AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0833230

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0833230 MtrunA17_Chr5g0398531 97.846 325 3 2 1 321 1 325 0.0 630
Msa0833230 MtrunA17_Chr8g0351651 58.982 334 83 12 1 314 1 300 4.47e-90 271
Msa0833230 MtrunA17_Chr5g0443311 62.727 220 69 2 41 259 5 212 2.94e-78 239
Msa0833230 MtrunA17_Chr1g0168121 73.885 157 41 0 91 247 35 191 1.11e-68 215
Msa0833230 MtrunA17_Chr1g0190051 65.761 184 62 1 92 275 68 250 1.28e-68 216
Msa0833230 MtrunA17_Chr7g0250031 68.874 151 47 0 107 257 66 216 1.13e-65 207
Msa0833230 MtrunA17_Chr3g0112131 53.125 224 78 4 108 314 89 302 2.65e-65 208
Msa0833230 MtrunA17_Chr4g0054651 61.275 204 70 2 47 250 34 228 5.69e-65 206
Msa0833230 MtrunA17_Chr3g0133051 50.802 187 86 3 91 273 67 251 3.84e-58 189
Msa0833230 MtrunA17_Chr5g0435681 52.358 212 95 2 40 247 29 238 2.06e-54 180
Msa0833230 MtrunA17_Chr5g0427311 46.018 226 94 6 30 250 6 208 6.91e-48 160
Msa0833230 MtrunA17_Chr1g0186441 42.132 197 60 3 91 279 58 208 7.00e-36 130
Msa0833230 MtrunA17_Chr3g0094471 41.040 173 76 2 98 244 55 227 7.93e-32 120
Msa0833230 MtrunA17_Chr5g0441851 39.456 147 78 2 97 232 76 222 1.96e-30 115
Msa0833230 MtrunA17_Chr4g0028181 30.918 207 133 3 97 293 50 256 1.96e-24 100
Msa0833230 MtrunA17_Chr5g0397551 37.427 171 94 5 98 256 104 273 3.11e-24 100
Msa0833230 MtrunA17_Chr5g0441901 35.333 150 87 2 97 237 74 222 3.51e-23 95.9
Msa0833230 MtrunA17_Chr4g0028211 34.161 161 96 3 97 247 35 195 4.87e-23 95.5
Msa0833230 MtrunA17_Chr1g0161621 35.329 167 92 5 93 245 96 260 3.06e-22 94.7
Msa0833230 MtrunA17_Chr1g0175001 32.099 162 101 2 95 247 82 243 9.50e-22 93.2
Msa0833230 MtrunA17_Chr5g0441441 37.162 148 87 1 98 239 50 197 3.24e-21 90.5
Msa0833230 MtrunA17_Chr1g0179461 41.600 125 69 2 128 248 148 272 4.97e-21 92.4
Msa0833230 MtrunA17_Chr1g0190011 35.833 120 76 1 120 238 104 223 1.42e-19 87.8
Msa0833230 MtrunA17_Chr6g0476061 42.241 116 64 2 125 237 130 245 2.13e-19 87.0
Msa0833230 MtrunA17_Chr4g0054661 35.583 163 80 5 87 237 107 256 1.89e-18 84.7
Msa0833230 MtrunA17_Chr1g0188211 39.264 163 77 5 95 237 88 248 2.83e-18 84.3
Msa0833230 MtrunA17_Chr1g0186461 42.000 150 39 4 98 247 52 153 3.03e-18 81.3
Msa0833230 MtrunA17_Chr8g0386741 38.710 124 72 3 117 237 158 280 6.92e-18 83.2
Msa0833230 MtrunA17_Chr8g0351761 43.860 114 60 1 128 237 145 258 1.02e-16 79.7
Msa0833230 MtrunA17_Chr4g0034501 38.542 96 59 0 97 192 33 128 2.01e-16 75.9
Msa0833230 MtrunA17_Chr7g0247431 30.769 169 108 3 98 260 127 292 3.79e-16 78.2
Msa0833230 MtrunA17_Chr7g0274821 37.288 118 74 0 120 237 138 255 4.27e-16 78.2
Msa0833230 MtrunA17_Chr7g0245841 49.254 67 34 0 128 194 130 196 8.45e-16 77.0
Msa0833230 MtrunA17_Chr8g0374021 34.507 142 71 4 129 251 5 143 5.98e-15 71.2
Msa0833230 MtrunA17_Chr8g0349091 37.671 146 85 2 98 237 125 270 2.49e-14 72.8
Msa0833230 MtrunA17_Chr4g0056761 49.254 67 34 0 128 194 147 213 1.28e-13 70.5
Msa0833230 MtrunA17_Chr5g0398621 32.258 124 77 2 128 244 100 223 1.67e-13 69.3
Msa0833230 MtrunA17_Chr4g0027351 41.346 104 59 2 128 229 22 125 2.67e-13 66.2
Msa0833230 MtrunA17_Chr4g0027331 38.983 118 62 3 128 237 22 137 2.42e-12 63.5
Msa0833230 MtrunA17_Chr4g0027371 38.793 116 65 2 128 237 22 137 3.23e-12 65.5
Msa0833230 MtrunA17_Chr6g0476241 35.135 111 70 2 128 238 180 288 2.36e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0833230 AT4G22810.1 55.429 350 101 12 1 321 1 324 1.55e-79 245
Msa0833230 AT2G45430.1 56.250 336 113 11 1 321 1 317 2.74e-75 234
Msa0833230 AT4G12050.1 64.469 273 74 6 1 251 1 272 3.83e-75 234
Msa0833230 AT2G42940.1 68.293 164 52 0 96 259 51 214 1.52e-71 222
