AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680032005.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680032005.01.T01 MTR_2g021040 94.366 71 4 0 23 93 42 112 1.62e-43 143
MsG0680032005.01.T01 MTR_2g021040 94.366 71 4 0 23 93 42 112 3.54e-43 143
MsG0680032005.01.T01 MTR_2g021040 94.366 71 4 0 23 93 42 112 6.75e-43 142
MsG0680032005.01.T01 MTR_0003s0650 73.973 73 17 2 23 93 37 109 4.26e-29 107
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680032005.01.T01 AT5G18940 68.000 75 23 1 23 97 42 115 1.84e-29 108
MsG0680032005.01.T01 AT5G18940 68.000 75 23 1 23 97 42 115 1.92e-29 107

Find 31 sgRNAs with CRISPR-Local

Find 84 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACAATGAGAACTATGCTTTC+TGG 0.269593 6:+31804208 MsG0680032005.01.T01:CDS
CGTGTCAAGAGCGGGGTTCT+TGG 0.312982 6:+31804620 MsG0680032005.01.T01:three_prime_UTR
TCTTGAACAATACTATAGTA+TGG 0.338598 6:+31804768 MsG0680032005.01.T01:three_prime_UTR
ATTCACAAGTTGCCTATACT+TGG 0.363293 6:+31804313 MsG0680032005.01.T01:CDS
TACTTTATTGTAGGCATTAG+AGG 0.369327 6:+31804165 MsG0680032005.01.T01:intron
CCTTCTAATCGCACTATGTA+TGG 0.370960 6:+31804818 MsG0680032005.01.T01:three_prime_UTR
CCATACATAGTGCGATTAGA+AGG 0.384710 6:-31804818 None:intergenic
TCAAGAAAGTTAGTAAAAGA+AGG 0.425433 6:-31804751 None:intergenic
CTTACTTCCCATCCAAGTAT+AGG 0.450135 6:-31804325 None:intergenic
ATCAGAACCGAATTTAGATC+TGG 0.456620 6:+31804240 MsG0680032005.01.T01:CDS
CAAGAAATCGTGTCAAGAGC+GGG 0.461491 6:+31804612 MsG0680032005.01.T01:three_prime_UTR
AGAACTATGCTTTCTGGAGA+TGG 0.464715 6:+31804214 MsG0680032005.01.T01:CDS
TGTGAAACCAGATCTAAATT+CGG 0.477833 6:-31804247 None:intergenic
TGCAGTAGATTCAGCTTCAC+TGG 0.486587 6:+31803328 MsG0680032005.01.T01:CDS
TACTAATTGAAATGTTGTGC+AGG 0.493586 6:+31803273 None:intergenic
ACAAGTTGCCTATACTTGGA+TGG 0.498032 6:+31804317 MsG0680032005.01.T01:CDS
GAAAGGATTTAGTCCACTGT+TGG 0.511448 6:+31804431 MsG0680032005.01.T01:CDS
AGATCTGGTTTCACAGCTTG+TGG 0.516259 6:+31804255 MsG0680032005.01.T01:CDS
CAAGTTGCCTATACTTGGAT+GGG 0.523597 6:+31804318 MsG0680032005.01.T01:CDS
ATTTGATGATCTTCATCATG+TGG 0.570148 6:-31804531 None:intergenic
CTCGTATCACTCTTCACCCA+CGG 0.572581 6:-31804647 None:intergenic
GAGTGAACACGGTTGATGCT+TGG 0.608303 6:-31804574 None:intergenic
CGGGGTTCTTGGAACACCGT+GGG 0.615525 6:+31804631 MsG0680032005.01.T01:three_prime_UTR
TCTGGTTTCACAGCTTGTGG+AGG 0.620384 6:+31804258 MsG0680032005.01.T01:CDS
TCAAGAAATCGTGTCAAGAG+CGG 0.632895 6:+31804611 MsG0680032005.01.T01:three_prime_UTR
AAGAAATCGTGTCAAGAGCG+GGG 0.665713 6:+31804613 MsG0680032005.01.T01:three_prime_UTR
GCTTGTGGAGGAAATCTGCA+AGG 0.666944 6:+31804270 MsG0680032005.01.T01:CDS
TCAACAATATCGTCCAACAG+TGG 0.680311 6:-31804444 None:intergenic
GCGGGGTTCTTGGAACACCG+TGG 0.682296 6:+31804630 MsG0680032005.01.T01:three_prime_UTR
CGTGGGTGAAGAGTGATACG+AGG 0.709427 6:+31804648 MsG0680032005.01.T01:three_prime_UTR
