AlfalfaGEDB | Msa0733380

M. sativa cultivar zhongmu-4 / Msa0733380

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006139 GO:0006417 GO:0006446 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009507 GO:0009536 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010608 GO:0010628 GO:0016070 GO:0016787 GO:0016788 GO:0019222 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032268 GO:0032270 GO:0034248 GO:0034250 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0045727 GO:0045948 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0060255 GO:0065007 GO:0070129 GO:0070131 GO:0070132 GO:0070134 GO:0071704 GO:0080090 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363 GO:2000112
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0733380	RHN56346.1	89.333	75	8	0	1	75	183	257	3.87e-40	146

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0733380	sp\|Q9M2A1\|PP263_ARATH	50.667	75	37	0	1	75	178	252	8.67e-18	80.5
Msa0733380	sp\|Q9M2A1\|PP263_ARATH	65.217	46	15	1	73	118	371	415	3.71e-12	64.7

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0733380	A0A396HSK8	89.333	75	8	0	1	75	183	257	1.85e-40	146

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0017510	Msa0733380	0.804873	1.752265e-49	-8.615850e-47
Msa0068680	Msa0733380	0.809734	1.634107e-50	-8.615850e-47
Msa0657970	Msa0733380	0.816953	4.239789e-52	-8.615850e-47
Msa0691380	Msa0733380	0.821510	3.885326e-53	-8.615850e-47
Msa0693380	Msa0733380	0.804076	2.569372e-49	-8.615850e-47
Msa0696640	Msa0733380	0.814116	1.814513e-51	-8.615850e-47
Msa0731940	Msa0733380	0.811436	7.008749e-51	-8.615850e-47
Msa0733360	Msa0733380	0.861848	7.687853e-64	-8.615850e-47
Msa0733380	Msa0742380	0.817939	2.541561e-52	-8.615850e-47
Msa0733380	Msa0744040	0.830453	2.911968e-55	-8.615850e-47
Msa0733380	Msa0745790	0.810529	1.101513e-50	-8.615850e-47
Msa0733380	Msa0777850	0.800750	1.244549e-48	-8.615850e-47
Msa0733380	Msa0790510	0.802248	6.139495e-49	-8.615850e-47
Msa0733380	Msa0810220	0.809270	2.056115e-50	-8.615850e-47
Msa0733380	Msa0824960	0.802640	5.098067e-49	-8.615850e-47
Msa0733380	Msa0880140	0.801877	7.317072e-49	-8.615850e-47
Msa0733380	Msa1014540	0.807608	4.651079e-50	-8.615850e-47
Msa0733380	Msa1059570	0.800902	1.158786e-48	-8.615850e-47
Msa0733380	Msa1082500	0.832412	9.594020e-56	-8.615850e-47
Msa0733380	Msa1270610	0.805408	1.354319e-49	-8.615850e-47
Msa0733380	Msa1283860	0.801218	9.987953e-49	-8.615850e-47
Msa0733380	Msa1322890	0.816244	6.110642e-52	-8.615850e-47
Msa0733380	Msa1329700	0.811638	6.333574e-51	-8.615850e-47
Msa0733380	Msa1372760	0.805913	1.061224e-49	-8.615850e-47
Msa0733380	Msa1392260	0.806828	6.805930e-50	-8.615850e-47
Msa0733380	Msa1411020	0.803758	2.991626e-49	-8.615850e-47
Msa0733380	Msa1424290	0.800219	1.596839e-48	-8.615850e-47
Msa0733380	Msa1427760	0.801215	1.000075e-48	-8.615850e-47
Msa0733380	Msa1434350	0.802520	5.395175e-49	-8.615850e-47
Msa0733380	Msa1439320	0.805262	1.453166e-49	-8.615850e-47
Msa0157290	Msa0733380	0.804279	2.330910e-49	-8.615850e-47
Msa0220580	Msa0733380	0.803467	3.438379e-49	-8.615850e-47
Msa0376590	Msa0733380	0.809414	1.914436e-50	-8.615850e-47
Msa0379640	Msa0733380	0.808325	3.273616e-50	-8.615850e-47
