AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580027183.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580027183.01.T01 MTR_6g032880 80.435 138 22 1 48 185 254 386 9.44e-71 219
MsG0580027183.01.T01 MTR_6g032880 70.000 70 21 0 30 99 171 240 4.29e-23 94.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580027183.01.T01 AT1G55370 36.449 214 102 4 3 184 142 353 4.49e-36 129

Find 35 sgRNAs with CRISPR-Local

Find 115 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAACATGATATTATATATT+TGG 0.110960 5:-55718762 None:intergenic
GAACCTTGCAACGGTCATTT+TGG 0.155770 5:+55716622 MsG0580027183.01.T01:CDS
TCTTGTGGACTTAATACTAT+TGG 0.306506 5:-55718708 None:intergenic
TGGGAAAGATTTGAAGGCTT+TGG 0.332452 5:+55716642 MsG0580027183.01.T01:CDS
TATATTTGGATTGTGTAGAT+AGG 0.365670 5:-55718748 None:intergenic
TCAGTATTAGTTGAAAGAAA+TGG 0.369710 5:+55718588 MsG0580027183.01.T01:CDS
TTTAATCCAATCATTTCATA+TGG 0.389242 5:-55717847 None:intergenic
AGATATGAGTGGTGAGGAAA+TGG 0.407819 5:+55718179 MsG0580027183.01.T01:CDS
AAAGGACCATATGAAATGAT+TGG 0.428403 5:+55717841 MsG0580027183.01.T01:CDS
CTGTTCAGTCCAGGCTCATC+AGG 0.435338 5:+55718627 MsG0580027183.01.T01:CDS
TATTAAGTCCACAAGATGTA+TGG 0.449867 5:+55718715 MsG0580027183.01.T01:CDS
CCTCCTTTCCATACATCTTG+TGG 0.476094 5:-55718723 None:intergenic
CTGATGAGCCTGGACTGAAC+AGG 0.479462 5:-55718626 None:intergenic
TCTTTCCCTCTTCAAATGAC+AGG 0.486076 5:+55717931 MsG0580027183.01.T01:CDS
TCGATCAGTGTAAAACTTCG+AGG 0.495155 5:-55718252 None:intergenic
GTGGACTTAATACTATTGGT+TGG 0.500437 5:-55718704 None:intergenic
TACTGTCAAATGACTCAAGA+TGG 0.504674 5:-55717956 None:intergenic
CTTTCAATTCCTGATGAGCC+TGG 0.508570 5:-55718636 None:intergenic
ATTAAAATACATTGTGACAT+TGG 0.516339 5:+55717864 MsG0580027183.01.T01:CDS
ATTGTGACATTGGAAGAAGA+TGG 0.536047 5:+55717874 MsG0580027183.01.T01:CDS
ACATTGGAAGAAGATGGATT+AGG 0.545100 5:+55717880 MsG0580027183.01.T01:CDS
GACTTAATACTATTGGTTGG+AGG 0.550217 5:-55718701 None:intergenic
GAAACATACCTGTTCAGTCC+AGG 0.552112 5:+55718618 MsG0580027183.01.T01:CDS
TTCCCAAAATGACCGTTGCA+AGG 0.563787 5:-55716625 None:intergenic
ATGGAACCTGTCATTTGAAG+AGG 0.572878 5:-55717937 None:intergenic
AAAGCAATGCAGAAGACTAG+TGG 0.573246 5:+55716600 None:intergenic
TAGTGAAGATATGAGTGGTG+AGG 0.575265 5:+55718173 MsG0580027183.01.T01:CDS
TGGAACCTGTCATTTGAAGA+GGG 0.598388 5:-55717936 None:intergenic
