Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0825330 | KEH28467.1 | 85.000 | 100 | 10 | 1 | 1 | 100 | 58 | 152 | 3.55e-29 | 112 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0825330 | A0A072UFK4 | 85.000 | 100 | 10 | 1 | 1 | 100 | 58 | 152 | 1.69e-29 | 112 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0017510 | Msa0825330 | 0.807047 | 6.117691e-50 | -8.615850e-47 |
| Msa0045790 | Msa0825330 | 0.807330 | 5.327289e-50 | -8.615850e-47 |
| Msa0046160 | Msa0825330 | 0.803661 | 3.133636e-49 | -8.615850e-47 |
| Msa0657970 | Msa0825330 | 0.803578 | 3.260958e-49 | -8.615850e-47 |
| Msa0693380 | Msa0825330 | 0.803172 | 3.957957e-49 | -8.615850e-47 |
| Msa0724210 | Msa0825330 | 0.800764 | 1.236920e-48 | -8.615850e-47 |
| Msa0744040 | Msa0825330 | 0.885144 | 1.046918e-71 | -8.615850e-47 |
| Msa0760130 | Msa0825330 | 0.800450 | 1.432910e-48 | -8.615850e-47 |
| Msa0810220 | Msa0825330 | 0.808559 | 2.917945e-50 | -8.615850e-47 |
| Msa0825330 | Msa0825340 | 0.810797 | 9.641361e-51 | -8.615850e-47 |
| Msa0825330 | Msa0880140 | 0.801711 | 7.916338e-49 | -8.615850e-47 |
| Msa0825330 | Msa0880950 | 0.809729 | 1.638583e-50 | -8.615850e-47 |
| Msa0825330 | Msa0926890 | 0.823032 | 1.723368e-53 | -8.615850e-47 |
| Msa0825330 | Msa0938220 | 0.808682 | 2.747445e-50 | -8.615850e-47 |
| Msa0825330 | Msa0969520 | 0.805144 | 1.537990e-49 | -8.615850e-47 |
| Msa0825330 | Msa0975220 | 0.800855 | 1.184659e-48 | -8.615850e-47 |
| Msa0825330 | Msa1014540 | 0.802795 | 4.735080e-49 | -8.615850e-47 |
| Msa0825330 | Msa1030970 | 0.803126 | 4.045659e-49 | -8.615850e-47 |
| Msa0825330 | Msa1035300 | 0.802378 | 5.772414e-49 | -8.615850e-47 |
| Msa0825330 | Msa1045200 | 0.819205 | 1.312723e-52 | -8.615850e-47 |
| Msa0825330 | Msa1082500 | 0.816217 | 6.197407e-52 | -8.615850e-47 |
| Msa0825330 | Msa1085300 | 0.802558 | 5.299208e-49 | -8.615850e-47 |
| Msa0825330 | Msa1238930 | 0.812671 | 3.769746e-51 | -8.615850e-47 |
| Msa0825330 | Msa1270610 | 0.801476 | 8.845318e-49 | -8.615850e-47 |
| Msa0825330 | Msa1283860 | 0.801658 | 8.116741e-49 | -8.615850e-47 |
| Msa0825330 | Msa1306840 | 0.801585 | 8.400136e-49 | -8.615850e-47 |
| Msa0825330 | Msa1327050 | 0.800552 | 1.365860e-48 | -8.615850e-47 |
| Msa0825330 | Msa1409700 | 0.811524 | 6.707136e-51 | -8.615850e-47 |
| Msa0825330 | Msa1429900 | 0.807480 | 4.952973e-50 | -8.615850e-47 |
| Msa0825330 | Msa1436480 | 0.801884 | 7.293355e-49 | -8.615850e-47 |
| Msa0825330 | Msa1460020 | 0.827531 | 1.485797e-54 | -8.615850e-47 |
| Msa0157290 | Msa0825330 | 0.808654 | 2.784613e-50 | -8.615850e-47 |
| Msa0212990 | Msa0825330 | 0.805375 | 1.376078e-49 | -8.615850e-47 |
| Msa0376350 | Msa0825330 | 0.800054 | 1.725403e-48 | -8.615850e-47 |
| Msa0414230 | Msa0825330 | 0.801326 | 9.492702e-49 | -8.615850e-47 |
| Msa0452710 | Msa0825330 | 0.802291 | 6.016386e-49 | -8.615850e-47 |
| Msa0345970 | Msa0825330 | 0.805117 | 1.558328e-49 | -8.615850e-47 |
| Msa0484780 | Msa0825330 | 0.814787 | 1.289853e-51 | -8.615850e-47 |
| Msa0546680 | Msa0825330 | 0.809778 | 1.599272e-50 | -8.615850e-47 |
| Msa0563840 | Msa0825330 | 0.802690 | 4.978406e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0825330 | MtrunA17_Chr5g0446481 | 57.143 | 105 | 24 | 3 | 6 | 94 | 8 | 107 | 1.51e-21 | 87.4 |
