AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480019206.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480019206.01.T01 MTR_8g104280 55.963 109 17 2 1 109 636 713 2.17e-28 109
MsG0480019206.01.T01 MTR_2g099870 52.294 109 21 2 1 109 530 607 1.66e-25 101
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480019206.01.T01 AT4G10760 44.231 104 27 2 1 104 603 675 5.63e-20 85.5

Find 27 sgRNAs with CRISPR-Local

Find 51 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGTTGTGGTTGTAGCTTTA+GGG 0.297042 4:-16171229 MsG0480019206.01.T01:intron
GCTGGCTGCAACTCCACATA+TGG 0.404331 4:+16171039 None:intergenic
GCTTCAGATTTCTATGCCTA+TGG 0.422704 4:+16171193 None:intergenic
ATATTCTCCATTGTTCCATC+AGG 0.428820 4:-16171140 MsG0480019206.01.T01:CDS
TCCATCAGGTGGTTGTCTCC+TGG 0.437714 4:-16171126 MsG0480019206.01.T01:CDS
TTCTCCATTGTTCCATCAGG+TGG 0.456120 4:-16171137 MsG0480019206.01.T01:CDS
TTACACTACTCGACTGTGAA+TGG 0.479973 4:+16171468 None:intergenic
GGACTTGTCCTCTCGAACAA+AGG 0.481391 4:+16171520 None:intergenic
TACACTACTCGACTGTGAAT+GGG 0.490671 4:+16171469 None:intergenic
GACTCTAGTGTTGTCAAACA+AGG 0.496365 4:-16170985 MsG0480019206.01.T01:CDS
GGTGTAAGGTTAGTTGATGA+AGG 0.503650 4:-16171090 MsG0480019206.01.T01:CDS
GTAATCAATTGAGTGGTGTA+AGG 0.514327 4:-16171104 MsG0480019206.01.T01:CDS
TTGATGAAGGGCTGCTGGCA+AGG 0.514978 4:-16171077 MsG0480019206.01.T01:CDS
ACCTCTTAGAGCTTCTGCTA+TGG 0.518633 4:-16171013 MsG0480019206.01.T01:CDS
ACACCACTCAATTGATTACC+AGG 0.521151 4:+16171108 None:intergenic
TAAAGCAGCTTATCCATATG+TGG 0.537056 4:-16171052 MsG0480019206.01.T01:CDS
TCCATAGCAGAAGCTCTAAG+AGG 0.537539 4:+16171012 None:intergenic
GTTAGTTGATGAAGGGCTGC+TGG 0.539111 4:-16171082 MsG0480019206.01.T01:CDS
GAAGCTCTAAGAGGTGATGC+TGG 0.557595 4:+16171021 None:intergenic
GCAGCACTTCGATTCTGTGC+CGG 0.570481 4:+16170952 None:intergenic
ACCAGGAGACAACCACCTGA+TGG 0.584066 4:+16171125 None:intergenic
AGATGTATCCTTTGTTCGAG+AGG 0.592364 4:-16171528 MsG0480019206.01.T01:CDS
ACAACCACCTGATGGAACAA+TGG 0.620773 4:+16171133 None:intergenic
CTCTAGTGTTGTCAAACAAG+GGG 0.639545 4:-16170983 MsG0480019206.01.T01:CDS
TCTCCTGGTAATCAATTGAG+TGG 0.640738 4:-16171111 MsG0480019206.01.T01:CDS
ACTCTAGTGTTGTCAAACAA+GGG 0.641402 4:-16170984 MsG0480019206.01.T01:CDS
GTGTAAGGTTAGTTGATGAA+GGG 0.665822 4:-16171089 MsG0480019206.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCTTAAAAGTTAAAATAGTT+TGG - Chr4:16171054-16171073 MsG0480019206.01.T01:CDS 15.0%
!!! TTTAACTTTTAAGAATACAA+AGG + Chr4:16171048-16171067 None:intergenic 15.0%
!!! TTTATTTTAATGTTTTCACA+TGG - Chr4:16171501-16171520 MsG0480019206.01.T01:CDS 15.0%
!! AAAAGTTAAAATAGTTTGGT+TGG - Chr4:16171058-16171077 MsG0480019206.01.T01:CDS 20.0%
!! AAAGTTAAAATAGTTTGGTT+GGG - Chr4:16171059-16171078 MsG0480019206.01.T01:CDS 20.0%
! AAGTTAAAATAGTTTGGTTG+GGG - Chr4:16171060-16171079 MsG0480019206.01.T01:CDS 25.0%
TTTCAAATCCAGACAATACT+CGG + Chr4:16171086-16171105 None:intergenic 30.0%
!!! AAATCTGAAGCTGTTTTTTG+AGG - Chr4:16171224-16171243 MsG0480019206.01.T01:intron 30.0%
!!! AATTCCAGCTTTCTTGTTTT+TGG + Chr4:16170909-16170928 None:intergenic 30.0%
AAGTCCAAAAACAAGAAAGC+TGG - Chr4:16170902-16170921 MsG0480019206.01.T01:CDS 35.0%
ACTCTAGTGTTGTCAAACAA+GGG - Chr4:16171421-16171440 MsG0480019206.01.T01:intron 35.0%
ATATTCTCCATTGTTCCATC+AGG - Chr4:16171265-16171284 MsG0480019206.01.T01:intron 35.0%
CTTTGACGAAGAACCTAATT+TGG - Chr4:16171156-16171175 MsG0480019206.01.T01:CDS 35.0%
GTAATCAATTGAGTGGTGTA+AGG - Chr4:16171301-16171320 MsG0480019206.01.T01:intron 35.0%
TAAAGCAGCTTATCCATATG+TGG - Chr4:16171353-16171372 MsG0480019206.01.T01:intron 35.0%