Msa0389340	Msa0733380	0.801181	1.016451e-48	-8.615850e-47
Msa0255550	Msa0733380	0.804654	1.947455e-49	-8.615850e-47
Msa0270100	Msa0733380	0.815410	9.380377e-52	-8.615850e-47
Msa0288100	Msa0733380	0.800720	1.262424e-48	-8.615850e-47
Msa0294590	Msa0733380	0.815492	8.990841e-52	-8.615850e-47
Msa0333030	Msa0733380	0.806212	9.177819e-50	-8.615850e-47
Msa0345970	Msa0733380	0.801046	1.082833e-48	-8.615850e-47
Msa0484780	Msa0733380	0.822302	2.546828e-53	-8.615850e-47
Msa0516370	Msa0733380	0.807455	5.013994e-50	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0733380	MtrunA17_Chr5g0428491	89.333	75	8	0	1	75	183	257	3.56e-44	146
Msa0733380	MtrunA17_Chr1g0171891	85.333	75	11	0	1	75	160	234	2.33e-39	135
Msa0733380	MtrunA17_Chr1g0171891	79.245	53	10	1	66	118	347	398	1.50e-21	87.8
Msa0733380	MtrunA17_Chr4g0020931	85.333	75	11	0	1	75	161	235	2.53e-39	135
Msa0733380	MtrunA17_Chr4g0020931	79.245	53	10	1	66	118	348	399	1.54e-21	87.8
Msa0733380	MtrunA17_Chr5g0428501	84.444	45	7	0	73	117	19	63	1.52e-22	83.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0733380	AT3G42630.1	50.667	75	37	0	1	75	178	252	8.81e-19	80.5
Msa0733380	AT3G42630.1	65.217	46	15	1	73	118	371	415	3.78e-13	64.7

Find 64 sgRNAs with CRISPR-Local

Find 187 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGAATTTCATTACCTGTTT+TGG	0.188813	5_1:-46604228	Msa0733370:intron
ATCGGATGGACCAAATATTT+TGG	0.206212	5_1:+46604677	Msa0733380:CDS
CTTGAGTTCGGAGGCACTTT+TGG	0.206401	5_1:+46604588	Msa0733380:CDS
TGCAAGTAGGATTGGTATTA+AGG	0.226390	5_1:+46605001	Msa0733380:three_prime_UTR
AATGAAATCGAAAATGCTTA+TGG	0.263652	5_1:+46604278	Msa0733380:CDS
TAGAGAAGCCAAGCAATTTC+AGG	0.263689	5_1:+46605170	Msa0733380:three_prime_UTR
GTTAGTAGTTCCAAAATATT+TGG	0.264609	5_1:-46604687	Msa0733370:intron
TTGAAACAAAGTTGTACTTA+TGG	0.274356	5_1:+46604755	Msa0733380:three_prime_UTR
AGTGATGGGGTTGATTTCTA+AGG	0.318887	5_1:+46605342	Msa0733380:three_prime_UTR
AACAACAGAAGTGGAGTTAC+AGG	0.322092	5_1:+46604623	Msa0733380:CDS
GTGATTAGGGCTATGGCTTA+TGG	0.328397	5_1:+46604338	Msa0733380:CDS
GATGGAGGAGAATGTGATTA+GGG	0.336753	5_1:+46604325	Msa0733380:CDS
TTTCAAATAGATTCCAAAAC+AGG	0.356714	5_1:+46604215	Msa0733380:CDS
ATGGAAGGTTTAAGAGATCT+AGG	0.371453	5_1:+46604297	Msa0733380:CDS
TGATGGAGGAGAATGTGATT+AGG	0.375774	5_1:+46604324	Msa0733380:CDS
AGAGTCAAGATCGGAGTCTA+TGG	0.381585	5_1:+46605119	Msa0733380:three_prime_UTR
TTTGTTAAAGATGTTGGTTT+AGG	0.403997	5_1:+46604398	Msa0733380:CDS
ATTCTATTCTTATGATATAG+AGG	0.404587	5_1:-46605431	Msa0733370:intron
TGCAGCATAATAATAGCCTT+TGG	0.406905	5_1:+46605389	Msa0733380:three_prime_UTR
GGTCAGTTTGTTAAAGATGT+TGG	0.415334	5_1:+46604392	Msa0733380:CDS
GAGGAGCTGTGAGTATTTGA+TGG	0.417334	5_1:+46605083	Msa0733380:three_prime_UTR
AATCAATGAAATGGATTCAA+AGG	0.425706	5_1:+46604190	None:intergenic
CAGTTTGCACCTGAAAATCC+TGG	0.443809	5_1:+46604928	Msa0733380:three_prime_UTR
GCAGCATAATAATAGCCTTT+GGG	0.453692	5_1:+46605390	Msa0733380:three_prime_UTR
ATTCAATCTTGAAGAATACT+TGG	0.475080	5_1:+46604872	Msa0733380:three_prime_UTR
GGAGAATATTCATGAGCGTT+GGG	0.478607	5_1:+46605140	Msa0733380:three_prime_UTR
ATCAATGAAATGGATTCAAA+GGG	0.483362	5_1:+46604191	None:intergenic
