AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0197980

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0197980 MtrunA17_Chr4g0052801 100.000 80 0 0 1 80 1 80 7.71e-56 166
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0197980 AT1G47278.3 57.500 80 34 0 1 80 1 80 4.68e-31 104
Msa0197980 AT1G47278.1 57.500 80 34 0 1 80 1 80 4.68e-31 104
Msa0197980 AT1G47278.2 57.500 80 34 0 1 80 12 91 6.52e-31 104

Find 46 sgRNAs with CRISPR-Local

Find 318 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTACATCGTAAATTGAATT+TGG 0.189960 2_1:-16400856 None:intergenic
GAGGAATCAGATAAATTAAT+TGG 0.258161 2_1:-16400909 None:intergenic
TTGAGACGGTGTAGATAGTT+TGG 0.292821 2_1:+16400832 Msa0197980:five_prime_UTR
ATAAATTAATTGGATGGGTT+TGG 0.310332 2_1:-16400899 None:intergenic
GATTTCAAGTTGTAGGATAA+TGG 0.313664 2_1:+16402488 Msa0197980:intron
TAAATTAATTGGATGGGTTT+GGG 0.328192 2_1:-16400898 None:intergenic
ATTAAAGATTCAATTGATAC+AGG 0.372142 2_1:-16403024 None:intergenic
ATTTCAAGTTGTAGGATAAT+GGG 0.372893 2_1:+16402489 Msa0197980:intron
AGGATAATGGGGCGGAAAGC+TGG 0.409342 2_1:+16402501 Msa0197980:exon
CGCCGGCTTACTCTCCGTTC+AGG 0.416223 2_1:+16400960 Msa0197980:five_prime_UTR
AATCAGATAAATTAATTGGA+TGG 0.433991 2_1:-16400905 None:intergenic
TTTCCTCGGTTCTCTCTTTG+CGG 0.442316 2_1:+16401022 Msa0197980:five_prime_UTR
TGAGACGGTGTAGATAGTTT+GGG 0.444138 2_1:+16400833 Msa0197980:five_prime_UTR
GGCGAGGATCGGTTGTGAAA+TGG 0.449428 2_1:-16400941 None:intergenic
ATCAGATAAATTAATTGGAT+GGG 0.462852 2_1:-16400904 None:intergenic
TTAAAATGTCGGCGTGGATG+AGG 0.477051 2_1:-16400995 None:intergenic
TTTAGTTAAGTTGAAAGAGT+TGG 0.483286 2_1:+16402967 Msa0197980:three_prime_UTR
AAATACATGTATAGAATCTC+AGG 0.487514 2_1:+16402656 Msa0197980:CDS
TAATGGGGCGGAAAGCTGGC+GGG 0.509687 2_1:+16402505 Msa0197980:exon
ACTTCATAAACCTTGCATGA+AGG 0.526107 2_1:+16402557 Msa0197980:CDS
CATCAAACTACTTCCTTCCA+CGG 0.534795 2_1:-16402872 None:intergenic
GATCATGTTTATGAACTGTA+TGG 0.548690 2_1:+16402584 Msa0197980:CDS
ATGATCATTTCCTTCATGCA+AGG 0.553694 2_1:-16402567 None:intergenic
ATTTCACAACCGATCCTCGC+CGG 0.556621 2_1:+16400943 Msa0197980:five_prime_UTR
ATAATGGGGCGGAAAGCTGG+CGG 0.563977 2_1:+16402504 Msa0197980:exon
GAGAGTAAGCCGGCGAGGAT+CGG 0.569692 2_1:-16400952 None:intergenic
TGCAGAGGCTTAGCCGTGGA+AGG 0.569997 2_1:+16402859 Msa0197980:CDS
GAGGAAATCGATTAAAATGT+CGG 0.596074 2_1:-16401006 None:intergenic
GAAACAATATGACAAGGTGA+TGG 0.605169 2_1:-16403069 None:intergenic
GGATCGGTTGTGAAATGGAA+CGG 0.620195 2_1:-16400936 None:intergenic
GGTTCAGACGGTGACCTGAA+CGG 0.629887 2_1:-16400974 None:intergenic
TGAAGCATGTGCTAAGCAGA+AGG 0.631587 2_1:+16402626 Msa0197980:CDS
GACCTGAACGGAGAGTAAGC+CGG 0.631903 2_1:-16400962 None:intergenic
TGATACAGGAAATATTACAT+AGG 0.631999 2_1:-16403010 None:intergenic
TGTGAAATGGAACGGAAGAG+AGG 0.636870 2_1:-16400928 None:intergenic
TTTCAAGTTGTAGGATAATG+GGG 0.640667 2_1:+16402490 Msa0197980:intron
CGGCGTGGATGAGGTTCAGA+CGG 0.645702 2_1:-16400986 None:intergenic
GTATCAATTATCAACTGCAG+AGG 0.657183 2_1:+16402844 Msa0197980:CDS
GAACGGAGAGTAAGCCGGCG+AGG 0.676490 2_1:-16400957 None:intergenic
CAAGTTGTAGGATAATGGGG+CGG 0.699621 2_1:+16402493 Msa0197980:intron
AGGGCAGAAACAATATGACA+AGG 0.700425 2_1:-16403075 None:intergenic
AAAAGAACAGAAAGTCTTGG+GGG 0.704646 2_1:+16402820 Msa0197980:CDS
CAACTGCAGAGGCTTAGCCG+TGG 0.708407 2_1:+16402855 Msa0197980:CDS
AATCGATTAAAATGTCGGCG+TGG 0.719973 2_1:-16401001 None:intergenic
CACATGCTTCAACATCACTG+TGG 0.742984 2_1:-16402614 None:intergenic
TTACCGCAAAGAGAGAACCG+AGG 0.757536 2_1:-16401025 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATCAAATCATATATTTAA+TGG + chr2_1:16402146-16402165 Msa0197980:intron 10.0%
