AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1078580

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1078580 MtrunA17_Chr7g0263541 98.507 268 4 0 1 268 1 268 0.0 540
Msa1078580 MtrunA17_Chr1g0181801 74.476 286 55 4 1 268 59 344 8.13e-147 414
Msa1078580 MtrunA17_Chr7g0225951 36.299 281 128 6 1 259 1 252 3.62e-44 150
Msa1078580 MtrunA17_Chr2g0305051 34.031 191 105 4 43 230 79 251 5.72e-27 109
Msa1078580 MtrunA17_Chr4g0057771 29.412 204 122 4 38 234 31 219 4.60e-24 97.1
Msa1078580 MtrunA17_Chr4g0017231 30.570 193 113 5 43 232 94 268 1.88e-20 88.6
Msa1078580 MtrunA17_Chr4g0018221 24.107 224 125 3 31 252 49 229 7.71e-16 74.7
Msa1078580 MtrunA17_Chr5g0428971 23.361 244 142 3 11 252 6 206 9.68e-16 74.3
Msa1078580 MtrunA17_Chr8g0337051 23.744 219 121 4 34 249 18 193 2.10e-15 73.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1078580 AT2G37250.1 70.280 286 65 5 1 268 1 284 9.01e-139 392
Msa1078580 AT2G39270.1 71.713 251 70 1 18 268 46 295 7.20e-118 340
Msa1078580 AT3G01820.1 36.087 230 103 6 34 258 60 250 1.83e-37 133
Msa1078580 AT5G35170.1 33.796 216 121 5 18 230 62 258 8.24e-30 117
Msa1078580 AT5G35170.2 33.796 216 121 5 18 230 62 258 9.32e-30 117
Msa1078580 AT5G63400.1 31.731 208 124 4 38 240 35 229 8.41e-27 105
Msa1078580 AT5G50370.1 31.220 205 123 4 38 237 36 227 2.20e-26 103
Msa1078580 AT5G50370.2 31.959 194 114 4 38 226 36 216 7.74e-25 99.4
Msa1078580 AT5G47840.2 31.282 195 110 5 37 228 65 238 1.05e-21 91.7
Msa1078580 AT5G47840.1 30.457 197 116 4 37 230 65 243 1.27e-21 92.0
Msa1078580 AT5G63400.2 34.286 140 85 2 38 172 35 172 4.98e-21 88.2
Msa1078580 AT4G25280.1 24.167 240 137 3 29 266 46 242 1.15e-20 89.0
Msa1078580 AT4G25280.2 24.167 240 137 3 29 266 46 242 1.15e-20 89.0
Msa1078580 AT3G60180.1 26.540 211 110 4 28 236 13 180 4.07e-18 80.9
Msa1078580 AT3G60180.2 26.540 211 110 4 28 236 13 180 4.07e-18 80.9
Msa1078580 AT5G26667.3 25.888 197 101 3 42 236 20 173 3.90e-17 78.2
Msa1078580 AT5G26667.2 25.888 197 101 3 42 236 20 173 3.90e-17 78.2
Msa1078580 AT5G26667.4 25.888 197 101 3 42 236 20 173 3.90e-17 78.2
Msa1078580 AT5G26667.1 25.888 197 101 3 42 236 20 173 4.99e-17 77.8

Find 114 sgRNAs with CRISPR-Local

Find 189 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATCAAACTTATACCCTATT+TGG 0.134719 7_2:-77575490 Msa1078580:three_prime_UTR
TAATTTCATCTGACACTAAT+TGG 0.227977 7_2:+77576535 None:intergenic
ATTCTCACCCTTGATGTTAA+TGG 0.236959 7_2:+77576140 None:intergenic
TTCCATGAATCAGGAAATAT+TGG 0.279986 7_2:-77576280 Msa1078580:intron
GCGTTTAACGAGGCGGGTTA+TGG 0.291921 7_2:+77577740 None:intergenic
TCCACGCGCTAAGGGATAAA+AGG 0.310626 7_2:+77577710 None:intergenic
CTAATGGAGTTTGAGCTTCC+TGG 0.337802 7_2:-77575744 Msa1078580:CDS
AACAACTTTGGCCAAGATTC+CGG 0.353841 7_2:+77575714 None:intergenic
GGACAAGCCAAAGGCGAATC+AGG 0.365095 7_2:-77576480 Msa1078580:CDS
TTGCAAGCAGTGCTTCTTCT+CGG 0.365762 7_2:+77576217 None:intergenic
GAATGAAATACATAATTATA+CGG 0.377797 7_2:+77575628 None:intergenic
ACAACTTTGGCCAAGATTCC+GGG 0.378273 7_2:+77575715 None:intergenic
AATGAAACCTGATTCGCCTT+TGG 0.382130 7_2:+77576473 None:intergenic
GGAAGGTTGGCTTCTCGAAT+TGG 0.384291 7_2:-77575555 Msa1078580:three_prime_UTR
CGCCATGAGCTTTCCTCCAA+TGG 0.396544 7_2:-77577534 Msa1078580:CDS
GAATCAGGTTTCATTCTTGA+TGG 0.402493 7_2:-77576465 Msa1078580:CDS