CCATCCGATACTGTTTCATA+AGG	0.484003	5_1:-46604663	Msa0733370:intron
TGGAGAATATTCATGAGCGT+TGG	0.489355	5_1:+46605139	Msa0733380:three_prime_UTR
CCTTGACACTTCATCTTCAA+AGG	0.492938	5_1:-46604784	Msa0733370:intron
CATAATGATTATGAATTCCC+AGG	0.499640	5_1:-46604946	Msa0733370:intron
GCACTACATGCAAGTAGGAT+TGG	0.511124	5_1:+46604993	Msa0733380:three_prime_UTR
GGAGAATGTGATTAGGGCTA+TGG	0.512451	5_1:+46604331	Msa0733380:CDS
GAGTCACTAATAGTAATGTA+AGG	0.521457	5_1:+46605281	Msa0733380:three_prime_UTR
ATTTCAGGAAAACCAAGAAA+AGG	0.524893	5_1:+46605185	Msa0733380:three_prime_UTR
ATCAAATACTCACAGCTCCT+CGG	0.529676	5_1:-46605081	Msa0733370:intron
GATATAGAGGATTGAATGAG+TGG	0.534175	5_1:-46605418	Msa0733370:intron
ACAAGACACAACAACAGAAG+TGG	0.543259	5_1:+46604614	Msa0733380:CDS
ATTTGAAACAGAGATTCAAA+AGG	0.549963	5_1:+46605250	Msa0733380:three_prime_UTR
AAATCGAAAATGCTTATGGA+AGG	0.550904	5_1:+46604282	Msa0733380:CDS
GTGCTGCACTACATGCAAGT+AGG	0.554310	5_1:+46604988	Msa0733380:three_prime_UTR
CGATCTTGACTCTGTTCTTG+TGG	0.555214	5_1:-46605109	Msa0733370:intron
AAACAAAGTTGTACTTATGG+TGG	0.565170	5_1:+46604758	Msa0733380:three_prime_UTR
TAGTTAAACATTAAAACTAC+AGG	0.565872	5_1:-46605515	Msa0733370:intron
CGCAGGTGTGGCATGATGCA+CGG	0.571923	5_1:+46605220	Msa0733380:three_prime_UTR
AATACTCACAGCTCCTCGGA+GGG	0.574542	5_1:-46605077	Msa0733370:intron
GGTGAAACTGAGAGACGGTA+TGG	0.583932	5_1:+46604893	Msa0733380:three_prime_UTR
TGAAGTAGTTGCCGCAGGTG+TGG	0.593173	5_1:+46605208	Msa0733380:three_prime_UTR
AATACCTTATGAAACAGTAT+CGG	0.594966	5_1:+46604659	Msa0733380:CDS
ACAACAGAAGTGGAGTTACA+GGG	0.598915	5_1:+46604624	Msa0733380:CDS
AAAGGTGAAGTAGTTGCCGC+AGG	0.605600	5_1:+46605203	Msa0733380:three_prime_UTR
CCTTATGAAACAGTATCGGA+TGG	0.607484	5_1:+46604663	Msa0733380:CDS
AGTTTGCACCTGAAAATCCT+GGG	0.607831	5_1:+46604929	Msa0733380:three_prime_UTR
CACAAGAACAGAGTCAAGAT+CGG	0.611703	5_1:+46605110	Msa0733380:three_prime_UTR
TGTAGTGCAGCACAAAGTGT+AGG	0.617181	5_1:-46604978	Msa0733370:intron
CGTGCATCATGCCACACCTG+CGG	0.632126	5_1:-46605219	Msa0733370:intron
ATACTCACAGCTCCTCGGAG+GGG	0.655007	5_1:-46605076	Msa0733370:intron
TACTTGGTGAAACTGAGAGA+CGG	0.661616	5_1:+46604888	Msa0733380:three_prime_UTR
AAATACTCACAGCTCCTCGG+AGG	0.674552	5_1:-46605078	Msa0733370:intron
GAATGAGTGGACATACCCAA+AGG	0.679320	5_1:-46605405	Msa0733370:intron
CATAACACTGAATGTGTGCG+TGG	0.681315	5_1:-46604724	Msa0733370:intron
GCGGTTTCAGAGCCCCTCCG+AGG	0.686700	5_1:+46605064	Msa0733380:three_prime_UTR
TTGAAGATGAAGTGTCAAGG+TGG	0.694209	5_1:+46604787	Msa0733380:three_prime_UTR
CCTTTGAAGATGAAGTGTCA+AGG	0.700877	5_1:+46604784	Msa0733380:three_prime_UTR

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TAAAAAATAGAGTTTAATTT+TGG	-	chr5_1:46605320-46605339	Msa0733370:intron	10.0%
!!!	TAAAAAATAGAGTTTAATTT+TGG	-	chr5_1:46605320-46605339	Msa0733370:intron	10.0%
!!!	AAGAGAAAATTTTATGAGTT+AGG	+	chr5_1:46604371-46604390	Msa0733380:CDS	20.0%
!!!	TTTGTTTTGAATAAAATGGA+TGG	+	chr5_1:46604498-46604517	Msa0733380:intron	20.0%
!!!	ACTCTATTTTTTATTAGTGA+TGG	+	chr5_1:46605327-46605346	Msa0733380:three_prime_UTR	20.0%
!!!	CTCTATTTTTTATTAGTGAT+GGG	+	chr5_1:46605328-46605347	Msa0733380:three_prime_UTR	20.0%
