AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0166620

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0166620 MtrunA17_Chr1g0181511 98.154 325 6 0 1 325 1 325 0.0 671
Msa0166620 MtrunA17_Chr1g0181481 88.000 325 39 0 1 325 1 325 0.0 564
Msa0166620 MtrunA17_Chr1g0181431 88.119 303 36 0 23 325 23 325 0.0 562
Msa0166620 MtrunA17_Chr4g0074851 58.769 325 125 2 8 323 4 328 4.74e-139 397
Msa0166620 MtrunA17_Chr1g0181501 80.000 190 37 1 136 325 3 191 1.20e-109 317
Msa0166620 MtrunA17_Chr5g0404721 43.000 300 161 4 31 323 34 330 2.19e-77 239
Msa0166620 MtrunA17_Chr8g0372601 39.809 314 178 5 17 323 21 330 6.10e-72 226
Msa0166620 MtrunA17_Chr4g0075241 36.452 310 191 4 18 323 20 327 2.39e-71 224
Msa0166620 MtrunA17_Chr7g0254201 38.033 305 179 4 22 323 15 312 2.48e-69 218
Msa0166620 MtrunA17_Chr3g0106921 36.697 327 196 6 1 323 1 320 1.51e-67 214
Msa0166620 MtrunA17_Chr5g0400331 37.736 318 183 4 18 323 17 331 8.66e-67 213
Msa0166620 MtrunA17_Chr7g0225221 39.117 317 185 5 12 323 7 320 1.03e-66 212
Msa0166620 MtrunA17_Chr6g0460711 34.591 318 196 4 12 323 3 314 8.53e-66 209
Msa0166620 MtrunA17_Chr2g0320031 35.962 317 196 4 9 323 8 319 1.42e-65 209
Msa0166620 MtrunA17_Chr7g0254231 37.919 298 178 3 27 324 22 312 1.65e-65 209
Msa0166620 MtrunA17_Chr3g0128061 36.025 322 196 4 11 324 8 327 2.43e-65 209
Msa0166620 MtrunA17_Chr5g0438021 34.862 327 200 4 7 323 2 325 4.68e-65 208
Msa0166620 MtrunA17_Chr3g0129051 34.579 321 192 6 12 323 11 322 1.21e-63 204
Msa0166620 MtrunA17_Chr4g0046131 36.184 304 182 5 26 319 41 342 1.46e-63 204
Msa0166620 MtrunA17_Chr1g0175561 35.404 322 194 5 12 324 11 327 4.02e-63 203
Msa0166620 MtrunA17_Chr1g0197411 35.185 324 199 5 9 323 9 330 5.65e-63 202
Msa0166620 MtrunA17_Chr2g0320101 34.268 321 202 4 7 323 2 317 1.08e-62 201
Msa0166620 MtrunA17_Chr2g0320071 34.579 321 201 4 7 323 2 317 1.13e-62 201
Msa0166620 MtrunA17_Chr2g0292211 36.923 325 192 5 9 324 22 342 2.35e-62 202
Msa0166620 MtrunA17_Chr1g0204011 35.562 329 199 6 1 323 1 322 2.86e-62 201
Msa0166620 MtrunA17_Chr2g0296621 38.816 304 169 6 27 324 50 342 4.31e-62 201
Msa0166620 MtrunA17_Chr2g0292251 36.422 313 188 4 21 325 22 331 6.73e-62 201
Msa0166620 MtrunA17_Chr4g0070951 35.061 328 194 8 10 324 9 330 7.27e-62 200
Msa0166620 MtrunA17_Chr6g0451571 34.796 319 200 4 11 323 18 334 9.44e-62 199
Msa0166620 MtrunA17_Chr7g0258811 35.692 325 197 6 9 324 51 372 9.67e-62 201
Msa0166620 MtrunA17_Chr2g0316191 34.545 330 181 5 25 319 74 403 2.33e-61 201
Msa0166620 MtrunA17_Chr2g0320051 35.082 305 183 5 25 323 23 318 3.09e-61 197
Msa0166620 MtrunA17_Chr6g0467121 35.093 322 189 6 14 324 52 364 3.48e-61 199
Msa0166620 MtrunA17_Chr2g0292241 37.963 324 187 6 10 324 13 331 3.51e-61 199
Msa0166620 MtrunA17_Chr2g0320061 34.579 321 192 6 12 323 7 318 6.15e-61 197
Msa0166620 MtrunA17_Chr7g0244461 31.935 310 205 3 15 323 8 312 8.98e-61 196
Msa0166620 MtrunA17_Chr1g0181491 80.531 113 21 1 24 136 9 120 3.96e-60 189
Msa0166620 MtrunA17_Chr2g0316481 34.650 329 181 6 25 319 74 402 8.82e-60 197
Msa0166620 MtrunA17_Chr8g0364291 37.855 317 188 3 14 323 10 324 9.26e-60 194
Msa0166620 MtrunA17_Chr5g0432441 36.164 318 196 5 9 323 13 326 1.35e-59 194
Msa0166620 MtrunA17_Chr2g0316831 34.650 329 181 5 25 319 110 438 1.39e-59 197
Msa0166620 MtrunA17_Chr2g0316791 34.650 329 181 5 25 319 74 402 1.69e-59 196
Msa0166620 MtrunA17_Chr4g0024121 34.347 329 207 3 1 323 1 326 2.10e-59 193
Msa0166620 MtrunA17_Chr3g0114451 35.099 302 190 3 23 323 22 318 2.73e-59 192
Msa0166620 MtrunA17_Chr5g0405991 36.478 318 189 6 11 323 7 316 5.30e-59 192
Msa0166620 MtrunA17_Chr4g0076211 37.542 301 172 6 29 323 43 333 7.93e-59 192
Msa0166620 MtrunA17_Chr1g0213061 33.750 320 199 7 12 324 9 322 1.17e-58 191
Msa0166620 MtrunA17_Chr2g0292181 37.461 323 190 6 10 324 22 340 1.51e-58 192
Msa0166620 MtrunA17_Chr2g0291751 37.459 307 181 4 25 323 27 330 2.63e-58 191
Msa0166620 MtrunA17_Chr2g0320091 32.177 317 206 5 11 323 7 318 5.16e-58 189
Msa0166620 MtrunA17_Chr4g0013201 34.333 300 191 3 25 323 44 338 1.16e-57 189
Msa0166620 MtrunA17_Chr6g0467161 33.438 320 196 6 14 323 24 336 1.24e-57 189
Msa0166620 MtrunA17_Chr4g0038151 32.166 314 211 2 11 323 9 321 1.64e-57 188
Msa0166620 MtrunA17_Chr3g0129061 34.185 313 200 4 13 323 14 322 1.65e-57 188
Msa0166620 MtrunA17_Chr2g0292151 34.727 311 192 4 21 323 23 330 3.87e-57 188
Msa0166620 MtrunA17_Chr4g0038171 33.229 319 201 5 11 323 9 321 1.15e-56 186
Msa0166620 MtrunA17_Chr8g0371381 35.974 303 181 5 26 323 25 319 1.85e-56 186
Msa0166620 MtrunA17_Chr1g0194151 34.516 310 188 5 25 323 28 333 3.06e-56 185
Msa0166620 MtrunA17_Chr7g0268501 34.891 321 195 5 11 323 6 320 5.53e-56 184
Msa0166620 MtrunA17_Chr7g0268521 34.891 321 195 5 11 323 6 320 5.53e-56 184
Msa0166620 MtrunA17_Chr2g0322861 34.848 330 203 6 1 323 62 386 5.59e-56 186
Msa0166620 MtrunA17_Chr4g0043391 32.915 319 202 5 11 323 12 324 1.50e-55 183
Msa0166620 MtrunA17_Chr3g0129041 34.505 313 199 4 13 323 14 322 1.60e-55 183
Msa0166620 MtrunA17_Chr6g0477071 33.003 303 193 4 22 323 49 342 2.10e-55 183
Msa0166620 MtrunA17_Chr2g0311251 33.434 332 186 6 25 322 9 339 2.29e-55 183
Msa0166620 MtrunA17_Chr1g0164891 34.098 305 190 5 28 323 90 392 3.50e-55 184
Msa0166620 MtrunA17_Chr5g0413051 33.766 308 196 5 24 323 70 377 4.80e-55 183