! AGGTTTTCAATTCTCATGAC+TGG - Chr4:16171002-16171021 MsG0480019206.01.T01:CDS 35.0%
!! GTGTAAGGTTAGTTGATGAA+GGG - Chr4:16171316-16171335 MsG0480019206.01.T01:intron 35.0%
ACACCACTCAATTGATTACC+AGG + Chr4:16171300-16171319 None:intergenic 40.0%
CGAAGAACCTAATTTGGTTG+TGG - Chr4:16171162-16171181 MsG0480019206.01.T01:CDS 40.0%
CTCTAGTGTTGTCAAACAAG+GGG - Chr4:16171422-16171441 MsG0480019206.01.T01:intron 40.0%
GACTCTAGTGTTGTCAAACA+AGG - Chr4:16171420-16171439 MsG0480019206.01.T01:intron 40.0%
GCTACAACCACAACCAAATT+AGG + Chr4:16171172-16171191 None:intergenic 40.0%
GCTTCAGATTTCTATGCCTA+TGG + Chr4:16171215-16171234 None:intergenic 40.0%
GGGCAGAAAAAATCATCCAT+AGG - Chr4:16171196-16171215 MsG0480019206.01.T01:CDS 40.0%
TACACTACTCGACTGTGAAT+GGG + Chr4:16170939-16170958 None:intergenic 40.0%
TCTCCTGGTAATCAATTGAG+TGG - Chr4:16171294-16171313 MsG0480019206.01.T01:intron 40.0%
TTACACTACTCGACTGTGAA+TGG + Chr4:16170940-16170959 None:intergenic 40.0%
TTGGTTGTGGTTGTAGCTTT+AGG - Chr4:16171175-16171194 MsG0480019206.01.T01:CDS 40.0%
! TGGTTGTGGTTGTAGCTTTA+GGG - Chr4:16171176-16171195 MsG0480019206.01.T01:CDS 40.0%
!! GGTGTAAGGTTAGTTGATGA+AGG - Chr4:16171315-16171334 MsG0480019206.01.T01:intron 40.0%
ACAACCACCTGATGGAACAA+TGG + Chr4:16171275-16171294 None:intergenic 45.0%
ACCTCTTAGAGCTTCTGCTA+TGG - Chr4:16171392-16171411 MsG0480019206.01.T01:intron 45.0%
CATGCACACATACTCATGCA+GGG - Chr4:16170978-16170997 MsG0480019206.01.T01:CDS 45.0%
CTGCATGAGTATGTGTGCAT+GGG + Chr4:16170979-16170998 None:intergenic 45.0%
TTCTCCATTGTTCCATCAGG+TGG - Chr4:16171268-16171287 MsG0480019206.01.T01:intron 45.0%
!! CAAACAAGGGGTCGATTTTC+CGG - Chr4:16171434-16171453 MsG0480019206.01.T01:intron 45.0%
!! TCCATAGCAGAAGCTCTAAG+AGG + Chr4:16171396-16171415 None:intergenic 45.0%
CACACATACTCATGCAGGGT+AGG - Chr4:16170982-16171001 MsG0480019206.01.T01:CDS 50.0%
CACAGTCGAGTAGTGTAAGC+TGG - Chr4:16170942-16170961 MsG0480019206.01.T01:CDS 50.0%
CCATGCACACATACTCATGC+AGG - Chr4:16170977-16170996 MsG0480019206.01.T01:CDS 50.0%
CCTGCATGAGTATGTGTGCA+TGG + Chr4:16170980-16170999 None:intergenic 50.0%
GGACTTGTCCTCTCGAACAA+AGG + Chr4:16170888-16170907 None:intergenic 50.0%
TGAGTATGTGTGCATGGGAG+TGG + Chr4:16170974-16170993 None:intergenic 50.0%
!! GAAGCTCTAAGAGGTGATGC+TGG + Chr4:16171387-16171406 None:intergenic 50.0%
!! GTTAGTTGATGAAGGGCTGC+TGG - Chr4:16171323-16171342 MsG0480019206.01.T01:intron 50.0%
ACCAGGAGACAACCACCTGA+TGG + Chr4:16171283-16171302 None:intergenic 55.0%
GCAGCACTTCGATTCTGTGC+CGG + Chr4:16171456-16171475 None:intergenic 55.0%
GCTGGCTGCAACTCCACATA+TGG + Chr4:16171369-16171388 None:intergenic 55.0%
TCCATCAGGTGGTTGTCTCC+TGG - Chr4:16171279-16171298 MsG0480019206.01.T01:intron 55.0%
TTGATGAAGGGCTGCTGGCA+AGG - Chr4:16171328-16171347 MsG0480019206.01.T01:intron 55.0%
! GGTTGGGGCCGAGTATTGTC+TGG - Chr4:16171075-16171094 MsG0480019206.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr4 gene 16170879 16171548 16170879 ID=MsG0480019206.01;Name=MsG0480019206.01
Chr4 mRNA 16170879 16171548 16170879 ID=MsG0480019206.01.T01;Parent=MsG0480019206.01;Name=MsG0480019206.01.T01;_AED=0.49;_eAED=0.63;_QI=0|0|0|1|0|0|2|0|144
Chr4 exon 16171472 16171548 16171472 ID=MsG0480019206.01.T01:exon:5214;Parent=MsG0480019206.01.T01
Chr4 exon 16170879 16171236 16170879 ID=MsG0480019206.01.T01:exon:5213;Parent=MsG0480019206.01.T01
Chr4 CDS 16171472 16171548 16171472 ID=MsG0480019206.01.T01:cds;Parent=MsG0480019206.01.T01
Chr4 CDS 16170879 16171236 16170879 ID=MsG0480019206.01.T01:cds;Parent=MsG0480019206.01.T01
Gene Sequence