!! AAATCAAATCATATATTTAA+TGG + chr2_1:16402146-16402165 Msa0197980:intron 10.0%
!!! TAATTAGTTCTCTATTTTAA+TGG + chr2_1:16401090-16401109 Msa0197980:intron 15.0%
!!! TTTTTTTTTTTTTTTACCTG+TGG + chr2_1:16401654-16401673 Msa0197980:intron 15.0%
!! ATTTACAAACATAATAACTT+TGG - chr2_1:16401903-16401922 None:intergenic 15.0%
!!! TTTTGAAATTTATATGATGA+AGG + chr2_1:16402077-16402096 Msa0197980:intron 15.0%
!!! TAATTAGTTCTCTATTTTAA+TGG + chr2_1:16401090-16401109 Msa0197980:intron 15.0%
!!! TTTTTTTTTTTTTTTACCTG+TGG + chr2_1:16401654-16401673 Msa0197980:intron 15.0%
!! ATTTACAAACATAATAACTT+TGG - chr2_1:16401903-16401922 None:intergenic 15.0%
!!! TTTTGAAATTTATATGATGA+AGG + chr2_1:16402077-16402096 Msa0197980:intron 15.0%
!! TTTACATCGTAAATTGAATT+TGG - chr2_1:16400859-16400878 None:intergenic 20.0%
!! ATCAGATAAATTAATTGGAT+GGG - chr2_1:16400907-16400926 None:intergenic 20.0%
!! AATCAGATAAATTAATTGGA+TGG - chr2_1:16400908-16400927 None:intergenic 20.0%
!! AATAGTTAGTGTATGAAATT+TGG + chr2_1:16401132-16401151 Msa0197980:intron 20.0%
!! ATATGTTTCTTATGAGAAAT+GGG + chr2_1:16401246-16401265 Msa0197980:intron 20.0%
!!! AGTATATGATGTGTTTTATT+GGG + chr2_1:16401713-16401732 Msa0197980:intron 20.0%
!! ATGAAGGAAATTTATTTGAA+GGG + chr2_1:16402093-16402112 Msa0197980:intron 20.0%
!! TTTGTTGATCAATAAATCAT+TGG + chr2_1:16402117-16402136 Msa0197980:intron 20.0%
!! TCAAATCATATATTTAATGG+TGG + chr2_1:16402149-16402168 Msa0197980:intron 20.0%
!! CAAATCATATATTTAATGGT+GGG + chr2_1:16402150-16402169 Msa0197980:intron 20.0%
!! CAAACTATAGTACTTTAAAT+AGG - chr2_1:16402251-16402270 None:intergenic 20.0%
!!! ATGAAAACTGTTTTTTGATT+TGG - chr2_1:16402457-16402476 None:intergenic 20.0%
!!! AAAAACAGTTTTCATTTTTC+TGG + chr2_1:16402462-16402481 Msa0197980:intron 20.0%
!!! TTTAGTTTGTTAAAATCTGA+CGG + chr2_1:16402689-16402708 Msa0197980:intron 20.0%
!! ATTAAAGATTCAATTGATAC+AGG - chr2_1:16403027-16403046 None:intergenic 20.0%
!! TTTACATCGTAAATTGAATT+TGG - chr2_1:16400859-16400878 None:intergenic 20.0%
!! ATCAGATAAATTAATTGGAT+GGG - chr2_1:16400907-16400926 None:intergenic 20.0%
!! AATCAGATAAATTAATTGGA+TGG - chr2_1:16400908-16400927 None:intergenic 20.0%
!! AATAGTTAGTGTATGAAATT+TGG + chr2_1:16401132-16401151 Msa0197980:intron 20.0%
!! ATATGTTTCTTATGAGAAAT+GGG + chr2_1:16401246-16401265 Msa0197980:intron 20.0%
!!! AGTATATGATGTGTTTTATT+GGG + chr2_1:16401713-16401732 Msa0197980:intron 20.0%
!! ATGAAGGAAATTTATTTGAA+GGG + chr2_1:16402093-16402112 Msa0197980:intron 20.0%
!! TTTGTTGATCAATAAATCAT+TGG + chr2_1:16402117-16402136 Msa0197980:intron 20.0%
!! TCAAATCATATATTTAATGG+TGG + chr2_1:16402149-16402168 Msa0197980:intron 20.0%
!! CAAATCATATATTTAATGGT+GGG + chr2_1:16402150-16402169 Msa0197980:intron 20.0%
!! CAAACTATAGTACTTTAAAT+AGG - chr2_1:16402251-16402270 None:intergenic 20.0%
!!! ATGAAAACTGTTTTTTGATT+TGG - chr2_1:16402457-16402476 None:intergenic 20.0%
!!! AAAAACAGTTTTCATTTTTC+TGG + chr2_1:16402462-16402481 Msa0197980:intron 20.0%
!!! TTTAGTTTGTTAAAATCTGA+CGG + chr2_1:16402689-16402708 Msa0197980:intron 20.0%
!! ATTAAAGATTCAATTGATAC+AGG - chr2_1:16403027-16403046 None:intergenic 20.0%
!! TAAATTAATTGGATGGGTTT+GGG - chr2_1:16400901-16400920 None:intergenic 25.0%
!! ATAAATTAATTGGATGGGTT+TGG - chr2_1:16400902-16400921 None:intergenic 25.0%
! GAGGAATCAGATAAATTAAT+TGG - chr2_1:16400912-16400931 None:intergenic 25.0%
! TATCTACAATTATGCATTGT+TGG + chr2_1:16401060-16401079 Msa0197980:intron 25.0%
! ATCTACAATTATGCATTGTT+GGG + chr2_1:16401061-16401080 Msa0197980:intron 25.0%
! TTCATACACTAACTATTGTA+GGG - chr2_1:16401129-16401148 None:intergenic 25.0%
! TTTCATACACTAACTATTGT+AGG - chr2_1:16401130-16401149 None:intergenic 25.0%
! TAGTGTATGAAATTTGGTTA+TGG + chr2_1:16401138-16401157 Msa0197980:intron 25.0%
