Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0666210 | XP_013442539.1 | 93.168 | 161 | 11 | 0 | 1 | 161 | 45 | 205 | 1.50e-107 | 315 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0666210 | A0A072TG65 | 93.168 | 161 | 11 | 0 | 1 | 161 | 45 | 205 | 7.18e-108 | 315 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0005050 | Msa0666210 | 0.805270 | 1.447938e-49 | -8.615850e-47 |
| Msa0048200 | Msa0666210 | 0.968414 | 1.210902e-128 | -8.615850e-47 |
| Msa0053110 | Msa0666210 | 0.806668 | 7.356412e-50 | -8.615850e-47 |
| Msa0098440 | Msa0666210 | 0.803612 | 3.207968e-49 | -8.615850e-47 |
| Msa0666210 | Msa0680150 | 0.814304 | 1.649210e-51 | -8.615850e-47 |
| Msa0666210 | Msa0747780 | 0.808118 | 3.623910e-50 | -8.615850e-47 |
| Msa0666210 | Msa0754030 | 0.802217 | 6.231048e-49 | -8.615850e-47 |
| Msa0666210 | Msa0807110 | 0.810521 | 1.106236e-50 | -8.615850e-47 |
| Msa0666210 | Msa0833040 | 0.812979 | 3.227437e-51 | -8.615850e-47 |
| Msa0666210 | Msa0867350 | 0.810953 | 8.917536e-51 | -8.615850e-47 |
| Msa0666210 | Msa0872310 | 0.816651 | 4.954141e-52 | -8.615850e-47 |
| Msa0666210 | Msa0873190 | 0.812004 | 5.272360e-51 | -8.615850e-47 |
| Msa0666210 | Msa0898730 | 0.817728 | 2.836846e-52 | -8.615850e-47 |
| Msa0666210 | Msa0905550 | 0.822566 | 2.211938e-53 | -8.615850e-47 |
| Msa0666210 | Msa0937550 | 0.828640 | 8.036768e-55 | -8.615850e-47 |
| Msa0666210 | Msa0999300 | 0.806100 | 9.693334e-50 | -8.615850e-47 |
| Msa0666210 | Msa1037900 | 0.824156 | 9.400056e-54 | -8.615850e-47 |
| Msa0666210 | Msa1041530 | 0.840102 | 1.064188e-57 | -8.615850e-47 |
| Msa0666210 | Msa1055260 | 0.801245 | 9.863049e-49 | -8.615850e-47 |
| Msa0666210 | Msa1055280 | 0.800567 | 1.356577e-48 | -8.615850e-47 |
| Msa0666210 | Msa1081570 | 0.846125 | 2.643775e-59 | -8.615850e-47 |
| Msa0666210 | Msa1123880 | 0.806714 | 7.191822e-50 | -8.615850e-47 |
| Msa0666210 | Msa1127140 | 0.857222 | 1.890415e-62 | -8.615850e-47 |
| Msa0666210 | Msa1129960 | 0.841250 | 5.326054e-58 | -8.615850e-47 |
| Msa0666210 | Msa1129970 | 0.817975 | 2.494792e-52 | -8.615850e-47 |
| Msa0666210 | Msa1130010 | 0.821630 | 3.645316e-53 | -8.615850e-47 |
| Msa0666210 | Msa1166400 | 0.857732 | 1.335697e-62 | -8.615850e-47 |
| Msa0666210 | Msa1176160 | 0.829424 | 5.189200e-55 | -8.615850e-47 |
| Msa0666210 | Msa1307190 | 0.828371 | 9.328597e-55 | -8.615850e-47 |
| Msa0666210 | Msa1408870 | 0.811077 | 8.384278e-51 | -8.615850e-47 |
| Msa0666210 | Msa1420060 | 0.854724 | 1.017161e-61 | -8.615850e-47 |
| Msa0666210 | Msa1421550 | 0.826567 | 2.527152e-54 | -8.615850e-47 |
| Msa0134130 | Msa0666210 | 0.803640 | 3.165221e-49 | -8.615850e-47 |
| Msa0219190 | Msa0666210 | 0.830705 | 2.526058e-55 | -8.615850e-47 |
| Msa0378340 | Msa0666210 | 0.803880 | 2.822638e-49 | -8.615850e-47 |
| Msa0427690 | Msa0666210 | 0.803469 | 3.434237e-49 | -8.615850e-47 |
| Msa0436500 | Msa0666210 | 0.801853 | 7.402145e-49 | -8.615850e-47 |
| Msa0289890 | Msa0666210 | 0.807498 | 4.908693e-50 | -8.615850e-47 |
| Msa0298240 | Msa0666210 | 0.812347 | 4.438084e-51 | -8.615850e-47 |
