AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0742500


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0742500 MtrunA17_Chr5g0444201 99.091 110 1 0 1 110 1 110 3.63e-78 225
Msa0742500 MtrunA17_Chr3g0098971 84.404 109 17 0 2 110 4 112 3.07e-65 192
Msa0742500 MtrunA17_Chr4g0024171 71.560 109 30 1 3 110 5 113 9.29e-56 168
Msa0742500 MtrunA17_Chr2g0319561 72.072 111 29 1 2 110 4 114 6.68e-55 166
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0742500 AT5G54940.1 81.651 109 20 0 2 110 4 112 6.80e-64 189
Msa0742500 AT5G54940.3 81.651 109 20 0 2 110 4 112 6.80e-64 189
Msa0742500 AT5G54940.2 81.651 109 20 0 2 110 4 112 6.80e-64 189
Msa0742500 AT1G54290.1 72.727 110 29 1 2 110 4 113 8.44e-58 174
Msa0742500 AT4G27130.1 73.636 110 28 1 2 110 4 113 1.13e-57 174
Msa0742500 AT5G54760.2 73.636 110 28 1 2 110 4 113 1.94e-57 173
Msa0742500 AT5G54760.4 73.636 110 28 1 2 110 4 113 1.94e-57 173
Msa0742500 AT5G54760.3 73.636 110 28 1 2 110 4 113 1.94e-57 173
Msa0742500 AT5G54760.1 73.636 110 28 1 2 110 4 113 1.94e-57 173

Find 29 sgRNAs with CRISPR-Local

Find 126 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTCTTTCTTGAGATCTTTA+AGG 0.098306 5_1:+62419716 None:intergenic
ACCTGGTGCATCTGATTCTT+TGG 0.208720 5_1:+62419837 None:intergenic
CAAAAGAGTATGTGCATATT+AGG 0.278072 5_1:-62419813 Msa0742500:CDS
TCAGTCCTTGCACTGTTGTT+AGG 0.278405 5_1:+62419764 None:intergenic
TAGAAGAGAGTTGAAGAGAT+TGG 0.333969 5_1:+62420894 None:intergenic
AAGGACAACATCAAGATTCA+TGG 0.385779 5_1:-62419580 Msa0742500:CDS
GATTCTTTGGCCTCAGCAAA+TGG 0.405574 5_1:+62419850 None:intergenic
GTTGTGAATTGCAAGGATCT+TGG 0.420337 5_1:-62419676 Msa0742500:CDS
AGAAGAGAGTTGAAGAGATT+GGG 0.422313 5_1:+62420895 None:intergenic
TCATGCTGGTCTTGCTAGAA+AGG 0.426864 5_1:-62419599 Msa0742500:CDS
AGTTGAAGAGATTGGGAATG+TGG 0.436578 5_1:+62420902 None:intergenic
TATCTCTATCTGCAGACTAC+AGG 0.445137 5_1:-62420853 Msa0742500:intron
TGCGTTGATCACCTTGGAGT+TGG 0.445951 5_1:+62419644 None:intergenic
TCTCACTTCCTCATTCATGC+TGG 0.461083 5_1:-62419613 Msa0742500:CDS
GCAAATGGATCAAATGTGCT+TGG 0.480564 5_1:+62419865 None:intergenic
AGAGATTGGGAATGTGGAGG+TGG 0.498637 5_1:+62420908 None:intergenic
CATTCTTGCGTTGATCACCT+TGG 0.510216 5_1:+62419638 None:intergenic
CAAATGGATCAAATGTGCTT+GGG 0.511747 5_1:+62419866 None:intergenic
CACATTTGATCCATTTGCTG+AGG 0.534407 5_1:-62419860 Msa0742500:CDS
