AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0189680

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0189680 MtrunA17_Chr2g0278671 92.182 307 13 1 17 312 17 323 0.0 578
Msa0189680 MtrunA17_Chr4g0063671 48.276 319 148 5 6 312 7 320 2.02e-95 285
Msa0189680 MtrunA17_Chr2g0279041 48.287 321 149 6 5 312 9 325 3.59e-95 284
Msa0189680 MtrunA17_Chr4g0070951 39.634 328 171 7 3 312 11 329 8.07e-72 225
Msa0189680 MtrunA17_Chr5g0402571 39.385 325 173 7 4 312 10 326 1.37e-71 224
Msa0189680 MtrunA17_Chr2g0279061 46.693 257 123 4 68 312 12 266 6.23e-71 220
Msa0189680 MtrunA17_Chr4g0043391 41.994 331 163 11 1 312 4 324 1.71e-70 221
Msa0189680 MtrunA17_Chr5g0402581 39.077 325 174 7 4 312 10 326 1.85e-70 221
Msa0189680 MtrunA17_Chr1g0175561 39.385 325 171 10 8 312 8 326 3.52e-70 221
Msa0189680 MtrunA17_Chr2g0322861 39.252 321 170 7 10 312 73 386 4.05e-70 222
Msa0189680 MtrunA17_Chr4g0038171 41.390 331 165 11 1 312 1 321 5.60e-70 220
Msa0189680 MtrunA17_Chr7g0258811 40.122 329 170 10 3 312 51 371 6.66e-70 221
Msa0189680 MtrunA17_Chr2g0292271 40.000 330 175 8 1 312 6 330 1.07e-68 218
Msa0189680 MtrunA17_Chr2g0292211 39.752 322 171 7 9 312 25 341 1.92e-68 217
Msa0189680 MtrunA17_Chr4g0038151 40.483 331 168 10 1 312 1 321 4.79e-68 215
Msa0189680 MtrunA17_Chr2g0292181 38.138 333 179 8 2 312 12 339 1.75e-67 215
Msa0189680 MtrunA17_Chr2g0292151 39.550 311 165 8 20 312 25 330 3.94e-67 214
Msa0189680 MtrunA17_Chr4g0038161 40.303 330 169 9 1 312 1 320 5.06e-67 212
Msa0189680 MtrunA17_Chr2g0292251 40.514 311 162 8 20 312 24 329 2.37e-66 212
Msa0189680 MtrunA17_Chr1g0157051 35.714 322 185 5 1 308 1 314 6.09e-66 209
Msa0189680 MtrunA17_Chr2g0292111 37.237 333 182 9 2 312 3 330 1.62e-65 209
Msa0189680 MtrunA17_Chr2g0291751 38.941 321 169 9 12 312 17 330 2.27e-65 209
Msa0189680 MtrunA17_Chr2g0292261 40.514 311 162 8 20 312 25 330 8.82e-65 207
Msa0189680 MtrunA17_Chr2g0299691 39.077 325 176 7 6 312 38 358 1.17e-64 207
Msa0189680 MtrunA17_Chr7g0244451 38.871 319 166 8 11 312 10 316 1.83e-64 206
Msa0189680 MtrunA17_Chr7g0254231 39.604 303 156 7 24 312 22 311 3.05e-64 205
Msa0189680 MtrunA17_Chr4g0024121 37.654 324 180 7 6 312 8 326 8.23e-64 204
Msa0189680 MtrunA17_Chr2g0292241 39.228 311 166 7 20 312 25 330 1.15e-63 205
Msa0189680 MtrunA17_Chr4g0068621 38.710 310 166 8 20 312 34 336 1.28e-63 204
Msa0189680 MtrunA17_Chr2g0292091 38.253 332 178 10 1 312 1 325 3.43e-63 203
Msa0189680 MtrunA17_Chr5g0404721 40.196 306 161 10 24 312 30 330 6.79e-63 202
Msa0189680 MtrunA17_Chr7g0244461 36.533 323 176 7 5 312 4 312 2.88e-62 200
Msa0189680 MtrunA17_Chr3g0129051 38.291 316 167 8 10 308 14 318 4.26e-62 199
Msa0189680 MtrunA17_Chr3g0106921 38.650 326 168 9 6 312 8 320 3.61e-61 197
Msa0189680 MtrunA17_Chr5g0432441 39.755 327 166 9 5 312 12 326 3.71e-61 197
Msa0189680 MtrunA17_Chr7g0268501 38.110 328 177 8 1 312 3 320 6.89e-61 196
Msa0189680 MtrunA17_Chr7g0268521 38.110 328 177 8 1 312 3 320 6.89e-61 196
Msa0189680 MtrunA17_Chr6g0460711 37.658 316 178 7 9 312 6 314 8.76e-61 196
Msa0189680 MtrunA17_Chr7g0254201 36.513 304 171 6 22 312 18 312 5.37e-60 194
Msa0189680 MtrunA17_Chr3g0128061 37.963 324 176 10 8 312 9 326 6.25e-60 194
Msa0189680 MtrunA17_Chr4g0013191 39.048 315 163 10 16 312 37 340 6.93e-60 194
Msa0189680 MtrunA17_Chr4g0013171 36.747 332 179 8 1 312 1 321 2.01e-59 192
Msa0189680 MtrunA17_Chr6g0477071 37.086 302 164 8 24 312 54 342 3.52e-59 192
Msa0189680 MtrunA17_Chr5g0438021 36.607 336 178 10 1 312 1 325 3.59e-59 192
Msa0189680 MtrunA17_Chr2g0292291 35.928 334 186 10 2 312 4 332 4.38e-59 193
Msa0189680 MtrunA17_Chr7g0225221 37.152 323 179 7 5 312 7 320 7.80e-59 191
Msa0189680 MtrunA17_Chr8g0372601 37.459 307 172 6 22 312 28 330 8.02e-59 191
Msa0189680 MtrunA17_Chr8g0371381 35.974 303 171 5 24 312 26 319 1.09e-58 191
Msa0189680 MtrunA17_Chr1g0148021 34.242 330 194 6 1 312 4 328 1.22e-58 191
Msa0189680 MtrunA17_Chr1g0204011 34.483 319 186 7 9 312 12 322 1.64e-58 190
Msa0189680 MtrunA17_Chr1g0194151 36.145 332 182 8 6 312 7 333 2.44e-58 190
Msa0189680 MtrunA17_Chr2g0329641 38.907 311 163 8 21 312 33 335 9.47e-58 189
Msa0189680 MtrunA17_Chr2g0296621 37.086 302 167 7 24 312 50 341 1.52e-57 189
Msa0189680 MtrunA17_Chr2g0320071 35.474 327 182 7 1 312 5 317 1.62e-57 187
Msa0189680 MtrunA17_Chr3g0129061 37.658 316 169 9 10 308 14 318 2.20e-57 187
Msa0189680 MtrunA17_Chr2g0292101 36.280 328 182 9 5 312 6 326 2.77e-57 188
Msa0189680 MtrunA17_Chr3g0114451 36.391 327 175 7 8 312 3 318 5.53e-57 186
Msa0189680 MtrunA17_Chr2g0320101 35.366 328 185 7 1 312 1 317 2.63e-56 184
Msa0189680 MtrunA17_Chr2g0320051 36.923 325 175 9 4 312 8 318 2.82e-56 184
Msa0189680 MtrunA17_Chr4g0013181 37.086 302 162 7 28 312 63 353 6.28e-56 184
Msa0189680 MtrunA17_Chr2g0320091 35.562 329 184 8 1 312 1 318 2.31e-55 182
Msa0189680 MtrunA17_Chr5g0405991 35.061 328 185 9 1 312 1 316 3.29e-55 182