| Msa0308830 | Msa0666210 | 0.803969 | 2.705161e-49 | -8.615850e-47 |
| Msa0518520 | Msa0666210 | 0.805198 | 1.498832e-49 | -8.615850e-47 |
| Msa0531630 | Msa0666210 | 0.829990 | 3.778185e-55 | -8.615850e-47 |
| Msa0537920 | Msa0666210 | 0.818451 | 1.947334e-52 | -8.615850e-47 |
| Msa0538060 | Msa0666210 | 0.806449 | 8.180174e-50 | -8.615850e-47 |
| Msa0538220 | Msa0666210 | 0.801231 | 9.928796e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0666210 | MtrunA17_Chr4g0004611 | 93.168 | 161 | 11 | 0 | 1 | 161 | 45 | 205 | 1.38e-111 | 315 |
| Msa0666210 | MtrunA17_Chr2g0331751 | 48.193 | 166 | 68 | 5 | 2 | 158 | 47 | 203 | 4.68e-40 | 134 |
| Msa0666210 | MtrunA17_Chr8g0390541 | 38.202 | 89 | 47 | 2 | 65 | 145 | 103 | 191 | 2.55e-15 | 70.1 |
| Msa0666210 | MtrunA17_Chr6g0485651 | 31.193 | 109 | 59 | 3 | 44 | 136 | 60 | 168 | 2.45e-13 | 64.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0666210 | AT1G15260.1 | 39.423 | 104 | 60 | 1 | 58 | 158 | 75 | 178 | 5.45e-18 | 77.0 |
| Msa0666210 | AT3G16070.1 | 46.377 | 69 | 36 | 1 | 67 | 135 | 83 | 150 | 2.24e-15 | 69.3 |
Find 25 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAATTCACAAAACATGAATA+AGG | 0.296971 | 4_4:-6022071 | Msa0666210:CDS |
| CTTGCAGTATCTTCCATTGC+AGG | 0.306986 | 4_4:+6022187 | None:intergenic |
| CCTTCATAAGTGCTTCTTTG+TGG | 0.310981 | 4_4:+6022130 | None:intergenic |
| GCTTCTTTGTGGACTTTGTT+TGG | 0.335982 | 4_4:+6022141 | None:intergenic |
| CATAGAACTCATTGTAGAAT+TGG | 0.346345 | 4_4:+6022263 | None:intergenic |
| AGCACAGAAGATGAAGAAAT+TGG | 0.353260 | 4_4:-6022035 | Msa0666210:CDS |
| GATGAAGAAATTGGATATGA+TGG | 0.381301 | 4_4:-6022026 | Msa0666210:CDS |
| ACGATCACAACTGAGGAAAA+AGG | 0.381662 | 4_4:-6022101 | Msa0666210:CDS |
| CAGAGGGCTGTGGAAGAGTC+AGG | 0.415158 | 4_4:+6021885 | None:intergenic |
| CTCAGCACAATGAAGATGTT+TGG | 0.428580 | 4_4:-6022223 | Msa0666210:CDS |
| TCCGGTTTATTTAGATATTG+TGG | 0.429248 | 4_4:-6021936 | Msa0666210:CDS |
| AACTGGACCAAGTCTTTCCT+TGG | 0.434890 | 4_4:+6021847 | None:intergenic |
| CTATTCTCGCCTTAAAAGTC+CGG | 0.438812 | 4_4:-6021954 | Msa0666210:CDS |
| TCCACAATATCTAAATAAAC+CGG | 0.450069 | 4_4:+6021935 | None:intergenic |
| AAATTGGATATGATGGAATC+AGG | 0.453910 | 4_4:-6022019 | Msa0666210:CDS |
| TTGAAGCTGGCAGAGGGCTG+TGG | 0.468785 | 4_4:+6021875 | None:intergenic |
| AATTCACAAAACATGAATAA+GGG | 0.481457 | 4_4:-6022070 | Msa0666210:CDS |
| TTTATTTAGATATTGTGGAC+AGG | 0.515044 | 4_4:-6021931 | Msa0666210:CDS |
| ACAATGAAGATGTTTGGTAA+AGG | 0.547940 | 4_4:-6022217 | Msa0666210:CDS |
| CCACAAAGAAGCACTTATGA+AGG | 0.551698 | 4_4:-6022130 | Msa0666210:CDS |
| AAAAGATCCAAGGAAAGACT+TGG | 0.563595 | 4_4:-6021854 | Msa0666210:CDS |
| ATGGAAGATACTGCAAGAAG+TGG | 0.578425 | 4_4:-6022181 | Msa0666210:CDS |
| TGCCAGCTTCAAAAGATCCA+AGG | 0.579056 | 4_4:-6021864 | Msa0666210:CDS |
| TAAAGGAAAAGAGCCTGCAA+TGG | 0.618557 | 4_4:-6022200 | Msa0666210:CDS |
| GTAGCGTACGATCACAACTG+AGG | 0.731543 | 4_4:-6022108 | Msa0666210:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTCACAAAACATGAATAA+GGG | - | chr4_4:6022051-6022070 | Msa0666210:CDS | 20.0% |
