AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0707770

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0707770 MtrunA17_Chr8g0390391 86.957 92 10 1 1 90 1 92 5.82e-48 149
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0707770 AT1G55205.1 46.429 84 38 1 14 90 8 91 6.45e-15 65.9
Msa0707770 AT1G55205.2 46.429 84 38 1 14 90 8 91 9.80e-15 65.5
Msa0707770 AT3G13674.1 51.648 91 37 3 4 90 2 89 4.35e-12 58.5

Find 57 sgRNAs with CRISPR-Local

Find 267 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACTAATAATGTGTTATTAA+AGG 0.206746 4_4:+81236164 Msa0707770:three_prime_UTR
GTCCATTGAATCTCTTGGTT+GGG 0.256157 4_4:+81233461 Msa0707770:CDS
AATAACTCTTGATAAATAAA+TGG 0.309560 4_4:-81235762 None:intergenic
CACGCGTCTCTCTCACTTCT+CGG 0.330178 4_4:+81233250 Msa0707770:CDS
AACATCGTCGTGTAAGGCTT+TGG 0.356385 4_4:+81233125 Msa0707770:CDS
ACTAATAATGTGTTATTAAA+GGG 0.381020 4_4:+81236165 Msa0707770:three_prime_UTR
ATTGAGGTTTGAGTTTGAAA+TGG 0.391472 4_4:-81233215 None:intergenic
TCCATTGAATCTCTTGGTTG+GGG 0.410909 4_4:+81233462 Msa0707770:CDS
AGCTTGTCCATTGAATCTCT+TGG 0.420866 4_4:+81233456 Msa0707770:CDS
TAAGTTATCCCAGAATCGTC+TGG 0.426313 4_4:-81236108 None:intergenic
TGTCCATTGAATCTCTTGGT+TGG 0.430095 4_4:+81233460 Msa0707770:CDS
GCAGATCAACACTGTCATAA+AGG 0.430723 4_4:-81235914 None:intergenic
CGTGGAACAGTTGCAGATTG+AGG 0.437039 4_4:-81233231 None:intergenic
ACATCGTCGTGTAAGGCTTT+GGG 0.447180 4_4:+81233126 Msa0707770:CDS
AAGCCTCTTCCTTCGATGCT+AGG 0.451190 4_4:-81233023 None:intergenic
CTGTGATCACCACAGCTAGA+AGG 0.451916 4_4:+81236226 Msa0707770:three_prime_UTR
ACCCCAACCAAGAGATTCAA+TGG 0.458951 4_4:-81233463 None:intergenic
CCTTGCTTAACACAGGTGAA+TGG 0.462019 4_4:+81236314 Msa0707770:three_prime_UTR
TGACGCGACCGCGCTATACA+AGG 0.462998 4_4:-81233418 None:intergenic
TTCCATGTGATGGCAGACTT+TGG 0.467885 4_4:+81235959 Msa0707770:exon
ACCGGTGGAGTTGTGTAGCT+TGG 0.471908 4_4:+81233383 Msa0707770:CDS
ATCACAGAAATGATAACAAA+TGG 0.473661 4_4:-81236210 None:intergenic
CTTGCTTAACACAGGTGAAT+GGG 0.474988 4_4:+81236315 Msa0707770:three_prime_UTR
GTAAAGGATCCTATGTCAAA+AGG 0.479341 4_4:-81236019 None:intergenic
AAGAAGAAGAAGAATTAGAA+TGG 0.485726 4_4:+81233101 Msa0707770:exon
GCGGGGTGACAGTGTCGCTT+AGG 0.489316 4_4:+81233056 Msa0707770:five_prime_UTR
AATGGCAACATCGTCGTGTA+AGG 0.489393 4_4:+81233119 Msa0707770:exon
GAATAATGATTGTTTAGTGT+TGG 0.492864 4_4:-81233187 None:intergenic
AACAAGAGCTTGTAATCGAC+AGG 0.496070 4_4:-81233157 None:intergenic
ATGGTTGTTGCTTAATGTAA+AGG 0.496684 4_4:+81236280 Msa0707770:three_prime_UTR
GGTTGGGGTTTAGTTCCTCA+AGG 0.501357 4_4:+81233477 Msa0707770:CDS
CAACTAGCATTTCCATGTGA+TGG 0.513606 4_4:+81235949 Msa0707770:CDS
TTCGTCAAAGATATTAGTAA+AGG 0.514411 4_4:-81236035 None:intergenic
AGAGGCTTTGAAAGAAAAGC+GGG 0.531946 4_4:+81233038 Msa0707770:five_prime_UTR
TCTTCATCCTTGCTTAACAC+AGG 0.548856 4_4:+81236307 Msa0707770:three_prime_UTR
GGTCCTAGCATCGAAGGAAG+AGG 0.562844 4_4:+81233020 Msa0707770:five_prime_UTR
AAGTTATCCCAGAATCGTCT+GGG 0.563192 4_4:-81236107 None:intergenic
GACGCGACCGCGCTATACAA+GGG 0.566258 4_4:-81233417 None:intergenic
CCATTCACCTGTGTTAAGCA+AGG 0.573401 4_4:-81236314 None:intergenic
ATTATCAAACTAGTGTGGAA+TGG 0.583202 4_4:+81236261 Msa0707770:three_prime_UTR
AGCCAAAGTCTGCCATCACA+TGG 0.603009 4_4:-81235961 None:intergenic
TTAGAAATACCTTCTAGCTG+TGG 0.604341 4_4:-81236235 None:intergenic
