AlfalfaGEDB | Msa0220640

M. sativa cultivar zhongmu-4 / Msa0220640

KEGG: K10666
GO: GO:0001101 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006464 GO:0006508 GO:0006511 GO:0006515 GO:0006807 GO:0006810 GO:0006886 GO:0006950 GO:0006970 GO:0008104 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009611 GO:0009628 GO:0009719 GO:0009723 GO:0009725 GO:0009987 GO:0010033 GO:0010035 GO:0010243 GO:0010498 GO:0012505 GO:0015031 GO:0015833 GO:0016020 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019899 GO:0019941 GO:0030163 GO:0031984 GO:0032446 GO:0032527 GO:0032991 GO:0033036 GO:0033554 GO:0034613 GO:0034976 GO:0035966 GO:0035967 GO:0036211 GO:0036503 GO:0036513 GO:0042175 GO:0042221 GO:0042886 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044390 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0046907 GO:0050896 GO:0051179 GO:0051234 GO:0051603 GO:0051641 GO:0051649 GO:0051716 GO:0051788 GO:0070647 GO:0070727 GO:0070887 GO:0071218 GO:0071310 GO:0071702 GO:0071704 GO:0071705 GO:0071712 GO:0098796 GO:0098827 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1901698 GO:1901700
PFAM: PF00097.28

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0220640	XP_024631933.1	93.567	171	3	2	1	171	1	163	2.56e-111	323

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0220640	sp\|Q6R567\|RMA1_CAPAN	42.236	161	82	5	5	154	10	170	7.84e-34	122

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0220640	A0A072VAR6	93.567	171	3	2	1	171	1	163	1.22e-111	323

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0005190	Msa0220640	0.800735	1.253589e-48	-8.615850e-47
Msa0072390	Msa0220640	0.810331	1.215361e-50	-8.615850e-47
Msa0157030	Msa0220640	0.801328	9.481598e-49	-8.615850e-47
Msa0163960	Msa0220640	0.830222	3.316641e-55	-8.615850e-47
Msa0212730	Msa0220640	0.824567	7.527836e-54	-8.615850e-47
Msa0220640	Msa0369100	0.830580	2.710457e-55	-8.615850e-47
Msa0220640	Msa0404070	0.814232	1.710806e-51	-8.615850e-47
Msa0220640	Msa0419040	0.833176	6.198840e-56	-8.615850e-47
Msa0220640	Msa0430040	0.802803	4.718280e-49	-8.615850e-47
Msa0220640	Msa0460220	0.803547	3.308726e-49	-8.615850e-47
Msa0220640	Msa0463150	0.836531	8.861512e-57	-8.615850e-47
Msa0220640	Msa0705810	0.803941	2.741050e-49	-8.615850e-47
Msa0220640	Msa0742090	0.812292	4.561378e-51	-8.615850e-47
Msa0220640	Msa0793100	0.815077	1.112281e-51	-8.615850e-47
Msa0220640	Msa0862230	0.806423	8.286462e-50	-8.615850e-47
Msa0220640	Msa0901660	0.823611	1.262082e-53	-8.615850e-47
Msa0220640	Msa0913120	0.800820	1.204535e-48	-8.615850e-47
Msa0220640	Msa0930950	0.813015	3.170415e-51	-8.615850e-47
Msa0220640	Msa1044790	0.821772	3.379302e-53	-8.615850e-47
Msa0220640	Msa1076280	-0.802607	5.177901e-49	-8.615850e-47
Msa0220640	Msa1109500	0.804420	2.178774e-49	-8.615850e-47
Msa0220640	Msa1133440	0.805638	1.211804e-49	-8.615850e-47
Msa0220640	Msa1184010	0.807214	5.637226e-50	-8.615850e-47
Msa0220640	Msa1211260	0.807796	4.242738e-50	-8.615850e-47
Msa0220640	Msa1228350	0.812314	4.513335e-51	-8.615850e-47
Msa0220640	Msa1228460	0.818524	1.874067e-52	-8.615850e-47
Msa0220640	Msa1299370	0.813135	2.983047e-51	-8.615850e-47
Msa0220640	Msa1349850	0.811715	6.094142e-51	-8.615850e-47
Msa0220640	Msa1369180	0.801168	1.022657e-48	-8.615850e-47
Msa0220640	Msa1375780	0.806946	6.424718e-50	-8.615850e-47