ATTAGGATACAACAAAGGAA+TGG 0.567289 5_1:-62419796 Msa0742500:CDS
GAGATTGGGAATGTGGAGGT+GGG 0.587196 5_1:+62420909 None:intergenic
TAGCAAGACCAGCATGAATG+AGG 0.588886 5_1:+62419605 None:intergenic
AATCACAAAGAAAGTGTACA+TGG 0.596673 5_1:-62419902 Msa0742500:exon
GGAAAGATTATCCAACTCCA+AGG 0.612457 5_1:-62419655 Msa0742500:CDS
GCCAAAGAATCAGATGCACC+AGG 0.626824 5_1:-62419838 Msa0742500:CDS
AAGAGCCTAACAACAGTGCA+AGG 0.631107 5_1:-62419769 Msa0742500:CDS
TGGAAATGTTGTGAATTGCA+AGG 0.663364 5_1:-62419683 Msa0742500:CDS
TGAAGAGATTGGGAATGTGG+AGG 0.681590 5_1:+62420905 None:intergenic
TGCATATTAGGATACAACAA+AGG 0.686189 5_1:-62419801 Msa0742500:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAAGAAAAAAAATTAAGAA+GGG + chr5_1:62420023-62420042 None:intergenic 10.0%
!! TTTAAGAAAAAAAATTAAGA+AGG + chr5_1:62420024-62420043 None:intergenic 10.0%
!! TCATATATATAATTCAAAAT+TGG + chr5_1:62420407-62420426 None:intergenic 10.0%
!! TTAAGAAAAAAAATTAAGAA+GGG + chr5_1:62420023-62420042 None:intergenic 10.0%
!! TTTAAGAAAAAAAATTAAGA+AGG + chr5_1:62420024-62420043 None:intergenic 10.0%
!! TCATATATATAATTCAAAAT+TGG + chr5_1:62420407-62420426 None:intergenic 10.0%
!! GGGTAAATTATTATTAATAA+CGG + chr5_1:62420003-62420022 None:intergenic 15.0%
!! ATGTAATAAAATAAGATGTT+TGG + chr5_1:62420189-62420208 None:intergenic 15.0%
!! GGGTAAATTATTATTAATAA+CGG + chr5_1:62420003-62420022 None:intergenic 15.0%
!! ATGTAATAAAATAAGATGTT+TGG + chr5_1:62420189-62420208 None:intergenic 15.0%
!! CTTTCATATATAGTTAATCA+TGG - chr5_1:62419881-62419900 Msa0742500:CDS 20.0%
!! ATATTTACAATTGTTCCATA+TGG + chr5_1:62419968-62419987 None:intergenic 20.0%
!! ATCTAGGTTATTAGAATTAA+TGG + chr5_1:62420221-62420240 None:intergenic 20.0%
!! AAACATAATCGTTAAAACTA+AGG - chr5_1:62420457-62420476 Msa0742500:intron 20.0%
!! CTTTCATATATAGTTAATCA+TGG - chr5_1:62419881-62419900 Msa0742500:CDS 20.0%
!! ATATTTACAATTGTTCCATA+TGG + chr5_1:62419968-62419987 None:intergenic 20.0%
!! ATCTAGGTTATTAGAATTAA+TGG + chr5_1:62420221-62420240 None:intergenic 20.0%
!! AAACATAATCGTTAAAACTA+AGG - chr5_1:62420457-62420476 Msa0742500:intron 20.0%
! AATATACTCCAAGTTTGATA+TGG - chr5_1:62419733-62419752 Msa0742500:CDS 25.0%
! AATATTCTCCAAGTTTGATA+TGG - chr5_1:62419818-62419837 Msa0742500:CDS 25.0%
! ATATTCTCCAAGTTTGATAT+GGG - chr5_1:62419819-62419838 Msa0742500:CDS 25.0%