Msa0833230 AT2G35270.1 69.872 156 47 0 96 251 76 231 6.04e-71 222
Msa0833230 AT3G60870.1 57.082 233 79 5 91 321 52 265 1.56e-69 218
Msa0833230 AT4G14465.1 54.894 235 89 5 91 321 60 281 2.24e-68 215
Msa0833230 AT1G76500.1 65.244 164 48 1 95 249 69 232 2.98e-63 202
Msa0833230 AT3G04570.1 69.697 165 42 1 91 247 73 237 3.98e-63 202
Msa0833230 AT4G17800.1 63.584 173 51 1 107 267 91 263 4.89e-63 202
Msa0833230 AT5G49700.1 47.449 196 88 4 96 281 54 244 1.39e-56 184
Msa0833230 AT4G35390.1 72.667 150 39 1 98 245 63 212 2.80e-55 182
Msa0833230 AT3G55560.1 64.583 144 50 1 105 247 95 238 4.47e-50 169
Msa0833230 AT1G20900.1 67.296 159 43 1 98 247 86 244 5.16e-50 169
Msa0833230 AT1G14490.2 44.751 181 91 3 99 272 25 203 6.38e-41 142
Msa0833230 AT1G14490.1 44.751 181 91 3 99 272 6 184 1.03e-40 141
Msa0833230 AT1G63470.1 42.466 146 77 3 98 237 147 291 3.62e-25 104
Msa0833230 AT5G62260.2 40.000 140 78 3 111 245 145 283 2.92e-23 97.8
Msa0833230 AT5G62260.1 42.424 132 70 3 111 237 145 275 1.54e-22 97.4
Msa0833230 AT5G62260.3 42.424 132 70 3 111 237 145 275 1.54e-22 97.4
Msa0833230 AT5G51590.1 43.363 113 61 1 128 237 181 293 6.73e-22 95.9
Msa0833230 AT4G12080.1 41.593 113 63 1 128 237 173 285 3.26e-21 93.2
Msa0833230 AT2G33620.1 40.260 154 82 3 87 237 132 278 3.99e-21 92.8
Msa0833230 AT2G33620.4 40.260 154 82 3 87 237 132 278 3.99e-21 92.8
Msa0833230 AT2G33620.2 40.260 154 82 3 87 237 132 278 3.99e-21 92.8
Msa0833230 AT2G33620.3 40.260 154 82 3 87 237 132 278 3.99e-21 92.8
Msa0833230 AT4G25320.1 41.593 113 63 1 128 237 169 281 8.62e-21 92.4
Msa0833230 AT4G17950.1 37.821 156 90 3 87 237 185 338 1.44e-20 92.0
Msa0833230 AT3G04590.2 36.364 110 70 0 128 237 174 283 7.40e-20 89.7
Msa0833230 AT3G04590.1 36.364 110 70 0 128 237 174 283 2.13e-19 87.4
Msa0833230 AT2G45850.1 36.538 156 81 5 98 241 132 281 2.89e-18 84.7
Msa0833230 AT2G45850.2 36.538 156 81 5 98 241 132 281 2.89e-18 84.7
Msa0833230 AT1G63480.5 30.846 201 117 5 98 279 124 321 6.47e-18 83.6
Msa0833230 AT1G63480.7 30.846 201 117 5 98 279 124 321 6.47e-18 83.6
Msa0833230 AT1G63480.4 30.846 201 117 5 98 279 124 321 6.47e-18 83.6
Msa0833230 AT1G63480.3 30.846 201 117 5 98 279 124 321 6.47e-18 83.6
Msa0833230 AT1G63480.6 30.846 201 117 5 98 279 124 321 6.47e-18 83.6
Msa0833230 AT1G63480.2 30.846 201 117 5 98 279 130 327 7.86e-18 83.6
Msa0833230 AT1G63480.1 30.846 201 117 5 98 279 130 327 7.86e-18 83.6
Msa0833230 AT2G45850.3 35.948 153 80 5 98 238 132 278 3.13e-17 80.9
Msa0833230 AT4G00200.2 43.363 113 61 1 128 237 126 238 1.26e-15 76.6
Msa0833230 AT4G00200.1 43.363 113 61 1 128 237 126 238 1.26e-15 76.6
Msa0833230 AT5G46640.1 38.514 148 86 2 93 237 150 295 1.59e-13 71.2
Msa0833230 AT4G22770.2 38.938 113 66 1 128 237 153 265 2.03e-13 70.5
Msa0833230 AT4G22770.1 38.938 113 66 1 128 237 153 265 2.03e-13 70.5
Msa0833230 AT3G61310.1 32.877 146 92 2 98 237 134 279 2.05e-12 67.4

Find 93 sgRNAs with CRISPR-Local

Find 198 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTGGTGCTTTGATTGCTTC+TGG 0.209013 5_4:-6386242 Msa0833230:CDS
CCTGCTCCGCCCGCTGCTTC+TGG 0.230880 5_4:-6386317 Msa0833230:CDS
GTTGCTGCTGTTGGTGTTGA+TGG 0.289751 5_4:+6386831 None:intergenic
TTAAGATTAGGATTAGAATT+AGG 0.294967 5_4:+6386023 None:intergenic
GGAGCTGTTGTTACACTTCA+TGG 0.313521 5_4:-6386383 Msa0833230:CDS
GTGATAATAATCGGTGGTTT+TGG 0.328875 5_4:+6386572 None:intergenic
AGATGCCTTCTGATAATTTC+TGG 0.330543 5_4:-6385983 Msa0833230:CDS
GTTGTTGGTGGAAGTGTTGT+TGG 0.349227 5_4:-6386260 Msa0833230:CDS
CCAGAAGCAGCGGGCGGAGC+AGG 0.354965 5_4:+6386317 None:intergenic
GATCCAGCTGGTCTTCCTCT+TGG 0.365755 5_4:+6386605 None:intergenic