AAATCATTATCAAACTAGTG+TGG 0.606487 4_4:+81236256 Msa0707770:three_prime_UTR
TCAACACTGTCATAAAGGCA+TGG 0.609858 4_4:-81235909 None:intergenic
TTTGTTGATTAGGTTACCGG+TGG 0.609872 4_4:+81233368 Msa0707770:intron
CTTAACACAGGTGAATGGGA+AGG 0.619978 4_4:+81236319 Msa0707770:three_prime_UTR
TCCAAGCTACACAACTCCAC+CGG 0.640795 4_4:-81233384 None:intergenic
TCGTATGGTCCTAGCATCGA+AGG 0.641007 4_4:+81233014 None:intergenic
CGTCGTGTAAGGCTTTGGGT+AGG 0.641254 4_4:+81233130 Msa0707770:CDS
GATATGTCAACTGCACATGA+CGG 0.658720 4_4:-81236372 None:intergenic
GTGAATGGGAAGGAAATACA+TGG 0.662933 4_4:+81236329 Msa0707770:three_prime_UTR
AAGAGGCTTTGAAAGAAAAG+CGG 0.667610 4_4:+81233037 Msa0707770:five_prime_UTR
TTTATGACAGTGTTGATCTG+CGG 0.667904 4_4:+81235916 Msa0707770:CDS
CGAGAAGTGAGAGAGACGCG+TGG 0.689340 4_4:-81233249 None:intergenic
AATGCTGCCCTTGTATAGCG+CGG 0.709995 4_4:+81233410 Msa0707770:CDS
AGTTATCCCAGAATCGTCTG+GGG 0.718943 4_4:-81236106 None:intergenic
GAGGCTTTGAAAGAAAAGCG+GGG 0.758586 4_4:+81233039 Msa0707770:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATAATAAAATGAAAGATT+AGG - chr4_4:81233304-81233323 None:intergenic 10.0%
!!! GTTTTTTTTTTTTTTTGAAT+CGG - chr4_4:81235436-81235455 None:intergenic 10.0%
!! TTTGAAAATTTAAAAGCAAT+GGG - chr4_4:81234268-81234287 None:intergenic 15.0%
!! AATAACTCTTGATAAATAAA+TGG - chr4_4:81235765-81235784 None:intergenic 15.0%
!!! AACTAATAATGTGTTATTAA+AGG + chr4_4:81236164-81236183 Msa0707770:three_prime_UTR 15.0%
!!! ACTAATAATGTGTTATTAAA+GGG + chr4_4:81236165-81236184 Msa0707770:three_prime_UTR 15.0%
!! AATAAAATGAAAGATTAGGT+AGG - chr4_4:81233300-81233319 None:intergenic 20.0%
!!! TTTCTTCAATTTTGTTGATT+AGG + chr4_4:81233358-81233377 Msa0707770:intron 20.0%
!! TTATTTAAATGAAGACTGTT+TGG + chr4_4:81233631-81233650 Msa0707770:intron 20.0%
!! TATTTAAATGAAGACTGTTT+GGG + chr4_4:81233632-81233651 Msa0707770:intron 20.0%
!! TTTGAACTGAAATATTAGTA+AGG + chr4_4:81233915-81233934 Msa0707770:intron 20.0%
!!! CTTTGAAAATTTAAAAGCAA+TGG - chr4_4:81234269-81234288 None:intergenic 20.0%
!! AATAAAATAGGGATAAAGTA+AGG - chr4_4:81234329-81234348 None:intergenic 20.0%
!!! TTTATTTTGACAGTTTTCTT+TGG + chr4_4:81235201-81235220 Msa0707770:intron 20.0%
!!! TTATTTTGACAGTTTTCTTT+GGG + chr4_4:81235202-81235221 Msa0707770:intron 20.0%
!!! TTTACAGTGTTTGTTTTTTT+TGG + chr4_4:81235261-81235280 Msa0707770:intron 20.0%
!! AATCGGCAAATATTATTAAA+CGG - chr4_4:81235419-81235438 None:intergenic 20.0%
!!! TTCATTTTTTTGTAATCTTG+TGG + chr4_4:81235996-81236015 Msa0707770:three_prime_UTR 20.0%
! AAGAAGAAGAAGAATTAGAA+TGG + chr4_4:81233101-81233120 Msa0707770:exon 25.0%
! GAATAATGATTGTTTAGTGT+TGG - chr4_4:81233190-81233209 None:intergenic 25.0%
!!! AATTTTGTTGATTAGGTTAC+CGG + chr4_4:81233365-81233384 Msa0707770:intron 25.0%
! TAGCTTATATGAAAACAGTT+TGG + chr4_4:81233715-81233734 Msa0707770:intron 25.0%
!!! ATAAGTTATTTCTACAGCAA+AGG - chr4_4:81233752-81233771 None:intergenic 25.0%
!! TTAAGTTTTTCCATTGCTTT+GGG + chr4_4:81233890-81233909 Msa0707770:intron 25.0%
! AACTGAAATATTAGTAAGGA+TGG + chr4_4:81233919-81233938 Msa0707770:intron 25.0%
!! TTCAAGAACATGGATTAAAA+TGG + chr4_4:81233968-81233987 Msa0707770:intron 25.0%
!!! GATGGTTGATGTTTTATTTT+AGG + chr4_4:81234649-81234668 Msa0707770:intron 25.0%
!!! TATTTTGACAGTTTTCTTTG+GGG + chr4_4:81235203-81235222 Msa0707770:intron 25.0%
!!! TTTTTTTTGGAGTCTGTTTT+AGG + chr4_4:81235274-81235293 Msa0707770:intron 25.0%
