AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0609660

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0609660 MtrunA17_Chr4g0042481 95.075 467 21 1 1 467 1 465 0.0 894
Msa0609660 MtrunA17_Chr6g0453321 44.071 506 229 18 1 465 1 493 7.15e-115 347
Msa0609660 MtrunA17_Chr7g0217581 43.461 497 236 16 1 465 98 581 8.66e-106 326
Msa0609660 MtrunA17_Chr3g0092111 43.838 495 216 20 1 465 1 463 7.65e-105 320
Msa0609660 MtrunA17_Chr5g0417601 41.237 485 151 17 6 465 4 379 8.84e-87 270
Msa0609660 MtrunA17_Chr5g0417551 38.320 488 151 17 6 465 4 369 1.99e-73 236
Msa0609660 MtrunA17_Chr5g0417561 36.605 489 152 18 6 466 4 362 6.46e-71 229
Msa0609660 MtrunA17_Chr8g0355261 33.537 492 231 19 5 465 11 437 3.76e-62 208
Msa0609660 MtrunA17_Chr7g0254161 32.787 488 237 17 1 465 3 422 4.12e-59 200
Msa0609660 MtrunA17_Chr5g0417571 49.528 212 70 8 4 185 2 206 1.70e-50 174
Msa0609660 MtrunA17_Chr6g0460731 34.056 323 142 11 4 306 9 280 1.32e-42 154
Msa0609660 MtrunA17_Chr6g0460721 46.857 175 76 4 85 243 51 224 8.10e-42 151
Msa0609660 MtrunA17_Chr2g0283811 86.667 60 8 0 116 175 41 100 9.20e-34 124
Msa0609660 MtrunA17_Chr3g0127201 61.765 68 26 0 108 175 13 80 8.85e-26 106
Msa0609660 MtrunA17_Chr8g0346551 65.625 64 22 0 117 180 19 82 2.23e-25 105
Msa0609660 MtrunA17_Chr8g0367161 52.381 84 40 0 97 180 7 90 4.20e-25 105
Msa0609660 MtrunA17_Chr8g0374111 63.934 61 22 0 122 182 29 89 1.01e-24 102
Msa0609660 MtrunA17_Chr2g0326001 64.615 65 23 0 111 175 29 93 2.09e-24 102
Msa0609660 MtrunA17_Chr8g0374251 66.102 59 20 0 122 180 68 126 2.74e-24 103
Msa0609660 MtrunA17_Chr4g0010151 67.241 58 19 0 118 175 78 135 3.10e-24 103
Msa0609660 MtrunA17_Chr1g0188851 57.971 69 29 0 106 174 27 95 7.39e-24 101
Msa0609660 MtrunA17_Chr4g0047001 74.074 54 14 0 124 177 57 110 1.19e-23 102
Msa0609660 MtrunA17_Chr2g0308011 73.469 49 13 0 122 170 37 85 2.83e-23 97.4
Msa0609660 MtrunA17_Chr1g0177251 53.333 90 40 2 117 204 6 95 4.32e-23 99.0
Msa0609660 MtrunA17_Chr4g0031411 58.571 70 26 1 108 174 58 127 4.40e-23 99.8
Msa0609660 MtrunA17_Chr2g0328021 68.519 54 17 0 122 175 62 115 5.14e-23 98.6
Msa0609660 MtrunA17_Chr4g0061761 67.925 53 17 0 120 172 38 90 5.55e-23 99.4
Msa0609660 MtrunA17_Chr8g0341671 62.903 62 23 0 122 183 20 81 6.58e-23 96.7
Msa0609660 MtrunA17_Chr4g0030051 56.000 75 29 1 104 174 19 93 2.56e-22 96.7
Msa0609660 MtrunA17_Chr7g0237921 71.154 52 15 0 124 175 78 129 2.72e-22 97.8
Msa0609660 MtrunA17_Chr7g0223311 72.549 51 14 0 124 174 79 129 6.54e-22 97.1
Msa0609660 MtrunA17_Chr2g0282051 67.857 56 17 1 122 177 46 100 7.34e-22 95.1
Msa0609660 MtrunA17_Chr3g0126201 59.155 71 27 2 107 175 34 104 7.87e-22 96.3
Msa0609660 MtrunA17_Chr4g0047761 70.370 54 15 1 124 177 74 126 1.37e-21 95.1
Msa0609660 MtrunA17_Chr5g0411951 63.636 55 20 0 118 172 39 93 1.47e-21 95.5
Msa0609660 MtrunA17_Chr2g0282511 71.429 49 14 0 122 170 45 93 1.53e-21 95.1
Msa0609660 MtrunA17_Chr3g0117601 61.290 62 23 1 112 172 50 111 2.61e-21 94.7
Msa0609660 MtrunA17_Chr2g0282441 70.000 50 15 0 124 173 27 76 2.87e-21 94.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0609660 AT3G47500.1 40.408 490 224 17 1 466 2 447 7.07e-85 268
Msa0609660 AT5G39660.3 37.403 516 208 17 4 465 1 455 2.69e-80 256
Msa0609660 AT5G39660.1 37.403 516 208 17 4 465 1 455 2.69e-80 256
Msa0609660 AT5G39660.2 37.403 516 208 17 4 465 1 455 2.69e-80 256
Msa0609660 AT5G62430.1 41.361 382 111 15 89 466 25 297 1.45e-64 211
Msa0609660 AT1G69570.1 32.985 479 223 17 1 465 1 395 5.80e-48 170
Msa0609660 AT1G26790.1 32.265 468 210 17 1 465 7 370 3.71e-47 167
Msa0609660 AT1G29160.1 88.333 60 7 0 116 175 56 115 2.44e-35 129
Msa0609660 AT2G34140.1 52.727 110 52 0 66 175 2 111 4.80e-34 126
Msa0609660 AT5G60200.1 37.931 174 99 4 118 288 51 218 2.66e-27 110
Msa0609660 AT4G24060.1 61.538 65 25 0 111 175 42 106 2.66e-24 103
Msa0609660 AT1G51700.1 53.086 81 31 1 100 180 18 91 2.71e-24 100
Msa0609660 AT3G55370.1 43.243 111 49 2 118 217 72 179 3.86e-24 103
Msa0609660 AT3G55370.2 43.243 111 49 2 118 217 72 179 4.56e-24 103
Msa0609660 AT5G60850.1 72.222 54 15 0 122 175 51 104 4.76e-24 102
Msa0609660 AT2G28810.2 46.078 102 49 2 78 173 27 128 5.06e-24 102
Msa0609660 AT3G55370.3 67.241 58 19 0 118 175 117 174 5.85e-24 103
Msa0609660 AT3G50410.1 68.421 57 18 0 122 178 30 86 6.76e-24 100
Msa0609660 AT2G28810.1 46.078 102 49 2 78 173 44 145 7.32e-24 102
Msa0609660 AT1G21340.1 48.936 94 37 3 124 216 40 123 9.39e-24 100