TTCGAGATTCTTTCGCTGGC+TGG 0.370912 5_4:-6386356 Msa0833230:CDS
TTGTCCGCCCGCTAAATATA+TGG 0.390502 5_4:+6386289 None:intergenic
ACCTCCTCCGGATTCTAACT+TGG 0.395394 5_4:+6386664 None:intergenic
AATCTTAATCCTAATCTTCT+CGG 0.405395 5_4:-6386008 Msa0833230:CDS
GAATCCGGAGGAGGTGGTTC+CGG 0.408435 5_4:-6386656 Msa0833230:CDS
GGCGGACAAGGGCAGGTTGT+TGG 0.415663 5_4:-6386275 Msa0833230:CDS
GCAGCGGGCGGAGCAGGTGG+TGG 0.424575 5_4:+6386323 None:intergenic
TGCTTATGAGAGACTTCCTT+TGG 0.425099 5_4:-6386183 Msa0833230:CDS
AGAAGATTAGGATTAAGATT+AGG 0.426727 5_4:+6386011 None:intergenic
TCTTCTATTCAACAACTTCA+AGG 0.427876 5_4:-6386146 Msa0833230:CDS
CTAAGGCAACCGGCTTCTCC+TGG 0.438533 5_4:-6386404 Msa0833230:CDS
GGTGGTTCTCCAAGTGGTGG+TGG 0.441214 5_4:-6386113 Msa0833230:CDS
GGTGCTGTTGTTGCTGCTGT+TGG 0.446147 5_4:+6386822 None:intergenic
CTTCCTTTGGAAGATGATGA+TGG 0.447229 5_4:-6386170 Msa0833230:CDS
CTTCAAGGTGGTGGTGGTGG+TGG 0.447916 5_4:-6386131 Msa0833230:CDS
TCTGATAATTTCTGGCCAAC+CGG 0.452010 5_4:-6385975 Msa0833230:CDS
CCTCCTCCGGATTCTAACTT+GGG 0.455968 5_4:+6386665 None:intergenic
AGCGGAAGTAGCAGCGGCGG+TGG 0.456861 5_4:-6386746 Msa0833230:CDS
GGCATCTGACCGAGAAGATT+AGG 0.459191 5_4:+6385999 None:intergenic
GCTGTAAAGTGTGGAATTGA+TGG 0.472588 5_4:+6386801 None:intergenic
GGTTCTCCAAGTGGTGGTGG+TGG 0.480891 5_4:-6386110 Msa0833230:CDS
AAGGTTCGAGATTCTTTCGC+TGG 0.482991 5_4:-6386360 Msa0833230:CDS
CAACTTCAAGGTGGTGGTGG+TGG 0.483443 5_4:-6386134 Msa0833230:CDS
GAAGCAGACATAATAACAAC+AGG 0.487835 5_4:+6386218 None:intergenic
AAACGTGACACTAAGGCAAC+CGG 0.494419 5_4:-6386414 Msa0833230:CDS
TTTAGCCCCAAGTTAGAATC+CGG 0.494865 5_4:-6386671 Msa0833230:CDS
TGATGCAAACGCCTCTTTGA+CGG 0.495629 5_4:+6386459 None:intergenic
ATATATTTAGCGGGCGGACA+AGG 0.504387 5_4:-6386287 Msa0833230:CDS
AGACCAAGAGGAAGACCAGC+TGG 0.509576 5_4:-6386608 Msa0833230:CDS
AGATTAGGATTAGAATTAGG+AGG 0.514675 5_4:+6386026 None:intergenic
CGCCTTGAAAACTCATGTGA+TGG 0.515478 5_4:-6386534 Msa0833230:CDS
CATTGAATCCGTGTCGCCAC+CGG 0.518639 5_4:+6386637 None:intergenic
GCCACCGGAACCACCTCCTC+CGG 0.519443 5_4:+6386652 None:intergenic
TGGTTTGACCATATATTTAG+CGG 0.520577 5_4:-6386297 Msa0833230:CDS
GTTTGCATCATGAGTGGGAC+AGG 0.526109 5_4:-6386446 Msa0833230:CDS
GGTTTGACCATATATTTAGC+GGG 0.527226 5_4:-6386296 Msa0833230:CDS
TTTAGCGGGCGGACAAGGGC+AGG 0.528930 5_4:-6386282 Msa0833230:CDS
CAATATCATCATGATCAATA+CGG 0.533673 5_4:+6385945 None:intergenic
AAGTTAGAATCCGGAGGAGG+TGG 0.540145 5_4:-6386662 Msa0833230:CDS
TGAAGTGTAACAACAGCTCC+AGG 0.541310 5_4:+6386386 None:intergenic
TGATCAATACGGAGAACGAC+CGG 0.541431 5_4:+6385956 None:intergenic
CAACAACTTCAAGGTGGTGG+TGG 0.543583 5_4:-6386137 Msa0833230:CDS
CTGTCACGTGTGATAATAAT+CGG 0.545674 5_4:+6386563 None:intergenic
AGGTGGTTCCGGTGGCGACA+CGG 0.546707 5_4:-6386645 Msa0833230:CDS
TTTGCTAGACGCCGTCAAAG+AGG 0.554223 5_4:-6386470 Msa0833230:CDS
TTAGGATTAGAATTAGGAGG+AGG 0.554265 5_4:+6386029 None:intergenic
TCTCTTGCTGTGTGGAAAGG+TGG 0.554885 5_4:+6386866 None:intergenic
GGACAAGGGCAGGTTGTTGG+TGG 0.555291 5_4:-6386272 Msa0833230:CDS
GAAGCAGCGGGCGGAGCAGG+TGG 0.558808 5_4:+6386320 None:intergenic
ATTCAACAACTTCAAGGTGG+TGG 0.559910 5_4:-6386140 Msa0833230:CDS
TCCGGAGGAGGTGGTTCCGG+TGG 0.560060 5_4:-6386653 Msa0833230:CDS
ACAACAGCTCCAGGAGAAGC+CGG 0.562958 5_4:+6386395 None:intergenic
GGTGGTGGTGGTTCTCCAAG+TGG 0.570648 5_4:-6386119 Msa0833230:CDS
GTTGTTGCTGCTGTAAAGTG+TGG 0.572769 5_4:+6386792 None:intergenic
CGGTTACAAACGTGACACTA+AGG 0.574287 5_4:-6386421 Msa0833230:CDS
CTGTTGTTACACTTCATGGA+AGG 0.580978 5_4:-6386379 Msa0833230:CDS
CCCAAGTTAGAATCCGGAGG+AGG 0.587598 5_4:-6386665 Msa0833230:CDS