Msa0189680 MtrunA17_Chr2g0320061 36.890 328 171 12 4 312 8 318 8.36e-55 181
Msa0189680 MtrunA17_Chr5g0401431 35.780 327 183 9 4 309 6 326 8.91e-55 181
Msa0189680 MtrunA17_Chr4g0076211 36.000 300 170 6 26 312 43 333 1.43e-54 181
Msa0189680 MtrunA17_Chr3g0136751 34.862 327 187 7 2 312 6 322 3.82e-54 179
Msa0189680 MtrunA17_Chr1g0188431 35.185 324 176 9 10 312 17 327 4.01e-54 179
Msa0189680 MtrunA17_Chr2g0316191 34.030 335 170 8 22 309 74 404 1.72e-53 180
Msa0189680 MtrunA17_Chr1g0213061 34.462 325 183 8 6 312 9 321 2.13e-53 177
Msa0189680 MtrunA17_Chr2g0316791 34.132 334 170 9 22 309 74 403 3.42e-53 179
Msa0189680 MtrunA17_Chr1g0197411 36.308 325 181 9 9 312 11 330 3.67e-53 177
Msa0189680 MtrunA17_Chr4g0072091 37.582 306 170 8 23 312 23 323 4.09e-53 176
Msa0189680 MtrunA17_Chr2g0316831 34.030 335 169 9 22 309 110 439 4.52e-53 179
Msa0189680 MtrunA17_Chr1g0194271 34.218 339 186 8 6 312 7 340 5.41e-53 177
Msa0189680 MtrunA17_Chr3g0106901 37.273 330 178 7 1 312 5 323 1.37e-52 175
Msa0189680 MtrunA17_Chr4g0013201 35.294 306 172 7 22 312 44 338 1.61e-52 175
Msa0189680 MtrunA17_Chr7g0224371 35.802 324 178 9 6 312 12 322 3.25e-52 174
Msa0189680 MtrunA17_Chr2g0320031 34.056 323 186 7 6 312 8 319 9.50e-52 173
Msa0189680 MtrunA17_Chr2g0316481 32.934 334 174 8 22 309 74 403 1.08e-51 175
Msa0189680 MtrunA17_Chr3g0129041 36.875 320 174 8 10 312 14 322 1.63e-51 172
Msa0189680 MtrunA17_Chr1g0164891 35.714 308 173 8 25 312 90 392 4.19e-51 173
Msa0189680 MtrunA17_Chr5g0400331 37.304 319 170 9 17 312 20 331 8.51e-51 171
Msa0189680 MtrunA17_Chr4g0013721 36.218 312 175 8 17 312 93 396 1.40e-50 172
Msa0189680 MtrunA17_Chr4g0053871 34.756 328 182 9 5 311 7 323 2.82e-50 169
Msa0189680 MtrunA17_Chr4g0013821 35.275 309 176 9 20 312 24 324 1.16e-48 166
Msa0189680 MtrunA17_Chr4g0046131 35.294 340 181 9 4 309 9 343 1.22e-48 166
Msa0189680 MtrunA17_Chr2g0311251 32.335 334 175 8 22 309 9 337 3.89e-48 164
Msa0189680 MtrunA17_Chr3g0107881 34.848 330 181 10 3 312 9 324 5.60e-48 163
Msa0189680 MtrunA17_Chr1g0155611 36.513 304 168 8 28 312 50 347 6.64e-47 161
Msa0189680 MtrunA17_Chr8g0364291 33.832 334 184 11 5 312 2 324 3.00e-46 159
Msa0189680 MtrunA17_Chr5g0432331 33.750 320 180 10 13 312 45 352 1.06e-45 158
Msa0189680 MtrunA17_Chr5g0421411 35.495 293 151 9 47 312 4 285 3.30e-45 155
Msa0189680 MtrunA17_Chr5g0432351 32.934 334 182 11 4 312 5 321 3.31e-45 156
Msa0189680 MtrunA17_Chr2g0311241 29.412 374 174 7 22 309 58 427 6.08e-45 158
Msa0189680 MtrunA17_Chr5g0432341 33.133 332 180 11 6 312 7 321 2.88e-44 153
Msa0189680 MtrunA17_Chr6g0451571 32.615 325 189 9 9 312 19 334 2.71e-43 151
Msa0189680 MtrunA17_Chr2g0316811 36.325 234 113 6 22 221 48 279 2.14e-42 149
Msa0189680 MtrunA17_Chr4g0075241 32.484 314 176 10 22 312 27 327 6.98e-42 147
Msa0189680 MtrunA17_Chr6g0467251 32.121 330 191 7 5 312 10 328 1.76e-41 147
Msa0189680 MtrunA17_Chr5g0432401 33.654 312 155 11 9 308 11 282 3.34e-41 144
Msa0189680 MtrunA17_Chr1g0181511 31.210 314 180 8 21 312 24 323 4.65e-41 145
Msa0189680 MtrunA17_Chr6g0467161 31.683 303 182 7 24 312 45 336 7.95e-40 142
Msa0189680 MtrunA17_Chr4g0074851 29.607 331 202 7 4 312 7 328 9.58e-40 142
Msa0189680 MtrunA17_Chr5g0413051 31.529 314 187 9 21 312 70 377 7.15e-39 140
Msa0189680 MtrunA17_Chr6g0467121 29.518 332 200 9 3 312 44 363 2.38e-37 137
Msa0189680 MtrunA17_Chr1g0181481 30.255 314 183 7 21 312 24 323 8.53e-37 134
Msa0189680 MtrunA17_Chr1g0181431 30.032 313 183 7 22 312 25 323 2.43e-36 132
Msa0189680 MtrunA17_Chr3g0114491 50.435 115 53 3 18 129 13 126 9.33e-30 110
Msa0189680 MtrunA17_Chr4g0004591 27.273 319 157 7 24 309 77 353 3.00e-29 114
Msa0189680 MtrunA17_Chr2g0292281 35.211 213 112 7 113 312 17 216 4.82e-28 108
Msa0189680 MtrunA17_Chr2g0316141 29.710 276 147 8 70 309 11 275 2.50e-27 107
Msa0189680 MtrunA17_Chr2g0316181 29.710 276 147 8 70 309 11 275 2.50e-27 107
Msa0189680 MtrunA17_Chr6g0468731 61.765 68 25 1 63 130 22 88 1.59e-23 92.4
Msa0189680 MtrunA17_Chr1g0181491 41.964 112 62 2 21 130 9 119 2.71e-21 88.2
Msa0189680 MtrunA17_Chr7g0254181 44.681 94 50 1 84 177 22 113 1.66e-19 83.6
Msa0189680 MtrunA17_Chr3g0114461 29.146 199 117 5 127 312 5 192 1.12e-14 71.2
Msa0189680 MtrunA17_Chr7g0254171 43.678 87 46 2 70 153 4 90 1.61e-14 69.7
Msa0189680 MtrunA17_Chr1g0196191 50.000 74 33 2 57 128 20 91 8.29e-14 68.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0189680 AT4G25980.1 62.893 318 103 3 7 312 12 326 1.34e-140 400
Msa0189680 AT1G77100.1 52.960 321 131 5 5 312 6 319 1.35e-105 311
Msa0189680 AT2G41480.1 46.769 325 159 4 1 312 5 328 1.81e-96 288
Msa0189680 AT5G64120.1 45.574 305 149 4 19 312 30 328 1.35e-87 265
Msa0189680 AT5G39580.1 42.991 321 163 4 4 312 7 319 2.72e-84 257
Msa0189680 AT5G64100.1 43.243 333 159 8 4 312 5 331 1.33e-78 243
Msa0189680 AT5G17820.1 43.260 319 157 8 6 312 7 313 8.21e-77 238
Msa0189680 AT5G17820.2 43.260 319 157 8 6 312 60 366 4.99e-76 237