Msa0609660 AT2G37590.1 57.576 66 28 0 110 175 77 142 1.20e-23 101
Msa0609660 AT1G64620.1 55.128 78 34 1 93 170 21 97 1.52e-23 102
Msa0609660 AT3G61850.2 60.938 64 25 0 112 175 52 115 1.83e-23 100
Msa0609660 AT3G61850.3 60.938 64 25 0 112 175 52 115 1.83e-23 100
Msa0609660 AT3G61850.1 58.824 68 28 0 112 179 64 131 2.22e-23 100
Msa0609660 AT3G61850.5 60.938 64 25 0 112 175 52 115 3.15e-23 100
Msa0609660 AT3G61850.4 58.824 68 28 0 112 179 64 131 3.59e-23 100
Msa0609660 AT3G21270.1 71.698 53 15 0 122 174 29 81 3.94e-23 97.4
Msa0609660 AT2G28510.1 67.797 59 19 0 124 182 50 108 6.96e-23 98.6
Msa0609660 AT1G07640.1 69.811 53 16 0 122 174 21 73 7.96e-23 98.2
Msa0609660 AT3G45610.1 57.143 63 27 0 118 180 36 98 8.58e-23 97.4
Msa0609660 AT1G28310.1 70.370 54 16 0 122 175 27 80 9.50e-23 99.0
Msa0609660 AT1G07640.2 69.811 53 16 0 122 174 77 129 1.30e-22 99.0
Msa0609660 AT1G28310.2 47.475 99 43 2 79 175 13 104 1.55e-22 98.6
Msa0609660 AT1G07640.3 69.811 53 16 0 122 174 85 137 1.65e-22 98.6
Msa0609660 AT5G02460.1 57.746 71 27 1 108 175 78 148 1.86e-22 99.4
Msa0609660 AT5G66940.1 60.000 60 24 0 122 181 32 91 2.47e-22 95.5
Msa0609660 AT5G65590.1 54.930 71 32 0 105 175 24 94 3.36e-22 97.4
Msa0609660 AT4G00940.1 56.757 74 27 1 109 177 50 123 4.54e-22 96.7
Msa0609660 AT4G00940.3 56.757 74 27 1 109 177 50 123 4.54e-22 96.7
Msa0609660 AT4G00940.2 56.757 74 27 1 109 177 50 123 4.54e-22 96.7
Msa0609660 AT1G47655.1 71.429 49 14 0 122 170 29 77 7.53e-22 94.0
Msa0609660 AT3G52440.2 48.936 94 40 3 93 180 11 102 9.83e-22 95.1
Msa0609660 AT4G38000.1 62.500 56 20 1 121 176 40 94 2.77e-21 93.2
Msa0609660 AT3G52440.1 64.912 57 20 0 124 180 27 83 2.91e-21 93.2
Msa0609660 AT2G46590.1 60.656 61 22 1 109 169 57 115 3.50e-21 95.1
Msa0609660 AT2G46590.2 60.656 61 22 1 109 169 69 127 3.76e-21 95.1
Msa0609660 AT4G21050.1 43.564 101 55 1 115 213 17 117 6.50e-21 91.3
Msa0609660 AT5G62940.1 69.388 49 15 0 124 172 75 123 9.90e-20 90.9
Msa0609660 AT4G21040.1 47.297 74 37 1 102 175 7 78 1.15e-17 82.4
Msa0609660 AT4G21080.1 57.692 52 22 0 124 175 27 78 1.55e-16 79.7
Msa0609660 AT4G21030.1 59.091 44 18 0 124 167 23 66 4.09e-11 62.8

Find 141 sgRNAs with CRISPR-Local

Find 215 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCAGTTCTGTCCCGACTTT+GGG 0.197222 4_2:-57015111 Msa0609660:CDS
CCCTAATGGATTACATAGTT+TGG 0.197772 4_2:-57015586 Msa0609660:CDS
TTTCAATGCAATTCACAATT+AGG 0.220175 4_2:+57014771 None:intergenic
GTAATTAGTTCTTTCCTTGA+TGG 0.235655 4_2:+57016026 None:intergenic
CCAAACTATGTAATCCATTA+GGG 0.240822 4_2:+57015586 None:intergenic
AGTAACTCCACCTGCATATT+GGG 0.244547 4_2:-57015208 Msa0609660:CDS
TCATCAAATCTAAGTGTCTT+TGG 0.276528 4_2:+57014991 None:intergenic
CAATATGCAGGTGGAGTTAC+TGG 0.285499 4_2:+57015210 None:intergenic
TTTGAGACTAACATTATTGT+AGG 0.298707 4_2:+57014391 None:intergenic
GTAAGAAATGTCAAAGATAC+TGG 0.307217 4_2:-57015725 Msa0609660:CDS
ATTGTTGAAGTAACAAAATT+TGG 0.309323 4_2:+57015776 None:intergenic
CCCAAACTATGTAATCCATT+AGG 0.311917 4_2:+57015585 None:intergenic
ATTCCATGGATAAGGCAAAA+AGG 0.312423 4_2:+57015290 None:intergenic
CAGTAACTCCACCTGCATAT+TGG 0.313645 4_2:-57015209 Msa0609660:CDS
GCTAAACTTAATTCCCCTAA+TGG 0.325757 4_2:-57015600 Msa0609660:CDS
ACAATAATGTTAGTCTTCAT+TGG 0.332215 4_2:-57014452 Msa0609660:three_prime_UTR
GCCTCCTCCATTGAGAGAAT+TGG 0.332869 4_2:+57014909 None:intergenic
CCTAATGGATTACATAGTTT+GGG 0.335592 4_2:-57015585 Msa0609660:CDS
TAGAATGCTAATGGATAGTT+TGG 0.337503 4_2:+57015237 None:intergenic
CTGGACTCTTGCATGGAGTT+TGG 0.349607 4_2:+57014645 None:intergenic
GTTTCAGCTGATGGAGTCTT+AGG 0.353075 4_2:+57015921 None:intergenic
AAGAACTTGTCAGCAGAATA+AGG 0.366367 4_2:+57014702 None:intergenic
ATCATGCAAGTTCGTATAGT+AGG 0.370122 4_2:-57015452 Msa0609660:CDS
TCTCAGTTCTGTCCCGACTT+TGG 0.371870 4_2:-57015112 Msa0609660:CDS
AAAGGCCACACCGGCACAAA+TGG 0.375943 4_2:-57015369 Msa0609660:CDS
GACCCTGCGATAAAGCTCTT+TGG 0.376470 4_2:-57017288 Msa0609660:CDS
GGGGATATTATATAGTTTGA+TGG 0.384459 4_2:+57017470 None:intergenic
ACAAATGGGTTGGTAGATAA+AGG 0.391250 4_2:-57015354 Msa0609660:CDS
AGGGAAAATGATCACTCAAT+TGG 0.410885 4_2:-57015432 Msa0609660:CDS
CATCAAATCTAAGTGTCTTT+GGG 0.411049 4_2:+57014992 None:intergenic
TGATTAACAGAAGCACTTCC+TGG 0.414702 4_2:+57015147 None:intergenic
GAACTCCTTGCAGCTTCACT+TGG 0.415659 4_2:+57014967 None:intergenic
AGAGGGAGATGAAGAGTCAT+TGG 0.419013 4_2:+57017230 None:intergenic
