AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0192490

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0192490 MtrunA17_Chr2g0282671 97.647 85 2 0 1 85 2 86 3.24e-56 168
Msa0192490 MtrunA17_Chr4g0040461 49.425 87 41 2 2 85 4 90 1.91e-17 70.5
Msa0192490 MtrunA17_Chr2g0324851 55.357 56 25 0 30 85 36 91 5.35e-15 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0192490 AT1G28395.2 59.211 76 27 2 17 90 22 95 5.64e-23 84.7
Msa0192490 AT1G28395.3 59.211 76 27 2 17 90 22 95 5.64e-23 84.7
Msa0192490 AT1G28395.1 59.211 76 27 2 17 90 22 95 5.64e-23 84.7
Msa0192490 AT2G33847.2 58.427 89 32 3 1 85 1 88 3.39e-22 82.8
Msa0192490 AT2G33847.1 59.302 86 30 3 1 82 1 85 2.29e-21 80.5
Msa0192490 AT1G28395.6 59.155 71 25 2 17 85 22 90 2.27e-20 78.2
Msa0192490 AT1G28395.4 59.155 71 25 2 17 85 22 90 2.27e-20 78.2
Msa0192490 AT1G28395.5 62.687 67 21 2 17 81 22 86 3.34e-20 77.8
Msa0192490 AT2G20585.4 66.667 42 14 0 44 85 54 95 6.56e-13 59.3
Msa0192490 AT2G20585.3 66.667 42 14 0 44 85 54 95 6.56e-13 59.3
Msa0192490 AT2G20585.6 66.667 42 14 0 44 85 54 95 1.04e-12 58.9
Msa0192490 AT2G20585.7 66.667 42 14 0 44 85 54 95 1.04e-12 58.9
Msa0192490 AT2G20585.5 71.053 38 11 0 44 81 54 91 4.32e-12 57.4
Msa0192490 AT2G20585.1 71.053 38 11 0 44 81 54 91 4.32e-12 57.4
Msa0192490 AT2G20585.2 71.053 38 11 0 44 81 54 91 4.51e-12 57.4

Find 53 sgRNAs with CRISPR-Local

Find 288 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTAAATTGAATGTAAAAT+TGG 0.157129 2_1:-8319577 Msa0192490:three_prime_UTR
AATCCATAATGGATCTTATA+AGG 0.223730 2_1:+8319384 None:intergenic
CTCCCTTATAAGATCCATTA+TGG 0.228413 2_1:-8319387 Msa0192490:three_prime_UTR
TTGGAAATTGAGGTTTGTTT+TGG 0.242742 2_1:+8321083 None:intergenic
CGCCGCTTCTCGCGCCAACC+TGG 0.287298 2_1:-8321150 Msa0192490:CDS
TACTGACAAATTATTGTGAT+TGG 0.300576 2_1:+8319481 None:intergenic
CCTCCTTCCCAAAACCCTAT+AGG 0.369977 2_1:-8321260 Msa0192490:five_prime_UTR
GACCTGCATGCTCAATGTTT+CGG 0.399809 2_1:-8321109 Msa0192490:CDS
TCTCGCGCCAACCTGGCTGC+CGG 0.401245 2_1:-8321143 Msa0192490:CDS
ATCTGGCGGCTGACATCGTT+AGG 0.403500 2_1:+8321186 None:intergenic
CTAAATAACACAATCCATAA+TGG 0.406316 2_1:+8319373 None:intergenic
GAAGCTGTTGCAGTGTGATA+TGG 0.410643 2_1:+8320923 None:intergenic
GGGACGAACACCGGAGGTTA+TGG 0.417357 2_1:+8321207 None:intergenic
GTGGCTATGGTGGAATTTAA+TGG 0.421081 2_1:-8319521 Msa0192490:three_prime_UTR
CAATTTCCAATCGCATTTCA+AGG 0.423789 2_1:-8321070 Msa0192490:intron
TAGGTTTGGCTCCGGCAGCC+AGG 0.427645 2_1:+8321132 None:intergenic
TTTGGCTCCGGCAGCCAGGT+TGG 0.437067 2_1:+8321136 None:intergenic
AAGGAAGGAGACAGAGGGAG+AGG 0.437111 2_1:+8319410 None:intergenic
TTGCCAGTGGAGATGAGTTC+CGG 0.437830 2_1:-8320962 Msa0192490:CDS
ACTGACAAATTATTGTGATT+GGG 0.441848 2_1:+8319482 None:intergenic
TCAGTTTCTCGCCACTCTTA+CGG 0.444023 2_1:-8320878 Msa0192490:CDS
TGAGCATGCAGGTCTAGGTT+TGG 0.456182 2_1:+8321118 None:intergenic
AGCCAGGTTGGCGCGAGAAG+CGG 0.458390 2_1:+8321148 None:intergenic
ATCCATAATGGATCTTATAA+GGG 0.461466 2_1:+8319385 None:intergenic
AACATTGAGCATGCAGGTCT+AGG 0.474408 2_1:+8321113 None:intergenic
TTCATTCTCTCGCTAATCTA+CGG 0.482308 2_1:+8319602 None:intergenic
GGCGGAGCGAAGGAAGCATC+TGG 0.487477 2_1:+8321169 None:intergenic
TGAAATGCGATTGGAAATTG+AGG 0.488069 2_1:+8321073 None:intergenic
TTTCTCGCCACTCTTACGGC+TGG 0.489269 2_1:-8320874 Msa0192490:CDS
CGCGAGAAGCGGCGGAGCGA+AGG 0.493060 2_1:+8321159 None:intergenic
TGCAGGTCTAGGTTTGGCTC+CGG 0.513928 2_1:+8321124 None:intergenic
TTGTTACCTTGAAATGCGAT+TGG 0.518636 2_1:+8321064 None:intergenic