TGTGGAGGAAATCTGCAAGG+AGG 0.743747 6:+31804273 MsG0680032005.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAATTACTCATAAATATA+TGG + Chr6:31803869-31803888 MsG0680032005.01.T01:intron 10.0%
!!! ATACATATCATTACTATTTT+TGG + Chr6:31803427-31803446 MsG0680032005.01.T01:intron 15.0%
!!! TACATATCATTACTATTTTT+GGG + Chr6:31803428-31803447 MsG0680032005.01.T01:intron 15.0%
!! AAATATTGTACATAAAAGCA+AGG - Chr6:31803679-31803698 None:intergenic 20.0%
!!! AACACTATCAAGATTTTTAT+TGG - Chr6:31803739-31803758 None:intergenic 20.0%
!!! ATTTCACTTCTTTTCATTTT+GGG + Chr6:31803394-31803413 MsG0680032005.01.T01:intron 20.0%
!!! TAGTTATTTTTAGTTTGTGA+AGG + Chr6:31804499-31804518 MsG0680032005.01.T01:exon 20.0%
! AATATATGGAAAATGCTTGT+TGG + Chr6:31803883-31803902 MsG0680032005.01.T01:intron 25.0%
! CTGCAATAAAGTGATAATAA+CGG - Chr6:31803373-31803392 None:intergenic 25.0%
! GAAGAATAAAAATAGTGTAG+TGG + Chr6:31804670-31804689 MsG0680032005.01.T01:three_prime_UTR 25.0%
! TCAAGAAAGTTAGTAAAAGA+AGG - Chr6:31804754-31804773 None:intergenic 25.0%
! TCTTGAACAATACTATAGTA+TGG + Chr6:31804768-31804787 MsG0680032005.01.T01:three_prime_UTR 25.0%
! TGCAATAAAGTGATAATAAC+GGG - Chr6:31803372-31803391 None:intergenic 25.0%
! TTGTTGAAGTCTGAAAAATT+TGG + Chr6:31804460-31804479 MsG0680032005.01.T01:CDS 25.0%
!! CATTTCACTTCTTTTCATTT+TGG + Chr6:31803393-31803412 MsG0680032005.01.T01:intron 25.0%
!! TGCATTTTACAACTTAATGT+TGG + Chr6:31803506-31803525 MsG0680032005.01.T01:intron 25.0%
!!! ATTTTTAGTTTGTGAAGGTT+CGG + Chr6:31804504-31804523 MsG0680032005.01.T01:three_prime_UTR 25.0%
!!! TGCTTGTTTTACTTTATTGT+AGG + Chr6:31804156-31804175 MsG0680032005.01.T01:intron 25.0%
ATATCACTGGAACATATCTA+TGG - Chr6:31804086-31804105 None:intergenic 30.0%
TACTTTATTGTAGGCATTAG+AGG + Chr6:31804165-31804184 MsG0680032005.01.T01:intron 30.0%
TCTTGATCTATCAGTACTAT+TGG - Chr6:31803768-31803787 None:intergenic 30.0%
TGTGAAACCAGATCTAAATT+CGG - Chr6:31804250-31804269 None:intergenic 30.0%
TTAACAAAAGCTCAGATTCT+TGG - Chr6:31803614-31803633 None:intergenic 30.0%
! AAAGAAAGGCATAATATCAC+TGG - Chr6:31804099-31804118 None:intergenic 30.0%
! AAGTCTGAAAAATTTGGTGA+AGG + Chr6:31804466-31804485 MsG0680032005.01.T01:CDS 30.0%
! ATTTGATGATCTTCATCATG+TGG - Chr6:31804534-31804553 None:intergenic 30.0%
!! AATTTTGATACAGGCTAGAA+AGG + Chr6:31804414-31804433 MsG0680032005.01.T01:intron 30.0%
!! GAGTAATTTTAGTGCTTGTT+TGG - Chr6:31803860-31803879 None:intergenic 30.0%
!!! TTTTTAGTTTGTGAAGGTTC+GGG + Chr6:31804505-31804524 MsG0680032005.01.T01:three_prime_UTR 30.0%
ACAATGAGAACTATGCTTTC+TGG + Chr6:31804208-31804227 MsG0680032005.01.T01:CDS 35.0%
AGCAACTGCAAGTAAAAGAA+AGG - Chr6:31804113-31804132 None:intergenic 35.0%
ATCAGAACCGAATTTAGATC+TGG + Chr6:31804240-31804259 MsG0680032005.01.T01:CDS 35.0%
ATTCACAAGTTGCCTATACT+TGG + Chr6:31804313-31804332 MsG0680032005.01.T01:CDS 35.0%