AGATTTCAATAACGAATTCT+CGG 0.425048 4_2:+57015899 None:intergenic
TTGGTAGATAAAGGAGTTGA+AGG 0.425049 4_2:-57015345 Msa0609660:CDS
GGATTACATAGTTTGGGAAA+TGG 0.427090 4_2:-57015579 Msa0609660:CDS
ACTCAAAATTGTGTGTCAAA+TGG 0.434475 4_2:-57015480 Msa0609660:CDS
CAGATTTGATAATATGTTGT+TGG 0.444254 4_2:-57014542 Msa0609660:three_prime_UTR
TGTATTGCAGAGGTGAATTC+TGG 0.445396 4_2:+57017198 None:intergenic
TTGAGCCTGCCTAACTGAAC+AGG 0.455235 4_2:-57014576 Msa0609660:three_prime_UTR
AGTTCATTGTGGTCAAAGCT+TGG 0.456620 4_2:-57014949 Msa0609660:CDS
TTGAGTGTTTCCCCAAAGTC+GGG 0.458025 4_2:+57015100 None:intergenic
ACTGGAGTATAGAATGCTAA+TGG 0.468260 4_2:+57015228 None:intergenic
TGATCTTTATTATTCTCAAA+AGG 0.468272 4_2:-57014680 Msa0609660:three_prime_UTR
GTGATTCATGTCCTTTACCT+TGG 0.468280 4_2:+57014867 None:intergenic
GTTCATTGTGGTCAAAGCTT+GGG 0.469560 4_2:-57014948 Msa0609660:CDS
GACAAAGCCAATTCTCTCAA+TGG 0.470994 4_2:-57014916 Msa0609660:CDS
TGGATAGTTTGGTGGACAAA+AGG 0.471372 4_2:+57015248 None:intergenic
GGAGATGAAGAGTCATTGGC+AGG 0.477484 4_2:+57017234 None:intergenic
AATGGAGGAGGCATGTTCAA+TGG 0.477902 4_2:-57014898 Msa0609660:CDS
GAATTCTCGGTTTCAGCTGA+TGG 0.478162 4_2:+57015912 None:intergenic
ATGCTATTGCATCGCGGGCA+TGG 0.486276 4_2:+57015804 None:intergenic
GAATTCTGGTGGAGAAGAAG+AGG 0.486452 4_2:+57017212 None:intergenic
ATTGCAGAATTCCATGGATA+AGG 0.486834 4_2:+57015282 None:intergenic
GAATTAAGTTTAGCACCCTC+TGG 0.491397 4_2:+57015609 None:intergenic
AGATTTGATAATATGTTGTT+GGG 0.494242 4_2:-57014541 Msa0609660:three_prime_UTR
TTGCGGCGACCAGCTCCAAC+AGG 0.495368 4_2:+57015675 None:intergenic
CCGCGATAAAGCCGCAGGGT+TGG 0.504467 4_2:+57014816 None:intergenic
AATGATCACTCAATTGGAGT+TGG 0.507132 4_2:-57015426 Msa0609660:CDS
AACTCCTTGCAGCTTCACTT+GGG 0.509294 4_2:+57014968 None:intergenic
AAAGTACAACACTGTTGTCC+TGG 0.510065 4_2:+57014626 None:intergenic
TCAAATTCATCAGATAGAAA+AGG 0.510956 4_2:-57015387 Msa0609660:CDS
AAGGCCACACCGGCACAAAT+GGG 0.516822 4_2:-57015368 Msa0609660:CDS
GGCATACAACCCCAATATGC+AGG 0.516977 4_2:+57015198 None:intergenic
AAACACCTGTTCAGTTAGGC+AGG 0.517381 4_2:+57014571 None:intergenic
TGGATTTCAGTCCAAGGTAA+AGG 0.518064 4_2:-57014878 Msa0609660:CDS
CAATTAGGTTTCCTCATGGA+AGG 0.522646 4_2:+57014786 None:intergenic
CTTGAGTGTTTCCCCAAAGT+CGG 0.525777 4_2:+57015099 None:intergenic
TGTCAAAGATACTGGACTGC+AGG 0.528621 4_2:-57015717 Msa0609660:CDS
ATCTACCAACCCATTTGTGC+CGG 0.532135 4_2:+57015359 None:intergenic
TGTCCATGCTATTGCATCGC+GGG 0.535971 4_2:+57015799 None:intergenic
CGAGACCGCGATAAAGCCGC+AGG 0.536771 4_2:+57014811 None:intergenic
TTTACCTCTGACATTTCTGT+AGG 0.537948 4_2:+57017312 None:intergenic
TGTTGTCCTGGACTCTTGCA+TGG 0.538310 4_2:+57014638 None:intergenic
CGACTTTGGGGAAACACTCA+AGG 0.540052 4_2:-57015098 Msa0609660:CDS
TCATGCAAGTTCGTATAGTA+GGG 0.541345 4_2:-57015451 Msa0609660:CDS
TTCACAATTAGGTTTCCTCA+TGG 0.553418 4_2:+57014782 None:intergenic
ATCCTCAGTGGTTTGTTGCG+AGG 0.554021 4_2:+57015986 None:intergenic
AATGCTAATGGATAGTTTGG+TGG 0.554354 4_2:+57015240 None:intergenic
GAGACACATGGTATATTCCA+TGG 0.554398 4_2:+57015171 None:intergenic
GCAGAAACACCTGTTCAGTT+AGG 0.556152 4_2:+57014567 None:intergenic
CTTCCAAAGAGCTTTATCGC+AGG 0.556819 4_2:+57017285 None:intergenic
ACAATGAGAAATGTTCCTGT+TGG 0.557862 4_2:-57015690 Msa0609660:CDS
TCAGCTGATGGAGTCTTAGG+AGG 0.562404 4_2:+57015924 None:intergenic
TAGTTTGGTGGACAAAAGGT+AGG 0.562573 4_2:+57015252 None:intergenic
AATTCTGGTGGAGAAGAAGA+GGG 0.562827 4_2:+57017213 None:intergenic
GCCAATTCTCTCAATGGAGG+AGG 0.563194 4_2:-57014910 Msa0609660:CDS
TTCCAAAGAGCTTTATCGCA+GGG 0.567023 4_2:+57017286 None:intergenic
GAATATGTTGCAACTGAGGA+GGG 0.570048 4_2:+57015319 None:intergenic
GGAGGCATTGCAGAATTCCA+TGG 0.572249 4_2:+57015276 None:intergenic
TTTGGTGGACAAAAGGTAGG+AGG 0.574664 4_2:+57015255 None:intergenic
GGAATATGTTGCAACTGAGG+AGG 0.574863 4_2:+57015318 None:intergenic
TGAGTGAAAATATTATGAGA+TGG 0.574965 4_2:+57017370 None:intergenic
AGAAATGTTCCTGTTGGAGC+TGG 0.575931 4_2:-57015684 Msa0609660:CDS
CTTGGAATATGTTGCAACTG+AGG 0.581444 4_2:+57015315 None:intergenic
TTGGGGAAACACTCAAGGGA+TGG 0.586619 4_2:-57015093 Msa0609660:CDS
GGGGTTGTATGCCACCTCCA+TGG 0.588317 4_2:-57015188 Msa0609660:CDS
CATGGTATATTCCATGGAGG+TGG 0.595774 4_2:+57015177 None:intergenic
GGTGGACAAAAGGTAGGAGG+AGG 0.603448 4_2:+57015258 None:intergenic
CCAACCCATTTGTGCCGGTG+TGG 0.605033 4_2:+57015364 None:intergenic