Msa0220640	Msa1395580	0.802633	5.113661e-49	-8.615850e-47
Msa0220640	Msa1405380	0.824319	8.607048e-54	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0220640	MtrunA17_Chr2g0324291	93.567	171	3	2	1	171	1	163	2.35e-115	323
Msa0220640	MtrunA17_Chr4g0021321	59.322	118	44	1	24	137	34	151	6.14e-47	153
Msa0220640	MtrunA17_Chr2g0324281	60.465	129	31	4	19	137	3	121	1.91e-45	148
Msa0220640	MtrunA17_Chr2g0324261	64.545	110	29	2	31	130	37	146	3.59e-45	149
Msa0220640	MtrunA17_Chr2g0282171	53.636	110	45	3	31	135	24	132	7.48e-33	117
Msa0220640	MtrunA17_Chr8g0360011	59.551	89	34	1	31	117	32	120	1.15e-32	116
Msa0220640	MtrunA17_Chr4g0047371	38.667	150	77	5	6	145	7	151	9.71e-30	107
Msa0220640	MtrunA17_Chr4g0047381	48.544	103	47	2	21	117	18	120	4.30e-29	107
Msa0220640	MtrunA17_Chr5g0403521	48.889	90	41	2	32	121	33	117	1.66e-28	105
Msa0220640	MtrunA17_Chr1g0154561	48.352	91	42	2	31	121	29	114	1.23e-27	103
Msa0220640	MtrunA17_Chr1g0175871	36.585	123	63	4	3	117	28	143	2.30e-23	91.3
Msa0220640	MtrunA17_Chr1g0175571	35.294	136	74	5	2	130	86	214	1.14e-22	90.5
Msa0220640	MtrunA17_Chr7g0258821	43.182	88	43	2	34	121	170	250	1.53e-22	93.2
Msa0220640	MtrunA17_Chr1g0175641	42.857	84	44	2	34	117	76	155	2.44e-21	85.9
Msa0220640	MtrunA17_Chr2g0308681	38.889	90	51	1	32	121	105	190	1.54e-19	82.4
Msa0220640	MtrunA17_Chr1g0150921	44.068	59	26	1	35	93	365	416	1.67e-12	64.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0220640	AT4G03510.3	56.989	93	31	2	32	120	44	131	2.73e-33	119
Msa0220640	AT4G03510.1	56.989	93	31	2	32	120	44	131	2.73e-33	119
Msa0220640	AT4G03510.2	56.989	93	31	2	32	120	44	131	2.73e-33	119
Msa0220640	AT4G03510.4	56.989	93	31	2	32	120	44	131	2.73e-33	119
Msa0220640	AT4G28270.1	48.305	118	43	5	31	139	16	124	3.65e-32	114
Msa0220640	AT1G74990.1	46.667	105	47	3	31	135	14	109	4.64e-29	104
Msa0220640	AT2G23780.3	46.465	99	47	3	23	121	16	108	2.85e-27	102
Msa0220640	AT2G23780.2	46.465	99	47	3	23	121	16	108	2.85e-27	102
Msa0220640	AT2G23780.1	46.465	99	47	3	23	121	16	108	2.85e-27	102
Msa0220640	AT1G19310.1	51.136	88	38	2	34	121	21	103	9.50e-27	101
Msa0220640	AT4G27470.1	48.598	107	46	5	34	133	42	146	1.99e-26	100
Msa0220640	AT2G42030.1	46.067	89	40	2	34	122	139	219	6.09e-22	91.7
Msa0220640	AT3G58030.4	44.706	85	39	2	12	96	118	194	1.20e-21	90.9
Msa0220640	AT3G58030.2	44.706	85	39	2	12	96	118	194	1.20e-21	90.9
Msa0220640	AT3G58030.1	44.706	85	39	2	12	96	118	194	1.20e-21	90.9
Msa0220640	AT3G58030.3	44.706	85	39	2	12	96	118	194	1.20e-21	90.9
Msa0220640	AT2G44410.1	37.500	88	51	1	34	121	123	206	7.80e-18	80.1

Find 61 sgRNAs with CRISPR-Local

Find 185 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATTAGTTATGTTCAAGTTTA+AGG	0.077508	2_1:+69060646	None:intergenic
GCAAATCAGAATTGTATAAT+TGG	0.168253	2_1:+69061112	None:intergenic
TTCCGGAAATCAAGCTTATA+TGG	0.186494	2_1:+69059471	None:intergenic
ATGCATTCTGCTGCCAATTA+AGG	0.210654	2_1:-69061039	Msa0220640:five_prime_UTR
GAAATTTCTGATTTGCATAT+TGG	0.222866	2_1:+69059723	None:intergenic
ATGATATTCAATCAAATTTC+CGG	0.230037	2_1:+69059454	None:intergenic
GATTTGCATATTGGACATTC+TGG	0.274453	2_1:+69059732	None:intergenic
GCTTCTGCTTGTTCTAGTTC+AGG	0.300850	2_1:+69059564	None:intergenic
TTGCACATTTGCTTACAATA+TGG	0.306524	2_1:-69059495	Msa0220640:CDS
GCCCATATAAGCTTGATTTC+CGG	0.309807	2_1:-69059473	Msa0220640:CDS
