AlfalfaGEDB | Msa1311550

M. sativa cultivar zhongmu-4 / Msa1311550

KEGG: -
GO: -
PFAM: PF01582.23

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1311550	sp\|V9M398\|RUN1_VITRO	38.339	879	490	16	19	859	27	891	1.11e-157	508

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1311550	G7LIX4	74.790	1190	257	10	16	1172	24	1203	0.0	1731

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0006700	Msa1311550	0.802237	6.172475e-49	-8.615850e-47
Msa0014790	Msa1311550	0.835509	1.609691e-56	-8.615850e-47
Msa0017420	Msa1311550	0.807355	5.264424e-50	-8.615850e-47
Msa0021980	Msa1311550	0.826145	3.184656e-54	-8.615850e-47
Msa0026120	Msa1311550	0.820834	5.563556e-53	-8.615850e-47
Msa0028280	Msa1311550	0.803149	4.001677e-49	-8.615850e-47
Msa0030030	Msa1311550	0.819346	1.219120e-52	-8.615850e-47
Msa0032290	Msa1311550	0.813062	3.095617e-51	-8.615850e-47
Msa0043460	Msa1311550	0.818481	1.917117e-52	-8.615850e-47
Msa0046670	Msa1311550	0.801526	8.637572e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1311550	MtrunA17_Chr8g0342911	74.790	1190	257	10	16	1172	24	1203	0.0	1731
Msa1311550	MtrunA17_Chr8g0351551	69.641	1199	328	9	1	1172	1	1190	0.0	1609
Msa1311550	MtrunA17_Chr8g0353001	77.302	934	205	5	9	936	4	936	0.0	1408
Msa1311550	MtrunA17_Chr8g0342131	82.861	776	128	2	17	787	16	791	0.0	1296
Msa1311550	MtrunA17_Chr8g0372681	71.262	856	241	4	5	855	2	857	0.0	1194
Msa1311550	MtrunA17_Chr8g0342201	87.846	650	74	3	17	661	49	698	0.0	1167
Msa1311550	MtrunA17_Chr4g0020591	41.516	1108	597	18	17	1093	15	1102	0.0	704
Msa1311550	MtrunA17_Chr8g0342221	65.378	569	150	6	628	1172	172	717	0.0	689
Msa1311550	MtrunA17_Chr8g0342221	80.645	155	25	3	16	165	17	171	7.25e-73	257
Msa1311550	MtrunA17_Chr4g0018431	38.124	1162	584	29	14	1134	16	1083	0.0	648

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1311550	AT5G17680.2	36.694	853	517	13	15	860	14	850	5.13e-160	511
Msa1311550	AT5G17680.1	36.577	853	517	14	15	860	14	849	2.06e-157	504
Msa1311550	AT5G18360.1	34.562	868	515	18	18	870	15	844	1.50e-136	437
Msa1311550	AT5G40910.1	33.260	905	531	15	11	915	4	835	3.04e-136	442
Msa1311550	AT5G40910.9	33.260	905	531	15	11	915	4	835	3.04e-136	442
Msa1311550	AT5G22690.3	37.432	740	435	13	19	738	10	741	2.93e-135	433
Msa1311550	AT5G17970.1	36.399	761	460	12	19	762	16	769	3.58e-135	430
Msa1311550	AT5G22690.4	37.432	740	435	13	19	738	10	741	6.32e-135	433
Msa1311550	AT5G46450.3	35.648	864	515	18	19	870	13	847	1.64e-134	437
Msa1311550	AT5G46450.1	35.648	864	515	18	19	870	13	847	4.18e-134	437

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Find 184 sgRNAs with CRISPR-Local