ATGGTCAAACCAGAAGCAGC+GGG 0.592455 5_4:+6386308 None:intergenic
GAGGCGTTTGCATCATGAGT+GGG 0.596897 5_4:-6386451 Msa0833230:CDS
CATCATGAGTGGGACAGGAA+CGG 0.597354 5_4:-6386441 Msa0833230:CDS
CTTGCTGTGTGGAAAGGTGG+AGG 0.605189 5_4:+6386869 None:intergenic
TATATTTAGCGGGCGGACAA+GGG 0.608521 5_4:-6386286 Msa0833230:CDS
GGTGGTGGTTCTCCAAGTGG+TGG 0.613666 5_4:-6386116 Msa0833230:CDS
AGATCTCTTGCTGTGTGGAA+AGG 0.616128 5_4:+6386863 None:intergenic
GAACCATCATCATCTTCCAA+AGG 0.621966 5_4:+6386167 None:intergenic
CTCCTCCGGATTCTAACTTG+GGG 0.622134 5_4:+6386666 None:intergenic
CAAAGCGGAAGTAGCAGCGG+CGG 0.624512 5_4:-6386749 Msa0833230:CDS
AGAGGCGTTTGCATCATGAG+TGG 0.639567 5_4:-6386452 Msa0833230:CDS
CAATACGGAGAACGACCGGT+TGG 0.641650 5_4:+6385960 None:intergenic
GTCAAACCAGAAGCAGCGGG+CGG 0.648457 5_4:+6386311 None:intergenic
AGCCCCAAGTTAGAATCCGG+AGG 0.649404 5_4:-6386668 Msa0833230:CDS
TCAATGACAAGAAGACCAAG+AGG 0.650544 5_4:-6386620 Msa0833230:CDS
TATGGTCAAACCAGAAGCAG+CGG 0.651114 5_4:+6386307 None:intergenic
ATGGATCAAATAACATCACA+TGG 0.651930 5_4:-6386902 Msa0833230:CDS
GAACAAAGCGGAAGTAGCAG+CGG 0.653465 5_4:-6386752 Msa0833230:CDS
GTTGGCCAGAAATTATCAGA+AGG 0.660128 5_4:+6385978 None:intergenic
GATGAAGATCTCTTGCTGTG+TGG 0.664521 5_4:+6386858 None:intergenic
TTGACCATATATTTAGCGGG+CGG 0.665260 5_4:-6386293 Msa0833230:CDS
TCTATTCAACAACTTCAAGG+TGG 0.668992 5_4:-6386143 Msa0833230:CDS
CTTGAAAACTCATGTGATGG+AGG 0.692082 5_4:-6386531 Msa0833230:CDS
ACAGATCAAGATGAACAAAG+CGG 0.697061 5_4:-6386764 Msa0833230:CDS
TCACGTGTGATAATAATCGG+TGG 0.702591 5_4:+6386566 None:intergenic
CATGTGATGGAGGTTGCAGA+CGG 0.715960 5_4:-6386521 Msa0833230:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAAGATTAGGATTAGAATT+AGG + chr5_4:6386841-6386860 None:intergenic 20.0%
!! TTAAGATTAGGATTAGAATT+AGG + chr5_4:6386841-6386860 None:intergenic 20.0%
! AGAAGATTAGGATTAAGATT+AGG + chr5_4:6386853-6386872 None:intergenic 25.0%
! AATCTTAATCCTAATCTTCT+CGG - chr5_4:6386853-6386872 Msa0833230:CDS 25.0%
! AGAAGATTAGGATTAAGATT+AGG + chr5_4:6386853-6386872 None:intergenic 25.0%
! AATCTTAATCCTAATCTTCT+CGG - chr5_4:6386853-6386872 Msa0833230:CDS 25.0%
ATGGATCAAATAACATCACA+TGG - chr5_4:6385959-6385978 Msa0833230:CDS 30.0%
TGGTTTGACCATATATTTAG+CGG - chr5_4:6386564-6386583 Msa0833230:CDS 30.0%
TCTTCTATTCAACAACTTCA+AGG - chr5_4:6386715-6386734 Msa0833230:CDS 30.0%
AGATTAGGATTAGAATTAGG+AGG + chr5_4:6386838-6386857 None:intergenic 30.0%
ATGGATCAAATAACATCACA+TGG - chr5_4:6385959-6385978 Msa0833230:CDS 30.0%
TGGTTTGACCATATATTTAG+CGG - chr5_4:6386564-6386583 Msa0833230:CDS 30.0%
TCTTCTATTCAACAACTTCA+AGG - chr5_4:6386715-6386734 Msa0833230:CDS 30.0%
AGATTAGGATTAGAATTAGG+AGG + chr5_4:6386838-6386857 None:intergenic 30.0%
ACAGATCAAGATGAACAAAG+CGG - chr5_4:6386097-6386116 Msa0833230:CDS 35.0%
!!! TGTTGTTGCTGTTTTCTTCT+CGG + chr5_4:6386156-6386175 None:intergenic 35.0%
! GTGATAATAATCGGTGGTTT+TGG + chr5_4:6386292-6386311 None:intergenic 35.0%
CTGTCACGTGTGATAATAAT+CGG + chr5_4:6386301-6386320 None:intergenic 35.0%
GGTTTGACCATATATTTAGC+GGG - chr5_4:6386565-6386584 Msa0833230:CDS 35.0%
GAAGCAGACATAATAACAAC+AGG + chr5_4:6386646-6386665 None:intergenic 35.0%
TCTATTCAACAACTTCAAGG+TGG - chr5_4:6386718-6386737 Msa0833230:CDS 35.0%
TTAGGATTAGAATTAGGAGG+AGG + chr5_4:6386835-6386854 None:intergenic 35.0%
AGATGCCTTCTGATAATTTC+TGG - chr5_4:6386878-6386897 Msa0833230:CDS 35.0%
ACAGATCAAGATGAACAAAG+CGG - chr5_4:6386097-6386116 Msa0833230:CDS 35.0%
!!! TGTTGTTGCTGTTTTCTTCT+CGG + chr5_4:6386156-6386175 None:intergenic 35.0%
! GTGATAATAATCGGTGGTTT+TGG + chr5_4:6386292-6386311 None:intergenic 35.0%
