AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1050490


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1050490 MtrunA17_Chr7g0219651 89.899 99 10 0 3 101 1770 1868 5.51e-57 188
Msa1050490 MtrunA17_Chr7g0219651 78.641 103 16 1 3 99 774 876 9.14e-48 162
Msa1050490 MtrunA17_Chr7g0219571 88.889 99 11 0 3 101 785 883 1.30e-56 187
Msa1050490 MtrunA17_Chr7g0219581 67.593 108 26 1 3 101 209 316 2.20e-44 145
Msa1050490 MtrunA17_Chr4g0025961 68.519 108 25 1 3 101 778 885 1.25e-42 147
Msa1050490 MtrunA17_Chr7g0219601 67.593 108 26 1 3 101 769 876 1.18e-39 139
Msa1050490 MtrunA17_Chr7g0219611 66.667 99 28 1 3 101 765 858 3.97e-34 123
Msa1050490 MtrunA17_Chr7g0219621 93.023 43 3 0 3 45 176 218 3.57e-21 83.6
Msa1050490 MtrunA17_Chr7g0219671 48.624 109 33 5 3 90 783 889 5.71e-20 83.2
Msa1050490 MtrunA17_Chr7g0219721 44.340 106 41 3 3 90 783 888 5.48e-17 74.7
Msa1050490 MtrunA17_Chr7g0219781 47.706 109 34 5 3 90 786 892 1.74e-16 73.2
Msa1050490 MtrunA17_Chr7g0219691 43.396 106 42 3 3 90 783 888 8.11e-16 71.2
Msa1050490 MtrunA17_Chr7g0219761 60.465 43 17 0 3 45 38 80 3.05e-11 55.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 23 sgRNAs with CRISPR-Local

