Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0491760 | XP_003598648.1 | 97.452 | 314 | 8 | 0 | 1 | 314 | 1 | 314 | 0.0 | 627 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0491760 | sp|P51074|ANX4_FRAAN | 65.924 | 314 | 107 | 0 | 1 | 314 | 1 | 314 | 3.87e-155 | 439 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0004580 | Msa0491760 | 0.826100 | 3.264930e-54 | -8.615850e-47 |
| Msa0004590 | Msa0491760 | 0.850161 | 2.031238e-60 | -8.615850e-47 |
| Msa0020320 | Msa0491760 | -0.801506 | 8.719647e-49 | -8.615850e-47 |
| Msa0024280 | Msa0491760 | 0.810085 | 1.373385e-50 | -8.615850e-47 |
| Msa0027860 | Msa0491760 | 0.800672 | 1.291058e-48 | -8.615850e-47 |
| Msa0032130 | Msa0491760 | 0.801645 | 8.165397e-49 | -8.615850e-47 |
| Msa0036580 | Msa0491760 | 0.820400 | 6.996658e-53 | -8.615850e-47 |
| Msa0037500 | Msa0491760 | 0.806351 | 8.581600e-50 | -8.615850e-47 |
| Msa0045620 | Msa0491760 | 0.805958 | 1.038049e-49 | -8.615850e-47 |
| Msa0048950 | Msa0491760 | 0.859504 | 3.952396e-63 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0491760 | MtrunA17_Chr3g0083901 | 97.452 | 314 | 8 | 0 | 1 | 314 | 1 | 314 | 0.0 | 627 |
| Msa0491760 | MtrunA17_Chr8g0352621 | 78.344 | 314 | 67 | 1 | 1 | 314 | 1 | 313 | 0.0 | 524 |
| Msa0491760 | MtrunA17_Chr8g0352611 | 71.975 | 314 | 87 | 1 | 1 | 314 | 1 | 313 | 6.74e-166 | 463 |
| Msa0491760 | MtrunA17_Chr3g0083921 | 67.834 | 314 | 101 | 0 | 1 | 314 | 1 | 314 | 3.98e-161 | 451 |
| Msa0491760 | MtrunA17_Chr8g0352591 | 70.192 | 312 | 82 | 1 | 1 | 312 | 1 | 301 | 9.25e-161 | 450 |
| Msa0491760 | MtrunA17_Chr2g0293441 | 47.588 | 311 | 163 | 0 | 1 | 311 | 1 | 311 | 2.39e-109 | 320 |
| Msa0491760 | MtrunA17_Chr5g0425971 | 46.349 | 315 | 168 | 1 | 1 | 314 | 1 | 315 | 3.40e-99 | 294 |
| Msa0491760 | MtrunA17_Chr8g0382281 | 47.604 | 313 | 163 | 1 | 1 | 312 | 1 | 313 | 4.00e-97 | 289 |
| Msa0491760 | MtrunA17_Chr8g0352571 | 40.984 | 305 | 177 | 1 | 11 | 312 | 12 | 316 | 1.06e-75 | 234 |
| Msa0491760 | MtrunA17_Chr8g0393281 | 38.535 | 314 | 190 | 3 | 1 | 312 | 1 | 313 | 2.04e-71 | 223 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0491760 | AT5G12380.1 | 53.376 | 311 | 145 | 0 | 1 | 311 | 1 | 311 | 2.80e-122 | 353 |
| Msa0491760 | AT1G35720.1 | 50.633 | 316 | 154 | 2 | 1 | 314 | 1 | 316 | 4.25e-109 | 320 |
| Msa0491760 | AT5G10230.1 | 49.206 | 315 | 158 | 2 | 1 | 314 | 1 | 314 | 8.08e-104 | 306 |
| Msa0491760 | AT5G10220.1 | 48.896 | 317 | 158 | 3 | 1 | 314 | 1 | 316 | 2.89e-103 | 305 |
| Msa0491760 | AT5G65020.1 | 49.051 | 316 | 159 | 2 | 1 | 314 | 1 | 316 | 4.33e-103 | 305 |
