AlfalfaGEDB | Msa0800120

M. sativa cultivar zhongmu-4 / Msa0800120

KEGG: -
GO: GO:0000003 GO:0001101 GO:0003002 GO:0003006 GO:0003674 GO:0003824 GO:0004672 GO:0004888 GO:0005034 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0007275 GO:0007389 GO:0008150 GO:0008152 GO:0009414 GO:0009415 GO:0009628 GO:0009791 GO:0009927 GO:0009987 GO:0010035 GO:0010154 GO:0010375 GO:0010431 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0021700 GO:0022414 GO:0023052 GO:0032501 GO:0032502 GO:0032504 GO:0036211 GO:0038023 GO:0042221 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0048316 GO:0048608 GO:0048609 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060089 GO:0061458 GO:0065007 GO:0071695 GO:0071704 GO:0071944 GO:0140096 GO:1901564 GO:1901700
PFAM: PF00512.28

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0800120	sp\|Q9SXL4\|AHK1_ARATH	65.414	1197	368	14	4	1177	26	1199	0.0	1575

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0800120	G7JXI9	97.451	1177	28	2	1	1177	25	1199	0.0	2394

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TFs/TRs:

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Gene ID	Type	Classification
Msa0800120	TR	Others

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0002110	Msa0800120	-0.823058	1.698880e-53	-8.615850e-47
Msa0003840	Msa0800120	0.800032	1.743196e-48	-8.615850e-47
Msa0004180	Msa0800120	-0.825470	4.607208e-54	-8.615850e-47
Msa0004940	Msa0800120	0.803761	2.987204e-49	-8.615850e-47
Msa0006080	Msa0800120	0.805210	1.489790e-49	-8.615850e-47
Msa0007810	Msa0800120	-0.818516	1.882341e-52	-8.615850e-47
Msa0011010	Msa0800120	0.828162	1.048100e-54	-8.615850e-47
Msa0011040	Msa0800120	0.804195	2.427323e-49	-8.615850e-47
Msa0011060	Msa0800120	-0.817023	4.089113e-52	-8.615850e-47
Msa0011100	Msa0800120	-0.815465	9.118795e-52	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0800120	MtrunA17_Chr5g0405701	97.451	1177	28	2	1	1177	25	1199	0.0	2394
Msa0800120	MtrunA17_Chr8g0371601	73.610	1205	256	14	1	1178	1	1170	0.0	1748
Msa0800120	MtrunA17_Chr1g0197591	31.429	385	236	7	385	751	280	654	1.71e-40	162
Msa0800120	MtrunA17_Chr1g0197591	38.129	139	63	5	1023	1161	921	1036	5.44e-16	83.6
Msa0800120	MtrunA17_Chr8g0377481	34.062	320	187	6	439	741	345	657	3.77e-38	155
Msa0800120	MtrunA17_Chr8g0377481	34.722	144	62	2	1025	1162	838	955	6.03e-18	90.1
Msa0800120	MtrunA17_Chr1g0194741	33.429	347	209	8	398	725	307	650	5.78e-38	152
Msa0800120	MtrunA17_Chr3g0122391	32.704	318	192	6	442	741	396	709	9.18e-38	154
Msa0800120	MtrunA17_Chr3g0122391	34.932	146	64	2	1022	1162	893	1012	9.58e-17	86.3
Msa0800120	MtrunA17_Chr8g0392301	33.036	336	191	7	439	741	336	670	7.62e-36	147

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0800120	AT2G17820.1	65.414	1197	368	14	4	1177	26	1199	0.0	1575
Msa0800120	AT2G47430.1	28.793	837	466	25	410	1162	318	1108	7.82e-73	265
Msa0800120	AT1G27320.1	34.036	332	209	4	436	758	400	730	1.24e-41	167
Msa0800120	AT1G27320.1	32.051	156	62	2	1025	1162	887	1016	8.06e-15	80.1
Msa0800120	AT2G01830.2	32.727	330	195	6	445	749	431	758	1.96e-41	166
Msa0800120	AT2G01830.2	33.540	161	75	2	1022	1176	939	1073	6.94e-17	87.0
Msa0800120	AT2G01830.1	33.230	322	188	6	445	741	408	727	2.02e-41	166
Msa0800120	AT2G01830.1	33.540	161	75	2	1022	1176	916	1050	8.13e-17	86.7
Msa0800120	AT2G01830.4	33.230	322	188	6	445	741	408	727	2.02e-41	166
Msa0800120	AT2G01830.4	33.540	161	75	2	1022	1176	916	1050	8.13e-17	86.7