CTGTCACGTGTGATAATAAT+CGG + chr5_4:6386301-6386320 None:intergenic 35.0%
GGTTTGACCATATATTTAGC+GGG - chr5_4:6386565-6386584 Msa0833230:CDS 35.0%
GAAGCAGACATAATAACAAC+AGG + chr5_4:6386646-6386665 None:intergenic 35.0%
TCTATTCAACAACTTCAAGG+TGG - chr5_4:6386718-6386737 Msa0833230:CDS 35.0%
TTAGGATTAGAATTAGGAGG+AGG + chr5_4:6386835-6386854 None:intergenic 35.0%
AGATGCCTTCTGATAATTTC+TGG - chr5_4:6386878-6386897 Msa0833230:CDS 35.0%
GCTGTAAAGTGTGGAATTGA+TGG + chr5_4:6386063-6386082 None:intergenic 40.0%
!! TGTTTTCTTCTCGGTTTGTG+AGG + chr5_4:6386147-6386166 None:intergenic 40.0%
TTTAGCCCCAAGTTAGAATC+CGG - chr5_4:6386190-6386209 Msa0833230:CDS 40.0%
TCAATGACAAGAAGACCAAG+AGG - chr5_4:6386241-6386260 Msa0833230:CDS 40.0%
!!! CGGTGGTTTTGGTTTGTTTT+TGG + chr5_4:6386281-6386300 None:intergenic 40.0%
TCACGTGTGATAATAATCGG+TGG + chr5_4:6386298-6386317 None:intergenic 40.0%
! CTCCATCACATGAGTTTTCA+AGG + chr5_4:6386332-6386351 None:intergenic 40.0%
CTTGAAAACTCATGTGATGG+AGG - chr5_4:6386330-6386349 Msa0833230:CDS 40.0%
CTGTTGTTACACTTCATGGA+AGG - chr5_4:6386482-6386501 Msa0833230:CDS 40.0%
TTGACCATATATTTAGCGGG+CGG - chr5_4:6386568-6386587 Msa0833230:CDS 40.0%
TGCTTATGAGAGACTTCCTT+TGG - chr5_4:6386678-6386697 Msa0833230:CDS 40.0%
! CTTCCTTTGGAAGATGATGA+TGG - chr5_4:6386691-6386710 Msa0833230:CDS 40.0%
GAACCATCATCATCTTCCAA+AGG + chr5_4:6386697-6386716 None:intergenic 40.0%
ATTCAACAACTTCAAGGTGG+TGG - chr5_4:6386721-6386740 Msa0833230:CDS 40.0%
! CAGCAACAACAACAGCTTTT+AGG - chr5_4:6386778-6386797 Msa0833230:CDS 40.0%
GTTGGCCAGAAATTATCAGA+AGG + chr5_4:6386886-6386905 None:intergenic 40.0%
!! TCTGATAATTTCTGGCCAAC+CGG - chr5_4:6386886-6386905 Msa0833230:CDS 40.0%
GCTGTAAAGTGTGGAATTGA+TGG + chr5_4:6386063-6386082 None:intergenic 40.0%
!! TGTTTTCTTCTCGGTTTGTG+AGG + chr5_4:6386147-6386166 None:intergenic 40.0%
TTTAGCCCCAAGTTAGAATC+CGG - chr5_4:6386190-6386209 Msa0833230:CDS 40.0%
TCAATGACAAGAAGACCAAG+AGG - chr5_4:6386241-6386260 Msa0833230:CDS 40.0%
!!! CGGTGGTTTTGGTTTGTTTT+TGG + chr5_4:6386281-6386300 None:intergenic 40.0%
TCACGTGTGATAATAATCGG+TGG + chr5_4:6386298-6386317 None:intergenic 40.0%
! CTCCATCACATGAGTTTTCA+AGG + chr5_4:6386332-6386351 None:intergenic 40.0%
CTTGAAAACTCATGTGATGG+AGG - chr5_4:6386330-6386349 Msa0833230:CDS 40.0%
CTGTTGTTACACTTCATGGA+AGG - chr5_4:6386482-6386501 Msa0833230:CDS 40.0%
TTGACCATATATTTAGCGGG+CGG - chr5_4:6386568-6386587 Msa0833230:CDS 40.0%
TGCTTATGAGAGACTTCCTT+TGG - chr5_4:6386678-6386697 Msa0833230:CDS 40.0%
! CTTCCTTTGGAAGATGATGA+TGG - chr5_4:6386691-6386710 Msa0833230:CDS 40.0%
GAACCATCATCATCTTCCAA+AGG + chr5_4:6386697-6386716 None:intergenic 40.0%
ATTCAACAACTTCAAGGTGG+TGG - chr5_4:6386721-6386740 Msa0833230:CDS 40.0%
! CAGCAACAACAACAGCTTTT+AGG - chr5_4:6386778-6386797 Msa0833230:CDS 40.0%
GTTGGCCAGAAATTATCAGA+AGG + chr5_4:6386886-6386905 None:intergenic 40.0%
!! TCTGATAATTTCTGGCCAAC+CGG - chr5_4:6386886-6386905 Msa0833230:CDS 40.0%
! AGATCTCTTGCTGTGTGGAA+AGG + chr5_4:6386001-6386020 None:intergenic 45.0%
! GATGAAGATCTCTTGCTGTG+TGG + chr5_4:6386006-6386025 None:intergenic 45.0%
GTTGTTGCTGCTGTAAAGTG+TGG + chr5_4:6386072-6386091 None:intergenic 45.0%
!!! GGTTTGTTTTTGGATCCAGC+TGG + chr5_4:6386271-6386290 None:intergenic 45.0%
CGCCTTGAAAACTCATGTGA+TGG - chr5_4:6386327-6386346 Msa0833230:CDS 45.0%
TGATGCAAACGCCTCTTTGA+CGG + chr5_4:6386405-6386424 None:intergenic 45.0%
CGGTTACAAACGTGACACTA+AGG - chr5_4:6386440-6386459 Msa0833230:CDS 45.0%
AAACGTGACACTAAGGCAAC+CGG - chr5_4:6386447-6386466 Msa0833230:CDS 45.0%
TGAAGTGTAACAACAGCTCC+AGG + chr5_4:6386478-6386497 None:intergenic 45.0%