Msa0166620 MtrunA17_Chr2g0292111 34.405 311 193 4 21 323 23 330 4.91e-55 183
Msa0166620 MtrunA17_Chr4g0013821 33.225 307 191 6 25 324 26 325 8.93e-55 182
Msa0166620 MtrunA17_Chr2g0299691 35.526 304 182 7 31 324 60 359 9.26e-55 182
Msa0166620 MtrunA17_Chr2g0279041 32.416 327 202 6 9 323 6 325 1.24e-54 181
Msa0166620 MtrunA17_Chr4g0013721 33.770 305 190 5 25 323 98 396 1.83e-54 183
Msa0166620 MtrunA17_Chr2g0292261 34.568 324 200 6 10 325 13 332 2.51e-54 181
Msa0166620 MtrunA17_Chr7g0244451 32.692 312 204 3 13 323 10 316 3.45e-54 179
Msa0166620 MtrunA17_Chr4g0038161 32.166 314 210 3 11 323 9 320 3.60e-54 179
Msa0166620 MtrunA17_Chr5g0401431 35.514 321 195 5 11 322 11 328 7.35e-54 179
Msa0166620 MtrunA17_Chr6g0467251 33.108 296 188 5 27 319 36 324 1.16e-53 179
Msa0166620 MtrunA17_Chr2g0292101 33.746 323 199 6 12 325 12 328 1.33e-53 179
Msa0166620 MtrunA17_Chr4g0013191 34.653 303 189 5 25 323 43 340 1.98e-53 178
Msa0166620 MtrunA17_Chr2g0292091 32.704 318 201 5 16 325 15 327 2.11e-53 178
Msa0166620 MtrunA17_Chr4g0013171 35.625 320 196 6 9 323 7 321 2.67e-53 177
Msa0166620 MtrunA17_Chr1g0155611 37.705 305 179 4 27 323 46 347 3.76e-53 177
Msa0166620 MtrunA17_Chr4g0072091 34.551 301 186 5 26 319 23 319 1.52e-52 176
Msa0166620 MtrunA17_Chr5g0432351 35.077 325 195 8 11 325 5 323 1.82e-52 175
Msa0166620 MtrunA17_Chr1g0194271 33.438 317 189 5 25 323 28 340 2.19e-52 176
Msa0166620 MtrunA17_Chr3g0107881 31.988 322 205 7 11 323 8 324 4.31e-52 174
Msa0166620 MtrunA17_Chr3g0106901 33.841 328 203 4 1 323 5 323 6.78e-52 174
Msa0166620 MtrunA17_Chr4g0063671 32.000 325 196 6 13 323 7 320 8.10e-52 173
Msa0166620 MtrunA17_Chr1g0148021 33.837 331 191 7 13 323 6 328 8.68e-52 174
Msa0166620 MtrunA17_Chr2g0329641 32.248 307 197 4 24 323 33 335 8.71e-52 174
Msa0166620 MtrunA17_Chr2g0292271 32.308 325 209 4 9 325 11 332 8.92e-52 174
Msa0166620 MtrunA17_Chr4g0068621 32.895 304 196 4 25 323 36 336 1.39e-51 173
Msa0166620 MtrunA17_Chr4g0053871 34.615 312 197 4 12 319 12 320 2.80e-51 172
Msa0166620 MtrunA17_Chr2g0311241 29.491 373 187 6 25 322 58 429 5.45e-51 174
Msa0166620 MtrunA17_Chr1g0157051 31.962 316 203 4 12 322 9 317 5.54e-51 171
Msa0166620 MtrunA17_Chr5g0402571 33.022 321 205 6 9 323 10 326 5.93e-51 171
Msa0166620 MtrunA17_Chr1g0188431 31.987 297 194 3 27 323 39 327 2.46e-50 170
Msa0166620 MtrunA17_Chr7g0224371 31.132 318 207 5 13 324 12 323 3.78e-50 169
Msa0166620 MtrunA17_Chr4g0013181 35.690 297 183 5 30 323 62 353 1.45e-49 169
Msa0166620 MtrunA17_Chr5g0432331 34.314 306 187 7 25 323 54 352 1.53e-49 169
Msa0166620 MtrunA17_Chr5g0432341 34.426 305 188 7 25 323 23 321 3.50e-49 167
Msa0166620 MtrunA17_Chr5g0402581 31.988 322 207 6 9 323 10 326 4.52e-49 166
Msa0166620 MtrunA17_Chr3g0136751 32.087 321 204 5 11 323 8 322 5.21e-49 166
Msa0166620 MtrunA17_Chr2g0292291 32.595 316 193 6 21 323 24 332 5.28e-48 164
Msa0166620 MtrunA17_Chr5g0421411 32.130 277 176 5 58 324 12 286 2.11e-44 153
Msa0166620 MtrunA17_Chr2g0278671 32.063 315 187 7 24 323 21 323 4.20e-44 153
Msa0166620 MtrunA17_Chr2g0279061 32.319 263 162 5 70 323 11 266 2.48e-43 150
Msa0166620 MtrunA17_Chr2g0316811 36.842 228 118 4 25 228 48 273 1.54e-40 144
Msa0166620 MtrunA17_Chr5g0432401 28.213 319 176 7 11 322 13 285 1.49e-32 122
Msa0166620 MtrunA17_Chr4g0004591 25.157 318 177 4 27 322 77 355 1.88e-30 118
Msa0166620 MtrunA17_Chr2g0316141 29.433 282 155 7 74 324 12 280 1.66e-29 114
Msa0166620 MtrunA17_Chr2g0316181 29.433 282 155 7 74 324 12 280 1.66e-29 114
Msa0166620 MtrunA17_Chr2g0292281 32.394 213 124 4 119 324 18 217 7.04e-28 108
Msa0166620 MtrunA17_Chr3g0114461 33.333 189 120 3 136 323 9 192 2.04e-25 100
Msa0166620 MtrunA17_Chr3g0114491 40.179 112 66 1 23 133 14 125 9.65e-24 94.7
Msa0166620 MtrunA17_Chr7g0254181 36.232 138 84 2 89 225 22 156 7.93e-16 73.9
Msa0166620 MtrunA17_Chr6g0468731 43.662 71 37 1 66 136 22 89 9.84e-15 68.9
Msa0166620 MtrunA17_Chr7g0254171 28.244 131 94 0 70 200 1 131 8.37e-12 62.0
Msa0166620 MtrunA17_Chr3g0104101 30.769 143 88 4 191 324 10 150 9.69e-12 62.4
Msa0166620 MtrunA17_Chr5g0432321 29.508 122 80 2 147 268 17 132 4.48e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0166620 AT2G37130.1 75.082 305 75 1 20 323 23 327 1.07e-176 492
Msa0166620 AT2G37130.2 76.351 296 69 1 29 323 1 296 1.57e-173 483
Msa0166620 AT4G21960.1 57.525 299 127 0 27 325 31 329 3.42e-134 385
Msa0166620 AT4G36430.1 39.130 322 184 5 10 324 15 331 2.41e-72 227
Msa0166620 AT1G34510.1 38.217 314 180 6 11 323 9 309 9.42e-72 225
Msa0166620 AT5G66390.1 39.333 300 172 4 31 323 37 333 2.77e-70 222
Msa0166620 AT2G18150.1 39.414 307 176 4 24 323 33 336 4.72e-70 221
Msa0166620 AT3G03670.1 37.812 320 185 3 12 323 8 321 1.45e-69 219
Msa0166620 AT5G17820.1 37.342 316 181 5 13 323 10 313 2.78e-69 219
Msa0166620 AT3G50990.1 40.196 306 173 4 25 323 39 341 2.34e-68 217
Msa0166620 AT5G17820.2 37.658 316 180 5 13 323 63 366 5.22e-68 217
Msa0166620 AT2G18140.1 37.821 312 184 4 19 323 27 335 3.12e-67 214
Msa0166620 AT4G08780.1 35.783 313 188 5 22 324 19 328 5.51e-66 211
Msa0166620 AT5G40150.1 36.893 309 183 3 21 323 26 328 1.83e-65 209
Msa0166620 AT3G17070.1 35.061 328 196 7 10 323 15 339 3.89e-65 209
Msa0166620 AT4G26010.1 36.102 313 189 5 12 323 7 309 5.73e-65 207