GAGGATAGTGAAGATATGAG+TGG 0.603114 5:+55718168 MsG0580027183.01.T01:CDS
TAATCAGGACAATGAAGCAG+AGG 0.615604 5:+55718149 MsG0580027183.01.T01:intron
AAGTATGCTTACATATGTGT+TGG 0.616114 5:+55718666 MsG0580027183.01.T01:CDS
AGTCCACAAGATGTATGGAA+AGG 0.619952 5:+55718720 MsG0580027183.01.T01:CDS
CCACAAGATGTATGGAAAGG+AGG 0.629918 5:+55718723 MsG0580027183.01.T01:CDS
CAGGTAGTATTATTAAACAA+AGG 0.634880 5:+55717823 MsG0580027183.01.T01:intron
AGACTAGTGGAACCTTGCAA+CGG 0.678469 5:+55716613 MsG0580027183.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAATTAAAGTTGTATTGA+TGG + Chr5:55717657-55717676 MsG0580027183.01.T01:intron 15.0%
!! TTAAGATGTTTATTCATAAA+TGG - Chr5:55718340-55718359 None:intergenic 15.0%
!! AAATTGTGAGAAAAAACATA+AGG - Chr5:55717758-55717777 None:intergenic 20.0%
!! ATTAAAATACATTGTGACAT+TGG + Chr5:55717864-55717883 MsG0580027183.01.T01:CDS 20.0%
!! TAAGATTAAAACAAAGTCAA+TGG + Chr5:55718376-55718395 MsG0580027183.01.T01:intron 20.0%
!! TTTAATCCAATCATTTCATA+TGG - Chr5:55717850-55717869 None:intergenic 20.0%
!!! ATGTAATTTTGATCAATGAA+GGG + Chr5:55718483-55718502 MsG0580027183.01.T01:intron 20.0%
!!! CGTTTGTTTTTTTTTTATCA+GGG + Chr5:55718555-55718574 MsG0580027183.01.T01:intron 20.0%
!!! CTTACACATTCTTATTATTT+TGG + Chr5:55717614-55717633 MsG0580027183.01.T01:intron 20.0%
!!! TATGTAATTTTGATCAATGA+AGG + Chr5:55718482-55718501 MsG0580027183.01.T01:intron 20.0%
!!! TCGTTTGTTTTTTTTTTATC+AGG + Chr5:55718554-55718573 MsG0580027183.01.T01:intron 20.0%
! AAAATAAACTCCGATTCAAT+AGG - Chr5:55718042-55718061 None:intergenic 25.0%
! AGAAAGGATTTGGAAAAATA+TGG + Chr5:55718085-55718104 MsG0580027183.01.T01:intron 25.0%
! CAAAGATGTTCCTTTATAAT+GGG + Chr5:55716789-55716808 MsG0580027183.01.T01:intron 25.0%
! CAGGTAGTATTATTAAACAA+AGG + Chr5:55717823-55717842 MsG0580027183.01.T01:intron 25.0%
! TATATTTGGATTGTGTAGAT+AGG - Chr5:55718751-55718770 None:intergenic 25.0%
! TCAGTATTAGTTGAAAGAAA+TGG + Chr5:55718588-55718607 MsG0580027183.01.T01:CDS 25.0%
!! ATTTCAAATTGGTTCTTCAT+AGG + Chr5:55716817-55716836 MsG0580027183.01.T01:intron 25.0%
!!! CACATTCTTATTATTTTGGT+TGG + Chr5:55717618-55717637 MsG0580027183.01.T01:intron 25.0%
!!! TAAGCAATTTTAACCAAGTT+GGG + Chr5:55718119-55718138 MsG0580027183.01.T01:intron 25.0%
!!! TTAAGCAATTTTAACCAAGT+TGG + Chr5:55718118-55718137 MsG0580027183.01.T01:intron 25.0%
