Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0895270 | MCH91222.1 | 81.633 | 49 | 9 | 0 | 7 | 55 | 23 | 71 | 1.71e-16 | 77.4 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0895270 | A0A392MVA1 | 81.633 | 49 | 9 | 0 | 7 | 55 | 23 | 71 | 8.19e-17 | 77.4 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0726140 | Msa0895270 | 0.809466 | 1.866476e-50 | -8.615850e-47 |
| Msa0743810 | Msa0895270 | 0.816315 | 5.892163e-52 | -8.615850e-47 |
| Msa0745060 | Msa0895270 | 0.836269 | 1.033139e-56 | -8.615850e-47 |
| Msa0783160 | Msa0895270 | 0.812987 | 3.214618e-51 | -8.615850e-47 |
| Msa0825860 | Msa0895270 | 0.836370 | 9.735169e-57 | -8.615850e-47 |
| Msa0406120 | Msa0895270 | 0.804081 | 2.563386e-49 | -8.615850e-47 |
| Msa0414410 | Msa0895270 | 0.825875 | 3.693362e-54 | -8.615850e-47 |
| Msa0414420 | Msa0895270 | 0.832372 | 9.814787e-56 | -8.615850e-47 |
| Msa0452900 | Msa0895270 | 0.841116 | 5.774036e-58 | -8.615850e-47 |
| Msa0267000 | Msa0895270 | 0.811116 | 8.221099e-51 | -8.615850e-47 |
| Msa0267010 | Msa0895270 | 0.819305 | 1.245294e-52 | -8.615850e-47 |
| Msa0342240 | Msa0895270 | 0.804498 | 2.099097e-49 | -8.615850e-47 |
| Msa0491590 | Msa0895270 | 0.801191 | 1.011406e-48 | -8.615850e-47 |
| Msa0502760 | Msa0895270 | 0.807598 | 4.673523e-50 | -8.615850e-47 |
| Msa0564890 | Msa0895270 | 0.816141 | 6.442952e-52 | -8.615850e-47 |
| Msa0865320 | Msa0895270 | 0.817608 | 3.019957e-52 | -8.615850e-47 |
| Msa0895270 | Msa1016280 | 0.838223 | 3.267626e-57 | -8.615850e-47 |
| Msa0895270 | Msa1025360 | 0.828732 | 7.634076e-55 | -8.615850e-47 |
| Msa0895270 | Msa1025370 | 0.821063 | 4.928389e-53 | -8.615850e-47 |
| Msa0895270 | Msa1044830 | 0.825734 | 3.988702e-54 | -8.615850e-47 |
| Msa0895270 | Msa1044840 | 0.828371 | 9.333244e-55 | -8.615850e-47 |
| Msa0895270 | Msa1060840 | 0.806596 | 7.618516e-50 | -8.615850e-47 |
| Msa0895270 | Msa1092990 | 0.811464 | 6.909280e-51 | -8.615850e-47 |
| Msa0895270 | Msa1100980 | 0.816846 | 4.479892e-52 | -8.615850e-47 |
| Msa0895270 | Msa1109440 | 0.844086 | 9.400385e-59 | -8.615850e-47 |
| Msa0895270 | Msa1121200 | 0.801781 | 7.659354e-49 | -8.615850e-47 |
| Msa0895270 | Msa1146110 | 0.814436 | 1.542574e-51 | -8.615850e-47 |
| Msa0895270 | Msa1187940 | 0.813628 | 2.325244e-51 | -8.615850e-47 |
| Msa0895270 | Msa1192640 | 0.805379 | 1.373678e-49 | -8.615850e-47 |
| Msa0895270 | Msa1269670 | 0.834533 | 2.836246e-56 | -8.615850e-47 |
| Msa0895270 | Msa1270230 | 0.829990 | 3.778559e-55 | -8.615850e-47 |
| Msa0895270 | Msa1270270 | 0.818153 | 2.273763e-52 | -8.615850e-47 |
| Msa0895270 | Msa1283330 | 0.816621 | 5.030781e-52 | -8.615850e-47 |
| Msa0895270 | Msa1285930 | 0.814566 | 1.443358e-51 | -8.615850e-47 |
| Msa0895270 | Msa1287800 | 0.823653 | 1.233956e-53 | -8.615850e-47 |
| Msa0895270 | Msa1287810 | 0.835361 | 1.754634e-56 | -8.615850e-47 |
| Msa0895270 | Msa1307890 | 0.807539 | 4.812159e-50 | -8.615850e-47 |
| Msa0895270 | Msa1404000 | 0.829489 | 5.002507e-55 | -8.615850e-47 |
| Msa0895270 | Msa1415310 | 0.805714 | 1.168062e-49 | -8.615850e-47 |