Msa0166620 AT4G26010.2 36.392 316 183 6 9 323 39 337 1.26e-64 207
Msa0166620 AT4G08770.1 35.016 317 193 5 18 324 15 328 3.07e-64 207
Msa0166620 AT5G14130.1 36.050 319 194 3 11 323 16 330 5.28e-64 206
Msa0166620 AT5G05340.1 35.738 305 190 4 21 323 24 324 8.49e-64 205
Msa0166620 AT5G14130.2 36.050 319 194 3 11 323 57 371 2.73e-63 205
Msa0166620 AT5G15180.1 36.527 334 196 7 1 323 1 329 9.99e-63 202
Msa0166620 AT3G32980.1 34.375 320 197 5 16 325 20 336 7.45e-62 201
Msa0166620 AT1G05260.1 33.841 328 200 6 10 324 3 326 2.38e-61 199
Msa0166620 AT4G17690.1 36.789 299 185 2 27 323 26 322 2.60e-61 199
Msa0166620 AT5G06730.1 32.544 338 211 6 1 324 1 335 4.24e-61 199
Msa0166620 AT1G71695.1 35.548 301 185 4 27 323 44 339 8.23e-61 198
Msa0166620 AT1G30870.1 37.255 306 174 6 27 324 51 346 3.83e-60 196
Msa0166620 AT3G49120.1 33.750 320 199 5 16 325 21 337 6.91e-60 196
Msa0166620 AT2G22420.1 34.906 318 194 6 12 323 9 319 1.06e-59 194
Msa0166620 AT4G31760.1 34.639 332 202 5 1 323 1 326 1.24e-59 194
Msa0166620 AT5G06720.1 33.022 321 202 5 14 324 17 334 1.40e-59 194
Msa0166620 AT2G39040.1 37.217 309 184 6 24 323 43 350 1.54e-59 195
Msa0166620 AT1G14550.1 35.404 322 197 6 9 323 4 321 6.22e-59 192
Msa0166620 AT1G24110.1 35.714 322 199 3 8 323 3 322 7.78e-59 192
Msa0166620 AT5G64120.1 33.225 307 186 5 25 323 33 328 1.65e-58 191
Msa0166620 AT3G28200.1 34.853 307 187 4 23 323 17 316 3.02e-58 190
Msa0166620 AT2G35380.1 34.615 312 183 5 27 323 30 335 3.78e-58 191
Msa0166620 AT3G01190.1 36.254 331 193 9 1 323 1 321 4.53e-58 190
Msa0166620 AT2G18980.1 33.639 327 205 4 4 323 2 323 1.23e-57 189
Msa0166620 AT4G16270.1 36.184 304 182 6 27 323 50 348 2.59e-57 189
Msa0166620 AT2G34060.1 35.410 305 185 5 26 320 41 343 3.04e-57 189
Msa0166620 AT3G49110.1 32.812 320 202 5 16 325 22 338 4.96e-57 188
Msa0166620 AT4G33870.1 30.523 344 202 5 13 319 54 397 7.32e-57 189
Msa0166620 AT2G38390.1 33.123 317 200 5 16 323 20 333 1.00e-56 187
Msa0166620 AT2G24800.1 33.660 306 191 4 25 323 29 329 8.47e-56 184
Msa0166620 AT5G47000.1 35.000 300 190 3 27 323 33 330 1.78e-55 184
Msa0166620 AT2G38380.1 33.123 317 200 5 16 323 20 333 2.96e-55 184
Msa0166620 AT1G14540.1 33.544 316 203 5 11 323 4 315 3.40e-55 182
Msa0166620 AT2G41480.1 34.347 329 194 10 9 323 8 328 4.87e-55 182
Msa0166620 AT1G44970.1 37.459 307 181 4 25 323 43 346 8.69e-55 182
Msa0166620 AT5G58390.1 33.121 314 205 4 12 323 6 316 1.18e-54 181
Msa0166620 AT1G49570.1 32.013 303 200 2 25 321 46 348 1.81e-54 181
Msa0166620 AT5G42180.1 33.438 317 197 5 12 322 8 316 3.43e-54 180
Msa0166620 AT3G21770.1 35.644 303 184 7 24 319 26 324 4.29e-54 180
Msa0166620 AT5G24070.1 33.657 309 191 5 26 325 34 337 4.52e-54 180
Msa0166620 AT4G11290.1 32.615 325 200 7 12 323 8 326 4.86e-54 180
Msa0166620 AT4G30170.1 33.660 306 191 5 25 323 25 325 8.60e-54 179
Msa0166620 AT1G68850.1 33.117 308 195 6 27 324 30 336 9.78e-54 179
Msa0166620 AT4G33420.1 33.439 314 197 4 14 323 20 325 1.41e-53 179
Msa0166620 AT5G58400.1 32.390 318 203 6 13 323 13 325 1.71e-53 178
Msa0166620 AT4G37520.1 33.766 308 192 5 25 323 25 329 2.06e-53 178
Msa0166620 AT5G51890.1 33.022 321 202 6 9 323 9 322 3.99e-53 177
Msa0166620 AT4G33420.2 33.439 314 197 4 14 323 67 372 6.00e-53 178
Msa0166620 AT5G39580.1 32.704 318 200 7 11 323 11 319 2.66e-52 175
Msa0166620 AT1G05240.1 33.660 306 188 5 27 323 26 325 4.15e-52 175
Msa0166620 AT1G05250.1 33.660 306 188 5 27 323 26 325 4.15e-52 175
Msa0166620 AT4G25980.1 32.602 319 199 7 15 323 14 326 8.26e-52 174
Msa0166620 AT4G37530.1 33.441 311 195 5 22 323 22 329 9.42e-52 174
Msa0166620 AT4G37520.2 33.766 308 189 6 25 323 25 326 1.65e-51 173
Msa0166620 AT5G22410.1 32.622 328 207 6 1 320 1 322 2.02e-50 171
Msa0166620 AT5G19880.1 32.099 324 205 6 12 323 9 329 2.21e-50 170
Msa0166620 AT5G19890.1 33.117 308 191 6 24 323 27 327 2.37e-50 170
Msa0166620 AT3G17070.2 36.047 258 154 6 74 323 22 276 3.88e-49 166
Msa0166620 AT3G49960.1 32.792 308 195 5 25 323 25 329 7.65e-49 166
Msa0166620 AT1G77100.1 33.956 321 196 11 12 323 6 319 1.61e-48 165
Msa0166620 AT5G67400.1 33.766 308 192 5 25 323 25 329 2.19e-48 165
Msa0166620 AT2G43480.1 31.922 307 195 5 26 323 34 335 4.76e-48 164
Msa0166620 AT5G64110.1 31.579 304 192 6 28 323 35 330 1.26e-43 153
Msa0166620 AT4G37530.2 33.712 264 163 5 22 276 22 282 2.52e-43 150
Msa0166620 AT2G35380.2 32.129 249 149 4 89 323 5 247 8.00e-41 143
Msa0166620 AT5G64100.1 28.852 305 197 6 28 323 38 331 2.36e-38 139
Msa0166620 AT4G25980.2 36.723 177 109 2 15 191 59 232 1.06e-34 128
Msa0166620 AT5G39580.2 32.461 191 123 3 11 201 11 195 9.50e-29 111
Msa0166620 AT2G24800.2 34.132 167 107 1 25 191 29 192 1.80e-28 110
Msa0166620 AT3G42570.1 35.714 98 58 2 97 191 52 147 1.50e-13 67.8

Find 79 sgRNAs with CRISPR-Local

Find 242 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAATTAGTTATATGATTATT+TGG 0.150626 1_4:-48026133 Msa0166620:exon
CCTGTCTTCATTTCAAATTT+TGG 0.167361 1_4:+48026796 None:intergenic
TTGATGATCTCTTCTGCTTT+TGG 0.188067 1_4:+48027314 None:intergenic
CAACTACTACAAGAACATTT+TGG 0.233199 1_4:-48026349 Msa0166620:CDS
TTCACATAAGGTGATGTTCT+TGG 0.257639 1_4:+48026282 None:intergenic
AAGGGTACATTGCATGAATT+TGG 0.274410 1_4:-48026517 Msa0166620:CDS
ACATTTAAACTTGCAAAATA+TGG 0.277691 1_4:-48027694 Msa0166620:exon
