AlfalfaGEDB | MsG0280007695.01

M. sativa cultivar ZhongmuNo.1 / MsG0280007695.01

KEGG: K09338
GO: GO:0003677 GO:0008289
PFAM: PF00046 PF01852 PF08670

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280007695.01.T01	XP_003594520.2	99.282	836	2	1	1	832	1	836	0	1719

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280007695.01.T01	Q6AST1	79.017	834	165	6	8	832	27	859	0	1367

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280007695.01.T01	G7IKU0	99.282	836	2	1	1	832	1	836	0.0	1719

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080047815.01	MsG0280007695.01	0.848340	6.522759e-60	1.193252e-56
MsG0080048258.01	MsG0280007695.01	0.853376	2.488753e-61	5.366346e-58
MsG0080048797.01	MsG0280007695.01	0.852535	4.331317e-61	9.077970e-58
MsG0080048905.01	MsG0280007695.01	0.802371	5.791108e-49	2.832089e-46
MsG0180000984.01	MsG0280007695.01	0.853038	3.111995e-61	6.633825e-58
MsG0180001288.01	MsG0280007695.01	0.813440	2.556437e-51	1.669627e-48
MsG0180001588.01	MsG0280007695.01	0.835577	1.547314e-56	1.899942e-53
MsG0180001719.01	MsG0280007695.01	0.867539	1.270528e-65	4.451900e-62
MsG0180002428.01	MsG0280007695.01	0.807294	5.422677e-50	3.009113e-47
MsG0180003857.01	MsG0280007695.01	0.815938	7.152434e-52	4.999138e-49

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PPI

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Gene1	Gene2	Type
MsG0280007695.01	MsG0480020792.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	bit score
MsG0280007695.01.T01	MTR_2g030130	99.282	836	2	1	1	832	1	836	1719
MsG0280007695.01.T01	MTR_4g058970	69.451	838	237	7	4	832	19	846	1194
MsG0280007695.01.T01	MTR_2g101190	67.467	833	256	9	9	832	10	836	1162
MsG0280007695.01.T01	MTR_3g109800	66.707	832	257	9	9	832	16	835	1154
MsG0280007695.01.T01	MTR_8g013980	66.466	832	261	9	9	832	11	832	1150
MsG0280007695.01.T01	MTR_2g094520	63.377	841	282	9	4	832	13	839	1098
MsG0280007695.01.T01	MTR_3g109800	66.964	784	239	9	9	784	16	787	1079
MsG0280007695.01.T01	MTR_3g109800	67.096	699	214	7	9	701	16	704	964
MsG0280007695.01.T01	MTR_3g109800	62.584	596	211	7	245	832	3	594	782

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280007695.01.T01	AT2G34710	78.427	839	163	10	7	832	19	852	0.0	1352
MsG0280007695.01.T01	AT1G30490	76.576	841	168	11	7	832	15	841	0.0	1325
MsG0280007695.01.T01	AT5G60690	66.982	845	246	11	4	832	15	842	0.0	1147
MsG0280007695.01.T01	AT1G52150	66.228	835	260	13	9	832	13	836	0.0	1128
MsG0280007695.01.T01	AT1G52150	66.268	836	259	14	9	832	13	837	0.0	1126
MsG0280007695.01.T01	AT4G32880	64.586	833	276	12	9	832	11	833	0.0	1109
MsG0280007695.01.T01	AT1G52150	66.074	787	245	13	9	784	13	788	0.0	1051
MsG0280007695.01.T01	AT5G32670	80.000	70	14	0	286	355	37	106	1.32e-31	122
MsG0280007695.01.T01	AT4G04890	30.534	131	62	3	13	122	65	187	2.92e-11	67.8
MsG0280007695.01.T01	AT4G04890	21.569	510	302	19	189	670	297	736	9.68e-11	66.2

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Find 245 sgRNAs with CRISPR-Local