! TTCATAAATTAACCTTTGCA+TGG + chr2_1:16401204-16401223 Msa0197980:intron 25.0%
! GATATGTTTCTTATGAGAAA+TGG + chr2_1:16401245-16401264 Msa0197980:intron 25.0%
! CTCCTTATCTTAAAAGAAAT+GGG - chr2_1:16401373-16401392 None:intergenic 25.0%
! TCTCCTTATCTTAAAAGAAA+TGG - chr2_1:16401374-16401393 None:intergenic 25.0%
!!! ATTTCTTTTAAGATAAGGAG+AGG + chr2_1:16401373-16401392 Msa0197980:intron 25.0%
!! CAGTATATGATGTGTTTTAT+TGG + chr2_1:16401712-16401731 Msa0197980:intron 25.0%
! CAAATTATAAAAATCTCACG+TGG - chr2_1:16402008-16402027 None:intergenic 25.0%
!! CACGTGAGATTTTTATAATT+TGG + chr2_1:16402006-16402025 Msa0197980:intron 25.0%
! GATGAAGGAAATTTATTTGA+AGG + chr2_1:16402092-16402111 Msa0197980:intron 25.0%
!!! ATTTCAAGTTGTAGGATAAT+GGG + chr2_1:16402489-16402508 Msa0197980:intron 25.0%
! AAATACATGTATAGAATCTC+AGG + chr2_1:16402656-16402675 Msa0197980:CDS 25.0%
! AAAAGAAAATCATGAGAAGA+GGG - chr2_1:16402728-16402747 None:intergenic 25.0%
! AAAAAGAAAATCATGAGAAG+AGG - chr2_1:16402729-16402748 None:intergenic 25.0%
! TAAAAAGAACAGAAAGTCTT+GGG + chr2_1:16402818-16402837 Msa0197980:CDS 25.0%
!!! TTTAGTTAAGTTGAAAGAGT+TGG + chr2_1:16402967-16402986 Msa0197980:three_prime_UTR 25.0%
! TGATACAGGAAATATTACAT+AGG - chr2_1:16403013-16403032 None:intergenic 25.0%
!! TAAATTAATTGGATGGGTTT+GGG - chr2_1:16400901-16400920 None:intergenic 25.0%
!! ATAAATTAATTGGATGGGTT+TGG - chr2_1:16400902-16400921 None:intergenic 25.0%
! GAGGAATCAGATAAATTAAT+TGG - chr2_1:16400912-16400931 None:intergenic 25.0%
! TATCTACAATTATGCATTGT+TGG + chr2_1:16401060-16401079 Msa0197980:intron 25.0%
! ATCTACAATTATGCATTGTT+GGG + chr2_1:16401061-16401080 Msa0197980:intron 25.0%
! TTCATACACTAACTATTGTA+GGG - chr2_1:16401129-16401148 None:intergenic 25.0%
! TTTCATACACTAACTATTGT+AGG - chr2_1:16401130-16401149 None:intergenic 25.0%
! TAGTGTATGAAATTTGGTTA+TGG + chr2_1:16401138-16401157 Msa0197980:intron 25.0%
! TTCATAAATTAACCTTTGCA+TGG + chr2_1:16401204-16401223 Msa0197980:intron 25.0%
! GATATGTTTCTTATGAGAAA+TGG + chr2_1:16401245-16401264 Msa0197980:intron 25.0%
! CTCCTTATCTTAAAAGAAAT+GGG - chr2_1:16401373-16401392 None:intergenic 25.0%
! TCTCCTTATCTTAAAAGAAA+TGG - chr2_1:16401374-16401393 None:intergenic 25.0%
!!! ATTTCTTTTAAGATAAGGAG+AGG + chr2_1:16401373-16401392 Msa0197980:intron 25.0%
!! CAGTATATGATGTGTTTTAT+TGG + chr2_1:16401712-16401731 Msa0197980:intron 25.0%
! CAAATTATAAAAATCTCACG+TGG - chr2_1:16402008-16402027 None:intergenic 25.0%
!! CACGTGAGATTTTTATAATT+TGG + chr2_1:16402006-16402025 Msa0197980:intron 25.0%
! GATGAAGGAAATTTATTTGA+AGG + chr2_1:16402092-16402111 Msa0197980:intron 25.0%
!!! ATTTCAAGTTGTAGGATAAT+GGG + chr2_1:16402489-16402508 Msa0197980:intron 25.0%
! AAATACATGTATAGAATCTC+AGG + chr2_1:16402656-16402675 Msa0197980:CDS 25.0%
! AAAAGAAAATCATGAGAAGA+GGG - chr2_1:16402728-16402747 None:intergenic 25.0%
! AAAAAGAAAATCATGAGAAG+AGG - chr2_1:16402729-16402748 None:intergenic 25.0%
! TAAAAAGAACAGAAAGTCTT+GGG + chr2_1:16402818-16402837 Msa0197980:CDS 25.0%
!!! TTTAGTTAAGTTGAAAGAGT+TGG + chr2_1:16402967-16402986 Msa0197980:three_prime_UTR 25.0%
! TGATACAGGAAATATTACAT+AGG - chr2_1:16403013-16403032 None:intergenic 25.0%
GAGGAAATCGATTAAAATGT+CGG - chr2_1:16401009-16401028 None:intergenic 30.0%
!! GACATTTTAATCGATTTCCT+CGG + chr2_1:16401008-16401027 Msa0197980:five_prime_UTR 30.0%
AATTTGGTTATGGTAGATGA+AGG + chr2_1:16401148-16401167 Msa0197980:intron 30.0%
ATTTGGTTATGGTAGATGAA+GGG + chr2_1:16401149-16401168 Msa0197980:intron 30.0%
!! CTACTCTAAAACCATAGTTA+CGG + chr2_1:16401342-16401361 Msa0197980:intron 30.0%
!!! TGCCCATTTCTTTTAAGATA+AGG + chr2_1:16401368-16401387 Msa0197980:intron 30.0%
TCAAATGTGTAACCTCATTA+GGG - chr2_1:16401692-16401711 None:intergenic 30.0%