!!! CATTTTTTAATATGCACTAC+TGG + chr4_4:81235476-81235495 Msa0707770:intron 25.0%
! TACTTCTTACAAGTGTTAAA+GGG - chr4_4:81235691-81235710 None:intergenic 25.0%
!!! GTAAGAAGTACAAAATTTTC+CGG + chr4_4:81235701-81235720 Msa0707770:intron 25.0%
!!! ATTCTTTTAAAGCACATAGT+TGG + chr4_4:81235782-81235801 Msa0707770:CDS 25.0%
! TACCAATTATAATAGCTGAA+TGG + chr4_4:81235846-81235865 Msa0707770:intron 25.0%
! TTCCATTCAGCTATTATAAT+TGG - chr4_4:81235851-81235870 None:intergenic 25.0%
!!! ACAATGCTTTTGTTTTTTAC+AGG + chr4_4:81235881-81235900 Msa0707770:intron 25.0%
! TTCGTCAAAGATATTAGTAA+AGG - chr4_4:81236038-81236057 None:intergenic 25.0%
!! TATTTTGCCTTTCCTATTTT+TGG + chr4_4:81236129-81236148 Msa0707770:three_prime_UTR 25.0%
! ATCACAGAAATGATAACAAA+TGG - chr4_4:81236213-81236232 None:intergenic 25.0%
! AAATCATTATCAAACTAGTG+TGG + chr4_4:81236256-81236275 Msa0707770:three_prime_UTR 25.0%
! ATTGAGGTTTGAGTTTGAAA+TGG - chr4_4:81233218-81233237 None:intergenic 30.0%
TATTATTCACAAGCTTGTCA+AGG - chr4_4:81233596-81233615 None:intergenic 30.0%
!! GTCATTTTCATATGGTATCA+TGG - chr4_4:81233692-81233711 None:intergenic 30.0%
!! AGCTACAAGTCATTTTCATA+TGG - chr4_4:81233700-81233719 None:intergenic 30.0%
TCAAATATTTACTTTCCCAG+TGG - chr4_4:81233826-81233845 None:intergenic 30.0%
! CTTAAGTTTTTCCATTGCTT+TGG + chr4_4:81233889-81233908 Msa0707770:intron 30.0%
!!! CGATAAGTTTTTCAAGAACA+TGG + chr4_4:81233958-81233977 Msa0707770:intron 30.0%
!! TGTGCCATTCATTTTATGTT+GGG + chr4_4:81234020-81234039 Msa0707770:intron 30.0%
CAAACCCAACATAAAATGAA+TGG - chr4_4:81234027-81234046 None:intergenic 30.0%
! TATTTGAGTTCCGATCATAT+GGG - chr4_4:81234085-81234104 None:intergenic 30.0%
! ATATTTGAGTTCCGATCATA+TGG - chr4_4:81234086-81234105 None:intergenic 30.0%
TGTACCTAAACAAATACACT+AGG + chr4_4:81234164-81234183 Msa0707770:intron 30.0%
TGTACTAAAGCCTGAAATAA+AGG + chr4_4:81234210-81234229 Msa0707770:intron 30.0%
GTACTAAAGCCTGAAATAAA+GGG + chr4_4:81234211-81234230 Msa0707770:intron 30.0%
! ATCTACTATCCCTTTATTTC+AGG - chr4_4:81234223-81234242 None:intergenic 30.0%
TAGGGATAAAGTAAGGTATA+TGG - chr4_4:81234322-81234341 None:intergenic 30.0%
! TCCCTATTTTATTGTGAGAT+AGG + chr4_4:81234336-81234355 Msa0707770:intron 30.0%
CCCTATCTCACAATAAAATA+GGG - chr4_4:81234340-81234359 None:intergenic 30.0%
! CCCTATTTTATTGTGAGATA+GGG + chr4_4:81234337-81234356 Msa0707770:intron 30.0%
! ATTTTATAGTGCAGCTATCA+GGG - chr4_4:81234399-81234418 None:intergenic 30.0%
!!! GGTTGATGTTTTATTTTAGG+AGG + chr4_4:81234652-81234671 Msa0707770:intron 30.0%
!!! TGATGTTTTATTTTAGGAGG+TGG + chr4_4:81234655-81234674 Msa0707770:intron 30.0%
!!! GATGTTTTATTTTAGGAGGT+GGG + chr4_4:81234656-81234675 Msa0707770:intron 30.0%
TAAACAACACACATAACCAA+AGG - chr4_4:81234825-81234844 None:intergenic 30.0%
!!! CATATTGTTTGCTGGTTTTT+CGG + chr4_4:81234872-81234891 Msa0707770:intron 30.0%
!! ATTTTGACAGTTTTCTTTGG+GGG + chr4_4:81235204-81235223 Msa0707770:intron 30.0%
!!! TTTTAGGTGTTGTTGTGTTT+TGG + chr4_4:81235290-81235309 Msa0707770:intron 30.0%
!!! TTTTGGTGGGTTTATTTTCA+TGG + chr4_4:81235307-81235326 Msa0707770:intron 30.0%
!!! TTTGGTGGGTTTATTTTCAT+GGG + chr4_4:81235308-81235327 Msa0707770:intron 30.0%
! TATTTTCATGGGTGTTTCAT+AGG + chr4_4:81235319-81235338 Msa0707770:intron 30.0%
TGAACGAAACAAGATACAAA+TGG - chr4_4:81235374-81235393 None:intergenic 30.0%
AATTGTGATAGAGAGAACAT+GGG - chr4_4:81235576-81235595 None:intergenic 30.0%
TAATTGTGATAGAGAGAACA+TGG - chr4_4:81235577-81235596 None:intergenic 30.0%