CAAATAATCATATAACTAAT+TGG 0.281961 1_4:+48026134 None:intergenic
TTTCTCAACAGCAGCTTTGA+TGG 0.284452 1_4:+48027020 None:intergenic
GCATATGTGGGGTCTAGCTT+TGG 0.306524 1_4:+48026468 None:intergenic
CAGACATCAGAGAGAAGTTC+CGG 0.336911 1_4:-48027069 Msa0166620:CDS
ACAGCATTTGGATCTGGATT+TGG 0.338871 1_4:+48026417 None:intergenic
CTTGCATATAGAACAGCATT+TGG 0.340382 1_4:+48026405 None:intergenic
CTAAGGATATGGAGTCATTA+TGG 0.354994 1_4:+48026719 None:intergenic
TTGGATCTGGATTTGGTGTT+GGG 0.357857 1_4:+48026424 None:intergenic
ACGAGAAAGCACTAAGGATA+TGG 0.361019 1_4:+48026708 None:intergenic
TCAGAGAATAATCCTCTTAC+CGG 0.371092 1_4:-48026198 Msa0166620:CDS
TCTAGCTTTGGGTCCACTGT+GGG 0.394817 1_4:+48026480 None:intergenic
CATATGTGGGGTCTAGCTTT+GGG 0.402844 1_4:+48026469 None:intergenic
GAAGCCACTGTTGCTCTGTT+AGG 0.406342 1_4:-48026661 Msa0166620:intron
TCACCTTGATCTCCGGTAAG+AGG 0.408149 1_4:+48026186 None:intergenic
CCACTGTGGGGTATAGCCTC+TGG 0.422051 1_4:+48026493 None:intergenic
GATATGGAGTCATTATGGTT+TGG 0.435385 1_4:+48026724 None:intergenic
TCTAATTTCACCTTGATCTC+CGG 0.439413 1_4:+48026179 None:intergenic
TATAGAACAGCATTTGGATC+TGG 0.452583 1_4:+48026411 None:intergenic
TTTGGATCTGGATTTGGTGT+TGG 0.455653 1_4:+48026423 None:intergenic
AGTAGTAGTTCATTTGTAGC+TGG 0.459722 1_4:+48027348 None:intergenic
ATGGAAACACGGCGGTTTCG+TGG 0.470766 1_4:-48027262 Msa0166620:CDS
AGACATCAGAGAGAAGTTCC+GGG 0.476089 1_4:-48027068 Msa0166620:CDS
TAGTTGTTGTCGATTATCAT+TGG 0.485138 1_4:+48026366 None:intergenic
AATCCTCTTACCGGAGATCA+AGG 0.499067 1_4:-48026189 Msa0166620:CDS
GTCGGTAGGCGATGTAGTAT+CGG 0.505099 1_4:-48027094 Msa0166620:CDS
AGAATAATTAATAATGCCAT+CGG 0.509700 1_4:-48026078 Msa0166620:three_prime_UTR
GTCTAGCTTTGGGTCCACTG+TGG 0.510662 1_4:+48026479 None:intergenic
TCACCTTGACTATGCAATCA+TGG 0.511245 1_4:+48027225 None:intergenic
TTTAGATAGGCAGCATATGT+GGG 0.512711 1_4:+48026456 None:intergenic
CGCCTACCGACTCTAAAAGC+AGG 0.513021 1_4:+48027106 None:intergenic
CCAAAATTTGAAATGAAGAC+AGG 0.514773 1_4:-48026796 Msa0166620:CDS
GTAGGAGAAGCAATAGAAAG+AGG 0.524679 1_4:+48027643 None:intergenic
ATTCTTCTACCATTGTTACA+TGG 0.531531 1_4:+48026752 None:intergenic
CGCTAGAGATGGAATTGCCA+TGG 0.534187 1_4:-48026953 Msa0166620:intron
GAAATTAGAACGAGGCTTGG+TGG 0.536223 1_4:+48027671 None:intergenic
GTTGCTCTTTCCGCTAGAGA+TGG 0.543064 1_4:-48026964 Msa0166620:CDS
GAATAATTAATAATGCCATC+GGG 0.544695 1_4:-48026077 Msa0166620:three_prime_UTR
AGATCAAGGTGAAATTAGAA+AGG 0.559276 1_4:-48026175 Msa0166620:CDS
CAGGAGGACACTCAGTGGGA+AGG 0.566579 1_4:-48026536 Msa0166620:CDS
ACTTCAATGAACAATTCTCA+AGG 0.567274 1_4:-48026236 Msa0166620:CDS
ATACTTGAAGTTTCGCATCC+CGG 0.570050 1_4:+48027050 None:intergenic
CTAGCTTTGGGTCCACTGTG+GGG 0.577225 1_4:+48026481 None:intergenic
AGGAGGACACTCAGTGGGAA+GGG 0.577554 1_4:-48026535 Msa0166620:CDS
TGGAAACACGGCGGTTTCGT+GGG 0.579986 1_4:-48027261 Msa0166620:CDS
ATTGAACTGTATAACGAGCA+TGG 0.584782 1_4:-48027281 Msa0166620:CDS
CAAAGAAAGCCATGTAACAA+TGG 0.587531 1_4:-48026761 Msa0166620:CDS
ACAGAAATTAGAACGAGGCT+TGG 0.588243 1_4:+48027668 None:intergenic
TTGAAATGAAGACAGGACGA+AGG 0.588626 1_4:-48026789 Msa0166620:CDS
ATGAAGACAGGACGAAGGGA+TGG 0.591567 1_4:-48026784 Msa0166620:CDS
TGAAATGAAGACAGGACGAA+GGG 0.593661 1_4:-48026788 Msa0166620:CDS
TTGAAAACGAGAAAGCACTA+AGG 0.594148 1_4:+48026702 None:intergenic
CTTCCATGATTGCATAGTCA+AGG 0.595237 1_4:-48027228 Msa0166620:intron
GTCGGCACATGATACTGTCA+AGG 0.601258 1_4:+48026990 None:intergenic
GTATAACGAGCATGGAAACA+CGG 0.609604 1_4:-48027273 Msa0166620:CDS
GGTGCATTTGTATAGTTGGG+AGG 0.613238 1_4:-48026820 Msa0166620:intron
GCCTACCGACTCTAAAAGCA+GGG 0.614896 1_4:+48027107 None:intergenic
CTTCAATGAACAATTCTCAA+GGG 0.615864 1_4:-48026235 Msa0166620:CDS
GTTCTTCATCCACAGTGAGT+AGG 0.617420 1_4:+48026316 None:intergenic
CAATCAGGAGGACACTCAGT+GGG 0.618173 1_4:-48026540 Msa0166620:intron
ATTGCATGAATTTGGTCCAG+AGG 0.619021 1_4:-48026509 Msa0166620:CDS
GCATAAAAGTCTAAGCCCGA+TGG 0.642853 1_4:+48026062 None:intergenic
AGCGGAAAGAGCAACAATGT+CGG 0.649811 1_4:+48026972 None:intergenic
GTTACTTGATCAACCGACAA+AGG 0.652288 1_4:-48026013 Msa0166620:three_prime_UTR
CATGGCAATTCCATCTCTAG+CGG 0.653048 1_4:+48026954 None:intergenic
TTAGATAGGCAGCATATGTG+GGG 0.658332 1_4:+48026457 None:intergenic
AGTACCTAACAGAGCAACAG+TGG 0.665213 1_4:+48026657 None:intergenic
TCAATCAGGAGGACACTCAG+TGG 0.675558 1_4:-48026541 Msa0166620:intron
TCGGCACATGATACTGTCAA+GGG 0.683470 1_4:+48026991 None:intergenic
ACACAAGGGCCTACTCACTG+TGG 0.693288 1_4:-48026325 Msa0166620:CDS
AGGAAACAGAAATTAGAACG+AGG 0.744890 1_4:+48027663 None:intergenic
CCAGAGGCTATACCCCACAG+TGG 0.772742 1_4:-48026493 Msa0166620:CDS
TAACGAGCATGGAAACACGG+CGG 0.832552 1_4:-48027270 Msa0166620:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAACAAAATTAAAATTAAAA+TGG + chr1_4:48026185-48026204 None:intergenic 10.0%
!! CAACAAAATTAAAATTAAAA+TGG + chr1_4:48026185-48026204 None:intergenic 10.0%
!!! GACCAATATTTATTTATTTT+TGG - chr1_4:48026050-48026069 Msa0166620:three_prime_UTR 15.0%
!! CACCAAAAATAAATAAATAT+TGG + chr1_4:48026055-48026074 None:intergenic 15.0%
!! TATATATAGGTCTTAAATAT+TGG + chr1_4:48026087-48026106 None:intergenic 15.0%
!! TAATGCATACTAATATATAT+AGG + chr1_4:48026100-48026119 None:intergenic 15.0%