| Msa0825330 | MtrunA17_Chr5g0447051 | 79.592 | 49 | 10 | 0 | 1 | 49 | 24 | 72 | 5.71e-20 | 76.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 24 sgRNAs with CRISPR-Local
Find 37 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCTTCCCAACCACCAATTT+TGG | 0.163736 | 5_3:-65106909 | None:intergenic |
| ACAGATAACGATACCAAAAT+TGG | 0.306382 | 5_3:+65106896 | Msa0825330:CDS |
| GGATAACGAAGTCAAAGATT+TGG | 0.387444 | 5_3:+65106773 | Msa0825330:CDS |
| CGATACCAAAATTGGTGGTT+GGG | 0.404154 | 5_3:+65106904 | Msa0825330:CDS |
| GAGGATAAGAAAGTTGATGA+AGG | 0.462866 | 5_3:+65106938 | Msa0825330:CDS |
| ATTGCCATTTGAGTCAGTCT+TGG | 0.463769 | 5_3:-65106676 | None:intergenic |
| AGACTGACTCAAATGGCAAT+AGG | 0.467994 | 5_3:+65106679 | Msa0825330:CDS |
| ACCAAAATTGGTGGTTGGGA+AGG | 0.477978 | 5_3:+65106908 | Msa0825330:CDS |
| ACGATACCAAAATTGGTGGT+TGG | 0.479374 | 5_3:+65106903 | Msa0825330:CDS |
| TGGGGTGGAAGAACTAGAAA+TGG | 0.500417 | 5_3:+65106838 | Msa0825330:intron |
| AAAGAGGATGAGAAAGATGT+TGG | 0.522340 | 5_3:+65106971 | Msa0825330:CDS |
| GTTTCCAAGACTGACTCAAA+TGG | 0.535048 | 5_3:+65106672 | Msa0825330:CDS |
| AGATGACATGGGTGAAAAGA+AGG | 0.535573 | 5_3:+65106719 | Msa0825330:CDS |
| AGGATAAGAAAGTTGATGAA+GGG | 0.547120 | 5_3:+65106939 | Msa0825330:CDS |
| TGAAGGGAAAGAAACAAAAG+AGG | 0.565665 | 5_3:+65106955 | Msa0825330:CDS |
| TAACGAAGTCAAAGATTTGG+AGG | 0.567042 | 5_3:+65106776 | Msa0825330:CDS |
| GAAGTCAAAGATTTGGAGGA+TGG | 0.574033 | 5_3:+65106780 | Msa0825330:CDS |
| AAGGACCTGGAACCTAAAGT+AGG | 0.578565 | 5_3:+65106738 | Msa0825330:CDS |
| GATAACGATACCAAAATTGG+TGG | 0.582971 | 5_3:+65106899 | Msa0825330:CDS |
| AGAAAACCACCTAGATGACA+TGG | 0.586850 | 5_3:+65106707 | Msa0825330:CDS |
| CATGGGTGAAAAGAAGGACC+TGG | 0.596256 | 5_3:+65106725 | Msa0825330:CDS |
| TTTCACCCATGTCATCTAGG+TGG | 0.597167 | 5_3:-65106713 | None:intergenic |
| TGGTTGGGAAGGAATAAAAG+AGG | 0.648604 | 5_3:+65106919 | Msa0825330:CDS |
| GAAAACCACCTAGATGACAT+GGG | 0.708635 | 5_3:+65106708 | Msa0825330:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AGTTGATAAAAAAAAACTGA+TGG | + | chr5_3:65106818-65106837 | Msa0825330:intron | 20.0% |
| !!! | GTTGATAAAAAAAAACTGAT+GGG | + | chr5_3:65106819-65106838 | Msa0825330:intron | 20.0% |
| !! | TTGATAAAAAAAAACTGATG+GGG | + | chr5_3:65106820-65106839 | Msa0825330:intron | 20.0% |
| !!! | CTACATTGGTTTCATTAAAT+TGG | - | chr5_3:65106637-65106656 | None:intergenic | 25.0% |
| ATATTATCTGAGCACTACAT+TGG | - | chr5_3:65106651-65106670 | None:intergenic | 30.0% | |
| ATAAAAAAAAACTGATGGGG+TGG | + | chr5_3:65106823-65106842 | Msa0825330:intron | 30.0% | |
| GATGATAAAAAAGCTGATGA+TGG | + | chr5_3:65106863-65106882 | Msa0825330:CDS | 30.0% | |
| ACAGATAACGATACCAAAAT+TGG | + | chr5_3:65106896-65106915 | Msa0825330:CDS | 30.0% | |
| !! | AGGATAAGAAAGTTGATGAA+GGG | + | chr5_3:65106939-65106958 | Msa0825330:CDS | 30.0% |
| !! | TTTTTCCTACTTTAGGTTCC+AGG | - | chr5_3:65106746-65106765 | None:intergenic | 35.0% |
| ACCTAAAGTAGGAAAAATCG+TGG | + | chr5_3:65106749-65106768 | Msa0825330:CDS | 35.0% | |
| ! | TCCACGATTTTTCCTACTTT+AGG | - | chr5_3:65106753-65106772 | None:intergenic | 35.0% |