GTACTTCTTACAAGTGTTAA+AGG - chr4_4:81235692-81235711 None:intergenic 30.0%
!! TTACATAAAAGTACTCATGC+CGG - chr4_4:81235723-81235742 None:intergenic 30.0%
AACTCATCCAAAAATAGGAA+AGG - chr4_4:81236139-81236158 None:intergenic 30.0%
!!! TTCCTATTTTTGGATGAGTT+TGG + chr4_4:81236139-81236158 Msa0707770:three_prime_UTR 30.0%
TACCAAACTCATCCAAAAAT+AGG - chr4_4:81236144-81236163 None:intergenic 30.0%
ATTATCAAACTAGTGTGGAA+TGG + chr4_4:81236261-81236280 Msa0707770:three_prime_UTR 30.0%
! ATGGTTGTTGCTTAATGTAA+AGG + chr4_4:81236280-81236299 Msa0707770:three_prime_UTR 30.0%
!! AAGAGGCTTTGAAAGAAAAG+CGG + chr4_4:81233037-81233056 Msa0707770:five_prime_UTR 35.0%
CTTGAAAAACTTATCGAAGC+AGG - chr4_4:81233954-81233973 None:intergenic 35.0%
!! GTGTGCCATTCATTTTATGT+TGG + chr4_4:81234019-81234038 Msa0707770:intron 35.0%
TTTATGTTGGGTTTGACGTA+AGG + chr4_4:81234032-81234051 Msa0707770:intron 35.0%
GCCCTATCTCACAATAAAAT+AGG - chr4_4:81234341-81234360 None:intergenic 35.0%
TGTCACTAACTATATGAGGT+TGG - chr4_4:81234363-81234382 None:intergenic 35.0%
AACCTCATATAGTTAGTGAC+AGG + chr4_4:81234362-81234381 Msa0707770:intron 35.0%
ACCTCATATAGTTAGTGACA+GGG + chr4_4:81234363-81234382 Msa0707770:intron 35.0%
ATATAGTTAGTGACAGGGTT+GGG + chr4_4:81234368-81234387 Msa0707770:intron 35.0%
! CATTTTATAGTGCAGCTATC+AGG - chr4_4:81234400-81234419 None:intergenic 35.0%
!!! GTTTAGGTGTTTTTGGAGTT+TGG + chr4_4:81234768-81234787 Msa0707770:intron 35.0%
AGGAAAAGGCTTAACACAAT+GGG - chr4_4:81234805-81234824 None:intergenic 35.0%
AAGGAAAAGGCTTAACACAA+TGG - chr4_4:81234806-81234825 None:intergenic 35.0%
! TTGTGTTAAGCCTTTTCCTT+TGG + chr4_4:81234806-81234825 Msa0707770:intron 35.0%
ACACACATAACCAAAGGAAA+AGG - chr4_4:81234819-81234838 None:intergenic 35.0%
CAATATGTAAGACGAAAGCA+TGG - chr4_4:81234859-81234878 None:intergenic 35.0%
TCGTCTTACATATTGTTTGC+TGG + chr4_4:81234864-81234883 Msa0707770:intron 35.0%
!!! GATCTCTGTTTGACTTGTTT+TGG + chr4_4:81234942-81234961 Msa0707770:intron 35.0%
!!! GACTTGTTTTGGTTTTTCTC+TGG + chr4_4:81234953-81234972 Msa0707770:intron 35.0%
ACTTCAGAACCAAATTTGCA+GGG - chr4_4:81234999-81235018 None:intergenic 35.0%
AACTTCAGAACCAAATTTGC+AGG - chr4_4:81235000-81235019 None:intergenic 35.0%
!! GCAAATTTGGTTCTGAAGTT+TGG + chr4_4:81235000-81235019 Msa0707770:intron 35.0%
!! AATTTGGTTCTGAAGTTTGG+TGG + chr4_4:81235003-81235022 Msa0707770:intron 35.0%
!! ATTTGGTTCTGAAGTTTGGT+GGG + chr4_4:81235004-81235023 Msa0707770:intron 35.0%
GATCTCGAATCAAAAGTGAT+CGG - chr4_4:81235030-81235049 None:intergenic 35.0%
!! GATCACTTTTGATTCGAGAT+CGG + chr4_4:81235029-81235048 Msa0707770:intron 35.0%
!! ATCACTTTTGATTCGAGATC+GGG + chr4_4:81235030-81235049 Msa0707770:intron 35.0%
!! TTTGACTCAAGTTGGTTCTT+GGG + chr4_4:81235062-81235081 Msa0707770:intron 35.0%
!!! GCTTTTGTTTTGTGCTCTTT+CGG + chr4_4:81235084-81235103 Msa0707770:intron 35.0%
TGTGTAAAATGCTTCAAAGC+TGG - chr4_4:81235458-81235477 None:intergenic 35.0%
! CATTTCTTTTCATCATGCCA+TGG - chr4_4:81235621-81235640 None:intergenic 35.0%
!! AAATAAATGGTGTTGCAGAC+TGG - chr4_4:81235752-81235771 None:intergenic 35.0%
!! TTTATGACAGTGTTGATCTG+CGG + chr4_4:81235916-81235935 Msa0707770:CDS 35.0%
GTAAAGGATCCTATGTCAAA+AGG - chr4_4:81236022-81236041 None:intergenic 35.0%
! TTAGAAATACCTTCTAGCTG+TGG - chr4_4:81236238-81236257 None:intergenic 35.0%
!! AGAGGCTTTGAAAGAAAAGC+GGG + chr4_4:81233038-81233057 Msa0707770:five_prime_UTR 40.0%