!!! AATTGATAATGATATATATG+TGG - chr1_4:48026460-48026479 Msa0166620:CDS 15.0%
!!! ATTGATAATGATATATATGT+GGG - chr1_4:48026461-48026480 Msa0166620:CDS 15.0%
!! TTATAGAACATTGTAAATAT+TGG - chr1_4:48026755-48026774 Msa0166620:CDS 15.0%
!! TATAGAACATTGTAAATATT+GGG - chr1_4:48026756-48026775 Msa0166620:CDS 15.0%
!!! ATTCTAACATTTACTTTTTT+TGG - chr1_4:48026988-48027007 Msa0166620:CDS 15.0%
!! CAAACATAAACATAATTAAT+TGG - chr1_4:48027041-48027060 Msa0166620:CDS 15.0%
!! CAAATAATCATATAACTAAT+TGG + chr1_4:48027484-48027503 None:intergenic 15.0%
!! CAATTAGTTATATGATTATT+TGG - chr1_4:48027482-48027501 Msa0166620:intron 15.0%
!!! GACCAATATTTATTTATTTT+TGG - chr1_4:48026050-48026069 Msa0166620:three_prime_UTR 15.0%
!! CACCAAAAATAAATAAATAT+TGG + chr1_4:48026055-48026074 None:intergenic 15.0%
!! TATATATAGGTCTTAAATAT+TGG + chr1_4:48026087-48026106 None:intergenic 15.0%
!! TAATGCATACTAATATATAT+AGG + chr1_4:48026100-48026119 None:intergenic 15.0%
!!! AATTGATAATGATATATATG+TGG - chr1_4:48026460-48026479 Msa0166620:CDS 15.0%
!!! ATTGATAATGATATATATGT+GGG - chr1_4:48026461-48026480 Msa0166620:CDS 15.0%
!! TTATAGAACATTGTAAATAT+TGG - chr1_4:48026755-48026774 Msa0166620:CDS 15.0%
!! TATAGAACATTGTAAATATT+GGG - chr1_4:48026756-48026775 Msa0166620:CDS 15.0%
!!! ATTCTAACATTTACTTTTTT+TGG - chr1_4:48026988-48027007 Msa0166620:CDS 15.0%
!! CAAACATAAACATAATTAAT+TGG - chr1_4:48027041-48027060 Msa0166620:CDS 15.0%
!! CAAATAATCATATAACTAAT+TGG + chr1_4:48027484-48027503 None:intergenic 15.0%
!! CAATTAGTTATATGATTATT+TGG - chr1_4:48027482-48027501 Msa0166620:intron 15.0%
!! ACATTTAAACTTGCAAAATA+TGG - chr1_4:48025921-48025940 Msa0166620:three_prime_UTR 20.0%
!! AGAATAATTAATAATGCCAT+CGG - chr1_4:48027537-48027556 Msa0166620:intron 20.0%
!! ACATTTAAACTTGCAAAATA+TGG - chr1_4:48025921-48025940 Msa0166620:three_prime_UTR 20.0%
!! AGAATAATTAATAATGCCAT+CGG - chr1_4:48027537-48027556 Msa0166620:intron 20.0%
!!! CTTCTCCTACATTTTTATTT+GGG - chr1_4:48025985-48026004 Msa0166620:intron 25.0%
! ATGTACCCAAATAAAAATGT+AGG + chr1_4:48025993-48026012 None:intergenic 25.0%
! TTAATTAGAGAGGAGATTAA+TGG + chr1_4:48026216-48026235 None:intergenic 25.0%
! TTTCCTATGCAAAAAATACA+TGG + chr1_4:48026244-48026263 None:intergenic 25.0%
! GAATAATTAATAATGCCATC+GGG - chr1_4:48027538-48027557 Msa0166620:intron 25.0%
!! TTATTTTCTATTGCCTTTGT+CGG + chr1_4:48027618-48027637 None:intergenic 25.0%
!!! CTTCTCCTACATTTTTATTT+GGG - chr1_4:48025985-48026004 Msa0166620:intron 25.0%
! ATGTACCCAAATAAAAATGT+AGG + chr1_4:48025993-48026012 None:intergenic 25.0%
! TTAATTAGAGAGGAGATTAA+TGG + chr1_4:48026216-48026235 None:intergenic 25.0%
! TTTCCTATGCAAAAAATACA+TGG + chr1_4:48026244-48026263 None:intergenic 25.0%
! GAATAATTAATAATGCCATC+GGG - chr1_4:48027538-48027557 Msa0166620:intron 25.0%
!! TTATTTTCTATTGCCTTTGT+CGG + chr1_4:48027618-48027637 None:intergenic 25.0%
! GCTTCTCCTACATTTTTATT+TGG - chr1_4:48025984-48026003 Msa0166620:intron 30.0%
ATATTGGTCACACAAAAATG+AGG + chr1_4:48026039-48026058 None:intergenic 30.0%
CATGGCATTATTAATTAGAG+AGG + chr1_4:48026226-48026245 None:intergenic 30.0%
!!! ATGCCATGTATTTTTTGCAT+AGG - chr1_4:48026238-48026257 Msa0166620:CDS 30.0%
! TTGGGTGTGAATTTTTATGT+TGG - chr1_4:48026774-48026793 Msa0166620:CDS 30.0%
! CCTGTCTTCATTTCAAATTT+TGG + chr1_4:48026822-48026841 None:intergenic 30.0%
! CCAAAATTTGAAATGAAGAC+AGG - chr1_4:48026819-48026838 Msa0166620:intron 30.0%
ATTCTTCTACCATTGTTACA+TGG + chr1_4:48026866-48026885 None:intergenic 30.0%
! TAGTTGTTGTCGATTATCAT+TGG + chr1_4:48027252-48027271 None:intergenic 30.0%
! CAACTACTACAAGAACATTT+TGG - chr1_4:48027266-48027285 Msa0166620:CDS 30.0%
!!! AAGAACATTTTGGAACACAA+GGG - chr1_4:48027276-48027295 Msa0166620:CDS 30.0%
ATCACCTTATGTGAAAAAGA+TGG - chr1_4:48027341-48027360 Msa0166620:CDS 30.0%
ACTTCAATGAACAATTCTCA+AGG - chr1_4:48027379-48027398 Msa0166620:intron 30.0%
CTTCAATGAACAATTCTCAA+GGG - chr1_4:48027380-48027399 Msa0166620:intron 30.0%
AGATCAAGGTGAAATTAGAA+AGG - chr1_4:48027440-48027459 Msa0166620:intron 30.0%
AATACACTCACAAACTTCAA+AGG + chr1_4:48027645-48027664 None:intergenic 30.0%
! GCTTCTCCTACATTTTTATT+TGG - chr1_4:48025984-48026003 Msa0166620:intron 30.0%
ATATTGGTCACACAAAAATG+AGG + chr1_4:48026039-48026058 None:intergenic 30.0%
CATGGCATTATTAATTAGAG+AGG + chr1_4:48026226-48026245 None:intergenic 30.0%
!!! ATGCCATGTATTTTTTGCAT+AGG - chr1_4:48026238-48026257 Msa0166620:CDS 30.0%
! TTGGGTGTGAATTTTTATGT+TGG - chr1_4:48026774-48026793 Msa0166620:CDS 30.0%
! CCTGTCTTCATTTCAAATTT+TGG + chr1_4:48026822-48026841 None:intergenic 30.0%
! CCAAAATTTGAAATGAAGAC+AGG - chr1_4:48026819-48026838 Msa0166620:intron 30.0%
ATTCTTCTACCATTGTTACA+TGG + chr1_4:48026866-48026885 None:intergenic 30.0%
! TAGTTGTTGTCGATTATCAT+TGG + chr1_4:48027252-48027271 None:intergenic 30.0%
! CAACTACTACAAGAACATTT+TGG - chr1_4:48027266-48027285 Msa0166620:CDS 30.0%
!!! AAGAACATTTTGGAACACAA+GGG - chr1_4:48027276-48027295 Msa0166620:CDS 30.0%
ATCACCTTATGTGAAAAAGA+TGG - chr1_4:48027341-48027360 Msa0166620:CDS 30.0%
ACTTCAATGAACAATTCTCA+AGG - chr1_4:48027379-48027398 Msa0166620:intron 30.0%
CTTCAATGAACAATTCTCAA+GGG - chr1_4:48027380-48027399 Msa0166620:intron 30.0%
AGATCAAGGTGAAATTAGAA+AGG - chr1_4:48027440-48027459 Msa0166620:intron 30.0%
AATACACTCACAAACTTCAA+AGG + chr1_4:48027645-48027664 None:intergenic 30.0%
