AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0688770


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0688770 MtrunA17_Chr4g0029791 97.872 94 2 0 1 94 1 94 6.33e-65 190
Msa0688770 MtrunA17_Chr2g0326261 45.361 97 27 4 1 94 1 74 1.80e-11 54.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0688770 AT5G60400.3 40.449 89 51 1 6 94 5 91 9.26e-12 56.2

Find 24 sgRNAs with CRISPR-Local

Find 80 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAGTTCCTCTCTGTCTTTC+TGG 0.075036 4_4:-54971836 Msa0688770:CDS
AAAATCAAATATGCATATTT+TGG 0.124229 4_4:-54971234 Msa0688770:three_prime_UTR
GTTTAACATGGACTGCATTT+TGG 0.222924 4_4:+54971874 None:intergenic
ACTTACTAGAATGTTAATTT+AGG 0.250075 4_4:+54971338 None:intergenic
AAAAGCTGCTTAGTTAGATT+TGG 0.304540 4_4:-54971471 Msa0688770:exon
CGAAGTTTCACCCTATTCAT+TGG 0.395651 4_4:+54971291 None:intergenic
TGATGATGATCTTGAAGATC+TGG 0.442771 4_4:-54971543 Msa0688770:CDS
TTCGTACGGACATATTTGTA+TGG 0.453259 4_4:-54971272 Msa0688770:three_prime_UTR
AGTGCATGCAAATCAAGAAA+AGG 0.472454 4_4:-54971609 Msa0688770:CDS
ACTGATGTGACCAATGAATA+GGG 0.474910 4_4:-54971301 Msa0688770:three_prime_UTR
AACTGATGTGACCAATGAAT+AGG 0.478716 4_4:-54971302 Msa0688770:three_prime_UTR
TTAAACCTTCGTGCTACTCA+TGG 0.498021 4_4:-54971857 Msa0688770:CDS
ACCGAAGAAGAAAAGATATG+AGG 0.524381 4_4:-54971643 Msa0688770:CDS
GTGCATGCAAATCAAGAAAA+GGG 0.524986 4_4:-54971608 Msa0688770:CDS
CTGTCTCTTACTTATCTCAC+AGG 0.529035 4_4:-54971503 Msa0688770:CDS
TACTCTTTGGAAAGAGCAAG+AGG 0.575505 4_4:+54971803 None:intergenic
AACATTCTAGTAAGTTCAGA+CGG 0.600784 4_4:-54971330 Msa0688770:three_prime_UTR
TTTGGAAAGAGCAAGAGGGT+TGG 0.601098 4_4:+54971808 None:intergenic
AAAAGGATGGGAAATAAAGG+AGG 0.624688 4_4:+54971925 None:intergenic
GAATAGGGTGAAACTTCGTA+CGG 0.636390 4_4:-54971286 Msa0688770:three_prime_UTR
ACTCTTTGGAAAGAGCAAGA+GGG 0.667434 4_4:+54971804 None:intergenic
AGTAGCACGAAGGTTTAACA+TGG 0.672060 4_4:+54971862 None:intergenic
GTGATTCCAGAAAGACAGAG+AGG 0.712435 4_4:+54971830 None:intergenic
GAACTCCATGAGTAGCACGA+AGG 0.732005 4_4:+54971852 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAAATTAATTTACTCTT+TGG + chr4_4:54971444-54971463 None:intergenic 10.0%
!! AAATAAATTAATTTACTCTT+TGG + chr4_4:54971444-54971463 None:intergenic 10.0%
!!! AAAATCAAATATGCATATTT+TGG - chr4_4:54971997-54972016 Msa0688770:five_prime_UTR 15.0%
!!! AAAATCAAATATGCATATTT+TGG - chr4_4:54971997-54972016 Msa0688770:five_prime_UTR 15.0%