AAAGGACCATATGAAATGAT+TGG + Chr5:55717841-55717860 MsG0580027183.01.T01:CDS 30.0%
AACACTTGATTTACAATCAG+AGG - Chr5:55717121-55717140 None:intergenic 30.0%
AAGACACAAAGCAATTATAC+TGG - Chr5:55717380-55717399 None:intergenic 30.0%
AAGTATGCTTACATATGTGT+TGG + Chr5:55718666-55718685 MsG0580027183.01.T01:CDS 30.0%
AAGTTGAGAAAAACACAAGA+AGG - Chr5:55717539-55717558 None:intergenic 30.0%
ACATTGAAGATATCTTATGC+AGG + Chr5:55717804-55717823 MsG0580027183.01.T01:intron 30.0%
AGTTGAGAAAAACACAAGAA+GGG - Chr5:55717538-55717557 None:intergenic 30.0%
ATACACTTGCACATATACTT+AGG + Chr5:55718314-55718333 MsG0580027183.01.T01:intron 30.0%
CAAATTAGGCAAAAGATCAT+TGG + Chr5:55716848-55716867 MsG0580027183.01.T01:intron 30.0%
GAAATCCATTCCCATTATAA+AGG - Chr5:55716802-55716821 None:intergenic 30.0%
GCAAAGATGTTCCTTTATAA+TGG + Chr5:55716788-55716807 MsG0580027183.01.T01:intron 30.0%
TATTAAGTCCACAAGATGTA+TGG + Chr5:55718715-55718734 MsG0580027183.01.T01:CDS 30.0%
TCTTGTGGACTTAATACTAT+TGG - Chr5:55718711-55718730 None:intergenic 30.0%
TCTTTCACACCTCTATATAA+AGG + Chr5:55716927-55716946 MsG0580027183.01.T01:intron 30.0%
TTATAAAGGAACATCTTTGC+AGG - Chr5:55716788-55716807 None:intergenic 30.0%
! AATGGGAATGGATTTCAAAT+TGG + Chr5:55716806-55716825 MsG0580027183.01.T01:intron 30.0%
! ATGTTCCTTTATAATGGGAA+TGG + Chr5:55716794-55716813 MsG0580027183.01.T01:intron 30.0%
! CAATGATCTTTTGCCTAATT+TGG - Chr5:55716850-55716869 None:intergenic 30.0%
!! AAGCAATTTTAACCAAGTTG+GGG + Chr5:55718120-55718139 MsG0580027183.01.T01:intron 30.0%
!! TCATTTTGGGAAAGATTTGA+AGG + Chr5:55716636-55716655 MsG0580027183.01.T01:CDS 30.0%
!!! CTTATTATTTTGGTTGGCAA+AGG + Chr5:55717624-55717643 MsG0580027183.01.T01:intron 30.0%
AAGTTGGGGAACAAATAATC+AGG + Chr5:55718134-55718153 MsG0580027183.01.T01:intron 35.0%
ACATTCTAGTGAAATCTCCT+TGG + Chr5:55717312-55717331 MsG0580027183.01.T01:intron 35.0%
ATAAACTCCGATTCAATAGG+CGG - Chr5:55718039-55718058 None:intergenic 35.0%
ATTGTGACATTGGAAGAAGA+TGG + Chr5:55717874-55717893 MsG0580027183.01.T01:CDS 35.0%
CATAGGACAACAACCAAATT+AGG + Chr5:55716834-55716853 MsG0580027183.01.T01:intron 35.0%
CTGTCTCTTGAGGTTATAAA+AGG + Chr5:55717714-55717733 MsG0580027183.01.T01:intron 35.0%
GATTTAAACTCTGTCTCTTG+AGG + Chr5:55717704-55717723 MsG0580027183.01.T01:intron 35.0%
GTCTTCACTCCTTTATATAG+AGG - Chr5:55716939-55716958 None:intergenic 35.0%