! AATATAACTAGGAATATGAG+TGG - chr5_1:62420341-62420360 Msa0742500:intron 25.0%
!!! ATGTGAATGAATGATTTGTT+TGG - chr5_1:62420426-62420445 Msa0742500:intron 25.0%
! CAGATTAAAACCAAATCAAA+AGG + chr5_1:62420485-62420504 None:intergenic 25.0%
! ACAAAACAACACCAAAATTA+AGG + chr5_1:62420542-62420561 None:intergenic 25.0%
! AATATACTCCAAGTTTGATA+TGG - chr5_1:62419733-62419752 Msa0742500:CDS 25.0%
! AATATTCTCCAAGTTTGATA+TGG - chr5_1:62419818-62419837 Msa0742500:CDS 25.0%
! ATATTCTCCAAGTTTGATAT+GGG - chr5_1:62419819-62419838 Msa0742500:CDS 25.0%
! AATATAACTAGGAATATGAG+TGG - chr5_1:62420341-62420360 Msa0742500:intron 25.0%
!!! ATGTGAATGAATGATTTGTT+TGG - chr5_1:62420426-62420445 Msa0742500:intron 25.0%
! CAGATTAAAACCAAATCAAA+AGG + chr5_1:62420485-62420504 None:intergenic 25.0%
! ACAAAACAACACCAAAATTA+AGG + chr5_1:62420542-62420561 None:intergenic 25.0%
TAGAATCACCATATCAAACT+TGG + chr5_1:62419744-62419763 None:intergenic 30.0%
TATTCTCCAAGTTTGATATG+GGG - chr5_1:62419820-62419839 Msa0742500:CDS 30.0%
TATAATCCCCATATCAAACT+TGG + chr5_1:62419829-62419848 None:intergenic 30.0%
! TAATCATGGTTTTCGACAAT+AGG - chr5_1:62419895-62419914 Msa0742500:exon 30.0%
! AGTTTGAGTTTGTAACCTTA+AGG - chr5_1:62419927-62419946 Msa0742500:intron 30.0%
AATTCAGACATAGTTCCTTA+AGG + chr5_1:62419945-62419964 None:intergenic 30.0%
AACTATGTCTGAATTCCATA+TGG - chr5_1:62419950-62419969 Msa0742500:intron 30.0%
! AATCACAAAGAAAGTGTACA+TGG - chr5_1:62420580-62420599 Msa0742500:intron 30.0%
! CAAAAGAGTATGTGCATATT+AGG - chr5_1:62420669-62420688 Msa0742500:intron 30.0%
TGCATATTAGGATACAACAA+AGG - chr5_1:62420681-62420700 Msa0742500:intron 30.0%
ATTAGGATACAACAAAGGAA+TGG - chr5_1:62420686-62420705 Msa0742500:intron 30.0%
ACTCTTTCTTGAGATCTTTA+AGG + chr5_1:62420769-62420788 None:intergenic 30.0%
!!! AAGAAAGAGTTTTGTTGCAA+TGG - chr5_1:62420779-62420798 Msa0742500:intron 30.0%
TAGAATCACCATATCAAACT+TGG + chr5_1:62419744-62419763 None:intergenic 30.0%
TATTCTCCAAGTTTGATATG+GGG - chr5_1:62419820-62419839 Msa0742500:CDS 30.0%
TATAATCCCCATATCAAACT+TGG + chr5_1:62419829-62419848 None:intergenic 30.0%
! TAATCATGGTTTTCGACAAT+AGG - chr5_1:62419895-62419914 Msa0742500:exon 30.0%
! AGTTTGAGTTTGTAACCTTA+AGG - chr5_1:62419927-62419946 Msa0742500:intron 30.0%
AATTCAGACATAGTTCCTTA+AGG + chr5_1:62419945-62419964 None:intergenic 30.0%
