AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0779540


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0779540 MtrunA17_Chr5g0444201 99.091 110 1 0 1 110 1 110 3.63e-78 225
Msa0779540 MtrunA17_Chr3g0098971 84.404 109 17 0 2 110 4 112 3.07e-65 192
Msa0779540 MtrunA17_Chr4g0024171 71.560 109 30 1 3 110 5 113 9.29e-56 168
Msa0779540 MtrunA17_Chr2g0319561 72.072 111 29 1 2 110 4 114 6.68e-55 166
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0779540 AT5G54940.1 81.651 109 20 0 2 110 4 112 6.80e-64 189
Msa0779540 AT5G54940.3 81.651 109 20 0 2 110 4 112 6.80e-64 189
Msa0779540 AT5G54940.2 81.651 109 20 0 2 110 4 112 6.80e-64 189
Msa0779540 AT1G54290.1 72.727 110 29 1 2 110 4 113 8.44e-58 174
Msa0779540 AT4G27130.1 73.636 110 28 1 2 110 4 113 1.13e-57 174
Msa0779540 AT5G54760.2 73.636 110 28 1 2 110 4 113 1.94e-57 173
Msa0779540 AT5G54760.4 73.636 110 28 1 2 110 4 113 1.94e-57 173
Msa0779540 AT5G54760.3 73.636 110 28 1 2 110 4 113 1.94e-57 173
Msa0779540 AT5G54760.1 73.636 110 28 1 2 110 4 113 1.94e-57 173

Find 29 sgRNAs with CRISPR-Local

Find 126 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTCTTTCTTGAGATCTTTA+AGG 0.098306 5_2:+60788947 None:intergenic
ACCTGGTGCATCTGATTCTT+TGG 0.208720 5_2:+60789068 None:intergenic
CAAAAGAGTATGTGCATATT+AGG 0.278072 5_2:-60789044 Msa0779540:CDS
TCAGTCCTTGCACTGTTGTT+AGG 0.278405 5_2:+60788995 None:intergenic
TAGAAGAGAGTTGAAGAGAT+TGG 0.333969 5_2:+60790161 None:intergenic
AAGGACAACATCAAGATTCA+TGG 0.385779 5_2:-60788811 Msa0779540:CDS
GATTCTTTGGCCTCAGCAAA+TGG 0.405574 5_2:+60789081 None:intergenic
GTTGTGAATTGCAAGGATCT+TGG 0.420337 5_2:-60788907 Msa0779540:CDS
AGAAGAGAGTTGAAGAGATT+GGG 0.422313 5_2:+60790162 None:intergenic
TCATGCTGGTCTTGCTAGAA+AGG 0.426864 5_2:-60788830 Msa0779540:CDS
AGTTGAAGAGATTGGGAATG+TGG 0.436578 5_2:+60790169 None:intergenic
TATCTCTATCTGCAGACTAC+AGG 0.445137 5_2:-60790120 Msa0779540:intron
TGCGTTGATCACCTTGGAGT+TGG 0.445951 5_2:+60788875 None:intergenic
TCTCACTTCCTCATTCATGC+TGG 0.461083 5_2:-60788844 Msa0779540:CDS
GCAAATGGATCAAATGTGCT+TGG 0.480564 5_2:+60789096 None:intergenic
AGAGATTGGGAATGTGGAGG+TGG 0.498637 5_2:+60790175 None:intergenic
CATTCTTGCGTTGATCACCT+TGG 0.510216 5_2:+60788869 None:intergenic
CAAATGGATCAAATGTGCTT+GGG 0.511747 5_2:+60789097 None:intergenic
CACATTTGATCCATTTGCTG+AGG 0.534407 5_2:-60789091 Msa0779540:CDS