AGGAAACAGAAATTAGAACG+AGG + chr1_4:48025955-48025974 None:intergenic 35.0%
AGGTCTTAAATATTGGTCAC+AGG + chr1_4:48026080-48026099 None:intergenic 35.0%
AGTAGTAGTTCATTTGTAGC+TGG + chr1_4:48026270-48026289 None:intergenic 35.0%
!! TGATGATCTCTTCTGCTTTT+GGG + chr1_4:48026303-48026322 None:intergenic 35.0%
!! TTGATGATCTCTTCTGCTTT+TGG + chr1_4:48026304-48026323 None:intergenic 35.0%
ATTGAACTGTATAACGAGCA+TGG - chr1_4:48026334-48026353 Msa0166620:CDS 35.0%
TGTTGGTGCATTTGTATAGT+TGG - chr1_4:48026791-48026810 Msa0166620:CDS 35.0%
GTTGGTGCATTTGTATAGTT+GGG - chr1_4:48026792-48026811 Msa0166620:CDS 35.0%
CAAAGAAAGCCATGTAACAA+TGG - chr1_4:48026854-48026873 Msa0166620:intron 35.0%
GATATGGAGTCATTATGGTT+TGG + chr1_4:48026894-48026913 None:intergenic 35.0%
CTAAGGATATGGAGTCATTA+TGG + chr1_4:48026899-48026918 None:intergenic 35.0%
!! TTGAAAACGAGAAAGCACTA+AGG + chr1_4:48026916-48026935 None:intergenic 35.0%
!! CTTTCTCGTTTTCAAGCTAT+TGG - chr1_4:48026921-48026940 Msa0166620:intron 35.0%
! TTGGTCACTTTGATTCAATC+AGG - chr1_4:48027060-48027079 Msa0166620:CDS 35.0%
! AAGGGTACATTGCATGAATT+TGG - chr1_4:48027098-48027117 Msa0166620:CDS 35.0%
TTTAGATAGGCAGCATATGT+GGG + chr1_4:48027162-48027181 None:intergenic 35.0%
! TTTTAGATAGGCAGCATATG+TGG + chr1_4:48027163-48027182 None:intergenic 35.0%
!!! TGGGCATCTAAGTTTTAGAT+AGG + chr1_4:48027175-48027194 None:intergenic 35.0%
TATAGAACAGCATTTGGATC+TGG + chr1_4:48027207-48027226 None:intergenic 35.0%
CTTGCATATAGAACAGCATT+TGG + chr1_4:48027213-48027232 None:intergenic 35.0%
!!! CAAGAACATTTTGGAACACA+AGG - chr1_4:48027275-48027294 Msa0166620:CDS 35.0%
TTCACATAAGGTGATGTTCT+TGG + chr1_4:48027336-48027355 None:intergenic 35.0%
AAAAAGATGGCAGCTGATAA+TGG - chr1_4:48027354-48027373 Msa0166620:CDS 35.0%
TCAGAGAATAATCCTCTTAC+CGG - chr1_4:48027417-48027436 Msa0166620:intron 35.0%
TCTAATTTCACCTTGATCTC+CGG + chr1_4:48027439-48027458 None:intergenic 35.0%
AGGAAACAGAAATTAGAACG+AGG + chr1_4:48025955-48025974 None:intergenic 35.0%
AGGTCTTAAATATTGGTCAC+AGG + chr1_4:48026080-48026099 None:intergenic 35.0%
AGTAGTAGTTCATTTGTAGC+TGG + chr1_4:48026270-48026289 None:intergenic 35.0%
!! TGATGATCTCTTCTGCTTTT+GGG + chr1_4:48026303-48026322 None:intergenic 35.0%
!! TTGATGATCTCTTCTGCTTT+TGG + chr1_4:48026304-48026323 None:intergenic 35.0%
ATTGAACTGTATAACGAGCA+TGG - chr1_4:48026334-48026353 Msa0166620:CDS 35.0%
TGTTGGTGCATTTGTATAGT+TGG - chr1_4:48026791-48026810 Msa0166620:CDS 35.0%
GTTGGTGCATTTGTATAGTT+GGG - chr1_4:48026792-48026811 Msa0166620:CDS 35.0%
CAAAGAAAGCCATGTAACAA+TGG - chr1_4:48026854-48026873 Msa0166620:intron 35.0%
GATATGGAGTCATTATGGTT+TGG + chr1_4:48026894-48026913 None:intergenic 35.0%
CTAAGGATATGGAGTCATTA+TGG + chr1_4:48026899-48026918 None:intergenic 35.0%
!! TTGAAAACGAGAAAGCACTA+AGG + chr1_4:48026916-48026935 None:intergenic 35.0%
!! CTTTCTCGTTTTCAAGCTAT+TGG - chr1_4:48026921-48026940 Msa0166620:intron 35.0%
! TTGGTCACTTTGATTCAATC+AGG - chr1_4:48027060-48027079 Msa0166620:CDS 35.0%
! AAGGGTACATTGCATGAATT+TGG - chr1_4:48027098-48027117 Msa0166620:CDS 35.0%
TTTAGATAGGCAGCATATGT+GGG + chr1_4:48027162-48027181 None:intergenic 35.0%
! TTTTAGATAGGCAGCATATG+TGG + chr1_4:48027163-48027182 None:intergenic 35.0%
!!! TGGGCATCTAAGTTTTAGAT+AGG + chr1_4:48027175-48027194 None:intergenic 35.0%
TATAGAACAGCATTTGGATC+TGG + chr1_4:48027207-48027226 None:intergenic 35.0%
CTTGCATATAGAACAGCATT+TGG + chr1_4:48027213-48027232 None:intergenic 35.0%
!!! CAAGAACATTTTGGAACACA+AGG - chr1_4:48027275-48027294 Msa0166620:CDS 35.0%
TTCACATAAGGTGATGTTCT+TGG + chr1_4:48027336-48027355 None:intergenic 35.0%
AAAAAGATGGCAGCTGATAA+TGG - chr1_4:48027354-48027373 Msa0166620:CDS 35.0%
TCAGAGAATAATCCTCTTAC+CGG - chr1_4:48027417-48027436 Msa0166620:intron 35.0%
TCTAATTTCACCTTGATCTC+CGG + chr1_4:48027439-48027458 None:intergenic 35.0%
ACAGAAATTAGAACGAGGCT+TGG + chr1_4:48025950-48025969 None:intergenic 40.0%
GTAGGAGAAGCAATAGAAAG+AGG + chr1_4:48025975-48025994 None:intergenic 40.0%
GTATAACGAGCATGGAAACA+CGG - chr1_4:48026342-48026361 Msa0166620:CDS 40.0%
CTTCCATGATTGCATAGTCA+AGG - chr1_4:48026387-48026406 Msa0166620:CDS 40.0%
TCACCTTGACTATGCAATCA+TGG + chr1_4:48026393-48026412 None:intergenic 40.0%
! CGCAGCAGAGTTATCATTTT+TGG - chr1_4:48026426-48026445 Msa0166620:CDS 40.0%
ATACTTGAAGTTTCGCATCC+CGG + chr1_4:48026568-48026587 None:intergenic 40.0%
! TTTCTCAACAGCAGCTTTGA+TGG + chr1_4:48026598-48026617 None:intergenic 40.0%
TTGAAATGAAGACAGGACGA+AGG - chr1_4:48026826-48026845 Msa0166620:intron 40.0%
TGAAATGAAGACAGGACGAA+GGG - chr1_4:48026827-48026846 Msa0166620:intron 40.0%
!! ACGAGAAAGCACTAAGGATA+TGG + chr1_4:48026910-48026929 None:intergenic 40.0%
! GTCACTTTGATTCAATCAGG+AGG - chr1_4:48027063-48027082 Msa0166620:CDS 40.0%
ATTGCATGAATTTGGTCCAG+AGG - chr1_4:48027106-48027125 Msa0166620:CDS 40.0%
TTAGATAGGCAGCATATGTG+GGG + chr1_4:48027161-48027180 None:intergenic 40.0%
!! TTGGATCTGGATTTGGTGTT+GGG + chr1_4:48027194-48027213 None:intergenic 40.0%
!! TTTGGATCTGGATTTGGTGT+TGG + chr1_4:48027195-48027214 None:intergenic 40.0%
ACAGCATTTGGATCTGGATT+TGG + chr1_4:48027201-48027220 None:intergenic 40.0%
!! GCTGCCATCTTTTTCACATA+AGG + chr1_4:48027348-48027367 None:intergenic 40.0%
GTTACTTGATCAACCGACAA+AGG - chr1_4:48027602-48027621 Msa0166620:intron 40.0%