>MsG0480019206.01.T01

ATGTATCCTTTGTTCGAGAGGACAAGTCCAAAAACAAGAAAGCTGGAATTGTTTGCTCCCATTCACAGTCGAGTAGTCTTTAGGGCAGAAAAAATCATCCATAGGCATAGAAATCTGAAGCTGTTTTTTGAGGTGTTCTTTATTATTGATTATATTCTCCATTGTTCCATCAGGTGGTTGTCTCCTGGTAATCAATTGAGTGGTGTAAGGTTAGTTGATGAAGGGCTGCTGGCAAGGTTTAAAGCAGCTTATCCATATGTGGAGTTGCAGCCAGCATCACCTCTTAGAGCTTCTGCTATGGAAGTAGACTCTAGTGTTGTCAAACAAGGGGTCGATTTTCCGGCACAGAATCGAAGTGCTGCATTCAAGTTGTTCTTGAGAGCTATATTTATTTTAATGTTTTCACATGGTCATCAGTGCTGCATGACACGTTGA

Protein sequence

>MsG0480019206.01.T01

MYPLFERTSPKTRKLELFAPIHSRVVFRAEKIIHRHRNLKLFFEVFFIIDYILHCSIRWLSPGNQLSGVRLVDEGLLARFKAAYPYVELQPASPLRASAMEVDSSVVKQGVDFPAQNRSAAFKLFLRAIFILMFSHGHQCCMTR*