TTGAACTAATGTTATGTTCA+AGG 0.403442 7_2:-77575296 Msa1078580:three_prime_UTR
AGGGGAGCCATACTAATTCC+AGG 0.407675 7_2:+77576111 None:intergenic
TTCTTAGGCTGTCCCGGCGT+TGG 0.409479 7_2:-77577627 Msa1078580:CDS
AAATGGAACCTGGGAAGAAA+GGG 0.413958 7_2:+77577509 None:intergenic
CGAAGCCTTTCTTTGACTAC+TGG 0.419226 7_2:+77576033 None:intergenic
AAAATGGAACCTGGGAAGAA+AGG 0.419929 7_2:+77577508 None:intergenic
TTCGCCTTTGGCTTGTCCTT+CGG 0.420172 7_2:+77576485 None:intergenic
GCTTCGTATATACAATGAAT+TGG 0.421380 7_2:-77576016 Msa1078580:intron
CTGGAGGGATCCCGGAATCT+TGG 0.426061 7_2:-77575725 Msa1078580:CDS
AGCAATAATGCCAACAGGAT+TGG 0.436526 7_2:-77575355 Msa1078580:three_prime_UTR
AGGAGCAAAGCAATAACAAA+AGG 0.437227 7_2:+77577793 None:intergenic
ATTAACATCAAGGGTGAGAA+TGG 0.441265 7_2:-77576138 Msa1078580:CDS
ACTGCTTGCTTTATTGTTCG+AGG 0.454143 7_2:+77576438 None:intergenic
AGTCAATGAAAAGGAGTCAT+TGG 0.455728 7_2:+77575207 None:intergenic
TTAAGAGCATGCAACAACTT+TGG 0.463437 7_2:+77575702 None:intergenic
TTCGAGAAGCCAACCTTCCC+TGG 0.470689 7_2:+77575559 None:intergenic
TTGGTGAGTAATGTGAAATA+AGG 0.470699 7_2:+77575226 None:intergenic
TACAATGCTCAGGAGGAAGC+AGG 0.474529 7_2:+77576091 None:intergenic
GGCTGTCCCGGCGTTGGTAA+AGG 0.475495 7_2:-77577621 Msa1078580:CDS
CGGTGGCGCGTGAGAATATG+AGG 0.478955 7_2:+77577677 None:intergenic
CCGTAGCCGAGGAAAACTAA+TGG 0.480423 7_2:-77575760 Msa1078580:CDS
AGCCGCCTCTGCAACCTCCT+CGG 0.480794 7_2:-77577588 Msa1078580:CDS
GTTTCAGCAATAATGCCAAC+AGG 0.481394 7_2:-77575360 Msa1078580:three_prime_UTR
GTTGCAGAGGCGGCTGGCGT+AGG 0.481502 7_2:+77577596 None:intergenic
GAAATAGAACCAGGGAAGGT+TGG 0.483113 7_2:-77575568 Msa1078580:three_prime_UTR
ATAACAAAAGGGAGATATGA+TGG 0.486153 7_2:+77577805 None:intergenic
ATGTTCAAGGTATTTACAAA+AGG 0.486727 7_2:-77575283 Msa1078580:three_prime_UTR
TGTTGCCTCCATTAACATCA+AGG 0.487139 7_2:-77576148 Msa1078580:CDS
GGAGGAGAATCCACGCGCTA+AGG 0.487598 7_2:+77577701 None:intergenic
CACCGAGGAGGTTGCAGAGG+CGG 0.490243 7_2:+77577586 None:intergenic
GAGCTTGTTGTCTAAGAGAC+TGG 0.490979 7_2:-77576508 Msa1078580:CDS
ACAAAGTATAGTCAATGAAA+AGG 0.495606 7_2:+77575198 None:intergenic
TGGGTACCCTGGAATTAGTA+TGG 0.499410 7_2:-77576118 Msa1078580:CDS
CAAGATTCCGGGATCCCTCC+AGG 0.501896 7_2:+77575726 None:intergenic
TGAGCTTCCTGGAGGGATCC+CGG 0.505662 7_2:-77575733 Msa1078580:CDS
CTGAACCAGTAGTCAAAGAA+AGG 0.515149 7_2:-77576038 Msa1078580:CDS
GAGGAGGTTGCAGAGGCGGC+TGG 0.516659 7_2:+77577590 None:intergenic
TGTGGTGCGTTTAACGAGGC+GGG 0.525206 7_2:+77577734 None:intergenic
AGCTTGGACATACAATGCTC+AGG 0.536250 7_2:+77576081 None:intergenic
GGTGTTCCTCACATCGCTAC+CGG 0.536592 7_2:-77577567 Msa1078580:CDS
GGCCATTGGAGGAAAGCTCA+TGG 0.538613 7_2:+77577532 None:intergenic
CGCGTGAGAATATGAGGAGG+AGG 0.547324 7_2:+77577683 None:intergenic
GGTGACAGACATTGACTTGG+TGG 0.549406 7_2:-77576253 Msa1078580:CDS
CTCATGGCGAACAAGATCTC+CGG 0.552425 7_2:+77577548 None:intergenic
ACAATGCTCAGGAGGAAGCA+GGG 0.553118 7_2:+77576092 None:intergenic
GGAGCAAAGCAATAACAAAA+GGG 0.559545 7_2:+77577794 None:intergenic
ATGAATCAGGAAATATTGGA+AGG 0.560014 7_2:-77576276 Msa1078580:intron
TTAACATCAAGGGTGAGAAT+GGG 0.568815 7_2:-77576137 Msa1078580:CDS
CTACGGTAAAATCCCTCCAC+AGG 0.569867 7_2:+77575777 None:intergenic
GAATCAGGAAATATTGGAAG+GGG 0.571838 7_2:-77576274 Msa1078580:intron
CGCTAAGGGATAAAAGGATG+TGG 0.573069 7_2:+77577716 None:intergenic