Find 362 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACCAGAAGGCAATAAGA+GGG	0.641093	2:+19357396	None:intergenic
AAACACAATGATGATATCCT+TGG	0.512064	2:+19355956	None:intergenic
AAACGGATACAACCTTAGGT+CGG	0.721957	2:-19357162	MsG0280007695.01.T01:CDS
AAAGGTGTTACTAACCCAGC+TGG	0.505524	2:+19359755	None:intergenic
AAAGTTAAGTATCTTGATTA+TGG	0.305354	2:+19356234	None:intergenic
AAATGATACTTAACTGATTT+AGG	0.211026	2:+19357355	None:intergenic
AACCAAACTTTGATCTGTTT+AGG	0.371881	2:+19360874	None:intergenic
AACCTAAACAGATCAAAGTT+TGG	0.353061	2:-19360876	MsG0280007695.01.T01:CDS
AACTTGTTGCAGCTTTGCAG+TGG	0.607045	2:-19357493	MsG0280007695.01.T01:intron
AAGACACCACCTCCAAATGT+TGG	0.525330	2:+19358101	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAACACAATGATGATATCCT+TGG	+	Chr2:19361284-19361303	None:intergenic	30.0%
AACCAAACTTTGATCTGTTT+AGG	+	Chr2:19356366-19356385	None:intergenic	30.0%
AACCTAAACAGATCAAAGTT+TGG	-	Chr2:19356361-19356380	MsG0280007695.01.T01:CDS	30.0%
AATTACCTGTTGCATAATCT+TGG	+	Chr2:19360731-19360750	None:intergenic	30.0%
AATTTCATTGTTCAACAGCA+AGG	+	Chr2:19357902-19357921	None:intergenic	30.0%
ACTATGTGTCTACATACATA+TGG	+	Chr2:19361252-19361271	None:intergenic	30.0%
ACTGAGATATACTACAAGTT+TGG	-	Chr2:19358227-19358246	MsG0280007695.01.T01:CDS	30.0%
AGAACATTGGATTTACTTGA+AGG	-	Chr2:19360013-19360032	MsG0280007695.01.T01:intron	30.0%
AGATAGAGTGAGTTAGAATT+AGG	+	Chr2:19358957-19358976	None:intergenic	30.0%
AGTGATTCTCAAAGGTAAAT+TGG	+	Chr2:19360117-19360136	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	19355940	19361319	19355940	ID=MsG0280007695.01;Name=MsG0280007695.01
Chr2	mRNA	19355940	19361319	19355940	ID=MsG0280007695.01.T01;Parent=MsG0280007695.01;Name=MsG0280007695.01.T01;_AED=0.22;_eAED=0.22;_QI=261\|0.94\|0.94\|1\|1\|1\|18\|325\|832
Chr2	exon	19355940	19356411	19355940	ID=MsG0280007695.01.T01:exon:4463;Parent=MsG0280007695.01.T01
Chr2	exon	19356515	19356664	19356515	ID=MsG0280007695.01.T01:exon:4462;Parent=MsG0280007695.01.T01
Chr2	exon	19356760	19356859	19356760	ID=MsG0280007695.01.T01:exon:4461;Parent=MsG0280007695.01.T01
Chr2	exon	19356948	19357264	19356948	ID=MsG0280007695.01.T01:exon:4460;Parent=MsG0280007695.01.T01
Chr2	exon	19357369	19357503	19357369	ID=MsG0280007695.01.T01:exon:4459;Parent=MsG0280007695.01.T01
Chr2	exon	19357609	19357689	19357609	ID=MsG0280007695.01.T01:exon:4458;Parent=MsG0280007695.01.T01
Chr2	exon	19357780	19357968	19357780	ID=MsG0280007695.01.T01:exon:4457;Parent=MsG0280007695.01.T01
Chr2	exon	19358073	19358259	19358073	ID=MsG0280007695.01.T01:exon:4456;Parent=MsG0280007695.01.T01
Chr2	exon	19358407	19358513	19358407	ID=MsG0280007695.01.T01:exon:4455;Parent=MsG0280007695.01.T01
Chr2	exon	19358593	19358667	19358593	ID=MsG0280007695.01.T01:exon:4454;Parent=MsG0280007695.01.T01