CAGGTTGGCGCGAGAAGCGG+CGG 0.521433 2_1:+8321151 None:intergenic
AGGGAGAAGGAAGGAGACAG+AGG 0.535539 2_1:+8319404 None:intergenic
GGGAGAAGGAAGGAGACAGA+GGG 0.548350 2_1:+8319405 None:intergenic
ATCCGAAACATTGAGCATGC+AGG 0.553491 2_1:+8321107 None:intergenic
GATCTTATAAGGGAGAAGGA+AGG 0.558040 2_1:+8319395 None:intergenic
ACGCCGGAACTCATCTCCAC+TGG 0.567030 2_1:+8320959 None:intergenic
AAACTGATGTGTGGCTATGG+TGG 0.567218 2_1:-8319531 Msa0192490:three_prime_UTR
AATGGATCTTATAAGGGAGA+AGG 0.574760 2_1:+8319391 None:intergenic
ACGAACACCGGAGGTTATGG+TGG 0.576192 2_1:+8321210 None:intergenic
GCATCAAAGATGGTGTGACA+TGG 0.577104 2_1:-8319669 Msa0192490:three_prime_UTR
CCACTCTTACGGCTGGACTC+CGG 0.581950 2_1:-8320867 Msa0192490:CDS
TGAAGAGCGAGCATCAAAGA+TGG 0.593981 2_1:-8319679 Msa0192490:three_prime_UTR
CCGGAGTCCAGCCGTAAGAG+TGG 0.594748 2_1:+8320867 None:intergenic
CGAACACCGGAGGTTATGGT+GGG 0.604922 2_1:+8321211 None:intergenic
TCTGGCGGCTGACATCGTTA+GGG 0.611140 2_1:+8321187 None:intergenic
TTTGTTGTGTAGATTGCCAG+TGG 0.631697 2_1:-8320975 Msa0192490:intron
GGAGCGAAGGAAGCATCTGG+CGG 0.642459 2_1:+8321172 None:intergenic
TCGTTAGGGACGAACACCGG+AGG 0.667385 2_1:+8321201 None:intergenic
TCTTACGGCTGGACTCCGGA+AGG 0.672791 2_1:-8320863 Msa0192490:intron
ACATCGTTAGGGACGAACAC+CGG 0.677471 2_1:+8321198 None:intergenic
TGGAAGCAAAGATTCGACGC+CGG 0.708954 2_1:+8320943 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATAAAGTCAAATTGTTT+CGG + chr2_1:8320338-8320357 None:intergenic 15.0%
!! TTCATTATTAAGTAATAAAG+AGG + chr2_1:8320522-8320541 None:intergenic 15.0%
!!! GTATCAAAAATTCATTTTTA+CGG + chr2_1:8320568-8320587 None:intergenic 15.0%
!! ATTTATATCAATACGAAAAA+AGG + chr2_1:8320679-8320698 None:intergenic 15.0%
!!! ATGGATTTATTTCAAATTTT+AGG - chr2_1:8320960-8320979 Msa0192490:CDS 15.0%
!! TTCTAAATTGAATGTAAAAT+TGG - chr2_1:8321033-8321052 Msa0192490:intron 15.0%
!! AAAATAAAGTCAAATTGTTT+CGG + chr2_1:8320338-8320357 None:intergenic 15.0%
!! TTCATTATTAAGTAATAAAG+AGG + chr2_1:8320522-8320541 None:intergenic 15.0%
!!! GTATCAAAAATTCATTTTTA+CGG + chr2_1:8320568-8320587 None:intergenic 15.0%
!! ATTTATATCAATACGAAAAA+AGG + chr2_1:8320679-8320698 None:intergenic 15.0%
!!! ATGGATTTATTTCAAATTTT+AGG - chr2_1:8320960-8320979 Msa0192490:CDS 15.0%
!! TTCTAAATTGAATGTAAAAT+TGG - chr2_1:8321033-8321052 Msa0192490:intron 15.0%
!!! TTCTTTTTTTGATTCCTATA+GGG + chr2_1:8319367-8319386 None:intergenic 20.0%
!! AATTCGAGTAAACTATTATT+AGG + chr2_1:8319593-8319612 None:intergenic 20.0%
!! TGATAGTTAAACAATAATTG+AGG + chr2_1:8319788-8319807 None:intergenic 20.0%
!!! TTATAATTGCTTTTTTCAAG+TGG - chr2_1:8319835-8319854 Msa0192490:intron 20.0%
!!! TATAATTGCTTTTTTCAAGT+GGG - chr2_1:8319836-8319855 Msa0192490:intron 20.0%
!! AATTTATCAAATGCTGAAAT+TGG - chr2_1:8319895-8319914 Msa0192490:intron 20.0%
!! TATTATTCATAATCTTGTCA+AGG + chr2_1:8320160-8320179 None:intergenic 20.0%
!!! CTTTATTTTATCTTGTGTTA+TGG - chr2_1:8320349-8320368 Msa0192490:intron 20.0%
!! AAATAAGTTGTTTATCCAAA+CGG - chr2_1:8320410-8320429 Msa0192490:intron 20.0%
!! GATATAAATATTAACCTTGT+TGG - chr2_1:8320690-8320709 Msa0192490:intron 20.0%
!! AAAAGTAAAACAAACAAAGA+TGG + chr2_1:8320718-8320737 None:intergenic 20.0%
!!! TTTTTCAATTGTACCTTTTT+TGG - chr2_1:8320734-8320753 Msa0192490:intron 20.0%
!! GTTTGAACAATAAAAATAGT+TGG + chr2_1:8320814-8320833 None:intergenic 20.0%
!!! AACTTTTTTTCCTTTGAATT+AGG - chr2_1:8320840-8320859 Msa0192490:intron 20.0%
!!! ACTTTTTTTCCTTTGAATTA+GGG - chr2_1:8320841-8320860 Msa0192490:intron 20.0%