ATCAGAGCGAGTGATGAGCT+TGG 0.574720 7_2:+77576065 None:intergenic
CTATCAGAAATCGTAAATCA+AGG 0.576086 7_2:-77576558 Msa1078580:CDS
AGGCGGGTTATGGCCGCCAT+CGG 0.577532 7_2:+77577750 None:intergenic
GCAGGAAATAGAACCAGGGA+AGG 0.585387 7_2:-77575572 Msa1078580:three_prime_UTR
CTCACCCTTGATGTTAATGG+AGG 0.585838 7_2:+77576143 None:intergenic
ATTGTAGAGTCAACCTGTGG+AGG 0.587568 7_2:-77575790 Msa1078580:intron
TAAACAGCAGAGGACATCAA+TGG 0.590881 7_2:+77575254 None:intergenic
TGAATCAGGAAATATTGGAA+GGG 0.590988 7_2:-77576275 Msa1078580:intron
TAGGTACCTTTACCAACGCC+GGG 0.591127 7_2:+77577615 None:intergenic
CTGGCCGAAGGACAAGCCAA+AGG 0.598805 7_2:-77576489 Msa1078580:CDS
GACTGCAAGTTCTCCTACCG+AGG 0.599285 7_2:+77576190 None:intergenic
TTGTAGAGTCAACCTGTGGA+GGG 0.600517 7_2:-77575789 Msa1078580:intron
GCACTGCTTGCAAAATGCCT+CGG 0.600739 7_2:-77576207 Msa1078580:CDS
CAGAGCTGCGATTCCGATGG+CGG 0.601785 7_2:-77577763 Msa1078580:exon
GTTGCCTCCATTAACATCAA+GGG 0.606046 7_2:-77576147 Msa1078580:CDS
GAACACCGAGGAGGTTGCAG+AGG 0.607957 7_2:+77577583 None:intergenic
GAGGAGAATCCACGCGCTAA+GGG 0.613124 7_2:+77577702 None:intergenic
AAGGGTGAGAATGGGTACCC+TGG 0.615990 7_2:-77576129 Msa1078580:CDS
TGATAACAGGAATATTCAGT+GGG 0.619397 7_2:-77577652 Msa1078580:CDS
GATCAGAGCTGCGATTCCGA+TGG 0.624309 7_2:-77577766 Msa1078580:exon
CAATGCTCAGGAGGAAGCAG+GGG 0.629253 7_2:+77576093 None:intergenic
TAAGGTTAGTTAAACAGCAG+AGG 0.631168 7_2:+77575244 None:intergenic
GTGATAACAGGAATATTCAG+TGG 0.631365 7_2:-77577653 Msa1078580:CDS
AATGGAACCTGGGAAGAAAG+GGG 0.635071 7_2:+77577510 None:intergenic
GGGGAGCCATACTAATTCCA+GGG 0.636100 7_2:+77576112 None:intergenic
ATGGAGTTTGAGCTTCCTGG+AGG 0.638339 7_2:-77575741 Msa1078580:CDS
GTAGGTACCTTTACCAACGC+CGG 0.638576 7_2:+77577614 None:intergenic
AGGATGTGGTGCGTTTAACG+AGG 0.640197 7_2:+77577730 None:intergenic
TGGAGTTTGAGCTTCCTGGA+GGG 0.640406 7_2:-77575740 Msa1078580:CDS
TGCTTGCAAAATGCCTCGGT+AGG 0.644240 7_2:-77576203 Msa1078580:CDS
AGTGATTGTGCCAATCCTGT+TGG 0.647325 7_2:+77575345 None:intergenic
AGAACTTGCAGTCAGTGTGG+AGG 0.647767 7_2:-77576180 Msa1078580:CDS
TTGTCTAAGAGACTGGCCGA+AGG 0.649173 7_2:-77576501 Msa1078580:CDS
ATGGAACCTGGGAAGAAAGG+GGG 0.650436 7_2:+77577511 None:intergenic
ATGTGGTGCGTTTAACGAGG+CGG 0.651506 7_2:+77577733 None:intergenic
AGATCTCCGGTAGCGATGTG+AGG 0.653463 7_2:+77577561 None:intergenic
TTGGACATACAATGCTCAGG+AGG 0.663374 7_2:+77576084 None:intergenic
ATATTCCTGTTATCACGCGG+TGG 0.668275 7_2:+77577660 None:intergenic
AGGGGTGACAGACATTGACT+TGG 0.668576 7_2:-77576256 Msa1078580:CDS
AGGAGAACTTGCAGTCAGTG+TGG 0.669907 7_2:-77576183 Msa1078580:CDS
ATGATTGTAGAGTCAACCTG+TGG 0.682094 7_2:-77575793 Msa1078580:intron
AATCGCAGCTCTGATCTCAG+AGG 0.695539 7_2:+77577773 None:intergenic
ACAAAAGGGAGATATGATGG+AGG 0.697516 7_2:+77577808 None:intergenic
TGAATATTCCTGTTATCACG+CGG 0.715299 7_2:+77577657 None:intergenic
CGATGTGAGGAACACCGAGG+AGG 0.716688 7_2:+77577574 None:intergenic
TGGCGCGTGAGAATATGAGG+AGG 0.729208 7_2:+77577680 None:intergenic
TAGCGATGTGAGGAACACCG+AGG 0.819027 7_2:+77577571 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAAAAAGGAAAAATAAAA+TGG + chr7_2:77575499-77575518 None:intergenic 10.0%
!!! TTTTCATTTTGAATAAAAAA+AGG + chr7_2:77575512-77575531 None:intergenic 10.0%
!!! AATAATATTAAAACTTTTAG+TGG + chr7_2:77576212-77576231 None:intergenic 10.0%
!!! TTTTGATAAATAATGTTATA+AGG - chr7_2:77577461-77577480 Msa1078580:intron 10.0%