Msa0189680 AT1G05260.1 41.486 323 170 7 5 312 7 325 1.77e-75 234
Msa0189680 AT5G64110.1 41.975 324 162 7 9 312 13 330 2.78e-75 234
Msa0189680 AT5G15180.1 41.590 327 171 8 1 312 8 329 4.36e-74 231
Msa0189680 AT3G01190.1 41.745 321 168 6 5 312 7 321 3.12e-73 228
Msa0189680 AT4G11290.1 41.538 325 167 7 7 312 6 326 4.26e-72 226
Msa0189680 AT4G25980.2 68.182 154 49 0 20 173 67 220 2.06e-71 223
Msa0189680 AT1G05240.1 40.728 302 158 7 24 309 26 322 3.29e-70 221
Msa0189680 AT1G05250.1 40.728 302 158 7 24 309 26 322 3.29e-70 221
Msa0189680 AT3G21770.1 39.806 309 167 6 16 309 21 325 6.56e-70 220
Msa0189680 AT1G49570.1 42.395 309 154 9 19 308 43 346 1.07e-68 218
Msa0189680 AT4G36430.1 39.441 322 172 9 9 312 14 330 6.75e-66 210
Msa0189680 AT4G26010.1 38.679 318 168 6 8 312 6 309 1.25e-65 209
Msa0189680 AT4G26010.2 38.679 318 168 6 8 312 34 337 1.54e-65 209
Msa0189680 AT2G18150.1 35.714 336 186 8 2 312 6 336 1.31e-64 207
Msa0189680 AT5G06730.1 36.391 327 185 8 4 312 13 334 7.12e-64 206
Msa0189680 AT4G37530.1 39.432 317 164 8 17 312 20 329 1.33e-63 204
Msa0189680 AT1G14550.1 37.576 330 179 7 1 312 1 321 4.45e-63 202
Msa0189680 AT2G18140.1 35.224 335 188 8 2 312 6 335 4.84e-63 203
Msa0189680 AT3G03670.1 37.654 324 176 7 7 312 6 321 7.15e-63 202
Msa0189680 AT3G32980.1 37.576 330 179 9 5 312 10 334 1.34e-62 202
Msa0189680 AT1G14540.1 38.110 328 174 8 1 312 1 315 1.56e-62 201
Msa0189680 AT5G05340.1 39.482 309 159 9 21 312 27 324 1.78e-62 201
Msa0189680 AT3G49960.1 39.403 335 174 11 1 312 1 329 2.49e-62 201
Msa0189680 AT4G08780.1 37.700 313 170 8 20 312 20 327 1.18e-61 199
Msa0189680 AT4G37520.1 38.065 310 168 7 22 312 25 329 1.74e-61 199
Msa0189680 AT1G34510.1 38.125 320 167 8 8 312 6 309 1.91e-61 198
Msa0189680 AT4G30170.1 36.928 306 173 5 22 312 25 325 2.98e-61 198
Msa0189680 AT5G19890.1 36.474 329 183 10 1 312 8 327 3.27e-61 198
Msa0189680 AT5G67400.1 38.507 335 177 10 1 312 1 329 1.22e-60 196
Msa0189680 AT3G49110.1 37.380 313 171 8 20 312 29 336 1.26e-59 194
Msa0189680 AT2G18980.1 35.714 322 186 6 7 312 7 323 2.60e-59 193
Msa0189680 AT4G37520.2 38.065 310 165 8 22 312 25 326 3.46e-59 192
Msa0189680 AT5G58400.1 38.509 322 166 10 6 308 13 321 5.18e-59 192
Msa0189680 AT5G06720.1 35.692 325 186 9 6 312 14 333 5.31e-59 192
Msa0189680 AT5G66390.1 36.977 311 174 7 19 312 28 333 7.70e-59 192
Msa0189680 AT5G22410.1 37.578 322 173 9 7 308 8 321 1.15e-58 191
Msa0189680 AT4G08770.1 36.792 318 176 8 15 312 15 327 1.17e-58 192
Msa0189680 AT5G51890.1 35.987 314 178 8 5 301 7 314 1.67e-58 191
Msa0189680 AT5G42180.1 34.700 317 187 5 6 307 5 316 2.72e-58 190
Msa0189680 AT3G49120.1 36.741 313 173 8 20 312 28 335 3.08e-57 188
Msa0189680 AT5G19880.1 37.117 326 177 8 10 312 9 329 1.17e-56 186
Msa0189680 AT2G38390.1 35.110 319 183 8 13 312 20 333 1.19e-55 184
Msa0189680 AT2G38380.1 34.985 323 186 8 9 312 16 333 3.04e-55 183
Msa0189680 AT3G50990.1 34.940 332 183 9 7 312 17 341 5.23e-55 182
Msa0189680 AT5G14130.1 35.976 328 184 9 1 312 13 330 2.65e-54 180
Msa0189680 AT4G33870.1 32.000 350 182 10 11 309 54 398 3.92e-54 182
Msa0189680 AT5G14130.2 35.976 328 184 9 1 312 54 371 7.79e-54 180
Msa0189680 AT5G58390.1 36.364 319 172 8 9 308 6 312 4.74e-53 176
Msa0189680 AT1G44970.1 37.217 309 171 7 22 312 43 346 8.09e-53 177
Msa0189680 AT4G16270.1 36.601 306 170 8 24 312 50 348 8.42e-53 177
Msa0189680 AT2G43480.1 37.578 322 165 12 15 312 26 335 3.80e-51 172
Msa0189680 AT5G24070.1 34.795 342 186 9 1 312 1 335 9.48e-51 171
Msa0189680 AT1G68850.1 32.945 343 186 13 6 312 1 335 2.29e-50 170
Msa0189680 AT4G31760.1 34.062 320 186 6 10 312 15 326 3.55e-50 169
Msa0189680 AT4G17690.1 36.964 303 163 10 28 312 30 322 3.82e-50 169
Msa0189680 AT5G47000.1 36.336 333 173 11 4 312 13 330 5.32e-50 169
Msa0189680 AT4G33420.1 35.762 302 168 8 24 312 37 325 4.71e-49 166
Msa0189680 AT1G71695.1 34.967 306 172 9 24 312 44 339 1.23e-48 166
Msa0189680 AT2G24800.1 35.065 308 176 7 22 312 29 329 1.26e-48 165
Msa0189680 AT4G33420.2 35.762 302 168 8 24 312 84 372 2.15e-48 166
Msa0189680 AT5G39580.2 48.503 167 83 2 4 170 7 170 4.04e-48 161
Msa0189680 AT2G35380.1 34.615 338 181 9 5 312 8 335 1.18e-47 163
Msa0189680 AT2G34060.1 36.452 310 171 7 23 311 41 345 1.59e-47 163
Msa0189680 AT4G37530.2 38.077 260 138 7 17 255 20 277 7.92e-47 159
Msa0189680 AT3G28200.1 34.824 313 171 10 20 312 17 316 3.42e-46 159
Msa0189680 AT1G30870.1 33.993 303 178 8 24 312 51 345 1.29e-45 158
Msa0189680 AT2G22420.1 31.562 320 195 8 9 312 8 319 8.16e-45 155
Msa0189680 AT5G40150.1 34.385 317 182 7 13 312 21 328 8.86e-45 155
Msa0189680 AT2G39040.1 33.438 317 177 9 21 312 43 350 1.70e-43 152
Msa0189680 AT4G21960.1 31.373 306 184 6 24 312 31 327 4.21e-42 148
Msa0189680 AT1G24110.1 32.154 311 185 6 21 312 19 322 7.23e-42 147
Msa0189680 AT3G17070.1 30.275 327 199 7 4 308 16 335 6.99e-37 135
Msa0189680 AT2G24800.2 41.899 179 97 4 22 194 29 206 1.81e-36 131