!!!	TCTATTTTTTATTAGTGATG+GGG	+	chr5_1:46605329-46605348	Msa0733380:three_prime_UTR	20.0%
!!	ATTCTATTCTTATGATATAG+AGG	-	chr5_1:46605434-46605453	Msa0733370:intron	20.0%
!!	TAGTTAAACATTAAAACTAC+AGG	-	chr5_1:46605518-46605537	Msa0733370:intron	20.0%
!!!	AAGAGAAAATTTTATGAGTT+AGG	+	chr5_1:46604371-46604390	Msa0733380:CDS	20.0%
!!!	TTTGTTTTGAATAAAATGGA+TGG	+	chr5_1:46604498-46604517	Msa0733380:intron	20.0%
!!!	ACTCTATTTTTTATTAGTGA+TGG	+	chr5_1:46605327-46605346	Msa0733380:three_prime_UTR	20.0%
!!!	CTCTATTTTTTATTAGTGAT+GGG	+	chr5_1:46605328-46605347	Msa0733380:three_prime_UTR	20.0%
!!!	TCTATTTTTTATTAGTGATG+GGG	+	chr5_1:46605329-46605348	Msa0733380:three_prime_UTR	20.0%
!!	ATTCTATTCTTATGATATAG+AGG	-	chr5_1:46605434-46605453	Msa0733370:intron	20.0%
!!	TAGTTAAACATTAAAACTAC+AGG	-	chr5_1:46605518-46605537	Msa0733370:intron	20.0%
!	TTTCAAATAGATTCCAAAAC+AGG	+	chr5_1:46604215-46604234	Msa0733380:CDS	25.0%
!!	AAGAATTTCATTACCTGTTT+TGG	-	chr5_1:46604231-46604250	Msa0733370:intron	25.0%
!!	CTTATGTGTTATAGTGTTTT+TGG	+	chr5_1:46604248-46604267	Msa0733380:CDS	25.0%
!	AATGAAATCGAAAATGCTTA+TGG	+	chr5_1:46604278-46604297	Msa0733380:CDS	25.0%
!!!	TTTGTTAAAGATGTTGGTTT+AGG	+	chr5_1:46604398-46604417	Msa0733380:CDS	25.0%
!!!	GGAGTTTGTTTTGAATAAAA+TGG	+	chr5_1:46604494-46604513	Msa0733380:intron	25.0%
!	AATACCTTATGAAACAGTAT+CGG	+	chr5_1:46604659-46604678	Msa0733380:CDS	25.0%
!	GTTAGTAGTTCCAAAATATT+TGG	-	chr5_1:46604690-46604709	Msa0733370:intron	25.0%
!	TTGAAACAAAGTTGTACTTA+TGG	+	chr5_1:46604755-46604774	Msa0733380:three_prime_UTR	25.0%
!	ATTCAATCTTGAAGAATACT+TGG	+	chr5_1:46604872-46604891	Msa0733380:three_prime_UTR	25.0%
!!	ATTTGAAACAGAGATTCAAA+AGG	+	chr5_1:46605250-46605269	Msa0733380:three_prime_UTR	25.0%
!	TTTCAAATAGATTCCAAAAC+AGG	+	chr5_1:46604215-46604234	Msa0733380:CDS	25.0%
!!	AAGAATTTCATTACCTGTTT+TGG	-	chr5_1:46604231-46604250	Msa0733370:intron	25.0%
!!	CTTATGTGTTATAGTGTTTT+TGG	+	chr5_1:46604248-46604267	Msa0733380:CDS	25.0%
!	AATGAAATCGAAAATGCTTA+TGG	+	chr5_1:46604278-46604297	Msa0733380:CDS	25.0%
!!!	TTTGTTAAAGATGTTGGTTT+AGG	+	chr5_1:46604398-46604417	Msa0733380:CDS	25.0%
!!!	GGAGTTTGTTTTGAATAAAA+TGG	+	chr5_1:46604494-46604513	Msa0733380:intron	25.0%
!	AATACCTTATGAAACAGTAT+CGG	+	chr5_1:46604659-46604678	Msa0733380:CDS	25.0%
!	GTTAGTAGTTCCAAAATATT+TGG	-	chr5_1:46604690-46604709	Msa0733370:intron	25.0%
!	TTGAAACAAAGTTGTACTTA+TGG	+	chr5_1:46604755-46604774	Msa0733380:three_prime_UTR	25.0%
!	ATTCAATCTTGAAGAATACT+TGG	+	chr5_1:46604872-46604891	Msa0733380:three_prime_UTR	25.0%
!!	ATTTGAAACAGAGATTCAAA+AGG	+	chr5_1:46605250-46605269	Msa0733380:three_prime_UTR	25.0%
	AAATCGAAAATGCTTATGGA+AGG	+	chr5_1:46604282-46604301	Msa0733380:CDS	30.0%
!	TAAGAGATCTAGGTTTTTGA+TGG	+	chr5_1:46604307-46604326	Msa0733380:CDS	30.0%
!!	TTAAAGATGTTGGTTTAGGT+AGG	+	chr5_1:46604402-46604421	Msa0733380:intron	30.0%
!!	GTTTTGAATAAAATGGATGG+TGG	+	chr5_1:46604501-46604520	Msa0733380:intron	30.0%
	AAACAAAGTTGTACTTATGG+TGG	+	chr5_1:46604758-46604777	Msa0733380:three_prime_UTR	30.0%
	CATAATGATTATGAATTCCC+AGG	-	chr5_1:46604949-46604968	Msa0733370:intron	30.0%
	ATTTCAGGAAAACCAAGAAA+AGG	+	chr5_1:46605185-46605204	Msa0733380:three_prime_UTR	30.0%