Chr2	exon	19358754	19358909	19358754	ID=MsG0280007695.01.T01:exon:4453;Parent=MsG0280007695.01.T01
Chr2	exon	19358993	19359076	19358993	ID=MsG0280007695.01.T01:exon:4452;Parent=MsG0280007695.01.T01
Chr2	exon	19359180	19359293	19359180	ID=MsG0280007695.01.T01:exon:4451;Parent=MsG0280007695.01.T01
Chr2	exon	19359388	19359483	19359388	ID=MsG0280007695.01.T01:exon:4450;Parent=MsG0280007695.01.T01
Chr2	exon	19359577	19359667	19359577	ID=MsG0280007695.01.T01:exon:4449;Parent=MsG0280007695.01.T01
Chr2	exon	19359770	19359882	19359770	ID=MsG0280007695.01.T01:exon:4448;Parent=MsG0280007695.01.T01
Chr2	exon	19360039	19360198	19360039	ID=MsG0280007695.01.T01:exon:4447;Parent=MsG0280007695.01.T01
Chr2	exon	19360862	19361319	19360862	ID=MsG0280007695.01.T01:exon:4446;Parent=MsG0280007695.01.T01
Chr2	five_prime_UTR	19361059	19361319	19361059	ID=MsG0280007695.01.T01:five_prime_utr;Parent=MsG0280007695.01.T01
Chr2	CDS	19360862	19361058	19360862	ID=MsG0280007695.01.T01:cds;Parent=MsG0280007695.01.T01
Chr2	CDS	19360039	19360198	19360039	ID=MsG0280007695.01.T01:cds;Parent=MsG0280007695.01.T01
Chr2	CDS	19359770	19359882	19359770	ID=MsG0280007695.01.T01:cds;Parent=MsG0280007695.01.T01
Chr2	CDS	19359577	19359667	19359577	ID=MsG0280007695.01.T01:cds;Parent=MsG0280007695.01.T01
Chr2	CDS	19359388	19359483	19359388	ID=MsG0280007695.01.T01:cds;Parent=MsG0280007695.01.T01
Chr2	CDS	19359180	19359293	19359180	ID=MsG0280007695.01.T01:cds;Parent=MsG0280007695.01.T01
Chr2	CDS	19358993	19359076	19358993	ID=MsG0280007695.01.T01:cds;Parent=MsG0280007695.01.T01
Chr2	CDS	19358754	19358909	19358754	ID=MsG0280007695.01.T01:cds;Parent=MsG0280007695.01.T01
Chr2	CDS	19358593	19358667	19358593	ID=MsG0280007695.01.T01:cds;Parent=MsG0280007695.01.T01
Chr2	CDS	19358407	19358513	19358407	ID=MsG0280007695.01.T01:cds;Parent=MsG0280007695.01.T01
Chr2	CDS	19358073	19358259	19358073	ID=MsG0280007695.01.T01:cds;Parent=MsG0280007695.01.T01
Chr2	CDS	19357780	19357968	19357780	ID=MsG0280007695.01.T01:cds;Parent=MsG0280007695.01.T01
Chr2	CDS	19357609	19357689	19357609	ID=MsG0280007695.01.T01:cds;Parent=MsG0280007695.01.T01
Chr2	CDS	19357369	19357503	19357369	ID=MsG0280007695.01.T01:cds;Parent=MsG0280007695.01.T01
Chr2	CDS	19356948	19357264	19356948	ID=MsG0280007695.01.T01:cds;Parent=MsG0280007695.01.T01
Chr2	CDS	19356760	19356859	19356760	ID=MsG0280007695.01.T01:cds;Parent=MsG0280007695.01.T01
Chr2	CDS	19356515	19356664	19356515	ID=MsG0280007695.01.T01:cds;Parent=MsG0280007695.01.T01
Chr2	CDS	19356265	19356411	19356265	ID=MsG0280007695.01.T01:cds;Parent=MsG0280007695.01.T01
Chr2	three_prime_UTR	19355940	19356264	19355940	ID=MsG0280007695.01.T01:three_prime_utr;Parent=MsG0280007695.01.T01