AACTATGTCTGAATTCCATA+TGG - chr5_1:62419950-62419969 Msa0742500:intron 30.0%
! AATCACAAAGAAAGTGTACA+TGG - chr5_1:62420580-62420599 Msa0742500:intron 30.0%
! CAAAAGAGTATGTGCATATT+AGG - chr5_1:62420669-62420688 Msa0742500:intron 30.0%
TGCATATTAGGATACAACAA+AGG - chr5_1:62420681-62420700 Msa0742500:intron 30.0%
ATTAGGATACAACAAAGGAA+TGG - chr5_1:62420686-62420705 Msa0742500:intron 30.0%
ACTCTTTCTTGAGATCTTTA+AGG + chr5_1:62420769-62420788 None:intergenic 30.0%
!!! AAGAAAGAGTTTTGTTGCAA+TGG - chr5_1:62420779-62420798 Msa0742500:intron 30.0%
AGAAGAGAGTTGAAGAGATT+GGG + chr5_1:62419590-62419609 None:intergenic 35.0%
TAGAAGAGAGTTGAAGAGAT+TGG + chr5_1:62419591-62419610 None:intergenic 35.0%
ATAACCTAGATCCTTGCAAT+CGG - chr5_1:62420230-62420249 Msa0742500:intron 35.0%
!! AACTAAGGAGCCTTTTGATT+TGG - chr5_1:62420472-62420491 Msa0742500:intron 35.0%
CAAATGGATCAAATGTGCTT+GGG + chr5_1:62420619-62420638 None:intergenic 35.0%
TGGAAATGTTGTGAATTGCA+AGG - chr5_1:62420799-62420818 Msa0742500:intron 35.0%
AAGGACAACATCAAGATTCA+TGG - chr5_1:62420902-62420921 Msa0742500:five_prime_UTR 35.0%
AGAAGAGAGTTGAAGAGATT+GGG + chr5_1:62419590-62419609 None:intergenic 35.0%
TAGAAGAGAGTTGAAGAGAT+TGG + chr5_1:62419591-62419610 None:intergenic 35.0%
ATAACCTAGATCCTTGCAAT+CGG - chr5_1:62420230-62420249 Msa0742500:intron 35.0%
!! AACTAAGGAGCCTTTTGATT+TGG - chr5_1:62420472-62420491 Msa0742500:intron 35.0%
CAAATGGATCAAATGTGCTT+GGG + chr5_1:62420619-62420638 None:intergenic 35.0%
TGGAAATGTTGTGAATTGCA+AGG - chr5_1:62420799-62420818 Msa0742500:intron 35.0%
AAGGACAACATCAAGATTCA+TGG - chr5_1:62420902-62420921 Msa0742500:five_prime_UTR 35.0%
AGTTGAAGAGATTGGGAATG+TGG + chr5_1:62419583-62419602 None:intergenic 40.0%
TATCTCTATCTGCAGACTAC+AGG - chr5_1:62419629-62419648 Msa0742500:CDS 40.0%
ATTAAGGTGCATGCATGCAT+GGG + chr5_1:62420526-62420545 None:intergenic 40.0%
AATTAAGGTGCATGCATGCA+TGG + chr5_1:62420527-62420546 None:intergenic 40.0%
! GCATGCATGCACCTTAATTT+TGG - chr5_1:62420528-62420547 Msa0742500:intron 40.0%
GCAAATGGATCAAATGTGCT+TGG + chr5_1:62420620-62420639 None:intergenic 40.0%
! CACATTTGATCCATTTGCTG+AGG - chr5_1:62420622-62420641 Msa0742500:intron 40.0%
GTTGTGAATTGCAAGGATCT+TGG - chr5_1:62420806-62420825 Msa0742500:intron 40.0%
GGAAAGATTATCCAACTCCA+AGG - chr5_1:62420827-62420846 Msa0742500:intron 40.0%
AGTTGAAGAGATTGGGAATG+TGG + chr5_1:62419583-62419602 None:intergenic 40.0%