TCAACACTTGTTCCACTATA+TGG	0.312394	2_1:-69059696	Msa0220640:CDS
CAAATCAGAATTGTATAATT+GGG	0.350457	2_1:+69061113	None:intergenic
GCACCATATGCTAGTGCTAA+TGG	0.364544	2_1:-69059961	Msa0220640:exon
AATTGTATAATTGGGGTGGG+GGG	0.367699	2_1:+69061121	None:intergenic
AGCAGAAGCACCACCAGCAC+AGG	0.376264	2_1:-69059526	Msa0220640:CDS
ATTGTATAATTGGGGTGGGG+GGG	0.397306	2_1:+69061122	None:intergenic
AAGAGGTAACTTGGACATAA+AGG	0.400504	2_1:-69060754	Msa0220640:five_prime_UTR
TTTGTGGTCATCTCTATTGT+TGG	0.402478	2_1:-69059806	Msa0220640:CDS
TAATGGACATTCTGTCGAAC+CGG	0.412747	2_1:-69059944	Msa0220640:CDS
CGCTTTAGCATGTATTGAAT+CGG	0.416757	2_1:-69059408	Msa0220640:three_prime_UTR
TTCCAACACAAGAGGTAACT+TGG	0.424249	2_1:-69060763	Msa0220640:five_prime_UTR
GAATTGTATAATTGGGGTGG+GGG	0.435784	2_1:+69061120	None:intergenic
GTTGGCCTTGTATTTACAAA+TGG	0.435974	2_1:-69059788	Msa0220640:CDS
TCCGGAAATCAAGCTTATAT+GGG	0.455288	2_1:+69059472	None:intergenic
GTTGCAGTATCAAGTGATCT+TGG	0.469495	2_1:+69059597	None:intergenic
GATCCAGTGGTAACACTTTG+TGG	0.471364	2_1:-69059822	Msa0220640:CDS
GAAAGTTAGTTGTGTGATGA+AGG	0.480952	2_1:+69060990	None:intergenic
GATCTTGGACCAAGTGGTCT+TGG	0.481662	2_1:+69059612	None:intergenic
AGAATTGTATAATTGGGGTG+GGG	0.483331	2_1:+69061119	None:intergenic
AAGTGATGGTGATGATGTGC+AGG	0.486214	2_1:+69060964	None:intergenic
GTCCAAGTTACCTCTTGTGT+TGG	0.487804	2_1:+69060761	None:intergenic
GTTTCACCACGACCATATAG+TGG	0.492063	2_1:+69059684	None:intergenic
GAAGGTGAGGCTCATCAAGA+TGG	0.515188	2_1:-69059648	Msa0220640:CDS
AATGGACATTCTGTCGAACC+GGG	0.527440	2_1:-69059943	Msa0220640:CDS
TGTCCATTAGCACTAGCATA+TGG	0.529146	2_1:+69059958	None:intergenic
TTAATCCATTTGTAAATACA+AGG	0.530718	2_1:+69059783	None:intergenic
CAGAATTGTATAATTGGGGT+GGG	0.533357	2_1:+69061118	None:intergenic
CTTGGACCAAGTGGTCTTGG+TGG	0.541843	2_1:+69059615	None:intergenic
CTCACCTTCAGAACTTGATG+TGG	0.546869	2_1:+69059662	None:intergenic
AATGGATTAATAGCACCTCA+TGG	0.547502	2_1:-69059770	Msa0220640:CDS
CATTAGCATCAGCTTTGCCC+CGG	0.566386	2_1:+69059925	None:intergenic
TGCAAACTACTCTCCTGTGC+TGG	0.567244	2_1:+69059513	None:intergenic
AAGGTGAGGCTCATCAAGAT+GGG	0.576426	2_1:-69059647	Msa0220640:CDS
TCAGAATTGTATAATTGGGG+TGG	0.577261	2_1:+69061117	None:intergenic
CACATCAAGTTCTGAAGGTG+AGG	0.577593	2_1:-69059661	Msa0220640:CDS
ACCGTGCTGTTACAAGCTGA+AGG	0.578295	2_1:+69060503	None:intergenic
CTTACATAGCTAAGAAGTGA+TGG	0.578724	2_1:+69060950	None:intergenic
AAACTACTCTCCTGTGCTGG+TGG	0.580631	2_1:+69059516	None:intergenic
GTTGTACCACCAAGACCACT+TGG	0.583553	2_1:-69059621	Msa0220640:CDS
AAATCAGAATTGTATAATTG+GGG	0.592797	2_1:+69061114	None:intergenic
ATGGATTAATAGCACCTCAT+GGG	0.600842	2_1:-69059769	Msa0220640:CDS
TGACCACAAAGTGTTACCAC+TGG	0.610639	2_1:+69059819	None:intergenic
GAAACCACATCAAGTTCTGA+AGG	0.614193	2_1:-69059666	Msa0220640:CDS
CTTGTTCCACTATATGGTCG+TGG	0.622216	2_1:-69059690	Msa0220640:CDS
CCCTTCAGCTTGTAACAGCA+CGG	0.625188	2_1:-69060504	Msa0220640:intron
TTTCTCATTATGTTCCCATG+AGG	0.630818	2_1:+69059755	None:intergenic
CCGTGCTGTTACAAGCTGAA+GGG	0.637119	2_1:+69060504	None:intergenic
TCAAGTGATCTTGGACCAAG+TGG	0.644730	2_1:+69059606	None:intergenic
GGAGCATGTGAAAGATCCAG+TGG	0.680623	2_1:-69059835	Msa0220640:CDS
AGTGATGGTGATGATGTGCA+GGG	0.694895	2_1:+69060965	None:intergenic
