AlfalfaGEDB | Msa1314810

M. sativa cultivar zhongmu-4 / Msa1314810

KEGG: K00454|K15718
GO: -
PFAM: PF00305.22

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1314810	XP_024627820.2	93.822	259	16	0	200	458	151	409	7.88e-177	511

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1314810	sp\|P38414\|LOX1_LENCU	80.695	259	50	0	200	458	608	866	5.02e-151	454
Msa1314810	sp\|P38414\|LOX1_LENCU	80.435	92	18	0	116	207	269	360	2.03e-42	164

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1314810	A0A396GDH7	59.292	113	28	3	138	244	8	108	9.51e-25	117
Msa1314810	A0A396GDH7	93.822	259	16	0	200	458	203	461	3.39e-176	511

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000180	Msa1314810	-0.814259	1.687828e-51	-8.615850e-47
Msa0011510	Msa1314810	0.802571	5.266706e-49	-8.615850e-47
Msa0021240	Msa1314810	-0.815042	1.132157e-51	-8.615850e-47
Msa0022230	Msa1314810	-0.818921	1.522598e-52	-8.615850e-47
Msa0025060	Msa1314810	-0.828796	7.368138e-55	-8.615850e-47
Msa0025090	Msa1314810	-0.825539	4.436449e-54	-8.615850e-47
Msa0028450	Msa1314810	-0.814797	1.282682e-51	-8.615850e-47
Msa0028940	Msa1314810	-0.800928	1.144671e-48	-8.615850e-47
Msa0030760	Msa1314810	-0.801231	9.927116e-49	-8.615850e-47
Msa0033190	Msa1314810	-0.813576	2.386947e-51	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1314810	MtrunA17_Chr8g0344241	93.822	259	16	0	200	458	203	461	6.53e-180	511
Msa1314810	MtrunA17_Chr8g0344241	59.292	113	28	3	138	244	8	108	1.83e-28	117
Msa1314810	MtrunA17_Chr8g0344211	94.981	259	13	0	200	458	610	868	8.71e-177	517
Msa1314810	MtrunA17_Chr8g0344211	100.000	92	0	0	116	207	271	362	3.19e-53	191
Msa1314810	MtrunA17_Chr8g0342971	84.556	259	40	0	200	458	612	870	8.97e-159	471
Msa1314810	MtrunA17_Chr8g0342971	77.174	92	21	0	116	207	273	364	6.85e-41	155
Msa1314810	MtrunA17_Chr8g0342951	83.784	259	42	0	200	458	610	868	6.75e-155	461
Msa1314810	MtrunA17_Chr8g0342951	82.609	92	16	0	116	207	271	362	2.76e-43	163
Msa1314810	MtrunA17_Chr8g0343011	82.937	252	43	0	207	458	614	865	6.29e-151	451
Msa1314810	MtrunA17_Chr8g0343011	62.264	106	30	3	103	207	263	359	3.83e-32	130

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1314810	AT1G55020.1	57.143	252	107	1	207	458	609	859	7.99e-100	318
Msa1314810	AT1G55020.1	43.750	96	48	2	116	207	261	354	4.75e-16	81.3
Msa1314810	AT3G22400.1	56.061	264	110	2	200	458	624	886	5.28e-99	316
Msa1314810	AT3G22400.1	43.750	96	48	2	116	207	283	376	1.32e-15	80.1
Msa1314810	AT1G72520.1	43.223	273	152	1	186	458	657	926	2.99e-75	253
Msa1314810	AT1G17420.1	43.223	273	152	1	186	458	650	919	4.20e-73	248
Msa1314810	AT1G67560.1	40.876	274	158	2	186	458	647	917	2.86e-64	223
Msa1314810	AT3G45140.2	39.209	278	159	4	185	458	483	754	1.82e-60	211
Msa1314810	AT3G45140.1	39.209	278	159	4	185	458	625	896	5.07e-60	211
Msa1314810	AT3G14060.1	42.478	113	61	3	4	115	16	125	4.63e-17	77.8