Msa0189680 AT2G37130.1 30.032 313 182 9 23 312 29 327 3.86e-33 124
Msa0189680 AT2G37130.2 30.000 310 180 9 26 312 1 296 1.76e-32 122
Msa0189680 AT2G35380.2 31.496 254 139 7 84 312 4 247 3.92e-26 104
Msa0189680 AT3G17070.2 28.897 263 159 7 66 308 18 272 1.54e-22 95.1

Find 58 sgRNAs with CRISPR-Local

Find 244 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGGCACCTTGCTTTCAGTTC+TGG 0.179644 2_1:-5145640 None:intergenic
GGTCCAAATGTTGGATTTAT+AGG 0.216242 2_1:-5145829 None:intergenic
GGCACCTTGCTTTCAGTTCT+GGG 0.266441 2_1:-5145639 None:intergenic
TCAGTTCTGGGAGGAAATTC+AGG 0.277375 2_1:-5145627 None:intergenic
TTGTTGATATCTCCATCCTT+AGG 0.338339 2_1:-5145661 None:intergenic
AATCCTATAAATCCAACATT+TGG 0.339432 2_1:+5145826 Msa0189680:CDS
TTATTCTTAATCTTAATTGA+TGG 0.347013 2_1:+5143780 Msa0189680:CDS
CCATGGCTGCTGTTCTTCTC+AGG 0.361244 2_1:+5143904 Msa0189680:CDS
TGTGGAGATGCAGTCTTCTC+TGG 0.367035 2_1:-5144075 None:intergenic
TCTCCACATGCAGGTGTTAG+AGG 0.385000 2_1:+5144090 Msa0189680:CDS
AATTCAGCTGCTCATACAAT+TGG 0.400962 2_1:+5145532 Msa0189680:intron
AGGAAATTCAGGTTAATAGT+TGG 0.429063 2_1:-5145616 None:intergenic
TGTTGATATCTCCATCCTTA+GGG 0.431103 2_1:-5145660 None:intergenic
CAGGTTCTGTTGGAAACATT+AGG 0.432651 2_1:+5145911 Msa0189680:CDS
GGAAATTCAGGTTAATAGTT+GGG 0.434698 2_1:-5145615 None:intergenic
TTGTGCAGATATTGTTGCTT+TGG 0.448468 2_1:+5144173 Msa0189680:CDS
CCTGCATATCAAGTTCCAAC+AGG 0.455143 2_1:+5144365 Msa0189680:CDS
CCTGTTGGAACTTGATATGC+AGG 0.459129 2_1:-5144365 None:intergenic
GAGACCCTTGTTCAGAAACT+TGG 0.470684 2_1:-5144468 None:intergenic
TGATGTTACATGTAGGCAAA+TGG 0.475836 2_1:+5144341 Msa0189680:intron
ATAGTTGGGTCTGATCCAAA+TGG 0.477620 2_1:-5145601 None:intergenic
TGGATTTATAGGATTAAAGT+AGG 0.481291 2_1:-5145818 None:intergenic
AACAGGGAGAAGAGATGGAT+TGG 0.483056 2_1:+5144382 Msa0189680:CDS
AGATGCTTCTAATTGTGCCT+TGG 0.487334 2_1:-5144133 None:intergenic
TCAAAAGAAGGTCCAAATGT+TGG 0.498612 2_1:-5145838 None:intergenic
AAACGTTTAGCCATGGATGA+AGG 0.499351 2_1:+5145682 Msa0189680:CDS
GGAGTAAAGACAGGTTCTGT+TGG 0.500900 2_1:+5145901 Msa0189680:CDS
AATCCATCTCTTCTCCCTGT+TGG 0.503118 2_1:-5144380 None:intergenic
TCTGCACAAGAAACCACTCC+TGG 0.512606 2_1:-5144159 None:intergenic
CAAGGCCAAGTTTCTGAACA+AGG 0.517501 2_1:+5144463 Msa0189680:CDS
ATCTCTTGCAGATAATATGC+CGG 0.534359 2_1:+5144415 Msa0189680:CDS
CAGATCACTCCCTTCATCCA+TGG 0.535133 2_1:-5145692 None:intergenic
TGAATTGAATCATCAACATC+CGG 0.542004 2_1:-5144434 None:intergenic
TCTAAGAGTCAGCTTCATGT+TGG 0.543325 2_1:+5143804 Msa0189680:CDS
CCTGAGAAGAACAGCAGCCA+TGG 0.549117 2_1:-5143904 None:intergenic
TAGTTGGGTCTGATCCAAAT+GGG 0.549334 2_1:-5145600 None:intergenic
GTTCCAACAGGGAGAAGAGA+TGG 0.549752 2_1:+5144377 Msa0189680:CDS
CAATTAGAAGCATCTTGTCC+AGG 0.557883 2_1:+5144141 Msa0189680:CDS
CAGCTTCTCTAACAACATCA+TGG 0.559846 2_1:-5143866 None:intergenic
GGACAAATTGGAGTAAAGAC+AGG 0.565783 2_1:+5145892 Msa0189680:CDS
AAAGCAAGGTGCCCTAAGGA+TGG 0.570656 2_1:+5145649 Msa0189680:CDS
TCCTCCCAGAACTGAAAGCA+AGG 0.572671 2_1:+5145635 Msa0189680:CDS
AACGTTTAGCCATGGATGAA+GGG 0.576082 2_1:+5145683 Msa0189680:CDS
ACTGAAAGCAAGGTGCCCTA+AGG 0.579558 2_1:+5145645 Msa0189680:CDS
AGAAGCATCTTGTCCAGGAG+TGG 0.580324 2_1:+5144146 Msa0189680:CDS
AAGACTGCATCTCCACATGC+AGG 0.590000 2_1:+5144081 Msa0189680:CDS
TATCAACAAACGTTTAGCCA+TGG 0.597225 2_1:+5145675 Msa0189680:CDS
AGCAAGAGATGCAATTGTCA+TGG 0.601325 2_1:+5144197 Msa0189680:CDS
TGATTCAATTCAGCAACTCA+AGG 0.601761 2_1:+5144445 Msa0189680:CDS
CTCTACTTGAGGACATGTGT+TGG 0.603991 2_1:-5143835 None:intergenic
TCTCATAAAGAAGCATGCTG+TGG 0.627216 2_1:-5145557 None:intergenic
TATCCTCTAACACCTGCATG+TGG 0.631759 2_1:-5144093 None:intergenic
TGTTCTTTCTGACAGAACCA+TGG 0.637868 2_1:+5143887 Msa0189680:CDS
ACCTTGCTTTCAGTTCTGGG+AGG 0.637941 2_1:-5145636 None:intergenic
TGGACAGTGGACTCTACTTG+AGG 0.644794 2_1:-5143846 None:intergenic
TCTAACAACATCATGGACAG+TGG 0.665227 2_1:-5143859 None:intergenic
CTGCATATCAAGTTCCAACA+GGG 0.673250 2_1:+5144366 Msa0189680:CDS
AAGGCCAAGTTTCTGAACAA+GGG 0.675709 2_1:+5144464 Msa0189680:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGTATATTGTTTTTTTTTTT+TGG + chr2_1:5145444-5145463 Msa0189680:intron 10.0%
!!! TGTATATTGTTTTTTTTTTT+TGG + chr2_1:5145444-5145463 Msa0189680:intron 10.0%
!! TTATTCTTAATCTTAATTGA+TGG + chr2_1:5143780-5143799 Msa0189680:CDS 15.0%
!!! AAAATCTTTAAAAAAGTGAT+TGG - chr2_1:5144935-5144954 None:intergenic 15.0%
!!! ATCCTTTGTTTTATTTAATT+GGG - chr2_1:5145029-5145048 None:intergenic 15.0%
!! AAAGAATGTTATGATAAAAT+AGG - chr2_1:5145170-5145189 None:intergenic 15.0%
!! TTATTCTTAATCTTAATTGA+TGG + chr2_1:5143780-5143799 Msa0189680:CDS 15.0%
!!! AAAATCTTTAAAAAAGTGAT+TGG - chr2_1:5144935-5144954 None:intergenic 15.0%
!!! ATCCTTTGTTTTATTTAATT+GGG - chr2_1:5145029-5145048 None:intergenic 15.0%
!! AAAGAATGTTATGATAAAAT+AGG - chr2_1:5145170-5145189 None:intergenic 15.0%
!!! AAAGATTTTGTCAATTTTTC+AGG + chr2_1:5144946-5144965 Msa0189680:intron 20.0%