!! ATGATGTGTTTTATTGGGTT+TGG + chr2_1:16401718-16401737 Msa0197980:intron 30.0%
ATCATATGCACACAGTATTT+CGG - chr2_1:16402033-16402052 None:intergenic 30.0%
TGAAATTACACTCTTCAGTT+GGG + chr2_1:16402280-16402299 Msa0197980:intron 30.0%
!!! GTTTTTTAATGTTGACTGCT+TGG + chr2_1:16402348-16402367 Msa0197980:intron 30.0%
!! ACAGTTTTCATTTTTCTGGT+TGG + chr2_1:16402466-16402485 Msa0197980:intron 30.0%
!! GATTTCAAGTTGTAGGATAA+TGG + chr2_1:16402488-16402507 Msa0197980:intron 30.0%
!! TTTCAAGTTGTAGGATAATG+GGG + chr2_1:16402490-16402509 Msa0197980:intron 30.0%
GATCATGTTTATGAACTGTA+TGG + chr2_1:16402584-16402603 Msa0197980:CDS 30.0%
GTAAAAAGAACAGAAAGTCT+TGG + chr2_1:16402817-16402836 Msa0197980:CDS 30.0%
AAAAAGAACAGAAAGTCTTG+GGG + chr2_1:16402819-16402838 Msa0197980:CDS 30.0%
GAGGAAATCGATTAAAATGT+CGG - chr2_1:16401009-16401028 None:intergenic 30.0%
!! GACATTTTAATCGATTTCCT+CGG + chr2_1:16401008-16401027 Msa0197980:five_prime_UTR 30.0%
AATTTGGTTATGGTAGATGA+AGG + chr2_1:16401148-16401167 Msa0197980:intron 30.0%
ATTTGGTTATGGTAGATGAA+GGG + chr2_1:16401149-16401168 Msa0197980:intron 30.0%
!! CTACTCTAAAACCATAGTTA+CGG + chr2_1:16401342-16401361 Msa0197980:intron 30.0%
!!! TGCCCATTTCTTTTAAGATA+AGG + chr2_1:16401368-16401387 Msa0197980:intron 30.0%
TCAAATGTGTAACCTCATTA+GGG - chr2_1:16401692-16401711 None:intergenic 30.0%
!! ATGATGTGTTTTATTGGGTT+TGG + chr2_1:16401718-16401737 Msa0197980:intron 30.0%
ATCATATGCACACAGTATTT+CGG - chr2_1:16402033-16402052 None:intergenic 30.0%
TGAAATTACACTCTTCAGTT+GGG + chr2_1:16402280-16402299 Msa0197980:intron 30.0%
!!! GTTTTTTAATGTTGACTGCT+TGG + chr2_1:16402348-16402367 Msa0197980:intron 30.0%
!! ACAGTTTTCATTTTTCTGGT+TGG + chr2_1:16402466-16402485 Msa0197980:intron 30.0%
!! GATTTCAAGTTGTAGGATAA+TGG + chr2_1:16402488-16402507 Msa0197980:intron 30.0%
!! TTTCAAGTTGTAGGATAATG+GGG + chr2_1:16402490-16402509 Msa0197980:intron 30.0%
GATCATGTTTATGAACTGTA+TGG + chr2_1:16402584-16402603 Msa0197980:CDS 30.0%
GTAAAAAGAACAGAAAGTCT+TGG + chr2_1:16402817-16402836 Msa0197980:CDS 30.0%
AAAAAGAACAGAAAGTCTTG+GGG + chr2_1:16402819-16402838 Msa0197980:CDS 30.0%
! TGGGTCATTTCGAAGTTTTA+GGG - chr2_1:16400882-16400901 None:intergenic 35.0%
! TTGGGTCATTTCGAAGTTTT+AGG - chr2_1:16400883-16400902 None:intergenic 35.0%
ATAAGATACTCGGTCGTTAT+AGG + chr2_1:16401410-16401429 Msa0197980:intron 35.0%
TACATGCCAATATGGATTCA+CGG - chr2_1:16401479-16401498 None:intergenic 35.0%
GTCAAATGTGTAACCTCATT+AGG - chr2_1:16401693-16401712 None:intergenic 35.0%
TGTGTACATGTATCACTGTT+TGG + chr2_1:16401922-16401941 Msa0197980:intron 35.0%
! GTATCACTGTTTGGTGAATT+TGG + chr2_1:16401931-16401950 Msa0197980:intron 35.0%
ATACCTATGTATAGAAGGAC+AGG - chr2_1:16401983-16402002 None:intergenic 35.0%
TGGCAATACCTATGTATAGA+AGG - chr2_1:16401988-16402007 None:intergenic 35.0%
TGTGCATATGATTTCCAACT+TGG + chr2_1:16402041-16402060 Msa0197980:intron 35.0%
GGTGGGATTTATTGATCTAA+TGG + chr2_1:16402167-16402186 Msa0197980:intron 35.0%
GGGATTTATTGATCTAATGG+TGG + chr2_1:16402170-16402189 Msa0197980:intron 35.0%
CTTTAAATAGGCCAACTCTA+TGG - chr2_1:16402239-16402258 None:intergenic 35.0%
! CAACTGAAGAGTGTAATTTC+AGG - chr2_1:16402281-16402300 None:intergenic 35.0%
CTGAAATTACACTCTTCAGT+TGG + chr2_1:16402279-16402298 Msa0197980:intron 35.0%
!!! TTTTTTGATTCCATGTGCAC+TGG + chr2_1:16402318-16402337 Msa0197980:intron 35.0%
ACTTCATAAACCTTGCATGA+AGG + chr2_1:16402557-16402576 Msa0197980:CDS 35.0%
ATGATCATTTCCTTCATGCA+AGG - chr2_1:16402570-16402589 None:intergenic 35.0%
AAAAGAACAGAAAGTCTTGG+GGG + chr2_1:16402820-16402839 Msa0197980:CDS 35.0%
GTATCAATTATCAACTGCAG+AGG + chr2_1:16402844-16402863 Msa0197980:CDS 35.0%
! TGGGTCATTTCGAAGTTTTA+GGG - chr2_1:16400882-16400901 None:intergenic 35.0%