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Find 105 sgRNAs with CRISPR-Local

Find 259 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACAGGCCTACAAGAAGTT+TGG	0.372935	8_4:-13925930	Msa1314810:CDS
AAACCACGGCGGAGATTAAT+TGG	0.312099	8_4:-13929597	Msa1314810:CDS
AACCTTCCGCCGTCTGAATA+TGG	0.240508	8_4:+13929644	None:intergenic
AACTTCTTGTAGGCCTGTTT+TGG	0.254785	8_4:+13925933	None:intergenic
AAGCTTGCACCATTCTCATA+TGG	0.416322	8_4:-13926238	Msa1314810:CDS
AAGGTTCAAACGGTGAGGCT+AGG	0.576644	8_4:-13929627	Msa1314810:CDS
AATATCTTAGGTTATTGAAA+CGG	0.511068	8_4:-13929524	Msa1314810:CDS
AATATGGAGACCTTGCGGCG+GGG	0.701594	8_4:+13929660	None:intergenic
ACAACGGTATTGGGCTAATC+TGG	0.354078	8_4:+13927375	None:intergenic
ACTTATCTACTTATCCTTCT+CGG	0.384698	8_4:-13929386	Msa1314810:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
GGCTTAAGTTACAATATCTT+AGG	-	chr8_4:13925909-13925928	Msa1314810:CDS	30.0%
TAGATAAGTTAACACCAAGA+GGG	+	chr8_4:13926048-13926067	None:intergenic	30.0%
ACTTATCTACTTATCCTTCT+CGG	-	chr8_4:13926059-13926078	Msa1314810:CDS	30.0%
CTGAGAGGTGAAAAAATATA+TGG	+	chr8_4:13926385-13926404	None:intergenic	30.0%
TCAGTCTCTAATATTGTTCA+TGG	-	chr8_4:13926401-13926420	Msa1314810:CDS	30.0%
AGATAGGAGATATTCTTGTA+TGG	-	chr8_4:13926582-13926601	Msa1314810:intron	30.0%
TATCTTATTGTGTGCCTAAA+CGG	+	chr8_4:13926625-13926644	None:intergenic	30.0%
AAGTTAAAAATTGGTTAGGG+AGG	-	chr8_4:13926876-13926895	Msa1314810:intron	30.0%
GTGAGTCAATATTTGTTTGA+TGG	-	chr8_4:13926950-13926969	Msa1314810:intron	30.0%
CTATACGTAAGTTTCAATTC+TGG	-	chr8_4:13927014-13927033	Msa1314810:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_4	gene	13925770	13929697	13925770	ID=Msa1314810;Name=Msa1314810
chr8_4	mRNA	13925770	13929697	13925770	ID=Msa1314810-mRNA-1;Parent=Msa1314810;Name=Msa1314810-mRNA-1;_AED=0.13;_eAED=0.13;_QI=0\|0\|0\|1\|1\|1\|3\|0\|458
chr8_4	exon	13929354	13929697	13929354	ID=Msa1314810-mRNA-1:exon:5247;Parent=Msa1314810-mRNA-1
chr8_4	exon	13927309	13927586	13927309	ID=Msa1314810-mRNA-1:exon:5246;Parent=Msa1314810-mRNA-1
chr8_4	exon	13925770	13926524	13925770	ID=Msa1314810-mRNA-1:exon:5245;Parent=Msa1314810-mRNA-1
chr8_4	CDS	13929354	13929697	13929354	ID=Msa1314810-mRNA-1:cds;Parent=Msa1314810-mRNA-1
chr8_4	CDS	13927309	13927586	13927309	ID=Msa1314810-mRNA-1:cds;Parent=Msa1314810-mRNA-1
chr8_4	CDS	13925770	13926524	13925770	ID=Msa1314810-mRNA-1:cds;Parent=Msa1314810-mRNA-1