TCTATCAGTACTATTGGCAT+TGG - Chr6:31803762-31803781 None:intergenic 35.0%
! GTCTTTTATTCGAGTGAACA+CGG - Chr6:31804588-31804607 None:intergenic 35.0%
!! TCGCACTATGTATGGTTTTA+TGG + Chr6:31804826-31804845 MsG0680032005.01.T01:three_prime_UTR 35.0%
!! TCTTTCAAGTTAATACAGCG+TGG + Chr6:31803833-31803852 MsG0680032005.01.T01:intron 35.0%
ACAAGTTGCCTATACTTGGA+TGG + Chr6:31804317-31804336 MsG0680032005.01.T01:CDS 40.0%
ACCCATCTAAAAAGACCAGA+GGG - Chr6:31803653-31803672 None:intergenic 40.0%
ATGTGTCATCAGTTTGTCGT+GGG + Chr6:31804013-31804032 MsG0680032005.01.T01:intron 40.0%
CAAGTTGCCTATACTTGGAT+GGG + Chr6:31804318-31804337 MsG0680032005.01.T01:CDS 40.0%
CCATACATAGTGCGATTAGA+AGG - Chr6:31804821-31804840 None:intergenic 40.0%
CCTTCTAATCGCACTATGTA+TGG + Chr6:31804818-31804837 MsG0680032005.01.T01:three_prime_UTR 40.0%
CTTACTTCCCATCCAAGTAT+AGG - Chr6:31804328-31804347 None:intergenic 40.0%
GAAAGGATTTAGTCCACTGT+TGG + Chr6:31804431-31804450 MsG0680032005.01.T01:CDS 40.0%
GATCAAGACTCAGATTCTGA+TGG + Chr6:31803780-31803799 MsG0680032005.01.T01:intron 40.0%
TACCCATCTAAAAAGACCAG+AGG - Chr6:31803654-31803673 None:intergenic 40.0%
TACTCCTTATATGTACCCTC+TGG + Chr6:31803635-31803654 MsG0680032005.01.T01:intron 40.0%
TACTTGCAGTTGCTCTACAT+TGG + Chr6:31804119-31804138 MsG0680032005.01.T01:intron 40.0%
TATGTGTCATCAGTTTGTCG+TGG + Chr6:31804012-31804031 MsG0680032005.01.T01:intron 40.0%
TCAACAATATCGTCCAACAG+TGG - Chr6:31804447-31804466 None:intergenic 40.0%
TCAAGAAATCGTGTCAAGAG+CGG + Chr6:31804611-31804630 MsG0680032005.01.T01:three_prime_UTR 40.0%
! AAGACCAGAGGGTACATATA+AGG - Chr6:31803642-31803661 None:intergenic 40.0%
! AAGTGCCGTGTTTCATCTAA+GGG - Chr6:31803955-31803974 None:intergenic 40.0%
! AGAACTATGCTTTCTGGAGA+TGG + Chr6:31804214-31804233 MsG0680032005.01.T01:CDS 40.0%
! AGTTGCTCTACATTGGTTAC+TGG + Chr6:31804126-31804145 MsG0680032005.01.T01:intron 40.0%
! ATGCATGCAGTTGACTTAAC+TGG + Chr6:31804371-31804390 MsG0680032005.01.T01:intron 40.0%
! TTTGTGCTGGTGTAAGATAG+TGG + Chr6:31803563-31803582 MsG0680032005.01.T01:intron 40.0%
!! AAAGTGCCGTGTTTCATCTA+AGG - Chr6:31803956-31803975 None:intergenic 40.0%
!! ACCCTCTGGTCTTTTTAGAT+GGG + Chr6:31803649-31803668 MsG0680032005.01.T01:intron 40.0%
!! GGGTGCATGAATTTTGATAC+AGG + Chr6:31804405-31804424 MsG0680032005.01.T01:intron 40.0%
!! TACCCTCTGGTCTTTTTAGA+TGG + Chr6:31803648-31803667 MsG0680032005.01.T01:intron 40.0%
AAGAAATCGTGTCAAGAGCG+GGG + Chr6:31804613-31804632 MsG0680032005.01.T01:three_prime_UTR 45.0%
ACTTAACTGGTTGCTCATCG+TGG + Chr6:31804384-31804403 MsG0680032005.01.T01:intron 45.0%
AGCTGTGGTGTAAAGCAGTA+AGG - Chr6:31803462-31803481 None:intergenic 45.0%
CAAGAAATCGTGTCAAGAGC+GGG + Chr6:31804612-31804631 MsG0680032005.01.T01:three_prime_UTR 45.0%
CTAATAAGCTCGACTAGCGA+CGG + Chr6:31803914-31803933 MsG0680032005.01.T01:intron 45.0%