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Find 194 sgRNAs with CRISPR-Local

Find 366 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGCAATAAGGAAATCAG+AGG	0.621819	5_3:+13535611	Msa0800120:CDS
AAAAGTATTATGGCTGTTGT+TGG	0.492363	5_3:+13530559	Msa0800120:CDS
AAACTTGAAGAGACTTCCTT+TGG	0.443045	5_3:+13530894	Msa0800120:CDS
AAAGTATATCATGGGACAGG+TGG	0.583109	5_3:+13531497	Msa0800120:CDS
AAATAAACCTCATGTTTGCA+AGG	0.556476	5_3:-13531707	None:intergenic
AAATTAGTTAGAGGTGACTC+TGG	0.577940	5_3:+13532679	Msa0800120:CDS
AACACAGAATCCCATTTGCT+TGG	0.417471	5_3:-13533223	None:intergenic
AACCGATGAAAATGAACATT+TGG	0.389298	5_3:+13534768	Msa0800120:CDS
AACTTCCTTCACAAGTATTT+CGG	0.341250	5_3:+13534457	Msa0800120:CDS
AAGAAAAGTAACTCACCAAG+AGG	0.687749	5_3:+13528968	Msa0800120:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CTTTATGTTCATCAAGTTCA+AGG	+	chr5_3:13528882-13528901	Msa0800120:five_prime_UTR	30.0%
TACCTATATTTATTGCCCAT+TGG	-	chr5_3:13528911-13528930	None:intergenic	30.0%
TAATGTTAGCCATCTTGATA+GGG	+	chr5_3:13529236-13529255	Msa0800120:CDS	30.0%
TGTGAAATGCCATGTTAATA+TGG	-	chr5_3:13529269-13529288	None:intergenic	30.0%
ATATTCCACATCCTCAAAAT+CGG	-	chr5_3:13529358-13529377	None:intergenic	30.0%
TAAGTCAGTTATGGCTAAAT+TGG	+	chr5_3:13529556-13529575	Msa0800120:intron	30.0%
AGGTTGAATTGATGAAATTC+AGG	+	chr5_3:13529988-13530007	Msa0800120:intron	30.0%
GGTTGAATTGATGAAATTCA+GGG	+	chr5_3:13529989-13530008	Msa0800120:intron	30.0%
AGGACATCCATAACAAAAAT+TGG	-	chr5_3:13530075-13530094	None:intergenic	30.0%
AGATCTTTCAAATTACTCCA+TGG	+	chr5_3:13530255-13530274	Msa0800120:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_3	gene	13528831	13535988	13528831	ID=Msa0800120;Name=Msa0800120
chr5_3	mRNA	13528831	13535988	13528831	ID=Msa0800120-mRNA-1;Parent=Msa0800120;Name=Msa0800120-mRNA-1;_AED=0.08;_eAED=0.08;_QI=80\|0.91\|0.92\|1\|1\|1\|13\|191\|1178
chr5_3	exon	13528831	13529096	13528831	ID=Msa0800120-mRNA-1:exon:5570;Parent=Msa0800120-mRNA-1
chr5_3	exon	13529235	13529477	13529235	ID=Msa0800120-mRNA-1:exon:5571;Parent=Msa0800120-mRNA-1
chr5_3	exon	13530109	13530915	13530109	ID=Msa0800120-mRNA-1:exon:5572;Parent=Msa0800120-mRNA-1
chr5_3	exon	13531474	13531721	13531474	ID=Msa0800120-mRNA-1:exon:5573;Parent=Msa0800120-mRNA-1
chr5_3	exon	13531987	13532146	13531987	ID=Msa0800120-mRNA-1:exon:5574;Parent=Msa0800120-mRNA-1
chr5_3	exon	13532267	13532393	13532267	ID=Msa0800120-mRNA-1:exon:5575;Parent=Msa0800120-mRNA-1
chr5_3	exon	13532668	13532757	13532668	ID=Msa0800120-mRNA-1:exon:5576;Parent=Msa0800120-mRNA-1
chr5_3	exon	13532917	13533111	13532917	ID=Msa0800120-mRNA-1:exon:5577;Parent=Msa0800120-mRNA-1
chr5_3	exon	13533215	13533284	13533215	ID=Msa0800120-mRNA-1:exon:5578;Parent=Msa0800120-mRNA-1
chr5_3	exon	13533568	13533610	13533568	ID=Msa0800120-mRNA-1:exon:5579;Parent=Msa0800120-mRNA-1
chr5_3	exon	13533882	13534022	13533882	ID=Msa0800120-mRNA-1:exon:5580;Parent=Msa0800120-mRNA-1
chr5_3	exon	13534157	13535167	13534157	ID=Msa0800120-mRNA-1:exon:5581;Parent=Msa0800120-mRNA-1
chr5_3	exon	13535582	13535988	13535582	ID=Msa0800120-mRNA-1:exon:5582;Parent=Msa0800120-mRNA-1
chr5_3	five_prime_UTR	13528831	13528910	13528831	ID=Msa0800120-mRNA-1:five_prime_utr;Parent=Msa0800120-mRNA-1
chr5_3	CDS	13528911	13529096	13528911	ID=Msa0800120-mRNA-1:cds;Parent=Msa0800120-mRNA-1
chr5_3	CDS	13529235	13529477	13529235	ID=Msa0800120-mRNA-1:cds;Parent=Msa0800120-mRNA-1
chr5_3	CDS	13530109	13530915	13530109	ID=Msa0800120-mRNA-1:cds;Parent=Msa0800120-mRNA-1
chr5_3	CDS	13531474	13531721	13531474	ID=Msa0800120-mRNA-1:cds;Parent=Msa0800120-mRNA-1
chr5_3	CDS	13531987	13532146	13531987	ID=Msa0800120-mRNA-1:cds;Parent=Msa0800120-mRNA-1
chr5_3	CDS	13532267	13532393	13532267	ID=Msa0800120-mRNA-1:cds;Parent=Msa0800120-mRNA-1
chr5_3	CDS	13532668	13532757	13532668	ID=Msa0800120-mRNA-1:cds;Parent=Msa0800120-mRNA-1
chr5_3	CDS	13532917	13533111	13532917	ID=Msa0800120-mRNA-1:cds;Parent=Msa0800120-mRNA-1
chr5_3	CDS	13533215	13533284	13533215	ID=Msa0800120-mRNA-1:cds;Parent=Msa0800120-mRNA-1
chr5_3	CDS	13533568	13533610	13533568	ID=Msa0800120-mRNA-1:cds;Parent=Msa0800120-mRNA-1
chr5_3	CDS	13533882	13534022	13533882	ID=Msa0800120-mRNA-1:cds;Parent=Msa0800120-mRNA-1
chr5_3	CDS	13534157	13535167	13534157	ID=Msa0800120-mRNA-1:cds;Parent=Msa0800120-mRNA-1
chr5_3	CDS	13535582	13535797	13535582	ID=Msa0800120-mRNA-1:cds;Parent=Msa0800120-mRNA-1
chr5_3	three_prime_UTR	13535798	13535988	13535798	ID=Msa0800120-mRNA-1:three_prime_utr;Parent=Msa0800120-mRNA-1