| TAAAGTAGGAAAAATCGTGG+AGG | + | chr5_3:65106752-65106771 | Msa0825330:CDS | 35.0% | |
| GGATAACGAAGTCAAAGATT+TGG | + | chr5_3:65106773-65106792 | Msa0825330:CDS | 35.0% | |
| TAACGAAGTCAAAGATTTGG+AGG | + | chr5_3:65106776-65106795 | Msa0825330:CDS | 35.0% | |
| GATAACGATACCAAAATTGG+TGG | + | chr5_3:65106899-65106918 | Msa0825330:CDS | 35.0% | |
| !! | GAGGATAAGAAAGTTGATGA+AGG | + | chr5_3:65106938-65106957 | Msa0825330:CDS | 35.0% |
| TGAAGGGAAAGAAACAAAAG+AGG | + | chr5_3:65106955-65106974 | Msa0825330:CDS | 35.0% | |
| AAAGAGGATGAGAAAGATGT+TGG | + | chr5_3:65106971-65106990 | Msa0825330:CDS | 35.0% | |
| ! | GTTTCCAAGACTGACTCAAA+TGG | + | chr5_3:65106672-65106691 | Msa0825330:CDS | 40.0% |
| ! | ATTGCCATTTGAGTCAGTCT+TGG | - | chr5_3:65106679-65106698 | None:intergenic | 40.0% |
| ! | AGACTGACTCAAATGGCAAT+AGG | + | chr5_3:65106679-65106698 | Msa0825330:CDS | 40.0% |
| AGAAAACCACCTAGATGACA+TGG | + | chr5_3:65106707-65106726 | Msa0825330:CDS | 40.0% | |
| GAAAACCACCTAGATGACAT+GGG | + | chr5_3:65106708-65106727 | Msa0825330:CDS | 40.0% | |
| ! | TCTTTTCACCCATGTCATCT+AGG | - | chr5_3:65106719-65106738 | None:intergenic | 40.0% |
| AGATGACATGGGTGAAAAGA+AGG | + | chr5_3:65106719-65106738 | Msa0825330:CDS | 40.0% | |
| GAAGTCAAAGATTTGGAGGA+TGG | + | chr5_3:65106780-65106799 | Msa0825330:CDS | 40.0% | |
| ! | ACGATACCAAAATTGGTGGT+TGG | + | chr5_3:65106903-65106922 | Msa0825330:CDS | 40.0% |
| ! | CGATACCAAAATTGGTGGTT+GGG | + | chr5_3:65106904-65106923 | Msa0825330:CDS | 40.0% |
| TGGTTGGGAAGGAATAAAAG+AGG | + | chr5_3:65106919-65106938 | Msa0825330:CDS | 40.0% | |
| TTTCACCCATGTCATCTAGG+TGG | - | chr5_3:65106716-65106735 | None:intergenic | 45.0% | |
| AAGGACCTGGAACCTAAAGT+AGG | + | chr5_3:65106738-65106757 | Msa0825330:CDS | 45.0% | |
| GGAGGATGGTATGAAAGCAA+TGG | + | chr5_3:65106794-65106813 | Msa0825330:intron | 45.0% | |
| TGGGGTGGAAGAACTAGAAA+TGG | + | chr5_3:65106838-65106857 | Msa0825330:intron | 45.0% | |
| ! | ACCAAAATTGGTGGTTGGGA+AGG | + | chr5_3:65106908-65106927 | Msa0825330:CDS | 45.0% |
| ! | TCCTTCCCAACCACCAATTT+TGG | - | chr5_3:65106912-65106931 | None:intergenic | 45.0% |
| CATGGGTGAAAAGAAGGACC+TGG | + | chr5_3:65106725-65106744 | Msa0825330:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_3 | gene | 65106623 | 65107000 | 65106623 | ID=Msa0825330;Name=Msa0825330 |
| chr5_3 | mRNA | 65106623 | 65107000 | 65106623 | ID=Msa0825330-mRNA-1;Parent=Msa0825330;Name=Msa0825330-mRNA-1;_AED=0.42;_eAED=0.45;_QI=0|0|0|1|0|0|3|0|100 |
| chr5_3 | exon | 65106623 | 65106628 | 65106623 | ID=Msa0825330-mRNA-1:exon:20412;Parent=Msa0825330-mRNA-1 |
| chr5_3 | exon | 65106657 | 65106801 | 65106657 | ID=Msa0825330-mRNA-1:exon:20413;Parent=Msa0825330-mRNA-1 |
| chr5_3 | exon | 65106849 | 65107000 | 65106849 | ID=Msa0825330-mRNA-1:exon:20414;Parent=Msa0825330-mRNA-1 |
| chr5_3 | CDS | 65106623 | 65106628 | 65106623 | ID=Msa0825330-mRNA-1:cds;Parent=Msa0825330-mRNA-1 |
| chr5_3 | CDS | 65106657 | 65106801 | 65106657 | ID=Msa0825330-mRNA-1:cds;Parent=Msa0825330-mRNA-1 |
| chr5_3 | CDS | 65106849 | 65107000 | 65106849 | ID=Msa0825330-mRNA-1:cds;Parent=Msa0825330-mRNA-1 |
| Gene Sequence |
| Protein sequence |