GTGGACTTAATACTATTGGT+TGG - Chr5:55718707-55718726 None:intergenic 35.0%
GTTGAGAAAAACACAAGAAG+GGG - Chr5:55717537-55717556 None:intergenic 35.0%
TACTGTCAAATGACTCAAGA+TGG - Chr5:55717959-55717978 None:intergenic 35.0%
TATCGTTAAACCTATCGTTG+AGG - Chr5:55718297-55718316 None:intergenic 35.0%
TGATTATTTGTTCCCCAACT+TGG - Chr5:55718135-55718154 None:intergenic 35.0%
TTGAGAAAAACACAAGAAGG+GGG - Chr5:55717536-55717555 None:intergenic 35.0%
TTGATTGTGTTCTTGAGCAT+AGG + Chr5:55717217-55717236 MsG0580027183.01.T01:intron 35.0%
! AAGTTTTACACTGATCGACA+GGG + Chr5:55718257-55718276 MsG0580027183.01.T01:CDS 35.0%
! ACAAAACAAAGTAGTGGAGA+AGG - Chr5:55716721-55716740 None:intergenic 35.0%
! ACATTGGAAGAAGATGGATT+AGG + Chr5:55717880-55717899 MsG0580027183.01.T01:CDS 35.0%
! GACTTAATACTATTGGTTGG+AGG - Chr5:55718704-55718723 None:intergenic 35.0%
!! CAGATAAGACTCTAAGACTT+AGG - Chr5:55717082-55717101 None:intergenic 35.0%
ACACACTATCTCACACATCT+AGG + Chr5:55718512-55718531 MsG0580027183.01.T01:intron 40.0%
ACGAGCAAAGAGAAAGGATT+TGG + Chr5:55718075-55718094 MsG0580027183.01.T01:intron 40.0%
AGATATGAGTGGTGAGGAAA+TGG + Chr5:55718179-55718198 MsG0580027183.01.T01:CDS 40.0%
AGTCCACAAGATGTATGGAA+AGG + Chr5:55718720-55718739 MsG0580027183.01.T01:CDS 40.0%
ATGCAGAACAATTTCAGCAG+AGG - Chr5:55717017-55717036 None:intergenic 40.0%
CCAGAAGCAACTTATGCAAT+AGG + Chr5:55717985-55718004 MsG0580027183.01.T01:intron 40.0%
CCTATTGCATAAGTTGCTTC+TGG - Chr5:55717988-55718007 None:intergenic 40.0%
CCTTTCTCTTTGCTCGTATT+CGG - Chr5:55718072-55718091 None:intergenic 40.0%
GAACAATTTCAGCAGAGGTT+TGG - Chr5:55717012-55717031 None:intergenic 40.0%
GAGGATAGTGAAGATATGAG+TGG + Chr5:55718168-55718187 MsG0580027183.01.T01:CDS 40.0%
GGAGTGAAGACATAGAGATT+TGG + Chr5:55716948-55716967 MsG0580027183.01.T01:intron 40.0%
GTGGACACAAAACAAAGTAG+TGG - Chr5:55716727-55716746 None:intergenic 40.0%
TAATCAGAATCTGAACGCAG+CGG - Chr5:55717429-55717448 None:intergenic 40.0%
TAATCAGGACAATGAAGCAG+AGG + Chr5:55718149-55718168 MsG0580027183.01.T01:intron 40.0%
TAATCGTCATCCTCAACGAT+AGG + Chr5:55718284-55718303 MsG0580027183.01.T01:intron 40.0%
TAGTGAAGATATGAGTGGTG+AGG + Chr5:55718173-55718192 MsG0580027183.01.T01:CDS 40.0%
TCGATCAGTGTAAAACTTCG+AGG - Chr5:55718255-55718274 None:intergenic 40.0%
TCTTTCCCTCTTCAAATGAC+AGG + Chr5:55717931-55717950 MsG0580027183.01.T01:CDS 40.0%