| ! | GAATTCACAAAACATGAATA+AGG | - | chr4_4:6022050-6022069 | Msa0666210:CDS | 25.0% |
| ! | TCCACAATATCTAAATAAAC+CGG | + | chr4_4:6022189-6022208 | None:intergenic | 25.0% |
| ! | TTTATTTAGATATTGTGGAC+AGG | - | chr4_4:6022190-6022209 | Msa0666210:CDS | 25.0% |
| !! | TATCTTTTATGTAACTGAAC+TGG | + | chr4_4:6022294-6022313 | None:intergenic | 25.0% |
| CATAGAACTCATTGTAGAAT+TGG | + | chr4_4:6021861-6021880 | None:intergenic | 30.0% | |
| ACAATGAAGATGTTTGGTAA+AGG | - | chr4_4:6021904-6021923 | Msa0666210:CDS | 30.0% | |
| GATGAAGAAATTGGATATGA+TGG | - | chr4_4:6022095-6022114 | Msa0666210:CDS | 30.0% | |
| ! | AAATTGGATATGATGGAATC+AGG | - | chr4_4:6022102-6022121 | Msa0666210:CDS | 30.0% |
| ! | CTAAATAAACCGGACTTTTA+AGG | + | chr4_4:6022179-6022198 | None:intergenic | 30.0% |
| TCCGGTTTATTTAGATATTG+TGG | - | chr4_4:6022185-6022204 | Msa0666210:CDS | 30.0% | |
| AGCACAGAAGATGAAGAAAT+TGG | - | chr4_4:6022086-6022105 | Msa0666210:CDS | 35.0% | |
| AAAAGATCCAAGGAAAGACT+TGG | - | chr4_4:6022267-6022286 | Msa0666210:CDS | 35.0% | |
| CTCAGCACAATGAAGATGTT+TGG | - | chr4_4:6021898-6021917 | Msa0666210:CDS | 40.0% | |
| TAAAGGAAAAGAGCCTGCAA+TGG | - | chr4_4:6021921-6021940 | Msa0666210:CDS | 40.0% | |
| ATGGAAGATACTGCAAGAAG+TGG | - | chr4_4:6021940-6021959 | Msa0666210:CDS | 40.0% | |
| GCTTCTTTGTGGACTTTGTT+TGG | + | chr4_4:6021983-6022002 | None:intergenic | 40.0% | |
| CCTTCATAAGTGCTTCTTTG+TGG | + | chr4_4:6021994-6022013 | None:intergenic | 40.0% | |
| !! | CCACAAAGAAGCACTTATGA+AGG | - | chr4_4:6021991-6022010 | Msa0666210:CDS | 40.0% |
| ACGATCACAACTGAGGAAAA+AGG | - | chr4_4:6022020-6022039 | Msa0666210:CDS | 40.0% | |
| ATCACAACTGAGGAAAAAGG+AGG | - | chr4_4:6022023-6022042 | Msa0666210:CDS | 40.0% | |
| CTATTCTCGCCTTAAAAGTC+CGG | - | chr4_4:6022167-6022186 | Msa0666210:CDS | 40.0% | |
| !!! | TTCCTTGGATCTTTTGAAGC+TGG | + | chr4_4:6022262-6022281 | None:intergenic | 40.0% |
| CTTGCAGTATCTTCCATTGC+AGG | + | chr4_4:6021937-6021956 | None:intergenic | 45.0% | |
| !!! | GATCTTTTGAAGCTGGCAGA+GGG | + | chr4_4:6022255-6022274 | None:intergenic | 45.0% |
| TGCCAGCTTCAAAAGATCCA+AGG | - | chr4_4:6022257-6022276 | Msa0666210:CDS | 45.0% | |
| AACTGGACCAAGTCTTTCCT+TGG | + | chr4_4:6022277-6022296 | None:intergenic | 45.0% | |
| GTAGCGTACGATCACAACTG+AGG | - | chr4_4:6022013-6022032 | Msa0666210:CDS | 50.0% | |
| !!! | GGATCTTTTGAAGCTGGCAG+AGG | + | chr4_4:6022256-6022275 | None:intergenic | 50.0% |
| CAGAGGGCTGTGGAAGAGTC+AGG | + | chr4_4:6022239-6022258 | None:intergenic | 60.0% | |
| TTGAAGCTGGCAGAGGGCTG+TGG | + | chr4_4:6022249-6022268 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_4 | gene | 6021829 | 6022314 | 6021829 | ID=Msa0666210;Name=Msa0666210 |
| chr4_4 | mRNA | 6021829 | 6022314 | 6021829 | ID=Msa0666210-mRNA-1;Parent=Msa0666210;Name=Msa0666210-mRNA-1;_AED=0.31;_eAED=0.31;_QI=0|-1|0|1|-1|1|1|0|161 |
| chr4_4 | exon | 6021829 | 6022314 | 6021829 | ID=Msa0666210-mRNA-1:exon:2002;Parent=Msa0666210-mRNA-1 |
| chr4_4 | CDS | 6021829 | 6022314 | 6021829 | ID=Msa0666210-mRNA-1:cds;Parent=Msa0666210-mRNA-1 |
| Gene Sequence |
| Protein sequence |