CTTCTTCTTCTTCTTCAGCT+CGG - chr4_4:81233084-81233103 None:intergenic 40.0%
AACAAGAGCTTGTAATCGAC+AGG - chr4_4:81233160-81233179 None:intergenic 40.0%
! TTTGTTGATTAGGTTACCGG+TGG + chr4_4:81233368-81233387 Msa0707770:intron 40.0%
AGCTTGTCCATTGAATCTCT+TGG + chr4_4:81233456-81233475 Msa0707770:CDS 40.0%
TGTCCATTGAATCTCTTGGT+TGG + chr4_4:81233460-81233479 Msa0707770:CDS 40.0%
GTCCATTGAATCTCTTGGTT+GGG + chr4_4:81233461-81233480 Msa0707770:CDS 40.0%
TCCATTGAATCTCTTGGTTG+GGG + chr4_4:81233462-81233481 Msa0707770:CDS 40.0%
TGAAAAAAAGACGCACCTTG+AGG - chr4_4:81233495-81233514 None:intergenic 40.0%
! AGTGGAGTAAGGCTCTTTTA+GGG - chr4_4:81233808-81233827 None:intergenic 40.0%
TAAAAGAGCCTTACTCCACT+GGG + chr4_4:81233808-81233827 Msa0707770:intron 40.0%
TTTACTTTCCCAGTGGAGTA+AGG - chr4_4:81233819-81233838 None:intergenic 40.0%
GTGTGTGTAGTGTACATTCT+CGG + chr4_4:81233852-81233871 Msa0707770:intron 40.0%
AGTTCAAACTCCCAAAGCAA+TGG - chr4_4:81233903-81233922 None:intergenic 40.0%
ACCTGTCATTCCCATATGAT+CGG + chr4_4:81234072-81234091 Msa0707770:intron 40.0%
ACGTAGATGCACAAATTCAC+AGG + chr4_4:81234135-81234154 Msa0707770:intron 40.0%
CGTAGATGCACAAATTCACA+GGG + chr4_4:81234136-81234155 Msa0707770:intron 40.0%
CCTGCCTAGTGTATTTGTTT+AGG - chr4_4:81234171-81234190 None:intergenic 40.0%
CCTAAACAAATACACTAGGC+AGG + chr4_4:81234168-81234187 Msa0707770:intron 40.0%
! ATACACTAGGCAGGTTTTCT+AGG + chr4_4:81234177-81234196 Msa0707770:intron 40.0%
! ATGGTTCATGTCAGCTTTAG+AGG - chr4_4:81234303-81234322 None:intergenic 40.0%
ACCCTGTCACTAACTATATG+AGG - chr4_4:81234367-81234386 None:intergenic 40.0%
CATATAGTTAGTGACAGGGT+TGG + chr4_4:81234367-81234386 Msa0707770:intron 40.0%
CAAAGGAAATCACCCAAACA+AGG - chr4_4:81234683-81234702 None:intergenic 40.0%
CTTGTTTGGGTGATTTCCTT+TGG + chr4_4:81234681-81234700 Msa0707770:intron 40.0%
AAACACCTAAACCACCCAAA+AGG - chr4_4:81234760-81234779 None:intergenic 40.0%
! TTGGGTGGTTTAGGTGTTTT+TGG + chr4_4:81234761-81234780 Msa0707770:intron 40.0%
!!! TAGGTGTTTTTGGAGTTTGG+AGG + chr4_4:81234771-81234790 Msa0707770:intron 40.0%
!!! AGGTGTTTTTGGAGTTTGGA+GGG + chr4_4:81234772-81234791 Msa0707770:intron 40.0%
!!! ATTGTTTGCTGGTTTTTCGG+TGG + chr4_4:81234875-81234894 Msa0707770:intron 40.0%
!!! TTGTTTGCTGGTTTTTCGGT+GGG + chr4_4:81234876-81234895 Msa0707770:intron 40.0%
!! TTGGTTTTTCTCTGGTCTAC+CGG + chr4_4:81234961-81234980 Msa0707770:intron 40.0%
GTCAGTTCTTTGACTCAAGT+TGG + chr4_4:81235054-81235073 Msa0707770:intron 40.0%
!! CTTTGACTCAAGTTGGTTCT+TGG + chr4_4:81235061-81235080 Msa0707770:intron 40.0%
!!! TTTGTTTTGTGCTCTTTCGG+TGG + chr4_4:81235087-81235106 Msa0707770:intron 40.0%
!! TTTCGAATTGTGGATTAGCC+TGG + chr4_4:81235160-81235179 Msa0707770:intron 40.0%
!! TTCGAATTGTGGATTAGCCT+GGG + chr4_4:81235161-81235180 Msa0707770:intron 40.0%
!!! TAGGTGTTGTTGTGTTTTGG+TGG + chr4_4:81235293-81235312 Msa0707770:intron 40.0%
!!! AGGTGTTGTTGTGTTTTGGT+GGG + chr4_4:81235294-81235313 Msa0707770:intron 40.0%
TTTCATGGGTGTTTCATAGG+TGG + chr4_4:81235322-81235341 Msa0707770:intron 40.0%
TATGGTGTACCGTCTATATG+CGG + chr4_4:81235346-81235365 Msa0707770:intron 40.0%
ATCCAGTGTTTGCAGAAATC+TGG + chr4_4:81235506-81235525 Msa0707770:intron 40.0%
TTCCAGATTTCTGCAAACAC+TGG - chr4_4:81235511-81235530 None:intergenic 40.0%
! TCTTTTCATCATGCCATGGA+TGG - chr4_4:81235617-81235636 None:intergenic 40.0%
TCAACACTGTCATAAAGGCA+TGG - chr4_4:81235912-81235931 None:intergenic 40.0%