Gene Sequence

>MsG0280007695.01.T01

ATGGCACTTTCTATGCATAAAGAAATGGATGCTGGTAAATATGTAAGGTACACTCCTGAACAAGTTGAAGCTTTGGAGAGAGTTTATTCAGAATGTCCTAAGCCTAGTTCTTTGAGAAGACAACAACTCATTAGAGAATGTCCTATCCTTTCTAACATTGAACCTAAACAGATCAAAGTTTGGTTTCAAAACCGAAGATGCCGTGAGAAGCAAAGAAAGGAAGCTTCACGTTTACAGACGGTGAATAGGAAGTTGTCTGCAATGAACAAGCTCTTGATGGAAGAGAATGACCGTCTGCAGAAGCAAGTTTCTCATCTGGTGTATGAAAATGGATACATGAAGCAACAGATTCATACTGCTTCTGCTGCAACTACCACAGACAATAGCTGTGATTCTGTGGTAATGAGTGGTCAAAACCAACAGCAAAACCCAACTCAGCGTCCTCAAAGGGATGCAAACAACCCAGCTGGTCTTCTCGCCGTGGCTGAGGAGACCCTGGCAGAGTTCCTATCTAAGGCTACCGGAACTGCTGTCGACTGGGTGCAGATGATTGGGATGAAGCCTGGTCCGGAATCTATTGGTATCGTTGCTGTTTCCCGCAACTCTAGTGGGATAGCGGCGCGAGCCTGCGGCCTTGTGAGTCTAGAGCCCACTAAGGTTGCCGAGATTCTTAAAGATAGACTGTCATGGTATCGTGACTGCCGATGCGTTGATGTATTGAGTATTGTTCCCACTGGTGGTGGTGGTACAATAGAGCTTATGTACATGCAGACGTATGCGCCTACAACTTTGGCTGCAGCACGAGACTTTTGGACACTGAGATATACTACAAGTTTGGAAGATGGAAGTCTTGTGATATGTGAGAGATCGTTGAATGCTTCTACTGGTGGCCCTACAGGGCCTTCTCCGTCAAATTTTGTGAGAGCTGAAATGCTTCCTAGTGGTTTCCTTATTAGACCTTGCGAGGGGGGCGGATCCATTATTCATATTGTTGATCACGTTGATTTAGATGTTTGGAGTGTCCCTGAAGTTCTGAGGCCCCTCTATGAATCGTCAAAAATCTTGGCTCAGAAATTGACTATTGCTGCATTGCAACACATAAAACAGATAGCACAAGAATCCAGTGGAGAAATTCAGTATGGTGGCGGGCGCCAACCAGCTGTTTTGAGAACTTTTAGTCAGAGACTTTGCAGGGGGTTCAATGATGCTGTGAATGGATTTGTCGATGACGGTTGGTCGTTGCTGGGAAATGATGGTGTTGAAGACGTAACAATTGCTGTAAACTCGTCTCCAAACAAGTTTTTGGGGTCCAACTATTCATCAATGTTCCCAACATTTGGAGGTGGTGTCTTGTGTGCCAAGGCATCAATGCTACTGCAGAATGTTCCTCCTGCTTTGCTTGTTCGATTTTTGAGGGAGCATCGATCAGAATGGGCTGATTATGGCGTTGATGCCTACTCTGCTACTTGTCTCAAGTCTAGCCCCTATGCAGTTCCGTGTCCAAGACCCGGTGGCTTTCCCAGCAGCCAGGTCATTTTACCTCTTGCTCCTACGATCGAGCACGAAGAGTTCCTGGAGGTAGTTCGTATAGAGGGACATGCATTCTCCCCAGAAGATGTTGCACTGGCACGTGATATGTATCTTTTGCAGCTTTGCAGTGGAATTGATGAAAATTCAATTGGAGCATGTGCTCAACTTGTGTTTGCCCCTATAGATGAATCCTTTGCAGATGATGCCCTCTTATTGCCTTCTGGTTTTCGAGTTATTCCCTTGGATCCTAAATCAGACGGTCCAACCACATCAAGAACATTGGATTTACTTGAAGGATCTAGTAATGCTCGTCCATCTGGTGAAGGTGCTGGTTCAAACGGATACAACCTTAGGTCGGTCCTTACAATTGCATTCCAATTTACCTTTGAGAATCACTTGCGCGACAATGTGGCTTCCATGGCTCGCCAGTATGTGCGTAGTGTTGTGGCATCAGTTCAGAGGGTTGCCATGGCAATTGCGCCCTCTCGGCCAGGTACTCAGCTTGGGCCAAAGTCTCTTCCTGGTTCGCCAGAAGCTCATGCTTTGGCACGATGGATATCTAGAAGCTACAGGATGCACACTGGTGCCGAGCTTTTTAGAGTTGAGTCCGCAGCGAGTGATGCAATATTGAAACAACTTTGGCACCATTCTGATGCAATTATGTGCTGTTCTGCATCTCCAATATTCACCTTTTCAAACCAGGCTGGACTAGACATGCTTGAAACTACTCTAGTTGCTCTTCAAGATATTATGCTTGATAAAGTTCTTGATGAAGCTGGCAGGAAGATTCTTTGCTCTGAGTTCTCCAAGATTATGCAACAGGGTTATGCATCTCTGCCAGGAGGGATTTGTGTATCAAGTATGAATAGGCCAGTGTCTTATGAGCAAGCCATTGCATGGAAAGTTCTTAATGATGATGATGCCAATCACTGCTTAGCTTTCATGTTCATCAACTGGTCCTTTGTCTGA