CAAACTCCATGCAAGAGTCC+AGG 0.606193 4_2:-57014644 Msa0609660:three_prime_UTR
CGACATTTGATAATTCCAGA+GGG 0.606272 4_2:-57015624 Msa0609660:CDS
TCAGTTCTGTCCCGACTTTG+GGG 0.606745 4_2:-57015110 Msa0609660:CDS
CCACACCGGCACAAATGGGT+TGG 0.611096 4_2:-57015364 Msa0609660:CDS
GTAACTCCACCTGCATATTG+GGG 0.611433 4_2:-57015207 Msa0609660:CDS
CAAAGATACTGGACTGCAGG+AGG 0.611856 4_2:-57015714 Msa0609660:CDS
GTTCAATGGATTTCAGTCCA+AGG 0.615097 4_2:-57014884 Msa0609660:CDS
CTGCGATAAAGCTCTTTGGA+AGG 0.619750 4_2:-57017284 Msa0609660:CDS
AATATACCATGTGTCTCACC+AGG 0.620306 4_2:-57015165 Msa0609660:CDS
GATGTTCAATACAGAAGCCA+TGG 0.623380 4_2:+57015512 None:intergenic
GAGATGAAGAGTCATTGGCA+GGG 0.625244 4_2:+57017235 None:intergenic
TCGACATTTGATAATTCCAG+AGG 0.625580 4_2:-57015625 Msa0609660:CDS
TCATTGGCAGGGATGATCAG+TGG 0.630276 4_2:+57017246 None:intergenic
GCACTTCCTGGTGAGACACA+TGG 0.630353 4_2:+57015159 None:intergenic
AGTTTCATGCTGTATTGCAG+AGG 0.631365 4_2:+57017188 None:intergenic
GACTTTGGGGAAACACTCAA+GGG 0.631484 4_2:-57015097 Msa0609660:CDS
ATGACCCAAGTGAAGCTGCA+AGG 0.632038 4_2:-57014972 Msa0609660:CDS
GTGTCCATGCTATTGCATCG+CGG 0.634519 4_2:+57015798 None:intergenic
ATGCCCGCGATGCAATAGCA+TGG 0.635421 4_2:-57015802 Msa0609660:CDS
CCAACCCTGCGGCTTTATCG+CGG 0.637960 4_2:-57014816 Msa0609660:CDS
GGGATGATCAGTGGAAGAAG+CGG 0.640321 4_2:+57017255 None:intergenic
GAGAGCTCTTAAATCCTCAG+TGG 0.645204 4_2:+57015974 None:intergenic
GCAGGGTTGGCATACATCAG+CGG 0.645757 4_2:+57014829 None:intergenic
GCTCAAGATAGCAGCTACTG+AGG 0.646149 4_2:+57014593 None:intergenic
CAAACTATGTAATCCATTAG+GGG 0.651674 4_2:+57015587 None:intergenic
TCAGATAGAAAAGGCCACAC+CGG 0.654081 4_2:-57015378 Msa0609660:CDS
CTAATTACAGCACAAGACGA+TGG 0.658149 4_2:-57016011 Msa0609660:CDS
AAAGCCAATTCTCTCAATGG+AGG 0.662101 4_2:-57014913 Msa0609660:CDS
TGCAATACAGCATGAAACTG+AGG 0.664237 4_2:-57017184 Msa0609660:intron
GCCATGGTGTTACAAAGCGG+AGG 0.666467 4_2:+57015528 None:intergenic
ATTGCAGAGGTGAATTCTGG+TGG 0.675104 4_2:+57017201 None:intergenic
TCCTCCGCTTTGTAACACCA+TGG 0.687107 4_2:-57015529 Msa0609660:CDS
AAGCTGCAAGGAGTTCATTG+TGG 0.697169 4_2:-57014960 Msa0609660:CDS
GGTAAAGGACATGAATCACA+CGG 0.721107 4_2:-57014863 Msa0609660:CDS
ATACAACCCCAATATGCAGG+TGG 0.724114 4_2:+57015201 None:intergenic
GAAGCCATGGTGTTACAAAG+CGG 0.731105 4_2:+57015525 None:intergenic
GAGACCGCGATAAAGCCGCA+GGG 0.731318 4_2:+57014812 None:intergenic
GCGGTCTCGTACCTTCCATG+AGG 0.732623 4_2:-57014797 Msa0609660:CDS
ACACATGGTATATTCCATGG+AGG 0.757531 4_2:+57015174 None:intergenic
GCTGATGTATGCCAACCCTG+CGG 0.770728 4_2:-57014827 Msa0609660:CDS
AGCCTCGCAACAAACCACTG+AGG 0.809905 4_2:-57015988 Msa0609660:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTGTATAAAAAAAAAAAG+GGG + chr4_2:57014445-57014464 None:intergenic 10.0%
!! ATTTATATAAAAATTGTTCA+TGG - chr4_2:57015356-57015375 Msa0609660:CDS 10.0%
!!! CAATTATCAATTTTTTGTTT+TGG - chr4_2:57015107-57015126 Msa0609660:CDS 15.0%
!! TATATAAAAATAGCAGAAAA+GGG + chr4_2:57015594-57015613 None:intergenic 15.0%
!! TTATATAAAAATAGCAGAAA+AGG + chr4_2:57015595-57015614 None:intergenic 15.0%
!!! TTAATCATATTCATTGTTTT+AGG - chr4_2:57015842-57015861 Msa0609660:CDS 15.0%
!! ATTAACTGCTTAAGAAAAAT+GGG + chr4_2:57014822-57014841 None:intergenic 20.0%
!! AATTAACTGCTTAAGAAAAA+TGG + chr4_2:57014823-57014842 None:intergenic 20.0%
!! TTAAGCAGTTAATTGTTAAT+GGG - chr4_2:57014829-57014848 Msa0609660:CDS 20.0%
!!! TTGAATCTTTTATTAAGTTG+TGG - chr4_2:57014965-57014984 Msa0609660:CDS 20.0%
!! TAAAACTAATCTATGGTATT+TGG + chr4_2:57015405-57015424 None:intergenic 20.0%
!!! AATACCATAGATTAGTTTTA+TGG - chr4_2:57015405-57015424 Msa0609660:CDS 20.0%
!!! TTTGTTTTTTACTTCTATTG+AGG - chr4_2:57015758-57015777 Msa0609660:CDS 20.0%
!! ATTGTTGAAGTAACAAAATT+TGG + chr4_2:57016120-57016139 None:intergenic 20.0%
!! TGATCTTTATTATTCTCAAA+AGG - chr4_2:57017213-57017232 Msa0609660:CDS 20.0%
!!! AGATTTGATAATATGTTGTT+GGG - chr4_2:57017352-57017371 Msa0609660:five_prime_UTR 20.0%
! TGAGTGAAAATATTATGAGA+TGG + chr4_2:57014526-57014545 None:intergenic 25.0%
! CTTAAGCAGTTAATTGTTAA+TGG - chr4_2:57014828-57014847 Msa0609660:CDS 25.0%
!! TTTTGATGTCAATTGAATTG+AGG - chr4_2:57014869-57014888 Msa0609660:CDS 25.0%
! AAATTGGATCGAATTTATTC+TGG - chr4_2:57015018-57015037 Msa0609660:CDS 25.0%
! TAACCAAAAAACTTCAATCA+AGG + chr4_2:57015206-57015225 None:intergenic 25.0%
!! CAAAATAGTTTAGTTCAAGT+CGG + chr4_2:57015238-57015257 None:intergenic 25.0%