CTTAACTGGTTGCTCATCGT+GGG + Chr6:31804385-31804404 MsG0680032005.01.T01:intron 45.0%
GTCTGTGTCTAAGTTTGTGC+TGG + Chr6:31803550-31803569 MsG0680032005.01.T01:intron 45.0%
TGCAGTAGATTCAGCTTCAC+TGG + Chr6:31803328-31803347 MsG0680032005.01.T01:CDS 45.0%
! AGATCTGGTTTCACAGCTTG+TGG + Chr6:31804255-31804274 MsG0680032005.01.T01:CDS 45.0%
CTCGTATCACTCTTCACCCA+CGG - Chr6:31804650-31804669 None:intergenic 50.0%
GCCGACCCTTAGATGAAACA+CGG + Chr6:31803947-31803966 MsG0680032005.01.T01:intron 50.0%
GCCGTGTTTCATCTAAGGGT+CGG - Chr6:31803951-31803970 None:intergenic 50.0%
GCTTGTGGAGGAAATCTGCA+AGG + Chr6:31804270-31804289 MsG0680032005.01.T01:CDS 50.0%
TGTGGAGGAAATCTGCAAGG+AGG + Chr6:31804273-31804292 MsG0680032005.01.T01:CDS 50.0%
! GAGTGAACACGGTTGATGCT+TGG - Chr6:31804577-31804596 None:intergenic 50.0%
! TCTGGTTTCACAGCTTGTGG+AGG + Chr6:31804258-31804277 MsG0680032005.01.T01:CDS 50.0%
CGTGGGTGAAGAGTGATACG+AGG + Chr6:31804648-31804667 MsG0680032005.01.T01:three_prime_UTR 55.0%
GCACACGAGCTACACATGTG+TGG - Chr6:31803489-31803508 None:intergenic 55.0%
! CACATGTGTGGTGTCAGCTG+TGG - Chr6:31803477-31803496 None:intergenic 55.0%
CGGGGTTCTTGGAACACCGT+GGG + Chr6:31804631-31804650 MsG0680032005.01.T01:three_prime_UTR 60.0%
CGTGTCAAGAGCGGGGTTCT+TGG + Chr6:31804620-31804639 MsG0680032005.01.T01:three_prime_UTR 60.0%
GCGGGGTTCTTGGAACACCG+TGG + Chr6:31804630-31804649 MsG0680032005.01.T01:three_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
Chr6 gene 31803284 31804852 31803284 ID=MsG0680032005.01;Name=MsG0680032005.01
Chr6 mRNA 31803284 31804852 31803284 ID=MsG0680032005.01.T01;Parent=MsG0680032005.01;Name=MsG0680032005.01.T01;_AED=0.41;_eAED=0.42;_QI=0|1|0.66|1|1|1|3|351|101
Chr6 exon 31803284 31803349 31803284 ID=MsG0680032005.01.T01:exon:15562;Parent=MsG0680032005.01.T01
Chr6 exon 31804178 31804342 31804178 ID=MsG0680032005.01.T01:exon:15563;Parent=MsG0680032005.01.T01
Chr6 exon 31804427 31804852 31804427 ID=MsG0680032005.01.T01:exon:15564;Parent=MsG0680032005.01.T01
Chr6 CDS 31803284 31803349 31803284 ID=MsG0680032005.01.T01:cds;Parent=MsG0680032005.01.T01
Chr6 CDS 31804178 31804342 31804178 ID=MsG0680032005.01.T01:cds;Parent=MsG0680032005.01.T01
Chr6 CDS 31804427 31804501 31804427 ID=MsG0680032005.01.T01:cds;Parent=MsG0680032005.01.T01
Chr6 three_prime_UTR 31804502 31804852 31804502 ID=MsG0680032005.01.T01:three_prime_utr;Parent=MsG0680032005.01.T01
Gene Sequence

>MsG0680032005.01.T01

ATGTTGTGCAGGAACATTGTATATGCTTCTTCTTATGACTTGAATGCAGTAGATTCAGCTTCACTGGCATTAGAGGAAGTTGAAAAGAATTTCGTAACAATGAGAACTATGCTTTCTGGAGATGGAGAATCAGAACCGAATTTAGATCTGGTTTCACAGCTTGTGGAGGAAATCTGCAAGGAGGATGTTCTTACTCTTCTAATTCACAAGTTGCCTATACTTGGATGGGAAGCTAGAAAGGATTTAGTCCACTGTTGGACGATATTGTTGAAGTCTGAAAAATTTGGTGAAGGATTGCATTCGTAG

Protein sequence

>MsG0680032005.01.T01

MLCRNIVYASSYDLNAVDSASLALEEVEKNFVTMRTMLSGDGESEPNLDLVSQLVEEICKEDVLTLLIHKLPILGWEARKDLVHCWTILLKSEKFGEGLHS*