	GAGTCACTAATAGTAATGTA+AGG	+	chr5_1:46605281-46605300	Msa0733380:three_prime_UTR	30.0%
	AAATCGAAAATGCTTATGGA+AGG	+	chr5_1:46604282-46604301	Msa0733380:CDS	30.0%
!	TAAGAGATCTAGGTTTTTGA+TGG	+	chr5_1:46604307-46604326	Msa0733380:CDS	30.0%
!!	TTAAAGATGTTGGTTTAGGT+AGG	+	chr5_1:46604402-46604421	Msa0733380:intron	30.0%
!!	GTTTTGAATAAAATGGATGG+TGG	+	chr5_1:46604501-46604520	Msa0733380:intron	30.0%
	AAACAAAGTTGTACTTATGG+TGG	+	chr5_1:46604758-46604777	Msa0733380:three_prime_UTR	30.0%
	CATAATGATTATGAATTCCC+AGG	-	chr5_1:46604949-46604968	Msa0733370:intron	30.0%
	ATTTCAGGAAAACCAAGAAA+AGG	+	chr5_1:46605185-46605204	Msa0733380:three_prime_UTR	30.0%
	GAGTCACTAATAGTAATGTA+AGG	+	chr5_1:46605281-46605300	Msa0733380:three_prime_UTR	30.0%
	ATGGAAGGTTTAAGAGATCT+AGG	+	chr5_1:46604297-46604316	Msa0733380:CDS	35.0%
!!	GGTCAGTTTGTTAAAGATGT+TGG	+	chr5_1:46604392-46604411	Msa0733380:CDS	35.0%
	GTTTAGGTAGGAGAAATGTA+GGG	+	chr5_1:46604414-46604433	Msa0733380:intron	35.0%
!	GAAATGTAGGGAATCTTTTG+TGG	+	chr5_1:46604426-46604445	Msa0733380:intron	35.0%
!	AATCTTTTGTGGTTGTGTTG+TGG	+	chr5_1:46604437-46604456	Msa0733380:intron	35.0%
!	TCGTTTGTGTTTGACGTTTT+AGG	+	chr5_1:46604550-46604569	Msa0733380:intron	35.0%
!!	GTGTTTGACGTTTTAGGTAA+AGG	+	chr5_1:46604556-46604575	Msa0733380:intron	35.0%
!	AGGTGATTTTCACTTGAGTT+CGG	+	chr5_1:46604576-46604595	Msa0733380:CDS	35.0%
!	ATCGGATGGACCAAATATTT+TGG	+	chr5_1:46604677-46604696	Msa0733380:CDS	35.0%
!	TATGAATTCCCAGGATTTTC+AGG	-	chr5_1:46604940-46604959	Msa0733370:intron	35.0%
!	TGCAAGTAGGATTGGTATTA+AGG	+	chr5_1:46605001-46605020	Msa0733380:three_prime_UTR	35.0%
!!	TCTGAACGAGTTTTATCAAG+CGG	+	chr5_1:46605045-46605064	Msa0733380:three_prime_UTR	35.0%
!!	TTGGTTTTCCTGAAATTGCT+TGG	-	chr5_1:46605181-46605200	Msa0733370:intron	35.0%
	TGCAGCATAATAATAGCCTT+TGG	+	chr5_1:46605389-46605408	Msa0733380:three_prime_UTR	35.0%
	GCAGCATAATAATAGCCTTT+GGG	+	chr5_1:46605390-46605409	Msa0733380:three_prime_UTR	35.0%
	GATATAGAGGATTGAATGAG+TGG	-	chr5_1:46605421-46605440	Msa0733370:intron	35.0%
	TGAAAACCCTTTGAATCCAT+TGG	-	chr5_1:46604200-46604219	Msa0733370:intron	35.0%
	ATGGAAGGTTTAAGAGATCT+AGG	+	chr5_1:46604297-46604316	Msa0733380:CDS	35.0%
!!	GGTCAGTTTGTTAAAGATGT+TGG	+	chr5_1:46604392-46604411	Msa0733380:CDS	35.0%
	GTTTAGGTAGGAGAAATGTA+GGG	+	chr5_1:46604414-46604433	Msa0733380:intron	35.0%
!	GAAATGTAGGGAATCTTTTG+TGG	+	chr5_1:46604426-46604445	Msa0733380:intron	35.0%
!	AATCTTTTGTGGTTGTGTTG+TGG	+	chr5_1:46604437-46604456	Msa0733380:intron	35.0%
!	TCGTTTGTGTTTGACGTTTT+AGG	+	chr5_1:46604550-46604569	Msa0733380:intron	35.0%
!!	GTGTTTGACGTTTTAGGTAA+AGG	+	chr5_1:46604556-46604575	Msa0733380:intron	35.0%
!	AGGTGATTTTCACTTGAGTT+CGG	+	chr5_1:46604576-46604595	Msa0733380:CDS	35.0%
!	ATCGGATGGACCAAATATTT+TGG	+	chr5_1:46604677-46604696	Msa0733380:CDS	35.0%
!	TATGAATTCCCAGGATTTTC+AGG	-	chr5_1:46604940-46604959	Msa0733370:intron	35.0%
!	TGCAAGTAGGATTGGTATTA+AGG	+	chr5_1:46605001-46605020	Msa0733380:three_prime_UTR	35.0%
!!	TCTGAACGAGTTTTATCAAG+CGG	+	chr5_1:46605045-46605064	Msa0733380:three_prime_UTR	35.0%
!!	TTGGTTTTCCTGAAATTGCT+TGG	-	chr5_1:46605181-46605200	Msa0733370:intron	35.0%
	TGCAGCATAATAATAGCCTT+TGG	+	chr5_1:46605389-46605408	Msa0733380:three_prime_UTR	35.0%
