AlfalfaGEDB | Msa0526520

M. sativa cultivar zhongmu-4 / Msa0526520

KEGG: K04371|K04464|K20600
GO: GO:0000003 GO:0000165 GO:0000226 GO:0000278 GO:0000280 GO:0000281 GO:0000910 GO:0000911 GO:0001101 GO:0002376 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004707 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005856 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006970 GO:0006971 GO:0006972 GO:0006996 GO:0007010 GO:0007017 GO:0007049 GO:0007112 GO:0007140 GO:0007154 GO:0007165 GO:0007275 GO:0007276 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009504 GO:0009555 GO:0009605 GO:0009607 GO:0009611 GO:0009620 GO:0009627 GO:0009628 GO:0009651 GO:0009719 GO:0009725 GO:0009737 GO:0009751 GO:0009753 GO:0009755 GO:0009814 GO:0009861 GO:0009862 GO:0009863 GO:0009867 GO:0009868 GO:0009987 GO:0010033 GO:0010468 GO:0014070 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019222 GO:0019538 GO:0019953 GO:0022402 GO:0022412 GO:0022414 GO:0023014 GO:0023052 GO:0030865 GO:0031122 GO:0032260 GO:0032501 GO:0032502 GO:0032504 GO:0032506 GO:0032870 GO:0033206 GO:0033993 GO:0035556 GO:0035690 GO:0035821 GO:0036211 GO:0042221 GO:0042493 GO:0042539 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043412 GO:0043622 GO:0044003 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044403 GO:0044419 GO:0044424 GO:0044444 GO:0044464 GO:0044703 GO:0045087 GO:0046677 GO:0048229 GO:0048232 GO:0048285 GO:0048609 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051301 GO:0051321 GO:0051701 GO:0051704 GO:0051707 GO:0051716 GO:0051817 GO:0052031 GO:0052167 GO:0052169 GO:0052173 GO:0052200 GO:0052255 GO:0052306 GO:0052308 GO:0052552 GO:0052553 GO:0052564 GO:0052572 GO:0060255 GO:0061640 GO:0065007 GO:0070887 GO:0071229 GO:0071236 GO:0071310 GO:0071395 GO:0071407 GO:0071446 GO:0071495 GO:0071704 GO:0071840 GO:0075136 GO:0097305 GO:0097435 GO:0098542 GO:0140013 GO:0140096 GO:1901564 GO:1901700 GO:1901701 GO:1902410 GO:1903046 GO:1903047
PFAM: PF00069.28

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0526520	XP_013447093.1	99.482	386	0	1	1	384	1	386	0.0	800

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0526520	sp\|Q39024\|MPK4_ARATH	79.784	371	75	0	9	379	2	372	0.0	641

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0526520	A0A072U5I3	99.482	386	0	1	1	384	1	386	0.0	800

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000610	Msa0526520	0.893874	4.183323e-75	-8.615850e-47
Msa0000800	Msa0526520	-0.817337	3.475020e-52	-8.615850e-47
Msa0001040	Msa0526520	0.815746	7.892021e-52	-8.615850e-47
Msa0001510	Msa0526520	0.809126	2.207816e-50	-8.615850e-47
Msa0002110	Msa0526520	0.901805	1.846992e-78	-8.615850e-47
Msa0002690	Msa0526520	0.872477	3.085249e-67	-8.615850e-47
Msa0002700	Msa0526520	0.874698	5.511188e-68	-8.615850e-47
Msa0003340	Msa0526520	0.846494	2.097401e-59	-8.615850e-47
Msa0003450	Msa0526520	0.811947	5.425521e-51	-8.615850e-47
Msa0004180	Msa0526520	0.861135	1.269256e-63	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0526520	MtrunA17_Chr8g0387401	99.482	386	0	1	1	384	1	386	0.0	800
Msa0526520	MtrunA17_Chr5g0397261	83.149	362	61	0	23	384	14	375	0.0	642
Msa0526520	MtrunA17_Chr7g0248461	80.163	368	73	0	14	381	2	369	0.0	639
Msa0526520	MtrunA17_Chr8g0348481	80.220	364	72	0	18	381	5	368	0.0	639
Msa0526520	MtrunA17_Chr4g0030231	76.836	354	82	0	24	377	14	367	0.0	578
Msa0526520	MtrunA17_Chr4g0045841	68.883	376	116	1	5	380	10	384	0.0	563
Msa0526520	MtrunA17_Chr4g0030151	64.674	368	130	0	13	380	2	369	0.0	529
Msa0526520	MtrunA17_Chr3g0141871	57.550	351	145	4	31	380	15	362	1.01e-147	422
Msa0526520	MtrunA17_Chr8g0378831	55.271	351	153	4	31	380	15	362	1.56e-139	402
Msa0526520	MtrunA17_Chr3g0102571	45.592	397	155	7	36	378	19	408	5.39e-119	351