!!! TTTATGGACTGTTAGTTTTT+AGG - chr4_2:57015421-57015440 Msa0609660:CDS 25.0%
!!! CTGCATATAATTTTAGTATG+AGG + chr4_2:57015630-57015649 None:intergenic 25.0%
! AGATTTCAATAACGAATTCT+CGG + chr4_2:57015997-57016016 None:intergenic 25.0%
!!! TTTCTTCAGAGTTTTTTCTT+CGG + chr4_2:57016054-57016073 None:intergenic 25.0%
! ACATTTCTTACAAAAATGAC+GGG + chr4_2:57016159-57016178 None:intergenic 25.0%
! GACATTTCTTACAAAAATGA+CGG + chr4_2:57016160-57016179 None:intergenic 25.0%
!! TAGATAAGTTTTTGTTCTTG+CGG + chr4_2:57016238-57016257 None:intergenic 25.0%
! TCAAATTCATCAGATAGAAA+AGG - chr4_2:57016506-57016525 Msa0609660:intron 25.0%
! TTTCAATGCAATTCACAATT+AGG + chr4_2:57017125-57017144 None:intergenic 25.0%
!! CAGATTTGATAATATGTTGT+TGG - chr4_2:57017351-57017370 Msa0609660:five_prime_UTR 25.0%
!!! ACAATAATGTTAGTCTTCAT+TGG - chr4_2:57017441-57017460 Msa0609660:five_prime_UTR 25.0%
! AAAAAAGGTAACAAAACAAC+AGG + chr4_2:57017470-57017489 None:intergenic 25.0%
GGGGATATTATATAGTTTGA+TGG + chr4_2:57014426-57014445 None:intergenic 30.0%
TCAATCATATGAAACCTTCA+TGG + chr4_2:57014741-57014760 None:intergenic 30.0%
GAAAAAACCAAAGCAAAACA+AGG + chr4_2:57015088-57015107 None:intergenic 30.0%
!!! TTGCCTTGATTGAAGTTTTT+TGG - chr4_2:57015200-57015219 Msa0609660:CDS 30.0%
! CGACTTGAACTAAACTATTT+TGG - chr4_2:57015236-57015255 Msa0609660:CDS 30.0%
!!! TGTTATTTTGACACAGAACA+AGG + chr4_2:57015289-57015308 None:intergenic 30.0%
AATTATGTTGTGATGTCACA+AGG + chr4_2:57015336-57015355 None:intergenic 30.0%
ATTGTTCATGGACATATTAC+TGG - chr4_2:57015368-57015387 Msa0609660:CDS 30.0%
CAGTCCATAAAACTAATCTA+TGG + chr4_2:57015412-57015431 None:intergenic 30.0%
! AATTTTAAGCGCAAAGTGAA+AGG + chr4_2:57015497-57015516 None:intergenic 30.0%
TCTATGAGAATCCATATATG+AGG - chr4_2:57015517-57015536 Msa0609660:CDS 30.0%
GCATACGTGTATAAAAACAT+TGG + chr4_2:57015689-57015708 None:intergenic 30.0%
GCATACGTGTATAAAAACAT+TGG + chr4_2:57015689-57015708 None:intergenic 30.0%
!! TGTTTTTATACACGTATGCT+CGG - chr4_2:57015717-57015736 Msa0609660:CDS 30.0%
AACTTACTGTGTACACAAAA+GGG + chr4_2:57015809-57015828 None:intergenic 30.0%
TAACTTACTGTGTACACAAA+AGG + chr4_2:57015810-57015829 None:intergenic 30.0%
! GTAATTAGTTCTTTCCTTGA+TGG + chr4_2:57015870-57015889 None:intergenic 30.0%
CATTTCTTACAAAAATGACG+GGG + chr4_2:57016158-57016177 None:intergenic 30.0%
GTAAGAAATGTCAAAGATAC+TGG - chr4_2:57016168-57016187 Msa0609660:intron 30.0%
CAAACTATGTAATCCATTAG+GGG + chr4_2:57016309-57016328 None:intergenic 30.0%
CCAAACTATGTAATCCATTA+GGG + chr4_2:57016310-57016329 None:intergenic 30.0%
!! CCTAATGGATTACATAGTTT+GGG - chr4_2:57016308-57016327 Msa0609660:intron 30.0%
ACTCAAAATTGTGTGTCAAA+TGG - chr4_2:57016413-57016432 Msa0609660:intron 30.0%
! TAGAATGCTAATGGATAGTT+TGG + chr4_2:57016659-57016678 None:intergenic 30.0%
!! TCATTTTCAGGCTTTTCTTT+TGG + chr4_2:57016851-57016870 None:intergenic 30.0%
CATCAAATCTAAGTGTCTTT+GGG + chr4_2:57016904-57016923 None:intergenic 30.0%
TCATCAAATCTAAGTGTCTT+TGG + chr4_2:57016905-57016924 None:intergenic 30.0%
AGGAGAGAATCTTCAAAAAA+AGG + chr4_2:57017485-57017504 None:intergenic 30.0%
!!! TGTGTGTGACTCAGTTTTTT+AGG + chr4_2:57014555-57014574 None:intergenic 35.0%
! TTTTCCTACAGAAATGTCAG+AGG - chr4_2:57014577-57014596 Msa0609660:three_prime_UTR 35.0%
TTTACCTCTGACATTTCTGT+AGG + chr4_2:57014584-57014603 None:intergenic 35.0%
ACTGCTTAAGAAAAATGGGT+TGG + chr4_2:57014818-57014837 None:intergenic 35.0%
GGAATTGCTGAAATTCTCAA+TGG - chr4_2:57014890-57014909 Msa0609660:CDS 35.0%
TGTGGCTCAAATTCATTATG+TGG - chr4_2:57014983-57015002 Msa0609660:CDS 35.0%
TTCGATCCAATTTCACTGAT+GGG + chr4_2:57015011-57015030 None:intergenic 35.0%
ATTCGATCCAATTTCACTGA+TGG + chr4_2:57015012-57015031 None:intergenic 35.0%
!!! TGGTTTACCTTGTTTTGCTT+TGG - chr4_2:57015078-57015097 Msa0609660:CDS 35.0%
ACAGAACAAGGACATGAATA+TGG + chr4_2:57015277-57015296 None:intergenic 35.0%
TATTGTAGGAGACAAGAGTA+AGG + chr4_2:57015447-57015466 None:intergenic 35.0%
!!! CATTGTTTTAGGAACCATCA+AGG - chr4_2:57015853-57015872 Msa0609660:CDS 35.0%
ACAATGAGAAATGTTCCTGT+TGG - chr4_2:57016203-57016222 Msa0609660:intron 35.0%
! TCGACATTTGATAATTCCAG+AGG - chr4_2:57016268-57016287 Msa0609660:intron 35.0%
! CGACATTTGATAATTCCAGA+GGG - chr4_2:57016269-57016288 Msa0609660:intron 35.0%
GCTAAACTTAATTCCCCTAA+TGG - chr4_2:57016293-57016312 Msa0609660:intron 35.0%
!! CCCTAATGGATTACATAGTT+TGG - chr4_2:57016307-57016326 Msa0609660:intron 35.0%