GCAGATCAACACTGTCATAA+AGG - chr4_4:81235917-81235936 None:intergenic 40.0%
CAACTAGCATTTCCATGTGA+TGG + chr4_4:81235949-81235968 Msa0707770:CDS 40.0%
! ATCTTGTGGCCTTTTGACAT+AGG + chr4_4:81236010-81236029 Msa0707770:three_prime_UTR 40.0%
! ATTTTATCCCCAGACGATTC+TGG + chr4_4:81236099-81236118 Msa0707770:three_prime_UTR 40.0%
! TTTTATCCCCAGACGATTCT+GGG + chr4_4:81236100-81236119 Msa0707770:three_prime_UTR 40.0%
AAGTTATCCCAGAATCGTCT+GGG - chr4_4:81236110-81236129 None:intergenic 40.0%
TAAGTTATCCCAGAATCGTC+TGG - chr4_4:81236111-81236130 None:intergenic 40.0%
TCTTCATCCTTGCTTAACAC+AGG + chr4_4:81236307-81236326 Msa0707770:three_prime_UTR 40.0%
CTTGCTTAACACAGGTGAAT+GGG + chr4_4:81236315-81236334 Msa0707770:three_prime_UTR 40.0%
GTGAATGGGAAGGAAATACA+TGG + chr4_4:81236329-81236348 Msa0707770:three_prime_UTR 40.0%
GATATGTCAACTGCACATGA+CGG - chr4_4:81236375-81236394 None:intergenic 40.0%
!! GAGGCTTTGAAAGAAAAGCG+GGG + chr4_4:81233039-81233058 Msa0707770:five_prime_UTR 45.0%
AATGGCAACATCGTCGTGTA+AGG + chr4_4:81233119-81233138 Msa0707770:exon 45.0%
AACATCGTCGTGTAAGGCTT+TGG + chr4_4:81233125-81233144 Msa0707770:CDS 45.0%
ACATCGTCGTGTAAGGCTTT+GGG + chr4_4:81233126-81233145 Msa0707770:CDS 45.0%
GGTAGGTTAGAGAGAGAAGA+GGG - chr4_4:81233283-81233302 None:intergenic 45.0%
AGGTAGGTTAGAGAGAGAAG+AGG - chr4_4:81233284-81233303 None:intergenic 45.0%
ACCCCAACCAAGAGATTCAA+TGG - chr4_4:81233466-81233485 None:intergenic 45.0%
! CAGTGGAGTAAGGCTCTTTT+AGG - chr4_4:81233809-81233828 None:intergenic 45.0%
CTAAAAGAGCCTTACTCCAC+TGG + chr4_4:81233807-81233826 Msa0707770:intron 45.0%
TCCGATCATATGGGAATGAC+AGG - chr4_4:81234076-81234095 None:intergenic 45.0%
!! GTTCATGTCAGCTTTAGAGG+TGG - chr4_4:81234300-81234319 None:intergenic 45.0%
! GAGGTGATGATGTATGGTGA+TGG + chr4_4:81234631-81234650 Msa0707770:intron 45.0%
ACGAACAAGGACACGTGTAA+CGG - chr4_4:81234716-81234735 None:intergenic 45.0%
!!! TAGCTCTGGTTTGTGCCTTT+TGG + chr4_4:81234742-81234761 Msa0707770:intron 45.0%
!! AGCTCTGGTTTGTGCCTTTT+GGG + chr4_4:81234743-81234762 Msa0707770:intron 45.0%
!! TTGTGCCTTTTGGGTGGTTT+AGG + chr4_4:81234752-81234771 Msa0707770:intron 45.0%
GATCTCGAAGCCAAATGAAC+CGG - chr4_4:81234902-81234921 None:intergenic 45.0%
! GGTTCATTTGGCTTCGAGAT+CGG + chr4_4:81234901-81234920 Msa0707770:intron 45.0%
! GTTCATTTGGCTTCGAGATC+GGG + chr4_4:81234902-81234921 Msa0707770:intron 45.0%
! TGGTTTTTCTCTGGTCTACC+GGG + chr4_4:81234962-81234981 Msa0707770:intron 45.0%
! GTCAGGTGCTTTTCGAATTG+TGG + chr4_4:81235150-81235169 Msa0707770:intron 45.0%
GGGTGTTTCATAGGTGGTTA+TGG + chr4_4:81235328-81235347 Msa0707770:intron 45.0%
!! CAAATGGTGCCGCATATAGA+CGG - chr4_4:81235358-81235377 None:intergenic 45.0%
GAGAACATGGGTGACTAACT+AGG - chr4_4:81235564-81235583 None:intergenic 45.0%
TTCATCATGCCATGGATGGA+AGG - chr4_4:81235613-81235632 None:intergenic 45.0%
TTCCATGTGATGGCAGACTT+TGG + chr4_4:81235959-81235978 Msa0707770:exon 45.0%
AGTTATCCCAGAATCGTCTG+GGG - chr4_4:81236109-81236128 None:intergenic 45.0%
CCATTCACCTGTGTTAAGCA+AGG - chr4_4:81236317-81236336 None:intergenic 45.0%
CCTTGCTTAACACAGGTGAA+TGG + chr4_4:81236314-81236333 Msa0707770:three_prime_UTR 45.0%
CTTAACACAGGTGAATGGGA+AGG + chr4_4:81236319-81236338 Msa0707770:three_prime_UTR 45.0%
AAGCCTCTTCCTTCGATGCT+AGG - chr4_4:81233026-81233045 None:intergenic 50.0%
CGTGGAACAGTTGCAGATTG+AGG - chr4_4:81233234-81233253 None:intergenic 50.0%