| Msa0491760 | AT5G65020.2 | 49.485 | 291 | 146 | 1 | 25 | 314 | 11 | 301 | 1.86e-97 | 290 |
| Msa0491760 | AT2G38760.1 | 41.195 | 318 | 180 | 3 | 1 | 311 | 1 | 318 | 3.62e-81 | 249 |
| Msa0491760 | AT1G68090.1 | 36.508 | 315 | 198 | 2 | 1 | 313 | 1 | 315 | 1.10e-65 | 209 |
| Msa0491760 | AT2G38750.2 | 35.390 | 308 | 174 | 7 | 25 | 314 | 2 | 302 | 3.07e-49 | 166 |
| Msa0491760 | AT2G38750.1 | 35.390 | 308 | 174 | 7 | 25 | 314 | 19 | 319 | 3.89e-49 | 166 |
Find 65 sgRNAs with CRISPR-Local
Find 225 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAACAGCATCACGGTCTGC+AGG | 0.524079 | 3_4:-14704018 | None:intergenic |
| AAATTTGGAACCGATGAAGA+TGG | 0.484865 | 3_4:+14705367 | Msa0491760:CDS |
| AACCGTTATCGAGATGAACA+TGG | 0.608246 | 3_4:+14704615 | Msa0491760:CDS |
| AAGGAGCAAGACTCAACTTG+TGG | 0.646109 | 3_4:+14704584 | Msa0491760:CDS |
| AAGGGCTGAAAAGGATCTGA+AGG | 0.556711 | 3_4:+14705411 | Msa0491760:CDS |
| AAGTTGAGTCTTGCTCCTTG+TGG | 0.582635 | 3_4:-14704580 | None:intergenic |
| AATTTGGAACCGATGAAGAT+GGG | 0.559458 | 3_4:+14705368 | Msa0491760:CDS |
| ACAGATTAGAAAAGCTTATG+AGG | 0.471180 | 3_4:+14703723 | Msa0491760:CDS |
| ACATGGCATCTCCATTAGTA+AGG | 0.533720 | 3_4:+14704632 | Msa0491760:CDS |
| ACGCTTGTAGCGGTTATGAT+AGG | 0.478212 | 3_4:-14704139 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| CGTAAAATAAGTTCTCATTG+AGG | - | chr3_4:14702279-14702298 | None:intergenic | 30.0% | |
| ATGAATAATCAATACCACCA+AGG | - | chr3_4:14702605-14702624 | None:intergenic | 30.0% | |
| CCTACTAAATACCATTTATG+GGG | + | chr3_4:14702659-14702678 | Msa0491760:intron | 30.0% | |
| AAATACCATTTATGGGGATA+AGG | + | chr3_4:14702665-14702684 | Msa0491760:intron | 30.0% | |
| AATACCATTTATGGGGATAA+GGG | + | chr3_4:14702666-14702685 | Msa0491760:intron | 30.0% | |
| TGTGCTTCAATATAAAGTGT+CGG | + | chr3_4:14703181-14703200 | Msa0491760:intron | 30.0% | |
| CTTATGATTGATCAATGTGA+TGG | + | chr3_4:14703211-14703230 | Msa0491760:intron | 30.0% | |
| ACAGATTAGAAAAGCTTATG+AGG | + | chr3_4:14703723-14703742 | Msa0491760:CDS | 30.0% | |
| TTGACCACATAAACATCATT+TGG | - | chr3_4:14704234-14704253 | None:intergenic | 30.0% | |
| AACATAAAACTCAGTCGAAT+CGG | + | chr3_4:14704259-14704278 | Msa0491760:intron | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3_4 | gene | 14701536 | 14705549 | 14701536 | ID=Msa0491760;Name=Msa0491760 |
| chr3_4 | mRNA | 14701536 | 14705549 | 14701536 | ID=Msa0491760-mRNA-1;Parent=Msa0491760;Name=Msa0491760-mRNA-1;_AED=0.17;_eAED=0.17;_QI=51|1|0.83|1|1|1|6|0|314 |
| chr3_4 | exon | 14701536 | 14701659 | 14701536 | ID=Msa0491760-mRNA-1:exon:4340;Parent=Msa0491760-mRNA-1 |