ATGGACATTCTGTCGAACCG+GGG	0.697290	2_1:-69059942	Msa0220640:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	ATCTTTTTCAGAAAAAATAA+AGG	+	chr2_1:69060112-69060131	None:intergenic	15.0%
!!	AGAGAAAATACTAATATTTA+GGG	-	chr2_1:69060308-69060327	Msa0220640:intron	15.0%
!!!	ATCTTTTTCAGAAAAAATAA+AGG	+	chr2_1:69060112-69060131	None:intergenic	15.0%
!!	AGAGAAAATACTAATATTTA+GGG	-	chr2_1:69060308-69060327	Msa0220640:intron	15.0%
!!	AAATCAGAATTGTATAATTG+GGG	+	chr2_1:69059428-69059447	None:intergenic	20.0%
!!	CAAATCAGAATTGTATAATT+GGG	+	chr2_1:69059429-69059448	None:intergenic	20.0%
!!!	TGCAAGTTTTTTACATAAAA+AGG	-	chr2_1:69059836-69059855	Msa0220640:CDS	20.0%
!!!	ATTAGTTATGTTCAAGTTTA+AGG	+	chr2_1:69059896-69059915	None:intergenic	20.0%
!!!	TTTTATTGAAAATGAAGCAA+AGG	-	chr2_1:69060076-69060095	Msa0220640:intron	20.0%
!!	ATGAATGAAACTTCATTTAT+TGG	-	chr2_1:69060171-69060190	Msa0220640:intron	20.0%
!!!	AAATCATTCAAAGATTTTGA+AGG	-	chr2_1:69060218-69060237	Msa0220640:intron	20.0%
!!	CAGAGAAAATACTAATATTT+AGG	-	chr2_1:69060307-69060326	Msa0220640:intron	20.0%
!!!	ACTTTTTTTAAGTTTCAAGT+TGG	-	chr2_1:69060349-69060368	Msa0220640:intron	20.0%
!!!	CTTTTTTTAAGTTTCAAGTT+GGG	-	chr2_1:69060350-69060369	Msa0220640:intron	20.0%
!!!	TTATGTATGACATTTTAAGT+TGG	-	chr2_1:69060424-69060443	Msa0220640:intron	20.0%
!!	TTAATCCATTTGTAAATACA+AGG	+	chr2_1:69060759-69060778	None:intergenic	20.0%
!!	ATGATATTCAATCAAATTTC+CGG	+	chr2_1:69061088-69061107	None:intergenic	20.0%
!!	AAATCAGAATTGTATAATTG+GGG	+	chr2_1:69059428-69059447	None:intergenic	20.0%
!!	CAAATCAGAATTGTATAATT+GGG	+	chr2_1:69059429-69059448	None:intergenic	20.0%
!!!	TGCAAGTTTTTTACATAAAA+AGG	-	chr2_1:69059836-69059855	Msa0220640:CDS	20.0%
!!!	ATTAGTTATGTTCAAGTTTA+AGG	+	chr2_1:69059896-69059915	None:intergenic	20.0%
!!!	TTTTATTGAAAATGAAGCAA+AGG	-	chr2_1:69060076-69060095	Msa0220640:intron	20.0%
!!	ATGAATGAAACTTCATTTAT+TGG	-	chr2_1:69060171-69060190	Msa0220640:intron	20.0%
!!!	AAATCATTCAAAGATTTTGA+AGG	-	chr2_1:69060218-69060237	Msa0220640:intron	20.0%
!!	CAGAGAAAATACTAATATTT+AGG	-	chr2_1:69060307-69060326	Msa0220640:intron	20.0%
!!!	ACTTTTTTTAAGTTTCAAGT+TGG	-	chr2_1:69060349-69060368	Msa0220640:intron	20.0%
!!!	CTTTTTTTAAGTTTCAAGTT+GGG	-	chr2_1:69060350-69060369	Msa0220640:intron	20.0%
!!!	TTATGTATGACATTTTAAGT+TGG	-	chr2_1:69060424-69060443	Msa0220640:intron	20.0%
!!	TTAATCCATTTGTAAATACA+AGG	+	chr2_1:69060759-69060778	None:intergenic	20.0%
!!	ATGATATTCAATCAAATTTC+CGG	+	chr2_1:69061088-69061107	None:intergenic	20.0%
!	GCAAATCAGAATTGTATAAT+TGG	+	chr2_1:69059430-69059449	None:intergenic	25.0%
!	CAAAGTGAAAAATCCTTAAT+TGG	+	chr2_1:69059516-69059535	None:intergenic	25.0%
!!	ATTGTTAACTCGTAAGTTTT+TGG	+	chr2_1:69059701-69059720	None:intergenic	25.0%
!	AAAAACTTACGAGTTAACAA+TGG	-	chr2_1:69059700-69059719	Msa0220640:CDS	25.0%
!	TTACAAACAAATTGTACTTG+TGG	+	chr2_1:69060396-69060415	None:intergenic	25.0%
!	TGTCATAATTCATACCAATT+AGG	+	chr2_1:69060463-69060482	None:intergenic	25.0%
!	TGAATTATGACAAATGTTAG+AGG	-	chr2_1:69060471-69060490	Msa0220640:intron	25.0%
!	GAATTATGACAAATGTTAGA+GGG	-	chr2_1:69060472-69060491	Msa0220640:intron	25.0%
!	AATTATGACAAATGTTAGAG+GGG	-	chr2_1:69060473-69060492	Msa0220640:intron	25.0%
!!	TTTTGAATGCAACATATGTT+TGG	-	chr2_1:69060683-69060702	Msa0220640:five_prime_UTR	25.0%