TGAGAAAAACACAAGAAGGG+GGG - Chr5:55717535-55717554 None:intergenic 40.0%
TGGGAAAGATTTGAAGGCTT+TGG + Chr5:55716642-55716661 MsG0580027183.01.T01:CDS 40.0%
TGTAAAGTGTGCTTGAGCAA+AGG + Chr5:55717248-55717267 MsG0580027183.01.T01:intron 40.0%
TTCTCCTAAACTGAACACAC+TGG - Chr5:55717484-55717503 None:intergenic 40.0%
! AAACAAAGTAGTGGAGAAGG+TGG - Chr5:55716718-55716737 None:intergenic 40.0%
! ATGGAACCTGTCATTTGAAG+AGG - Chr5:55717940-55717959 None:intergenic 40.0%
! GAAGTTTTACACTGATCGAC+AGG + Chr5:55718256-55718275 MsG0580027183.01.T01:CDS 40.0%
! TGGAACCTGTCATTTGAAGA+GGG - Chr5:55717939-55717958 None:intergenic 40.0%
! TGTTTTCTTGTCTTGTAGCG+TGG - Chr5:55716746-55716765 None:intergenic 40.0%
!! AACCTTGCAACGGTCATTTT+GGG + Chr5:55716623-55716642 MsG0580027183.01.T01:CDS 40.0%
!! TCGCTCTTTTAACTGCTTGT+AGG - Chr5:55718208-55718227 None:intergenic 40.0%
AAGTGGGATTGGACTACTTC+CGG + Chr5:55717341-55717360 MsG0580027183.01.T01:intron 45.0%
AGACTAGTGGAACCTTGCAA+CGG + Chr5:55716613-55716632 MsG0580027183.01.T01:CDS 45.0%
ATTGGACTACTTCCGGATTG+TGG + Chr5:55717348-55717367 MsG0580027183.01.T01:intron 45.0%
CCACAAGATGTATGGAAAGG+AGG + Chr5:55718723-55718742 MsG0580027183.01.T01:CDS 45.0%
CCGAATACGAGCAAAGAGAA+AGG + Chr5:55718069-55718088 MsG0580027183.01.T01:intron 45.0%
CCTCCTTTCCATACATCTTG+TGG - Chr5:55718726-55718745 None:intergenic 45.0%
CTGACCAGTGTGTTCAGTTT+AGG + Chr5:55717477-55717496 MsG0580027183.01.T01:intron 45.0%
CTTTCAATTCCTGATGAGCC+TGG - Chr5:55718639-55718658 None:intergenic 45.0%
GAAACATACCTGTTCAGTCC+AGG + Chr5:55718618-55718637 MsG0580027183.01.T01:CDS 45.0%
TAGCCAACCGCCTATTGAAT+CGG + Chr5:55718029-55718048 MsG0580027183.01.T01:intron 45.0%
TTCCCAAAATGACCGTTGCA+AGG - Chr5:55716628-55716647 None:intergenic 45.0%
TTCTCTTTGCTCGTATTCGG+AGG - Chr5:55718069-55718088 None:intergenic 45.0%
! AATCTCCTTGGAAGTGCAAG+TGG + Chr5:55717324-55717343 MsG0580027183.01.T01:intron 45.0%
! ATCTCCTTGGAAGTGCAAGT+GGG + Chr5:55717325-55717344 MsG0580027183.01.T01:intron 45.0%
! CAACGACTCTTTTGATGTGC+TGG + Chr5:55716889-55716908 MsG0580027183.01.T01:intron 45.0%
! GAACCTTGCAACGGTCATTT+TGG + Chr5:55716622-55716641 MsG0580027183.01.T01:CDS 45.0%
!! TACTGGTTCCTTCCACAATC+CGG - Chr5:55717363-55717382 None:intergenic 45.0%
ACTCCGATTCAATAGGCGGT+TGG - Chr5:55718035-55718054 None:intergenic 50.0%
