AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580024698.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580024698.01.T01 MTR_5g014710 98.969 97 1 0 12 108 262 358 2.12e-63 196
MsG0580024698.01.T01 MTR_4g092690 76.289 97 23 0 12 108 260 356 2.89e-48 157
MsG0580024698.01.T01 MTR_8g463760 61.616 99 37 1 8 105 242 340 1.32e-36 127
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580024698.01.T01 AT2G22780 73.196 97 26 0 12 108 258 354 1.19e-45 150
MsG0580024698.01.T01 AT1G53240 69.149 94 29 0 12 105 245 338 8.83e-41 137
MsG0580024698.01.T01 AT3G15020 64.894 94 33 0 12 105 245 338 6.12e-39 133
MsG0580024698.01.T01 AT5G09660 72.165 97 27 0 12 108 237 333 1.22e-36 127
MsG0580024698.01.T01 AT5G09660 72.165 97 27 0 12 108 257 353 1.89e-36 127
MsG0580024698.01.T01 AT5G09660 72.165 97 27 0 12 108 258 354 2.05e-36 126
MsG0580024698.01.T01 AT3G47520 54.255 94 41 1 12 103 298 391 1.97e-28 106
MsG0580024698.01.T01 AT3G15020 64.912 57 20 0 12 68 245 301 1.81e-20 84.0
MsG0580024698.01.T01 AT3G53910 32.558 86 56 1 25 108 9 94 6.88e-14 62.8

Find 32 sgRNAs with CRISPR-Local

Find 83 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTTGTTGCTAACTCTTTCT+TGG 0.221091 5:+8385365 None:intergenic
ATATTTCAACAGGGCAAGTT+TGG 0.323504 5:-8385394 MsG0580024698.01.T01:intron
TGCAGATCCAGCTCCAGCTT+TGG 0.369553 5:+8386135 None:intergenic
AATACAGGTTCGCTATTTGA+TGG 0.372876 5:+8385210 None:intergenic
GTGAGAGACAGCAGAGAGGC+TGG 0.394122 5:-8386510 MsG0580024698.01.T01:CDS
CTCTAGGCCAAAGCTGGAGC+TGG 0.397270 5:-8386142 MsG0580024698.01.T01:intron
GTTGAGGAATTTCTTCCTCT+TGG 0.402166 5:-8385525 MsG0580024698.01.T01:CDS
TTATTACACTTATCTAATAC+AGG 0.411176 5:+8385195 None:intergenic
CAACAGGGCAAGTTTGGAAA+AGG 0.416141 5:-8385388 MsG0580024698.01.T01:intron
CATACCTCTCATAATCACTT+AGG 0.429482 5:+8385499 None:intergenic
GTTAGCAACAAGCGTGGAGA+AGG 0.441857 5:-8385355 MsG0580024698.01.T01:CDS
ATACCTCTCATAATCACTTA+GGG 0.455021 5:+8385500 None:intergenic
ATTTCGATCTCACAGCTGAC+AGG 0.485786 5:+8385268 None:intergenic
ATGTGCATATGTTGATTCAC+AGG 0.490249 5:-8385756 MsG0580024698.01.T01:intron
AATTAAAGATCTATATTGCC+AGG 0.499516 5:+8385099 None:intergenic
TGGATCTGCAACACTATCAA+TGG 0.512103 5:-8386122 MsG0580024698.01.T01:intron
GAAATTCCTCAACTCCGTTA+CGG 0.539768 5:+8385535 None:intergenic
GCATGTCTTCGTGCGTTGAA+AGG 0.542596 5:-8385797 MsG0580024698.01.T01:CDS