!!! GATCCTTTGTTTTATTTAAT+TGG - chr2_1:5145030-5145049 None:intergenic 20.0%
!! TTAAATAAAACAAAGGATCT+TGG + chr2_1:5145031-5145050 Msa0189680:intron 20.0%
!! TAAATAAAACAAAGGATCTT+GGG + chr2_1:5145032-5145051 Msa0189680:intron 20.0%
!!! TTTGGCTTATAAAATTCAAA+CGG + chr2_1:5145120-5145139 Msa0189680:intron 20.0%
!!! AAAATTCTAAAATGTGCAAA+AGG - chr2_1:5145267-5145286 None:intergenic 20.0%
!! TCAACATATTTATATCAGAA+AGG + chr2_1:5145298-5145317 Msa0189680:intron 20.0%
!! ATATCAATTAAACTCTTGTA+CGG + chr2_1:5145389-5145408 Msa0189680:intron 20.0%
!! AGAGTATATTGAAAAACATA+AGG + chr2_1:5145725-5145744 Msa0189680:CDS 20.0%
!! GAGTATATTGAAAAACATAA+GGG + chr2_1:5145726-5145745 Msa0189680:CDS 20.0%
!!! ACAAAATCATTTTCAAAAGA+AGG - chr2_1:5145853-5145872 None:intergenic 20.0%
!!! AAAGATTTTGTCAATTTTTC+AGG + chr2_1:5144946-5144965 Msa0189680:intron 20.0%
!!! GATCCTTTGTTTTATTTAAT+TGG - chr2_1:5145030-5145049 None:intergenic 20.0%
!! TTAAATAAAACAAAGGATCT+TGG + chr2_1:5145031-5145050 Msa0189680:intron 20.0%
!! TAAATAAAACAAAGGATCTT+GGG + chr2_1:5145032-5145051 Msa0189680:intron 20.0%
!!! TTTGGCTTATAAAATTCAAA+CGG + chr2_1:5145120-5145139 Msa0189680:intron 20.0%
!!! AAAATTCTAAAATGTGCAAA+AGG - chr2_1:5145267-5145286 None:intergenic 20.0%
!! TCAACATATTTATATCAGAA+AGG + chr2_1:5145298-5145317 Msa0189680:intron 20.0%
!! ATATCAATTAAACTCTTGTA+CGG + chr2_1:5145389-5145408 Msa0189680:intron 20.0%
!! AGAGTATATTGAAAAACATA+AGG + chr2_1:5145725-5145744 Msa0189680:CDS 20.0%
!! GAGTATATTGAAAAACATAA+GGG + chr2_1:5145726-5145745 Msa0189680:CDS 20.0%
!!! ACAAAATCATTTTCAAAAGA+AGG - chr2_1:5145853-5145872 None:intergenic 20.0%
! TTGAGGTCTTTCTATATATA+TGG + chr2_1:5143949-5143968 Msa0189680:intron 25.0%
!!! TGATTTTGATTGAGTTTTTC+AGG + chr2_1:5144014-5144033 Msa0189680:intron 25.0%
!!! GATTTTGATTGAGTTTTTCA+GGG + chr2_1:5144015-5144034 Msa0189680:intron 25.0%
! ATTAGAGTAAGTTAGGTATT+AGG - chr2_1:5144224-5144243 None:intergenic 25.0%
! ACACTTATTGAATCATAAAC+AGG - chr2_1:5144317-5144336 None:intergenic 25.0%
! TGTTTATGATTCAATAAGTG+TGG + chr2_1:5144316-5144335 Msa0189680:intron 25.0%
! AAAGTAATATGAACTAGAAC+AGG + chr2_1:5144775-5144794 Msa0189680:intron 25.0%
! AAATAATCACAACAAGACAT+TGG + chr2_1:5144812-5144831 Msa0189680:intron 25.0%
! AATTGAGTAAATCATGTAGT+TGG - chr2_1:5144843-5144862 None:intergenic 25.0%
! ACTACATGATTTACTCAATT+TGG + chr2_1:5144843-5144862 Msa0189680:intron 25.0%
! CTACATGATTTACTCAATTT+GGG + chr2_1:5144844-5144863 Msa0189680:intron 25.0%
!! TCTTTAAAAAAGTGATTGGT+TGG - chr2_1:5144931-5144950 None:intergenic 25.0%
! ATATTGAAAAACATAAGGGA+AGG + chr2_1:5145730-5145749 Msa0189680:CDS 25.0%
! TGGATTTATAGGATTAAAGT+AGG - chr2_1:5145821-5145840 None:intergenic 25.0%
! AATCCTATAAATCCAACATT+TGG + chr2_1:5145826-5145845 Msa0189680:CDS 25.0%
!! AAATGATTTTGTTCAGTCAA+TGG + chr2_1:5145861-5145880 Msa0189680:CDS 25.0%
! TTGAGGTCTTTCTATATATA+TGG + chr2_1:5143949-5143968 Msa0189680:intron 25.0%
!!! TGATTTTGATTGAGTTTTTC+AGG + chr2_1:5144014-5144033 Msa0189680:intron 25.0%
!!! GATTTTGATTGAGTTTTTCA+GGG + chr2_1:5144015-5144034 Msa0189680:intron 25.0%
! ATTAGAGTAAGTTAGGTATT+AGG - chr2_1:5144224-5144243 None:intergenic 25.0%
! ACACTTATTGAATCATAAAC+AGG - chr2_1:5144317-5144336 None:intergenic 25.0%
! TGTTTATGATTCAATAAGTG+TGG + chr2_1:5144316-5144335 Msa0189680:intron 25.0%
! AAAGTAATATGAACTAGAAC+AGG + chr2_1:5144775-5144794 Msa0189680:intron 25.0%
! AAATAATCACAACAAGACAT+TGG + chr2_1:5144812-5144831 Msa0189680:intron 25.0%
! AATTGAGTAAATCATGTAGT+TGG - chr2_1:5144843-5144862 None:intergenic 25.0%
! ACTACATGATTTACTCAATT+TGG + chr2_1:5144843-5144862 Msa0189680:intron 25.0%
! CTACATGATTTACTCAATTT+GGG + chr2_1:5144844-5144863 Msa0189680:intron 25.0%
!! TCTTTAAAAAAGTGATTGGT+TGG - chr2_1:5144931-5144950 None:intergenic 25.0%
! ATATTGAAAAACATAAGGGA+AGG + chr2_1:5145730-5145749 Msa0189680:CDS 25.0%
! TGGATTTATAGGATTAAAGT+AGG - chr2_1:5145821-5145840 None:intergenic 25.0%
! AATCCTATAAATCCAACATT+TGG + chr2_1:5145826-5145845 Msa0189680:CDS 25.0%
!! AAATGATTTTGTTCAGTCAA+TGG + chr2_1:5145861-5145880 Msa0189680:CDS 25.0%
ATGTGGTATTAGAGTAAGTT+AGG - chr2_1:5144231-5144250 None:intergenic 30.0%
GAAAAGATTTGTGACTAATG+TGG - chr2_1:5144248-5144267 None:intergenic 30.0%
TGAATTGAATCATCAACATC+CGG - chr2_1:5144437-5144456 None:intergenic 30.0%
! AGTAAATCATGTAGTTGGTA+TGG - chr2_1:5144838-5144857 None:intergenic 30.0%
ATTTGGGAAATTGATAGAGA+AGG + chr2_1:5144860-5144879 Msa0189680:intron 30.0%
TTTGGGAAATTGATAGAGAA+GGG + chr2_1:5144861-5144880 Msa0189680:intron 30.0%
GGCCCAATTAAATAAAACAA+AGG + chr2_1:5145024-5145043 Msa0189680:intron 30.0%
CTCAATAGAATGGAAAAAGA+AGG - chr2_1:5145080-5145099 None:intergenic 30.0%
AAGCCAAATTACATAAAACG+CGG - chr2_1:5145108-5145127 None:intergenic 30.0%
CATCACATTCTTATTACTAG+AGG + chr2_1:5145209-5145228 Msa0189680:intron 30.0%
TGGTTGTAAATCCAGAAAAA+AGG - chr2_1:5145239-5145258 None:intergenic 30.0%