CCCAAACTATGTAATCCATT+AGG + chr4_2:57016311-57016330 None:intergenic 35.0%
GGATTACATAGTTTGGGAAA+TGG - chr4_2:57016314-57016333 Msa0609660:intron 35.0%
ATCATGCAAGTTCGTATAGT+AGG - chr4_2:57016441-57016460 Msa0609660:intron 35.0%
TCATGCAAGTTCGTATAGTA+GGG - chr4_2:57016442-57016461 Msa0609660:intron 35.0%
AGGGAAAATGATCACTCAAT+TGG - chr4_2:57016461-57016480 Msa0609660:intron 35.0%
AATGATCACTCAATTGGAGT+TGG - chr4_2:57016467-57016486 Msa0609660:intron 35.0%
!! ACAAATGGGTTGGTAGATAA+AGG - chr4_2:57016539-57016558 Msa0609660:intron 35.0%
TTGGTAGATAAAGGAGTTGA+AGG - chr4_2:57016548-57016567 Msa0609660:intron 35.0%
TTCCATGGATAAGGCAAAAA+GGG + chr4_2:57016605-57016624 None:intergenic 35.0%
ATTCCATGGATAAGGCAAAA+AGG + chr4_2:57016606-57016625 None:intergenic 35.0%
ATTGCAGAATTCCATGGATA+AGG + chr4_2:57016614-57016633 None:intergenic 35.0%
! AATGCTAATGGATAGTTTGG+TGG + chr4_2:57016656-57016675 None:intergenic 35.0%
ACTGGAGTATAGAATGCTAA+TGG + chr4_2:57016668-57016687 None:intergenic 35.0%
! TTCTTTTGGTGAATTGACTG+AGG + chr4_2:57016837-57016856 None:intergenic 35.0%
!! GTGCTATTGTGTTCATTTTC+AGG + chr4_2:57016863-57016882 None:intergenic 35.0%
TTCACAATTAGGTTTCCTCA+TGG + chr4_2:57017114-57017133 None:intergenic 35.0%
AAGAACTTGTCAGCAGAATA+AGG + chr4_2:57017194-57017213 None:intergenic 35.0%
! TTCCAAAGAGCTTTATCGCA+GGG + chr4_2:57014610-57014629 None:intergenic 40.0%
AATTCTGGTGGAGAAGAAGA+GGG + chr4_2:57014683-57014702 None:intergenic 40.0%
TGTATTGCAGAGGTGAATTC+TGG + chr4_2:57014698-57014717 None:intergenic 40.0%
AGTTTCATGCTGTATTGCAG+AGG + chr4_2:57014708-57014727 None:intergenic 40.0%
TGCAATACAGCATGAAACTG+AGG - chr4_2:57014709-57014728 Msa0609660:three_prime_UTR 40.0%
TCCTACAATACTTATGCCTC+TGG - chr4_2:57015457-57015476 Msa0609660:CDS 40.0%
! ACCAGAGGCATAAGTATTGT+AGG + chr4_2:57015461-57015480 None:intergenic 40.0%
TTAAGCGCAAAGTGAAAGGT+TGG + chr4_2:57015493-57015512 None:intergenic 40.0%
TGACTCTGACTCCTCATATA+TGG + chr4_2:57015531-57015550 None:intergenic 40.0%
CTAATTACAGCACAAGACGA+TGG - chr4_2:57015882-57015901 Msa0609660:CDS 40.0%
!! GGGCATGGAATTATTTTGTC+GGG + chr4_2:57016077-57016096 None:intergenic 40.0%
!! CGGGCATGGAATTATTTTGT+CGG + chr4_2:57016078-57016097 None:intergenic 40.0%
GAATTAAGTTTAGCACCCTC+TGG + chr4_2:57016287-57016306 None:intergenic 40.0%
GATGTTCAATACAGAAGCCA+TGG + chr4_2:57016384-57016403 None:intergenic 40.0%
GAATATGTTGCAACTGAGGA+GGG + chr4_2:57016577-57016596 None:intergenic 40.0%
CTTGGAATATGTTGCAACTG+AGG + chr4_2:57016581-57016600 None:intergenic 40.0%
! TAGTTTGGTGGACAAAAGGT+AGG + chr4_2:57016644-57016663 None:intergenic 40.0%
! TGGATAGTTTGGTGGACAAA+AGG + chr4_2:57016648-57016667 None:intergenic 40.0%
AGTAACTCCACCTGCATATT+GGG - chr4_2:57016685-57016704 Msa0609660:intron 40.0%
ACACATGGTATATTCCATGG+AGG + chr4_2:57016722-57016741 None:intergenic 40.0%
GAGACACATGGTATATTCCA+TGG + chr4_2:57016725-57016744 None:intergenic 40.0%
AATATACCATGTGTCTCACC+AGG - chr4_2:57016728-57016747 Msa0609660:intron 40.0%
!! TGATTAACAGAAGCACTTCC+TGG + chr4_2:57016749-57016768 None:intergenic 40.0%
AGTTCATTGTGGTCAAAGCT+TGG - chr4_2:57016944-57016963 Msa0609660:intron 40.0%
GTTCATTGTGGTCAAAGCTT+GGG - chr4_2:57016945-57016964 Msa0609660:intron 40.0%
GACAAAGCCAATTCTCTCAA+TGG - chr4_2:57016977-57016996 Msa0609660:intron 40.0%
AAAGCCAATTCTCTCAATGG+AGG - chr4_2:57016980-57016999 Msa0609660:intron 40.0%
! GTTCAATGGATTTCAGTCCA+AGG - chr4_2:57017009-57017028 Msa0609660:intron 40.0%
TGGATTTCAGTCCAAGGTAA+AGG - chr4_2:57017015-57017034 Msa0609660:intron 40.0%
GTGATTCATGTCCTTTACCT+TGG + chr4_2:57017029-57017048 None:intergenic 40.0%
GGTAAAGGACATGAATCACA+CGG - chr4_2:57017030-57017049 Msa0609660:intron 40.0%
CAATTAGGTTTCCTCATGGA+AGG + chr4_2:57017110-57017129 None:intergenic 40.0%
AAAGTACAACACTGTTGTCC+TGG + chr4_2:57017270-57017289 None:intergenic 40.0%
AAGAGAGAGAGAGAGAAGGA+GGG + chr4_2:57014397-57014416 None:intergenic 45.0%
AAAGAGAGAGAGAGAGAAGG+AGG + chr4_2:57014398-57014417 None:intergenic 45.0%
CAGAAAGAGAGAGAGAGAGA+AGG + chr4_2:57014401-57014420 None:intergenic 45.0%
! CTTCCAAAGAGCTTTATCGC+AGG + chr4_2:57014611-57014630 None:intergenic 45.0%
CTGCGATAAAGCTCTTTGGA+AGG - chr4_2:57014609-57014628 Msa0609660:three_prime_UTR 45.0%
GAGATGAAGAGTCATTGGCA+GGG + chr4_2:57014661-57014680 None:intergenic 45.0%
AGAGGGAGATGAAGAGTCAT+TGG + chr4_2:57014666-57014685 None:intergenic 45.0%
GAATTCTGGTGGAGAAGAAG+AGG + chr4_2:57014684-57014703 None:intergenic 45.0%