!!	GAAATTTCTGATTTGCATAT+TGG	+	chr2_1:69060819-69060838	None:intergenic	25.0%
!	GCAAATCAGAATTGTATAAT+TGG	+	chr2_1:69059430-69059449	None:intergenic	25.0%
!	CAAAGTGAAAAATCCTTAAT+TGG	+	chr2_1:69059516-69059535	None:intergenic	25.0%
!!	ATTGTTAACTCGTAAGTTTT+TGG	+	chr2_1:69059701-69059720	None:intergenic	25.0%
!	AAAAACTTACGAGTTAACAA+TGG	-	chr2_1:69059700-69059719	Msa0220640:CDS	25.0%
!	TTACAAACAAATTGTACTTG+TGG	+	chr2_1:69060396-69060415	None:intergenic	25.0%
!	TGTCATAATTCATACCAATT+AGG	+	chr2_1:69060463-69060482	None:intergenic	25.0%
!	TGAATTATGACAAATGTTAG+AGG	-	chr2_1:69060471-69060490	Msa0220640:intron	25.0%
!	GAATTATGACAAATGTTAGA+GGG	-	chr2_1:69060472-69060491	Msa0220640:intron	25.0%
!	AATTATGACAAATGTTAGAG+GGG	-	chr2_1:69060473-69060492	Msa0220640:intron	25.0%
!!	TTTTGAATGCAACATATGTT+TGG	-	chr2_1:69060683-69060702	Msa0220640:five_prime_UTR	25.0%
!!	GAAATTTCTGATTTGCATAT+TGG	+	chr2_1:69060819-69060838	None:intergenic	25.0%
!!	AAGTTTTTGGAAGCAAACTA+AGG	+	chr2_1:69059688-69059707	None:intergenic	30.0%
	TCTGAAAAAGATCAAAACTC+TGG	-	chr2_1:69060120-69060139	Msa0220640:intron	30.0%
!!!	TGTATGACATTTTAAGTTGG+TGG	-	chr2_1:69060427-69060446	Msa0220640:intron	30.0%
!!!	ATGTTCCATTTTTTGAAGTC+TGG	-	chr2_1:69060515-69060534	Msa0220640:five_prime_UTR	30.0%
!	CAGTTTTACCTTTCAAATTC+AGG	-	chr2_1:69060556-69060575	Msa0220640:five_prime_UTR	30.0%
	TTGCACATTTGCTTACAATA+TGG	-	chr2_1:69061044-69061063	Msa0220640:five_prime_UTR	30.0%
!!	AAGTTTTTGGAAGCAAACTA+AGG	+	chr2_1:69059688-69059707	None:intergenic	30.0%
	TCTGAAAAAGATCAAAACTC+TGG	-	chr2_1:69060120-69060139	Msa0220640:intron	30.0%
!!!	TGTATGACATTTTAAGTTGG+TGG	-	chr2_1:69060427-69060446	Msa0220640:intron	30.0%
!!!	ATGTTCCATTTTTTGAAGTC+TGG	-	chr2_1:69060515-69060534	Msa0220640:five_prime_UTR	30.0%
!	CAGTTTTACCTTTCAAATTC+AGG	-	chr2_1:69060556-69060575	Msa0220640:five_prime_UTR	30.0%
	TTGCACATTTGCTTACAATA+TGG	-	chr2_1:69061044-69061063	Msa0220640:five_prime_UTR	30.0%
	AGAATTGTATAATTGGGGTG+GGG	+	chr2_1:69059423-69059442	None:intergenic	35.0%
	CAGAATTGTATAATTGGGGT+GGG	+	chr2_1:69059424-69059443	None:intergenic	35.0%
	TCAGAATTGTATAATTGGGG+TGG	+	chr2_1:69059425-69059444	None:intergenic	35.0%
	GAAAGTTAGTTGTGTGATGA+AGG	+	chr2_1:69059552-69059571	None:intergenic	35.0%
	CTTACATAGCTAAGAAGTGA+TGG	+	chr2_1:69059592-69059611	None:intergenic	35.0%
!!!	AATTTTGGTTCCAACACAAG+AGG	-	chr2_1:69059768-69059787	Msa0220640:CDS	35.0%
	AAGAGGTAACTTGGACATAA+AGG	-	chr2_1:69059785-69059804	Msa0220640:CDS	35.0%
	TTAGAGGGGACAAATTATAC+TGG	-	chr2_1:69060487-69060506	Msa0220640:intron	35.0%
	TAGCTCCAGACTTCAAAAAA+TGG	+	chr2_1:69060523-69060542	None:intergenic	35.0%
!!!	TTTTTGAAGTCTGGAGCTAA+TGG	-	chr2_1:69060524-69060543	Msa0220640:five_prime_UTR	35.0%
	ATATGGTGCCTGAATTTGAA+AGG	+	chr2_1:69060567-69060586	None:intergenic	35.0%
	TTTGTGGTCATCTCTATTGT+TGG	-	chr2_1:69060733-69060752	Msa0220640:five_prime_UTR	35.0%
!!	GTTGGCCTTGTATTTACAAA+TGG	-	chr2_1:69060751-69060770	Msa0220640:five_prime_UTR	35.0%
!!	AATGGATTAATAGCACCTCA+TGG	-	chr2_1:69060769-69060788	Msa0220640:five_prime_UTR	35.0%
!	ATGGATTAATAGCACCTCAT+GGG	-	chr2_1:69060770-69060789	Msa0220640:five_prime_UTR	35.0%
	TTTCTCATTATGTTCCCATG+AGG	+	chr2_1:69060787-69060806	None:intergenic	35.0%
	GATTTGCATATTGGACATTC+TGG	+	chr2_1:69060810-69060829	None:intergenic	35.0%
	TCAACACTTGTTCCACTATA+TGG	-	chr2_1:69060843-69060862	Msa0220640:five_prime_UTR	35.0%