AAAATGTGCAAAAGGAAAGT+TGG - chr2_1:5145259-5145278 None:intergenic 30.0%
AGATCAGTGAACAATTACTA+TGG - chr2_1:5145500-5145519 None:intergenic 30.0%
!! AGCAGCTGAATTTTTGTTTA+TGG - chr2_1:5145524-5145543 None:intergenic 30.0%
! CTATACGAATTTTTCCCATT+TGG + chr2_1:5145586-5145605 Msa0189680:CDS 30.0%
! GGAAATTCAGGTTAATAGTT+GGG - chr2_1:5145618-5145637 None:intergenic 30.0%
! AGGAAATTCAGGTTAATAGT+TGG - chr2_1:5145619-5145638 None:intergenic 30.0%
TGTTCAGTCAATGGTAAAAA+TGG + chr2_1:5145870-5145889 Msa0189680:CDS 30.0%
GTTCAGTCAATGGTAAAAAT+GGG + chr2_1:5145871-5145890 Msa0189680:CDS 30.0%
ATGGTAAAAATGGGACAAAT+TGG + chr2_1:5145880-5145899 Msa0189680:CDS 30.0%
ATGTGGTATTAGAGTAAGTT+AGG - chr2_1:5144231-5144250 None:intergenic 30.0%
GAAAAGATTTGTGACTAATG+TGG - chr2_1:5144248-5144267 None:intergenic 30.0%
TGAATTGAATCATCAACATC+CGG - chr2_1:5144437-5144456 None:intergenic 30.0%
! AGTAAATCATGTAGTTGGTA+TGG - chr2_1:5144838-5144857 None:intergenic 30.0%
ATTTGGGAAATTGATAGAGA+AGG + chr2_1:5144860-5144879 Msa0189680:intron 30.0%
TTTGGGAAATTGATAGAGAA+GGG + chr2_1:5144861-5144880 Msa0189680:intron 30.0%
GGCCCAATTAAATAAAACAA+AGG + chr2_1:5145024-5145043 Msa0189680:intron 30.0%
CTCAATAGAATGGAAAAAGA+AGG - chr2_1:5145080-5145099 None:intergenic 30.0%
AAGCCAAATTACATAAAACG+CGG - chr2_1:5145108-5145127 None:intergenic 30.0%
CATCACATTCTTATTACTAG+AGG + chr2_1:5145209-5145228 Msa0189680:intron 30.0%
TGGTTGTAAATCCAGAAAAA+AGG - chr2_1:5145239-5145258 None:intergenic 30.0%
AAAATGTGCAAAAGGAAAGT+TGG - chr2_1:5145259-5145278 None:intergenic 30.0%
AGATCAGTGAACAATTACTA+TGG - chr2_1:5145500-5145519 None:intergenic 30.0%
!! AGCAGCTGAATTTTTGTTTA+TGG - chr2_1:5145524-5145543 None:intergenic 30.0%
! CTATACGAATTTTTCCCATT+TGG + chr2_1:5145586-5145605 Msa0189680:CDS 30.0%
! GGAAATTCAGGTTAATAGTT+GGG - chr2_1:5145618-5145637 None:intergenic 30.0%
! AGGAAATTCAGGTTAATAGT+TGG - chr2_1:5145619-5145638 None:intergenic 30.0%
TGTTCAGTCAATGGTAAAAA+TGG + chr2_1:5145870-5145889 Msa0189680:CDS 30.0%
GTTCAGTCAATGGTAAAAAT+GGG + chr2_1:5145871-5145890 Msa0189680:CDS 30.0%
ATGGTAAAAATGGGACAAAT+TGG + chr2_1:5145880-5145899 Msa0189680:CDS 30.0%
!! TTTTCATGACTGCTTTGTTG+AGG + chr2_1:5143932-5143951 Msa0189680:CDS 35.0%
TGATGTGATAGAAAAAGCCA+AGG + chr2_1:5144116-5144135 Msa0189680:CDS 35.0%
! TTGTGCAGATATTGTTGCTT+TGG + chr2_1:5144173-5144192 Msa0189680:CDS 35.0%
TGTGGAATGATGTTACATGT+AGG + chr2_1:5144334-5144353 Msa0189680:intron 35.0%
TGATGTTACATGTAGGCAAA+TGG + chr2_1:5144341-5144360 Msa0189680:intron 35.0%
ATCTCTTGCAGATAATATGC+CGG + chr2_1:5144415-5144434 Msa0189680:CDS 35.0%
TGATTCAATTCAGCAACTCA+AGG + chr2_1:5144445-5144464 Msa0189680:CDS 35.0%
ATGCAGAGTAAATGAGATCA+TGG + chr2_1:5144739-5144758 Msa0189680:intron 35.0%
TTGTTTAATGAGCCTGTTAC+TGG + chr2_1:5145003-5145022 Msa0189680:intron 35.0%
TATTTAATTGGGCCAGTAAC+AGG - chr2_1:5145018-5145037 None:intergenic 35.0%
! TCTCCGCGTTTTATGTAATT+TGG + chr2_1:5145102-5145121 Msa0189680:intron 35.0%
!!! CTAGAGGATAACCTTTTTTC+TGG + chr2_1:5145225-5145244 Msa0189680:intron 35.0%
AATTCAGCTGCTCATACAAT+TGG + chr2_1:5145532-5145551 Msa0189680:intron 35.0%
TGTTGATATCTCCATCCTTA+GGG - chr2_1:5145663-5145682 None:intergenic 35.0%
TTGTTGATATCTCCATCCTT+AGG - chr2_1:5145664-5145683 None:intergenic 35.0%
TATCAACAAACGTTTAGCCA+TGG + chr2_1:5145675-5145694 Msa0189680:CDS 35.0%
GGTCCAAATGTTGGATTTAT+AGG - chr2_1:5145832-5145851 None:intergenic 35.0%
TCAAAAGAAGGTCCAAATGT+TGG - chr2_1:5145841-5145860 None:intergenic 35.0%
!! TTTTCATGACTGCTTTGTTG+AGG + chr2_1:5143932-5143951 Msa0189680:CDS 35.0%
TGATGTGATAGAAAAAGCCA+AGG + chr2_1:5144116-5144135 Msa0189680:CDS 35.0%
! TTGTGCAGATATTGTTGCTT+TGG + chr2_1:5144173-5144192 Msa0189680:CDS 35.0%
TGTGGAATGATGTTACATGT+AGG + chr2_1:5144334-5144353 Msa0189680:intron 35.0%
TGATGTTACATGTAGGCAAA+TGG + chr2_1:5144341-5144360 Msa0189680:intron 35.0%
ATCTCTTGCAGATAATATGC+CGG + chr2_1:5144415-5144434 Msa0189680:CDS 35.0%
TGATTCAATTCAGCAACTCA+AGG + chr2_1:5144445-5144464 Msa0189680:CDS 35.0%
ATGCAGAGTAAATGAGATCA+TGG + chr2_1:5144739-5144758 Msa0189680:intron 35.0%
TTGTTTAATGAGCCTGTTAC+TGG + chr2_1:5145003-5145022 Msa0189680:intron 35.0%
TATTTAATTGGGCCAGTAAC+AGG - chr2_1:5145018-5145037 None:intergenic 35.0%
! TCTCCGCGTTTTATGTAATT+TGG + chr2_1:5145102-5145121 Msa0189680:intron 35.0%
!!! CTAGAGGATAACCTTTTTTC+TGG + chr2_1:5145225-5145244 Msa0189680:intron 35.0%
AATTCAGCTGCTCATACAAT+TGG + chr2_1:5145532-5145551 Msa0189680:intron 35.0%
TGTTGATATCTCCATCCTTA+GGG - chr2_1:5145663-5145682 None:intergenic 35.0%
TTGTTGATATCTCCATCCTT+AGG - chr2_1:5145664-5145683 None:intergenic 35.0%
TATCAACAAACGTTTAGCCA+TGG + chr2_1:5145675-5145694 Msa0189680:CDS 35.0%
GGTCCAAATGTTGGATTTAT+AGG - chr2_1:5145832-5145851 None:intergenic 35.0%
TCAAAAGAAGGTCCAAATGT+TGG - chr2_1:5145841-5145860 None:intergenic 35.0%
TCTAAGAGTCAGCTTCATGT+TGG + chr2_1:5143804-5143823 Msa0189680:CDS 40.0%