!! ATTAACAATCATATTGTTTA+AGG + chr7_2:77576087-77576106 None:intergenic 15.0%
!!! GGACTTTTTATATTAATAAT+AGG - chr7_2:77576627-77576646 Msa1078580:intron 15.0%
!! GAATGAAATACATAATTATA+CGG + chr7_2:77577363-77577382 None:intergenic 15.0%
!! ACAAAAAAAAAATCCAAATA+GGG + chr7_2:77577514-77577533 None:intergenic 15.0%
!! AACAAAAAAAAAATCCAAAT+AGG + chr7_2:77577515-77577534 None:intergenic 15.0%
!!! ATACTAGTTTTTTTTAATGT+TGG - chr7_2:77577561-77577580 Msa1078580:CDS 15.0%
!! CCAAAGCATATAATAATAAA+AGG + chr7_2:77575548-77575567 None:intergenic 20.0%
!!! CCTTTTATTATTATATGCTT+TGG - chr7_2:77575545-77575564 Msa1078580:three_prime_UTR 20.0%
!!! TTTTTGATAATACATAGCAA+TGG - chr7_2:77576245-77576264 Msa1078580:CDS 20.0%
!! ATAAAAAGTCCTTAAAAACT+AGG + chr7_2:77576618-77576637 None:intergenic 20.0%
!! ATTATATGCTTTGGTTTAGT+TGG - chr7_2:77575554-77575573 Msa1078580:three_prime_UTR 25.0%
!!! TGATATTGATAGCGTAAAAT+GGG - chr7_2:77575593-77575612 Msa1078580:three_prime_UTR 25.0%
! TTTATGTGTCTTCAATACTT+TGG - chr7_2:77575748-77575767 Msa1078580:CDS 25.0%
! CATGTAATAAGCTGTAATTA+TGG - chr7_2:77575831-77575850 Msa1078580:intron 25.0%
! TTAACAATCAGAATTGTTGA+AGG + chr7_2:77575947-77575966 None:intergenic 25.0%
! AAATGATGATTGACATTCTA+TGG - chr7_2:77575965-77575984 Msa1078580:intron 25.0%
! TAATTTCATCTGACACTAAT+TGG + chr7_2:77576456-77576475 None:intergenic 25.0%
!! TGTATTATTTTCCATGAATC+AGG - chr7_2:77576699-77576718 Msa1078580:intron 25.0%
!! TTGTTATTGTTGTTGGTTAA+GGG - chr7_2:77577023-77577042 Msa1078580:intron 25.0%
! TTGAACTAATGTTATGTTCA+AGG - chr7_2:77577692-77577711 Msa1078580:CDS 25.0%
!! ATGTTCAAGGTATTTACAAA+AGG - chr7_2:77577705-77577724 Msa1078580:CDS 25.0%
! ACAAAGTATAGTCAATGAAA+AGG + chr7_2:77577793-77577812 None:intergenic 25.0%
ATAACAAAAGGGAGATATGA+TGG + chr7_2:77575186-77575205 None:intergenic 30.0%
TGATAACAGGAATATTCAGT+GGG - chr7_2:77575336-77575355 Msa1078580:three_prime_UTR 30.0%
!! AATATTCAGTGGGTTTTCTT+AGG - chr7_2:77575346-77575365 Msa1078580:three_prime_UTR 30.0%
GGAAAAATAAAATGGAACCT+GGG + chr7_2:77575491-77575510 None:intergenic 30.0%
AGGAAAAATAAAATGGAACC+TGG + chr7_2:77575492-77575511 None:intergenic 30.0%
!! CTGATATTGATAGCGTAAAA+TGG - chr7_2:77575592-77575611 Msa1078580:three_prime_UTR 30.0%
!!! AAAATTTGTGCTTTTTCGAC+TGG - chr7_2:77575713-77575732 Msa1078580:CDS 30.0%
! TTGGACTCATTTGTTAAATC+TGG - chr7_2:77575767-77575786 Msa1078580:CDS 30.0%
AATCTCTGATTCATGTTTCA+TGG - chr7_2:77575895-77575914 Msa1078580:intron 30.0%
! ATTGAAGATAGCATGGTTTT+TGG - chr7_2:77576324-77576343 Msa1078580:intron 30.0%
CTATCAGAAATCGTAAATCA+AGG - chr7_2:77576430-77576449 Msa1078580:intron 30.0%
TTCCATGAATCAGGAAATAT+TGG - chr7_2:77576708-77576727 Msa1078580:intron 30.0%
TTCCAATATTTCCTGATTCA+TGG + chr7_2:77576713-77576732 None:intergenic 30.0%
ATGAATCAGGAAATATTGGA+AGG - chr7_2:77576712-77576731 Msa1078580:intron 30.0%
TGAATCAGGAAATATTGGAA+GGG - chr7_2:77576713-77576732 Msa1078580:intron 30.0%
GCTTCGTATATACAATGAAT+TGG - chr7_2:77576972-77576991 Msa1078580:intron 30.0%
! ACTGTTGTTGTTATTGTTGT+TGG - chr7_2:77577016-77577035 Msa1078580:intron 30.0%
! GTTGTTATTGTTGTTGGTTA+AGG - chr7_2:77577022-77577041 Msa1078580:intron 30.0%
! TGTTATTGTTGTTGGTTAAG+GGG - chr7_2:77577024-77577043 Msa1078580:intron 30.0%
AACCTATGGCATCTTAATTA+TGG - chr7_2:77577052-77577071 Msa1078580:intron 30.0%