ATTGCAGAGGTGAATTCTGG+TGG + chr4_2:57014695-57014714 None:intergenic 45.0%
GTGGTTCCCATCAGTGAAAT+TGG - chr4_2:57015002-57015021 Msa0609660:CDS 45.0%
GAGAGCTCTTAAATCCTCAG+TGG + chr4_2:57015922-57015941 None:intergenic 45.0%
GTTTCAGCTGATGGAGTCTT+AGG + chr4_2:57015975-57015994 None:intergenic 45.0%
GAATTCTCGGTTTCAGCTGA+TGG + chr4_2:57015984-57016003 None:intergenic 45.0%
! TCTTACAAAAATGACGGGGC+TGG + chr4_2:57016154-57016173 None:intergenic 45.0%
TGTCAAAGATACTGGACTGC+AGG - chr4_2:57016176-57016195 Msa0609660:intron 45.0%
AGAAATGTTCCTGTTGGAGC+TGG - chr4_2:57016209-57016228 Msa0609660:intron 45.0%
!! GAAGCCATGGTGTTACAAAG+CGG + chr4_2:57016371-57016390 None:intergenic 45.0%
TCAGATAGAAAAGGCCACAC+CGG - chr4_2:57016515-57016534 Msa0609660:intron 45.0%
ATCTACCAACCCATTTGTGC+CGG + chr4_2:57016537-57016556 None:intergenic 45.0%
GGAATATGTTGCAACTGAGG+AGG + chr4_2:57016578-57016597 None:intergenic 45.0%
GGATAAGGCAAAAAGGGACT+TGG + chr4_2:57016599-57016618 None:intergenic 45.0%
! GTCCCTTTTTGCCTTATCCA+TGG - chr4_2:57016600-57016619 Msa0609660:intron 45.0%
! TTTGGTGGACAAAAGGTAGG+AGG + chr4_2:57016641-57016660 None:intergenic 45.0%
CAATATGCAGGTGGAGTTAC+TGG + chr4_2:57016686-57016705 None:intergenic 45.0%
CAGTAACTCCACCTGCATAT+TGG - chr4_2:57016684-57016703 Msa0609660:intron 45.0%
GTAACTCCACCTGCATATTG+GGG - chr4_2:57016686-57016705 Msa0609660:intron 45.0%
ATACAACCCCAATATGCAGG+TGG + chr4_2:57016695-57016714 None:intergenic 45.0%
CATGGTATATTCCATGGAGG+TGG + chr4_2:57016719-57016738 None:intergenic 45.0%
TTGAGTGTTTCCCCAAAGTC+GGG + chr4_2:57016796-57016815 None:intergenic 45.0%
CTTGAGTGTTTCCCCAAAGT+CGG + chr4_2:57016797-57016816 None:intergenic 45.0%
! GACTTTGGGGAAACACTCAA+GGG - chr4_2:57016796-57016815 Msa0609660:intron 45.0%
AACTCCTTGCAGCTTCACTT+GGG + chr4_2:57016928-57016947 None:intergenic 45.0%
AAGCTGCAAGGAGTTCATTG+TGG - chr4_2:57016933-57016952 Msa0609660:intron 45.0%
! AATGGAGGAGGCATGTTCAA+TGG - chr4_2:57016995-57017014 Msa0609660:intron 45.0%
AAACACCTGTTCAGTTAGGC+AGG + chr4_2:57017325-57017344 None:intergenic 45.0%
GCAGAAACACCTGTTCAGTT+AGG + chr4_2:57017329-57017348 None:intergenic 45.0%
!! AATTTGTATAAAAAAAAAAA+GGG + chr4_2:57014446-57014465 None:intergenic 5.0%
!!! TAATTTGTATAAAAAAAAAA+AGG + chr4_2:57014447-57014466 None:intergenic 5.0%
!! ATAATATTTAATATATTATG+TGG - chr4_2:57015058-57015077 Msa0609660:CDS 5.0%
GACCCTGCGATAAAGCTCTT+TGG - chr4_2:57014605-57014624 Msa0609660:three_prime_UTR 50.0%
GGGATGATCAGTGGAAGAAG+CGG + chr4_2:57014641-57014660 None:intergenic 50.0%
TCATTGGCAGGGATGATCAG+TGG + chr4_2:57014650-57014669 None:intergenic 50.0%
GGAGATGAAGAGTCATTGGC+AGG + chr4_2:57014662-57014681 None:intergenic 50.0%
AACTGAGGTACGTGCCATGA+AGG - chr4_2:57014724-57014743 Msa0609660:three_prime_UTR 50.0%
!! GGTTGGTGAATTCACACCAG+AGG + chr4_2:57015476-57015495 None:intergenic 50.0%
!! ATCCTCAGTGGTTTGTTGCG+AGG + chr4_2:57015910-57015929 None:intergenic 50.0%
TCAGCTGATGGAGTCTTAGG+AGG + chr4_2:57015972-57015991 None:intergenic 50.0%
TGTCCATGCTATTGCATCGC+GGG + chr4_2:57016097-57016116 None:intergenic 50.0%
GTGTCCATGCTATTGCATCG+CGG + chr4_2:57016098-57016117 None:intergenic 50.0%
CAAAGATACTGGACTGCAGG+AGG - chr4_2:57016179-57016198 Msa0609660:intron 50.0%
TCCTCCGCTTTGTAACACCA+TGG - chr4_2:57016364-57016383 Msa0609660:intron 50.0%
!! GGAGGCATTGCAGAATTCCA+TGG + chr4_2:57016620-57016639 None:intergenic 50.0%
GGCATACAACCCCAATATGC+AGG + chr4_2:57016698-57016717 None:intergenic 50.0%
TCTCAGTTCTGTCCCGACTT+TGG - chr4_2:57016781-57016800 Msa0609660:intron 50.0%
CTCAGTTCTGTCCCGACTTT+GGG - chr4_2:57016782-57016801 Msa0609660:intron 50.0%
TCAGTTCTGTCCCGACTTTG+GGG - chr4_2:57016783-57016802 Msa0609660:intron 50.0%
! CGACTTTGGGGAAACACTCA+AGG - chr4_2:57016795-57016814 Msa0609660:intron 50.0%
TTGGGGAAACACTCAAGGGA+TGG - chr4_2:57016800-57016819 Msa0609660:intron 50.0%
ATGACCCAAGTGAAGCTGCA+AGG - chr4_2:57016921-57016940 Msa0609660:intron 50.0%
GAACTCCTTGCAGCTTCACT+TGG + chr4_2:57016929-57016948 None:intergenic 50.0%
GCCAATTCTCTCAATGGAGG+AGG - chr4_2:57016983-57017002 Msa0609660:intron 50.0%
GCCTCCTCCATTGAGAGAAT+TGG + chr4_2:57016987-57017006 None:intergenic 50.0%
CTGGACTCTTGCATGGAGTT+TGG + chr4_2:57017251-57017270 None:intergenic 50.0%
CAAACTCCATGCAAGAGTCC+AGG - chr4_2:57017249-57017268 Msa0609660:CDS 50.0%
TGTTGTCCTGGACTCTTGCA+TGG + chr4_2:57017258-57017277 None:intergenic 50.0%