TCCAAGCTACACAACTCCAC+CGG - chr4_4:81233387-81233406 None:intergenic 50.0%
AATGCTGCCCTTGTATAGCG+CGG + chr4_4:81233410-81233429 Msa0707770:CDS 50.0%
! GGTTGGGGTTTAGTTCCTCA+AGG + chr4_4:81233477-81233496 Msa0707770:CDS 50.0%
TGTTGGGTTTGACGTAAGGC+TGG + chr4_4:81234036-81234055 Msa0707770:intron 50.0%
GATGCACAAATTCACAGGGC+AGG + chr4_4:81234140-81234159 Msa0707770:intron 50.0%
!! CAGCTTTAGAGGTGGATCGA+GGG - chr4_4:81234292-81234311 None:intergenic 50.0%
!! TCAGCTTTAGAGGTGGATCG+AGG - chr4_4:81234293-81234312 None:intergenic 50.0%
!! GCTTTGAAGCATGCCATGTG+TGG + chr4_4:81234606-81234625 Msa0707770:intron 50.0%
TTGAAGCATGCCATGTGTGG+TGG + chr4_4:81234609-81234628 Msa0707770:intron 50.0%
ATCATCACCTCCACCACACA+TGG - chr4_4:81234622-81234641 None:intergenic 50.0%
! GTGGTGGAGGTGATGATGTA+TGG + chr4_4:81234625-81234644 Msa0707770:intron 50.0%
TTAGGAGGTGGGTCCTTGTT+TGG + chr4_4:81234667-81234686 Msa0707770:intron 50.0%
! TAGGAGGTGGGTCCTTGTTT+GGG + chr4_4:81234668-81234687 Msa0707770:intron 50.0%
! GTAACGGAGGTTGCTTCCAA+AGG - chr4_4:81234700-81234719 None:intergenic 50.0%
AACAAGGACACGTGTAACGG+AGG - chr4_4:81234713-81234732 None:intergenic 50.0%
AGAGCTAGTGACGACGAACA+AGG - chr4_4:81234729-81234748 None:intergenic 50.0%
TTGTTCGTCGTCACTAGCTC+TGG + chr4_4:81234728-81234747 Msa0707770:intron 50.0%
!! TCTGGTTTGTGCCTTTTGGG+TGG + chr4_4:81234746-81234765 Msa0707770:intron 50.0%
AGTCAAACAGAGATCGCAGC+TGG - chr4_4:81234937-81234956 None:intergenic 50.0%
! GGTTTTTCTCTGGTCTACCG+GGG + chr4_4:81234963-81234982 Msa0707770:intron 50.0%
! GTTTTTCTCTGGTCTACCGG+GGG + chr4_4:81234964-81234983 Msa0707770:intron 50.0%
TTGTGCTCTTTCGGTGGATC+AGG + chr4_4:81235093-81235112 Msa0707770:intron 50.0%
TGTGCTCTTTCGGTGGATCA+GGG + chr4_4:81235094-81235113 Msa0707770:intron 50.0%
!! TTCGAAAAGCACCTGACCGA+AGG - chr4_4:81235147-81235166 None:intergenic 50.0%
!! TGTGGATTAGCCTGGGTGTT+CGG + chr4_4:81235168-81235187 Msa0707770:intron 50.0%
TGGGGGAGAGTAGTGACAAT+AGG + chr4_4:81235221-81235240 Msa0707770:intron 50.0%
GGGAGAGTAGTGACAATAGG+CGG + chr4_4:81235224-81235243 Msa0707770:intron 50.0%
AGAGTAGTGACAATAGGCGG+TGG + chr4_4:81235227-81235246 Msa0707770:intron 50.0%
AGCCAAAGTCTGCCATCACA+TGG - chr4_4:81235964-81235983 None:intergenic 50.0%
CTGTGATCACCACAGCTAGA+AGG + chr4_4:81236226-81236245 Msa0707770:three_prime_UTR 50.0%
! GGTCCTAGCATCGAAGGAAG+AGG + chr4_4:81233020-81233039 Msa0707770:five_prime_UTR 55.0%
!! CGTCGTGTAAGGCTTTGGGT+AGG + chr4_4:81233130-81233149 Msa0707770:CDS 55.0%
CACGCGTCTCTCTCACTTCT+CGG + chr4_4:81233250-81233269 Msa0707770:CDS 55.0%
ACCGGTGGAGTTGTGTAGCT+TGG + chr4_4:81233383-81233402 Msa0707770:CDS 55.0%
GCTGAACAACACGTCTGCTG+TGG - chr4_4:81234444-81234463 None:intergenic 55.0%
AAGCATGCCATGTGTGGTGG+AGG + chr4_4:81234612-81234631 Msa0707770:intron 55.0%
!!! TTGCTGGTTTTTCGGTGGGC+CGG + chr4_4:81234880-81234899 Msa0707770:intron 55.0%
! TTTCGGTGGGCCGGTTCATT+TGG + chr4_4:81234889-81234908 Msa0707770:intron 55.0%
GTGCTCTTTCGGTGGATCAG+GGG + chr4_4:81235095-81235114 Msa0707770:intron 55.0%
GTGGCAGCTGCATCATCCTT+CGG + chr4_4:81235128-81235147 Msa0707770:intron 55.0%
AGCTGCATCATCCTTCGGTC+AGG + chr4_4:81235133-81235152 Msa0707770:intron 55.0%
!! GTGGATTAGCCTGGGTGTTC+GGG + chr4_4:81235169-81235188 Msa0707770:intron 55.0%
TAGCCTGGGTGTTCGGGTAT+AGG + chr4_4:81235175-81235194 Msa0707770:intron 55.0%
ACACCTATACCCGAACACCC+AGG - chr4_4:81235181-81235200 None:intergenic 55.0%