ACCTATGGCATCTTAATTAT+GGG - chr7_2:77577053-77577072 Msa1078580:intron 30.0%
GATCAAACTTATACCCTATT+TGG - chr7_2:77577498-77577517 Msa1078580:intron 30.0%
TTGGTGAGTAATGTGAAATA+AGG + chr7_2:77577765-77577784 None:intergenic 30.0%
GGAGCAAAGCAATAACAAAA+GGG + chr7_2:77575197-77575216 None:intergenic 35.0%
AGGAGCAAAGCAATAACAAA+AGG + chr7_2:77575198-77575217 None:intergenic 35.0%
TGAATATTCCTGTTATCACG+CGG + chr7_2:77575334-77575353 None:intergenic 35.0%
GTGATAACAGGAATATTCAG+TGG - chr7_2:77575335-77575354 Msa1078580:three_prime_UTR 35.0%
!! GCGTAAAATGGGTTATTCTA+TGG - chr7_2:77575604-77575623 Msa1078580:three_prime_UTR 35.0%
!! TTTTTCGACTGGCTATGTTT+TGG - chr7_2:77575724-77575743 Msa1078580:CDS 35.0%
TCTGGCTATGTAAGAAATCA+TGG - chr7_2:77575785-77575804 Msa1078580:CDS 35.0%
! CTTGTCACTTATCAGTATGT+TGG - chr7_2:77575868-77575887 Msa1078580:intron 35.0%
TTCATGTTTCATGGTTCAAC+TGG - chr7_2:77575904-77575923 Msa1078580:intron 35.0%
TCACACTCACACAAGAATTA+AGG + chr7_2:77576304-77576323 None:intergenic 35.0%
AGTGTGAATTGAAGATAGCA+TGG - chr7_2:77576317-77576336 Msa1078580:intron 35.0%
GAATCAGGTTTCATTCTTGA+TGG - chr7_2:77576523-77576542 Msa1078580:CDS 35.0%
AGTCCTTAAAAACTAGGACA+GGG + chr7_2:77576612-77576631 None:intergenic 35.0%
AAGTCCTTAAAAACTAGGAC+AGG + chr7_2:77576613-77576632 None:intergenic 35.0%
TTCCATCGAAAAATGACACA+AGG - chr7_2:77576671-77576690 Msa1078580:intron 35.0%
!!! TTCCTTGTGTCATTTTTCGA+TGG + chr7_2:77576676-77576695 None:intergenic 35.0%
GAATCAGGAAATATTGGAAG+GGG - chr7_2:77576714-77576733 Msa1078580:intron 35.0%
ATTCTCACCCTTGATGTTAA+TGG + chr7_2:77576851-77576870 None:intergenic 35.0%
ATTAACATCAAGGGTGAGAA+TGG - chr7_2:77576850-77576869 Msa1078580:intron 35.0%
TTAACATCAAGGGTGAGAAT+GGG - chr7_2:77576851-77576870 Msa1078580:intron 35.0%
ACAACAGTAACAGTAAGACA+AGG + chr7_2:77577004-77577023 None:intergenic 35.0%
CCTATGGCATCTTAATTATG+GGG - chr7_2:77577054-77577073 Msa1078580:intron 35.0%
CTATGGCATCTTAATTATGG+GGG - chr7_2:77577055-77577074 Msa1078580:intron 35.0%
!!! CAAACTCCATTAGTTTTCCT+CGG + chr7_2:77577237-77577256 None:intergenic 35.0%
! TTAAGAGCATGCAACAACTT+TGG + chr7_2:77577289-77577308 None:intergenic 35.0%
! TGCATGCTATACAATTTTGC+AGG - chr7_2:77577398-77577417 Msa1078580:intron 35.0%
! ATTTTGCAGGAAATAGAACC+AGG - chr7_2:77577411-77577430 Msa1078580:intron 35.0%
! TTTTGCAGGAAATAGAACCA+GGG - chr7_2:77577412-77577431 Msa1078580:intron 35.0%
! TAAGGTTAGTTAAACAGCAG+AGG + chr7_2:77577747-77577766 None:intergenic 35.0%
AGTCAATGAAAAGGAGTCAT+TGG + chr7_2:77577784-77577803 None:intergenic 35.0%
ACAAAAGGGAGATATGATGG+AGG + chr7_2:77575183-77575202 None:intergenic 40.0%
AAATGGAACCTGGGAAGAAA+GGG + chr7_2:77575482-77575501 None:intergenic 40.0%
AAAATGGAACCTGGGAAGAA+AGG + chr7_2:77575483-77575502 None:intergenic 40.0%
!! TCATGGTTCAACTGGTTTGA+TGG - chr7_2:77575912-77575931 Msa1078580:intron 40.0%
!!! CTTTTGTCACTTGTTGTCGT+TGG - chr7_2:77575993-77576012 Msa1078580:intron 40.0%
CCATCAAAAAGCAGAATGCA+TGG + chr7_2:77576112-77576131 None:intergenic 40.0%
!!! CCATGCATTCTGCTTTTTGA+TGG - chr7_2:77576109-77576128 Msa1078580:CDS 40.0%
! AATGAAACCTGATTCGCCTT+TGG + chr7_2:77576518-77576537 None:intergenic 40.0%
! ACTGCTTGCTTTATTGTTCG+AGG + chr7_2:77576553-77576572 None:intergenic 40.0%
GTCCTTAAAAACTAGGACAG+GGG + chr7_2:77576611-77576630 None:intergenic 40.0%