Protein sequence

>MsG0280007695.01.T01

MALSMHKEMDAGKYVRYTPEQVEALERVYSECPKPSSLRRQQLIRECPILSNIEPKQIKVWFQNRRCREKQRKEASRLQTVNRKLSAMNKLLMEENDRLQKQVSHLVYENGYMKQQIHTASAATTTDNSCDSVVMSGQNQQQNPTQRPQRDANNPAGLLAVAEETLAEFLSKATGTAVDWVQMIGMKPGPESIGIVAVSRNSSGIAARACGLVSLEPTKVAEILKDRLSWYRDCRCVDVLSIVPTGGGGTIELMYMQTYAPTTLAAARDFWTLRYTTSLEDGSLVICERSLNASTGGPTGPSPSNFVRAEMLPSGFLIRPCEGGGSIIHIVDHVDLDVWSVPEVLRPLYESSKILAQKLTIAALQHIKQIAQESSGEIQYGGGRQPAVLRTFSQRLCRGFNDAVNGFVDDGWSLLGNDGVEDVTIAVNSSPNKFLGSNYSSMFPTFGGGVLCAKASMLLQNVPPALLVRFLREHRSEWADYGVDAYSATCLKSSPYAVPCPRPGGFPSSQVILPLAPTIEHEEFLEVVRIEGHAFSPEDVALARDMYLLQLCSGIDENSIGACAQLVFAPIDESFADDALLLPSGFRVIPLDPKSDGPTTSRTLDLLEGSSNARPSGEGAGSNGYNLRSVLTIAFQFTFENHLRDNVASMARQYVRSVVASVQRVAMAIAPSRPGTQLGPKSLPGSPEAHALARWISRSYRMHTGAELFRVESAASDAILKQLWHHSDAIMCCSASPIFTFSNQAGLDMLETTLVALQDIMLDKVLDEAGRKILCSEFSKIMQQGYASLPGGICVSSMNRPVSYEQAIAWKVLNDDDANHCLAFMFINWSFV*