GGAGCTGTTGTTACACTTCA+TGG - chr5_4:6386478-6386497 Msa0833230:CDS 45.0%
AAGGTTCGAGATTCTTTCGC+TGG - chr5_4:6386501-6386520 Msa0833230:CDS 45.0%
TATGGTCAAACCAGAAGCAG+CGG + chr5_4:6386557-6386576 None:intergenic 45.0%
TTGTCCGCCCGCTAAATATA+TGG + chr5_4:6386575-6386594 None:intergenic 45.0%
! ATATATTTAGCGGGCGGACA+AGG - chr5_4:6386574-6386593 Msa0833230:CDS 45.0%
! TATATTTAGCGGGCGGACAA+GGG - chr5_4:6386575-6386594 Msa0833230:CDS 45.0%
!! GTTGTTGGTGGAAGTGTTGT+TGG - chr5_4:6386601-6386620 Msa0833230:CDS 45.0%
! GTTGGTGCTTTGATTGCTTC+TGG - chr5_4:6386619-6386638 Msa0833230:CDS 45.0%
! GGAGGCATAGCTTGAAAAAG+TGG + chr5_4:6386817-6386836 None:intergenic 45.0%
! AGATCTCTTGCTGTGTGGAA+AGG + chr5_4:6386001-6386020 None:intergenic 45.0%
! GATGAAGATCTCTTGCTGTG+TGG + chr5_4:6386006-6386025 None:intergenic 45.0%
GTTGTTGCTGCTGTAAAGTG+TGG + chr5_4:6386072-6386091 None:intergenic 45.0%
!!! GGTTTGTTTTTGGATCCAGC+TGG + chr5_4:6386271-6386290 None:intergenic 45.0%
CGCCTTGAAAACTCATGTGA+TGG - chr5_4:6386327-6386346 Msa0833230:CDS 45.0%
TGATGCAAACGCCTCTTTGA+CGG + chr5_4:6386405-6386424 None:intergenic 45.0%
CGGTTACAAACGTGACACTA+AGG - chr5_4:6386440-6386459 Msa0833230:CDS 45.0%
AAACGTGACACTAAGGCAAC+CGG - chr5_4:6386447-6386466 Msa0833230:CDS 45.0%
TGAAGTGTAACAACAGCTCC+AGG + chr5_4:6386478-6386497 None:intergenic 45.0%
GGAGCTGTTGTTACACTTCA+TGG - chr5_4:6386478-6386497 Msa0833230:CDS 45.0%
AAGGTTCGAGATTCTTTCGC+TGG - chr5_4:6386501-6386520 Msa0833230:CDS 45.0%
TATGGTCAAACCAGAAGCAG+CGG + chr5_4:6386557-6386576 None:intergenic 45.0%
TTGTCCGCCCGCTAAATATA+TGG + chr5_4:6386575-6386594 None:intergenic 45.0%
! ATATATTTAGCGGGCGGACA+AGG - chr5_4:6386574-6386593 Msa0833230:CDS 45.0%
! TATATTTAGCGGGCGGACAA+GGG - chr5_4:6386575-6386594 Msa0833230:CDS 45.0%
!! GTTGTTGGTGGAAGTGTTGT+TGG - chr5_4:6386601-6386620 Msa0833230:CDS 45.0%
! GTTGGTGCTTTGATTGCTTC+TGG - chr5_4:6386619-6386638 Msa0833230:CDS 45.0%
! GGAGGCATAGCTTGAAAAAG+TGG + chr5_4:6386817-6386836 None:intergenic 45.0%
! TCTCTTGCTGTGTGGAAAGG+TGG + chr5_4:6385998-6386017 None:intergenic 50.0%
!! GTTGCTGCTGTTGGTGTTGA+TGG + chr5_4:6386033-6386052 None:intergenic 50.0%
GAACAAAGCGGAAGTAGCAG+CGG - chr5_4:6386109-6386128 Msa0833230:CDS 50.0%
CTCCTCCGGATTCTAACTTG+GGG + chr5_4:6386198-6386217 None:intergenic 50.0%
CCTCCTCCGGATTCTAACTT+GGG + chr5_4:6386199-6386218 None:intergenic 50.0%
ACCTCCTCCGGATTCTAACT+TGG + chr5_4:6386200-6386219 None:intergenic 50.0%
AAGTTAGAATCCGGAGGAGG+TGG - chr5_4:6386199-6386218 Msa0833230:CDS 50.0%
! CATGTGATGGAGGTTGCAGA+CGG - chr5_4:6386340-6386359 Msa0833230:CDS 50.0%
TTTGCTAGACGCCGTCAAAG+AGG - chr5_4:6386391-6386410 Msa0833230:CDS 50.0%
!! AGAGGCGTTTGCATCATGAG+TGG - chr5_4:6386409-6386428 Msa0833230:CDS 50.0%
! GAGGCGTTTGCATCATGAGT+GGG - chr5_4:6386410-6386429 Msa0833230:CDS 50.0%
GTTTGCATCATGAGTGGGAC+AGG - chr5_4:6386415-6386434 Msa0833230:CDS 50.0%
CATCATGAGTGGGACAGGAA+CGG - chr5_4:6386420-6386439 Msa0833230:CDS 50.0%
TTCGAGATTCTTTCGCTGGC+TGG - chr5_4:6386505-6386524 Msa0833230:CDS 50.0%
ATGGTCAAACCAGAAGCAGC+GGG + chr5_4:6386556-6386575 None:intergenic 50.0%
! CAACAACTTCAAGGTGGTGG+TGG - chr5_4:6386724-6386743 Msa0833230:CDS 50.0%
GGCATCTGACCGAGAAGATT+AGG + chr5_4:6386865-6386884 None:intergenic 50.0%
! TCTCTTGCTGTGTGGAAAGG+TGG + chr5_4:6385998-6386017 None:intergenic 50.0%
!! GTTGCTGCTGTTGGTGTTGA+TGG + chr5_4:6386033-6386052 None:intergenic 50.0%
GAACAAAGCGGAAGTAGCAG+CGG - chr5_4:6386109-6386128 Msa0833230:CDS 50.0%
CTCCTCCGGATTCTAACTTG+GGG + chr5_4:6386198-6386217 None:intergenic 50.0%
CCTCCTCCGGATTCTAACTT+GGG + chr5_4:6386199-6386218 None:intergenic 50.0%