ATTAGGATACAACAAAGGAA+TGG 0.567289 5_2:-60789027 Msa0779540:CDS
GAGATTGGGAATGTGGAGGT+GGG 0.587196 5_2:+60790176 None:intergenic
TAGCAAGACCAGCATGAATG+AGG 0.588886 5_2:+60788836 None:intergenic
AATCACAAAGAAAGTGTACA+TGG 0.596673 5_2:-60789133 Msa0779540:exon
GGAAAGATTATCCAACTCCA+AGG 0.612457 5_2:-60788886 Msa0779540:CDS
GCCAAAGAATCAGATGCACC+AGG 0.626824 5_2:-60789069 Msa0779540:CDS
AAGAGCCTAACAACAGTGCA+AGG 0.631107 5_2:-60789000 Msa0779540:CDS
TGGAAATGTTGTGAATTGCA+AGG 0.663364 5_2:-60788914 Msa0779540:CDS
TGAAGAGATTGGGAATGTGG+AGG 0.681590 5_2:+60790172 None:intergenic
TGCATATTAGGATACAACAA+AGG 0.686189 5_2:-60789032 Msa0779540:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCATATATATAATTCAAAAT+TGG + chr5_2:60789674-60789693 None:intergenic 10.0%
!! TCATATATATAATTCAAAAT+TGG + chr5_2:60789674-60789693 None:intergenic 10.0%
!! GGGTAAATTATTATTAATAA+CGG + chr5_2:60789233-60789252 None:intergenic 15.0%
!! AGAAAAAAAATTATGAAGAA+GGG + chr5_2:60789253-60789272 None:intergenic 15.0%
!! AAGAAAAAAAATTATGAAGA+AGG + chr5_2:60789254-60789273 None:intergenic 15.0%
!! ATGTAATAAAATAAGATGTT+TGG + chr5_2:60789422-60789441 None:intergenic 15.0%
!! GGGTAAATTATTATTAATAA+CGG + chr5_2:60789233-60789252 None:intergenic 15.0%
!! AGAAAAAAAATTATGAAGAA+GGG + chr5_2:60789253-60789272 None:intergenic 15.0%
!! AAGAAAAAAAATTATGAAGA+AGG + chr5_2:60789254-60789273 None:intergenic 15.0%
!! ATGTAATAAAATAAGATGTT+TGG + chr5_2:60789422-60789441 None:intergenic 15.0%
!! CTTTCATATATAGTTAATCA+TGG - chr5_2:60789111-60789130 Msa0779540:CDS 20.0%
!! ATATTTACAATTGTTCCATA+TGG + chr5_2:60789198-60789217 None:intergenic 20.0%
!! ATCTAGGTTATTAGAATTAA+TGG + chr5_2:60789454-60789473 None:intergenic 20.0%
!! AAACATAATCGTTAAAACTA+AGG - chr5_2:60789724-60789743 Msa0779540:intron 20.0%
!! CTTTCATATATAGTTAATCA+TGG - chr5_2:60789111-60789130 Msa0779540:CDS 20.0%
!! ATATTTACAATTGTTCCATA+TGG + chr5_2:60789198-60789217 None:intergenic 20.0%
!! ATCTAGGTTATTAGAATTAA+TGG + chr5_2:60789454-60789473 None:intergenic 20.0%
!! AAACATAATCGTTAAAACTA+AGG - chr5_2:60789724-60789743 Msa0779540:intron 20.0%
! AATATACTCCAAGTTTGATA+TGG - chr5_2:60788964-60788983 Msa0779540:CDS 25.0%
! AATATTCTCCAAGTTTGATA+TGG - chr5_2:60789049-60789068 Msa0779540:CDS 25.0%
! TATAATCACCATATCAAACT+TGG + chr5_2:60789060-60789079 None:intergenic 25.0%