!! TGATATATGTTTGAATTTGA+TGG - chr4_4:54971546-54971565 Msa0688770:CDS 20.0%
!! ACTTACTAGAATGTTAATTT+AGG + chr4_4:54971896-54971915 None:intergenic 20.0%
!!! ATGCATATTTGATTTTGATT+TGG + chr4_4:54971993-54972012 None:intergenic 20.0%
!! TGATATATGTTTGAATTTGA+TGG - chr4_4:54971546-54971565 Msa0688770:CDS 20.0%
!! ACTTACTAGAATGTTAATTT+AGG + chr4_4:54971896-54971915 None:intergenic 20.0%
!!! ATGCATATTTGATTTTGATT+TGG + chr4_4:54971993-54972012 None:intergenic 20.0%
!! TTTTCTAGGTAAAGGAAAAA+AGG + chr4_4:54971326-54971345 None:intergenic 25.0%
!!! GTTTTGTTTTTTCTAGGTAA+AGG + chr4_4:54971334-54971353 None:intergenic 25.0%
!!! TGGTTTGTTTTGTTTTTTCT+AGG + chr4_4:54971340-54971359 None:intergenic 25.0%
!! TCAAAGAAACAATGATTTTG+TGG - chr4_4:54971655-54971674 Msa0688770:CDS 25.0%
!! TCTAAATCACTTTCATTTTC+TGG - chr4_4:54971797-54971816 Msa0688770:intron 25.0%
!! TTTTCTAGGTAAAGGAAAAA+AGG + chr4_4:54971326-54971345 None:intergenic 25.0%
!!! GTTTTGTTTTTTCTAGGTAA+AGG + chr4_4:54971334-54971353 None:intergenic 25.0%
!!! TGGTTTGTTTTGTTTTTTCT+AGG + chr4_4:54971340-54971359 None:intergenic 25.0%
!! TCAAAGAAACAATGATTTTG+TGG - chr4_4:54971655-54971674 Msa0688770:CDS 25.0%
!! TCTAAATCACTTTCATTTTC+TGG - chr4_4:54971797-54971816 Msa0688770:intron 25.0%
GAAAAAAGGATGGGAAATAA+AGG + chr4_4:54971312-54971331 None:intergenic 30.0%
TAGGTAAAGGAAAAAAGGAT+GGG + chr4_4:54971321-54971340 None:intergenic 30.0%
! TCCTCATATCTTTTCTTCTT+CGG + chr4_4:54971592-54971611 None:intergenic 30.0%
! AATGATTTTGTGGAGATGAA+AGG - chr4_4:54971665-54971684 Msa0688770:intron 30.0%
AAAAGCTGCTTAGTTAGATT+TGG - chr4_4:54971760-54971779 Msa0688770:intron 30.0%
AACATTCTAGTAAGTTCAGA+CGG - chr4_4:54971901-54971920 Msa0688770:five_prime_UTR 30.0%
GAAAAAAGGATGGGAAATAA+AGG + chr4_4:54971312-54971331 None:intergenic 30.0%
TAGGTAAAGGAAAAAAGGAT+GGG + chr4_4:54971321-54971340 None:intergenic 30.0%
! TCCTCATATCTTTTCTTCTT+CGG + chr4_4:54971592-54971611 None:intergenic 30.0%
! AATGATTTTGTGGAGATGAA+AGG - chr4_4:54971665-54971684 Msa0688770:intron 30.0%
AAAAGCTGCTTAGTTAGATT+TGG - chr4_4:54971760-54971779 Msa0688770:intron 30.0%
AACATTCTAGTAAGTTCAGA+CGG - chr4_4:54971901-54971920 Msa0688770:five_prime_UTR 30.0%
AAAAGGATGGGAAATAAAGG+AGG + chr4_4:54971309-54971328 None:intergenic 35.0%
CTAGGTAAAGGAAAAAAGGA+TGG + chr4_4:54971322-54971341 None:intergenic 35.0%
!! GTTTAACATGGACTGCATTT+TGG + chr4_4:54971360-54971379 None:intergenic 35.0%