! TTGGGTCATTTCGAAGTTTT+AGG - chr2_1:16400883-16400902 None:intergenic 35.0%
ATAAGATACTCGGTCGTTAT+AGG + chr2_1:16401410-16401429 Msa0197980:intron 35.0%
TACATGCCAATATGGATTCA+CGG - chr2_1:16401479-16401498 None:intergenic 35.0%
GTCAAATGTGTAACCTCATT+AGG - chr2_1:16401693-16401712 None:intergenic 35.0%
TGTGTACATGTATCACTGTT+TGG + chr2_1:16401922-16401941 Msa0197980:intron 35.0%
! GTATCACTGTTTGGTGAATT+TGG + chr2_1:16401931-16401950 Msa0197980:intron 35.0%
ATACCTATGTATAGAAGGAC+AGG - chr2_1:16401983-16402002 None:intergenic 35.0%
TGGCAATACCTATGTATAGA+AGG - chr2_1:16401988-16402007 None:intergenic 35.0%
TGTGCATATGATTTCCAACT+TGG + chr2_1:16402041-16402060 Msa0197980:intron 35.0%
GGTGGGATTTATTGATCTAA+TGG + chr2_1:16402167-16402186 Msa0197980:intron 35.0%
GGGATTTATTGATCTAATGG+TGG + chr2_1:16402170-16402189 Msa0197980:intron 35.0%
CTTTAAATAGGCCAACTCTA+TGG - chr2_1:16402239-16402258 None:intergenic 35.0%
! CAACTGAAGAGTGTAATTTC+AGG - chr2_1:16402281-16402300 None:intergenic 35.0%
CTGAAATTACACTCTTCAGT+TGG + chr2_1:16402279-16402298 Msa0197980:intron 35.0%
!!! TTTTTTGATTCCATGTGCAC+TGG + chr2_1:16402318-16402337 Msa0197980:intron 35.0%
ACTTCATAAACCTTGCATGA+AGG + chr2_1:16402557-16402576 Msa0197980:CDS 35.0%
ATGATCATTTCCTTCATGCA+AGG - chr2_1:16402570-16402589 None:intergenic 35.0%
AAAAGAACAGAAAGTCTTGG+GGG + chr2_1:16402820-16402839 Msa0197980:CDS 35.0%
GTATCAATTATCAACTGCAG+AGG + chr2_1:16402844-16402863 Msa0197980:CDS 35.0%
TTGAGACGGTGTAGATAGTT+TGG + chr2_1:16400832-16400851 Msa0197980:five_prime_UTR 40.0%
TGAGACGGTGTAGATAGTTT+GGG + chr2_1:16400833-16400852 Msa0197980:five_prime_UTR 40.0%
AATCGATTAAAATGTCGGCG+TGG - chr2_1:16401004-16401023 None:intergenic 40.0%
AACATATCCTTTACTCCCGA+GGG - chr2_1:16401233-16401252 None:intergenic 40.0%
AAACATATCCTTTACTCCCG+AGG - chr2_1:16401234-16401253 None:intergenic 40.0%
CTACCCGGTTATAAGATACT+CGG + chr2_1:16401400-16401419 Msa0197980:intron 40.0%
ACGACCGAGTATCTTATAAC+CGG - chr2_1:16401407-16401426 None:intergenic 40.0%
!! AGTTGGAAAGGTATTGCCAT+GGG + chr2_1:16401433-16401452 Msa0197980:intron 40.0%
ACATGCCAATATGGATTCAC+GGG - chr2_1:16401478-16401497 None:intergenic 40.0%
CCATAGCATACATGCCAATA+TGG - chr2_1:16401487-16401506 None:intergenic 40.0%
CCATATTGGCATGTATGCTA+TGG + chr2_1:16401484-16401503 Msa0197980:intron 40.0%
TTGGCATGTATGCTATGGAT+CGG + chr2_1:16401489-16401508 Msa0197980:intron 40.0%
ATGTGTAACCTCATTAGGGA+GGG - chr2_1:16401688-16401707 None:intergenic 40.0%
AATGTGTAACCTCATTAGGG+AGG - chr2_1:16401689-16401708 None:intergenic 40.0%
TTGGTGAATTTGGACCAACT+TGG + chr2_1:16401941-16401960 Msa0197980:intron 40.0%
CATACAAGACAAGACCAAGT+TGG - chr2_1:16401958-16401977 None:intergenic 40.0%
!! CAACTTGGTCTTGTCTTGTA+TGG + chr2_1:16401956-16401975 Msa0197980:intron 40.0%
AAGATATGCATACGCCAAGT+TGG - chr2_1:16402058-16402077 None:intergenic 40.0%
TCACTTGTGTACCATAGAGT+TGG + chr2_1:16402225-16402244 Msa0197980:intron 40.0%
!! CTGGTTGGATTTCAAGTTGT+AGG + chr2_1:16402481-16402500 Msa0197980:intron 40.0%
GAAAATCATGAGAAGAGGGA+GGG - chr2_1:16402724-16402743 None:intergenic 40.0%
AGAAAATCATGAGAAGAGGG+AGG - chr2_1:16402725-16402744 None:intergenic 40.0%
CATCAAACTACTTCCTTCCA+CGG - chr2_1:16402875-16402894 None:intergenic 40.0%
TTGAGACGGTGTAGATAGTT+TGG + chr2_1:16400832-16400851 Msa0197980:five_prime_UTR 40.0%
TGAGACGGTGTAGATAGTTT+GGG + chr2_1:16400833-16400852 Msa0197980:five_prime_UTR 40.0%
AATCGATTAAAATGTCGGCG+TGG - chr2_1:16401004-16401023 None:intergenic 40.0%
AACATATCCTTTACTCCCGA+GGG - chr2_1:16401233-16401252 None:intergenic 40.0%
AAACATATCCTTTACTCCCG+AGG - chr2_1:16401234-16401253 None:intergenic 40.0%
CTACCCGGTTATAAGATACT+CGG + chr2_1:16401400-16401419 Msa0197980:intron 40.0%
ACGACCGAGTATCTTATAAC+CGG - chr2_1:16401407-16401426 None:intergenic 40.0%