! AATATAACTAGGAATATGAG+TGG - chr5_2:60789608-60789627 Msa0779540:intron 25.0%
!!! ATGTGAATGAATGATTTGTT+TGG - chr5_2:60789693-60789712 Msa0779540:intron 25.0%
! CAGATTAAAACCAAATCAAA+AGG + chr5_2:60789752-60789771 None:intergenic 25.0%
! ACAAAACAACACCAAAATTA+AGG + chr5_2:60789809-60789828 None:intergenic 25.0%
! AATATACTCCAAGTTTGATA+TGG - chr5_2:60788964-60788983 Msa0779540:CDS 25.0%
! AATATTCTCCAAGTTTGATA+TGG - chr5_2:60789049-60789068 Msa0779540:CDS 25.0%
! TATAATCACCATATCAAACT+TGG + chr5_2:60789060-60789079 None:intergenic 25.0%
! AATATAACTAGGAATATGAG+TGG - chr5_2:60789608-60789627 Msa0779540:intron 25.0%
!!! ATGTGAATGAATGATTTGTT+TGG - chr5_2:60789693-60789712 Msa0779540:intron 25.0%
! CAGATTAAAACCAAATCAAA+AGG + chr5_2:60789752-60789771 None:intergenic 25.0%
! ACAAAACAACACCAAAATTA+AGG + chr5_2:60789809-60789828 None:intergenic 25.0%
TAGAATCACCATATCAAACT+TGG + chr5_2:60788975-60788994 None:intergenic 30.0%
! TAATCATGGTTTTCGACAAT+AGG - chr5_2:60789125-60789144 Msa0779540:exon 30.0%
! AGTTTGAGTTTGTAACCTTA+AGG - chr5_2:60789157-60789176 Msa0779540:intron 30.0%
AATTCAGACATAGTTCCTTA+AGG + chr5_2:60789175-60789194 None:intergenic 30.0%
AACTATGTCTGAATTCCATA+TGG - chr5_2:60789180-60789199 Msa0779540:intron 30.0%
! AATCACAAAGAAAGTGTACA+TGG - chr5_2:60789847-60789866 Msa0779540:intron 30.0%
! CAAAAGAGTATGTGCATATT+AGG - chr5_2:60789936-60789955 Msa0779540:intron 30.0%
TGCATATTAGGATACAACAA+AGG - chr5_2:60789948-60789967 Msa0779540:intron 30.0%
ATTAGGATACAACAAAGGAA+TGG - chr5_2:60789953-60789972 Msa0779540:intron 30.0%
ACTCTTTCTTGAGATCTTTA+AGG + chr5_2:60790036-60790055 None:intergenic 30.0%
!!! AAGAAAGAGTTTTGTTGCAA+TGG - chr5_2:60790046-60790065 Msa0779540:intron 30.0%
TAGAATCACCATATCAAACT+TGG + chr5_2:60788975-60788994 None:intergenic 30.0%
! TAATCATGGTTTTCGACAAT+AGG - chr5_2:60789125-60789144 Msa0779540:exon 30.0%
! AGTTTGAGTTTGTAACCTTA+AGG - chr5_2:60789157-60789176 Msa0779540:intron 30.0%
AATTCAGACATAGTTCCTTA+AGG + chr5_2:60789175-60789194 None:intergenic 30.0%
AACTATGTCTGAATTCCATA+TGG - chr5_2:60789180-60789199 Msa0779540:intron 30.0%
! AATCACAAAGAAAGTGTACA+TGG - chr5_2:60789847-60789866 Msa0779540:intron 30.0%
! CAAAAGAGTATGTGCATATT+AGG - chr5_2:60789936-60789955 Msa0779540:intron 30.0%
TGCATATTAGGATACAACAA+AGG - chr5_2:60789948-60789967 Msa0779540:intron 30.0%