TATCTCTATCTGCAGACTAC+AGG - chr5_1:62419629-62419648 Msa0742500:CDS 40.0%
ATTAAGGTGCATGCATGCAT+GGG + chr5_1:62420526-62420545 None:intergenic 40.0%
AATTAAGGTGCATGCATGCA+TGG + chr5_1:62420527-62420546 None:intergenic 40.0%
! GCATGCATGCACCTTAATTT+TGG - chr5_1:62420528-62420547 Msa0742500:intron 40.0%
GCAAATGGATCAAATGTGCT+TGG + chr5_1:62420620-62420639 None:intergenic 40.0%
! CACATTTGATCCATTTGCTG+AGG - chr5_1:62420622-62420641 Msa0742500:intron 40.0%
GTTGTGAATTGCAAGGATCT+TGG - chr5_1:62420806-62420825 Msa0742500:intron 40.0%
GGAAAGATTATCCAACTCCA+AGG - chr5_1:62420827-62420846 Msa0742500:intron 40.0%
TGAAGAGATTGGGAATGTGG+AGG + chr5_1:62419580-62419599 None:intergenic 45.0%
TCTTCCGATTGCAAGGATCT+AGG + chr5_1:62420237-62420256 None:intergenic 45.0%
GTGTACTTCTTCCGATTGCA+AGG + chr5_1:62420244-62420263 None:intergenic 45.0%
GATTCTTTGGCCTCAGCAAA+TGG + chr5_1:62420635-62420654 None:intergenic 45.0%
ACCTGGTGCATCTGATTCTT+TGG + chr5_1:62420648-62420667 None:intergenic 45.0%
! TGCACATACTCTTTTGCACC+TGG + chr5_1:62420665-62420684 None:intergenic 45.0%
AAGAGCCTAACAACAGTGCA+AGG - chr5_1:62420713-62420732 Msa0742500:intron 45.0%
TCAGTCCTTGCACTGTTGTT+AGG + chr5_1:62420721-62420740 None:intergenic 45.0%
! CATTCTTGCGTTGATCACCT+TGG + chr5_1:62420847-62420866 None:intergenic 45.0%
TCTCACTTCCTCATTCATGC+TGG - chr5_1:62420869-62420888 Msa0742500:five_prime_UTR 45.0%
TAGCAAGACCAGCATGAATG+AGG + chr5_1:62420880-62420899 None:intergenic 45.0%
!! TCATGCTGGTCTTGCTAGAA+AGG - chr5_1:62420883-62420902 Msa0742500:five_prime_UTR 45.0%
TGAAGAGATTGGGAATGTGG+AGG + chr5_1:62419580-62419599 None:intergenic 45.0%
TCTTCCGATTGCAAGGATCT+AGG + chr5_1:62420237-62420256 None:intergenic 45.0%
GTGTACTTCTTCCGATTGCA+AGG + chr5_1:62420244-62420263 None:intergenic 45.0%
GATTCTTTGGCCTCAGCAAA+TGG + chr5_1:62420635-62420654 None:intergenic 45.0%
ACCTGGTGCATCTGATTCTT+TGG + chr5_1:62420648-62420667 None:intergenic 45.0%
! TGCACATACTCTTTTGCACC+TGG + chr5_1:62420665-62420684 None:intergenic 45.0%
AAGAGCCTAACAACAGTGCA+AGG - chr5_1:62420713-62420732 Msa0742500:intron 45.0%
TCAGTCCTTGCACTGTTGTT+AGG + chr5_1:62420721-62420740 None:intergenic 45.0%
! CATTCTTGCGTTGATCACCT+TGG + chr5_1:62420847-62420866 None:intergenic 45.0%
TCTCACTTCCTCATTCATGC+TGG - chr5_1:62420869-62420888 Msa0742500:five_prime_UTR 45.0%