!! AGTTGGAAAGGTATTGCCAT+GGG + chr2_1:16401433-16401452 Msa0197980:intron 40.0%
ACATGCCAATATGGATTCAC+GGG - chr2_1:16401478-16401497 None:intergenic 40.0%
CCATAGCATACATGCCAATA+TGG - chr2_1:16401487-16401506 None:intergenic 40.0%
CCATATTGGCATGTATGCTA+TGG + chr2_1:16401484-16401503 Msa0197980:intron 40.0%
TTGGCATGTATGCTATGGAT+CGG + chr2_1:16401489-16401508 Msa0197980:intron 40.0%
ATGTGTAACCTCATTAGGGA+GGG - chr2_1:16401688-16401707 None:intergenic 40.0%
AATGTGTAACCTCATTAGGG+AGG - chr2_1:16401689-16401708 None:intergenic 40.0%
TTGGTGAATTTGGACCAACT+TGG + chr2_1:16401941-16401960 Msa0197980:intron 40.0%
CATACAAGACAAGACCAAGT+TGG - chr2_1:16401958-16401977 None:intergenic 40.0%
!! CAACTTGGTCTTGTCTTGTA+TGG + chr2_1:16401956-16401975 Msa0197980:intron 40.0%
AAGATATGCATACGCCAAGT+TGG - chr2_1:16402058-16402077 None:intergenic 40.0%
TCACTTGTGTACCATAGAGT+TGG + chr2_1:16402225-16402244 Msa0197980:intron 40.0%
!! CTGGTTGGATTTCAAGTTGT+AGG + chr2_1:16402481-16402500 Msa0197980:intron 40.0%
GAAAATCATGAGAAGAGGGA+GGG - chr2_1:16402724-16402743 None:intergenic 40.0%
AGAAAATCATGAGAAGAGGG+AGG - chr2_1:16402725-16402744 None:intergenic 40.0%
CATCAAACTACTTCCTTCCA+CGG - chr2_1:16402875-16402894 None:intergenic 40.0%
TGTGAAATGGAACGGAAGAG+AGG - chr2_1:16400931-16400950 None:intergenic 45.0%
GGATCGGTTGTGAAATGGAA+CGG - chr2_1:16400939-16400958 None:intergenic 45.0%
TTAAAATGTCGGCGTGGATG+AGG - chr2_1:16400998-16401017 None:intergenic 45.0%
TTTCCTCGGTTCTCTCTTTG+CGG + chr2_1:16401022-16401041 Msa0197980:five_prime_UTR 45.0%
AACCTTTGCATGGTAACCCT+CGG + chr2_1:16401214-16401233 Msa0197980:intron 45.0%
AATGGGCATCTCCGTAACTA+TGG - chr2_1:16401356-16401375 None:intergenic 45.0%
ATAAGGAGAGGATCTCTACC+CGG + chr2_1:16401385-16401404 Msa0197980:intron 45.0%
CGACCGAGTATCTTATAACC+GGG - chr2_1:16401406-16401425 None:intergenic 45.0%
TACTCGGTCGTTATAGGAGT+TGG + chr2_1:16401416-16401435 Msa0197980:intron 45.0%
GGTCGTTATAGGAGTTGGAA+AGG + chr2_1:16401421-16401440 Msa0197980:intron 45.0%
!! GAGTTGGAAAGGTATTGCCA+TGG + chr2_1:16401432-16401451 Msa0197980:intron 45.0%
ATATGGATTCACGGGACACA+CGG - chr2_1:16401470-16401489 None:intergenic 45.0%
TGTGTCCCGTGAATCCATAT+TGG + chr2_1:16401470-16401489 Msa0197980:intron 45.0%
TGGCATGTATGCTATGGATC+GGG + chr2_1:16401490-16401509 Msa0197980:intron 45.0%
!! TGACAAAAGAGTGTCCAACG+AGG + chr2_1:16401544-16401563 Msa0197980:intron 45.0%
TTGTAGCTGCACAGACTACT+GGG - chr2_1:16401629-16401648 None:intergenic 45.0%
GGACCTGTCCTTCTATACAT+AGG + chr2_1:16401977-16401996 Msa0197980:intron 45.0%
AACACTAATGCCAGTGCACA+TGG - chr2_1:16402331-16402350 None:intergenic 45.0%
!! CAAGTTGTAGGATAATGGGG+CGG + chr2_1:16402493-16402512 Msa0197980:intron 45.0%
CACATGCTTCAACATCACTG+TGG - chr2_1:16402617-16402636 None:intergenic 45.0%
TGAAGCATGTGCTAAGCAGA+AGG + chr2_1:16402626-16402645 Msa0197980:CDS 45.0%
TGTGAAATGGAACGGAAGAG+AGG - chr2_1:16400931-16400950 None:intergenic 45.0%
GGATCGGTTGTGAAATGGAA+CGG - chr2_1:16400939-16400958 None:intergenic 45.0%
TTAAAATGTCGGCGTGGATG+AGG - chr2_1:16400998-16401017 None:intergenic 45.0%
TTTCCTCGGTTCTCTCTTTG+CGG + chr2_1:16401022-16401041 Msa0197980:five_prime_UTR 45.0%
AACCTTTGCATGGTAACCCT+CGG + chr2_1:16401214-16401233 Msa0197980:intron 45.0%
AATGGGCATCTCCGTAACTA+TGG - chr2_1:16401356-16401375 None:intergenic 45.0%
ATAAGGAGAGGATCTCTACC+CGG + chr2_1:16401385-16401404 Msa0197980:intron 45.0%
CGACCGAGTATCTTATAACC+GGG - chr2_1:16401406-16401425 None:intergenic 45.0%
TACTCGGTCGTTATAGGAGT+TGG + chr2_1:16401416-16401435 Msa0197980:intron 45.0%
GGTCGTTATAGGAGTTGGAA+AGG + chr2_1:16401421-16401440 Msa0197980:intron 45.0%
!! GAGTTGGAAAGGTATTGCCA+TGG + chr2_1:16401432-16401451 Msa0197980:intron 45.0%
