0580024412.01
Chr5	mRNA	4519626	4522268	4519626	ID=MsG0580024412.01.T01;Parent=MsG0580024412.01;Name=MsG0580024412.01.T01;_AED=0.32;_eAED=0.45;_QI=0\|0\|0\|1\|1\|1\|6\|0\|327
Chr5	exon	4522072	4522268	4522072	ID=MsG0580024412.01.T01:exon:1414;Parent=MsG0580024412.01.T01
Chr5	exon	4521583	4521826	4521583	ID=MsG0580024412.01.T01:exon:1413;Parent=MsG0580024412.01.T01
Chr5	exon	4520669	4520734	4520669	ID=MsG0580024412.01.T01:exon:1412;Parent=MsG0580024412.01.T01
Chr5	exon	4520433	4520511	4520433	ID=MsG0580024412.01.T01:exon:1411;Parent=MsG0580024412.01.T01
Chr5	exon	4519865	4520135	4519865	ID=MsG0580024412.01.T01:exon:1410;Parent=MsG0580024412.01.T01
Chr5	exon	4519626	4519752	4519626	ID=MsG0580024412.01.T01:exon:1409;Parent=MsG0580024412.01.T01
Chr5	CDS	4522072	4522268	4522072	ID=MsG0580024412.01.T01:cds;Parent=MsG0580024412.01.T01
Chr5	CDS	4521583	4521826	4521583	ID=MsG0580024412.01.T01:cds;Parent=MsG0580024412.01.T01
Chr5	CDS	4520669	4520734	4520669	ID=MsG0580024412.01.T01:cds;Parent=MsG0580024412.01.T01
Chr5	CDS	4520433	4520511	4520433	ID=MsG0580024412.01.T01:cds;Parent=MsG0580024412.01.T01
Chr5	CDS	4519865	4520135	4519865	ID=MsG0580024412.01.T01:cds;Parent=MsG0580024412.01.T01
Chr5	CDS	4519626	4519752	4519626	ID=MsG0580024412.01.T01:cds;Parent=MsG0580024412.01.T01

Gene Sequence

>MsG0580024412.01.T01

ATGTGGGTTTTGTGTCTAGGAGCATTGGTTACTATGTGCATTATTACACGTTGGGTTTATAGATGGAGAAATCCTAGTTGCAATGGTAAACTACCACCAGGTACAATGGGATTACCCCTTTTAGGTGAGAGCCTTCAGTTTTTTTCTCCCAACACTTCTTGTGATATTCCTCCATTCATCAAGAAGAGAATGAAAAGATATGGACCAATCTTCAAGACAAACTTGGTTGGAAGACCAGTTGTAGTGTCCACAGACCCTGATCTTAACTACTTCATATTCCAACAGGAAGGGAAAATATTCCAGAGTTGGTATCCAGATACATTCACAGAGATATTTGGGCAACAAAATGTAGGTTCCTTACATGGTTTTATGTATAAGTACCTTAAGAACATGATGCTCAATTTGTTTGGTCCTGAAAGTCTTAAAAAGATGATCTCAGAGGATGTCAATTTTGACTTCCTCGGTACCTTGTCTTTTAGGAAGAACATGAAGCCATCCTTGAACGACCTCATAACCGAAACTGCGAGACTTGCAAATATAGTTCCAGGAATCTTCCGGAAGGCACTAAGGGAAATTAATTTTAAGGCTAGTTGCTTCAAAGAATCTCAAAAAGTAATGCAATTAGCTTCTTTAGCAAGTACTATAGATAATCATTCAAGTAACTTAGATTCTAGTCTATCATGTCCCTTCTTTTTACAATTTTGTGATCCAAAAAGAAATGGTCTACAGGGAATGGAACCGAGTGGTGCTACCAAACATTTCCTGGCTTTTGGTGGAGGCATGAGATTTTGTGTTGGGACAGAATTTGCCAAGGTTCAGATGGCAGTTTTTCTTCATTGCTTGGTAACAAAGTATAGGTGGCGACCCATCAAAGGAGGGAATATTGTTCGAACTCCTGGTTTACAATTTCCAAATGGCTTTCATGTCCAGATCACAGAGAAAGATCAAAAGAAGCACGAATCAGAATGTACAACTACATATTAG

Protein sequence

>MsG0580024412.01.T01

MWVLCLGALVTMCIITRWVYRWRNPSCNGKLPPGTMGLPLLGESLQFFSPNTSCDIPPFIKKRMKRYGPIFKTNLVGRPVVVSTDPDLNYFIFQQEGKIFQSWYPDTFTEIFGQQNVGSLHGFMYKYLKNMMLNLFGPESLKKMISEDVNFDFLGTLSFRKNMKPSLNDLITETARLANIVPGIFRKALREINFKASCFKESQKVMQLASLASTIDNHSSNLDSSLSCPFFLQFCDPKRNGLQGMEPSGATKHFLAFGGGMRFCVGTEFAKVQMAVFLHCLVTKYRWRPIKGGNIVRTPGLQFPNGFHVQITEKDQKKHESECTTTY*