AAAGTACGACTTGGCCGTAA+CGG 0.543008 5:-8385549 MsG0580024698.01.T01:CDS
TATCACATCCTGAAAATAAG+TGG 0.549427 5:-8385303 MsG0580024698.01.T01:three_prime_UTR
CACATCCTGAAAATAAGTGG+TGG 0.574757 5:-8385300 MsG0580024698.01.T01:three_prime_UTR
TTTGCATCAAAAGTACGACT+TGG 0.583080 5:-8385558 MsG0580024698.01.T01:CDS
TTAGCAACAAGCGTGGAGAA+GGG 0.591626 5:-8385354 MsG0580024698.01.T01:CDS
ACTTGGCCGTAACGGAGTTG+AGG 0.606364 5:-8385541 MsG0580024698.01.T01:CDS
ACATCCTGAAAATAAGTGGT+GGG 0.611234 5:-8385299 MsG0580024698.01.T01:three_prime_UTR
TTTCGATCTCACAGCTGACA+GGG 0.612593 5:+8385269 None:intergenic
GAAAGAGTTAGCAACAAGCG+TGG 0.628742 5:-8385361 MsG0580024698.01.T01:CDS
GTCCCCTAAGTGATTATGAG+AGG 0.633118 5:-8385503 MsG0580024698.01.T01:intron
TCTTCGTGCGTTGAAAGGAG+AGG 0.666069 5:-8385792 MsG0580024698.01.T01:CDS
TAATCACTTAGGGGACCAAG+AGG 0.668806 5:+8385510 None:intergenic
TACCTCTCATAATCACTTAG+GGG 0.681098 5:+8385501 None:intergenic
GGTGGTGAGAGACAGCAGAG+AGG 0.742962 5:-8386514 MsG0580024698.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTTTGAATATAAATAGAT+GGG - Chr5:8385516-8385535 MsG0580024698.01.T01:CDS 10.0%
!!! TTATTTTGAATATAAATAGA+TGG - Chr5:8385515-8385534 MsG0580024698.01.T01:CDS 10.0%
!! TTAATTCCATTAATAAGTAT+AGG + Chr5:8385552-8385571 None:intergenic 15.0%
!!! ATTAGAATCTTTTAAAACTA+TGG + Chr5:8385930-8385949 None:intergenic 15.0%
!!! GAAATAAACACTTTTATTAT+TGG + Chr5:8386453-8386472 None:intergenic 15.0%
!! ACATATAAGAAAAACAATTG+TGG - Chr5:8385608-8385627 MsG0580024698.01.T01:intron 20.0%
!! ACCAAAAAGTAATAAATGAA+TGG - Chr5:8385577-8385596 MsG0580024698.01.T01:CDS 20.0%
!! ATATCTACTTATATTTCAAC+AGG - Chr5:8386203-8386222 MsG0580024698.01.T01:intron 20.0%
!! TATCTACTTATATTTCAACA+GGG - Chr5:8386204-8386223 MsG0580024698.01.T01:intron 20.0%
!! TTATTACACTTATCTAATAC+AGG + Chr5:8386415-8386434 None:intergenic 20.0%
!!! ACCATTCATTTATTACTTTT+TGG + Chr5:8385581-8385600 None:intergenic 20.0%
!!! CTTTAATTTTTCTATATGCA+GGG - Chr5:8385254-8385273 MsG0580024698.01.T01:three_prime_UTR 20.0%
!!! TATATCTTCATTTTTTTTCC+TGG - Chr5:8386490-8386509 MsG0580024698.01.T01:intron 20.0%
!!! TCATTTTTACATGTATCAAT+AGG + Chr5:8386169-8386188 None:intergenic 20.0%
!!! TGAATAAGTACTTTTTCTTA+AGG - Chr5:8385988-8386007 MsG0580024698.01.T01:intron 20.0%
! AATTAAAGATCTATATTGCC+AGG + Chr5:8386511-8386530 None:intergenic 25.0%
! CATATATACTTGTATAGCAT+AGG + Chr5:8385362-8385381 None:intergenic 25.0%