	GCAGCATAATAATAGCCTTT+GGG	+	chr5_1:46605390-46605409	Msa0733380:three_prime_UTR	35.0%
	GATATAGAGGATTGAATGAG+TGG	-	chr5_1:46605421-46605440	Msa0733370:intron	35.0%
!	GAGATCTAGGTTTTTGATGG+AGG	+	chr5_1:46604310-46604329	Msa0733380:CDS	40.0%
	TGATGGAGGAGAATGTGATT+AGG	+	chr5_1:46604324-46604343	Msa0733380:CDS	40.0%
	GATGGAGGAGAATGTGATTA+GGG	+	chr5_1:46604325-46604344	Msa0733380:CDS	40.0%
	GGTTTAGGTAGGAGAAATGT+AGG	+	chr5_1:46604413-46604432	Msa0733380:intron	40.0%
	TTGTGTTGTGGATGCTTACT+TGG	+	chr5_1:46604449-46604468	Msa0733380:intron	40.0%
	TGGATGCTTACTTGGATAGA+AGG	+	chr5_1:46604457-46604476	Msa0733380:intron	40.0%
	TAGAAGGCTTGGAAGGAATT+TGG	+	chr5_1:46604473-46604492	Msa0733380:intron	40.0%
!	TGATTTTCACTTGAGTTCGG+AGG	+	chr5_1:46604579-46604598	Msa0733380:CDS	40.0%
	ACAAGACACAACAACAGAAG+TGG	+	chr5_1:46604614-46604633	Msa0733380:CDS	40.0%
	AACAACAGAAGTGGAGTTAC+AGG	+	chr5_1:46604623-46604642	Msa0733380:CDS	40.0%
	ACAACAGAAGTGGAGTTACA+GGG	+	chr5_1:46604624-46604643	Msa0733380:CDS	40.0%
	CCATCCGATACTGTTTCATA+AGG	-	chr5_1:46604666-46604685	Msa0733370:intron	40.0%
!	CCTTATGAAACAGTATCGGA+TGG	+	chr5_1:46604663-46604682	Msa0733380:CDS	40.0%
	CCTTGACACTTCATCTTCAA+AGG	-	chr5_1:46604787-46604806	Msa0733370:intron	40.0%
!	CCTTTGAAGATGAAGTGTCA+AGG	+	chr5_1:46604784-46604803	Msa0733380:three_prime_UTR	40.0%
!	TTGAAGATGAAGTGTCAAGG+TGG	+	chr5_1:46604787-46604806	Msa0733380:three_prime_UTR	40.0%
!	TACTTGGTGAAACTGAGAGA+CGG	+	chr5_1:46604888-46604907	Msa0733380:three_prime_UTR	40.0%
	AGTTTGCACCTGAAAATCCT+GGG	+	chr5_1:46604929-46604948	Msa0733380:three_prime_UTR	40.0%
	ATCAAATACTCACAGCTCCT+CGG	-	chr5_1:46605084-46605103	Msa0733370:intron	40.0%
	CACAAGAACAGAGTCAAGAT+CGG	+	chr5_1:46605110-46605129	Msa0733380:three_prime_UTR	40.0%
	TGGAGAATATTCATGAGCGT+TGG	+	chr5_1:46605139-46605158	Msa0733380:three_prime_UTR	40.0%
	GGAGAATATTCATGAGCGTT+GGG	+	chr5_1:46605140-46605159	Msa0733380:three_prime_UTR	40.0%
	TAGAGAAGCCAAGCAATTTC+AGG	+	chr5_1:46605170-46605189	Msa0733380:three_prime_UTR	40.0%
!	GCAACTACTTCACCTTTTCT+TGG	-	chr5_1:46605200-46605219	Msa0733370:intron	40.0%
!	AGTGATGGGGTTGATTTCTA+AGG	+	chr5_1:46605342-46605361	Msa0733380:three_prime_UTR	40.0%
!	GAGATCTAGGTTTTTGATGG+AGG	+	chr5_1:46604310-46604329	Msa0733380:CDS	40.0%
	TGATGGAGGAGAATGTGATT+AGG	+	chr5_1:46604324-46604343	Msa0733380:CDS	40.0%
	GATGGAGGAGAATGTGATTA+GGG	+	chr5_1:46604325-46604344	Msa0733380:CDS	40.0%
	GGTTTAGGTAGGAGAAATGT+AGG	+	chr5_1:46604413-46604432	Msa0733380:intron	40.0%
	TTGTGTTGTGGATGCTTACT+TGG	+	chr5_1:46604449-46604468	Msa0733380:intron	40.0%
	TGGATGCTTACTTGGATAGA+AGG	+	chr5_1:46604457-46604476	Msa0733380:intron	40.0%
	TAGAAGGCTTGGAAGGAATT+TGG	+	chr5_1:46604473-46604492	Msa0733380:intron	40.0%
!	TGATTTTCACTTGAGTTCGG+AGG	+	chr5_1:46604579-46604598	Msa0733380:CDS	40.0%
	ACAAGACACAACAACAGAAG+TGG	+	chr5_1:46604614-46604633	Msa0733380:CDS	40.0%
	AACAACAGAAGTGGAGTTAC+AGG	+	chr5_1:46604623-46604642	Msa0733380:CDS	40.0%
	ACAACAGAAGTGGAGTTACA+GGG	+	chr5_1:46604624-46604643	Msa0733380:CDS	40.0%
	CCATCCGATACTGTTTCATA+AGG	-	chr5_1:46604666-46604685	Msa0733370:intron	40.0%
!	CCTTATGAAACAGTATCGGA+TGG	+	chr5_1:46604663-46604682	Msa0733380:CDS	40.0%