Find 392 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACTGTTTGGTATGAATG+TGG	0.551515	8_4:+8344014	None:intergenic
AAAACTTTGGAATGGAGTAG+TGG	0.603400	8_4:-8345830	Msa1311550:intron
AAAATACAAACTCAAATCAA+CGG	0.625934	8_4:+8344562	None:intergenic
AAAATCTGAGATGTATTCTA+TGG	0.273596	8_4:-8345936	Msa1311550:CDS
AAACATGAACCTCTCCGAGT+CGG	0.600944	8_4:+8344201	None:intergenic
AAACTCTAATGATGATAGTA+AGG	0.449099	8_4:+8344280	None:intergenic
AAAGTTGTGTAGAAGAGTAA+TGG	0.534968	8_4:+8343868	None:intergenic
AAATCTATAAGCTTTCCACA+CGG	0.722019	8_4:-8345976	Msa1311550:CDS
AAATCTGAGATGTATTCTAT+GGG	0.590614	8_4:-8345935	Msa1311550:CDS
AACAAAGTCTCACCTAAGGA+TGG	0.557282	8_4:-8346698	Msa1311550:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TCGAAAAAGGAAATGAAGTT+TGG	-	chr8_4:8343303-8343322	Msa1311550:intron	30.0%
CGAAAAAGGAAATGAAGTTT+GGG	-	chr8_4:8343304-8343323	Msa1311550:intron	30.0%
AGATTAGAAAAACCTGTATG+TGG	+	chr8_4:8343624-8343643	None:intergenic	30.0%
ATTAATTAGGGAACCATGAT+CGG	+	chr8_4:8343836-8343855	None:intergenic	30.0%
TTACAGTGGTTTGAATTTGA+AGG	-	chr8_4:8344088-8344107	Msa1311550:CDS	30.0%
TTCTGTGTTATGATGAAATC+CGG	+	chr8_4:8344407-8344426	None:intergenic	30.0%
TAAGAGACTATGAAGTATGT+CGG	-	chr8_4:8344496-8344515	Msa1311550:CDS	30.0%
AGGGAAATAGATGTGTTAAT+TGG	+	chr8_4:8344694-8344713	None:intergenic	30.0%
TAGCAAGATCTTTAATACTG+CGG	-	chr8_4:8344752-8344771	Msa1311550:CDS	30.0%
TGAAATATGTGGTATGTACA+CGG	+	chr8_4:8344896-8344915	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_4	gene	8343140	8349650	8343140	ID=Msa1311550;Name=Msa1311550
chr8_4	mRNA	8343140	8349650	8343140	ID=Msa1311550-mRNA-1;Parent=Msa1311550;Name=Msa1311550-mRNA-1;_AED=0.42;_eAED=0.42;_QI=0\|0\|0\|0.2\|1\|1\|5\|0\|1172
chr8_4	exon	8349160	8349650	8349160	ID=Msa1311550-mRNA-1:exon:3103;Parent=Msa1311550-mRNA-1
chr8_4	exon	8346226	8347321	8346226	ID=Msa1311550-mRNA-1:exon:3102;Parent=Msa1311550-mRNA-1
chr8_4	exon	8345831	8346124	8345831	ID=Msa1311550-mRNA-1:exon:3101;Parent=Msa1311550-mRNA-1
chr8_4	exon	8343324	8344867	8343324	ID=Msa1311550-mRNA-1:exon:3100;Parent=Msa1311550-mRNA-1
chr8_4	exon	8343140	8343233	8343140	ID=Msa1311550-mRNA-1:exon:3099;Parent=Msa1311550-mRNA-1
chr8_4	CDS	8349160	8349650	8349160	ID=Msa1311550-mRNA-1:cds;Parent=Msa1311550-mRNA-1
chr8_4	CDS	8346226	8347321	8346226	ID=Msa1311550-mRNA-1:cds;Parent=Msa1311550-mRNA-1
chr8_4	CDS	8345831	8346124	8345831	ID=Msa1311550-mRNA-1:cds;Parent=Msa1311550-mRNA-1
chr8_4	CDS	8343324	8344867	8343324	ID=Msa1311550-mRNA-1:cds;Parent=Msa1311550-mRNA-1
chr8_4	CDS	8343140	8343233	8343140	ID=Msa1311550-mRNA-1:cds;Parent=Msa1311550-mRNA-1