Gene Sequence

>Msa1314810

ATGACAACATGGCAGCCCCGCCGCAAGGTCTCCATATTCAGACGGCGGAAGGTTCAAACGGTGAGGCTAGGTAGCAAGAAACCACGGCGGAGATTAATTGGTGGCATAGTTAGAATGTTTAAAAGGATGCGTGTAAAGTGGCTTAAGTTACAATATCTTAGGTTATTGAAACGGCTTAAGGAACATTATAGAAACGTTGTGAAAGATCTTATTGAAGCTGGTGCTACCATTGAAACTTTTCAACAACGCCTTTTTATGGAAAGCACTTTTGCTGTCCCTCTTGGTGTTAACTTATCTACTTATCCTTCTCGGTTTGGTTCAGATTATCCTCGAACCCTCTTCATAGATGAATCATTTGGTCACTTGAAGTCATCTGACTTTCTTGGTTATGTACTCAAATCAGCAACTCACAGAGTCATACCGAATTTGAGATCTAAAGCTACATTACAACTCAATAACCCTGAGTTTAACACCTTTGAAGATATACGCTCTCTCTACGATGGTGGAATTAAGCTTCCTACTGATGTACTAAGCCAGATTAGCCCAATACCGTTGTTCAAAGAACTTTTTCGAACTGATGGTGAAGCGGTACTTAAGTTTCCACCACCTAAAGTGGTTCAAGGAGCGGCAGTTCAGGATCCAGCTTCTCCACACGGTGTTCGACTTCTGATAGAGGACTATCCTTATGCTTCAGATGGACTAGAGATATGGGCTGCTATTAAGTCATGGGTTGATGAATATGTGAATTTCTACTACAAGTCTGATGCAGATGTTGTAAAAGATTCCGAACTCCAAGCATTTTGGAAAGAACTTGTCGAGGTGGGTCATGGTGACTTTAGGAATGCAACATGGTGGTTTAAGATGCAAACTCGTACGGAGTTGAAAGAAGCTTGCACCATTCTCATATGGATTTCTTCAGCGCTTCATGCAGCTGTTAATTTTGGACAATATGCGTATGGAGGATATATCCTTAACCGACCAACAAAAAGTAGAAGATTCATGCCTGAGAAAGGATCTGTTGAGTATGATGAGCTTGCTAAGGACTACCAGAAGACTTATTTGAGAACAATCACCGCGAAGAATGATACCCTTACTAACTTGACCATCTTAGAGGTCTTGTCAAGGCATGCTTCTGATGAGCAATACCTTGGAGAGAGAATCGAAGGCGATCTCTGGACTTCTGATTCACAGCCAAAACAGGCCTACAAGAAGTTTGGAAAGAAGTTGGCTGACATCGAGAAAAACCTCATTCAAAGGAACAATGATGAAACACTAAGGAATCGAAATGGACCTGTGAAGATGCCATATACTATGCTTTATCCTTCTAGTGAGGAAGGATTGACTTCCAGAGGCATTCCAAACAGTGTCTCCATCTAA

Protein sequence

>Msa1314810

MTTWQPRRKVSIFRRRKVQTVRLGSKKPRRRLIGGIVRMFKRMRVKWLKLQYLRLLKRLKEHYRNVVKDLIEAGATIETFQQRLFMESTFAVPLGVNLSTYPSRFGSDYPRTLFIDESFGHLKSSDFLGYVLKSATHRVIPNLRSKATLQLNNPEFNTFEDIRSLYDGGIKLPTDVLSQISPIPLFKELFRTDGEAVLKFPPPKVVQGAAVQDPASPHGVRLLIEDYPYASDGLEIWAAIKSWVDEYVNFYYKSDADVVKDSELQAFWKELVEVGHGDFRNATWWFKMQTRTELKEACTILIWISSALHAAVNFGQYAYGGYILNRPTKSRRFMPEKGSVEYDELAKDYQKTYLRTITAKNDTLTNLTILEVLSRHASDEQYLGERIEGDLWTSDSQPKQAYKKFGKKLADIEKNLIQRNNDETLRNRNGPVKMPYTMLYPSSEEGLTSRGIPNSVSI*