GCTCAAGATAGCAGCTACTG+AGG + chr4_2:57017303-57017322 None:intergenic 50.0%
TTGAGCCTGCCTAACTGAAC+AGG - chr4_2:57017317-57017336 Msa0609660:exon 50.0%
AGCCTCGCAACAAACCACTG+AGG - chr4_2:57015905-57015924 Msa0609660:CDS 55.0%
ATGCTATTGCATCGCGGGCA+TGG + chr4_2:57016092-57016111 None:intergenic 55.0%
ATGCCCGCGATGCAATAGCA+TGG - chr4_2:57016091-57016110 Msa0609660:intron 55.0%
!! GCCATGGTGTTACAAAGCGG+AGG + chr4_2:57016368-57016387 None:intergenic 55.0%
AAAGGCCACACCGGCACAAA+TGG - chr4_2:57016524-57016543 Msa0609660:intron 55.0%
AAGGCCACACCGGCACAAAT+GGG - chr4_2:57016525-57016544 Msa0609660:intron 55.0%
GGTGGACAAAAGGTAGGAGG+AGG + chr4_2:57016638-57016657 None:intergenic 55.0%
GCACTTCCTGGTGAGACACA+TGG + chr4_2:57016737-57016756 None:intergenic 55.0%
! GCAGGGTTGGCATACATCAG+CGG + chr4_2:57017067-57017086 None:intergenic 55.0%
GCTGATGTATGCCAACCCTG+CGG - chr4_2:57017066-57017085 Msa0609660:intron 55.0%
! CCAACCCATTTGTGCCGGTG+TGG + chr4_2:57016532-57016551 None:intergenic 60.0%
CCACACCGGCACAAATGGGT+TGG - chr4_2:57016529-57016548 Msa0609660:intron 60.0%
GGGGTTGTATGCCACCTCCA+TGG - chr4_2:57016705-57016724 Msa0609660:intron 60.0%
! CCAACCCTGCGGCTTTATCG+CGG - chr4_2:57017077-57017096 Msa0609660:intron 60.0%
GAGACCGCGATAAAGCCGCA+GGG + chr4_2:57017084-57017103 None:intergenic 60.0%
GCGGTCTCGTACCTTCCATG+AGG - chr4_2:57017096-57017115 Msa0609660:intron 60.0%
TTGCGGCGACCAGCTCCAAC+AGG + chr4_2:57016221-57016240 None:intergenic 65.0%
CCGCGATAAAGCCGCAGGGT+TGG + chr4_2:57017080-57017099 None:intergenic 65.0%
CGAGACCGCGATAAAGCCGC+AGG + chr4_2:57017085-57017104 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 57014393 57017522 57014393 ID=Msa0609660;Name=Msa0609660
chr4_2 mRNA 57014393 57017522 57014393 ID=Msa0609660-mRNA-1;Parent=Msa0609660;Name=Msa0609660-mRNA-1;_AED=0.00;_eAED=0.00;_QI=197|1|1|1|1|1|2|396|467
chr4_2 exon 57014393 57016051 57014393 ID=Msa0609660-mRNA-1:exon:14828;Parent=Msa0609660-mRNA-1
chr4_2 exon 57017185 57017522 57017185 ID=Msa0609660-mRNA-1:exon:14827;Parent=Msa0609660-mRNA-1
chr4_2 five_prime_UTR 57017326 57017522 57017326 ID=Msa0609660-mRNA-1:five_prime_utr;Parent=Msa0609660-mRNA-1
chr4_2 CDS 57017185 57017325 57017185 ID=Msa0609660-mRNA-1:cds;Parent=Msa0609660-mRNA-1
chr4_2 CDS 57014789 57016051 57014789 ID=Msa0609660-mRNA-1:cds;Parent=Msa0609660-mRNA-1
chr4_2 three_prime_UTR 57014393 57014788 57014393 ID=Msa0609660-mRNA-1:three_prime_utr;Parent=Msa0609660-mRNA-1
Gene Sequence

>Msa0609660

ATGTCAGAGGTAAAAGACCCTGCGATAAAGCTCTTTGGAAGGACAATTCCGCTTCTTCCACTGATCATCCCTGCCAATGACTCTTCATCTCCCTCTTCTTCTCCACCAGAATTCACCTCTGCAATACAGCATGAAACTGAGGAACCATCAAGGAAAGAACTAATTACAGCACAAGACGATGGAGCCTCGCAACAAACCACTGAGGATTTAAGAGCTCTCACAACATCTTCAGTTGTACTTGAGAATCCTCCTAAGACTCCATCAGCTGAAACCGAGAATTCGTTATTGAAATCTTCTACAAATGTAGAACAAAGCGAGACTAGTGTTTCCGAAGAAAAAACTCTGAAGAAACCCGACAAAATAATTCCATGCCCGCGATGCAATAGCATGGACACCAAATTTTGTTACTTCAACAATTACAATGTCAACCAGCCCCGTCATTTTTGTAAGAAATGTCAAAGATACTGGACTGCAGGAGGAACAATGAGAAATGTTCCTGTTGGAGCTGGTCGCCGCAAGAACAAAAACTTATCTAGCTCGCATTATCGACATTTGATAATTCCAGAGGGTGCTAAACTTAATTCCCCTAATGGATTACATAGTTTGGGAAATGGAGCAGCTGTTTTGACGTTTGATTCAAATCCTCCGCTTTGTAACACCATGGCTTCTGTATTGAACATCGCTGAAAGAACTCAAAATTGTGTGTCAAATGGTTTTCATCATGCAAGTTCGTATAGTAGGGAAAATGATCACTCAATTGGAGTTGGAGTATCAGTTACAACATCAAATTCATCAGATAGAAAAGGCCACACCGGCACAAATGGGTTGGTAGATAAAGGAGTTGAAGGTTTCCCTCCTCAGTTGCAACATATTCCAAGTCCCTTTTTGCCTTATCCATGGAATTCTGCAATGCCTCCTCCTACCTTTTGTCCACCAAACTATCCATTAGCATTCTATACTCCAGTAACTCCACCTGCATATTGGGGTTGTATGCCACCTCCATGGAATATACCATGTGTCTCACCAGGAAGTGCTTCTGTTAATCAAAGTGACTCTGCTCTCAGTTCTGTCCCGACTTTGGGGAAACACTCAAGGGATGGAAACATCATTACCTCAGTCAATTCACCAAAAGAAAAGCCTGAAAATGAACACAATAGCACTGAAAGCAGCGTTTTGATCCCAAAGACACTTAGATTTGATGACCCAAGTGAAGCTGCAAGGAGTTCATTGTGGTCAAAGCTTGGGATCAATAATGACAAAGCCAATTCTCTCAATGGAGGAGGCATGTTCAATGGATTTCAGTCCAAGGTAAAGGACATGAATCACACGGTTGAAACGTCACCGCTGATGTATGCCAACCCTGCGGCTTTATCGCGGTCTCGTACCTTCCATGAGGAAACCTAA
Protein sequence

>Msa0609660

MSEVKDPAIKLFGRTIPLLPLIIPANDSSSPSSSPPEFTSAIQHETEEPSRKELITAQDDGASQQTTEDLRALTTSSVVLENPPKTPSAETENSLLKSSTNVEQSETSVSEEKTLKKPDKIIPCPRCNSMDTKFCYFNNYNVNQPRHFCKKCQRYWTAGGTMRNVPVGAGRRKNKNLSSSHYRHLIIPEGAKLNSPNGLHSLGNGAAVLTFDSNPPLCNTMASVLNIAERTQNCVSNGFHHASSYSRENDHSIGVGVSVTTSNSSDRKGHTGTNGLVDKGVEGFPPQLQHIPSPFLPYPWNSAMPPPTFCPPNYPLAFYTPVTPPAYWGCMPPPWNIPCVSPGSASVNQSDSALSSVPTLGKHSRDGNIITSVNSPKEKPENEHNSTESSVLIPKTLRFDDPSEAARSSLWSKLGINNDKANSLNGGGMFNGFQSKVKDMNHTVETSPLMYANPAALSRSRTFHEET*