CAATCCCACTTGCACTTCCA+AGG - Chr5:55717332-55717351 None:intergenic 50.0%
GACTACTTCCGGATTGTGGA+AGG + Chr5:55717352-55717371 MsG0580027183.01.T01:intron 50.0%
TTGCAGGCGTAGCTGTATTC+TGG - Chr5:55716772-55716791 None:intergenic 50.0%
! CTTGGAAGTGCAAGTGGGAT+TGG + Chr5:55717330-55717349 MsG0580027183.01.T01:intron 50.0%
CTGATGAGCCTGGACTGAAC+AGG - Chr5:55718629-55718648 None:intergenic 55.0%
CTGTTCAGTCCAGGCTCATC+AGG + Chr5:55718627-55718646 MsG0580027183.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr5 gene 55716606 55718773 55716606 ID=MsG0580027183.01;Name=MsG0580027183.01
Chr5 mRNA 55716606 55718773 55716606 ID=MsG0580027183.01.T01;Parent=MsG0580027183.01;Name=MsG0580027183.01.T01;_AED=0.49;_eAED=0.57;_QI=0|0|0|1|1|1|4|0|185
Chr5 exon 55716606 55716692 55716606 ID=MsG0580027183.01.T01:exon:41688;Parent=MsG0580027183.01.T01
Chr5 exon 55717826 55717975 55717826 ID=MsG0580027183.01.T01:exon:41689;Parent=MsG0580027183.01.T01
Chr5 exon 55718156 55718278 55718156 ID=MsG0580027183.01.T01:exon:41690;Parent=MsG0580027183.01.T01
Chr5 exon 55718576 55718773 55718576 ID=MsG0580027183.01.T01:exon:41691;Parent=MsG0580027183.01.T01
Chr5 CDS 55716606 55716692 55716606 ID=MsG0580027183.01.T01:cds;Parent=MsG0580027183.01.T01
Chr5 CDS 55717826 55717975 55717826 ID=MsG0580027183.01.T01:cds;Parent=MsG0580027183.01.T01
Chr5 CDS 55718156 55718278 55718156 ID=MsG0580027183.01.T01:cds;Parent=MsG0580027183.01.T01
Chr5 CDS 55718576 55718773 55718576 ID=MsG0580027183.01.T01:cds;Parent=MsG0580027183.01.T01
Gene Sequence

>MsG0580027183.01.T01

ATGCAGAAGACTAGTGGAACCTTGCAACGGTCATTTTGGGAAAGATTTGAAGGCTTTGGAAGCTTATCTTCAAAGAGCGTTGACATTGTAGTATTATTAAACAAAGGACCATATGAAATGATTGGATTAAAATACATTGTGACATTGGAAGAAGATGGATTAGGCTCAGAGCTTGAAATCTCAAACTCAAACTCTTTCCCTCTTCAAATGACAGGTTCCATCTTGAGTCATTTGACAGACAATGAAGCAGAGGATAGTGAAGATATGAGTGGTGAGGAAATGGATACCTACAAGCAGTTAAAAGAGCGAATAAGTCTTGTATACACGAATGCGCCTCGAAGTTTTACACTGATCGACAGGGGTAGAAGAAACTCAGTATTAGTTGAAAGAAATGGATTTGATGAAACATACCTGTTCAGTCCAGGCTCATCAGGAATTGAAAGTTACAGCAAGTATGCTTACATATGTGTTGGTCAAGCTGTTGTCCTCCAACCAATAGTATTAAGTCCACAAGATGTATGGAAAGGAGGAGCCTATCTACACAATCCAAATATATAA

Protein sequence

>MsG0580027183.01.T01

MQKTSGTLQRSFWERFEGFGSLSSKSVDIVVLLNKGPYEMIGLKYIVTLEEDGLGSELEISNSNSFPLQMTGSILSHLTDNEAEDSEDMSGEEMDTYKQLKERISLVYTNAPRSFTLIDRGRRNSVLVERNGFDETYLFSPGSSGIESYSKYAYICVGQAVVLQPIVLSPQDVWKGGAYLHNPNI*