Gene Sequence

>Msa1311550

ATGGATATGGCTTCTTCTTCTTCTTCTTCTTCTAACGACGCTACTCTTAAGAAATATGATGTTTTCATCAGCTTTCGAGGCGAGGACACCCGCGTTGGTTTCACTAGTCATCTTTACGCTGCTTTGTGCCGAAGCAGCTTACATACCTACATCGACAACAGGATCGAAAAAGGAAATGAAGTTTGGGTAGAACTTGTGAAAGCAATCAAAGAATCCACTGTGTTTATTGTTGTGTTCTCAGAGAACTATGCGTCTTCAACATGGTGTTTGAACGAACTCGTTCAAATAATGGAATGCCACAAAAATGAACAAATTGTTGTTATTCCTGTGTTCTACCATGTTGACCCTTCACATGTGCGGAAGCAGACTGGGAGTTATGGCACTGCTTTGGCTAAACACAAGAAGCAAGGGAACGACCGTGAGATACAAAATTGGAAGAATGCTCTTTTCCAAGTTGCTAATTTGTCTGGTTTTCATTTAACCACATACAGGACTGAATCCAACTTAATTGAAGACATTACCAGAGCTGTAATAAGAAAATTAAATCAACAAAGCACAATTGATCTTACTTGTAATTTCATACCTGATGAAAACTTTTGGAGCATTCAATCTTTAATAAAATTTGACTCAACAGAAGTTCAAATCATTGGACTTTGGGGTATGGGAGGCATTGGTAAGACAACCCTTGCCACTGCTATGTTTCAAAGGGTCTCTTTCAAATATGATGGCAGTTGCTTCTTTGAAAAGGTGACAGAAGTATCAAAAAGAAATGGAATCAATTACACATGCAACAAACTTCTTTCTAAGTTACTAAAAGAAGATCTTGATATTGACACTCCCAAACTAATACCATCCATGATCATAAGAAGACTCAAAAGCATGAAATCTTTCATCGTACTAGATGATGTACATAATTCAGAACTTCTGCAAAACTTGATTGGAGTGGGACATGGTTGGCTAGGATCCGGTAGCACAGTCATTGTGACTACTAGGGATAAGCATGTGCTGATGAGGGGAGGAATTGAAAAAATTTATGAAGTTACGAAGATGAACTCCAGAAATTCCCTCCGACTTTTCAGTTTCAACGCTTTTAACAAAGTCTCACCTAAGGATGGATATGTGGAGCTCTCAAAAAGAGCAATTGATTATGCTAGAGGAAACCCTTTAGCTTTGAAAGTATTGGGATCATTACTTAGATGTAAAAGTGAAATAGAATGGGATTGTGCATTAGCTAAACTGAAAAAAATGCCAAACAATGAAATTGATTCTATACTCAGAATGAGTTTTAATGAATTGGAAGATACAGAGAAAAATATATTTTTGGATATTGCATGTTTTTTCAAAGGAAGTAAAAGGGAATGGATAACAAAAATATTAAATGAGTGCGGTTTCTTTGCTGATATAGGGATAAGTAATCTTTTAGACAAGGCTCTTGTAAGGGTTGACTCTGAAAATTGCATACAAATGCATTGCTTGATACAGGAAATGGGAAAACAAATTGTTCGTGAAGAATCACCTAAGAATCCCAAACAACGCAGTAGATTATGTGATCCTAAGGAAGTTTATGATGTATTGAAAAATAATAGAGGATCTGAGAAAGTTGAAGCCATATTTTTAGATGCTACTGAATACACACATGTAACTTTAAGACCCGATGCATTTGAAAAGATGAAAAATCTAAGGTTACTTGCTGTTCAAGACCACAAAGGAGTTAAATCTATAAGCTTTCCACACGGTCTTGGTTTATTGCCAGAAAATCTGAGATGTATTCTATGGGATGGCTATCCATTGAAAACTCTCCCTCTAACCTCCTGTCTTGAAATGCTTGTGGAGCTTTCCTTGAAAAGAAGCCATGTGGAAAAACTTTGGAATGGAGTAGTGAATTTACCCAATTTAGAGATAATTGACCTCAGTTGGTCCAAAAAGCTGATAGAGTGTCCAAACGTGTCTGGTTCTCCGAATCTAAAAGAAGTAATACTTAGAGATTGTGAAAGCATGCTTGAAGTTGATTCATCAATTTTCCTTCTCCAAAAGCTTGAACTATTAATCGTGCCTGGGTGTACATCACTTACGAACCTTTCCAGTAATACTTGTTCACCAGCCCTCAGTTTTTTGAATGCTATGAATTGCATCAATCTTAAAGAGTTCTCAATCCCATTTTCTTCCGTTGATTTGAGTTTGTATTTTACAGCGTGGGATGGGAATGAACTTCCATCATCTATATTGCATGCACAAAATCTTAATGGCTTTGGCTTTGCTCTTAGTGATTGTCTTGTGAATCTTCCTGAAAACTTTTGTGATAATATTGGGCTTCATAGTCAACAGAATCGTGAACAGGACCCTTTCATCACCTTAGATAAAGTACTTTCTAGTCCTGCCTTCCTCAGTGTAAAATATTTGGACTTTTATAATATTCATATGTTGTCTGAAATCCCAGACAGTATCACCTTACTATCATCATTAGAGTTTTTAAGACTGGTTCAAATGTTCATTAAAAGCTTGCCTGAAAGCATTAAGTATCTTCCCCGACTCGGAGAGGTTCATGTTTACCAATGTACATTGCTTCAATCTATTCCGGCACTTCATCGCTTTATTCCAAATTTGGTTGTCTGGGATTGTGAATATCTTGAGAAAGTGTTGAGTTCAACAGTTGAACCATATGACAAATCTATTCCTGGTTATACTGCGCTCCTTAACTGCAAAGAATTGGATCCACATTCATACCAAACAGTTTTGAAAGATTCTATTGATGGGATTGAACATGAAGCAAGACGAAATTCAGAAATTGAATTTTTATATGATGACAATAATATTATTTGGAACTTCCTACCTGCTATGCCTGGCATGGAAAACTGGTTCCATTACTCTTCTACACAACTTTCTGTCACTCTTAAGCTTCCTTTTAACTTGTTGGGTTTTGCCTTTTACTTGGTTCTTTCTCAAGGCCATATGGGATATGATGTAGGTTTTGGATGTCAGTGCTACTTGGACAATAGTTCAGCTGAAAGTATCTTTATAACAAGTTTCAAAAGACCCAACTTCATAAACTATCCCTGGACCGATACTTCAATCAACATGATGTCAGATCATTTGCTTTTATGGTATGATCCAAGAAGTTGCAAGCAGATAATGGATGCAGTTGAACTAACAAAAGCCAATAGTGATGTGAATAGCACCAGTTACAATCCAAAGTTTACATTTACATTCTTTATTGATGAAAATCTAGATGATGAAGTAGCCATAAAAGAGTGTGGGTTTCGCTGGATATATCAAGAAGAAACAGTTCCTTCAACAATTTTTGAATCCCATGATGAAGAAGAGATTGTGCCTTCATCAGATCGTCAATCCAATGAGCAAGAAGAAATAGTTTCTAAAACAAATTTTGAATCAGATGATCTAGAATTAGACGAAACACGTGATATGAGGTGTTCACTTGAAGAGCTCCTGCATATTGGATTTCGTGTAGACCACATGAACAACACTTGGTTTGGTTCTACAAAAGAAGAAAGCAACAGCTGA