GTGAGATCATAGATCAAAAG+AGG + chr4_4:54971486-54971505 None:intergenic 35.0%
!! ATGTTTGAATTTGATGGTGC+AGG - chr4_4:54971552-54971571 Msa0688770:CDS 35.0%
ACCGAAGAAGAAAAGATATG+AGG - chr4_4:54971588-54971607 Msa0688770:CDS 35.0%
AGTGCATGCAAATCAAGAAA+AGG - chr4_4:54971622-54971641 Msa0688770:CDS 35.0%
GTGCATGCAAATCAAGAAAA+GGG - chr4_4:54971623-54971642 Msa0688770:CDS 35.0%
! TGATGATGATCTTGAAGATC+TGG - chr4_4:54971688-54971707 Msa0688770:intron 35.0%
AACTGATGTGACCAATGAAT+AGG - chr4_4:54971929-54971948 Msa0688770:five_prime_UTR 35.0%
ACTGATGTGACCAATGAATA+GGG - chr4_4:54971930-54971949 Msa0688770:five_prime_UTR 35.0%
TTCGTACGGACATATTTGTA+TGG - chr4_4:54971959-54971978 Msa0688770:five_prime_UTR 35.0%
AAAAGGATGGGAAATAAAGG+AGG + chr4_4:54971309-54971328 None:intergenic 35.0%
CTAGGTAAAGGAAAAAAGGA+TGG + chr4_4:54971322-54971341 None:intergenic 35.0%
!! GTTTAACATGGACTGCATTT+TGG + chr4_4:54971360-54971379 None:intergenic 35.0%
GTGAGATCATAGATCAAAAG+AGG + chr4_4:54971486-54971505 None:intergenic 35.0%
!! ATGTTTGAATTTGATGGTGC+AGG - chr4_4:54971552-54971571 Msa0688770:CDS 35.0%
ACCGAAGAAGAAAAGATATG+AGG - chr4_4:54971588-54971607 Msa0688770:CDS 35.0%
AGTGCATGCAAATCAAGAAA+AGG - chr4_4:54971622-54971641 Msa0688770:CDS 35.0%
GTGCATGCAAATCAAGAAAA+GGG - chr4_4:54971623-54971642 Msa0688770:CDS 35.0%
! TGATGATGATCTTGAAGATC+TGG - chr4_4:54971688-54971707 Msa0688770:intron 35.0%
AACTGATGTGACCAATGAAT+AGG - chr4_4:54971929-54971948 Msa0688770:five_prime_UTR 35.0%
ACTGATGTGACCAATGAATA+GGG - chr4_4:54971930-54971949 Msa0688770:five_prime_UTR 35.0%
TTCGTACGGACATATTTGTA+TGG - chr4_4:54971959-54971978 Msa0688770:five_prime_UTR 35.0%
AGTAGCACGAAGGTTTAACA+TGG + chr4_4:54971372-54971391 None:intergenic 40.0%
! TTAAACCTTCGTGCTACTCA+TGG - chr4_4:54971374-54971393 Msa0688770:three_prime_UTR 40.0%
! ACTCTTTGGAAAGAGCAAGA+GGG + chr4_4:54971430-54971449 None:intergenic 40.0%
! TACTCTTTGGAAAGAGCAAG+AGG + chr4_4:54971431-54971450 None:intergenic 40.0%
!! TTGAATTTGATGGTGCAGGA+TGG - chr4_4:54971556-54971575 Msa0688770:CDS 40.0%
CTGTCTCTTACTTATCTCAC+AGG - chr4_4:54971728-54971747 Msa0688770:intron 40.0%
CGAAGTTTCACCCTATTCAT+TGG + chr4_4:54971943-54971962 None:intergenic 40.0%
GAATAGGGTGAAACTTCGTA+CGG - chr4_4:54971945-54971964 Msa0688770:five_prime_UTR 40.0%
AGTAGCACGAAGGTTTAACA+TGG + chr4_4:54971372-54971391 None:intergenic 40.0%
! TTAAACCTTCGTGCTACTCA+TGG - chr4_4:54971374-54971393 Msa0688770:three_prime_UTR 40.0%