	TCCGGAAATCAAGCTTATAT+GGG	+	chr2_1:69061070-69061089	None:intergenic	35.0%
	TTCCGGAAATCAAGCTTATA+TGG	+	chr2_1:69061071-69061090	None:intergenic	35.0%
	CGCTTTAGCATGTATTGAAT+CGG	-	chr2_1:69061131-69061150	Msa0220640:five_prime_UTR	35.0%
	AGAATTGTATAATTGGGGTG+GGG	+	chr2_1:69059423-69059442	None:intergenic	35.0%
	CAGAATTGTATAATTGGGGT+GGG	+	chr2_1:69059424-69059443	None:intergenic	35.0%
	TCAGAATTGTATAATTGGGG+TGG	+	chr2_1:69059425-69059444	None:intergenic	35.0%
	GAAAGTTAGTTGTGTGATGA+AGG	+	chr2_1:69059552-69059571	None:intergenic	35.0%
	CTTACATAGCTAAGAAGTGA+TGG	+	chr2_1:69059592-69059611	None:intergenic	35.0%
!!!	AATTTTGGTTCCAACACAAG+AGG	-	chr2_1:69059768-69059787	Msa0220640:CDS	35.0%
	AAGAGGTAACTTGGACATAA+AGG	-	chr2_1:69059785-69059804	Msa0220640:CDS	35.0%
	TTAGAGGGGACAAATTATAC+TGG	-	chr2_1:69060487-69060506	Msa0220640:intron	35.0%
	TAGCTCCAGACTTCAAAAAA+TGG	+	chr2_1:69060523-69060542	None:intergenic	35.0%
!!!	TTTTTGAAGTCTGGAGCTAA+TGG	-	chr2_1:69060524-69060543	Msa0220640:five_prime_UTR	35.0%
	ATATGGTGCCTGAATTTGAA+AGG	+	chr2_1:69060567-69060586	None:intergenic	35.0%
	TTTGTGGTCATCTCTATTGT+TGG	-	chr2_1:69060733-69060752	Msa0220640:five_prime_UTR	35.0%
!!	GTTGGCCTTGTATTTACAAA+TGG	-	chr2_1:69060751-69060770	Msa0220640:five_prime_UTR	35.0%
!!	AATGGATTAATAGCACCTCA+TGG	-	chr2_1:69060769-69060788	Msa0220640:five_prime_UTR	35.0%
!	ATGGATTAATAGCACCTCAT+GGG	-	chr2_1:69060770-69060789	Msa0220640:five_prime_UTR	35.0%
	TTTCTCATTATGTTCCCATG+AGG	+	chr2_1:69060787-69060806	None:intergenic	35.0%
	GATTTGCATATTGGACATTC+TGG	+	chr2_1:69060810-69060829	None:intergenic	35.0%
	TCAACACTTGTTCCACTATA+TGG	-	chr2_1:69060843-69060862	Msa0220640:five_prime_UTR	35.0%
	TCCGGAAATCAAGCTTATAT+GGG	+	chr2_1:69061070-69061089	None:intergenic	35.0%
	TTCCGGAAATCAAGCTTATA+TGG	+	chr2_1:69061071-69061090	None:intergenic	35.0%
	AATTGTATAATTGGGGTGGG+GGG	+	chr2_1:69059421-69059440	None:intergenic	40.0%
	GAATTGTATAATTGGGGTGG+GGG	+	chr2_1:69059422-69059441	None:intergenic	40.0%
	ATGCATTCTGCTGCCAATTA+AGG	-	chr2_1:69059500-69059519	Msa0220640:CDS	40.0%
	TTCCAACACAAGAGGTAACT+TGG	-	chr2_1:69059776-69059795	Msa0220640:CDS	40.0%
	TGTCCATTAGCACTAGCATA+TGG	+	chr2_1:69060584-69060603	None:intergenic	40.0%
!	TAATGGACATTCTGTCGAAC+CGG	-	chr2_1:69060595-69060614	Msa0220640:five_prime_UTR	40.0%
!	GAAACCACATCAAGTTCTGA+AGG	-	chr2_1:69060873-69060892	Msa0220640:five_prime_UTR	40.0%
	GTTGCAGTATCAAGTGATCT+TGG	+	chr2_1:69060945-69060964	None:intergenic	40.0%
	GCCCATATAAGCTTGATTTC+CGG	-	chr2_1:69061066-69061085	Msa0220640:five_prime_UTR	40.0%
	AATTGTATAATTGGGGTGGG+GGG	+	chr2_1:69059421-69059440	None:intergenic	40.0%
	GAATTGTATAATTGGGGTGG+GGG	+	chr2_1:69059422-69059441	None:intergenic	40.0%
	ATGCATTCTGCTGCCAATTA+AGG	-	chr2_1:69059500-69059519	Msa0220640:CDS	40.0%
	TTCCAACACAAGAGGTAACT+TGG	-	chr2_1:69059776-69059795	Msa0220640:CDS	40.0%
	TGTCCATTAGCACTAGCATA+TGG	+	chr2_1:69060584-69060603	None:intergenic	40.0%
!	TAATGGACATTCTGTCGAAC+CGG	-	chr2_1:69060595-69060614	Msa0220640:five_prime_UTR	40.0%
!	GAAACCACATCAAGTTCTGA+AGG	-	chr2_1:69060873-69060892	Msa0220640:five_prime_UTR	40.0%
	GTTGCAGTATCAAGTGATCT+TGG	+	chr2_1:69060945-69060964	None:intergenic	40.0%
	GCCCATATAAGCTTGATTTC+CGG	-	chr2_1:69061066-69061085	Msa0220640:five_prime_UTR	40.0%