TGTTGCCTCCATTAACATCA+AGG - chr7_2:77576840-77576859 Msa1078580:intron 40.0%
GTTGCCTCCATTAACATCAA+GGG - chr7_2:77576841-77576860 Msa1078580:intron 40.0%
CTGAACCAGTAGTCAAAGAA+AGG - chr7_2:77576950-77576969 Msa1078580:intron 40.0%
CCCCATAATTAAGATGCCAT+AGG + chr7_2:77577057-77577076 None:intergenic 40.0%
ATGATTGTAGAGTCAACCTG+TGG - chr7_2:77577195-77577214 Msa1078580:intron 40.0%
AACAACTTTGGCCAAGATTC+CGG + chr7_2:77577277-77577296 None:intergenic 40.0%
GTTTCAGCAATAATGCCAAC+AGG - chr7_2:77577628-77577647 Msa1078580:CDS 40.0%
AGCAATAATGCCAACAGGAT+TGG - chr7_2:77577633-77577652 Msa1078580:CDS 40.0%
TAAACAGCAGAGGACATCAA+TGG + chr7_2:77577737-77577756 None:intergenic 40.0%
CGCTAAGGGATAAAAGGATG+TGG + chr7_2:77575275-77575294 None:intergenic 45.0%
ATATTCCTGTTATCACGCGG+TGG + chr7_2:77575331-77575350 None:intergenic 45.0%
AATGGAACCTGGGAAGAAAG+GGG + chr7_2:77575481-77575500 None:intergenic 45.0%
TTGACGCAAATCACGCAGAT+GGG + chr7_2:77575675-77575694 None:intergenic 45.0%
ATTGACGCAAATCACGCAGA+TGG + chr7_2:77575676-77575695 None:intergenic 45.0%
!! GAGCTTGTTGTCTAAGAGAC+TGG - chr7_2:77576480-77576499 Msa1078580:CDS 45.0%
AAAAACTAGGACAGGGGTGA+GGG + chr7_2:77576605-77576624 None:intergenic 45.0%
TAAAAACTAGGACAGGGGTG+AGG + chr7_2:77576606-77576625 None:intergenic 45.0%
!! CACCCCTGTCCTAGTTTTTA+AGG - chr7_2:77576606-77576625 Msa1078580:intron 45.0%
TTGCAAGCAGTGCTTCTTCT+CGG + chr7_2:77576774-77576793 None:intergenic 45.0%
CTCACCCTTGATGTTAATGG+AGG + chr7_2:77576848-77576867 None:intergenic 45.0%
TGGGTACCCTGGAATTAGTA+TGG - chr7_2:77576870-77576889 Msa1078580:intron 45.0%
TTGGACATACAATGCTCAGG+AGG + chr7_2:77576907-77576926 None:intergenic 45.0%
AGCTTGGACATACAATGCTC+AGG + chr7_2:77576910-77576929 None:intergenic 45.0%
CGAAGCCTTTCTTTGACTAC+TGG + chr7_2:77576958-77576977 None:intergenic 45.0%
ATTGTAGAGTCAACCTGTGG+AGG - chr7_2:77577198-77577217 Msa1078580:intron 45.0%
TTGTAGAGTCAACCTGTGGA+GGG - chr7_2:77577199-77577218 Msa1078580:intron 45.0%
!!! CCATTAGTTTTCCTCGGCTA+CGG + chr7_2:77577231-77577250 None:intergenic 45.0%
CTAATGGAGTTTGAGCTTCC+TGG - chr7_2:77577244-77577263 Msa1078580:intron 45.0%
ACAACTTTGGCCAAGATTCC+GGG + chr7_2:77577276-77577295 None:intergenic 45.0%
GAAATAGAACCAGGGAAGGT+TGG - chr7_2:77577420-77577439 Msa1078580:intron 45.0%
AGTGATTGTGCCAATCCTGT+TGG + chr7_2:77577646-77577665 None:intergenic 45.0%
!!! TAATATTATTAATTTTTTTG+AGG - chr7_2:77576222-77576241 Msa1078580:CDS 5.0%
! AATCGCAGCTCTGATCTCAG+AGG + chr7_2:77575218-77575237 None:intergenic 50.0%
ATGTGGTGCGTTTAACGAGG+CGG + chr7_2:77575258-77575277 None:intergenic 50.0%
AGGATGTGGTGCGTTTAACG+AGG + chr7_2:77575261-77575280 None:intergenic 50.0%
! TCCTTTTATCCCTTAGCGCG+TGG - chr7_2:77575277-77575296 Msa1078580:three_prime_UTR 50.0%
TCCACGCGCTAAGGGATAAA+AGG + chr7_2:77575281-77575300 None:intergenic 50.0%
! TGGGTTTTCTTAGGCTGTCC+CGG - chr7_2:77575355-77575374 Msa1078580:three_prime_UTR 50.0%
TAGGTACCTTTACCAACGCC+GGG + chr7_2:77575376-77575395 None:intergenic 50.0%
GTAGGTACCTTTACCAACGC+CGG + chr7_2:77575377-77575396 None:intergenic 50.0%
CTCATGGCGAACAAGATCTC+CGG + chr7_2:77575443-77575462 None:intergenic 50.0%
ATGGAACCTGGGAAGAAAGG+GGG + chr7_2:77575480-77575499 None:intergenic 50.0%
! TTGTCTAAGAGACTGGCCGA+AGG - chr7_2:77576487-77576506 Msa1078580:CDS 50.0%
!! TTCGCCTTTGGCTTGTCCTT+CGG + chr7_2:77576506-77576525 None:intergenic 50.0%