ACAGAAATTAGAACGAGGCT+TGG + chr1_4:48025950-48025969 None:intergenic 40.0%
GTAGGAGAAGCAATAGAAAG+AGG + chr1_4:48025975-48025994 None:intergenic 40.0%
GTATAACGAGCATGGAAACA+CGG - chr1_4:48026342-48026361 Msa0166620:CDS 40.0%
CTTCCATGATTGCATAGTCA+AGG - chr1_4:48026387-48026406 Msa0166620:CDS 40.0%
TCACCTTGACTATGCAATCA+TGG + chr1_4:48026393-48026412 None:intergenic 40.0%
! CGCAGCAGAGTTATCATTTT+TGG - chr1_4:48026426-48026445 Msa0166620:CDS 40.0%
ATACTTGAAGTTTCGCATCC+CGG + chr1_4:48026568-48026587 None:intergenic 40.0%
! TTTCTCAACAGCAGCTTTGA+TGG + chr1_4:48026598-48026617 None:intergenic 40.0%
TTGAAATGAAGACAGGACGA+AGG - chr1_4:48026826-48026845 Msa0166620:intron 40.0%
TGAAATGAAGACAGGACGAA+GGG - chr1_4:48026827-48026846 Msa0166620:intron 40.0%
!! ACGAGAAAGCACTAAGGATA+TGG + chr1_4:48026910-48026929 None:intergenic 40.0%
! GTCACTTTGATTCAATCAGG+AGG - chr1_4:48027063-48027082 Msa0166620:CDS 40.0%
ATTGCATGAATTTGGTCCAG+AGG - chr1_4:48027106-48027125 Msa0166620:CDS 40.0%
TTAGATAGGCAGCATATGTG+GGG + chr1_4:48027161-48027180 None:intergenic 40.0%
!! TTGGATCTGGATTTGGTGTT+GGG + chr1_4:48027194-48027213 None:intergenic 40.0%
!! TTTGGATCTGGATTTGGTGT+TGG + chr1_4:48027195-48027214 None:intergenic 40.0%
ACAGCATTTGGATCTGGATT+TGG + chr1_4:48027201-48027220 None:intergenic 40.0%
!! GCTGCCATCTTTTTCACATA+AGG + chr1_4:48027348-48027367 None:intergenic 40.0%
GTTACTTGATCAACCGACAA+AGG - chr1_4:48027602-48027621 Msa0166620:intron 40.0%
GAAATTAGAACGAGGCTTGG+TGG + chr1_4:48025947-48025966 None:intergenic 45.0%
!! TTCTGCTTTTGGGCAGCTTT+TGG + chr1_4:48026293-48026312 None:intergenic 45.0%
CAGACATCAGAGAGAAGTTC+CGG - chr1_4:48026546-48026565 Msa0166620:intron 45.0%
AGACATCAGAGAGAAGTTCC+GGG - chr1_4:48026547-48026566 Msa0166620:intron 45.0%
TCGGCACATGATACTGTCAA+GGG + chr1_4:48026627-48026646 None:intergenic 45.0%
AGCGGAAAGAGCAACAATGT+CGG + chr1_4:48026646-48026665 None:intergenic 45.0%
CATGGCAATTCCATCTCTAG+CGG + chr1_4:48026664-48026683 None:intergenic 45.0%
GTGCGACTCTTAACATACCA+TGG + chr1_4:48026682-48026701 None:intergenic 45.0%
GGTGCATTTGTATAGTTGGG+AGG - chr1_4:48026795-48026814 Msa0166620:CDS 45.0%
AGTACCTAACAGAGCAACAG+TGG + chr1_4:48026961-48026980 None:intergenic 45.0%
!! CATATGTGGGGTCTAGCTTT+GGG + chr1_4:48027149-48027168 None:intergenic 45.0%
GTTCTTCATCCACAGTGAGT+AGG + chr1_4:48027302-48027321 None:intergenic 45.0%
AATCCTCTTACCGGAGATCA+AGG - chr1_4:48027426-48027445 Msa0166620:intron 45.0%
GCATAAAAGTCTAAGCCCGA+TGG + chr1_4:48027556-48027575 None:intergenic 45.0%
GAAATTAGAACGAGGCTTGG+TGG + chr1_4:48025947-48025966 None:intergenic 45.0%
!! TTCTGCTTTTGGGCAGCTTT+TGG + chr1_4:48026293-48026312 None:intergenic 45.0%
CAGACATCAGAGAGAAGTTC+CGG - chr1_4:48026546-48026565 Msa0166620:intron 45.0%
AGACATCAGAGAGAAGTTCC+GGG - chr1_4:48026547-48026566 Msa0166620:intron 45.0%
TCGGCACATGATACTGTCAA+GGG + chr1_4:48026627-48026646 None:intergenic 45.0%
AGCGGAAAGAGCAACAATGT+CGG + chr1_4:48026646-48026665 None:intergenic 45.0%
CATGGCAATTCCATCTCTAG+CGG + chr1_4:48026664-48026683 None:intergenic 45.0%
GTGCGACTCTTAACATACCA+TGG + chr1_4:48026682-48026701 None:intergenic 45.0%
GGTGCATTTGTATAGTTGGG+AGG - chr1_4:48026795-48026814 Msa0166620:CDS 45.0%
AGTACCTAACAGAGCAACAG+TGG + chr1_4:48026961-48026980 None:intergenic 45.0%
!! CATATGTGGGGTCTAGCTTT+GGG + chr1_4:48027149-48027168 None:intergenic 45.0%
GTTCTTCATCCACAGTGAGT+AGG + chr1_4:48027302-48027321 None:intergenic 45.0%
AATCCTCTTACCGGAGATCA+AGG - chr1_4:48027426-48027445 Msa0166620:intron 45.0%
GCATAAAAGTCTAAGCCCGA+TGG + chr1_4:48027556-48027575 None:intergenic 45.0%
TAACGAGCATGGAAACACGG+CGG - chr1_4:48026345-48026364 Msa0166620:CDS 50.0%
!!! CGCATCCCTGCTTTTAGAGT+CGG - chr1_4:48026503-48026522 Msa0166620:CDS 50.0%
!!! TCCCTGCTTTTAGAGTCGGT+AGG - chr1_4:48026507-48026526 Msa0166620:CDS 50.0%
!! GCCTACCGACTCTAAAAGCA+GGG + chr1_4:48026511-48026530 None:intergenic 50.0%
GTCGGTAGGCGATGTAGTAT+CGG - chr1_4:48026521-48026540 Msa0166620:CDS 50.0%
GTCGGCACATGATACTGTCA+AGG + chr1_4:48026628-48026647 None:intergenic 50.0%
GTTGCTCTTTCCGCTAGAGA+TGG - chr1_4:48026651-48026670 Msa0166620:intron 50.0%
CGCTAGAGATGGAATTGCCA+TGG - chr1_4:48026662-48026681 Msa0166620:CDS 50.0%
ATGAAGACAGGACGAAGGGA+TGG - chr1_4:48026831-48026850 Msa0166620:intron 50.0%
GAAGCCACTGTTGCTCTGTT+AGG - chr1_4:48026954-48026973 Msa0166620:CDS 50.0%
TCAATCAGGAGGACACTCAG+TGG - chr1_4:48027074-48027093 Msa0166620:CDS 50.0%
CAATCAGGAGGACACTCAGT+GGG - chr1_4:48027075-48027094 Msa0166620:CDS 50.0%
!! TCTAGCTTTGGGTCCACTGT+GGG + chr1_4:48027138-48027157 None:intergenic 50.0%
! GCATATGTGGGGTCTAGCTT+TGG + chr1_4:48027150-48027169 None:intergenic 50.0%
TCACCTTGATCTCCGGTAAG+AGG + chr1_4:48027432-48027451 None:intergenic 50.0%
TAACGAGCATGGAAACACGG+CGG - chr1_4:48026345-48026364 Msa0166620:CDS 50.0%
!!! CGCATCCCTGCTTTTAGAGT+CGG - chr1_4:48026503-48026522 Msa0166620:CDS 50.0%
!!! TCCCTGCTTTTAGAGTCGGT+AGG - chr1_4:48026507-48026526 Msa0166620:CDS 50.0%
!! GCCTACCGACTCTAAAAGCA+GGG + chr1_4:48026511-48026530 None:intergenic 50.0%
GTCGGTAGGCGATGTAGTAT+CGG - chr1_4:48026521-48026540 Msa0166620:CDS 50.0%
GTCGGCACATGATACTGTCA+AGG + chr1_4:48026628-48026647 None:intergenic 50.0%
GTTGCTCTTTCCGCTAGAGA+TGG - chr1_4:48026651-48026670 Msa0166620:intron 50.0%