TAGCAAGACCAGCATGAATG+AGG + chr5_1:62420880-62420899 None:intergenic 45.0%
!! TCATGCTGGTCTTGCTAGAA+AGG - chr5_1:62420883-62420902 Msa0742500:five_prime_UTR 45.0%
!!! GTTATATTTTAAAAATAATT+TGG + chr5_1:62420329-62420348 None:intergenic 5.0%
!!! ATTATTTTTAAAATATAACT+AGG - chr5_1:62420330-62420349 Msa0742500:intron 5.0%
!!! GTTATATTTTAAAAATAATT+TGG + chr5_1:62420329-62420348 None:intergenic 5.0%
!!! ATTATTTTTAAAATATAACT+AGG - chr5_1:62420330-62420349 Msa0742500:intron 5.0%
GAGATTGGGAATGTGGAGGT+GGG + chr5_1:62419576-62419595 None:intergenic 50.0%
AGAGATTGGGAATGTGGAGG+TGG + chr5_1:62419577-62419596 None:intergenic 50.0%
GCCAAAGAATCAGATGCACC+AGG - chr5_1:62420644-62420663 Msa0742500:intron 50.0%
! TGCGTTGATCACCTTGGAGT+TGG + chr5_1:62420841-62420860 None:intergenic 50.0%
GAGATTGGGAATGTGGAGGT+GGG + chr5_1:62419576-62419595 None:intergenic 50.0%
AGAGATTGGGAATGTGGAGG+TGG + chr5_1:62419577-62419596 None:intergenic 50.0%
GCCAAAGAATCAGATGCACC+AGG - chr5_1:62420644-62420663 Msa0742500:intron 50.0%
! TGCGTTGATCACCTTGGAGT+TGG + chr5_1:62420841-62420860 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
chr5_1 gene 62419573 62420931 62419573 ID=Msa0742500;Name=Msa0742500
chr5_1 mRNA 62419573 62420931 62419573 ID=Msa0742500-mRNA-1;Parent=Msa0742500;Name=Msa0742500-mRNA-1;_AED=0.15;_eAED=0.21;_QI=101|0|0.5|1|0|0|2|0|110
chr5_1 exon 62419573 62419928 62419573 ID=Msa0742500-mRNA-1:exon:18340;Parent=Msa0742500-mRNA-1
chr5_1 exon 62420854 62420931 62420854 ID=Msa0742500-mRNA-1:exon:18339;Parent=Msa0742500-mRNA-1
chr5_1 five_prime_UTR 62420854 62420931 62420854 ID=Msa0742500-mRNA-1:five_prime_utr;Parent=Msa0742500-mRNA-1
chr5_1 five_prime_UTR 62419906 62419928 62419906 ID=Msa0742500-mRNA-1:five_prime_utr;Parent=Msa0742500-mRNA-1
chr5_1 CDS 62419573 62419905 62419573 ID=Msa0742500-mRNA-1:cds;Parent=Msa0742500-mRNA-1
Gene Sequence

>Msa0742500

ATGGTTGATATTCAGATCCCAAGCACATTTGATCCATTTGCTGAGGCCAAAGAATCAGATGCACCAGGTGCAAAAGAGTATGTGCATATTAGGATACAACAAAGGAATGGAAAAAAGAGCCTAACAACAGTGCAAGGACTGAAAAAAGAGTTCAGCTATGAGAAGATCCTTAAAGATCTCAAGAAAGAGTTTTGTTGCAATGGAAATGTTGTGAATTGCAAGGATCTTGGAAAGATTATCCAACTCCAAGGTGATCAACGCAAGAATGTTTCTCACTTCCTCATTCATGCTGGTCTTGCTAGAAAGGACAACATCAAGATTCATGGTTTTTAA

Protein sequence

>Msa0742500

MVDIQIPSTFDPFAEAKESDAPGAKEYVHIRIQQRNGKKSLTTVQGLKKEFSYEKILKDLKKEFCCNGNVVNCKDLGKIIQLQGDQRKNVSHFLIHAGLARKDNIKIHGF*