AAAACTAGGACAGGGGTGAG+GGG + chr7_2:77576604-77576623 None:intergenic 50.0%
! AGGGGTGACAGACATTGACT+TGG - chr7_2:77576732-77576751 Msa1078580:intron 50.0%
!! GGTGACAGACATTGACTTGG+TGG - chr7_2:77576735-77576754 Msa1078580:intron 50.0%
GCACTGCTTGCAAAATGCCT+CGG - chr7_2:77576781-77576800 Msa1078580:intron 50.0%
TGCTTGCAAAATGCCTCGGT+AGG - chr7_2:77576785-77576804 Msa1078580:intron 50.0%
AGGAGAACTTGCAGTCAGTG+TGG - chr7_2:77576805-77576824 Msa1078580:intron 50.0%
AGAACTTGCAGTCAGTGTGG+AGG - chr7_2:77576808-77576827 Msa1078580:intron 50.0%
GGGGAGCCATACTAATTCCA+GGG + chr7_2:77576879-77576898 None:intergenic 50.0%
AGGGGAGCCATACTAATTCC+AGG + chr7_2:77576880-77576899 None:intergenic 50.0%
ACAATGCTCAGGAGGAAGCA+GGG + chr7_2:77576899-77576918 None:intergenic 50.0%
TACAATGCTCAGGAGGAAGC+AGG + chr7_2:77576900-77576919 None:intergenic 50.0%
ATCAGAGCGAGTGATGAGCT+TGG + chr7_2:77576926-77576945 None:intergenic 50.0%
GTTAAGGGGACGTGAACCTA+TGG - chr7_2:77577038-77577057 Msa1078580:intron 50.0%
CTACGGTAAAATCCCTCCAC+AGG + chr7_2:77577214-77577233 None:intergenic 50.0%
CCGTAGCCGAGGAAAACTAA+TGG - chr7_2:77577228-77577247 Msa1078580:intron 50.0%
ATGGAGTTTGAGCTTCCTGG+AGG - chr7_2:77577247-77577266 Msa1078580:intron 50.0%
TGGAGTTTGAGCTTCCTGGA+GGG - chr7_2:77577248-77577267 Msa1078580:intron 50.0%
GCAGGAAATAGAACCAGGGA+AGG - chr7_2:77577416-77577435 Msa1078580:intron 50.0%
!! GGAAGGTTGGCTTCTCGAAT+TGG - chr7_2:77577433-77577452 Msa1078580:intron 50.0%
GATCAGAGCTGCGATTCCGA+TGG - chr7_2:77575222-77575241 Msa1078580:three_prime_UTR 55.0%
!! GCGTTTAACGAGGCGGGTTA+TGG + chr7_2:77575251-77575270 None:intergenic 55.0%
TGTGGTGCGTTTAACGAGGC+GGG + chr7_2:77575257-77575276 None:intergenic 55.0%
GAGGAGAATCCACGCGCTAA+GGG + chr7_2:77575289-77575308 None:intergenic 55.0%
CGCGTGAGAATATGAGGAGG+AGG + chr7_2:77575308-77575327 None:intergenic 55.0%
TGGCGCGTGAGAATATGAGG+AGG + chr7_2:77575311-77575330 None:intergenic 55.0%
TAGCGATGTGAGGAACACCG+AGG + chr7_2:77575420-77575439 None:intergenic 55.0%
GGTGTTCCTCACATCGCTAC+CGG - chr7_2:77575421-77575440 Msa1078580:three_prime_UTR 55.0%
AGATCTCCGGTAGCGATGTG+AGG + chr7_2:77575430-77575449 None:intergenic 55.0%
CGCCATGAGCTTTCCTCCAA+TGG - chr7_2:77575454-77575473 Msa1078580:three_prime_UTR 55.0%
GGCCATTGGAGGAAAGCTCA+TGG + chr7_2:77575459-77575478 None:intergenic 55.0%
GGACAAGCCAAAGGCGAATC+AGG - chr7_2:77576508-77576527 Msa1078580:CDS 55.0%
GACTGCAAGTTCTCCTACCG+AGG + chr7_2:77576801-77576820 None:intergenic 55.0%
AAGGGTGAGAATGGGTACCC+TGG - chr7_2:77576859-77576878 Msa1078580:intron 55.0%
CAATGCTCAGGAGGAAGCAG+GGG + chr7_2:77576898-77576917 None:intergenic 55.0%
! GAGGGATTTTACCGTAGCCG+AGG - chr7_2:77577217-77577236 Msa1078580:intron 55.0%
TTCGAGAAGCCAACCTTCCC+TGG + chr7_2:77577432-77577451 None:intergenic 55.0%
CAGAGCTGCGATTCCGATGG+CGG - chr7_2:77575225-77575244 Msa1078580:three_prime_UTR 60.0%
GGAGGAGAATCCACGCGCTA+AGG + chr7_2:77575290-77575309 None:intergenic 60.0%
CGGTGGCGCGTGAGAATATG+AGG + chr7_2:77575314-77575333 None:intergenic 60.0%
TTCTTAGGCTGTCCCGGCGT+TGG - chr7_2:77575361-77575380 Msa1078580:three_prime_UTR 60.0%
GAACACCGAGGAGGTTGCAG+AGG + chr7_2:77575408-77575427 None:intergenic 60.0%
CGATGTGAGGAACACCGAGG+AGG + chr7_2:77575417-77575436 None:intergenic 60.0%
GGAAGAAAGGGGGCCATTGG+AGG + chr7_2:77575470-77575489 None:intergenic 60.0%
CTGGGAAGAAAGGGGGCCAT+TGG + chr7_2:77575473-77575492 None:intergenic 60.0%