ATTAGGATACAACAAAGGAA+TGG - chr5_2:60789953-60789972 Msa0779540:intron 30.0%
ACTCTTTCTTGAGATCTTTA+AGG + chr5_2:60790036-60790055 None:intergenic 30.0%
!!! AAGAAAGAGTTTTGTTGCAA+TGG - chr5_2:60790046-60790065 Msa0779540:intron 30.0%
AGAAGAGAGTTGAAGAGATT+GGG + chr5_2:60788821-60788840 None:intergenic 35.0%
TAGAAGAGAGTTGAAGAGAT+TGG + chr5_2:60788822-60788841 None:intergenic 35.0%
ATAACCTAGATCCTTGCAAT+CGG - chr5_2:60789463-60789482 Msa0779540:intron 35.0%
!! AACTAAGGAGCCTTTTGATT+TGG - chr5_2:60789739-60789758 Msa0779540:intron 35.0%
CAAATGGATCAAATGTGCTT+GGG + chr5_2:60789886-60789905 None:intergenic 35.0%
TGGAAATGTTGTGAATTGCA+AGG - chr5_2:60790066-60790085 Msa0779540:intron 35.0%
AAGGACAACATCAAGATTCA+TGG - chr5_2:60790169-60790188 Msa0779540:five_prime_UTR 35.0%
AGAAGAGAGTTGAAGAGATT+GGG + chr5_2:60788821-60788840 None:intergenic 35.0%
TAGAAGAGAGTTGAAGAGAT+TGG + chr5_2:60788822-60788841 None:intergenic 35.0%
ATAACCTAGATCCTTGCAAT+CGG - chr5_2:60789463-60789482 Msa0779540:intron 35.0%
!! AACTAAGGAGCCTTTTGATT+TGG - chr5_2:60789739-60789758 Msa0779540:intron 35.0%
CAAATGGATCAAATGTGCTT+GGG + chr5_2:60789886-60789905 None:intergenic 35.0%
TGGAAATGTTGTGAATTGCA+AGG - chr5_2:60790066-60790085 Msa0779540:intron 35.0%
AAGGACAACATCAAGATTCA+TGG - chr5_2:60790169-60790188 Msa0779540:five_prime_UTR 35.0%
AGTTGAAGAGATTGGGAATG+TGG + chr5_2:60788814-60788833 None:intergenic 40.0%
TATCTCTATCTGCAGACTAC+AGG - chr5_2:60788860-60788879 Msa0779540:CDS 40.0%
ATTAAGGTGCATGCATGCAT+GGG + chr5_2:60789793-60789812 None:intergenic 40.0%
AATTAAGGTGCATGCATGCA+TGG + chr5_2:60789794-60789813 None:intergenic 40.0%
! GCATGCATGCACCTTAATTT+TGG - chr5_2:60789795-60789814 Msa0779540:intron 40.0%
GCAAATGGATCAAATGTGCT+TGG + chr5_2:60789887-60789906 None:intergenic 40.0%
! CACATTTGATCCATTTGCTG+AGG - chr5_2:60789889-60789908 Msa0779540:intron 40.0%
GTTGTGAATTGCAAGGATCT+TGG - chr5_2:60790073-60790092 Msa0779540:intron 40.0%
GGAAAGATTATCCAACTCCA+AGG - chr5_2:60790094-60790113 Msa0779540:intron 40.0%
AGTTGAAGAGATTGGGAATG+TGG + chr5_2:60788814-60788833 None:intergenic 40.0%
TATCTCTATCTGCAGACTAC+AGG - chr5_2:60788860-60788879 Msa0779540:CDS 40.0%
ATTAAGGTGCATGCATGCAT+GGG + chr5_2:60789793-60789812 None:intergenic 40.0%
AATTAAGGTGCATGCATGCA+TGG + chr5_2:60789794-60789813 None:intergenic 40.0%
! GCATGCATGCACCTTAATTT+TGG - chr5_2:60789795-60789814 Msa0779540:intron 40.0%