TCTAACAACATCATGGACAG+TGG - chr2_1:5143862-5143881 None:intergenic 40.0%
CAGCTTCTCTAACAACATCA+TGG - chr2_1:5143869-5143888 None:intergenic 40.0%
TGTTCTTTCTGACAGAACCA+TGG + chr2_1:5143887-5143906 Msa0189680:CDS 40.0%
!!! TGAGTTTTTCAGGGATGTGA+TGG + chr2_1:5144024-5144043 Msa0189680:intron 40.0%
AGATGCTTCTAATTGTGCCT+TGG - chr2_1:5144136-5144155 None:intergenic 40.0%
!! CAATTAGAAGCATCTTGTCC+AGG + chr2_1:5144141-5144160 Msa0189680:CDS 40.0%
AGCAAGAGATGCAATTGTCA+TGG + chr2_1:5144197-5144216 Msa0189680:CDS 40.0%
CTGCATATCAAGTTCCAACA+GGG + chr2_1:5144366-5144385 Msa0189680:CDS 40.0%
AAGGCCAAGTTTCTGAACAA+GGG + chr2_1:5144464-5144483 Msa0189680:CDS 40.0%
AAAGACCTTGTTCTTCTCAG+TGG + chr2_1:5144497-5144516 Msa0189680:intron 40.0%
TGAGATCATGGTTGTTGAAG+TGG + chr2_1:5144751-5144770 Msa0189680:intron 40.0%
CAGGCTCATTAAACAAAGAG+TGG - chr2_1:5144999-5145018 None:intergenic 40.0%
!!! GGGTCTCTAGGATTATGTTT+TGG + chr2_1:5145052-5145071 Msa0189680:intron 40.0%
! TCTCATAAAGAAGCATGCTG+TGG - chr2_1:5145560-5145579 None:intergenic 40.0%
TAGTTGGGTCTGATCCAAAT+GGG - chr2_1:5145603-5145622 None:intergenic 40.0%
ATAGTTGGGTCTGATCCAAA+TGG - chr2_1:5145604-5145623 None:intergenic 40.0%
AAACGTTTAGCCATGGATGA+AGG + chr2_1:5145682-5145701 Msa0189680:CDS 40.0%
AACGTTTAGCCATGGATGAA+GGG + chr2_1:5145683-5145702 Msa0189680:CDS 40.0%
GGACAAATTGGAGTAAAGAC+AGG + chr2_1:5145892-5145911 Msa0189680:CDS 40.0%
CAGGTTCTGTTGGAAACATT+AGG + chr2_1:5145911-5145930 Msa0189680:CDS 40.0%
TCTAAGAGTCAGCTTCATGT+TGG + chr2_1:5143804-5143823 Msa0189680:CDS 40.0%
TCTAACAACATCATGGACAG+TGG - chr2_1:5143862-5143881 None:intergenic 40.0%
CAGCTTCTCTAACAACATCA+TGG - chr2_1:5143869-5143888 None:intergenic 40.0%
TGTTCTTTCTGACAGAACCA+TGG + chr2_1:5143887-5143906 Msa0189680:CDS 40.0%
!!! TGAGTTTTTCAGGGATGTGA+TGG + chr2_1:5144024-5144043 Msa0189680:intron 40.0%
AGATGCTTCTAATTGTGCCT+TGG - chr2_1:5144136-5144155 None:intergenic 40.0%
!! CAATTAGAAGCATCTTGTCC+AGG + chr2_1:5144141-5144160 Msa0189680:CDS 40.0%
AGCAAGAGATGCAATTGTCA+TGG + chr2_1:5144197-5144216 Msa0189680:CDS 40.0%
CTGCATATCAAGTTCCAACA+GGG + chr2_1:5144366-5144385 Msa0189680:CDS 40.0%
AAGGCCAAGTTTCTGAACAA+GGG + chr2_1:5144464-5144483 Msa0189680:CDS 40.0%
AAAGACCTTGTTCTTCTCAG+TGG + chr2_1:5144497-5144516 Msa0189680:intron 40.0%
TGAGATCATGGTTGTTGAAG+TGG + chr2_1:5144751-5144770 Msa0189680:intron 40.0%
CAGGCTCATTAAACAAAGAG+TGG - chr2_1:5144999-5145018 None:intergenic 40.0%
!!! GGGTCTCTAGGATTATGTTT+TGG + chr2_1:5145052-5145071 Msa0189680:intron 40.0%
! TCTCATAAAGAAGCATGCTG+TGG - chr2_1:5145560-5145579 None:intergenic 40.0%
TAGTTGGGTCTGATCCAAAT+GGG - chr2_1:5145603-5145622 None:intergenic 40.0%
ATAGTTGGGTCTGATCCAAA+TGG - chr2_1:5145604-5145623 None:intergenic 40.0%
AAACGTTTAGCCATGGATGA+AGG + chr2_1:5145682-5145701 Msa0189680:CDS 40.0%
AACGTTTAGCCATGGATGAA+GGG + chr2_1:5145683-5145702 Msa0189680:CDS 40.0%
GGACAAATTGGAGTAAAGAC+AGG + chr2_1:5145892-5145911 Msa0189680:CDS 40.0%
CAGGTTCTGTTGGAAACATT+AGG + chr2_1:5145911-5145930 Msa0189680:CDS 40.0%
CTCTACTTGAGGACATGTGT+TGG - chr2_1:5143838-5143857 None:intergenic 45.0%
TATCCTCTAACACCTGCATG+TGG - chr2_1:5144096-5144115 None:intergenic 45.0%
CCTGTTGGAACTTGATATGC+AGG - chr2_1:5144368-5144387 None:intergenic 45.0%
CCTGCATATCAAGTTCCAAC+AGG + chr2_1:5144365-5144384 Msa0189680:CDS 45.0%
AATCCATCTCTTCTCCCTGT+TGG - chr2_1:5144383-5144402 None:intergenic 45.0%
AACAGGGAGAAGAGATGGAT+TGG + chr2_1:5144382-5144401 Msa0189680:CDS 45.0%
CAAGGCCAAGTTTCTGAACA+AGG + chr2_1:5144463-5144482 Msa0189680:CDS 45.0%
GAGACCCTTGTTCAGAAACT+TGG - chr2_1:5144471-5144490 None:intergenic 45.0%
GTGTACCACTGAGAAGAACA+AGG - chr2_1:5144505-5144524 None:intergenic 45.0%
GACATGATACACGTCGATAC+TGG + chr2_1:5144580-5144599 Msa0189680:intron 45.0%
ACAAAGGATCTTGGGTCTCT+AGG + chr2_1:5145040-5145059 Msa0189680:intron 45.0%
CGCGGAGAAACTCAATAGAA+TGG - chr2_1:5145090-5145109 None:intergenic 45.0%
!! TCAGTTCTGGGAGGAAATTC+AGG - chr2_1:5145630-5145649 None:intergenic 45.0%
GGAGTAAAGACAGGTTCTGT+TGG + chr2_1:5145901-5145920 Msa0189680:CDS 45.0%
CTCTACTTGAGGACATGTGT+TGG - chr2_1:5143838-5143857 None:intergenic 45.0%
TATCCTCTAACACCTGCATG+TGG - chr2_1:5144096-5144115 None:intergenic 45.0%
CCTGTTGGAACTTGATATGC+AGG - chr2_1:5144368-5144387 None:intergenic 45.0%
CCTGCATATCAAGTTCCAAC+AGG + chr2_1:5144365-5144384 Msa0189680:CDS 45.0%
AATCCATCTCTTCTCCCTGT+TGG - chr2_1:5144383-5144402 None:intergenic 45.0%
AACAGGGAGAAGAGATGGAT+TGG + chr2_1:5144382-5144401 Msa0189680:CDS 45.0%
CAAGGCCAAGTTTCTGAACA+AGG + chr2_1:5144463-5144482 Msa0189680:CDS 45.0%
GAGACCCTTGTTCAGAAACT+TGG - chr2_1:5144471-5144490 None:intergenic 45.0%
GTGTACCACTGAGAAGAACA+AGG - chr2_1:5144505-5144524 None:intergenic 45.0%
GACATGATACACGTCGATAC+TGG + chr2_1:5144580-5144599 Msa0189680:intron 45.0%
ACAAAGGATCTTGGGTCTCT+AGG + chr2_1:5145040-5145059 Msa0189680:intron 45.0%
CGCGGAGAAACTCAATAGAA+TGG - chr2_1:5145090-5145109 None:intergenic 45.0%