| chr3_4 | exon | 14703659 | 14703804 | 14703659 | ID=Msa0491760-mRNA-1:exon:4341;Parent=Msa0491760-mRNA-1 |
| chr3_4 | exon | 14703991 | 14704209 | 14703991 | ID=Msa0491760-mRNA-1:exon:4342;Parent=Msa0491760-mRNA-1 |
| chr3_4 | exon | 14704441 | 14704653 | 14704441 | ID=Msa0491760-mRNA-1:exon:4343;Parent=Msa0491760-mRNA-1 |
| chr3_4 | exon | 14704982 | 14705071 | 14704982 | ID=Msa0491760-mRNA-1:exon:4344;Parent=Msa0491760-mRNA-1 |
| chr3_4 | exon | 14705346 | 14705549 | 14705346 | ID=Msa0491760-mRNA-1:exon:4345;Parent=Msa0491760-mRNA-1 |
| chr3_4 | five_prime_UTR | 14701536 | 14701586 | 14701536 | ID=Msa0491760-mRNA-1:five_prime_utr;Parent=Msa0491760-mRNA-1 |
| chr3_4 | CDS | 14701587 | 14701659 | 14701587 | ID=Msa0491760-mRNA-1:cds;Parent=Msa0491760-mRNA-1 |
| chr3_4 | CDS | 14703659 | 14703804 | 14703659 | ID=Msa0491760-mRNA-1:cds;Parent=Msa0491760-mRNA-1 |
| chr3_4 | CDS | 14703991 | 14704209 | 14703991 | ID=Msa0491760-mRNA-1:cds;Parent=Msa0491760-mRNA-1 |
| chr3_4 | CDS | 14704441 | 14704653 | 14704441 | ID=Msa0491760-mRNA-1:cds;Parent=Msa0491760-mRNA-1 |
| chr3_4 | CDS | 14704982 | 14705071 | 14704982 | ID=Msa0491760-mRNA-1:cds;Parent=Msa0491760-mRNA-1 |
| chr3_4 | CDS | 14705346 | 14705549 | 14705346 | ID=Msa0491760-mRNA-1:cds;Parent=Msa0491760-mRNA-1 |
| Gene Sequence |
>Msa0491760 ATGGCCACCATTGTTGTTCATAGCCAAACATCTCCTGTTCAAGATGCTGAGGCTCTCCGACTTGCTTTCAAAGGATGGGGTGCTGATAACAAGGCCATTATCGCAATTTTGGGTCATAGAAATGTTCATCAGAGGCAACAGATTAGAAAAGCTTATGAGGAGCTTTTCGAAGAGGATCTCATCAAACGTCTTGAGTCAGAAATCTCTGGTGACTTTGAGAGGGCTGTGTACCGGTGGATGTTGGAACCTGCAGACCGTGATGCTGTTTTGATCAATGTAGCAATCAGGAATGGCAACAAAGACTATCATGTGGTTGCTGAAATTGCTTCTGTGCTATCAACTGAAGAGCTCTTGGCAGTGAGGCGCGCCTATCATAACCGCTACAAGCGTTCCATCGAAGAAGATGCGTCTGCTCACACCACCGGTCATCTTCGCCAGCTTTTGGTTGGATTAGTGAGCTCATTTAGGTATGAGGGAGATGAGATCAATCCAAAATTGGCACAAACTGAGGCTAATATTATTCATGAAAGTGTCAAGGAAAAGAAAGGCAACAATGAAGAAGTCGTTAGGATTCTTACCACAAGGAGCAAGACTCAACTTGTGGCAACTTTCAACCGTTATCGAGATGAACATGGCATCTCCATTAGTAAGAAATTGCTGGATCAAACATCTGATGACTTCCAAAAGACATTGCACACAGCTATTCGTTGCATCAATGACCACAAAAAGTACTATGAGAAGGTTTTGCGCAATGCGATCAAGAAATTTGGAACCGATGAAGATGGGCTCAGCCGCGTGATTGTGACAAGGGCTGAAAAGGATCTGAAGGAAATCAAAGAGCTGTACTACAAGAGAAACAGTGTTCATCTTGAAGATGCAGTTTCTGAGGAAACCTCAGGGGACTACAAGAAGTTCATCCTCACTCTTTTGGGGAAACATGATTAA |
| Protein sequence |
>Msa0491760 MATIVVHSQTSPVQDAEALRLAFKGWGADNKAIIAILGHRNVHQRQQIRKAYEELFEEDLIKRLESEISGDFERAVYRWMLEPADRDAVLINVAIRNGNKDYHVVAEIASVLSTEELLAVRRAYHNRYKRSIEEDASAHTTGHLRQLLVGLVSSFRYEGDEINPKLAQTEANIIHESVKEKKGNNEEVVRILTTRSKTQLVATFNRYRDEHGISISKKLLDQTSDDFQKTLHTAIRCINDHKKYYEKVLRNAIKKFGTDEDGLSRVIVTRAEKDLKEIKELYYKRNSVHLEDAVSEETSGDYKKFILTLLGKHD* |