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	bit score
Msa0526520	AT4G01370.1	79.784	371	75	0	9	379	2	372	641
Msa0526520	AT1G01560.2	80.055	361	72	0	19	379	9	369	616
Msa0526520	AT4G11330.1	76.359	368	87	0	14	381	7	374	606
Msa0526520	AT2G46070.3	76.536	358	84	0	23	380	48	405	590
Msa0526520	AT2G46070.1	76.536	358	84	0	23	380	14	371	589
Msa0526520	AT1G07880.2	72.727	352	96	0	26	377	9	360	555
Msa0526520	AT1G01560.3	77.946	331	68	3	19	344	9	339	530
Msa0526520	AT2G43790.1	65.789	380	129	1	1	380	14	392	530
Msa0526520	AT3G45640.1	64.903	359	126	0	21	379	9	367	515
Msa0526520	AT2G46070.2	77.097	310	71	0	23	332	14	323	513

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Find 304 sgRNAs with CRISPR-Local

Find 430 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACTGCTGTTGGAAGAGG+AGG	0.561022	3_4:+80427928	Msa0526530:CDS
AAAAGGGTTCTTACTCATGG+TGG	0.629306	3_4:-80432131	Msa0526520:CDS
AAACCATGAAATGGGCATTT+TGG	0.233607	3_4:+80427989	Msa0526530:CDS
AAAGCAATCACAGCGCTGGT+TGG	0.571934	3_4:-80427823	Msa0526520:three_prime_UTR
AAAGTTTAAAGATAATTATC+GGG	0.501670	3_4:+80428964	None:intergenic
AAATATGATTTGTGGTTGGG+TGG	0.560229	3_4:+80426262	Msa0526530:five_prime_UTR
AAATCAAAAGAGAAAGGCCT+AGG	0.513586	3_4:+80429386	None:intergenic
AACAATTGAGGAGCAATTCC+GGG	0.412046	3_4:+80430093	None:intergenic
AACATGATATGAAAGAGATT+AGG	0.523913	3_4:+80432950	None:intergenic
AACCATTGGATTAGCAAAGA+AGG	0.502657	3_4:+80428179	Msa0526530:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TGCATATGAAATGAAACAAG+TGG	+	chr3_4:80426083-80426102	None:intergenic	30.0%
ATGGAACATGGTTAACTTAT+AGG	-	chr3_4:80426118-80426137	Msa0526520:three_prime_UTR	30.0%
CTTAAGTGTTTGAACCATTA+TGG	-	chr3_4:80426186-80426205	Msa0526520:three_prime_UTR	30.0%
TTCTTGGAACAATGTATTGT+TGG	+	chr3_4:80426467-80426486	Msa0526530:exon	30.0%
ATACATTGTTCCAAGAACAA+AGG	-	chr3_4:80426470-80426489	Msa0526520:three_prime_UTR	30.0%
AGAGATGAACACTTACATTT+GGG	+	chr3_4:80427202-80427221	Msa0526530:CDS	30.0%
AAGAGATGAACACTTACATT+TGG	+	chr3_4:80427203-80427222	Msa0526530:CDS	30.0%
ATTAATTAACGTGGCAATAG+TGG	-	chr3_4:80427300-80427319	Msa0526520:three_prime_UTR	30.0%
TAAATCCGACCATTGTAAAA+TGG	+	chr3_4:80427326-80427345	Msa0526530:CDS	30.0%