Find 28 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAACAACCTTTCTTTGAGAT+TGG 0.303108 7_2:-15246403 Msa1050490:CDS
AACAACCTTTCTTTGAGATT+GGG 0.389275 7_2:-15246402 Msa1050490:CDS
AAAGGTTGTTCTTGAGTACT+TGG 0.402756 7_2:+15246415 None:intergenic
AGTGAGGAAGATAGTCAGTT+TGG 0.417130 7_2:-15246535 Msa1050490:CDS
CTTCAATGAGTCAAGTGGTT+GGG 0.422708 7_2:-15246588 Msa1050490:CDS
TTCAATGAGTCAAGTGGTTG+GGG 0.429791 7_2:-15246587 Msa1050490:CDS
CGAGACGGTACTCTCCTTGT+AGG 0.441050 7_2:+15246444 None:intergenic
TTAAGTTGTTTGCATGATGA+TGG 0.466658 7_2:-15246622 Msa1050490:CDS
TTTAGAGTTTGCGCTGCAAT+CGG 0.467593 7_2:-15246563 Msa1050490:CDS
TGATTCCCAATCTCAAAGAA+AGG 0.479223 7_2:+15246397 None:intergenic
CCAACCACTTGACTCATTGA+AGG 0.486871 7_2:+15246589 None:intergenic
GGACATATATGAGTGATGAA+TGG 0.511823 7_2:-15246495 Msa1050490:CDS
CACGAGATCGCACACCTACA+AGG 0.527211 7_2:-15246458 Msa1050490:CDS
TAAGTTGTTTGCATGATGAT+GGG 0.556475 7_2:-15246621 Msa1050490:CDS
AGTACTTGGCCTCGCCGAGA+CGG 0.577496 7_2:+15246429 None:intergenic
AGAGTTTGCGCTGCAATCGG+TGG 0.584786 7_2:-15246560 Msa1050490:CDS
CCTTCAATGAGTCAAGTGGT+TGG 0.599636 7_2:-15246589 Msa1050490:CDS
ACGACCTTCAATGAGTCAAG+TGG 0.623156 7_2:-15246593 Msa1050490:CDS
CTACAAGGAGAGTACCGTCT+CGG 0.656380 7_2:-15246443 Msa1050490:CDS
GCTGCAATCGGTGGTGAGTG+AGG 0.659967 7_2:-15246551 Msa1050490:CDS
TCAATGAGTCAAGTGGTTGG+GGG 0.661801 7_2:-15246586 Msa1050490:CDS
AGATTGGGAATCAAAGAACA+CGG 0.692151 7_2:-15246387 Msa1050490:CDS
AGGAGAGTACCGTCTCGGCG+AGG 0.710756 7_2:-15246438 Msa1050490:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ACATATAAGTTTTGAAGTTT+TGG + chr7_2:15246655-15246674 None:intergenic 20.0%
!! TTTGGAATATTATTGCAAGT+TGG - chr7_2:15246358-15246377 Msa1050490:CDS 25.0%
! TTAAGTTGTTTGCATGATGA+TGG - chr7_2:15246383-15246402 Msa1050490:CDS 30.0%
TAAGTTGTTTGCATGATGAT+GGG - chr7_2:15246384-15246403 Msa1050490:CDS 30.0%
! CATATATGTCCTCTCTTTTT+GGG + chr7_2:15246501-15246520 None:intergenic 30.0%
! TCATATATGTCCTCTCTTTT+TGG + chr7_2:15246502-15246521 None:intergenic 30.0%
!! AACAACCTTTCTTTGAGATT+GGG - chr7_2:15246603-15246622 Msa1050490:CDS 30.0%
GGACATATATGAGTGATGAA+TGG - chr7_2:15246510-15246529 Msa1050490:CDS 35.0%
AAAGGTTGTTCTTGAGTACT+TGG + chr7_2:15246593-15246612 None:intergenic 35.0%
!! GAACAACCTTTCTTTGAGAT+TGG - chr7_2:15246602-15246621 Msa1050490:CDS 35.0%
TGATTCCCAATCTCAAAGAA+AGG + chr7_2:15246611-15246630 None:intergenic 35.0%
AGATTGGGAATCAAAGAACA+CGG - chr7_2:15246618-15246637 Msa1050490:CDS 35.0%
CTTCAATGAGTCAAGTGGTT+GGG - chr7_2:15246417-15246436 Msa1050490:CDS 40.0%
TTCAATGAGTCAAGTGGTTG+GGG - chr7_2:15246418-15246437 Msa1050490:CDS 40.0%
! TTTAGAGTTTGCGCTGCAAT+CGG - chr7_2:15246442-15246461 Msa1050490:CDS 40.0%
AGTGAGGAAGATAGTCAGTT+TGG - chr7_2:15246470-15246489 Msa1050490:CDS 40.0%
ACGACCTTCAATGAGTCAAG+TGG - chr7_2:15246412-15246431 Msa1050490:CDS 45.0%
CCAACCACTTGACTCATTGA+AGG + chr7_2:15246419-15246438 None:intergenic 45.0%
CCTTCAATGAGTCAAGTGGT+TGG - chr7_2:15246416-15246435 Msa1050490:CDS 45.0%
TCAATGAGTCAAGTGGTTGG+GGG - chr7_2:15246419-15246438 Msa1050490:CDS 45.0%
TTGGTACGACCCAAAAAGAG+AGG - chr7_2:15246489-15246508 Msa1050490:CDS 45.0%
!! CTACAAGGAGAGTACCGTCT+CGG - chr7_2:15246562-15246581 Msa1050490:CDS 50.0%
AGAGTTTGCGCTGCAATCGG+TGG - chr7_2:15246445-15246464 Msa1050490:CDS 55.0%
CACGAGATCGCACACCTACA+AGG - chr7_2:15246547-15246566 Msa1050490:CDS 55.0%
CGAGACGGTACTCTCCTTGT+AGG + chr7_2:15246564-15246583 None:intergenic 55.0%
GCTGCAATCGGTGGTGAGTG+AGG - chr7_2:15246454-15246473 Msa1050490:CDS 60.0%
AGTACTTGGCCTCGCCGAGA+CGG + chr7_2:15246579-15246598 None:intergenic 60.0%
!! AGGAGAGTACCGTCTCGGCG+AGG - chr7_2:15246567-15246586 Msa1050490:CDS 65.0%
Chromosome Type Strat End Strand Name
chr7_2 gene 15246351 15246676 15246351 ID=Msa1050490;Name=Msa1050490
chr7_2 mRNA 15246351 15246676 15246351 ID=Msa1050490-mRNA-1;Parent=Msa1050490;Name=Msa1050490-mRNA-1;_AED=0.73;_eAED=0.74;_QI=0|0|0|1|0|0|2|0|101
chr7_2 exon 15246671 15246676 15246671 ID=Msa1050490-mRNA-1:exon:3644;Parent=Msa1050490-mRNA-1
chr7_2 exon 15246351 15246650 15246351 ID=Msa1050490-mRNA-1:exon:3643;Parent=Msa1050490-mRNA-1
chr7_2 CDS 15246671 15246676 15246671 ID=Msa1050490-mRNA-1:cds;Parent=Msa1050490-mRNA-1
chr7_2 CDS 15246351 15246650 15246351 ID=Msa1050490-mRNA-1:cds;Parent=Msa1050490-mRNA-1
Gene Sequence

>Msa1050490

ATGAGTTTGGCGTTAAGTTGTTTGCATGATGATGGGACTCAACGACCTTCAATGAGTCAAGTGGTTGGGGGTTTAGAGTTTGCGCTGCAATCGGTGGTGAGTGAGGAAGATAGTCAGTTTGGTACGACCCAAAAAGAGAGGACATATATGAGTGATGAATGGAGTGACTTGCATTTCACGAGATCGCACACCTACAAGGAGAGTACCGTCTCGGCGAGGCCAAGTACTCAAGAACAACCTTTCTTTGAGATTGGGAATCAAAGAACACGGTCGTATTCATGCCAAAACTTCAAAACTTATATGTAG

Protein sequence

>Msa1050490

MSLALSCLHDDGTQRPSMSQVVGGLEFALQSVVSEEDSQFGTTQKERTYMSDEWSDLHFTRSHTYKESTVSARPSTQEQPFFEIGNQRTRSYSCQNFKTYM*