	ATTGTATAATTGGGGTGGGG+GGG	+	chr2_1:69059420-69059439	None:intergenic	45.0%
!	AGTGATGGTGATGATGTGCA+GGG	+	chr2_1:69059577-69059596	None:intergenic	45.0%
!	AAGTGATGGTGATGATGTGC+AGG	+	chr2_1:69059578-69059597	None:intergenic	45.0%
	GTCCAAGTTACCTCTTGTGT+TGG	+	chr2_1:69059781-69059800	None:intergenic	45.0%
	GCACCATATGCTAGTGCTAA+TGG	-	chr2_1:69060578-69060597	Msa0220640:five_prime_UTR	45.0%
	AATGGACATTCTGTCGAACC+GGG	-	chr2_1:69060596-69060615	Msa0220640:five_prime_UTR	45.0%
	GATCCAGTGGTAACACTTTG+TGG	-	chr2_1:69060717-69060736	Msa0220640:five_prime_UTR	45.0%
!	TGACCACAAAGTGTTACCAC+TGG	+	chr2_1:69060723-69060742	None:intergenic	45.0%
	CTTGTTCCACTATATGGTCG+TGG	-	chr2_1:69060849-69060868	Msa0220640:five_prime_UTR	45.0%
	GTTTCACCACGACCATATAG+TGG	+	chr2_1:69060858-69060877	None:intergenic	45.0%
	CTCACCTTCAGAACTTGATG+TGG	+	chr2_1:69060880-69060899	None:intergenic	45.0%
!!	CACATCAAGTTCTGAAGGTG+AGG	-	chr2_1:69060878-69060897	Msa0220640:five_prime_UTR	45.0%
	AAGGTGAGGCTCATCAAGAT+GGG	-	chr2_1:69060892-69060911	Msa0220640:five_prime_UTR	45.0%
	TCAAGTGATCTTGGACCAAG+TGG	+	chr2_1:69060936-69060955	None:intergenic	45.0%
!!	GCTTCTGCTTGTTCTAGTTC+AGG	+	chr2_1:69060978-69060997	None:intergenic	45.0%
	ATTGTATAATTGGGGTGGGG+GGG	+	chr2_1:69059420-69059439	None:intergenic	45.0%
!	AGTGATGGTGATGATGTGCA+GGG	+	chr2_1:69059577-69059596	None:intergenic	45.0%
!	AAGTGATGGTGATGATGTGC+AGG	+	chr2_1:69059578-69059597	None:intergenic	45.0%
	GTCCAAGTTACCTCTTGTGT+TGG	+	chr2_1:69059781-69059800	None:intergenic	45.0%
	GCACCATATGCTAGTGCTAA+TGG	-	chr2_1:69060578-69060597	Msa0220640:five_prime_UTR	45.0%
	AATGGACATTCTGTCGAACC+GGG	-	chr2_1:69060596-69060615	Msa0220640:five_prime_UTR	45.0%
	GATCCAGTGGTAACACTTTG+TGG	-	chr2_1:69060717-69060736	Msa0220640:five_prime_UTR	45.0%
!	TGACCACAAAGTGTTACCAC+TGG	+	chr2_1:69060723-69060742	None:intergenic	45.0%
	CTTGTTCCACTATATGGTCG+TGG	-	chr2_1:69060849-69060868	Msa0220640:five_prime_UTR	45.0%
	GTTTCACCACGACCATATAG+TGG	+	chr2_1:69060858-69060877	None:intergenic	45.0%
	CTCACCTTCAGAACTTGATG+TGG	+	chr2_1:69060880-69060899	None:intergenic	45.0%
!!	CACATCAAGTTCTGAAGGTG+AGG	-	chr2_1:69060878-69060897	Msa0220640:five_prime_UTR	45.0%
	AAGGTGAGGCTCATCAAGAT+GGG	-	chr2_1:69060892-69060911	Msa0220640:five_prime_UTR	45.0%
	TCAAGTGATCTTGGACCAAG+TGG	+	chr2_1:69060936-69060955	None:intergenic	45.0%
!!	GCTTCTGCTTGTTCTAGTTC+AGG	+	chr2_1:69060978-69060997	None:intergenic	45.0%
!!!	TAAAAATAAAGATTAAATTT+TGG	-	chr2_1:69059753-69059772	Msa0220640:CDS	5.0%
!!!	TAAAAATAAAGATTAAATTT+TGG	-	chr2_1:69059753-69059772	Msa0220640:CDS	5.0%
!	CCGTGCTGTTACAAGCTGAA+GGG	+	chr2_1:69060038-69060057	None:intergenic	50.0%
	CCCTTCAGCTTGTAACAGCA+CGG	-	chr2_1:69060035-69060054	Msa0220640:intron	50.0%
!	ACCGTGCTGTTACAAGCTGA+AGG	+	chr2_1:69060039-69060058	None:intergenic	50.0%
	GTGGCATTGTGCTGCCTAAT+TGG	-	chr2_1:69060446-69060465	Msa0220640:intron	50.0%
	ATGGACATTCTGTCGAACCG+GGG	-	chr2_1:69060597-69060616	Msa0220640:five_prime_UTR	50.0%
	CATTAGCATCAGCTTTGCCC+CGG	+	chr2_1:69060617-69060636	None:intergenic	50.0%
	GGAGCATGTGAAAGATCCAG+TGG	-	chr2_1:69060704-69060723	Msa0220640:five_prime_UTR	50.0%
	GAAGGTGAGGCTCATCAAGA+TGG	-	chr2_1:69060891-69060910	Msa0220640:five_prime_UTR	50.0%
	GTTGTACCACCAAGACCACT+TGG	-	chr2_1:69060918-69060937	Msa0220640:five_prime_UTR	50.0%