AATTTAGCTGTATACACTCA+TGG	+	chr3_4:80427450-80427469	Msa0526530:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr3_4	gene	80425979	80432979	80425979	ID=Msa0526520;Name=Msa0526520
chr3_4	mRNA	80425979	80432979	80425979	ID=Msa0526520-mRNA-1;Parent=Msa0526520;Name=Msa0526520-mRNA-1;_AED=0.05;_eAED=0.05;_QI=547\|1\|1\|1\|0.62\|0.44\|9\|3052\|384
chr3_4	exon	80425979	80426809	80425979	ID=Msa0526520-mRNA-1:exon:24185;Parent=Msa0526520-mRNA-1
chr3_4	exon	80426922	80427521	80426922	ID=Msa0526520-mRNA-1:exon:24184;Parent=Msa0526520-mRNA-1
chr3_4	exon	80427677	80429473	80427677	ID=Msa0526520-mRNA-1:exon:24183;Parent=Msa0526520-mRNA-1
chr3_4	exon	80429566	80429749	80429566	ID=Msa0526520-mRNA-1:exon:24182;Parent=Msa0526520-mRNA-1
chr3_4	exon	80429974	80430306	80429974	ID=Msa0526520-mRNA-1:exon:24181;Parent=Msa0526520-mRNA-1
chr3_4	exon	80430779	80430916	80430779	ID=Msa0526520-mRNA-1:exon:24180;Parent=Msa0526520-mRNA-1
chr3_4	exon	80431753	80431882	80431753	ID=Msa0526520-mRNA-1:exon:24179;Parent=Msa0526520-mRNA-1
chr3_4	exon	80432029	80432256	80432029	ID=Msa0526520-mRNA-1:exon:24178;Parent=Msa0526520-mRNA-1
chr3_4	exon	80432467	80432979	80432467	ID=Msa0526520-mRNA-1:exon:24177;Parent=Msa0526520-mRNA-1
chr3_4	five_prime_UTR	80432467	80432979	80432467	ID=Msa0526520-mRNA-1:five_prime_utr;Parent=Msa0526520-mRNA-1
chr3_4	five_prime_UTR	80432223	80432256	80432223	ID=Msa0526520-mRNA-1:five_prime_utr;Parent=Msa0526520-mRNA-1
chr3_4	CDS	80432029	80432222	80432029	ID=Msa0526520-mRNA-1:cds;Parent=Msa0526520-mRNA-1
chr3_4	CDS	80431753	80431882	80431753	ID=Msa0526520-mRNA-1:cds;Parent=Msa0526520-mRNA-1
chr3_4	CDS	80430779	80430916	80430779	ID=Msa0526520-mRNA-1:cds;Parent=Msa0526520-mRNA-1
chr3_4	CDS	80429974	80430306	80429974	ID=Msa0526520-mRNA-1:cds;Parent=Msa0526520-mRNA-1
chr3_4	CDS	80429566	80429749	80429566	ID=Msa0526520-mRNA-1:cds;Parent=Msa0526520-mRNA-1
chr3_4	CDS	80429298	80429473	80429298	ID=Msa0526520-mRNA-1:cds;Parent=Msa0526520-mRNA-1
chr3_4	three_prime_UTR	80427677	80429297	80427677	ID=Msa0526520-mRNA-1:three_prime_utr;Parent=Msa0526520-mRNA-1
chr3_4	three_prime_UTR	80426922	80427521	80426922	ID=Msa0526520-mRNA-1:three_prime_utr;Parent=Msa0526520-mRNA-1
chr3_4	three_prime_UTR	80425979	80426809	80425979	ID=Msa0526520-mRNA-1:three_prime_utr;Parent=Msa0526520-mRNA-1