!!! TTCTTTTTTTGATTCCTATA+GGG + chr2_1:8319367-8319386 None:intergenic 20.0%
!! AATTCGAGTAAACTATTATT+AGG + chr2_1:8319593-8319612 None:intergenic 20.0%
!! TGATAGTTAAACAATAATTG+AGG + chr2_1:8319788-8319807 None:intergenic 20.0%
!!! TTATAATTGCTTTTTTCAAG+TGG - chr2_1:8319835-8319854 Msa0192490:intron 20.0%
!!! TATAATTGCTTTTTTCAAGT+GGG - chr2_1:8319836-8319855 Msa0192490:intron 20.0%
!! AATTTATCAAATGCTGAAAT+TGG - chr2_1:8319895-8319914 Msa0192490:intron 20.0%
!! TATTATTCATAATCTTGTCA+AGG + chr2_1:8320160-8320179 None:intergenic 20.0%
!!! CTTTATTTTATCTTGTGTTA+TGG - chr2_1:8320349-8320368 Msa0192490:intron 20.0%
!! AAATAAGTTGTTTATCCAAA+CGG - chr2_1:8320410-8320429 Msa0192490:intron 20.0%
!! GATATAAATATTAACCTTGT+TGG - chr2_1:8320690-8320709 Msa0192490:intron 20.0%
!! AAAAGTAAAACAAACAAAGA+TGG + chr2_1:8320718-8320737 None:intergenic 20.0%
!!! TTTTTCAATTGTACCTTTTT+TGG - chr2_1:8320734-8320753 Msa0192490:intron 20.0%
!! GTTTGAACAATAAAAATAGT+TGG + chr2_1:8320814-8320833 None:intergenic 20.0%
!!! AACTTTTTTTCCTTTGAATT+AGG - chr2_1:8320840-8320859 Msa0192490:intron 20.0%
!!! ACTTTTTTTCCTTTGAATTA+GGG - chr2_1:8320841-8320860 Msa0192490:intron 20.0%
!!! CTTCTTTTTTTGATTCCTAT+AGG + chr2_1:8319368-8319387 None:intergenic 25.0%
! AAGTTTGTATAGACTTTACA+TGG - chr2_1:8319947-8319966 Msa0192490:intron 25.0%
! ACTTATTTATTTGCATAAGC+TGG + chr2_1:8320069-8320088 None:intergenic 25.0%
! TGTGTTAATTCATAAGTTCT+CGG - chr2_1:8320092-8320111 Msa0192490:intron 25.0%
! GTTAATGAAAAAACAGCTTA+TGG + chr2_1:8320135-8320154 None:intergenic 25.0%
! AATAAGTTGTTTATCCAAAC+GGG - chr2_1:8320411-8320430 Msa0192490:intron 25.0%
! TAAATCTTCTACCAAATGAA+GGG + chr2_1:8320462-8320481 None:intergenic 25.0%
!! AAGATTTAGTGTCTAGAATA+TGG - chr2_1:8320474-8320493 Msa0192490:intron 25.0%
!! AGATTTAGTGTCTAGAATAT+GGG - chr2_1:8320475-8320494 Msa0192490:intron 25.0%
!!! TACGTTTTTTATCCTCTTAT+TGG - chr2_1:8320647-8320666 Msa0192490:intron 25.0%
! ACTGACAAATTATTGTGATT+GGG + chr2_1:8321131-8321150 None:intergenic 25.0%
! TACTGACAAATTATTGTGAT+TGG + chr2_1:8321132-8321151 None:intergenic 25.0%
!!! ATCCATAATGGATCTTATAA+GGG + chr2_1:8321228-8321247 None:intergenic 25.0%
!!! AATCCATAATGGATCTTATA+AGG + chr2_1:8321229-8321248 None:intergenic 25.0%
! CTAAATAACACAATCCATAA+TGG + chr2_1:8321240-8321259 None:intergenic 25.0%
!!! CTTCTTTTTTTGATTCCTAT+AGG + chr2_1:8319368-8319387 None:intergenic 25.0%
! AAGTTTGTATAGACTTTACA+TGG - chr2_1:8319947-8319966 Msa0192490:intron 25.0%
! ACTTATTTATTTGCATAAGC+TGG + chr2_1:8320069-8320088 None:intergenic 25.0%
! TGTGTTAATTCATAAGTTCT+CGG - chr2_1:8320092-8320111 Msa0192490:intron 25.0%
! GTTAATGAAAAAACAGCTTA+TGG + chr2_1:8320135-8320154 None:intergenic 25.0%
! AATAAGTTGTTTATCCAAAC+GGG - chr2_1:8320411-8320430 Msa0192490:intron 25.0%
! TAAATCTTCTACCAAATGAA+GGG + chr2_1:8320462-8320481 None:intergenic 25.0%
!! AAGATTTAGTGTCTAGAATA+TGG - chr2_1:8320474-8320493 Msa0192490:intron 25.0%
!! AGATTTAGTGTCTAGAATAT+GGG - chr2_1:8320475-8320494 Msa0192490:intron 25.0%
!!! TACGTTTTTTATCCTCTTAT+TGG - chr2_1:8320647-8320666 Msa0192490:intron 25.0%
! ACTGACAAATTATTGTGATT+GGG + chr2_1:8321131-8321150 None:intergenic 25.0%
! TACTGACAAATTATTGTGAT+TGG + chr2_1:8321132-8321151 None:intergenic 25.0%
!!! ATCCATAATGGATCTTATAA+GGG + chr2_1:8321228-8321247 None:intergenic 25.0%
!!! AATCCATAATGGATCTTATA+AGG + chr2_1:8321229-8321248 None:intergenic 25.0%
! CTAAATAACACAATCCATAA+TGG + chr2_1:8321240-8321259 None:intergenic 25.0%
! TTTGATTCCTATAGGGTTTT+GGG + chr2_1:8319360-8319379 None:intergenic 30.0%