ACCTCCTCCGGATTCTAACT+TGG + chr5_4:6386200-6386219 None:intergenic 50.0%
AAGTTAGAATCCGGAGGAGG+TGG - chr5_4:6386199-6386218 Msa0833230:CDS 50.0%
! CATGTGATGGAGGTTGCAGA+CGG - chr5_4:6386340-6386359 Msa0833230:CDS 50.0%
TTTGCTAGACGCCGTCAAAG+AGG - chr5_4:6386391-6386410 Msa0833230:CDS 50.0%
!! AGAGGCGTTTGCATCATGAG+TGG - chr5_4:6386409-6386428 Msa0833230:CDS 50.0%
! GAGGCGTTTGCATCATGAGT+GGG - chr5_4:6386410-6386429 Msa0833230:CDS 50.0%
GTTTGCATCATGAGTGGGAC+AGG - chr5_4:6386415-6386434 Msa0833230:CDS 50.0%
CATCATGAGTGGGACAGGAA+CGG - chr5_4:6386420-6386439 Msa0833230:CDS 50.0%
TTCGAGATTCTTTCGCTGGC+TGG - chr5_4:6386505-6386524 Msa0833230:CDS 50.0%
ATGGTCAAACCAGAAGCAGC+GGG + chr5_4:6386556-6386575 None:intergenic 50.0%
! CAACAACTTCAAGGTGGTGG+TGG - chr5_4:6386724-6386743 Msa0833230:CDS 50.0%
GGCATCTGACCGAGAAGATT+AGG + chr5_4:6386865-6386884 None:intergenic 50.0%
! CTTGCTGTGTGGAAAGGTGG+AGG + chr5_4:6385995-6386014 None:intergenic 55.0%
!! GGTGCTGTTGTTGCTGCTGT+TGG + chr5_4:6386042-6386061 None:intergenic 55.0%
AGCCCCAAGTTAGAATCCGG+AGG - chr5_4:6386193-6386212 Msa0833230:CDS 55.0%
CCCAAGTTAGAATCCGGAGG+AGG - chr5_4:6386196-6386215 Msa0833230:CDS 55.0%
CATTGAATCCGTGTCGCCAC+CGG + chr5_4:6386227-6386246 None:intergenic 55.0%
AGACCAAGAGGAAGACCAGC+TGG - chr5_4:6386253-6386272 Msa0833230:CDS 55.0%
!! GATCCAGCTGGTCTTCCTCT+TGG + chr5_4:6386259-6386278 None:intergenic 55.0%
ACAACAGCTCCAGGAGAAGC+CGG + chr5_4:6386469-6386488 None:intergenic 55.0%
!! CAACTTCAAGGTGGTGGTGG+TGG - chr5_4:6386727-6386746 Msa0833230:CDS 55.0%
TGAACACCACCACCACCACT+TGG + chr5_4:6386760-6386779 None:intergenic 55.0%
CAATACGGAGAACGACCGGT+TGG + chr5_4:6386904-6386923 None:intergenic 55.0%
! CTTGCTGTGTGGAAAGGTGG+AGG + chr5_4:6385995-6386014 None:intergenic 55.0%
!! GGTGCTGTTGTTGCTGCTGT+TGG + chr5_4:6386042-6386061 None:intergenic 55.0%
AGCCCCAAGTTAGAATCCGG+AGG - chr5_4:6386193-6386212 Msa0833230:CDS 55.0%
CCCAAGTTAGAATCCGGAGG+AGG - chr5_4:6386196-6386215 Msa0833230:CDS 55.0%
CATTGAATCCGTGTCGCCAC+CGG + chr5_4:6386227-6386246 None:intergenic 55.0%
AGACCAAGAGGAAGACCAGC+TGG - chr5_4:6386253-6386272 Msa0833230:CDS 55.0%
!! GATCCAGCTGGTCTTCCTCT+TGG + chr5_4:6386259-6386278 None:intergenic 55.0%
ACAACAGCTCCAGGAGAAGC+CGG + chr5_4:6386469-6386488 None:intergenic 55.0%
!! CAACTTCAAGGTGGTGGTGG+TGG - chr5_4:6386727-6386746 Msa0833230:CDS 55.0%
TGAACACCACCACCACCACT+TGG + chr5_4:6386760-6386779 None:intergenic 55.0%
CAATACGGAGAACGACCGGT+TGG + chr5_4:6386904-6386923 None:intergenic 55.0%
CAAAGCGGAAGTAGCAGCGG+CGG - chr5_4:6386112-6386131 Msa0833230:CDS 60.0%
GAATCCGGAGGAGGTGGTTC+CGG - chr5_4:6386205-6386224 Msa0833230:CDS 60.0%
! CTAAGGCAACCGGCTTCTCC+TGG - chr5_4:6386457-6386476 Msa0833230:CDS 60.0%
GTCAAACCAGAAGCAGCGGG+CGG + chr5_4:6386553-6386572 None:intergenic 60.0%
GGACAAGGGCAGGTTGTTGG+TGG - chr5_4:6386589-6386608 Msa0833230:CDS 60.0%
!! CTTCAAGGTGGTGGTGGTGG+TGG - chr5_4:6386730-6386749 Msa0833230:CDS 60.0%
!! GGTGGTGGTGGTTCTCCAAG+TGG - chr5_4:6386742-6386761 Msa0833230:CDS 60.0%
! GGTGGTGGTTCTCCAAGTGG+TGG - chr5_4:6386745-6386764 Msa0833230:CDS 60.0%
GGTGGTTCTCCAAGTGGTGG+TGG - chr5_4:6386748-6386767 Msa0833230:CDS 60.0%
!! GGTTCTCCAAGTGGTGGTGG+TGG - chr5_4:6386751-6386770 Msa0833230:CDS 60.0%
CAAAGCGGAAGTAGCAGCGG+CGG - chr5_4:6386112-6386131 Msa0833230:CDS 60.0%
GAATCCGGAGGAGGTGGTTC+CGG - chr5_4:6386205-6386224 Msa0833230:CDS 60.0%
! CTAAGGCAACCGGCTTCTCC+TGG - chr5_4:6386457-6386476 Msa0833230:CDS 60.0%
GTCAAACCAGAAGCAGCGGG+CGG + chr5_4:6386553-6386572 None:intergenic 60.0%
GGACAAGGGCAGGTTGTTGG+TGG - chr5_4:6386589-6386608 Msa0833230:CDS 60.0%