| Msa0895270 | Msa1422180 | 0.812411 | 4.296676e-51 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0895270 | MtrunA17_Chr7g0241491 | 59.756 | 82 | 6 | 1 | 1 | 55 | 271 | 352 | 1.39e-18 | 78.6 |
| Msa0895270 | MtrunA17_Chr7g0241441 | 81.818 | 55 | 10 | 0 | 1 | 55 | 280 | 334 | 3.43e-15 | 68.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 16 sgRNAs with CRISPR-Local
Find 39 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGAGACTTGGAAGACCATTT+AGG | 0.229412 | 6_1:+82744536 | None:intergenic |
| TTATCAGACAATCATGGATT+TGG | 0.280162 | 6_1:-82744631 | Msa0895270:intron |
| AATATTCTTTCATCCCTAAA+TGG | 0.291753 | 6_1:-82744550 | Msa0895270:CDS |
| GAGACTTGGAAGACCATTTA+GGG | 0.317902 | 6_1:+82744537 | None:intergenic |
| TTATACAAATCAACAATTGC+TGG | 0.341452 | 6_1:-82744302 | Msa0895270:CDS |
| TTGAGTTACAACAACTTGAA+TGG | 0.403952 | 6_1:-82744502 | Msa0895270:CDS |
| AAAGAATATTGTTACTCAAA+TGG | 0.406460 | 6_1:+82744563 | None:intergenic |
| CAGCGAAAGAACTTGTAAAC+TGG | 0.419953 | 6_1:+82744594 | None:intergenic |
| TCTTCCAAGTCTCAAATCTC+TGG | 0.457591 | 6_1:-82744527 | Msa0895270:CDS |
| AATGGATCGCTAGATATTAG+TGG | 0.511574 | 6_1:-82744484 | Msa0895270:intron |
| AAGTCCAGAGATTTGAGACT+TGG | 0.521249 | 6_1:+82744523 | None:intergenic |
| ATCATGGATTTGGATTTGCA+TGG | 0.562788 | 6_1:-82744621 | Msa0895270:CDS |
| TCAACAATTGCTGGTGTACG+TGG | 0.590971 | 6_1:-82744293 | Msa0895270:CDS |
| ATTTGTATAAGAAATATCCA+AGG | 0.624404 | 6_1:+82744315 | None:intergenic |
| TTCAGCAATGCTAAGACTCG+AGG | 0.644018 | 6_1:-82744261 | Msa0895270:CDS |
| GAAAGAACTTGTAAACTGGA+CGG | 0.667254 | 6_1:+82744598 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATATAAACATATATGATTAG+AGG | + | chr6_1:82744325-82744344 | None:intergenic | 15.0% |
| !!! | TGAGAACATTTTTTTTTACT+AGG | + | chr6_1:82744468-82744487 | None:intergenic | 20.0% |
| !!! | TTTTTTTTATCAGACAATCA+TGG | - | chr6_1:82744492-82744511 | Msa0895270:CDS | 20.0% |
| !! | AAAGAATATTGTTACTCAAA+TGG | + | chr6_1:82744569-82744588 | None:intergenic | 20.0% |
| !! | ATTTGTATAAGAAATATCCA+AGG | + | chr6_1:82744817-82744836 | None:intergenic | 20.0% |
| !! | GACATATATGAAAGTATGAA+TGG | + | chr6_1:82744266-82744285 | None:intergenic | 25.0% |
| !!! | TTTTCCTTCTGAAAAACATA+TGG | - | chr6_1:82744299-82744318 | Msa0895270:CDS | 25.0% |
| ! | TATATTCAAACTGAAGACTT+TGG | - | chr6_1:82744352-82744371 | Msa0895270:intron | 25.0% |
| ! | ATATTCAAACTGAAGACTTT+GGG | - | chr6_1:82744353-82744372 | Msa0895270:intron | 25.0% |
| ! | TTACTAGGAATATGATATCA+GGG | + | chr6_1:82744453-82744472 | None:intergenic | 25.0% |
| ! | TTTACTAGGAATATGATATC+AGG | + | chr6_1:82744454-82744473 | None:intergenic | 25.0% |
| ! | AATATTCTTTCATCCCTAAA+TGG | - | chr6_1:82744579-82744598 | Msa0895270:CDS | 25.0% |
| ! | ACGATATCTATTTATCTTCT+TGG | - | chr6_1:82744720-82744739 | Msa0895270:intron | 25.0% |
| ! | CGATATCTATTTATCTTCTT+GGG | - | chr6_1:82744721-82744740 | Msa0895270:intron | 25.0% |
| ! | TTATACAAATCAACAATTGC+TGG | - | chr6_1:82744827-82744846 | Msa0895270:intron | 25.0% |
| TATTCAAACTGAAGACTTTG+GGG | - | chr6_1:82744354-82744373 | Msa0895270:intron | 30.0% | |