!! TTTTGATTCCTATAGGGTTT+TGG + chr2_1:8319361-8319380 None:intergenic 30.0%
!! TGGAAATTGAGGTTTGTTTT+GGG + chr2_1:8319529-8319548 None:intergenic 30.0%
!! TTGGAAATTGAGGTTTGTTT+TGG + chr2_1:8319530-8319549 None:intergenic 30.0%
GTTAAACAATAATTGAGGCA+AGG + chr2_1:8319783-8319802 None:intergenic 30.0%
GGAAAATAAGCTATTCCAAA+CGG + chr2_1:8320048-8320067 None:intergenic 30.0%
CAAACATAAGCCAATTCAAA+CGG - chr2_1:8320208-8320227 Msa0192490:intron 30.0%
AAACATAAGCCAATTCAAAC+GGG - chr2_1:8320209-8320228 Msa0192490:intron 30.0%
! TTTTGCAGCTTAAAGCATAA+GGG + chr2_1:8320393-8320412 None:intergenic 30.0%
! ATTTTGCAGCTTAAAGCATA+AGG + chr2_1:8320394-8320413 None:intergenic 30.0%
ATAAGTTGTTTATCCAAACG+GGG - chr2_1:8320412-8320431 Msa0192490:intron 30.0%
AAATCTTCTACCAAATGAAG+GGG + chr2_1:8320461-8320480 None:intergenic 30.0%
CTAAATCTTCTACCAAATGA+AGG + chr2_1:8320463-8320482 None:intergenic 30.0%
! GATTTAGTGTCTAGAATATG+GGG - chr2_1:8320476-8320495 Msa0192490:intron 30.0%
! ATTTAGTGTCTAGAATATGG+GGG - chr2_1:8320477-8320496 Msa0192490:intron 30.0%
!!! TTTTATCCTCTTATTGGCTT+AGG - chr2_1:8320653-8320672 Msa0192490:intron 30.0%
!!! TCAATTGTACCTTTTTTGGA+TGG - chr2_1:8320738-8320757 Msa0192490:intron 30.0%
!!! CAATTGTACCTTTTTTGGAT+GGG - chr2_1:8320739-8320758 Msa0192490:intron 30.0%
!!! AATTGTACCTTTTTTGGATG+GGG - chr2_1:8320740-8320759 Msa0192490:intron 30.0%
! TGTGTCATTGAGAAAATGTA+GGG - chr2_1:8320773-8320792 Msa0192490:intron 30.0%
ATTGAGAAAATGTAGGGTTT+GGG - chr2_1:8320779-8320798 Msa0192490:intron 30.0%
!!! TGTTATTTTTTCCCCTTTGT+AGG - chr2_1:8320892-8320911 Msa0192490:CDS 30.0%
!!! GTTATTTTTTCCCCTTTGTA+GGG - chr2_1:8320893-8320912 Msa0192490:CDS 30.0%
! ACATCAGTTTTTACGAGATA+TGG + chr2_1:8321069-8321088 None:intergenic 30.0%
! TTTGATTCCTATAGGGTTTT+GGG + chr2_1:8319360-8319379 None:intergenic 30.0%
!! TTTTGATTCCTATAGGGTTT+TGG + chr2_1:8319361-8319380 None:intergenic 30.0%
!! TGGAAATTGAGGTTTGTTTT+GGG + chr2_1:8319529-8319548 None:intergenic 30.0%
!! TTGGAAATTGAGGTTTGTTT+TGG + chr2_1:8319530-8319549 None:intergenic 30.0%
GTTAAACAATAATTGAGGCA+AGG + chr2_1:8319783-8319802 None:intergenic 30.0%
GGAAAATAAGCTATTCCAAA+CGG + chr2_1:8320048-8320067 None:intergenic 30.0%
CAAACATAAGCCAATTCAAA+CGG - chr2_1:8320208-8320227 Msa0192490:intron 30.0%
AAACATAAGCCAATTCAAAC+GGG - chr2_1:8320209-8320228 Msa0192490:intron 30.0%
! TTTTGCAGCTTAAAGCATAA+GGG + chr2_1:8320393-8320412 None:intergenic 30.0%
! ATTTTGCAGCTTAAAGCATA+AGG + chr2_1:8320394-8320413 None:intergenic 30.0%
ATAAGTTGTTTATCCAAACG+GGG - chr2_1:8320412-8320431 Msa0192490:intron 30.0%
AAATCTTCTACCAAATGAAG+GGG + chr2_1:8320461-8320480 None:intergenic 30.0%
CTAAATCTTCTACCAAATGA+AGG + chr2_1:8320463-8320482 None:intergenic 30.0%
! GATTTAGTGTCTAGAATATG+GGG - chr2_1:8320476-8320495 Msa0192490:intron 30.0%
! ATTTAGTGTCTAGAATATGG+GGG - chr2_1:8320477-8320496 Msa0192490:intron 30.0%
!!! TTTTATCCTCTTATTGGCTT+AGG - chr2_1:8320653-8320672 Msa0192490:intron 30.0%
!!! TCAATTGTACCTTTTTTGGA+TGG - chr2_1:8320738-8320757 Msa0192490:intron 30.0%
!!! CAATTGTACCTTTTTTGGAT+GGG - chr2_1:8320739-8320758 Msa0192490:intron 30.0%
!!! AATTGTACCTTTTTTGGATG+GGG - chr2_1:8320740-8320759 Msa0192490:intron 30.0%
! TGTGTCATTGAGAAAATGTA+GGG - chr2_1:8320773-8320792 Msa0192490:intron 30.0%
ATTGAGAAAATGTAGGGTTT+GGG - chr2_1:8320779-8320798 Msa0192490:intron 30.0%
!!! TGTTATTTTTTCCCCTTTGT+AGG - chr2_1:8320892-8320911 Msa0192490:CDS 30.0%
!!! GTTATTTTTTCCCCTTTGTA+GGG - chr2_1:8320893-8320912 Msa0192490:CDS 30.0%
! ACATCAGTTTTTACGAGATA+TGG + chr2_1:8321069-8321088 None:intergenic 30.0%