ATATGGATTCACGGGACACA+CGG - chr2_1:16401470-16401489 None:intergenic 45.0%
TGTGTCCCGTGAATCCATAT+TGG + chr2_1:16401470-16401489 Msa0197980:intron 45.0%
TGGCATGTATGCTATGGATC+GGG + chr2_1:16401490-16401509 Msa0197980:intron 45.0%
!! TGACAAAAGAGTGTCCAACG+AGG + chr2_1:16401544-16401563 Msa0197980:intron 45.0%
TTGTAGCTGCACAGACTACT+GGG - chr2_1:16401629-16401648 None:intergenic 45.0%
GGACCTGTCCTTCTATACAT+AGG + chr2_1:16401977-16401996 Msa0197980:intron 45.0%
AACACTAATGCCAGTGCACA+TGG - chr2_1:16402331-16402350 None:intergenic 45.0%
!! CAAGTTGTAGGATAATGGGG+CGG + chr2_1:16402493-16402512 Msa0197980:intron 45.0%
CACATGCTTCAACATCACTG+TGG - chr2_1:16402617-16402636 None:intergenic 45.0%
TGAAGCATGTGCTAAGCAGA+AGG + chr2_1:16402626-16402645 Msa0197980:CDS 45.0%
ATTTCACAACCGATCCTCGC+CGG + chr2_1:16400943-16400962 Msa0197980:five_prime_UTR 50.0%
TTACCGCAAAGAGAGAACCG+AGG - chr2_1:16401028-16401047 None:intergenic 50.0%
ACCTTTGCATGGTAACCCTC+GGG + chr2_1:16401215-16401234 Msa0197980:intron 50.0%
ATGGTAACCCTCGGGAGTAA+AGG + chr2_1:16401223-16401242 Msa0197980:intron 50.0%
! CACGGAGTTTTCTACACCCA+TGG - chr2_1:16401452-16401471 None:intergenic 50.0%
CTTGTAGCTGCACAGACTAC+TGG - chr2_1:16401630-16401649 None:intergenic 50.0%
ATTTCACAACCGATCCTCGC+CGG + chr2_1:16400943-16400962 Msa0197980:five_prime_UTR 50.0%
TTACCGCAAAGAGAGAACCG+AGG - chr2_1:16401028-16401047 None:intergenic 50.0%
ACCTTTGCATGGTAACCCTC+GGG + chr2_1:16401215-16401234 Msa0197980:intron 50.0%
ATGGTAACCCTCGGGAGTAA+AGG + chr2_1:16401223-16401242 Msa0197980:intron 50.0%
! CACGGAGTTTTCTACACCCA+TGG - chr2_1:16401452-16401471 None:intergenic 50.0%
CTTGTAGCTGCACAGACTAC+TGG - chr2_1:16401630-16401649 None:intergenic 50.0%
GGCGAGGATCGGTTGTGAAA+TGG - chr2_1:16400944-16400963 None:intergenic 55.0%
GACCTGAACGGAGAGTAAGC+CGG - chr2_1:16400965-16400984 None:intergenic 55.0%
GGTTCAGACGGTGACCTGAA+CGG - chr2_1:16400977-16400996 None:intergenic 55.0%
TCCCGAGGGTTACCATGCAA+AGG - chr2_1:16401219-16401238 None:intergenic 55.0%
TGTCCAACGAGGCAACGTCT+TGG + chr2_1:16401555-16401574 Msa0197980:intron 55.0%
GTCCAACGAGGCAACGTCTT+GGG + chr2_1:16401556-16401575 Msa0197980:intron 55.0%
AGGCAACGTCTTGGGTCGAT+GGG + chr2_1:16401564-16401583 Msa0197980:intron 55.0%
! ACCGAGTCTGGGTTGACATG+CGG + chr2_1:16401594-16401613 Msa0197980:intron 55.0%
CTCAGACTCCCTCCCTAATG+AGG + chr2_1:16401677-16401696 Msa0197980:intron 55.0%
!! AGGATAATGGGGCGGAAAGC+TGG + chr2_1:16402501-16402520 Msa0197980:exon 55.0%
! ATAATGGGGCGGAAAGCTGG+CGG + chr2_1:16402504-16402523 Msa0197980:exon 55.0%
GGCGAGGATCGGTTGTGAAA+TGG - chr2_1:16400944-16400963 None:intergenic 55.0%
GACCTGAACGGAGAGTAAGC+CGG - chr2_1:16400965-16400984 None:intergenic 55.0%
GGTTCAGACGGTGACCTGAA+CGG - chr2_1:16400977-16400996 None:intergenic 55.0%
TCCCGAGGGTTACCATGCAA+AGG - chr2_1:16401219-16401238 None:intergenic 55.0%
TGTCCAACGAGGCAACGTCT+TGG + chr2_1:16401555-16401574 Msa0197980:intron 55.0%
GTCCAACGAGGCAACGTCTT+GGG + chr2_1:16401556-16401575 Msa0197980:intron 55.0%
AGGCAACGTCTTGGGTCGAT+GGG + chr2_1:16401564-16401583 Msa0197980:intron 55.0%
! ACCGAGTCTGGGTTGACATG+CGG + chr2_1:16401594-16401613 Msa0197980:intron 55.0%
CTCAGACTCCCTCCCTAATG+AGG + chr2_1:16401677-16401696 Msa0197980:intron 55.0%
!! AGGATAATGGGGCGGAAAGC+TGG + chr2_1:16402501-16402520 Msa0197980:exon 55.0%
! ATAATGGGGCGGAAAGCTGG+CGG + chr2_1:16402504-16402523 Msa0197980:exon 55.0%
GAGAGTAAGCCGGCGAGGAT+CGG - chr2_1:16400955-16400974 None:intergenic 60.0%
CGGCGTGGATGAGGTTCAGA+CGG - chr2_1:16400989-16401008 None:intergenic 60.0%
GACCCAAGACGTTGCCTCGT+TGG - chr2_1:16401561-16401580 None:intergenic 60.0%
GAGGCAACGTCTTGGGTCGA+TGG + chr2_1:16401563-16401582 Msa0197980:intron 60.0%