! TTGAAAATACTAATCTGTCT+TGG - Chr5:8385302-8385321 MsG0580024698.01.T01:three_prime_UTR 25.0%
!! AAAACTTTTCCTACAAACAA+AGG + Chr5:8385147-8385166 None:intergenic 25.0%
!! ACAATGTTGTAATTTTCTCT+AGG - Chr5:8385449-8385468 MsG0580024698.01.T01:intron 25.0%
!!! AAATGCTTTGAATTTTGTAG+AGG - Chr5:8385209-8385228 MsG0580024698.01.T01:three_prime_UTR 25.0%
!!! AATCTTTTAAAACTATGGCA+GGG + Chr5:8385925-8385944 None:intergenic 25.0%
!!! CTTTTTTGAAATTTCGTTCT+TGG - Chr5:8385677-8385696 MsG0580024698.01.T01:intron 25.0%
!!! GCTTTAATTTTTCTATATGC+AGG - Chr5:8385253-8385272 MsG0580024698.01.T01:three_prime_UTR 25.0%
AAAATACTAATCTGTCTTGG+TGG - Chr5:8385305-8385324 MsG0580024698.01.T01:three_prime_UTR 30.0%
ATACCTCTCATAATCACTTA+GGG + Chr5:8386110-8386129 None:intergenic 30.0%
ATATGATTCCTTCATTGCTA+TGG - Chr5:8385398-8385417 MsG0580024698.01.T01:intron 30.0%
TAGATAATCCATAGCAATGA+AGG + Chr5:8385409-8385428 None:intergenic 30.0%
TATCACATCCTGAAAATAAG+TGG - Chr5:8386304-8386323 MsG0580024698.01.T01:intron 30.0%
TCATGACTTCTTAACAATGT+TGG - Chr5:8385721-8385740 MsG0580024698.01.T01:intron 30.0%
TGAGAGCCTATACTTATTAA+TGG - Chr5:8385543-8385562 MsG0580024698.01.T01:CDS 30.0%
! GCTATGGATTATCTATACAT+TGG - Chr5:8385414-8385433 MsG0580024698.01.T01:intron 30.0%
!!! GAATCTTTTAAAACTATGGC+AGG + Chr5:8385926-8385945 None:intergenic 30.0%
AATACAGGTTCGCTATTTGA+TGG + Chr5:8386400-8386419 None:intergenic 35.0%
ACATCCTGAAAATAAGTGGT+GGG - Chr5:8386308-8386327 MsG0580024698.01.T01:intron 35.0%
ATATTTCAACAGGGCAAGTT+TGG - Chr5:8386213-8386232 MsG0580024698.01.T01:intron 35.0%
CATACCTCTCATAATCACTT+AGG + Chr5:8386111-8386130 None:intergenic 35.0%
GCTTGCATGGGAAAAAATTT+AGG - Chr5:8385231-8385250 MsG0580024698.01.T01:three_prime_UTR 35.0%
TACCTCTCATAATCACTTAG+GGG + Chr5:8386109-8386128 None:intergenic 35.0%
TCAACTGTTTCATAAAGTGC+AGG - Chr5:8385761-8385780 MsG0580024698.01.T01:CDS 35.0%
TTAAAACTATGGCAGGGAAT+AGG + Chr5:8385919-8385938 None:intergenic 35.0%
! AATTTTGTAGAGGCTTGCAT+GGG - Chr5:8385219-8385238 MsG0580024698.01.T01:three_prime_UTR 35.0%
! ACTTTTCCTACAAACAAAGG+AGG + Chr5:8385144-8385163 None:intergenic 35.0%
! AGGATCTAAGACATCCTTTA+TGG + Chr5:8385899-8385918 None:intergenic 35.0%
! CGTACTTTTGATGCAAAGAA+TGG + Chr5:8386046-8386065 None:intergenic 35.0%
! TTTGCATCAAAAGTACGACT+TGG - Chr5:8386049-8386068 MsG0580024698.01.T01:intron 35.0%
!! AAATCCCACCACTTATTTTC+AGG + Chr5:8386315-8386334 None:intergenic 35.0%