TGAAATGCGATTGGAAATTG+AGG + chr2_1:8319540-8319559 None:intergenic 35.0%
CAATTTCCAATCGCATTTCA+AGG - chr2_1:8319540-8319559 Msa0192490:three_prime_UTR 35.0%
TTGTTACCTTGAAATGCGAT+TGG + chr2_1:8319549-8319568 None:intergenic 35.0%
CAAGGAAATCATATACCTTC+CGG + chr2_1:8319765-8319784 None:intergenic 35.0%
TGTGACTGTTCAATTAAGCT+TGG - chr2_1:8319920-8319939 Msa0192490:intron 35.0%
AAGCACTTGTGACACTATTT+AGG - chr2_1:8320020-8320039 Msa0192490:intron 35.0%
AGCACTTGTGACACTATTTA+GGG - chr2_1:8320021-8320040 Msa0192490:intron 35.0%
AATCTTCTACCAAATGAAGG+GGG + chr2_1:8320460-8320479 None:intergenic 35.0%
! TTTAGTGTCTAGAATATGGG+GGG - chr2_1:8320478-8320497 Msa0192490:intron 35.0%
! CTGTGTCATTGAGAAAATGT+AGG - chr2_1:8320772-8320791 Msa0192490:intron 35.0%
CATTGAGAAAATGTAGGGTT+TGG - chr2_1:8320778-8320797 Msa0192490:intron 35.0%
AGAGAAAGACCCTAATTCAA+AGG + chr2_1:8320853-8320872 None:intergenic 35.0%
TTCATTCTCTCGCTAATCTA+CGG + chr2_1:8321011-8321030 None:intergenic 35.0%
ATCTCGTAAAAACTGATGTG+TGG - chr2_1:8321070-8321089 Msa0192490:intron 35.0%
TAAAAACTGATGTGTGGCTA+TGG - chr2_1:8321076-8321095 Msa0192490:CDS 35.0%
AATGGATCTTATAAGGGAGA+AGG + chr2_1:8321222-8321241 None:intergenic 35.0%
CTCCCTTATAAGATCCATTA+TGG - chr2_1:8321223-8321242 Msa0192490:five_prime_UTR 35.0%
TGAAATGCGATTGGAAATTG+AGG + chr2_1:8319540-8319559 None:intergenic 35.0%
CAATTTCCAATCGCATTTCA+AGG - chr2_1:8319540-8319559 Msa0192490:three_prime_UTR 35.0%
TTGTTACCTTGAAATGCGAT+TGG + chr2_1:8319549-8319568 None:intergenic 35.0%
CAAGGAAATCATATACCTTC+CGG + chr2_1:8319765-8319784 None:intergenic 35.0%
TGTGACTGTTCAATTAAGCT+TGG - chr2_1:8319920-8319939 Msa0192490:intron 35.0%
AAGCACTTGTGACACTATTT+AGG - chr2_1:8320020-8320039 Msa0192490:intron 35.0%
AGCACTTGTGACACTATTTA+GGG - chr2_1:8320021-8320040 Msa0192490:intron 35.0%
AATCTTCTACCAAATGAAGG+GGG + chr2_1:8320460-8320479 None:intergenic 35.0%
! TTTAGTGTCTAGAATATGGG+GGG - chr2_1:8320478-8320497 Msa0192490:intron 35.0%
! CTGTGTCATTGAGAAAATGT+AGG - chr2_1:8320772-8320791 Msa0192490:intron 35.0%
CATTGAGAAAATGTAGGGTT+TGG - chr2_1:8320778-8320797 Msa0192490:intron 35.0%
AGAGAAAGACCCTAATTCAA+AGG + chr2_1:8320853-8320872 None:intergenic 35.0%
TTCATTCTCTCGCTAATCTA+CGG + chr2_1:8321011-8321030 None:intergenic 35.0%
ATCTCGTAAAAACTGATGTG+TGG - chr2_1:8321070-8321089 Msa0192490:intron 35.0%
TAAAAACTGATGTGTGGCTA+TGG - chr2_1:8321076-8321095 Msa0192490:CDS 35.0%
AATGGATCTTATAAGGGAGA+AGG + chr2_1:8321222-8321241 None:intergenic 35.0%
CTCCCTTATAAGATCCATTA+TGG - chr2_1:8321223-8321242 Msa0192490:five_prime_UTR 35.0%
!!! ATTCCTATAGGGTTTTGGGA+AGG + chr2_1:8319356-8319375 None:intergenic 40.0%
AAAAAACCCACCATAACCTC+CGG - chr2_1:8319393-8319412 Msa0192490:three_prime_UTR 40.0%
! TTTGTTGTGTAGATTGCCAG+TGG - chr2_1:8319635-8319654 Msa0192490:three_prime_UTR 40.0%
CAACCAAACAAGCGCAAAAT+CGG + chr2_1:8319814-8319833 None:intergenic 40.0%
AGACTTTACATGGTGACTCT+AGG - chr2_1:8319957-8319976 Msa0192490:intron 40.0%
GACTTTACATGGTGACTCTA+GGG - chr2_1:8319958-8319977 Msa0192490:intron 40.0%
GCACTTGTGACACTATTTAG+GGG - chr2_1:8320022-8320041 Msa0192490:intron 40.0%
GTTTAAGAGCCCGTTTGAAT+TGG + chr2_1:8320221-8320240 None:intergenic 40.0%
CATATTTCCATAAGCTCTCG+AGG - chr2_1:8320282-8320301 Msa0192490:intron 40.0%
GGGAAAACACACAGACAATT+AGG + chr2_1:8320439-8320458 None:intergenic 40.0%
ATCTTCTACCAAATGAAGGG+GGG + chr2_1:8320459-8320478 None:intergenic 40.0%
AAAAGGCCTAAGCCAATAAG+AGG + chr2_1:8320662-8320681 None:intergenic 40.0%