	CCTTGACACTTCATCTTCAA+AGG	-	chr5_1:46604787-46604806	Msa0733370:intron	40.0%
!	CCTTTGAAGATGAAGTGTCA+AGG	+	chr5_1:46604784-46604803	Msa0733380:three_prime_UTR	40.0%
!	TTGAAGATGAAGTGTCAAGG+TGG	+	chr5_1:46604787-46604806	Msa0733380:three_prime_UTR	40.0%
!	TACTTGGTGAAACTGAGAGA+CGG	+	chr5_1:46604888-46604907	Msa0733380:three_prime_UTR	40.0%
	AGTTTGCACCTGAAAATCCT+GGG	+	chr5_1:46604929-46604948	Msa0733380:three_prime_UTR	40.0%
	ATCAAATACTCACAGCTCCT+CGG	-	chr5_1:46605084-46605103	Msa0733370:intron	40.0%
	CACAAGAACAGAGTCAAGAT+CGG	+	chr5_1:46605110-46605129	Msa0733380:three_prime_UTR	40.0%
	TGGAGAATATTCATGAGCGT+TGG	+	chr5_1:46605139-46605158	Msa0733380:three_prime_UTR	40.0%
	GGAGAATATTCATGAGCGTT+GGG	+	chr5_1:46605140-46605159	Msa0733380:three_prime_UTR	40.0%
	TAGAGAAGCCAAGCAATTTC+AGG	+	chr5_1:46605170-46605189	Msa0733380:three_prime_UTR	40.0%
!	GCAACTACTTCACCTTTTCT+TGG	-	chr5_1:46605200-46605219	Msa0733370:intron	40.0%
!	AGTGATGGGGTTGATTTCTA+AGG	+	chr5_1:46605342-46605361	Msa0733380:three_prime_UTR	40.0%
	GGAGAATGTGATTAGGGCTA+TGG	+	chr5_1:46604331-46604350	Msa0733380:CDS	45.0%
	GTGATTAGGGCTATGGCTTA+TGG	+	chr5_1:46604338-46604357	Msa0733380:CDS	45.0%
	GCTTACTTGGATAGAAGGCT+TGG	+	chr5_1:46604462-46604481	Msa0733380:intron	45.0%
	ACTTGGATAGAAGGCTTGGA+AGG	+	chr5_1:46604466-46604485	Msa0733380:intron	45.0%
	AACGAATCAGTCAACATCCG+CGG	-	chr5_1:46604535-46604554	Msa0733370:intron	45.0%
	CATAACACTGAATGTGTGCG+TGG	-	chr5_1:46604727-46604746	Msa0733370:intron	45.0%
	CAGTTTGCACCTGAAAATCC+TGG	+	chr5_1:46604928-46604947	Msa0733380:three_prime_UTR	45.0%
	TGTAGTGCAGCACAAAGTGT+AGG	-	chr5_1:46604981-46605000	Msa0733370:intron	45.0%
	GCACTACATGCAAGTAGGAT+TGG	+	chr5_1:46604993-46605012	Msa0733380:three_prime_UTR	45.0%
	GAGGAGCTGTGAGTATTTGA+TGG	+	chr5_1:46605083-46605102	Msa0733380:three_prime_UTR	45.0%
	CGATCTTGACTCTGTTCTTG+TGG	-	chr5_1:46605112-46605131	Msa0733370:intron	45.0%
	AGAGTCAAGATCGGAGTCTA+TGG	+	chr5_1:46605119-46605138	Msa0733380:three_prime_UTR	45.0%
	GAATGAGTGGACATACCCAA+AGG	-	chr5_1:46605408-46605427	Msa0733370:intron	45.0%
	GGAGAATGTGATTAGGGCTA+TGG	+	chr5_1:46604331-46604350	Msa0733380:CDS	45.0%
	GTGATTAGGGCTATGGCTTA+TGG	+	chr5_1:46604338-46604357	Msa0733380:CDS	45.0%
	GCTTACTTGGATAGAAGGCT+TGG	+	chr5_1:46604462-46604481	Msa0733380:intron	45.0%
	ACTTGGATAGAAGGCTTGGA+AGG	+	chr5_1:46604466-46604485	Msa0733380:intron	45.0%
	AACGAATCAGTCAACATCCG+CGG	-	chr5_1:46604535-46604554	Msa0733370:intron	45.0%
	CATAACACTGAATGTGTGCG+TGG	-	chr5_1:46604727-46604746	Msa0733370:intron	45.0%
	CAGTTTGCACCTGAAAATCC+TGG	+	chr5_1:46604928-46604947	Msa0733380:three_prime_UTR	45.0%
	TGTAGTGCAGCACAAAGTGT+AGG	-	chr5_1:46604981-46605000	Msa0733370:intron	45.0%
	GCACTACATGCAAGTAGGAT+TGG	+	chr5_1:46604993-46605012	Msa0733380:three_prime_UTR	45.0%
	GAGGAGCTGTGAGTATTTGA+TGG	+	chr5_1:46605083-46605102	Msa0733380:three_prime_UTR	45.0%
	CGATCTTGACTCTGTTCTTG+TGG	-	chr5_1:46605112-46605131	Msa0733370:intron	45.0%
	AGAGTCAAGATCGGAGTCTA+TGG	+	chr5_1:46605119-46605138	Msa0733380:three_prime_UTR	45.0%
	GAATGAGTGGACATACCCAA+AGG	-	chr5_1:46605408-46605427	Msa0733370:intron	45.0%
!!!	CTTGAGTTCGGAGGCACTTT+TGG	+	chr5_1:46604588-46604607	Msa0733380:CDS	50.0%