!! TCAGTTCTGGGAGGAAATTC+AGG - chr2_1:5145630-5145649 None:intergenic 45.0%
GGAGTAAAGACAGGTTCTGT+TGG + chr2_1:5145901-5145920 Msa0189680:CDS 45.0%
!! TTAATTAAATATTATGATAT+AGG + chr2_1:5144894-5144913 Msa0189680:intron 5.0%
!! TTAATTAAATATTATGATAT+AGG + chr2_1:5144894-5144913 Msa0189680:intron 5.0%
! TGGACAGTGGACTCTACTTG+AGG - chr2_1:5143849-5143868 None:intergenic 50.0%
TGTGGAGATGCAGTCTTCTC+TGG - chr2_1:5144078-5144097 None:intergenic 50.0%
AAGACTGCATCTCCACATGC+AGG + chr2_1:5144081-5144100 Msa0189680:CDS 50.0%
TCTCCACATGCAGGTGTTAG+AGG + chr2_1:5144090-5144109 Msa0189680:CDS 50.0%
!! AGAAGCATCTTGTCCAGGAG+TGG + chr2_1:5144146-5144165 Msa0189680:CDS 50.0%
TCTGCACAAGAAACCACTCC+TGG - chr2_1:5144162-5144181 None:intergenic 50.0%
GTTCCAACAGGGAGAAGAGA+TGG + chr2_1:5144377-5144396 Msa0189680:CDS 50.0%
TCCTCCCAGAACTGAAAGCA+AGG + chr2_1:5145635-5145654 Msa0189680:CDS 50.0%
!! ACCTTGCTTTCAGTTCTGGG+AGG - chr2_1:5145639-5145658 None:intergenic 50.0%
GGCACCTTGCTTTCAGTTCT+GGG - chr2_1:5145642-5145661 None:intergenic 50.0%
! ACTGAAAGCAAGGTGCCCTA+AGG + chr2_1:5145645-5145664 Msa0189680:CDS 50.0%
! AAAGCAAGGTGCCCTAAGGA+TGG + chr2_1:5145649-5145668 Msa0189680:CDS 50.0%
CAGATCACTCCCTTCATCCA+TGG - chr2_1:5145695-5145714 None:intergenic 50.0%
! TGGACAGTGGACTCTACTTG+AGG - chr2_1:5143849-5143868 None:intergenic 50.0%
TGTGGAGATGCAGTCTTCTC+TGG - chr2_1:5144078-5144097 None:intergenic 50.0%
AAGACTGCATCTCCACATGC+AGG + chr2_1:5144081-5144100 Msa0189680:CDS 50.0%
TCTCCACATGCAGGTGTTAG+AGG + chr2_1:5144090-5144109 Msa0189680:CDS 50.0%
!! AGAAGCATCTTGTCCAGGAG+TGG + chr2_1:5144146-5144165 Msa0189680:CDS 50.0%
TCTGCACAAGAAACCACTCC+TGG - chr2_1:5144162-5144181 None:intergenic 50.0%
GTTCCAACAGGGAGAAGAGA+TGG + chr2_1:5144377-5144396 Msa0189680:CDS 50.0%
TCCTCCCAGAACTGAAAGCA+AGG + chr2_1:5145635-5145654 Msa0189680:CDS 50.0%
!! ACCTTGCTTTCAGTTCTGGG+AGG - chr2_1:5145639-5145658 None:intergenic 50.0%
GGCACCTTGCTTTCAGTTCT+GGG - chr2_1:5145642-5145661 None:intergenic 50.0%
! ACTGAAAGCAAGGTGCCCTA+AGG + chr2_1:5145645-5145664 Msa0189680:CDS 50.0%
! AAAGCAAGGTGCCCTAAGGA+TGG + chr2_1:5145649-5145668 Msa0189680:CDS 50.0%
CAGATCACTCCCTTCATCCA+TGG - chr2_1:5145695-5145714 None:intergenic 50.0%
CCTGAGAAGAACAGCAGCCA+TGG - chr2_1:5143907-5143926 None:intergenic 55.0%
CCATGGCTGCTGTTCTTCTC+AGG + chr2_1:5143904-5143923 Msa0189680:CDS 55.0%
GGGCACCTTGCTTTCAGTTC+TGG - chr2_1:5145643-5145662 None:intergenic 55.0%
CCTGAGAAGAACAGCAGCCA+TGG - chr2_1:5143907-5143926 None:intergenic 55.0%
CCATGGCTGCTGTTCTTCTC+AGG + chr2_1:5143904-5143923 Msa0189680:CDS 55.0%
GGGCACCTTGCTTTCAGTTC+TGG - chr2_1:5145643-5145662 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 5143747 5145957 5143747 ID=Msa0189680;Name=Msa0189680
chr2_1 mRNA 5143747 5145957 5143747 ID=Msa0189680-mRNA-1;Parent=Msa0189680;Name=Msa0189680-mRNA-1;_AED=0.09;_eAED=0.09;_QI=0|0|0|1|1|1|4|0|312
chr2_1 exon 5143747 5143953 5143747 ID=Msa0189680-mRNA-1:exon:2301;Parent=Msa0189680-mRNA-1
chr2_1 exon 5144036 5144218 5144036 ID=Msa0189680-mRNA-1:exon:2302;Parent=Msa0189680-mRNA-1
chr2_1 exon 5144356 5144485 5144356 ID=Msa0189680-mRNA-1:exon:2303;Parent=Msa0189680-mRNA-1
chr2_1 exon 5145539 5145957 5145539 ID=Msa0189680-mRNA-1:exon:2304;Parent=Msa0189680-mRNA-1
chr2_1 CDS 5143747 5143953 5143747 ID=Msa0189680-mRNA-1:cds;Parent=Msa0189680-mRNA-1
chr2_1 CDS 5144036 5144218 5144036 ID=Msa0189680-mRNA-1:cds;Parent=Msa0189680-mRNA-1
chr2_1 CDS 5144356 5144485 5144356 ID=Msa0189680-mRNA-1:cds;Parent=Msa0189680-mRNA-1
chr2_1 CDS 5145539 5145957 5145539 ID=Msa0189680-mRNA-1:cds;Parent=Msa0189680-mRNA-1
Gene Sequence

>Msa0189680

ATGGCTTACTTTGTTTTTTTGTCTCTCTTATTCTTATTCTTAATCTTAATTGATGGTTCTAAGAGTCAGCTTCATGTTGGTTTCTACTCCAACACATGTCCTCAAGTAGAGTCCACTGTCCATGATGTTGTTAGAGAAGCTGTTCTTTCTGACAGAACCATGGCTGCTGTTCTTCTCAGGCTTCATTTTCATGACTGCTTTGTTGAGGGATGTGATGGTTCAATACTGATTAATACTACTCAAAATCCAGAGAAGACTGCATCTCCACATGCAGGTGTTAGAGGATATGATGTGATAGAAAAAGCCAAGGCACAATTAGAAGCATCTTGTCCAGGAGTGGTTTCTTGTGCAGATATTGTTGCTTTGGCAGCAAGAGATGCAATTGTCATGGCAAATGGACCTGCATATCAAGTTCCAACAGGGAGAAGAGATGGATTGGTTTCAGATAAATCTCTTGCAGATAATATGCCGGATGTTGATGATTCAATTCAGCAACTCAAGGCCAAGTTTCTGAACAAGGCTGCTCATACAATTGGAACCACAGCATGCTTCTTTATGAGAAAAAGACTATACGAATTTTTCCCATTTGGATCAGACCCAACTATTAACCTGAATTTCCTCCCAGAACTGAAAGCAAGGTGCCCTAAGGATGGAGATATCAACAAACGTTTAGCCATGGATGAAGGGAGTGATCTGAAATTTGACAAGAGTATATTGAAAAACATAAGGGAAGGTTTTGCTGTGTTAGCTTCTGATGCAAGACTTAATGATGACTTTGTAACAAAGAGTGTCATTGATTCCTACTTTAATCCTATAAATCCAACATTTGGACCTTCTTTTGAAAATGATTTTGTTCAGTCAATGGTAAAAATGGGACAAATTGGAGTAAAGACAGGTTCTGTTGGAAACATTAGGCGTGTGTGCTCAGCTTTTAATTGA
Protein sequence

>Msa0189680

MAYFVFLSLLFLFLILIDGSKSQLHVGFYSNTCPQVESTVHDVVREAVLSDRTMAAVLLRLHFHDCFVEGCDGSILINTTQNPEKTASPHAGVRGYDVIEKAKAQLEASCPGVVSCADIVALAARDAIVMANGPAYQVPTGRRDGLVSDKSLADNMPDVDDSIQQLKAKFLNKAAHTIGTTACFFMRKRLYEFFPFGSDPTINLNFLPELKARCPKDGDINKRLAMDEGSDLKFDKSILKNIREGFAVLASDARLNDDFVTKSVIDSYFNPINPTFGPSFENDFVQSMVKMGQIGVKTGSVGNIRRVCSAFN*