AAACAAAGATGGAGCCAACA+AGG + chr2_1:8320707-8320726 None:intergenic 40.0%
TCTCTTGCTCTCACAATGAA+AGG - chr2_1:8320868-8320887 Msa0192490:CDS 40.0%
! GTGGCTATGGTGGAATTTAA+TGG - chr2_1:8321089-8321108 Msa0192490:CDS 40.0%
GATCTTATAAGGGAGAAGGA+AGG + chr2_1:8321218-8321237 None:intergenic 40.0%
!!! ATTCCTATAGGGTTTTGGGA+AGG + chr2_1:8319356-8319375 None:intergenic 40.0%
AAAAAACCCACCATAACCTC+CGG - chr2_1:8319393-8319412 Msa0192490:three_prime_UTR 40.0%
! TTTGTTGTGTAGATTGCCAG+TGG - chr2_1:8319635-8319654 Msa0192490:three_prime_UTR 40.0%
CAACCAAACAAGCGCAAAAT+CGG + chr2_1:8319814-8319833 None:intergenic 40.0%
AGACTTTACATGGTGACTCT+AGG - chr2_1:8319957-8319976 Msa0192490:intron 40.0%
GACTTTACATGGTGACTCTA+GGG - chr2_1:8319958-8319977 Msa0192490:intron 40.0%
GCACTTGTGACACTATTTAG+GGG - chr2_1:8320022-8320041 Msa0192490:intron 40.0%
GTTTAAGAGCCCGTTTGAAT+TGG + chr2_1:8320221-8320240 None:intergenic 40.0%
CATATTTCCATAAGCTCTCG+AGG - chr2_1:8320282-8320301 Msa0192490:intron 40.0%
GGGAAAACACACAGACAATT+AGG + chr2_1:8320439-8320458 None:intergenic 40.0%
ATCTTCTACCAAATGAAGGG+GGG + chr2_1:8320459-8320478 None:intergenic 40.0%
AAAAGGCCTAAGCCAATAAG+AGG + chr2_1:8320662-8320681 None:intergenic 40.0%
AAACAAAGATGGAGCCAACA+AGG + chr2_1:8320707-8320726 None:intergenic 40.0%
TCTCTTGCTCTCACAATGAA+AGG - chr2_1:8320868-8320887 Msa0192490:CDS 40.0%
! GTGGCTATGGTGGAATTTAA+TGG - chr2_1:8321089-8321108 Msa0192490:CDS 40.0%
GATCTTATAAGGGAGAAGGA+AGG + chr2_1:8321218-8321237 None:intergenic 40.0%
AACATTGAGCATGCAGGTCT+AGG + chr2_1:8319500-8319519 None:intergenic 45.0%
GACCTGCATGCTCAATGTTT+CGG - chr2_1:8319501-8319520 Msa0192490:three_prime_UTR 45.0%
ATCCGAAACATTGAGCATGC+AGG + chr2_1:8319506-8319525 None:intergenic 45.0%
GAAGCTGTTGCAGTGTGATA+TGG + chr2_1:8319690-8319709 None:intergenic 45.0%
TCAGTTTCTCGCCACTCTTA+CGG - chr2_1:8319732-8319751 Msa0192490:intron 45.0%
AAGCTATCCTCGAGAGCTTA+TGG + chr2_1:8320292-8320311 None:intergenic 45.0%
! TGTGTTTTCCCCCCTTCATT+TGG - chr2_1:8320448-8320467 Msa0192490:intron 45.0%
!!! TGTACCTTTTTTGGATGGGG+AGG - chr2_1:8320743-8320762 Msa0192490:intron 45.0%
!!! GTACCTTTTTTGGATGGGGA+GGG - chr2_1:8320744-8320763 Msa0192490:intron 45.0%
TTTCCCTCCCCATCCAAAAA+AGG + chr2_1:8320750-8320769 None:intergenic 45.0%
TCTTCTCTTGCCCTACAAAG+GGG + chr2_1:8320906-8320925 None:intergenic 45.0%
GTCTTCTCTTGCCCTACAAA+GGG + chr2_1:8320907-8320926 None:intergenic 45.0%
AGTCTTCTCTTGCCCTACAA+AGG + chr2_1:8320908-8320927 None:intergenic 45.0%
TGAAGAGCGAGCATCAAAGA+TGG - chr2_1:8320931-8320950 Msa0192490:CDS 45.0%
GCATCAAAGATGGTGTGACA+TGG - chr2_1:8320941-8320960 Msa0192490:CDS 45.0%
AAACTGATGTGTGGCTATGG+TGG - chr2_1:8321079-8321098 Msa0192490:CDS 45.0%
!!! TATAGGGTTTTGGGAAGGAG+GGG + chr2_1:8319351-8319370 None:intergenic 45.0%
!!! CTATAGGGTTTTGGGAAGGA+GGG + chr2_1:8319352-8319371 None:intergenic 45.0%
AACATTGAGCATGCAGGTCT+AGG + chr2_1:8319500-8319519 None:intergenic 45.0%
GACCTGCATGCTCAATGTTT+CGG - chr2_1:8319501-8319520 Msa0192490:three_prime_UTR 45.0%
ATCCGAAACATTGAGCATGC+AGG + chr2_1:8319506-8319525 None:intergenic 45.0%
GAAGCTGTTGCAGTGTGATA+TGG + chr2_1:8319690-8319709 None:intergenic 45.0%
TCAGTTTCTCGCCACTCTTA+CGG - chr2_1:8319732-8319751 Msa0192490:intron 45.0%
AAGCTATCCTCGAGAGCTTA+TGG + chr2_1:8320292-8320311 None:intergenic 45.0%
! TGTGTTTTCCCCCCTTCATT+TGG - chr2_1:8320448-8320467 Msa0192490:intron 45.0%
!!! TGTACCTTTTTTGGATGGGG+AGG - chr2_1:8320743-8320762 Msa0192490:intron 45.0%
!!! GTACCTTTTTTGGATGGGGA+GGG - chr2_1:8320744-8320763 Msa0192490:intron 45.0%