	GATCTTGGACCAAGTGGTCT+TGG	+	chr2_1:69060930-69060949	None:intergenic	50.0%
	AAACTACTCTCCTGTGCTGG+TGG	+	chr2_1:69061026-69061045	None:intergenic	50.0%
	TGCAAACTACTCTCCTGTGC+TGG	+	chr2_1:69061029-69061048	None:intergenic	50.0%
!	CCGTGCTGTTACAAGCTGAA+GGG	+	chr2_1:69060038-69060057	None:intergenic	50.0%
	CCCTTCAGCTTGTAACAGCA+CGG	-	chr2_1:69060035-69060054	Msa0220640:intron	50.0%
!	ACCGTGCTGTTACAAGCTGA+AGG	+	chr2_1:69060039-69060058	None:intergenic	50.0%
	GTGGCATTGTGCTGCCTAAT+TGG	-	chr2_1:69060446-69060465	Msa0220640:intron	50.0%
	ATGGACATTCTGTCGAACCG+GGG	-	chr2_1:69060597-69060616	Msa0220640:five_prime_UTR	50.0%
	CATTAGCATCAGCTTTGCCC+CGG	+	chr2_1:69060617-69060636	None:intergenic	50.0%
	GGAGCATGTGAAAGATCCAG+TGG	-	chr2_1:69060704-69060723	Msa0220640:five_prime_UTR	50.0%
	GAAGGTGAGGCTCATCAAGA+TGG	-	chr2_1:69060891-69060910	Msa0220640:five_prime_UTR	50.0%
	GTTGTACCACCAAGACCACT+TGG	-	chr2_1:69060918-69060937	Msa0220640:five_prime_UTR	50.0%
	GATCTTGGACCAAGTGGTCT+TGG	+	chr2_1:69060930-69060949	None:intergenic	50.0%
	AAACTACTCTCCTGTGCTGG+TGG	+	chr2_1:69061026-69061045	None:intergenic	50.0%
	TGCAAACTACTCTCCTGTGC+TGG	+	chr2_1:69061029-69061048	None:intergenic	50.0%
!	CTTGGACCAAGTGGTCTTGG+TGG	+	chr2_1:69060927-69060946	None:intergenic	55.0%
!	CTTGGACCAAGTGGTCTTGG+TGG	+	chr2_1:69060927-69060946	None:intergenic	55.0%
!!	AGCAGAAGCACCACCAGCAC+AGG	-	chr2_1:69061013-69061032	Msa0220640:five_prime_UTR	60.0%
!!	AGCAGAAGCACCACCAGCAC+AGG	-	chr2_1:69061013-69061032	Msa0220640:five_prime_UTR	60.0%
	TTGGGGTGGGGGGGTGAAGC+AGG	+	chr2_1:69059411-69059430	None:intergenic	70.0%
	TTGGGGTGGGGGGGTGAAGC+AGG	+	chr2_1:69059411-69059430	None:intergenic	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr2_1	gene	69059408	69061153	69059408	ID=Msa0220640;Name=Msa0220640
chr2_1	mRNA	69059408	69061153	69059408	ID=Msa0220640-mRNA-1;Parent=Msa0220640;Name=Msa0220640-mRNA-1;_AED=0.14;_eAED=0.14;_QI=656\|1\|1\|1\|1\|1\|2\|53\|171
chr2_1	exon	69059408	69059983	69059408	ID=Msa0220640-mRNA-1:exon:19521;Parent=Msa0220640-mRNA-1
chr2_1	exon	69060505	69061153	69060505	ID=Msa0220640-mRNA-1:exon:19520;Parent=Msa0220640-mRNA-1
chr2_1	five_prime_UTR	69060505	69061153	69060505	ID=Msa0220640-mRNA-1:five_prime_utr;Parent=Msa0220640-mRNA-1
chr2_1	five_prime_UTR	69059977	69059983	69059977	ID=Msa0220640-mRNA-1:five_prime_utr;Parent=Msa0220640-mRNA-1
chr2_1	CDS	69059461	69059976	69059461	ID=Msa0220640-mRNA-1:cds;Parent=Msa0220640-mRNA-1
chr2_1	three_prime_UTR	69059408	69059460	69059408	ID=Msa0220640-mRNA-1:three_prime_utr;Parent=Msa0220640-mRNA-1

Gene Sequence

>Msa0220640

ATGCTAGTGCTAATGGACATTCTGTCGAACCGGGGCAAAGCTGATGCTAATGCTAATGCTAATGCTAATGCAGATTCTAATAAAATCTGTTCTAATGATTTTGAATGCAACATATGTTTGGAGCATGTGAAAGATCCAGTGGTAACACTTTGTGGTCATCTCTATTGTTGGCCTTGTATTTACAAATGGATTAATAGCACCTCATGGGAACATAATGAGAAACCAGAATGTCCAATATGCAAATCAGAAATTTCAGAATCAACACTTGTTCCACTATATGGTCGTGGTGAAACCACATCAAGTTCTGAAGGTGAGGCTCATCAAGATGGGAATGTTGTACCACCAAGACCACTTGGTCCAAGATCACTTGATACTGCAACTGTTTCACAACCTGAACTAGAACAAGCAGAAGCAGAAGCAGAAGCAGAAGCAGAAGCACCACCAGCACAGGAGAGTAGTTTGCACATTTGCTTACAATATGGCCCATATAAGCTTGATTTCCGGAAATTTGATTGA

Protein sequence

>Msa0220640

MLVLMDILSNRGKADANANANANADSNKICSNDFECNICLEHVKDPVVTLCGHLYCWPCIYKWINSTSWEHNEKPECPICKSEISESTLVPLYGRGETTSSSEGEAHQDGNVVPPRPLGPRSLDTATVSQPELEQAEAEAEAEAEAPPAQESSLHICLQYGPYKLDFRKFD*