! ACTCTTTGGAAAGAGCAAGA+GGG + chr4_4:54971430-54971449 None:intergenic 40.0%
! TACTCTTTGGAAAGAGCAAG+AGG + chr4_4:54971431-54971450 None:intergenic 40.0%
!! TTGAATTTGATGGTGCAGGA+TGG - chr4_4:54971556-54971575 Msa0688770:CDS 40.0%
CTGTCTCTTACTTATCTCAC+AGG - chr4_4:54971728-54971747 Msa0688770:intron 40.0%
CGAAGTTTCACCCTATTCAT+TGG + chr4_4:54971943-54971962 None:intergenic 40.0%
GAATAGGGTGAAACTTCGTA+CGG - chr4_4:54971945-54971964 Msa0688770:five_prime_UTR 40.0%
GTGATTCCAGAAAGACAGAG+AGG + chr4_4:54971404-54971423 None:intergenic 45.0%
TTTGGAAAGAGCAAGAGGGT+TGG + chr4_4:54971426-54971445 None:intergenic 45.0%
GTGATTCCAGAAAGACAGAG+AGG + chr4_4:54971404-54971423 None:intergenic 45.0%
TTTGGAAAGAGCAAGAGGGT+TGG + chr4_4:54971426-54971445 None:intergenic 45.0%
GAACTCCATGAGTAGCACGA+AGG + chr4_4:54971382-54971401 None:intergenic 50.0%
GGAGTTCCTCTCTGTCTTTC+TGG - chr4_4:54971395-54971414 Msa0688770:three_prime_UTR 50.0%
GAACTCCATGAGTAGCACGA+AGG + chr4_4:54971382-54971401 None:intergenic 50.0%
GGAGTTCCTCTCTGTCTTTC+TGG - chr4_4:54971395-54971414 Msa0688770:three_prime_UTR 50.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 54971232 54972021 54971232 ID=Msa0688770;Name=Msa0688770
chr4_4 mRNA 54971232 54972021 54971232 ID=Msa0688770-mRNA-1;Parent=Msa0688770;Name=Msa0688770-mRNA-1;_AED=0.08;_eAED=0.08;_QI=130|1|1|1|1|1|2|249|94
chr4_4 exon 54971232 54971679 54971232 ID=Msa0688770-mRNA-1:exon:14514;Parent=Msa0688770-mRNA-1
chr4_4 exon 54971806 54972021 54971806 ID=Msa0688770-mRNA-1:exon:14513;Parent=Msa0688770-mRNA-1
chr4_4 five_prime_UTR 54971892 54972021 54971892 ID=Msa0688770-mRNA-1:five_prime_utr;Parent=Msa0688770-mRNA-1
chr4_4 CDS 54971806 54971891 54971806 ID=Msa0688770-mRNA-1:cds;Parent=Msa0688770-mRNA-1
chr4_4 CDS 54971481 54971679 54971481 ID=Msa0688770-mRNA-1:cds;Parent=Msa0688770-mRNA-1
chr4_4 three_prime_UTR 54971232 54971480 54971232 ID=Msa0688770-mRNA-1:three_prime_utr;Parent=Msa0688770-mRNA-1
Gene Sequence

>Msa0688770

ATGCAGTCCATGTTAAACCTTCGTGCTACTCATGGAGTTCCTCTCTGTCTTTCTGGAATCACCAACCCTCTTGCTCTTTCCAAAGAGATGGCAGATCACGACCGAAGAAGAAAAGATATGAGGAAGCAAAGATCAGTGCATGCAAATCAAGAAAAGGGTGCACAAAGTCAAAGAAACAATGATTTTGTGGAGATGAAAGGTGATGATGATCTTGAAGATCTGGATGAAATCTTGCATCAACTGTCTCTTACTTATCTCACAGGTTTATCAGCAAAAGCTGCTTAG

Protein sequence

>Msa0688770

MQSMLNLRATHGVPLCLSGITNPLALSKEMADHDRRRKDMRKQRSVHANQEKGAQSQRNNDFVEMKGDDDLEDLDEILHQLSLTYLTGLSAKAA*