!! CTTCAAGGTGGTGGTGGTGG+TGG - chr5_4:6386730-6386749 Msa0833230:CDS 60.0%
!! GGTGGTGGTGGTTCTCCAAG+TGG - chr5_4:6386742-6386761 Msa0833230:CDS 60.0%
! GGTGGTGGTTCTCCAAGTGG+TGG - chr5_4:6386745-6386764 Msa0833230:CDS 60.0%
GGTGGTTCTCCAAGTGGTGG+TGG - chr5_4:6386748-6386767 Msa0833230:CDS 60.0%
!! GGTTCTCCAAGTGGTGGTGG+TGG - chr5_4:6386751-6386770 Msa0833230:CDS 60.0%
AGGTGGTTCCGGTGGCGACA+CGG - chr5_4:6386216-6386235 Msa0833230:CDS 65.0%
! TTTAGCGGGCGGACAAGGGC+AGG - chr5_4:6386579-6386598 Msa0833230:CDS 65.0%
GGCGGACAAGGGCAGGTTGT+TGG - chr5_4:6386586-6386605 Msa0833230:CDS 65.0%
AGGTGGTTCCGGTGGCGACA+CGG - chr5_4:6386216-6386235 Msa0833230:CDS 65.0%
! TTTAGCGGGCGGACAAGGGC+AGG - chr5_4:6386579-6386598 Msa0833230:CDS 65.0%
GGCGGACAAGGGCAGGTTGT+TGG - chr5_4:6386586-6386605 Msa0833230:CDS 65.0%
AGCGGAAGTAGCAGCGGCGG+TGG - chr5_4:6386115-6386134 Msa0833230:CDS 70.0%
TCCGGAGGAGGTGGTTCCGG+TGG - chr5_4:6386208-6386227 Msa0833230:CDS 70.0%
GCCACCGGAACCACCTCCTC+CGG + chr5_4:6386212-6386231 None:intergenic 70.0%
AGCGGAAGTAGCAGCGGCGG+TGG - chr5_4:6386115-6386134 Msa0833230:CDS 70.0%
TCCGGAGGAGGTGGTTCCGG+TGG - chr5_4:6386208-6386227 Msa0833230:CDS 70.0%
GCCACCGGAACCACCTCCTC+CGG + chr5_4:6386212-6386231 None:intergenic 70.0%
GAAGCAGCGGGCGGAGCAGG+TGG + chr5_4:6386544-6386563 None:intergenic 75.0%
CCAGAAGCAGCGGGCGGAGC+AGG + chr5_4:6386547-6386566 None:intergenic 75.0%
CCTGCTCCGCCCGCTGCTTC+TGG - chr5_4:6386544-6386563 Msa0833230:CDS 75.0%
GAAGCAGCGGGCGGAGCAGG+TGG + chr5_4:6386544-6386563 None:intergenic 75.0%
CCAGAAGCAGCGGGCGGAGC+AGG + chr5_4:6386547-6386566 None:intergenic 75.0%
CCTGCTCCGCCCGCTGCTTC+TGG - chr5_4:6386544-6386563 Msa0833230:CDS 75.0%
! GCAGCGGGCGGAGCAGGTGG+TGG + chr5_4:6386541-6386560 None:intergenic 80.0%
! GCAGCGGGCGGAGCAGGTGG+TGG + chr5_4:6386541-6386560 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
chr5_4 gene 6385959 6386924 6385959 ID=Msa0833230;Name=Msa0833230
chr5_4 mRNA 6385959 6386924 6385959 ID=Msa0833230-mRNA-1;Parent=Msa0833230;Name=Msa0833230-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|321
chr5_4 exon 6385959 6386924 6385959 ID=Msa0833230-mRNA-1:exon:2764;Parent=Msa0833230-mRNA-1
chr5_4 CDS 6385959 6386924 6385959 ID=Msa0833230-mRNA-1:cds;Parent=Msa0833230-mRNA-1
Gene Sequence

>Msa0833230

ATGGATCAAATAACATCACATGGACATTCACTTCCTCCACCTTTCCACACAGCAAGAGATCTTCATCTTCACCATCAACACCAACAGCAGCAACAACAGCACCATCAATTCCACACTTTACAGCAGCAACAACAAACAACAGATCAAGATGAACAAAGCGGAAGTAGCAGCGGCGGTGGACTCAACCTCACAAACCGAGAAGAAAACAGCAACAACAAGTTCAGCACAGAATTTAGCCCCAAGTTAGAATCCGGAGGAGGTGGTTCCGGTGGCGACACGGATTCAATGACAAGAAGACCAAGAGGAAGACCAGCTGGATCCAAAAACAAACCAAAACCACCGATTATTATCACACGTGACAGCGCAAACGCCTTGAAAACTCATGTGATGGAGGTTGCAGACGGTTGCGACGTTGTTGAAAGTGTCAATAACTTTGCTAGACGCCGTCAAAGAGGCGTTTGCATCATGAGTGGGACAGGAACGGTTACAAACGTGACACTAAGGCAACCGGCTTCTCCTGGAGCTGTTGTTACACTTCATGGAAGGTTCGAGATTCTTTCGCTGGCTGGATCGTTTCTTCCACCACCTGCTCCGCCCGCTGCTTCTGGTTTGACCATATATTTAGCGGGCGGACAAGGGCAGGTTGTTGGTGGAAGTGTTGTTGGTGCTTTGATTGCTTCTGGACCTGTTGTTATTATGTCTGCTTCTTTTAGCAACGCTGCTTATGAGAGACTTCCTTTGGAAGATGATGATGGTTCTTCTATTCAACAACTTCAAGGTGGTGGTGGTGGTGGTTCTCCAAGTGGTGGTGGTGGTGTTCAGCAACAACAACAGCTTTTAGGAGATTCAACTGCTCCACTTTTTCAAGCTATGCCTCCTCCTAATTCTAATCCTAATCTTAATCCTAATCTTCTCGGTCAGATGCCTTCTGATAATTTCTGGCCAACCGGTCGTTCTCCGTATTGA
Protein sequence

>Msa0833230

MDQITSHGHSLPPPFHTARDLHLHHQHQQQQQQHHQFHTLQQQQQTTDQDEQSGSSSGGGLNLTNREENSNNKFSTEFSPKLESGGGGSGGDTDSMTRRPRGRPAGSKNKPKPPIIITRDSANALKTHVMEVADGCDVVESVNNFARRRQRGVCIMSGTGTVTNVTLRQPASPGAVVTLHGRFEILSLAGSFLPPPAPPAASGLTIYLAGGQGQVVGGSVVGALIASGPVVIMSASFSNAAYERLPLEDDDGSSIQQLQGGGGGGSPSGGGGVQQQQQLLGDSTAPLFQAMPPPNSNPNLNPNLLGQMPSDNFWPTGRSPY*