GCAAATGGATCAAATGTGCT+TGG + chr5_2:60789887-60789906 None:intergenic 40.0%
! CACATTTGATCCATTTGCTG+AGG - chr5_2:60789889-60789908 Msa0779540:intron 40.0%
GTTGTGAATTGCAAGGATCT+TGG - chr5_2:60790073-60790092 Msa0779540:intron 40.0%
GGAAAGATTATCCAACTCCA+AGG - chr5_2:60790094-60790113 Msa0779540:intron 40.0%
TGAAGAGATTGGGAATGTGG+AGG + chr5_2:60788811-60788830 None:intergenic 45.0%
TCTTCCGATTGCAAGGATCT+AGG + chr5_2:60789470-60789489 None:intergenic 45.0%
GTGTACTTCTTCCGATTGCA+AGG + chr5_2:60789477-60789496 None:intergenic 45.0%
GATTCTTTGGCCTCAGCAAA+TGG + chr5_2:60789902-60789921 None:intergenic 45.0%
ACCTGGTGCATCTGATTCTT+TGG + chr5_2:60789915-60789934 None:intergenic 45.0%
! TGCACATACTCTTTTGCACC+TGG + chr5_2:60789932-60789951 None:intergenic 45.0%
AAGAGCCTAACAACAGTGCA+AGG - chr5_2:60789980-60789999 Msa0779540:intron 45.0%
TCAGTCCTTGCACTGTTGTT+AGG + chr5_2:60789988-60790007 None:intergenic 45.0%
! CATTCTTGCGTTGATCACCT+TGG + chr5_2:60790114-60790133 None:intergenic 45.0%
TCTCACTTCCTCATTCATGC+TGG - chr5_2:60790136-60790155 Msa0779540:five_prime_UTR 45.0%
TAGCAAGACCAGCATGAATG+AGG + chr5_2:60790147-60790166 None:intergenic 45.0%
!! TCATGCTGGTCTTGCTAGAA+AGG - chr5_2:60790150-60790169 Msa0779540:five_prime_UTR 45.0%
TGAAGAGATTGGGAATGTGG+AGG + chr5_2:60788811-60788830 None:intergenic 45.0%
TCTTCCGATTGCAAGGATCT+AGG + chr5_2:60789470-60789489 None:intergenic 45.0%
GTGTACTTCTTCCGATTGCA+AGG + chr5_2:60789477-60789496 None:intergenic 45.0%
GATTCTTTGGCCTCAGCAAA+TGG + chr5_2:60789902-60789921 None:intergenic 45.0%
ACCTGGTGCATCTGATTCTT+TGG + chr5_2:60789915-60789934 None:intergenic 45.0%
! TGCACATACTCTTTTGCACC+TGG + chr5_2:60789932-60789951 None:intergenic 45.0%
AAGAGCCTAACAACAGTGCA+AGG - chr5_2:60789980-60789999 Msa0779540:intron 45.0%
TCAGTCCTTGCACTGTTGTT+AGG + chr5_2:60789988-60790007 None:intergenic 45.0%
! CATTCTTGCGTTGATCACCT+TGG + chr5_2:60790114-60790133 None:intergenic 45.0%
TCTCACTTCCTCATTCATGC+TGG - chr5_2:60790136-60790155 Msa0779540:five_prime_UTR 45.0%
TAGCAAGACCAGCATGAATG+AGG + chr5_2:60790147-60790166 None:intergenic 45.0%
!! TCATGCTGGTCTTGCTAGAA+AGG - chr5_2:60790150-60790169 Msa0779540:five_prime_UTR 45.0%
!!! GTTATATTTTAAAAATAATT+TGG + chr5_2:60789562-60789581 None:intergenic 5.0%
!!! ATTATTTTTAAAATATAACT+AGG - chr5_2:60789563-60789582 Msa0779540:intron 5.0%