TTTCCCTCCCCATCCAAAAA+AGG + chr2_1:8320750-8320769 None:intergenic 45.0%
TCTTCTCTTGCCCTACAAAG+GGG + chr2_1:8320906-8320925 None:intergenic 45.0%
GTCTTCTCTTGCCCTACAAA+GGG + chr2_1:8320907-8320926 None:intergenic 45.0%
AGTCTTCTCTTGCCCTACAA+AGG + chr2_1:8320908-8320927 None:intergenic 45.0%
TGAAGAGCGAGCATCAAAGA+TGG - chr2_1:8320931-8320950 Msa0192490:CDS 45.0%
GCATCAAAGATGGTGTGACA+TGG - chr2_1:8320941-8320960 Msa0192490:CDS 45.0%
AAACTGATGTGTGGCTATGG+TGG - chr2_1:8321079-8321098 Msa0192490:CDS 45.0%
!!! CCTATAGGGTTTTGGGAAGG+AGG + chr2_1:8319353-8319372 None:intergenic 50.0%
CCTCCTTCCCAAAACCCTAT+AGG - chr2_1:8319350-8319369 Msa0192490:three_prime_UTR 50.0%
ACATCGTTAGGGACGAACAC+CGG + chr2_1:8319415-8319434 None:intergenic 50.0%
TGAGCATGCAGGTCTAGGTT+TGG + chr2_1:8319495-8319514 None:intergenic 50.0%
TTGCCAGTGGAGATGAGTTC+CGG - chr2_1:8319648-8319667 Msa0192490:three_prime_UTR 50.0%
TGGAAGCAAAGATTCGACGC+CGG + chr2_1:8319670-8319689 None:intergenic 50.0%
!!! TCGCCGATTTTGCGCTTGTT+TGG - chr2_1:8319808-8319827 Msa0192490:intron 50.0%
!! TGGTGACTCTAGGGCTTGTT+TGG - chr2_1:8319967-8319986 Msa0192490:intron 50.0%
GACACTATTTAGGGGCCGTT+TGG - chr2_1:8320030-8320049 Msa0192490:intron 50.0%
CAGACAATTAGGACCCCGTT+TGG + chr2_1:8320428-8320447 None:intergenic 50.0%
!!! CCTATAGGGTTTTGGGAAGG+AGG + chr2_1:8319353-8319372 None:intergenic 50.0%
CCTCCTTCCCAAAACCCTAT+AGG - chr2_1:8319350-8319369 Msa0192490:three_prime_UTR 50.0%
ACATCGTTAGGGACGAACAC+CGG + chr2_1:8319415-8319434 None:intergenic 50.0%
TGAGCATGCAGGTCTAGGTT+TGG + chr2_1:8319495-8319514 None:intergenic 50.0%
TTGCCAGTGGAGATGAGTTC+CGG - chr2_1:8319648-8319667 Msa0192490:three_prime_UTR 50.0%
TGGAAGCAAAGATTCGACGC+CGG + chr2_1:8319670-8319689 None:intergenic 50.0%
!!! TCGCCGATTTTGCGCTTGTT+TGG - chr2_1:8319808-8319827 Msa0192490:intron 50.0%
!! TGGTGACTCTAGGGCTTGTT+TGG - chr2_1:8319967-8319986 Msa0192490:intron 50.0%
GACACTATTTAGGGGCCGTT+TGG - chr2_1:8320030-8320049 Msa0192490:intron 50.0%
CAGACAATTAGGACCCCGTT+TGG + chr2_1:8320428-8320447 None:intergenic 50.0%
CGAACACCGGAGGTTATGGT+GGG + chr2_1:8319402-8319421 None:intergenic 55.0%
ACGAACACCGGAGGTTATGG+TGG + chr2_1:8319403-8319422 None:intergenic 55.0%
TCTGGCGGCTGACATCGTTA+GGG + chr2_1:8319426-8319445 None:intergenic 55.0%
ATCTGGCGGCTGACATCGTT+AGG + chr2_1:8319427-8319446 None:intergenic 55.0%
! TGCAGGTCTAGGTTTGGCTC+CGG + chr2_1:8319489-8319508 None:intergenic 55.0%
TTTCTCGCCACTCTTACGGC+TGG - chr2_1:8319736-8319755 Msa0192490:intron 55.0%
AAGGAAGGAGACAGAGGGAG+AGG + chr2_1:8321203-8321222 None:intergenic 55.0%
GGGAGAAGGAAGGAGACAGA+GGG + chr2_1:8321208-8321227 None:intergenic 55.0%
AGGGAGAAGGAAGGAGACAG+AGG + chr2_1:8321209-8321228 None:intergenic 55.0%
CGAACACCGGAGGTTATGGT+GGG + chr2_1:8319402-8319421 None:intergenic 55.0%
ACGAACACCGGAGGTTATGG+TGG + chr2_1:8319403-8319422 None:intergenic 55.0%
TCTGGCGGCTGACATCGTTA+GGG + chr2_1:8319426-8319445 None:intergenic 55.0%
ATCTGGCGGCTGACATCGTT+AGG + chr2_1:8319427-8319446 None:intergenic 55.0%
! TGCAGGTCTAGGTTTGGCTC+CGG + chr2_1:8319489-8319508 None:intergenic 55.0%
TTTCTCGCCACTCTTACGGC+TGG - chr2_1:8319736-8319755 Msa0192490:intron 55.0%
AAGGAAGGAGACAGAGGGAG+AGG + chr2_1:8321203-8321222 None:intergenic 55.0%
GGGAGAAGGAAGGAGACAGA+GGG + chr2_1:8321208-8321227 None:intergenic 55.0%
AGGGAGAAGGAAGGAGACAG+AGG + chr2_1:8321209-8321228 None:intergenic 55.0%
GGGACGAACACCGGAGGTTA+TGG + chr2_1:8319406-8319425 None:intergenic 60.0%
TCGTTAGGGACGAACACCGG+AGG + chr2_1:8319412-8319431 None:intergenic 60.0%
!! GGAGCGAAGGAAGCATCTGG+CGG + chr2_1:8319441-8319460 None:intergenic 60.0%