Gene Sequence

>Msa0800120

ATGGGCAATAAATATAGGTACATGTTTAATAGACTATTCAGGTTTACCAATTCATGGAAGAAAAGTAACTCACCAAGAGGTAGAAGAATTTTCCACAGAGATGTTGAAAAAGAAGAATTCCAATATGCTAGTAGTCACTGTCTTTCATCCTACTACAGTGTTTTTGTGGTTAGATTAGCAATCATGGTAATGTTAGCCATCTTGATAGGGCTATTGACCATATTAACATGGCATTTCACAAAGATTTATACGAAAAAATCATTGAGTAGTTTGGCGTATGGTCTGCGTTATGAACTTCTGCAACGTCCGATTTTGAGGATGTGGAATATTTTGAATTCTACTTCTGAAATTACAACAGCTCAGGTCAAGTTGTCTCAATATGTGATCAGACGTTATAGCAATCCTGCATCTCAAGCTGAGCAAGTTGAGCAGCTATATGAAGCAATGAGGGCAGTAACATGGTCACTATTTGCTAGTAGAAAAGCACTAAACTCAATAACAATCAATTACAAGAATGGATTTGTTCAAGCATTCCATAGAGATCTAAAAGATAACAACACATTTTACATCTACTCAGATCTTTCAAATTACTCCATGGTTGCATCAACCTCAAACATGGTCAAGTCCCTTTCAACTCATCAAGCTTGGGATGACAAAACAATTCATGGTAACTTTTCAGCAATTTGGTATAGAGAACCACTTGATCCTGTCACAGGCGAAAAAATCGGAAAAGCAATGAAAATTGCACCAGAAGATTTGATCAACATAGCAGGACTTTCACAAGTTCCGGATGGTGTAGCTACATGGCATGTAGCAGTTAGTAAATTTACAGATTCGCCTTTGCTTTCGGCAGCTTTACCTGTTTGGGATTCTTCTAATAAAAGTATTATGGCTGTTGTTGGTGTTACAACTGCATTTTATAGTGTTGGACAGTTAATGAGAGAGTTAGTTGAGATGCATAGTGGTCACATGTATTTGACTTCTCAACAAGGCTATTTACTTGCAACTTCTACTAGTGCACCGTTACTTACAAATTCGACTAAGCCACCACCAAAGCTTAAGATGGCTGTGGATTGTGAAGATGATATAATAAGACTTGGAGCTGAGTGGTTGCAGAGAACTTATGGGAATCATTTTCCTAATTCAACTCGTGAGGTTCATGTTGAGAATGCTAAGCTAGGGAATCAGCAATATTATATTGACTCGTTCTACCTAAACTTGAAGAGACTTCCTTTGGTAGGTGTAATCATCATACCAAGAAAGTATATCATGGGACAGGTGGATGAGAGATCTTTCAAAACATTGGTTATTCTGATATCTGCATCTTTATGCATTTTTGTCATTGGATGTGTTTGCATTTTGATATTGACAAATGGAGTGTCAAAGGAAATGAATCTGAGAGCAGAACTGATAAGTCAACTTGAAGCAAGAAGGAAGGCAGAGGCATCAAGCAACTATAAAAGTCAATTCCTTGCAAACATGAGTCATGAACTAAGGACACCTATGGCAGCAGTAATTGGATTGCTTGACATTCTTATATCTGATGATTGTCTCACAAATGAACAATATTCAACAGTTACTCAAATAAGAAAATGTTCAACAGCTCTTCTCCGTCTTCTTAACAACATATTGGATCTGAGCAAGGTGGAGTCTGGAAAACTTGTACTAGAAGATGCAGAATTCGACTTGGGACGCGAACTTGAAGGGCTTGTAGATATGTTTTCTGTTCAGTGCATTAATCACAATGTAGAGACTGTTTTAGACCTTTCTGATGATATGCCAAAATTAGTTAGAGGTGACTCTGGCAGGGTGGTTCAAGTATTTGCAAATTTGATCAACAATTCAATTAAGTTTACTACATCTGGTCATGTTATTCTGCGAGGATGGTGTGAATATCCAAACTCTTGCAACGATAGTCCGAATTTTTATCTTGATCAGAAGAAATCAAGAAATTTACATAAGAGCAAAGAGAAGCCAAATGCAAGTCATGCAAAGAGAATATCTATGAGAGATAACAAAGTGATACTTTGGTTTGAAGTTGAGGACACAGGCTGTGGTATCGATCCAAGCAAATGGGATTCTGTGTTTGAAAGCTTTGAGCAAGCTGATCCATCAACTACTAGACTGCATGGAGGGACTGGTCTAGGTCTTTGCATCGTTAGGAACTTGGTTAATAAGATGGGTGGAGAAATCAAGGTTACCAAAAAGGAGGGTCAAGGAACATTGATGAGATTATGTTTGCTTCTTAGTGTACCAATGGATGTCACAGAACAACATAGTGCTTTAAATTTGACAGACAATGGCTTAGTGGTATTGCTTGCACTACAAGGCAATATGGGTAGAATAATAACATCGAAGTGGTTAACCAAAAAAGGGGTGTGCACAATGGAAGCATCTGAATGGAATGGACTAACACAAATTTTGAGGGAACTCTTTCATGCAAGAAGTTCAGTCCATAACAATAACAACTTTGATGCACATTATCCAATAAATCTTGAAGGATTTAAGTCTAAACTACTAAGCATCAAAGATATGAGGAATCCAATTTTTGTCATTGTTGTTGACATTGGTTTGCTTGACTTAAGCACAGATATATGGAAAGAACAACTTAACTTCCTTCACAAGTATTTCGGAAGAGCAAAATTTGTGTGGATTCTGAATCATGACACATCAAATACCATAAAGACTGAGCTTCGTCGGAAAGGACACTTACTAATGGTTAACAAACCTCTATACAAAGCAAAAATGATTCATATTTTGGAAGCTGTTATAAAAGAGAGAAATCTTGAGTTGCAAAAGAAAAACATGAAAGAGGGTGATTTGCATGAGTTTCTTGAGATTGATTCTACTCAATTTGAAGCTGCTACTAGCTCTGATGATTCAGATATATCTGAAATTAGTGAAAAACCAATTTTAAAGTTAACCGATGAAAATGAACATTTGGAAGAAAATAATCCAAGGGAAGAAGAATCTTGTACCTCTGAAGACAACAAAGCTAAAGAAACAAACTTTGCAACACAAGCTTATGATAGGGTGACAAGGTCAAGAAAAGAAGTGAATGATAAGAAATCACTTGAAGGGTTAAGGATTTTGCTTGCAGAAGATACACCAGTAATTCAAAGGGTTGCAACAATAATGCTTGAAAAAATGGGAGCTGTTGTTGTTGCTGTAGGTGATGGACAACAAGCAGTGGATGCTCTTAATTATATGGTAAGTGCTGAAGATTGTAGAAGAGAGTCTCTCCAGAAGGACAAAAATACAAGATCACAAACAGAAATTTTGACTTGCCATCAATATGATTTGATCCTAATGGATTGTCAGATGCCAAAGATGGATGGATATGAGGCAACAAAAGCAATAAGGAAATCAGAGGAAGGAACAGGCAAACACATTCCAATTGTTGCTCTAACAGCCCATGCAATGTCTTGTGATGAAGCCAAATGCCTTAAGGTTGGCATGGATGCTTACTTAACAAAGCCAATTGACTTCAAGCTTATGGTCTCAACCATTCTCTCACTCACTAAACCAACAACCTAG