!!! GTTATATTTTAAAAATAATT+TGG + chr5_2:60789562-60789581 None:intergenic 5.0%
!!! ATTATTTTTAAAATATAACT+AGG - chr5_2:60789563-60789582 Msa0779540:intron 5.0%
!!! GTTATATTTTAAAAATAATT+TGG + chr5_2:60789562-60789581 None:intergenic 5.0%
!!! ATTATTTTTAAAATATAACT+AGG - chr5_2:60789563-60789582 Msa0779540:intron 5.0%
!!! GTTATATTTTAAAAATAATT+TGG + chr5_2:60789562-60789581 None:intergenic 5.0%
!!! ATTATTTTTAAAATATAACT+AGG - chr5_2:60789563-60789582 Msa0779540:intron 5.0%
GAGATTGGGAATGTGGAGGT+GGG + chr5_2:60788807-60788826 None:intergenic 50.0%
AGAGATTGGGAATGTGGAGG+TGG + chr5_2:60788808-60788827 None:intergenic 50.0%
GCCAAAGAATCAGATGCACC+AGG - chr5_2:60789911-60789930 Msa0779540:intron 50.0%
! TGCGTTGATCACCTTGGAGT+TGG + chr5_2:60790108-60790127 None:intergenic 50.0%
GAGATTGGGAATGTGGAGGT+GGG + chr5_2:60788807-60788826 None:intergenic 50.0%
AGAGATTGGGAATGTGGAGG+TGG + chr5_2:60788808-60788827 None:intergenic 50.0%
GCCAAAGAATCAGATGCACC+AGG - chr5_2:60789911-60789930 Msa0779540:intron 50.0%
! TGCGTTGATCACCTTGGAGT+TGG + chr5_2:60790108-60790127 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
chr5_2 gene 60788804 60790198 60788804 ID=Msa0779540;Name=Msa0779540
chr5_2 mRNA 60788804 60790198 60788804 ID=Msa0779540-mRNA-1;Parent=Msa0779540;Name=Msa0779540-mRNA-1;_AED=0.15;_eAED=0.21;_QI=101|0|0.5|1|0|0|2|0|110
chr5_2 exon 60788804 60789159 60788804 ID=Msa0779540-mRNA-1:exon:17903;Parent=Msa0779540-mRNA-1
chr5_2 exon 60790121 60790198 60790121 ID=Msa0779540-mRNA-1:exon:17902;Parent=Msa0779540-mRNA-1
chr5_2 five_prime_UTR 60790121 60790198 60790121 ID=Msa0779540-mRNA-1:five_prime_utr;Parent=Msa0779540-mRNA-1
chr5_2 five_prime_UTR 60789137 60789159 60789137 ID=Msa0779540-mRNA-1:five_prime_utr;Parent=Msa0779540-mRNA-1
chr5_2 CDS 60788804 60789136 60788804 ID=Msa0779540-mRNA-1:cds;Parent=Msa0779540-mRNA-1
Gene Sequence

>Msa0779540

ATGGTTGATATTCAGATCCCAAGCACATTTGATCCATTTGCTGAGGCCAAAGAATCAGATGCACCAGGTGCAAAAGAGTATGTGCATATTAGGATACAACAAAGGAATGGAAAAAAGAGCCTAACAACAGTGCAAGGACTGAAAAAAGAGTTCAGCTATGAGAAGATCCTTAAAGATCTCAAGAAAGAGTTTTGTTGCAATGGAAATGTTGTGAATTGCAAGGATCTTGGAAAGATTATCCAACTCCAAGGTGATCAACGCAAGAATGTTTCTCACTTCCTCATTCATGCTGGTCTTGCTAGAAAGGACAACATCAAGATTCATGGTTTTTAA

Protein sequence

>Msa0779540

MVDIQIPSTFDPFAEAKESDAPGAKEYVHIRIQQRNGKKSLTTVQGLKKEFSYEKILKDLKKEFCCNGNVVNCKDLGKIIQLQGDQRKNVSHFLIHAGLARKDNIKIHGF*