CGTTCATACCCTGCGTCTTG+CGG + chr4_4:81235388-81235407 Msa0707770:intron 55.0%
GTTCATACCCTGCGTCTTGC+GGG + chr4_4:81235389-81235408 Msa0707770:intron 55.0%
TTCATACCCTGCGTCTTGCG+GGG + chr4_4:81235390-81235409 Msa0707770:intron 55.0%
CCATGGATGGAAGGCTCTCT+CGG - chr4_4:81235604-81235623 None:intergenic 55.0%
CGAGAAGTGAGAGAGACGCG+TGG - chr4_4:81233252-81233271 None:intergenic 60.0%
GACGCGACCGCGCTATACAA+GGG - chr4_4:81233420-81233439 None:intergenic 60.0%
TGACGCGACCGCGCTATACA+AGG - chr4_4:81233421-81233440 None:intergenic 60.0%
!! CGAGGGGTGCCCTGCAAATT+TGG + chr4_4:81234987-81235006 Msa0707770:intron 60.0%
TGGATGGAAGGCTCTCTCGG+TGG - chr4_4:81235601-81235620 None:intergenic 60.0%
CCGAGAGAGCCTTCCATCCA+TGG + chr4_4:81235601-81235620 Msa0707770:intron 60.0%
!! GCGGGGTGACAGTGTCGCTT+AGG + chr4_4:81233056-81233075 Msa0707770:five_prime_UTR 65.0%
GGCTTCGAGATCGGGAGTCG+AGG + chr4_4:81234910-81234929 Msa0707770:intron 65.0%
GATCAGGGGTCGCGAGGTTG+TGG + chr4_4:81235109-81235128 Msa0707770:intron 65.0%
TCTCTGGTCTACCGGGGGCG+AGG + chr4_4:81234969-81234988 Msa0707770:intron 70.0%
CTCTGGTCTACCGGGGGCGA+GGG + chr4_4:81234970-81234989 Msa0707770:intron 70.0%
TCTGGTCTACCGGGGGCGAG+GGG + chr4_4:81234971-81234990 Msa0707770:intron 70.0%
TCGGTGGATCAGGGGTCGCG+AGG + chr4_4:81235103-81235122 Msa0707770:intron 70.0%
CGGCATCCCCGCAAGACGCA+GGG - chr4_4:81235399-81235418 None:intergenic 70.0%
ACGGCATCCCCGCAAGACGC+AGG - chr4_4:81235400-81235419 None:intergenic 70.0%
!! TGCAGGGCACCCCTCGCCCC+CGG - chr4_4:81234983-81235002 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 81233017 81236397 81233017 ID=Msa0707770;Name=Msa0707770
chr4_4 mRNA 81233017 81236397 81233017 ID=Msa0707770-mRNA-1;Parent=Msa0707770;Name=Msa0707770-mRNA-1;_AED=0.09;_eAED=0.09;_QI=103|0.33|0.75|0.75|0.33|0.5|4|429|132
chr4_4 exon 81233017 81233271 81233017 ID=Msa0707770-mRNA-1:exon:26527;Parent=Msa0707770-mRNA-1
chr4_4 exon 81233380 81233498 81233380 ID=Msa0707770-mRNA-1:exon:26528;Parent=Msa0707770-mRNA-1
chr4_4 exon 81235753 81235814 81235753 ID=Msa0707770-mRNA-1:exon:26529;Parent=Msa0707770-mRNA-1
chr4_4 exon 81235903 81236397 81235903 ID=Msa0707770-mRNA-1:exon:26530;Parent=Msa0707770-mRNA-1
chr4_4 five_prime_UTR 81233017 81233119 81233017 ID=Msa0707770-mRNA-1:five_prime_utr;Parent=Msa0707770-mRNA-1
chr4_4 CDS 81233120 81233271 81233120 ID=Msa0707770-mRNA-1:cds;Parent=Msa0707770-mRNA-1
chr4_4 CDS 81233380 81233498 81233380 ID=Msa0707770-mRNA-1:cds;Parent=Msa0707770-mRNA-1
chr4_4 CDS 81235753 81235814 81235753 ID=Msa0707770-mRNA-1:cds;Parent=Msa0707770-mRNA-1
chr4_4 CDS 81235903 81235968 81235903 ID=Msa0707770-mRNA-1:cds;Parent=Msa0707770-mRNA-1
chr4_4 three_prime_UTR 81235969 81236397 81235969 ID=Msa0707770-mRNA-1:three_prime_utr;Parent=Msa0707770-mRNA-1
Gene Sequence

>Msa0707770

ATGGCAACATCGTCGTGTAAGGCTTTGGGTAGGTTATCCTGTCGATTACAAGCTCTTGTTCATAATACCAACACTAAACAATCATTATTCTCTATCCATTTCAAACTCAAACCTCAATCTGCAACTGTTCCACGCGTCTCTCTCACTTCTCGGTTACCGGTGGAGTTGTGTAGCTTGGAATCAATGCTGCCCTTGTATAGCGCGGTCGCGTCAGCAAGATTAGTTTCAAGCTTGTCCATTGAATCTCTTGGTTGGGGTTTAGTTCCTCAAGTCTGCAACACCATTTATTTATCAAGAGTTATTCTTTTAAAGCACATAGTTGGTAAGATAATTGTATTTCCATGCCTTTATGACAGTGTTGATCTGCGGTTAGTTGATTCAACTAGCATTTCCATGTGA
Protein sequence

>Msa0707770

MATSSCKALGRLSCRLQALVHNTNTKQSLFSIHFKLKPQSATVPRVSLTSRLPVELCSLESMLPLYSAVASARLVSSLSIESLGWGLVPQVCNTIYLSRVILLKHIVGKIIVFPCLYDSVDLRLVDSTSISM*