Protein sequence

>Msa0800120

MGNKYRYMFNRLFRFTNSWKKSNSPRGRRIFHRDVEKEEFQYASSHCLSSYYSVFVVRLAIMVMLAILIGLLTILTWHFTKIYTKKSLSSLAYGLRYELLQRPILRMWNILNSTSEITTAQVKLSQYVIRRYSNPASQAEQVEQLYEAMRAVTWSLFASRKALNSITINYKNGFVQAFHRDLKDNNTFYIYSDLSNYSMVASTSNMVKSLSTHQAWDDKTIHGNFSAIWYREPLDPVTGEKIGKAMKIAPEDLINIAGLSQVPDGVATWHVAVSKFTDSPLLSAALPVWDSSNKSIMAVVGVTTAFYSVGQLMRELVEMHSGHMYLTSQQGYLLATSTSAPLLTNSTKPPPKLKMAVDCEDDIIRLGAEWLQRTYGNHFPNSTREVHVENAKLGNQQYYIDSFYLNLKRLPLVGVIIIPRKYIMGQVDERSFKTLVILISASLCIFVIGCVCILILTNGVSKEMNLRAELISQLEARRKAEASSNYKSQFLANMSHELRTPMAAVIGLLDILISDDCLTNEQYSTVTQIRKCSTALLRLLNNILDLSKVESGKLVLEDAEFDLGRELEGLVDMFSVQCINHNVETVLDLSDDMPKLVRGDSGRVVQVFANLINNSIKFTTSGHVILRGWCEYPNSCNDSPNFYLDQKKSRNLHKSKEKPNASHAKRISMRDNKVILWFEVEDTGCGIDPSKWDSVFESFEQADPSTTRLHGGTGLGLCIVRNLVNKMGGEIKVTKKEGQGTLMRLCLLLSVPMDVTEQHSALNLTDNGLVVLLALQGNMGRIITSKWLTKKGVCTMEASEWNGLTQILRELFHARSSVHNNNNFDAHYPINLEGFKSKLLSIKDMRNPIFVIVVDIGLLDLSTDIWKEQLNFLHKYFGRAKFVWILNHDTSNTIKTELRRKGHLLMVNKPLYKAKMIHILEAVIKERNLELQKKNMKEGDLHEFLEIDSTQFEAATSSDDSDISEISEKPILKLTDENEHLEENNPREEESCTSEDNKAKETNFATQAYDRVTRSRKEVNDKKSLEGLRILLAEDTPVIQRVATIMLEKMGAVVVAVGDGQQAVDALNYMVSAEDCRRESLQKDKNTRSQTEILTCHQYDLILMDCQMPKMDGYEATKAIRKSEEGTGKHIPIVALTAHAMSCDEAKCLKVGMDAYLTKPIDFKLMVSTILSLTKPTT*