Gene Sequence

>Msa0526520

ATGGCCACAAAAGAGTCAAGCTCTTCTGCTGCTGCTGCTGCTGCTTCAGCTTCAGGGGACACTAAAATCAAAAGGGTTCTTACTCATGGTGGTAAATATGCACACTACAATGTGTATGGAAACTTGTTTGAGGTGTCTTCTAAGTATGTGCCACCCATTCGCCCTATTGGTAGAGGCGCTTATGGTATCGTTTGTGCTGCTGTTAATTCTGATACACATGAACAAGTTGCCATCAAGAAGATTGGTAATGCATTTGACAATATAATTGATGCTAAAAGGACTTTGAGAGAAATCAAGCTCCTTCGTCACATGGATCACCCAAATATCATTGCCATCAAGGATATCATACGACCCCCGAAAAAGGAGGCATTTAATGATGTATACATTGTTTATGAATTGATGGACACTGATCTCCATCATATAATTCATTCTGACCAACCTCTTCGTGAAGAACATTGTCAGTACTTCTTATATCAGCTGTTACGTGGGTTGAAATATGTGCACTCAGCTAATGTTTTGCACCGTGATCTTAAGCCAAGTAATTTACTAGTCAATGCAAACTGTGACCTTAAAATTGGTGACTTCGGTTTGGCAAGGACAACATCCGAAACAGATTTCATGACTGAGTATGTGGTCACAAGATGGTACCGAGCCCCGGAATTGCTCCTCAATTGTTCTGAGTACACTTCTGCAATTGATGTTTGGTCTGTTGGTTGCATATTTGGTGAAATTATGACCAGAGAGCCCTTGTTTCCTGGCAAAGACTATGTTCATCAACTAAGGCTTATCACAGAGTTAATAGGTTCACCTGATGATGCGAGCCTTGGATTTCTCCGAAGTGACAATGCTAGAAGATATTTTAGACAGTTTCAACAGTACCGGAAGCAAAAATTCTCGTCCAGGTTCCCAAACATGTTACCTGAGGCACTTGATCTGTTAGAAAAGATGCTTATCTTTGATCCAAACAAACGCATTACAGTTGATGAGGCACTGTGTCACCCATATCTTTCCTCACTTCACAACACCAATGAGGAGCCAGTTTGTCCTAGGCCTTTCTCTTTTGATTTTGATCAACCTACATGCACTGAAGACAATATCAAGGAGATCATTTGGAAGGAATCTGTGAAGTTCAATCCAGATCCACTTTGTCAGTAA

Protein sequence

>Msa0526520

MATKESSSSAAAAAASASGDTKIKRVLTHGGKYAHYNVYGNLFEVSSKYVPPIRPIGRGAYGIVCAAVNSDTHEQVAIKKIGNAFDNIIDAKRTLREIKLLRHMDHPNIIAIKDIIRPPKKEAFNDVYIVYELMDTDLHHIIHSDQPLREEHCQYFLYQLLRGLKYVHSANVLHRDLKPSNLLVNANCDLKIGDFGLARTTSETDFMTEYVVTRWYRAPELLLNCSEYTSAIDVWSVGCIFGEIMTREPLFPGKDYVHQLRLITELIGSPDDASLGFLRSDNARRYFRQFQQYRKQKFSSRFPNMLPEALDLLEKMLIFDPNKRITVDEALCHPYLSSLHNTNEEPVCPRPFSFDFDQPTCTEDNIKEIIWKESVKFNPDPLCQ*