Protein sequence

>Msa1311550

MDMASSSSSSSNDATLKKYDVFISFRGEDTRVGFTSHLYAALCRSSLHTYIDNRIEKGNEVWVELVKAIKESTVFIVVFSENYASSTWCLNELVQIMECHKNEQIVVIPVFYHVDPSHVRKQTGSYGTALAKHKKQGNDREIQNWKNALFQVANLSGFHLTTYRTESNLIEDITRAVIRKLNQQSTIDLTCNFIPDENFWSIQSLIKFDSTEVQIIGLWGMGGIGKTTLATAMFQRVSFKYDGSCFFEKVTEVSKRNGINYTCNKLLSKLLKEDLDIDTPKLIPSMIIRRLKSMKSFIVLDDVHNSELLQNLIGVGHGWLGSGSTVIVTTRDKHVLMRGGIEKIYEVTKMNSRNSLRLFSFNAFNKVSPKDGYVELSKRAIDYARGNPLALKVLGSLLRCKSEIEWDCALAKLKKMPNNEIDSILRMSFNELEDTEKNIFLDIACFFKGSKREWITKILNECGFFADIGISNLLDKALVRVDSENCIQMHCLIQEMGKQIVREESPKNPKQRSRLCDPKEVYDVLKNNRGSEKVEAIFLDATEYTHVTLRPDAFEKMKNLRLLAVQDHKGVKSISFPHGLGLLPENLRCILWDGYPLKTLPLTSCLEMLVELSLKRSHVEKLWNGVVNLPNLEIIDLSWSKKLIECPNVSGSPNLKEVILRDCESMLEVDSSIFLLQKLELLIVPGCTSLTNLSSNTCSPALSFLNAMNCINLKEFSIPFSSVDLSLYFTAWDGNELPSSILHAQNLNGFGFALSDCLVNLPENFCDNIGLHSQQNREQDPFITLDKVLSSPAFLSVKYLDFYNIHMLSEIPDSITLLSSLEFLRLVQMFIKSLPESIKYLPRLGEVHVYQCTLLQSIPALHRFIPNLVVWDCEYLEKVLSSTVEPYDKSIPGYTALLNCKELDPHSYQTVLKDSIDGIEHEARRNSEIEFLYDDNNIIWNFLPAMPGMENWFHYSSTQLSVTLKLPFNLLGFAFYLVLSQGHMGYDVGFGCQCYLDNSSAESIFITSFKRPNFINYPWTDTSINMMSDHLLLWYDPRSCKQIMDAVELTKANSDVNSTSYNPKFTFTFFIDENLDDEVAIKECGFRWIYQEETVPSTIFESHDEEEIVPSSDRQSNEQEEIVSKTNFESDDLELDETRDMRCSLEELLHIGFRVDHMNNTWFGSTKEESNS*