	GGTGAAACTGAGAGACGGTA+TGG	+	chr5_1:46604893-46604912	Msa0733380:three_prime_UTR	50.0%
	GTGCTGCACTACATGCAAGT+AGG	+	chr5_1:46604988-46605007	Msa0733380:three_prime_UTR	50.0%
	AATACTCACAGCTCCTCGGA+GGG	-	chr5_1:46605080-46605099	Msa0733370:intron	50.0%
	AAATACTCACAGCTCCTCGG+AGG	-	chr5_1:46605081-46605100	Msa0733370:intron	50.0%
	AAAGGTGAAGTAGTTGCCGC+AGG	+	chr5_1:46605203-46605222	Msa0733380:three_prime_UTR	50.0%
!!!	CTTGAGTTCGGAGGCACTTT+TGG	+	chr5_1:46604588-46604607	Msa0733380:CDS	50.0%
	GGTGAAACTGAGAGACGGTA+TGG	+	chr5_1:46604893-46604912	Msa0733380:three_prime_UTR	50.0%
	GTGCTGCACTACATGCAAGT+AGG	+	chr5_1:46604988-46605007	Msa0733380:three_prime_UTR	50.0%
	AATACTCACAGCTCCTCGGA+GGG	-	chr5_1:46605080-46605099	Msa0733370:intron	50.0%
	AAATACTCACAGCTCCTCGG+AGG	-	chr5_1:46605081-46605100	Msa0733370:intron	50.0%
	AAAGGTGAAGTAGTTGCCGC+AGG	+	chr5_1:46605203-46605222	Msa0733380:three_prime_UTR	50.0%
!!	GGATGGTGGATGATTGTCCG+CGG	+	chr5_1:46604515-46604534	Msa0733380:intron	55.0%
	ATACTCACAGCTCCTCGGAG+GGG	-	chr5_1:46605079-46605098	Msa0733370:intron	55.0%
	TGAAGTAGTTGCCGCAGGTG+TGG	+	chr5_1:46605208-46605227	Msa0733380:three_prime_UTR	55.0%
!!	GGATGGTGGATGATTGTCCG+CGG	+	chr5_1:46604515-46604534	Msa0733380:intron	55.0%
	ATACTCACAGCTCCTCGGAG+GGG	-	chr5_1:46605079-46605098	Msa0733370:intron	55.0%
	TGAAGTAGTTGCCGCAGGTG+TGG	+	chr5_1:46605208-46605227	Msa0733380:three_prime_UTR	55.0%
	CGTGCATCATGCCACACCTG+CGG	-	chr5_1:46605222-46605241	Msa0733370:intron	60.0%
	CGCAGGTGTGGCATGATGCA+CGG	+	chr5_1:46605220-46605239	Msa0733380:three_prime_UTR	60.0%
	CGTGCATCATGCCACACCTG+CGG	-	chr5_1:46605222-46605241	Msa0733370:intron	60.0%
	CGCAGGTGTGGCATGATGCA+CGG	+	chr5_1:46605220-46605239	Msa0733380:three_prime_UTR	60.0%
	GCGGTTTCAGAGCCCCTCCG+AGG	+	chr5_1:46605064-46605083	Msa0733380:three_prime_UTR	70.0%
	GCGGTTTCAGAGCCCCTCCG+AGG	+	chr5_1:46605064-46605083	Msa0733380:three_prime_UTR	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr5_1	gene	46604200	46605557	46604200	ID=Msa0733380;Name=Msa0733380
chr5_1	mRNA	46604200	46605557	46604200	ID=Msa0733380-mRNA-1;Parent=Msa0733380;Name=Msa0733380-mRNA-1;_AED=0.42;_eAED=0.42;_QI=0\|0\|0.5\|1\|1\|1\|2\|849\|118
chr5_1	exon	46604200	46604419	46604200	ID=Msa0733380-mRNA-1:exon:13290;Parent=Msa0733380-mRNA-1
chr5_1	exon	46604572	46605557	46604572	ID=Msa0733380-mRNA-1:exon:13291;Parent=Msa0733380-mRNA-1
chr5_1	CDS	46604200	46604419	46604200	ID=Msa0733380-mRNA-1:cds;Parent=Msa0733380-mRNA-1
chr5_1	CDS	46604572	46604708	46604572	ID=Msa0733380-mRNA-1:cds;Parent=Msa0733380-mRNA-1
chr5_1	three_prime_UTR	46604709	46605557	46604709	ID=Msa0733380-mRNA-1:three_prime_utr;Parent=Msa0733380-mRNA-1

Gene Sequence	>Msa0733380 ATGGATTCAAAGGGTTTTCAAATAGATTCCAAAACAGGTAATGAAATTCTTATGTGTTATAGTGTTTTTGGTAGTTTGAATGAAATCGAAAATGCTTATGGAAGGTTTAAGAGATCTAGGTTTTTGATGGAGGAGAATGTGATTAGGGCTATGGCTTATGGTTATATAAAGAAGAGAAAATTTTATGAGTTAGGTCAGTTTGTTAAAGATGTTGGTTTAGGTAAAGGTGATTTTCACTTGAGTTCGGAGGCACTTTTGGAATACAAGACACAACAACAGAAGTGGAGTTACAGGGTTTTGATAAAGAAATACCTTATGAAACAGTATCGGATGGACCAAATATTTTGGAACTACTAA
Protein sequence	>Msa0733380 MDSKGFQIDSKTGNEILMCYSVFGSLNEIENAYGRFKRSRFLMEENVIRAMAYGYIKKRKFYELGQFVKDVGLGKGDFHLSSEALLEYKTQQQKWSYRVLIKKYLMKQYRMDQIFWNY*