| TTATCAGACAATCATGGATT+TGG | - | chr6_1:82744498-82744517 | Msa0895270:CDS | 30.0% | |
| TTGAGTTACAACAACTTGAA+TGG | - | chr6_1:82744627-82744646 | Msa0895270:CDS | 30.0% | |
| TATCTATTTATCTTCTTGGG+AGG | - | chr6_1:82744724-82744743 | Msa0895270:intron | 30.0% | |
| TGATTCTGTACAAAAACACA+AGG | + | chr6_1:82744792-82744811 | None:intergenic | 30.0% | |
| ! | TTTTGTACAGAATCAAACCT+TGG | - | chr6_1:82744797-82744816 | Msa0895270:intron | 30.0% |
| AACACGAACTTATGGAATCT+AGG | - | chr6_1:82744398-82744417 | Msa0895270:intron | 35.0% | |
| ATCATGGATTTGGATTTGCA+TGG | - | chr6_1:82744508-82744527 | Msa0895270:CDS | 35.0% | |
| GAAAGAACTTGTAAACTGGA+CGG | + | chr6_1:82744534-82744553 | None:intergenic | 35.0% | |
| AATGGATCGCTAGATATTAG+TGG | - | chr6_1:82744645-82744664 | Msa0895270:intron | 35.0% | |
| !! | GAGGCCATATGTTTTTCAGA+AGG | + | chr6_1:82744306-82744325 | None:intergenic | 40.0% |
| CAGCGAAAGAACTTGTAAAC+TGG | + | chr6_1:82744538-82744557 | None:intergenic | 40.0% | |
| GAGACTTGGAAGACCATTTA+GGG | + | chr6_1:82744595-82744614 | None:intergenic | 40.0% | |
| TGAGACTTGGAAGACCATTT+AGG | + | chr6_1:82744596-82744615 | None:intergenic | 40.0% | |
| TCTTCCAAGTCTCAAATCTC+TGG | - | chr6_1:82744602-82744621 | Msa0895270:CDS | 40.0% | |
| ! | AAGTCCAGAGATTTGAGACT+TGG | + | chr6_1:82744609-82744628 | None:intergenic | 40.0% |
| CATAAACATGTGTACCCCAA+AGG | + | chr6_1:82744758-82744777 | None:intergenic | 40.0% | |
| TGTTGTTCGCAGACAAGTCA+AGG | + | chr6_1:82744382-82744401 | None:intergenic | 45.0% | |
| CTGCGAACAACACGAACTTA+TGG | - | chr6_1:82744390-82744409 | Msa0895270:intron | 45.0% | |
| ! | TTGGGAGGCATATTTGCCTT+TGG | - | chr6_1:82744739-82744758 | Msa0895270:intron | 45.0% |
| ! | TGGGAGGCATATTTGCCTTT+GGG | - | chr6_1:82744740-82744759 | Msa0895270:intron | 45.0% |
| TCAACAATTGCTGGTGTACG+TGG | - | chr6_1:82744836-82744855 | Msa0895270:intron | 45.0% | |
| TTCAGCAATGCTAAGACTCG+AGG | - | chr6_1:82744868-82744887 | Msa0895270:intron | 45.0% | |
| ! | GGGAGGCATATTTGCCTTTG+GGG | - | chr6_1:82744741-82744760 | Msa0895270:intron | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6_1 | gene | 82744244 | 82744907 | 82744244 | ID=Msa0895270;Name=Msa0895270 |
| chr6_1 | mRNA | 82744244 | 82744907 | 82744244 | ID=Msa0895270-mRNA-1;Parent=Msa0895270;Name=Msa0895270-mRNA-1;_AED=0.58;_eAED=0.58;_QI=0|0|0|0.66|0.5|0.66|3|0|88 |
| chr6_1 | exon | 82744904 | 82744907 | 82744904 | ID=Msa0895270-mRNA-1:exon:13975;Parent=Msa0895270-mRNA-1 |
| chr6_1 | exon | 82744485 | 82744646 | 82744485 | ID=Msa0895270-mRNA-1:exon:13974;Parent=Msa0895270-mRNA-1 |
| chr6_1 | exon | 82744244 | 82744344 | 82744244 | ID=Msa0895270-mRNA-1:exon:13973;Parent=Msa0895270-mRNA-1 |
| chr6_1 | CDS | 82744904 | 82744907 | 82744904 | ID=Msa0895270-mRNA-1:cds;Parent=Msa0895270-mRNA-1 |
| chr6_1 | CDS | 82744485 | 82744646 | 82744485 | ID=Msa0895270-mRNA-1:cds;Parent=Msa0895270-mRNA-1 |
| chr6_1 | CDS | 82744244 | 82744344 | 82744244 | ID=Msa0895270-mRNA-1:cds;Parent=Msa0895270-mRNA-1 |
| Gene Sequence |
| Protein sequence |