CGCTAGAGATGGAATTGCCA+TGG - chr1_4:48026662-48026681 Msa0166620:CDS 50.0%
ATGAAGACAGGACGAAGGGA+TGG - chr1_4:48026831-48026850 Msa0166620:intron 50.0%
GAAGCCACTGTTGCTCTGTT+AGG - chr1_4:48026954-48026973 Msa0166620:CDS 50.0%
TCAATCAGGAGGACACTCAG+TGG - chr1_4:48027074-48027093 Msa0166620:CDS 50.0%
CAATCAGGAGGACACTCAGT+GGG - chr1_4:48027075-48027094 Msa0166620:CDS 50.0%
!! TCTAGCTTTGGGTCCACTGT+GGG + chr1_4:48027138-48027157 None:intergenic 50.0%
! GCATATGTGGGGTCTAGCTT+TGG + chr1_4:48027150-48027169 None:intergenic 50.0%
TCACCTTGATCTCCGGTAAG+AGG + chr1_4:48027432-48027451 None:intergenic 50.0%
ATGGAAACACGGCGGTTTCG+TGG - chr1_4:48026353-48026372 Msa0166620:CDS 55.0%
TGGAAACACGGCGGTTTCGT+GGG - chr1_4:48026354-48026373 Msa0166620:CDS 55.0%
!! CGCCTACCGACTCTAAAAGC+AGG + chr1_4:48026512-48026531 None:intergenic 55.0%
AGGAGGACACTCAGTGGGAA+GGG - chr1_4:48027080-48027099 Msa0166620:CDS 55.0%
!! CTAGCTTTGGGTCCACTGTG+GGG + chr1_4:48027137-48027156 None:intergenic 55.0%
!! GTCTAGCTTTGGGTCCACTG+TGG + chr1_4:48027139-48027158 None:intergenic 55.0%
ACACAAGGGCCTACTCACTG+TGG - chr1_4:48027290-48027309 Msa0166620:CDS 55.0%
ATGGAAACACGGCGGTTTCG+TGG - chr1_4:48026353-48026372 Msa0166620:CDS 55.0%
TGGAAACACGGCGGTTTCGT+GGG - chr1_4:48026354-48026373 Msa0166620:CDS 55.0%
!! CGCCTACCGACTCTAAAAGC+AGG + chr1_4:48026512-48026531 None:intergenic 55.0%
AGGAGGACACTCAGTGGGAA+GGG - chr1_4:48027080-48027099 Msa0166620:CDS 55.0%
!! CTAGCTTTGGGTCCACTGTG+GGG + chr1_4:48027137-48027156 None:intergenic 55.0%
!! GTCTAGCTTTGGGTCCACTG+TGG + chr1_4:48027139-48027158 None:intergenic 55.0%
ACACAAGGGCCTACTCACTG+TGG - chr1_4:48027290-48027309 Msa0166620:CDS 55.0%
CAGGAGGACACTCAGTGGGA+AGG - chr1_4:48027079-48027098 Msa0166620:CDS 60.0%
CCACTGTGGGGTATAGCCTC+TGG + chr1_4:48027125-48027144 None:intergenic 60.0%
CCAGAGGCTATACCCCACAG+TGG - chr1_4:48027122-48027141 Msa0166620:CDS 60.0%
CAGGAGGACACTCAGTGGGA+AGG - chr1_4:48027079-48027098 Msa0166620:CDS 60.0%
CCACTGTGGGGTATAGCCTC+TGG + chr1_4:48027125-48027144 None:intergenic 60.0%
CCAGAGGCTATACCCCACAG+TGG - chr1_4:48027122-48027141 Msa0166620:CDS 60.0%
GGATGCGTCGCATGACTGCG+TGG + chr1_4:48026490-48026509 None:intergenic 65.0%
GGATGCGTCGCATGACTGCG+TGG + chr1_4:48026490-48026509 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 48025912 48027725 48025912 ID=Msa0166620;Name=Msa0166620
chr1_4 mRNA 48025912 48027725 48025912 ID=Msa0166620-mRNA-1;Parent=Msa0166620;Name=Msa0166620-mRNA-1;_AED=0.30;_eAED=0.30;_QI=28|1|1|1|0.6|0.33|6|213|325
chr1_4 exon 48025912 48025986 48025912 ID=Msa0166620-mRNA-1:exon:13984;Parent=Msa0166620-mRNA-1
chr1_4 exon 48026011 48026555 48026011 ID=Msa0166620-mRNA-1:exon:13983;Parent=Msa0166620-mRNA-1
chr1_4 exon 48026662 48026827 48026662 ID=Msa0166620-mRNA-1:exon:13982;Parent=Msa0166620-mRNA-1
chr1_4 exon 48026954 48027142 48026954 ID=Msa0166620-mRNA-1:exon:13981;Parent=Msa0166620-mRNA-1
chr1_4 exon 48027229 48027377 48027229 ID=Msa0166620-mRNA-1:exon:13980;Parent=Msa0166620-mRNA-1
chr1_4 exon 48027631 48027725 48027631 ID=Msa0166620-mRNA-1:exon:13979;Parent=Msa0166620-mRNA-1
chr1_4 five_prime_UTR 48027698 48027725 48027698 ID=Msa0166620-mRNA-1:five_prime_utr;Parent=Msa0166620-mRNA-1
chr1_4 CDS 48027631 48027697 48027631 ID=Msa0166620-mRNA-1:cds;Parent=Msa0166620-mRNA-1
chr1_4 CDS 48027229 48027377 48027229 ID=Msa0166620-mRNA-1:cds;Parent=Msa0166620-mRNA-1
chr1_4 CDS 48026954 48027142 48026954 ID=Msa0166620-mRNA-1:cds;Parent=Msa0166620-mRNA-1
chr1_4 CDS 48026662 48026827 48026662 ID=Msa0166620-mRNA-1:cds;Parent=Msa0166620-mRNA-1
chr1_4 CDS 48026149 48026555 48026149 ID=Msa0166620-mRNA-1:cds;Parent=Msa0166620-mRNA-1
chr1_4 three_prime_UTR 48026011 48026148 48026011 ID=Msa0166620-mRNA-1:three_prime_utr;Parent=Msa0166620-mRNA-1
chr1_4 three_prime_UTR 48025912 48025986 48025912 ID=Msa0166620-mRNA-1:three_prime_utr;Parent=Msa0166620-mRNA-1
Gene Sequence

>Msa0166620

ATGGCCACCAAGCCTCGTTCTAATTTCTGTTTCCTCTTTCTATTGCTTCTCCTACATTTTTATTTGGGAAAAAGCCAGCTACAAATGAACTACTACTCCAAAAGCTGCCCAAAAGCAGAAGAGATCATCAAACAAAAAGTCATTGAACTGTATAACGAGCATGGAAACACGGCGGTTTCGTGGGTTAGAAATCTCTTCCATGATTGCATAGTCAAGTCATGCGACGCATCCCTGCTTTTAGAGTCGGTAGGCGATGTAGTATCGGAGCAGACATCAGAGAGAAGTTCCGGGATGCGAAACTTCAAGTATGTGAAAACCATCAAAGCTGCTGTTGAGAAAGAATGTCCCTTGACAGTATCATGTGCCGACATTGTTGCTCTTTCCGCTAGAGATGGAATTGCCATGTTGGGAGGTCCAAAATTTGAAATGAAGACAGGACGAAGGGATGGCAAAGAAAGCCATGTAACAATGGTAGAAGAATTCATTCCAAACCATAATGACTCCATATCCTTAGTGCTTTCTCGTTTTCAAGCTATTGGTGTTGACGTTGAAGCCACTGTTGCTCTGTTAGGAGGACACTCAGTGGGAAGGGTACATTGCATGAATTTGGTCCAGAGGCTATACCCCACAGTGGACCCAAAGCTAGACCCCACATATGCTGCCTATCTAAAACTTAGATGCCCAACACCAAATCCAGATCCAAATGCTGTTCTATATGCAAGAAATGATAGAAAAACGCCAATGATAATCGACAACAACTACTACAAGAACATTTTGGAACACAAGGGCCTACTCACTGTGGATGAAGAACTAGCTACTGATCCAAGAACATCACCTTATGTGAAAAAGATGGCAGCTGATAATGGATACTTCAATGAACAATTCTCAAGGGCTGTTCAATTGTTATCAGAGAATAATCCTCTTACCGGAGATCAAGGTGAAATTAGAAAGGATTGTCGTTACGTTAATGCCAATTAG
Protein sequence

>Msa0166620

MATKPRSNFCFLFLLLLLHFYLGKSQLQMNYYSKSCPKAEEIIKQKVIELYNEHGNTAVSWVRNLFHDCIVKSCDASLLLESVGDVVSEQTSERSSGMRNFKYVKTIKAAVEKECPLTVSCADIVALSARDGIAMLGGPKFEMKTGRRDGKESHVTMVEEFIPNHNDSISLVLSRFQAIGVDVEATVALLGGHSVGRVHCMNLVQRLYPTVDPKLDPTYAAYLKLRCPTPNPDPNAVLYARNDRKTPMIIDNNYYKNILEHKGLLTVDEELATDPRTSPYVKKMAADNGYFNEQFSRAVQLLSENNPLTGDQGEIRKDCRYVNAN*