ACGCCGGAACTCATCTCCAC+TGG + chr2_1:8319654-8319673 None:intergenic 60.0%
CCACTCTTACGGCTGGACTC+CGG - chr2_1:8319743-8319762 Msa0192490:intron 60.0%
TCTTACGGCTGGACTCCGGA+AGG - chr2_1:8319747-8319766 Msa0192490:intron 60.0%
GGGACGAACACCGGAGGTTA+TGG + chr2_1:8319406-8319425 None:intergenic 60.0%
TCGTTAGGGACGAACACCGG+AGG + chr2_1:8319412-8319431 None:intergenic 60.0%
!! GGAGCGAAGGAAGCATCTGG+CGG + chr2_1:8319441-8319460 None:intergenic 60.0%
ACGCCGGAACTCATCTCCAC+TGG + chr2_1:8319654-8319673 None:intergenic 60.0%
CCACTCTTACGGCTGGACTC+CGG - chr2_1:8319743-8319762 Msa0192490:intron 60.0%
TCTTACGGCTGGACTCCGGA+AGG - chr2_1:8319747-8319766 Msa0192490:intron 60.0%
!! GGCGGAGCGAAGGAAGCATC+TGG + chr2_1:8319444-8319463 None:intergenic 65.0%
! AGCCAGGTTGGCGCGAGAAG+CGG + chr2_1:8319465-8319484 None:intergenic 65.0%
! TTTGGCTCCGGCAGCCAGGT+TGG + chr2_1:8319477-8319496 None:intergenic 65.0%
! TAGGTTTGGCTCCGGCAGCC+AGG + chr2_1:8319481-8319500 None:intergenic 65.0%
CCGGAGTCCAGCCGTAAGAG+TGG + chr2_1:8319746-8319765 None:intergenic 65.0%
!! GGCGGAGCGAAGGAAGCATC+TGG + chr2_1:8319444-8319463 None:intergenic 65.0%
! AGCCAGGTTGGCGCGAGAAG+CGG + chr2_1:8319465-8319484 None:intergenic 65.0%
! TTTGGCTCCGGCAGCCAGGT+TGG + chr2_1:8319477-8319496 None:intergenic 65.0%
! TAGGTTTGGCTCCGGCAGCC+AGG + chr2_1:8319481-8319500 None:intergenic 65.0%
CCGGAGTCCAGCCGTAAGAG+TGG + chr2_1:8319746-8319765 None:intergenic 65.0%
! CAGGTTGGCGCGAGAAGCGG+CGG + chr2_1:8319462-8319481 None:intergenic 70.0%
TCTCGCGCCAACCTGGCTGC+CGG - chr2_1:8319467-8319486 Msa0192490:three_prime_UTR 70.0%
! CAGGTTGGCGCGAGAAGCGG+CGG + chr2_1:8319462-8319481 None:intergenic 70.0%
TCTCGCGCCAACCTGGCTGC+CGG - chr2_1:8319467-8319486 Msa0192490:three_prime_UTR 70.0%
CGCGAGAAGCGGCGGAGCGA+AGG + chr2_1:8319454-8319473 None:intergenic 75.0%
CGCCGCTTCTCGCGCCAACC+TGG - chr2_1:8319460-8319479 Msa0192490:three_prime_UTR 75.0%
CGCGAGAAGCGGCGGAGCGA+AGG + chr2_1:8319454-8319473 None:intergenic 75.0%
CGCCGCTTCTCGCGCCAACC+TGG - chr2_1:8319460-8319479 Msa0192490:three_prime_UTR 75.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 8319350 8321282 8319350 ID=Msa0192490;Name=Msa0192490
chr2_1 mRNA 8319350 8321282 8319350 ID=Msa0192490-mRNA-1;Parent=Msa0192490;Name=Msa0192490-mRNA-1;_AED=0.27;_eAED=0.27;_QI=81|1|1|1|0.5|1|3|349|90
chr2_1 exon 8319350 8319718 8319350 ID=Msa0192490-mRNA-1:exon:3856;Parent=Msa0192490-mRNA-1
chr2_1 exon 8320864 8320985 8320864 ID=Msa0192490-mRNA-1:exon:3855;Parent=Msa0192490-mRNA-1
chr2_1 exon 8321071 8321282 8321071 ID=Msa0192490-mRNA-1:exon:3854;Parent=Msa0192490-mRNA-1
chr2_1 five_prime_UTR 8321202 8321282 8321202 ID=Msa0192490-mRNA-1:five_prime_utr;Parent=Msa0192490-mRNA-1
chr2_1 CDS 8321071 8321201 8321071 ID=Msa0192490-mRNA-1:cds;Parent=Msa0192490-mRNA-1
chr2_1 CDS 8320864 8320985 8320864 ID=Msa0192490-mRNA-1:cds;Parent=Msa0192490-mRNA-1
chr2_1 CDS 8319699 8319718 8319699 ID=Msa0192490-mRNA-1:cds;Parent=Msa0192490-mRNA-1
chr2_1 three_prime_UTR 8319350 8319698 8319350 ID=Msa0192490-mRNA-1:three_prime_utr;Parent=Msa0192490-mRNA-1
Gene Sequence

>Msa0192490

ATGTCAGCCGCCAGATGCTTCCTTCGCTCCGCCGCTTCTCGCGCCAACCTGGCTGCCGGAGCCAAACCTAGACCTGCATGCTCAATGTTTCGGATCCCAAAACAAACCTCAATTTCCAATCGCATTTCAAGATTGCCAGTGGAGATGAGTTCCGGCGTCGAATCTTTGCTTCCATATCACACTGCAACAGCTTCTGCATTGCTCACTTCAATGCTTTCAGTTTCTCGCCACTCTTACGGCTGGACTCCGGAAGGGCAAGAGAAGACTAGATGA
Protein sequence

>Msa0192490

MSAARCFLRSAASRANLAAGAKPRPACSMFRIPKQTSISNRISRLPVEMSSGVESLLPYHTATASALLTSMLSVSRHSYGWTPEGQEKTR*