CAACGTCTTGGGTCGATGGG+AGG + chr2_1:16401567-16401586 Msa0197980:intron 60.0%
GTCTTGGGTCGATGGGAGGA+GGG + chr2_1:16401571-16401590 Msa0197980:intron 60.0%
CCCGCATGTCAACCCAGACT+CGG - chr2_1:16401598-16401617 None:intergenic 60.0%
! CCGAGTCTGGGTTGACATGC+GGG + chr2_1:16401595-16401614 Msa0197980:intron 60.0%
TAATGGGGCGGAAAGCTGGC+GGG + chr2_1:16402505-16402524 Msa0197980:exon 60.0%
CAACTGCAGAGGCTTAGCCG+TGG + chr2_1:16402855-16402874 Msa0197980:CDS 60.0%
TGCAGAGGCTTAGCCGTGGA+AGG + chr2_1:16402859-16402878 Msa0197980:CDS 60.0%
GAGAGTAAGCCGGCGAGGAT+CGG - chr2_1:16400955-16400974 None:intergenic 60.0%
CGGCGTGGATGAGGTTCAGA+CGG - chr2_1:16400989-16401008 None:intergenic 60.0%
GACCCAAGACGTTGCCTCGT+TGG - chr2_1:16401561-16401580 None:intergenic 60.0%
GAGGCAACGTCTTGGGTCGA+TGG + chr2_1:16401563-16401582 Msa0197980:intron 60.0%
CAACGTCTTGGGTCGATGGG+AGG + chr2_1:16401567-16401586 Msa0197980:intron 60.0%
GTCTTGGGTCGATGGGAGGA+GGG + chr2_1:16401571-16401590 Msa0197980:intron 60.0%
CCCGCATGTCAACCCAGACT+CGG - chr2_1:16401598-16401617 None:intergenic 60.0%
! CCGAGTCTGGGTTGACATGC+GGG + chr2_1:16401595-16401614 Msa0197980:intron 60.0%
TAATGGGGCGGAAAGCTGGC+GGG + chr2_1:16402505-16402524 Msa0197980:exon 60.0%
CAACTGCAGAGGCTTAGCCG+TGG + chr2_1:16402855-16402874 Msa0197980:CDS 60.0%
TGCAGAGGCTTAGCCGTGGA+AGG + chr2_1:16402859-16402878 Msa0197980:CDS 60.0%
GAACGGAGAGTAAGCCGGCG+AGG - chr2_1:16400960-16400979 None:intergenic 65.0%
!! CGCCGGCTTACTCTCCGTTC+AGG + chr2_1:16400960-16400979 Msa0197980:five_prime_UTR 65.0%
CGTCTTGGGTCGATGGGAGG+AGG + chr2_1:16401570-16401589 Msa0197980:intron 65.0%
ATGGGAGGAGGGACCGAGTC+TGG + chr2_1:16401582-16401601 Msa0197980:intron 65.0%
TGGGAGGAGGGACCGAGTCT+GGG + chr2_1:16401583-16401602 Msa0197980:intron 65.0%
AGGGAGGGAGTCTGAGCCAC+AGG - chr2_1:16401673-16401692 None:intergenic 65.0%
GAACGGAGAGTAAGCCGGCG+AGG - chr2_1:16400960-16400979 None:intergenic 65.0%
!! CGCCGGCTTACTCTCCGTTC+AGG + chr2_1:16400960-16400979 Msa0197980:five_prime_UTR 65.0%
CGTCTTGGGTCGATGGGAGG+AGG + chr2_1:16401570-16401589 Msa0197980:intron 65.0%
ATGGGAGGAGGGACCGAGTC+TGG + chr2_1:16401582-16401601 Msa0197980:intron 65.0%
TGGGAGGAGGGACCGAGTCT+GGG + chr2_1:16401583-16401602 Msa0197980:intron 65.0%
AGGGAGGGAGTCTGAGCCAC+AGG - chr2_1:16401673-16401692 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 16400821 16403090 16400821 ID=Msa0197980;Name=Msa0197980
chr2_1 mRNA 16400821 16403090 16400821 ID=Msa0197980-mRNA-1;Parent=Msa0197980;Name=Msa0197980-mRNA-1;_AED=0.13;_eAED=0.13;_QI=233|1|1|1|0.5|0.33|3|203|80
chr2_1 exon 16400821 16401043 16400821 ID=Msa0197980-mRNA-1:exon:6785;Parent=Msa0197980-mRNA-1
chr2_1 exon 16402497 16402677 16402497 ID=Msa0197980-mRNA-1:exon:6786;Parent=Msa0197980-mRNA-1
chr2_1 exon 16402816 16403090 16402816 ID=Msa0197980-mRNA-1:exon:6787;Parent=Msa0197980-mRNA-1
chr2_1 five_prime_UTR 16400821 16401043 16400821 ID=Msa0197980-mRNA-1:five_prime_utr;Parent=Msa0197980-mRNA-1
chr2_1 five_prime_UTR 16402497 16402506 16402497 ID=Msa0197980-mRNA-1:five_prime_utr;Parent=Msa0197980-mRNA-1
chr2_1 CDS 16402507 16402677 16402507 ID=Msa0197980-mRNA-1:cds;Parent=Msa0197980-mRNA-1
chr2_1 CDS 16402816 16402887 16402816 ID=Msa0197980-mRNA-1:cds;Parent=Msa0197980-mRNA-1
chr2_1 three_prime_UTR 16402888 16403090 16402888 ID=Msa0197980-mRNA-1:three_prime_utr;Parent=Msa0197980-mRNA-1
Gene Sequence

>Msa0197980

ATGGGGCGGAAAGCTGGCGGGCTTTTTATCAACTCTAAGAGATTCAGTTCACTTCATAAACCTTGCATGAAGGAAATGATCATGTTTATGAACTGTATGGCTACAAGCCACAGTGATGTTGAAGCATGTGCTAAGCAGAAGGAAATATTAAATACATGTATAGAATCTCAGAGTAAAAAGAACAGAAAGTCTTGGGGGAGTATCAATTATCAACTGCAGAGGCTTAGCCGTGGAAGGAAGTAG
Protein sequence

>Msa0197980

MGRKAGGLFINSKRFSSLHKPCMKEMIMFMNCMATSHSDVEACAKQKEILNTCIESQSKKNRKSWGSINYQLQRLSRGRK*