CAATGGCCCCCTTTCTTCCC+AGG - chr7_2:77575471-77575490 Msa1078580:three_prime_UTR 60.0%
CTGGCCGAAGGACAAGCCAA+AGG - chr7_2:77576499-77576518 Msa1078580:CDS 60.0%
TGAGCTTCCTGGAGGGATCC+CGG - chr7_2:77577255-77577274 Msa1078580:intron 60.0%
CAAGATTCCGGGATCCCTCC+AGG + chr7_2:77577265-77577284 None:intergenic 60.0%
CTGGAGGGATCCCGGAATCT+TGG - chr7_2:77577263-77577282 Msa1078580:intron 60.0%
!! AGGCGGGTTATGGCCGCCAT+CGG + chr7_2:77575241-77575260 None:intergenic 65.0%
ACGCGCCACCGCGTGATAAC+AGG - chr7_2:77575323-77575342 Msa1078580:three_prime_UTR 65.0%
!! GGCTGTCCCGGCGTTGGTAA+AGG - chr7_2:77575367-77575386 Msa1078580:three_prime_UTR 65.0%
AGCCGCCTCTGCAACCTCCT+CGG - chr7_2:77575400-77575419 Msa1078580:three_prime_UTR 65.0%
CACCGAGGAGGTTGCAGAGG+CGG + chr7_2:77575405-77575424 None:intergenic 65.0%
GTTGCAGAGGCGGCTGGCGT+AGG + chr7_2:77575395-77575414 None:intergenic 70.0%
GAGGAGGTTGCAGAGGCGGC+TGG + chr7_2:77575401-77575420 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr7_2 gene 77575145 77577865 77575145 ID=Msa1078580;Name=Msa1078580
chr7_2 mRNA 77575145 77577865 77575145 ID=Msa1078580-mRNA-1;Parent=Msa1078580;Name=Msa1078580-mRNA-1;_AED=0.07;_eAED=0.07;_QI=96|1|1|1|1|1|4|520|268
chr7_2 exon 77575145 77575805 77575145 ID=Msa1078580-mRNA-1:exon:19047;Parent=Msa1078580-mRNA-1
chr7_2 exon 77576017 77576289 77576017 ID=Msa1078580-mRNA-1:exon:19046;Parent=Msa1078580-mRNA-1
chr7_2 exon 77576440 77576580 77576440 ID=Msa1078580-mRNA-1:exon:19045;Parent=Msa1078580-mRNA-1
chr7_2 exon 77577518 77577865 77577518 ID=Msa1078580-mRNA-1:exon:19044;Parent=Msa1078580-mRNA-1
chr7_2 five_prime_UTR 77577770 77577865 77577770 ID=Msa1078580-mRNA-1:five_prime_utr;Parent=Msa1078580-mRNA-1
chr7_2 CDS 77577518 77577769 77577518 ID=Msa1078580-mRNA-1:cds;Parent=Msa1078580-mRNA-1
chr7_2 CDS 77576440 77576580 77576440 ID=Msa1078580-mRNA-1:cds;Parent=Msa1078580-mRNA-1
chr7_2 CDS 77576017 77576289 77576017 ID=Msa1078580-mRNA-1:cds;Parent=Msa1078580-mRNA-1
chr7_2 CDS 77575665 77575805 77575665 ID=Msa1078580-mRNA-1:cds;Parent=Msa1078580-mRNA-1
chr7_2 three_prime_UTR 77575145 77575664 77575145 ID=Msa1078580-mRNA-1:three_prime_utr;Parent=Msa1078580-mRNA-1
Gene Sequence

>Msa1078580

ATGGCGGCCATAACCCGCCTCGTTAAACGCACCACATCCTTTTATCCCTTAGCGCGTGGATTCTCCTCCTCCTCATATTCTCACGCGCCACCGCGTGATAACAGGAATATTCAGTGGGTTTTCTTAGGCTGTCCCGGCGTTGGTAAAGGTACCTACGCCAGCCGCCTCTGCAACCTCCTCGGTGTTCCTCACATCGCTACCGGAGATCTTGTTCGCCATGAGCTTTCCTCCAATGGCCCCCTTTCTTCCCAGCTATCAGAAATCGTAAATCAAGGCCAATTAGTGTCAGATGAAATTATTATGAGCTTGTTGTCTAAGAGACTGGCCGAAGGACAAGCCAAAGGCGAATCAGGTTTCATTCTTGATGGTTTTCCTCGAACAATAAAGCAAGCAGAAATATTGGAAGGGGTGACAGACATTGACTTGGTGGTCAATCTGAAGATCCGAGAAGAAGCACTGCTTGCAAAATGCCTCGGTAGGAGAACTTGCAGTCAGTGTGGAGGAAATTTTAATGTTGCCTCCATTAACATCAAGGGTGAGAATGGGTACCCTGGAATTAGTATGGCTCCCCTGCTTCCTCCTGAGCATTGTATGTCCAAGCTCATCACTCGCTCTGATGATACTGAACCAGTAGTCAAAGAAAGGCTTCGTATATACAATGAATTGAGTCAACCTGTGGAGGGATTTTACCGTAGCCGAGGAAAACTAATGGAGTTTGAGCTTCCTGGAGGGATCCCGGAATCTTGGCCAAAGTTGTTGCATGCTCTTAATCTAGATGATGAAGAGAAGCAGTCTGCTGCAGCATAA
Protein sequence

>Msa1078580

MAAITRLVKRTTSFYPLARGFSSSSYSHAPPRDNRNIQWVFLGCPGVGKGTYASRLCNLLGVPHIATGDLVRHELSSNGPLSSQLSEIVNQGQLVSDEIIMSLLSKRLAEGQAKGESGFILDGFPRTIKQAEILEGVTDIDLVVNLKIREEALLAKCLGRRTCSQCGGNFNVASINIKGENGYPGISMAPLLPPEHCMSKLITRSDDTEPVVKERLRIYNELSQPVEGFYRSRGKLMEFELPGGIPESWPKLLHALNLDDEEKQSAAA*