!! ATGTGCATATGTTGATTCAC+AGG - Chr5:8385851-8385870 MsG0580024698.01.T01:intron 35.0%
AAGCATTCAAGCTTCGACTT+GGG + Chr5:8385969-8385988 None:intergenic 40.0%
AGACATCCTTTATGGAAAGC+AGG + Chr5:8385891-8385910 None:intergenic 40.0%
CAAAGTCCTGCTTTCCATAA+AGG - Chr5:8385882-8385901 MsG0580024698.01.T01:intron 40.0%
CACATCCTGAAAATAAGTGG+TGG - Chr5:8386307-8386326 MsG0580024698.01.T01:intron 40.0%
GAAATTCCTCAACTCCGTTA+CGG + Chr5:8386075-8386094 None:intergenic 40.0%
GAGGCTAATTGAATGTGAGT+TGG - Chr5:8385176-8385195 MsG0580024698.01.T01:three_prime_UTR 40.0%
GTTGAGGAATTTCTTCCTCT+TGG - Chr5:8386082-8386101 MsG0580024698.01.T01:intron 40.0%
TCTGTCTTGGTGGAATGTAT+CGG - Chr5:8385315-8385334 MsG0580024698.01.T01:exon 40.0%
TGGATCTGCAACACTATCAA+TGG - Chr5:8385485-8385504 MsG0580024698.01.T01:intron 40.0%
TGTTCAACGACAACTGTTAG+TGG + Chr5:8386137-8386156 None:intergenic 40.0%
! AATTTTCTCTAGGCCAAAGC+TGG - Chr5:8385459-8385478 MsG0580024698.01.T01:intron 40.0%
! GAATTTTGTAGAGGCTTGCA+TGG - Chr5:8385218-8385237 MsG0580024698.01.T01:three_prime_UTR 40.0%
! GCTTGTTGCTAACTCTTTCT+TGG + Chr5:8386245-8386264 None:intergenic 40.0%
!! GCATATGTTGATTCACAGGT+AGG - Chr5:8385855-8385874 MsG0580024698.01.T01:intron 40.0%
!!! GAAAAGTTTTGAGACTCGAG+AGG - Chr5:8385157-8385176 MsG0580024698.01.T01:three_prime_UTR 40.0%
ATTTCGATCTCACAGCTGAC+AGG + Chr5:8386342-8386361 None:intergenic 45.0%
CAACAGGGCAAGTTTGGAAA+AGG - Chr5:8386219-8386238 MsG0580024698.01.T01:intron 45.0%
CAAGCATTCAAGCTTCGACT+TGG + Chr5:8385970-8385989 None:intergenic 45.0%
GAAAGAGTTAGCAACAAGCG+TGG - Chr5:8386246-8386265 MsG0580024698.01.T01:intron 45.0%
GGACTAACAAAGTCTGTCGT+AGG - Chr5:8386009-8386028 MsG0580024698.01.T01:intron 45.0%
GTCCCCTAAGTGATTATGAG+AGG - Chr5:8386104-8386123 MsG0580024698.01.T01:intron 45.0%
TAATCACTTAGGGGACCAAG+AGG + Chr5:8386100-8386119 None:intergenic 45.0%
TTAGCAACAAGCGTGGAGAA+GGG - Chr5:8386253-8386272 MsG0580024698.01.T01:intron 45.0%
TTTCGATCTCACAGCTGACA+GGG + Chr5:8386341-8386360 None:intergenic 45.0%
! AAAGTACGACTTGGCCGTAA+CGG - Chr5:8386058-8386077 MsG0580024698.01.T01:intron 45.0%
! TGCACTCCTCCTTTGTTTGT+AGG - Chr5:8385135-8385154 MsG0580024698.01.T01:three_prime_UTR 45.0%
GCATGTCTTCGTGCGTTGAA+AGG - Chr5:8385810-8385829 MsG0580024698.01.T01:CDS 50.0%
GTTAGCAACAAGCGTGGAGA+AGG - Chr5:8386252-8386271 MsG0580024698.01.T01:intron 50.0%
TCTTCGTGCGTTGAAAGGAG+AGG - Chr5:8385815-8385834 MsG0580024698.01.T01:CDS 50.0%
AGCAGAGAGGCTGGAGTAGT+AGG - Chr5:8385106-8385125 MsG0580024698.01.T01:three_prime_UTR 55.0%
TGCAGATCCAGCTCCAGCTT+TGG + Chr5:8385475-8385494 None:intergenic 55.0%
! ACTTGGCCGTAACGGAGTTG+AGG - Chr5:8386066-8386085 MsG0580024698.01.T01:intron 55.0%
CTCTAGGCCAAAGCTGGAGC+TGG - Chr5:8385465-8385484 MsG0580024698.01.T01:intron 60.0%
GGTGGTGAGAGACAGCAGAG+AGG - Chr5:8385093-8385112 MsG0580024698.01.T01:three_prime_UTR 60.0%
GTGAGAGACAGCAGAGAGGC+TGG - Chr5:8385097-8385116 MsG0580024698.01.T01:three_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
Chr5 gene 8385091 8386538 8385091 ID=MsG0580024698.01;Name=MsG0580024698.01
Chr5 mRNA 8385091 8386538 8385091 ID=MsG0580024698.01.T01;Parent=MsG0580024698.01;Name=MsG0580024698.01.T01;_AED=0.44;_eAED=0.47;_QI=0|0.75|0.8|1|0.5|0.6|5|241|108
Chr5 exon 8385091 8385404 8385091 ID=MsG0580024698.01.T01:exon:2776;Parent=MsG0580024698.01.T01
Chr5 exon 8385504 8385598 8385504 ID=MsG0580024698.01.T01:exon:2775;Parent=MsG0580024698.01.T01
Chr5 exon 8385757 8385846 8385757 ID=MsG0580024698.01.T01:exon:2774;Parent=MsG0580024698.01.T01
Chr5 exon 8386123 8386158 8386123 ID=MsG0580024698.01.T01:exon:2773;Parent=MsG0580024698.01.T01
Chr5 exon 8386506 8386538 8386506 ID=MsG0580024698.01.T01:exon:2772;Parent=MsG0580024698.01.T01
Chr5 CDS 8386506 8386538 8386506 ID=MsG0580024698.01.T01:cds;Parent=MsG0580024698.01.T01
Chr5 CDS 8386123 8386158 8386123 ID=MsG0580024698.01.T01:cds;Parent=MsG0580024698.01.T01
Chr5 CDS 8385757 8385846 8385757 ID=MsG0580024698.01.T01:cds;Parent=MsG0580024698.01.T01
Chr5 CDS 8385504 8385598 8385504 ID=MsG0580024698.01.T01:cds;Parent=MsG0580024698.01.T01
Chr5 CDS 8385332 8385404 8385332 ID=MsG0580024698.01.T01:cds;Parent=MsG0580024698.01.T01
Chr5 three_prime_UTR 8385091 8385331 8385091 ID=MsG0580024698.01.T01:three_prime_utr;Parent=MsG0580024698.01.T01
Gene Sequence

>MsG0580024698.01.T01

ATGGTGGTGAGAGACAGCAGAGAGGCTGGAGTAGCCAAAGCTGGAGCTGGATCTGCAACACTATCAATGGCATATGCTGCTGTTAAATTTGCAGATGCATGTCTTCGTGCGTTGAAAGGAGAGGCTGATATCATTCAATGTGCATATGTTGATTCACAGGTAACTGAATTGCCATTCTTTGCATCAAAAGTACGACTTGGCCGTAACGGAGTTGAGGAATTTCTTCCTCTTGGTCCCCTAAGTGATTATGAGAGGGCAAGTTTGGAAAAGGCCAAGAAAGAGTTAGCAACAAGCGTGGAGAAGGGAGTTTCTTTCATCAGAAAATGA

Protein sequence

>MsG0580024698.01.T01

MVVRDSREAGVAKAGAGSATLSMAYAAVKFADACLRALKGEADIIQCAYVDSQVTELPFFASKVRLGRNGVEEFLPLGPLSDYERASLEKAKKELATSVEKGVSFIRK*